                         SEQUENCE LISTING

<110>  Inserm
 
<120>  VACCINE COMPOSITIONS COMPRISING AN ATTENUATED MUTANT ZIKA VIRUS

<130>  BIO16125 DESPRES / MC

<140>  EP16305764.9
<141>  2016-06-23

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E glycoprotein from ZIKV strain BeH 819015 (Genbank access 
       #KU365778.1)

<400>  1

Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
1               5                   10                  15      


Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
            20                  25                  30          


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
        35                  40                  45              


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
    50                  55                  60                  


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
65                  70                  75                  80  


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
                85                  90                  95      


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
            100                 105                 110         


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
        115                 120                 125             


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
    130                 135                 140                 


Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
145                 150                 155                 160 


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
                165                 170                 175     


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
            180                 185                 190         


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
        195                 200                 205             


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
    210                 215                 220                 


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
225                 230                 235                 240 


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
                245                 250                 255     


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
            260                 265                 270         


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
        275                 280                 285             


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
    290                 295                 300                 


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
305                 310                 315                 320 


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
                325                 330                 335     


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
            340                 345                 350         


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
        355                 360                 365             


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
    370                 375                 380                 


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
385                 390                 395                 400 


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
                405                 410                 415     


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
            420                 425                 430         


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
        435                 440                 445             


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
    450                 455                 460                 


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
465                 470                 475                 480 


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
                485                 490                 495     


Phe Leu Ser Thr Ala Val Ser Ala 
            500                 


<210>  2
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The E sequences of mutant [E-I152T, E-T156I, E-H158Y] of 
       ZIKVBR15-MC

<400>  2

Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
1               5                   10                  15      


Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
            20                  25                  30          


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
        35                  40                  45              


Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
    50                  55                  60                  


Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
65                  70                  75                  80  


Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
                85                  90                  95      


Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
            100                 105                 110         


Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
        115                 120                 125             


Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
    130                 135                 140                 


Gly Ser Gln His Ser Gly Met Thr Val Asn Asp Ile Gly Tyr Glu Thr 
145                 150                 155                 160 


Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
                165                 170                 175     


Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
            180                 185                 190         


Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
        195                 200                 205             


Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
    210                 215                 220                 


Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
225                 230                 235                 240 


Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
                245                 250                 255     


Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
            260                 265                 270         


Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
        275                 280                 285             


Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
    290                 295                 300                 


Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
305                 310                 315                 320 


Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
                325                 330                 335     


Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
            340                 345                 350         


Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
        355                 360                 365             


Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
    370                 375                 380                 


Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
385                 390                 395                 400 


His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
                405                 410                 415     


Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
            420                 425                 430         


Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
        435                 440                 445             


Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
    450                 455                 460                 


Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
465                 470                 475                 480 


Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
                485                 490                 495     


Phe Leu Ser Thr Ala Val Ser Ala 
            500                 


<210>  3
<211>  3423
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polyprotein sequence of chimeric ZIKVBR15-MC mutant [E-I152T, 
       E-T156I, E-H158Y ] (3,423 aa)

<400>  3

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
    130                 135                 140                 


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Thr Val Asn Asp Ile Gly Tyr 
        435                 440                 445             


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
    450                 455                 460                 


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
465                 470                 475                 480 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
                485                 490                 495     


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
            500                 505                 510         


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
        515                 520                 525             


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
    530                 535                 540                 


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
545                 550                 555                 560 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
            580                 585                 590         


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
        595                 600                 605             


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
    610                 615                 620                 


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
625                 630                 635                 640 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
                645                 650                 655     


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
        675                 680                 685             


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
    690                 695                 700                 


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
                725                 730                 735     


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
        755                 760                 765             


Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
    770                 775                 780                 


Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
785                 790                 795                 800 


Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr 
                805                 810                 815     


Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser 
            820                 825                 830         


Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile 
        835                 840                 845             


Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser 
    850                 855                 860                 


Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu 
865                 870                 875                 880 


Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln 
                885                 890                 895     


Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp 
            900                 905                 910         


Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val 
        915                 920                 925             


Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp 
    930                 935                 940                 


Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser 
945                 950                 955                 960 


Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala 
                965                 970                 975     


Val Ile Gly Thr Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu 
            980                 985                 990         


Gly Tyr Trp Ile Glu Ser Glu Lys  Asn Asp Thr Trp Arg  Leu Lys Arg 
        995                 1000                 1005             


Ala His  Leu Ile Glu Met Lys  Thr Cys Glu Trp Pro  Lys Ser His 
    1010                 1015                 1020             


Thr Leu  Trp Thr Asp Gly Ile  Glu Glu Ser Asp Leu  Ile Ile Pro 
    1025                 1030                 1035             


Lys Ser  Leu Ala Gly Pro Leu  Ser His His Asn Thr  Arg Glu Gly 
    1040                 1045                 1050             


Tyr Arg  Thr Gln Met Lys Gly  Pro Trp His Ser Glu  Glu Leu Glu 
    1055                 1060                 1065             


Ile Arg  Phe Glu Glu Cys Pro  Gly Thr Lys Val His  Val Glu Glu 
    1070                 1075                 1080             


Thr Cys  Gly Thr Arg Gly Pro  Ser Leu Arg Ser Thr  Thr Ala Ser 
    1085                 1090                 1095             


Gly Arg  Val Ile Glu Glu Trp  Cys Cys Arg Glu Cys  Thr Met Pro 
    1100                 1105                 1110             


Pro Leu  Ser Phe Arg Ala Lys  Asp Gly Cys Trp Tyr  Gly Met Glu 
    1115                 1120                 1125             


Ile Arg  Pro Arg Lys Glu Pro  Glu Ser Asn Leu Val  Arg Ser Met 
    1130                 1135                 1140             


Val Thr  Ala Gly Ser Thr Asp  His Met Asp His Phe  Ser Leu Gly 
    1145                 1150                 1155             


Val Leu  Val Ile Leu Leu Met  Val Gln Glu Gly Leu  Lys Lys Arg 
    1160                 1165                 1170             


Met Thr  Thr Lys Ile Ile Ile  Ser Thr Ser Met Ala  Val Leu Val 
    1175                 1180                 1185             


Ala Met  Ile Leu Gly Gly Phe  Ser Met Ser Asp Leu  Ala Lys Leu 
    1190                 1195                 1200             


Ala Ile  Leu Met Gly Ala Thr  Phe Ala Glu Met Asn  Thr Gly Gly 
    1205                 1210                 1215             


Asp Val  Ala His Leu Ala Leu  Ile Ala Ala Phe Lys  Val Arg Pro 
    1220                 1225                 1230             


Ala Leu  Leu Val Ser Phe Ile  Phe Arg Ala Asn Trp  Thr Pro Arg 
    1235                 1240                 1245             


Glu Ser  Met Leu Leu Ala Leu  Ala Ser Cys Leu Leu  Gln Thr Ala 
    1250                 1255                 1260             


Ile Ser  Ala Leu Glu Gly Asp  Leu Met Val Leu Ile  Asn Gly Phe 
    1265                 1270                 1275             


Ala Leu  Ala Trp Leu Ala Ile  Arg Ala Met Val Val  Pro Arg Thr 
    1280                 1285                 1290             


Asp Asn  Ile Thr Leu Ala Ile  Leu Ala Ala Leu Thr  Pro Leu Ala 
    1295                 1300                 1305             


Arg Gly  Thr Leu Leu Val Ala  Trp Arg Ala Gly Leu  Ala Thr Cys 
    1310                 1315                 1320             


Gly Gly  Phe Met Leu Leu Ser  Leu Lys Gly Lys Gly  Ser Val Lys 
    1325                 1330                 1335             


Lys Asn  Leu Pro Phe Val Met  Ala Leu Gly Leu Thr  Ala Val Arg 
    1340                 1345                 1350             


Leu Val  Asp Pro Ile Asn Val  Val Gly Leu Leu Leu  Leu Thr Arg 
    1355                 1360                 1365             


Ser Gly  Lys Arg Ser Trp Pro  Pro Ser Glu Val Leu  Thr Ala Val 
    1370                 1375                 1380             


Gly Leu  Ile Cys Ala Leu Ala  Gly Gly Phe Ala Lys  Ala Asp Ile 
    1385                 1390                 1395             


Glu Met  Ala Gly Pro Met Ala  Ala Val Gly Leu Leu  Ile Val Ser 
    1400                 1405                 1410             


Tyr Val  Val Ser Gly Lys Ser  Val Asp Met Tyr Ile  Glu Arg Ala 
    1415                 1420                 1425             


Gly Asp  Ile Thr Trp Glu Lys  Asp Ala Glu Val Thr  Gly Asn Ser 
    1430                 1435                 1440             


Pro Arg  Leu Asp Val Ala Leu  Asp Glu Ser Gly Asp  Phe Ser Leu 
    1445                 1450                 1455             


Val Glu  Asp Asp Gly Pro Pro  Met Arg Glu Ile Ile  Leu Lys Val 
    1460                 1465                 1470             


Val Leu  Met Thr Ile Cys Gly  Met Asn Pro Ile Ala  Ile Pro Phe 
    1475                 1480                 1485             


Ala Ala  Gly Ala Trp Tyr Val  Tyr Val Lys Thr Gly  Lys Arg Ser 
    1490                 1495                 1500             


Gly Ala  Leu Trp Asp Val Pro  Ala Pro Lys Glu Val  Lys Lys Gly 
    1505                 1510                 1515             


Glu Thr  Thr Asp Gly Val Tyr  Arg Val Met Thr Arg  Arg Leu Leu 
    1520                 1525                 1530             


Gly Ser  Thr Gln Val Gly Val  Gly Val Met Gln Glu  Gly Val Phe 
    1535                 1540                 1545             


His Thr  Met Trp His Val Thr  Lys Gly Ser Ala Leu  Arg Ser Gly 
    1550                 1555                 1560             


Glu Gly  Arg Leu Asp Pro Tyr  Trp Gly Asp Val Lys  Gln Asp Leu 
    1565                 1570                 1575             


Val Ser  Tyr Cys Gly Pro Trp  Lys Leu Asp Ala Ala  Trp Asp Gly 
    1580                 1585                 1590             


His Ser  Glu Val Gln Leu Leu  Ala Val Pro Pro Gly  Glu Arg Ala 
    1595                 1600                 1605             


Arg Asn  Ile Gln Thr Leu Pro  Gly Ile Phe Lys Thr  Lys Asp Gly 
    1610                 1615                 1620             


Asp Ile  Gly Ala Val Ala Leu  Asp Tyr Pro Ala Gly  Thr Ser Gly 
    1625                 1630                 1635             


Ser Pro  Ile Leu Asp Lys Cys  Gly Arg Val Ile Gly  Leu Tyr Gly 
    1640                 1645                 1650             


Asn Gly  Val Val Ile Lys Asn  Gly Ser Tyr Val Ser  Ala Ile Thr 
    1655                 1660                 1665             


Gln Gly  Arg Arg Glu Glu Glu  Thr Pro Val Glu Cys  Phe Glu Pro 
    1670                 1675                 1680             


Ser Met  Leu Lys Lys Lys Gln  Leu Thr Val Leu Asp  Leu His Pro 
    1685                 1690                 1695             


Gly Ala  Gly Lys Thr Arg Arg  Val Leu Pro Glu Ile  Val Arg Glu 
    1700                 1705                 1710             


Ala Ile  Lys Thr Arg Leu Arg  Thr Val Ile Leu Ala  Pro Thr Arg 
    1715                 1720                 1725             


Val Val  Ala Ala Glu Met Glu  Glu Ala Leu Arg Gly  Leu Pro Val 
    1730                 1735                 1740             


Arg Tyr  Met Thr Thr Ala Val  Asn Val Thr His Ser  Gly Thr Glu 
    1745                 1750                 1755             


Ile Val  Asp Leu Met Cys His  Ala Thr Phe Thr Ser  Arg Leu Leu 
    1760                 1765                 1770             


Gln Pro  Ile Arg Val Pro Asn  Tyr Asn Leu Tyr Ile  Met Asp Glu 
    1775                 1780                 1785             


Ala His  Phe Thr Asp Pro Ser  Ser Ile Ala Ala Arg  Gly Tyr Ile 
    1790                 1795                 1800             


Ser Thr  Arg Val Glu Met Gly  Glu Ala Ala Ala Ile  Phe Met Thr 
    1805                 1810                 1815             


Ala Thr  Pro Pro Gly Thr Arg  Asp Ala Phe Pro Asp  Ser Asn Ser 
    1820                 1825                 1830             


Pro Ile  Met Asp Thr Glu Val  Glu Val Pro Glu Arg  Ala Trp Ser 
    1835                 1840                 1845             


Ser Gly  Phe Asp Trp Val Thr  Asp His Ser Gly Lys  Thr Val Trp 
    1850                 1855                 1860             


Phe Val  Pro Ser Val Arg Asn  Gly Asn Glu Ile Ala  Ala Cys Leu 
    1865                 1870                 1875             


Thr Lys  Ala Gly Lys Arg Val  Ile Gln Leu Ser Arg  Lys Thr Phe 
    1880                 1885                 1890             


Glu Thr  Glu Phe Gln Lys Thr  Lys His Gln Glu Trp  Asp Phe Val 
    1895                 1900                 1905             


Val Thr  Thr Asp Ile Ser Glu  Met Gly Ala Asn Phe  Lys Ala Asp 
    1910                 1915                 1920             


Arg Val  Ile Asp Ser Arg Arg  Cys Leu Lys Pro Val  Ile Leu Asp 
    1925                 1930                 1935             


Gly Glu  Arg Val Ile Leu Ala  Gly Pro Met Pro Val  Thr His Ala 
    1940                 1945                 1950             


Ser Ala  Ala Gln Arg Arg Gly  Arg Ile Gly Arg Asn  Pro Asn Lys 
    1955                 1960                 1965             


Pro Gly  Asp Glu Tyr Leu Tyr  Gly Gly Gly Cys Ala  Glu Thr Asp 
    1970                 1975                 1980             


Glu Asp  His Ala His Trp Leu  Glu Ala Arg Met Leu  Leu Asp Asn 
    1985                 1990                 1995             


Ile Tyr  Leu Gln Asp Gly Leu  Ile Ala Ser Leu Tyr  Arg Pro Glu 
    2000                 2005                 2010             


Ala Asp  Lys Val Ala Ala Ile  Glu Gly Glu Phe Lys  Leu Arg Thr 
    2015                 2020                 2025             


Glu Gln  Arg Lys Thr Phe Val  Glu Leu Met Lys Arg  Gly Asp Leu 
    2030                 2035                 2040             


Pro Val  Trp Leu Ala Tyr Gln  Val Ala Ser Ala Gly  Ile Thr Tyr 
    2045                 2050                 2055             


Thr Asp  Arg Arg Trp Cys Phe  Asp Gly Thr Thr Asn  Asn Thr Ile 
    2060                 2065                 2070             


Met Glu  Asp Ser Val Pro Ala  Glu Val Trp Thr Arg  His Gly Glu 
    2075                 2080                 2085             


Lys Arg  Val Leu Lys Pro Arg  Trp Met Asp Ala Arg  Val Cys Ser 
    2090                 2095                 2100             


Asp His  Ala Ala Leu Lys Ser  Phe Lys Glu Phe Ala  Ala Gly Lys 
    2105                 2110                 2115             


Arg Gly  Ala Ala Phe Gly Val  Met Glu Ala Leu Gly  Thr Leu Pro 
    2120                 2125                 2130             


Gly His  Met Thr Glu Arg Phe  Gln Glu Ala Ile Asp  Asn Leu Ala 
    2135                 2140                 2145             


Val Leu  Met Arg Ala Glu Thr  Gly Ser Arg Pro Tyr  Lys Ala Ala 
    2150                 2155                 2160             


Ala Ala  Gln Leu Pro Glu Thr  Leu Glu Thr Ile Met  Leu Leu Gly 
    2165                 2170                 2175             


Leu Leu  Gly Thr Val Ser Leu  Gly Ile Phe Phe Val  Leu Met Arg 
    2180                 2185                 2190             


Asn Lys  Gly Ile Gly Lys Met  Gly Phe Gly Met Val  Thr Leu Gly 
    2195                 2200                 2205             


Ala Ser  Ala Trp Leu Met Trp  Leu Ser Glu Ile Glu  Pro Ala Arg 
    2210                 2215                 2220             


Ile Ala  Cys Val Leu Ile Val  Val Phe Leu Leu Leu  Val Val Leu 
    2225                 2230                 2235             


Ile Pro  Glu Pro Glu Lys Gln  Arg Ser Pro Gln Asp  Asn Gln Met 
    2240                 2245                 2250             


Ala Ile  Ile Ile Met Val Ala  Val Gly Leu Leu Gly  Leu Ile Thr 
    2255                 2260                 2265             


Ala Asn  Glu Leu Gly Trp Leu  Glu Arg Thr Lys Ser  Asp Leu Ser 
    2270                 2275                 2280             


His Leu  Met Gly Arg Arg Glu  Glu Gly Ala Thr Ile  Gly Phe Ser 
    2285                 2290                 2295             


Met Asp  Ile Asp Leu Arg Pro  Ala Ser Ala Trp Ala  Ile Tyr Ala 
    2300                 2305                 2310             


Ala Leu  Thr Thr Phe Ile Thr  Pro Ala Val Gln His  Ala Val Thr 
    2315                 2320                 2325             


Thr Ser  Tyr Asn Asn Tyr Ser  Leu Met Ala Met Ala  Thr Gln Ala 
    2330                 2335                 2340             


Gly Val  Leu Phe Gly Met Gly  Lys Gly Met Pro Phe  Tyr Ala Trp 
    2345                 2350                 2355             


Asp Phe  Gly Val Pro Leu Leu  Met Ile Gly Cys Tyr  Ser Gln Leu 
    2360                 2365                 2370             


Thr Pro  Leu Thr Leu Ile Val  Ala Ile Ile Leu Leu  Val Ala His 
    2375                 2380                 2385             


Tyr Met  Tyr Leu Ile Pro Gly  Leu Gln Ala Ala Ala  Ala Arg Ala 
    2390                 2395                 2400             


Ala Gln  Lys Arg Thr Ala Ala  Gly Ile Met Lys Asn  Pro Val Val 
    2405                 2410                 2415             


Asp Gly  Ile Val Val Thr Asp  Ile Asp Thr Met Thr  Ile Asp Pro 
    2420                 2425                 2430             


Gln Val  Glu Lys Lys Met Gly  Gln Val Leu Leu Ile  Ala Val Ala 
    2435                 2440                 2445             


Val Ser  Ser Ala Ile Leu Ser  Arg Thr Ala Trp Gly  Trp Gly Glu 
    2450                 2455                 2460             


Ala Gly  Ala Leu Ile Thr Ala  Ala Thr Ser Thr Leu  Trp Glu Gly 
    2465                 2470                 2475             


Ser Pro  Asn Lys Tyr Trp Asn  Ser Ser Thr Ala Thr  Ser Leu Cys 
    2480                 2485                 2490             


Asn Ile  Phe Arg Gly Ser Tyr  Leu Ala Gly Ala Ser  Leu Ile Tyr 
    2495                 2500                 2505             


Thr Val  Thr Arg Asn Ala Gly  Leu Val Lys Arg Arg  Gly Gly Gly 
    2510                 2515                 2520             


Thr Gly  Glu Thr Leu Gly Glu  Lys Trp Lys Ala Arg  Leu Asn Gln 
    2525                 2530                 2535             


Met Ser  Ala Leu Glu Phe Tyr  Ser Tyr Lys Lys Ser  Gly Ile Thr 
    2540                 2545                 2550             


Glu Val  Cys Arg Glu Glu Ala  Arg Arg Ala Leu Lys  Asp Gly Val 
    2555                 2560                 2565             


Ala Thr  Gly Gly His Ala Val  Ser Arg Gly Ser Ala  Lys Leu Arg 
    2570                 2575                 2580             


Trp Leu  Val Glu Arg Gly Tyr  Leu Gln Pro Tyr Gly  Lys Val Ile 
    2585                 2590                 2595             


Asp Leu  Gly Cys Gly Arg Gly  Gly Trp Ser Tyr Tyr  Ala Ala Thr 
    2600                 2605                 2610             


Ile Arg  Lys Val Gln Glu Val  Lys Gly Tyr Thr Lys  Gly Gly Pro 
    2615                 2620                 2625             


Gly His  Glu Glu Pro Val Leu  Val Gln Ser Tyr Gly  Trp Asn Ile 
    2630                 2635                 2640             


Val Arg  Leu Lys Ser Gly Val  Asp Val Phe His Met  Ala Ala Glu 
    2645                 2650                 2655             


Pro Cys  Asp Thr Leu Leu Cys  Asp Ile Gly Glu Ser  Ser Ser Ser 
    2660                 2665                 2670             


Pro Glu  Val Glu Glu Ala Arg  Thr Leu Arg Val Leu  Ser Met Val 
    2675                 2680                 2685             


Gly Asp  Trp Leu Glu Lys Arg  Pro Gly Ala Phe Cys  Ile Lys Val 
    2690                 2695                 2700             


Leu Cys  Pro Tyr Thr Ser Thr  Met Met Glu Thr Leu  Glu Arg Leu 
    2705                 2710                 2715             


Gln Arg  Arg Tyr Gly Gly Gly  Leu Val Arg Val Pro  Leu Ser Arg 
    2720                 2725                 2730             


Asn Ser  Thr His Glu Met Tyr  Trp Val Ser Gly Ala  Lys Ser Asn 
    2735                 2740                 2745             


Thr Ile  Lys Ser Val Ser Thr  Thr Ser Gln Leu Leu  Leu Gly Arg 
    2750                 2755                 2760             


Met Asp  Gly Pro Arg Arg Pro  Val Lys Tyr Glu Glu  Asp Val Asn 
    2765                 2770                 2775             


Leu Gly  Ser Gly Thr Arg Ala  Val Val Ser Cys Ala  Glu Ala Pro 
    2780                 2785                 2790             


Asn Met  Lys Ile Ile Gly Asn  Arg Ile Glu Arg Ile  Arg Ser Glu 
    2795                 2800                 2805             


His Ala  Glu Thr Trp Phe Phe  Asp Glu Asn His Pro  Tyr Arg Thr 
    2810                 2815                 2820             


Trp Ala  Tyr His Gly Ser Tyr  Glu Ala Pro Thr Gln  Gly Ser Ala 
    2825                 2830                 2835             


Ser Ser  Leu Ile Asn Gly Val  Val Arg Leu Leu Ser  Lys Pro Trp 
    2840                 2845                 2850             


Asp Val  Val Thr Gly Val Thr  Gly Ile Ala Met Thr  Asp Thr Thr 
    2855                 2860                 2865             


Pro Tyr  Gly Gln Gln Arg Val  Phe Lys Glu Lys Val  Asp Thr Arg 
    2870                 2875                 2880             


Val Pro  Asp Pro Gln Glu Gly  Thr Arg Gln Val Met  Ser Met Val 
    2885                 2890                 2895             


Ser Ser  Trp Leu Trp Lys Glu  Leu Gly Lys His Lys  Arg Pro Arg 
    2900                 2905                 2910             


Val Cys  Thr Lys Glu Glu Phe  Ile Asn Lys Val Arg  Ser Asn Ala 
    2915                 2920                 2925             


Ala Leu  Gly Ala Ile Phe Glu  Glu Glu Lys Glu Trp  Lys Thr Ala 
    2930                 2935                 2940             


Val Glu  Ala Val Asn Asp Pro  Arg Phe Trp Ala Leu  Val Asp Lys 
    2945                 2950                 2955             


Glu Arg  Glu His His Leu Arg  Gly Glu Cys Gln Ser  Cys Val Tyr 
    2960                 2965                 2970             


Asn Met  Met Gly Lys Arg Glu  Lys Lys Gln Gly Glu  Phe Gly Lys 
    2975                 2980                 2985             


Ala Lys  Gly Ser Arg Ala Ile  Trp Tyr Met Trp Leu  Gly Ala Arg 
    2990                 2995                 3000             


Phe Leu  Glu Phe Glu Ala Leu  Gly Phe Leu Asn Glu  Asp His Trp 
    3005                 3010                 3015             


Met Gly  Arg Glu Asn Ser Gly  Gly Gly Val Glu Gly  Leu Gly Leu 
    3020                 3025                 3030             


Gln Arg  Leu Gly Tyr Val Leu  Glu Glu Met Ser Arg  Ile Pro Gly 
    3035                 3040                 3045             


Gly Arg  Met Tyr Ala Asp Asp  Thr Ala Gly Trp Asp  Thr Arg Ile 
    3050                 3055                 3060             


Ser Arg  Phe Asp Leu Glu Asn  Glu Ala Leu Ile Thr  Asn Gln Met 
    3065                 3070                 3075             


Glu Lys  Gly His Arg Ala Leu  Ala Leu Ala Ile Ile  Lys Tyr Thr 
    3080                 3085                 3090             


Tyr Gln  Asn Lys Val Val Lys  Val Leu Arg Pro Ala  Glu Lys Gly 
    3095                 3100                 3105             


Lys Thr  Val Met Asp Ile Ile  Ser Arg Gln Asp Gln  Arg Gly Ser 
    3110                 3115                 3120             


Gly Gln  Val Val Thr Tyr Ala  Leu Asn Thr Phe Thr  Asn Leu Val 
    3125                 3130                 3135             


Val Gln  Leu Ile Arg Asn Met  Glu Ala Glu Glu Val  Leu Glu Met 
    3140                 3145                 3150             


Gln Asp  Leu Trp Leu Leu Arg  Arg Ser Glu Lys Val  Thr Asn Trp 
    3155                 3160                 3165             


Leu Gln  Ser Asn Gly Trp Asp  Arg Leu Lys Arg Met  Ala Val Ser 
    3170                 3175                 3180             


Gly Asp  Asp Cys Val Val Lys  Pro Ile Asp Asp Arg  Phe Ala His 
    3185                 3190                 3195             


Ala Leu  Arg Phe Leu Asn Asp  Met Gly Lys Val Arg  Lys Asp Thr 
    3200                 3205                 3210             


Gln Glu  Trp Lys Pro Ser Thr  Gly Trp Asp Asn Trp  Glu Glu Val 
    3215                 3220                 3225             


Pro Phe  Cys Ser His His Phe  Asn Lys Leu His Leu  Lys Asp Gly 
    3230                 3235                 3240             


Arg Ser  Ile Val Val Pro Cys  Arg His Gln Asp Glu  Leu Ile Gly 
    3245                 3250                 3255             


Arg Ala  Arg Val Ser Pro Gly  Ala Gly Trp Ser Ile  Arg Glu Thr 
    3260                 3265                 3270             


Ala Cys  Leu Ala Lys Ser Tyr  Ala Gln Met Trp Gln  Leu Leu Tyr 
    3275                 3280                 3285             


Phe His  Arg Arg Asp Leu Arg  Leu Met Ala Asn Ala  Ile Cys Ser 
    3290                 3295                 3300             


Ser Val  Pro Val Asp Trp Val  Pro Thr Gly Arg Thr  Thr Trp Ser 
    3305                 3310                 3315             


Ile His  Gly Lys Gly Glu Trp  Met Thr Thr Glu Asp  Met Leu Val 
    3320                 3325                 3330             


Val Trp  Asn Arg Val Trp Ile  Glu Glu Asn Asp His  Met Glu Asp 
    3335                 3340                 3345             


Lys Thr  Pro Val Thr Lys Trp  Thr Asp Ile Pro Tyr  Leu Gly Lys 
    3350                 3355                 3360             


Arg Glu  Asp Leu Trp Cys Gly  Ser Leu Ile Gly His  Arg Pro Arg 
    3365                 3370                 3375             


Thr Thr  Trp Ala Glu Asn Ile  Lys Asn Thr Val Asn  Met Val Arg 
    3380                 3385                 3390             


Arg Ile  Ile Gly Asp Glu Glu  Lys Tyr Met Asp Tyr  Leu Ser Thr 
    3395                 3400                 3405             


Gln Val  Arg Tyr Leu Gly Glu  Glu Gly Ser Thr Pro  Gly Val Leu 
    3410                 3415                 3420             


<210>  4
<211>  10807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genomic sequence of chimeric ZIKVBR15-MC mutant [E-I152T, 
       E-T156I, E-H158Y ] (10,807 nt)

<400>  4
agttgttgat ctgtgtgagt cagactgcga cagttcgagt ctgaagcgag agctaacaac       60

agtatcaaca ggtttaattt ggatttggaa acgagagttt ctggtcatga aaaacccaaa      120

aaagaaatcc ggaggattcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtgag      180

cccctttggg ggcttgaaga ggctgccagc cggacttctg ctgggtcatg ggcccatcag      240

gatggtcttg gcgattctag cctttttgag attcacggca atcaagccat cactgggtct      300

catcaataga tggggttcag ttgggaaaaa agaggctatg gaaataataa agaagttcaa      360

gaaagatctg gctgccatgc tgagaataat caatgctagg aaggagaaga agagacgagg      420

cgcagatact agtgtcggaa ttgttggcct cctgctgacc acagctatgg cagcggaggt      480

cactagacgt gggagtgcat actatatgta cttggacaga aacgatgctg gggaggccat      540

atcttttcca accacattgg ggatgaataa gtgttatata cagatcatgg atcttggaca      600

catgtgtgat gccaccatga gctatgaatg ccctatgctg gatgaggggg tggaaccaga      660

tgacgtcgat tgttggtgca acacgacgtc aacttgggtt gtgtacggaa cctgccatca      720

caaaaaaggt gaagcacgga gatctagaag agctgtgacg ctcccctccc attccactag      780

gaagctgcaa acgcggtcgc aaacctggtt ggaatcaaga gaatacacaa agcacttgat      840

tagagtcgaa aattggatat tcaggaaccc tggcttcgcg ttagcagcag ctgccatcgc      900

ttggcttttg ggaagctcaa cgagccaaaa agtcatatac ttggtcatga tactgctgat      960

tgccccggca tacagcatca ggtgcatagg agtcagcaat agggactttg tggaaggtat     1020

gtcaggtggg acttgggttg atgttgtctt ggaacatgga ggttgtgtca ccgtaatggc     1080

acaggacaaa ccgactgtcg acatagagct ggttacaaca acagtcagca acatggcgga     1140

ggtacgatcg tactgctatg aggcatcaat atcagacatg gcttcggaca gccgctgccc     1200

aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac     1260

gttagtggac agaggctggg gaaatggatg tggacttttt ggcaaaggga gcctggtgac     1320

atgcgctaag tttgcatgct ccaagaaaat gaccgggaag agcatccagc cagagaatct     1380

ggagtaccgg ataatgctgt cagttcatgg ctcccagcac agtgggatga ctgtcaatga     1440

tataggatat gaaactgatg agaatagagc gaaagttgag ataacgccca attcaccaag     1500

agccgaagcc accctggggg gttttggaag cctaggactt gattgtgaac cgaggacagg     1560

ccttgacttt tcagatttgt attacttgac tatgaataac aagcactggt tggttcacaa     1620

ggagtggttc cacgacattc cattaccttg gcacgctggg gcagacaccg gaactccaca     1680

ctggaacaac aaagaagcac tggtagagtt caaggacgca catgccaaaa ggcaaactgt     1740

cgtggttcta gggagtcaag aaggagcagt tcacacggcc cttgctggag ctctggaggc     1800

tgagatggat ggtgcaaagg gaaggctgtc ctctggccac ttgaaatgtc gcctgaaaat     1860

ggataaactt agattgaagg gcgtgtcata ctccttgtgt actgcagcgt tcacattcac     1920

caagatcccg gctgaaacac tgcacgggac agtcacagtg gaggtacagt acgcagggac     1980

agatggacct tgcaaggttc cagctcagat ggcggtggac atgcaaactc tgaccccagt     2040

tgggaggttg ataaccgcta accccgtaat cactgaaagc actgagaact ctaagatgat     2100

gctggaactt gatccaccat ttggggactc ttacattgtc ataggagtcg gggagaagaa     2160

gatcacccac cactggcaca ggagtggcag caccattgga aaagcatttg aggccactgt     2220

gagaggcgcc aagagaatgg cagtcctggg ggacacagcc tgggactttg gatcagttgg     2280

aggcgctctc aactcattgg gcaagggcat ccatcaaatt tttggagcag ctttcaaatc     2340

attgtttgga ggaatgtcct ggttctcaca aattctcatt ggaacgttgc tgatgtggtt     2400

gggtctgaac acaaagaatg gatctatttc ccttatgtgc ttggccttag ggggggtgtt     2460

gatcttctta tccacagccg tctctgctga tgtggggtgc tcggtggact tctcaaagaa     2520

ggagacgaga tgcggtacag gggtgttcgt ctataacgac gttgaagcct ggagggacag     2580

gtacaagtac catcctgact ccccccgtag attggcagca gcagtcaagc aagcctggga     2640

agatggtatc tgcgggatct cctctgtttc aagaatggaa aacatcatgt ggagatcagt     2700

agaaggggag ctcaacgcaa tcctggaaga gaatggagtt caactgacgg tcgttgtggg     2760

atctgtaaaa aaccccatgt ggagaggtcc acagagattg cccgtgcctg tgaacgagct     2820

gccccacggc tggaaggctt gggggaaatc gtacttcgtc agagcagcaa agacaaataa     2880

cagctttgtc gtggatggtg acacactgaa ggaatgccca ctcaaacata gagcatggaa     2940

cagctttctt gtggaggatc atgggttcgg ggtatttcac actagtgtct ggctcaaggt     3000

tagagaagat tattcattag agtgtgatcc agccgttatt ggaacagctg ttaagggaaa     3060

ggaggctgta cacagtgatc taggctactg gattgagagt gagaagaatg acacatggag     3120

gctgaagagg gcccatctga tcgagatgaa aacatgtgaa tggccaaagt cccacacatt     3180

gtggacagat ggaatagaag agagtgatct gatcataccc aagtctttag ctgggccact     3240

cagccatcac aataccagag agggctacag gacccaaatg aaagggccat ggcacagtga     3300

agagcttgaa attcggtttg aggaatgccc aggcactaag gtccacgtgg aggaaacatg     3360

tggaacaaga ggaccatctc tgagatcaac cactgcaagc ggaagggtga tcgaggaatg     3420

gtgctgcagg gagtgcacaa tgcccccact gtcgttccgg gctaaagatg gctgttggta     3480

tggaatggag ataaggccca ggaaagaacc agaaagcaac ttagtaaggt caatggtgac     3540

tgcaggatca actgatcaca tggaccactt ctcccttgga gtgcttgtga tcctgctcat     3600

ggtgcaggaa gggctgaaga agagaatgac cacaaagatc atcataagca catcaatggc     3660

agtgctggta gctatgatcc tgggaggatt ttcaatgagt gacctggcta agcttgcaat     3720

tttgatgggt gccaccttcg cggaaatgaa cactggagga gatgtagctc atctggcgct     3780

gatagcggca ttcaaagtca gaccagcgtt gctggtatct ttcatcttca gagctaattg     3840

gacaccccgt gaaagcatgc tgctggcctt ggcctcgtgt cttttgcaaa ctgcgatctc     3900

cgccttggaa ggcgacctga tggttctcat caatggtttt gctttggcct ggttggcaat     3960

acgagcgatg gttgttccac gcactgataa catcaccttg gcaatcctgg ctgctctgac     4020

accactggcc cggggcacac tgcttgtggc gtggagagca ggccttgcta cttgcggggg     4080

gtttatgctc ctctctctga agggaaaagg cagtgtgaag aagaacttac catttgtcat     4140

ggccctggga ctaaccgctg tgaggctggt cgaccccatc aacgtggtgg gactgctgtt     4200

actcacaagg agtgggaagc ggagctggcc ccctagcgaa gtactcacag ctgttggcct     4260

gatatgcgca ttggctggag ggttcgccaa ggcagatata gagatggctg ggcccatggc     4320

cgcggtcggt ctgctaattg tcagttacgt ggtctcagga aagagtgtgg acatgtacat     4380

tgaaagagca ggtgacatca catgggaaaa agatgcggaa gtcactggaa acagtccccg     4440

gctcgatgtg gcgctagatg agagtggtga tttctccctg gtggaggatg acggtccccc     4500

catgagagag atcatactca aggtggtcct gatgaccatc tgtggcatga atccaatagc     4560

catacccttt gcagctggag cgtggtacgt atacgtgaag actggaaaaa ggagtggtgc     4620

tctatgggat gtgcctgctc ccaaggaagt aaaaaagggg gagaccacag atggagtgta     4680

cagagtaatg actcgtagac tgctaggttc aacacaagtt ggagtgggag ttatgcaaga     4740

gggggtcttt cacactatgt ggcacgtcac aaaaggatcc gcgctgagaa gcggtgaagg     4800

gagacttgat ccatactggg gagatgtcaa gcaggatctg gtgtcatact gtggtccatg     4860

gaagctagat gccgcctggg acgggcacag cgaggtgcag ctcttggccg tgccccccgg     4920

agagagagcg aggaacatcc agactctgcc cggaatattt aagacaaagg atggggacat     4980

tggagcggtt gcgctggatt acccagcagg aacttcagga tctccaatcc tagacaagtg     5040

tgggagagtg ataggacttt atggcaatgg ggtcgtgatc aaaaatggga gttatgttag     5100

tgccatcacc caagggagga gggaagaaga gactcctgtt gagtgcttcg agccctcgat     5160

gctgaagaag aagcagctaa ctgtcttaga cttgcatcct ggagctggga aaaccaggag     5220

agttcttcct gaaatagtcc gtgaagccat aaaaacaaga ctccgtactg tgatcttagc     5280

tccaaccagg gttgtcgctg ctgaaatgga ggaggccctt agagggcttc cagtgcgtta     5340

tatgacaaca gcagtcaatg tcacccactc tggaacagaa atcgtcgact taatgtgcca     5400

tgccaccttc acttcacgtc tactacagcc aatcagagtc cccaactata atctgtatat     5460

tatggatgag gcccacttca cagatccctc aagtatagca gcaagaggat acatttcaac     5520

aagggttgag atgggcgagg cggctgccat cttcatgacc gccacgccac caggaacccg     5580

tgacgcattt ccggactcca actcaccaat tatggacacc gaagtggaag tcccagagag     5640

agcctggagc tcaggctttg attgggtgac ggatcattct ggaaaaacag tttggtttgt     5700

tccaagcgtg aggaacggca atgagatcgc agcttgtctg acaaaggctg gaaaacgggt     5760

catacagctc agcagaaaga cttttgagac agagttccag aaaacaaaac atcaagagtg     5820

ggactttgtc gtgacaactg acatttcaga gatgggcgcc aactttaaag ctgaccgtgt     5880

catagattcc aggagatgcc taaagccggt catacttgat ggcgagagag tcattctggc     5940

tggacccatg cctgtcacac atgccagcgc tgcccagagg agggggcgca taggcaggaa     6000

tcccaacaaa cctggagatg agtatctgta tggaggtggg tgcgcagaga ctgacgaaga     6060

ccatgcacac tggcttgaag caagaatgct ccttgacaat atttacctcc aagatggcct     6120

catagcctcg ctctatcgac ctgaggccga caaagtagca gccattgagg gagagttcaa     6180

gcttaggacg gagcaaagga agacctttgt ggaactcatg aaaagaggag atcttcctgt     6240

ttggctggcc tatcaggttg catctgccgg aataacctac acagatagaa gatggtgctt     6300

tgatggcacg accaacaaca ccataatgga agatagtgtg ccggcagagg tgtggaccag     6360

acacggagag aaaagagtgc tcaaaccgag gtggatggac gccagagttt gttcagatca     6420

tgcggccctg aagtcattca aggagtttgc cgctgggaaa agaggagcgg cttttggagt     6480

gatggaagcc ctgggaacac tgccaggaca catgacagag agattccagg aagccattga     6540

caacctcgct gtgctcatgc gggcagagac tggaagcagg ccttacaaag ccgcggcggc     6600

ccaattgccg gagaccctag agaccataat gcttttgggg ttgctgggaa cagtctcgct     6660

gggaatcttc ttcgtcttga tgaggaacaa gggcataggg aagatgggct ttggaatggt     6720

gactcttggg gccagcgcat ggctcatgtg gctctcggaa attgagccag ccagaattgc     6780

atgtgtcctc attgttgtgt tcctattgct ggtggtgctc atacctgagc cagaaaagca     6840

aagatctccc caggacaacc aaatggcaat catcatcatg gtagcagtag gtcttctggg     6900

cttgattacc gccaatgaac tcggatggtt ggagagaaca aagagtgacc taagccatct     6960

aatgggaagg agagaggagg gggcaaccat aggattctca atggacattg acctgcggcc     7020

agcctcagct tgggccatct atgctgcctt gacaactttc attaccccag ccgtccaaca     7080

tgcagtgacc acttcataca acaactactc cttaatggcg atggccacgc aagctggagt     7140

gttgtttggt atgggcaaag ggatgccatt ctacgcatgg gactttggag tcccgctgct     7200

aatgataggt tgctactcac aattaacacc cctgacccta atagtggcca tcattttgct     7260

cgtggcgcac tacatgtact tgatcccagg gctgcaggca gcagctgcgc gtgctgccca     7320

gaagagaacg gcagctggca tcatgaagaa ccctgttgtg gatggaatag tggtgactga     7380

cattgacaca atgacaattg acccccaagt ggagaaaaag atgggacagg tgctactcat     7440

agcagtagcc gtctccagcg ccatactgtc gcggaccgcc tgggggtggg gggaggctgg     7500

ggccctgatc acagccgcaa cttccacttt gtgggaaggc tctccgaaca agtactggaa     7560

ctcctctaca gccacttcac tgtgtaacat ttttagggga agttacttgg ctggagcttc     7620

tctaatctac acagtaacaa gaaacgctgg cttggtcaag agacgtgggg gtggaacagg     7680

agagaccctg ggagagaaat ggaaggcccg cttgaaccag atgtcggccc tggagttcta     7740

ctcctacaaa aagtcaggca tcaccgaggt gtgcagagaa gaggcccgcc gcgccctcaa     7800

ggacggtgtg gcaacgggag gccatgctgt gtcccgagga agtgcaaagc tgagatggtt     7860

ggtggagcgg ggatacctgc agccctatgg aaaggtcatt gatcttggat gtggcagagg     7920

gggctggagt tactacgccg ccaccatccg caaagttcaa gaagtgaaag gatacacaaa     7980

aggaggccct ggtcatgaag aacccgtgtt ggtgcaaagc tatgggtgga acatagtccg     8040

tcttaagagt ggggtggacg tctttcatat ggcggctgag ccgtgtgaca cgctgctgtg     8100

tgacataggt gagtcatcat ctagtcctga agtggaagaa gcacggacgc tcagagtcct     8160

ctccatggtg ggggattggc ttgaaaaaag accaggagcc ttttgtataa aagtgttgtg     8220

cccatacacc agcactatga tggaaaccct ggagcgactg cagcgtaggt atgggggagg     8280

actggtcaga gtgccactct cccgcaactc tacacatgag atgtactggg tctctggagc     8340

gaaaagcaac accataaaaa gtgtgtccac cacgagccag ctcctcttgg ggcgcatgga     8400

cgggcctagg aggccagtga aatatgagga ggatgtgaat ctcggctctg gcacgcgggc     8460

tgtggtaagc tgcgctgaag ctcccaacat gaagatcatt ggtaaccgca ttgaaaggat     8520

ccgcagtgag cacgcggaaa cgtggttctt tgacgagaac cacccatata ggacatgggc     8580

ttaccatgga agctatgagg cccccacaca agggtcagcg tcctctctaa taaacggggt     8640

tgtcaggctc ctgtcaaaac cctgggatgt ggtgactgga gtcacaggaa tagccatgac     8700

cgacaccaca ccgtatggtc agcaaagagt tttcaaggaa aaagtggaca ctagggtgcc     8760

agacccccaa gaaggcactc gtcaggttat gagcatggtc tcttcctggt tgtggaaaga     8820

gctaggcaaa cacaaacggc cacgagtctg taccaaagaa gagttcatca acaaggttcg     8880

tagcaatgca gcattagggg caatatttga agaggaaaaa gagtggaaga ctgcagtgga     8940

agctgtgaac gatccaaggt tctgggctct agtggacaag gaaagagagc accacctgag     9000

aggagagtgc cagagttgtg tgtataacat gatgggaaaa agagaaaaga aacaagggga     9060

atttggaaag gccaagggca gccgcgccat ctggtatatg tggctagggg ctagatttct     9120

agagttcgaa gcccttggat tcttgaacga ggatcactgg atggggagag agaactcagg     9180

aggtggtgtt gaagggctgg gattacaaag actcggatat gtcctagaag agatgagtcg     9240

tataccagga ggaaggatgt atgcagatga cactgctggc tgggacaccc gcattagcag     9300

gtttgatctg gagaatgaag ctctaatcac caaccaaatg gagaaagggc acagggcctt     9360

ggcattggcc ataatcaagt acacatacca aaacaaagtg gtaaaggtcc ttagaccagc     9420

tgaaaaaggg aaaacagtta tggacattat ttcgagacaa gaccaaaggg ggagcggaca     9480

agttgtcact tacgctctta acacatttac caacctagtg gtgcaactca ttcggaatat     9540

ggaggctgag gaagttctag agatgcaaga cttgtggctg ctgcggaggt cagagaaagt     9600

gaccaactgg ttgcagagca acggatggga taggctcaaa cgaatggcag tcagtggaga     9660

tgattgcgtt gtgaagccaa ttgatgatag gtttgcacat gccctcaggt tcttgaatga     9720

tatgggaaaa gttagaaagg acacacaaga gtggaaaccc tcaactggat gggacaactg     9780

ggaagaagtt ccgttttgct cccaccactt caacaagctc catctcaagg acgggaggtc     9840

cattgtggtt ccctgccgcc accaagatga actgattggc cgggcccgcg tctctccagg     9900

ggcgggatgg agcatccggg agactgcttg cctagcaaaa tcatatgcgc agatgtggca     9960

gctcctttat ttccacagaa gggacctccg actgatggcc aatgccattt gttcatctgt    10020

gccagttgac tgggttccaa ctgggagaac tacctggtca atccatggaa agggagaatg    10080

gatgaccact gaagacatgc ttgtggtgtg gaacagagtg tggattgagg agaacgacca    10140

catggaagac aagaccccag ttacgaaatg gacagacatt ccctatttgg gaaaaaggga    10200

agacttgtgg tgtggatctc tcatagggca cagaccgcgc accacctggg ctgagaacat    10260

taaaaacaca gtcaacatgg tgcgcaggat cataggtgat gaagaaaagt acatggacta    10320

cctatccacc caagttcgct acttgggtga agaagggtct acacctggag tgctgtaagc    10380

accaatttta gtgttgtcag gcctgctagt cagccacagt ttggggaaag ctgtgcagcc    10440

tgtaaccccc ccaggagaag ctgggaaacc aagctcatag tcaggccgag aacgccatgg    10500

cacggaagaa gccatgctgc ctgtgagccc ctcagaggac actgagtcaa aaaaccccac    10560

gcgcttggaa gcgcaggatg ggaaaagaag gtggcgacct tccccaccct tcaatctggg    10620

gcctgaactg gagactagct gtgaatctcc agcagaggga ctagtggtta gaggagaccc    10680

cccggaaaac gcaaaacagc atattgacgc tgggaaagac cagagactcc atgagtttcc    10740

accacgctgg ccgccaggca cagatcgccg aacagcggcg gccggtgtgg ggaaatccat    10800

ggtttct                                                              10807


<210>  5
<211>  3423
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polyprotein sequence the chimeric ZIKALIVax virus (10,807 nt; 
       3,423 aa)

<400>  5

Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met 
1               5                   10                  15      


Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg 
            20                  25                  30          


Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu 
        35                  40                  45              


Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly 
    50                  55                  60                  


Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile 
65                  70                  75                  80  


Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn 
                85                  90                  95      


Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile 
            100                 105                 110         


Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg 
        115                 120                 125             


Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala 
    130                 135                 140                 


Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile 
145                 150                 155                 160 


Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro 
                165                 170                 175     


Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn 
            180                 185                 190         


Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly 
        195                 200                 205             


Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr 
    210                 215                 220                 


Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr 
225                 230                 235                 240 


Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly 
                245                 250                 255     


Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr 
            260                 265                 270         


Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
        275                 280                 285             


Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
    290                 295                 300                 


Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
305                 310                 315                 320 


Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
                325                 330                 335     


Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
            340                 345                 350         


Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
        355                 360                 365             


Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
    370                 375                 380                 


Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
385                 390                 395                 400 


Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
                405                 410                 415     


Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
            420                 425                 430         


Val His Gly Ser Gln His Ser Gly Met Thr Val Asn Asp Ile Gly Tyr 
        435                 440                 445             


Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
    450                 455                 460                 


Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
465                 470                 475                 480 


Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
                485                 490                 495     


Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
            500                 505                 510         


Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
        515                 520                 525             


Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
    530                 535                 540                 


Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
545                 550                 555                 560 


Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
                565                 570                 575     


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
            580                 585                 590         


Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
        595                 600                 605             


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
    610                 615                 620                 


Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
625                 630                 635                 640 


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
                645                 650                 655     


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
            660                 665                 670         


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
        675                 680                 685             


His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
    690                 695                 700                 


Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
705                 710                 715                 720 


Phe Gly Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His 
                725                 730                 735     


Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
            740                 745                 750         


Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn 
        755                 760                 765             


Thr Lys Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val 
    770                 775                 780                 


Met Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val 
785                 790                 795                 800 


Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Ile Tyr 
                805                 810                 815     


Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser 
            820                 825                 830         


Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Glu Gly Ile 
        835                 840                 845             


Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Lys Ser 
    850                 855                 860                 


Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu 
865                 870                 875                 880 


Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln 
                885                 890                 895     


Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp 
            900                 905                 910         


Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val 
        915                 920                 925             


Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Glu His Arg Ala Trp 
    930                 935                 940                 


Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser 
945                 950                 955                 960 


Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala 
                965                 970                 975     


Val Ile Gly Thr Ala Val Lys Gly Arg Glu Ala Ala His Ser Asp Leu 
            980                 985                 990         


Gly Tyr Trp Ile Glu Ser Glu Lys  Asn Asp Thr Trp Arg  Leu Lys Arg 
        995                 1000                 1005             


Ala His  Leu Ile Glu Met Lys  Thr Cys Glu Trp Pro  Lys Ser His 
    1010                 1015                 1020             


Thr Leu  Trp Thr Asp Gly Val  Glu Glu Ser Asp Leu  Ile Ile Pro 
    1025                 1030                 1035             


Lys Ser  Leu Ala Gly Pro Leu  Ser His His Asn Thr  Arg Glu Gly 
    1040                 1045                 1050             


Tyr Arg  Thr Gln Val Lys Gly  Pro Trp His Ser Glu  Glu Leu Glu 
    1055                 1060                 1065             


Ile Arg  Phe Glu Glu Cys Pro  Gly Thr Lys Val Tyr  Val Glu Glu 
    1070                 1075                 1080             


Thr Cys  Gly Thr Arg Gly Pro  Ser Leu Arg Ser Thr  Thr Ala Ser 
    1085                 1090                 1095             


Gly Arg  Val Ile Glu Glu Trp  Cys Cys Arg Glu Cys  Thr Met Pro 
    1100                 1105                 1110             


Pro Leu  Ser Phe Arg Ala Lys  Asp Gly Cys Trp Tyr  Gly Met Glu 
    1115                 1120                 1125             


Ile Arg  Pro Arg Lys Glu Pro  Glu Ser Asn Leu Val  Arg Ser Met 
    1130                 1135                 1140             


Val Thr  Ala Gly Ser Thr Asp  His Met Asp His Phe  Ser Leu Gly 
    1145                 1150                 1155             


Val Leu  Val Ile Leu Leu Met  Val Gln Glu Gly Leu  Lys Lys Arg 
    1160                 1165                 1170             


Met Thr  Thr Lys Ile Ile Met  Ser Thr Ser Met Ala  Val Leu Val 
    1175                 1180                 1185             


Val Met  Ile Leu Gly Gly Phe  Ser Met Ser Asp Leu  Ala Lys Leu 
    1190                 1195                 1200             


Val Ile  Leu Met Gly Ala Thr  Phe Ala Glu Met Asn  Thr Gly Gly 
    1205                 1210                 1215             


Asp Val  Ala His Leu Ala Leu  Val Ala Ala Phe Lys  Val Arg Pro 
    1220                 1225                 1230             


Ala Leu  Leu Val Ser Phe Ile  Phe Arg Ala Asn Trp  Thr Pro Arg 
    1235                 1240                 1245             


Glu Ser  Met Leu Leu Ala Leu  Ala Ser Cys Leu Leu  Gln Thr Ala 
    1250                 1255                 1260             


Ile Ser  Ala Leu Glu Gly Asp  Leu Met Val Leu Ile  Asn Gly Phe 
    1265                 1270                 1275             


Ala Leu  Ala Trp Leu Ala Ile  Arg Ala Met Ala Val  Pro Arg Thr 
    1280                 1285                 1290             


Asp Asn  Ile Ala Leu Pro Ile  Leu Ala Ala Leu Thr  Pro Leu Ala 
    1295                 1300                 1305             


Arg Gly  Thr Leu Leu Val Ala  Trp Arg Ala Gly Leu  Ala Thr Cys 
    1310                 1315                 1320             


Gly Gly  Ile Met Leu Leu Ser  Leu Lys Gly Lys Gly  Ser Val Lys 
    1325                 1330                 1335             


Lys Asn  Leu Pro Phe Val Met  Ala Leu Gly Leu Thr  Ala Val Arg 
    1340                 1345                 1350             


Val Val  Asp Pro Ile Asn Val  Val Gly Leu Leu Leu  Leu Thr Arg 
    1355                 1360                 1365             


Ser Gly  Lys Arg Ser Trp Pro  Pro Ser Glu Val Leu  Thr Ala Val 
    1370                 1375                 1380             


Gly Leu  Ile Cys Ala Leu Ala  Gly Gly Phe Ala Lys  Ala Asp Ile 
    1385                 1390                 1395             


Glu Met  Ala Gly Pro Met Ala  Ala Val Gly Leu Leu  Ile Val Ser 
    1400                 1405                 1410             


Tyr Val  Val Ser Gly Lys Ser  Val Asp Met Tyr Ile  Glu Arg Ala 
    1415                 1420                 1425             


Gly Asp  Ile Thr Trp Glu Lys  Asp Ala Glu Val Thr  Gly Asn Ser 
    1430                 1435                 1440             


Pro Arg  Leu Asp Val Ala Leu  Asp Glu Ser Gly Asp  Phe Ser Leu 
    1445                 1450                 1455             


Val Glu  Glu Asp Gly Pro Pro  Met Arg Glu Ile Ile  Leu Lys Val 
    1460                 1465                 1470             


Val Leu  Met Ala Ile Cys Gly  Met Asn Pro Ile Ala  Ile Pro Phe 
    1475                 1480                 1485             


Ala Ala  Gly Ala Trp Tyr Val  Tyr Val Lys Thr Gly  Lys Arg Ser 
    1490                 1495                 1500             


Gly Ala  Leu Trp Asp Val Pro  Ala Pro Lys Glu Val  Lys Lys Gly 
    1505                 1510                 1515             


Glu Thr  Thr Asp Gly Val Tyr  Arg Val Met Thr Arg  Arg Leu Leu 
    1520                 1525                 1530             


Gly Ser  Thr Gln Val Gly Val  Gly Val Met Gln Glu  Gly Val Phe 
    1535                 1540                 1545             


His Thr  Met Trp His Val Thr  Lys Gly Ala Ala Leu  Arg Ser Gly 
    1550                 1555                 1560             


Glu Gly  Arg Leu Asp Pro Tyr  Trp Gly Asp Val Lys  Gln Asp Leu 
    1565                 1570                 1575             


Val Ser  Tyr Cys Gly Pro Trp  Lys Leu Asp Ala Ala  Trp Asp Gly 
    1580                 1585                 1590             


Leu Ser  Glu Val Gln Leu Leu  Ala Val Pro Pro Gly  Glu Arg Ala 
    1595                 1600                 1605             


Arg Asn  Ile Gln Thr Leu Pro  Gly Ile Phe Lys Thr  Lys Asp Gly 
    1610                 1615                 1620             


Asp Ile  Gly Ala Val Ala Leu  Asp Tyr Pro Ala Gly  Thr Ser Gly 
    1625                 1630                 1635             


Ser Pro  Ile Leu Asp Lys Cys  Gly Arg Val Ile Gly  Leu Tyr Gly 
    1640                 1645                 1650             


Asn Gly  Val Val Ile Lys Asn  Gly Ser Tyr Val Ser  Ala Ile Thr 
    1655                 1660                 1665             


Gln Gly  Lys Arg Glu Glu Glu  Thr Pro Val Glu Cys  Phe Glu Pro 
    1670                 1675                 1680             


Ser Met  Leu Lys Lys Lys Gln  Leu Thr Val Leu Asp  Leu His Pro 
    1685                 1690                 1695             


Gly Ala  Gly Lys Thr Arg Arg  Val Leu Pro Glu Ile  Val Arg Glu 
    1700                 1705                 1710             


Ala Ile  Lys Lys Arg Leu Arg  Thr Val Ile Leu Ala  Pro Thr Arg 
    1715                 1720                 1725             


Val Val  Ala Ala Glu Met Glu  Glu Ala Leu Arg Gly  Leu Pro Val 
    1730                 1735                 1740             


Arg Tyr  Met Thr Thr Ala Val  Asn Val Thr His Ser  Gly Thr Glu 
    1745                 1750                 1755             


Ile Val  Asp Leu Met Cys His  Ala Thr Phe Thr Ser  Arg Leu Leu 
    1760                 1765                 1770             


Gln Pro  Ile Arg Val Pro Asn  Tyr Asn Leu Tyr Ile  Met Asp Glu 
    1775                 1780                 1785             


Ala His  Phe Thr Asp Pro Ser  Ser Ile Ala Ala Arg  Gly Tyr Ile 
    1790                 1795                 1800             


Ser Thr  Arg Val Glu Met Gly  Glu Ala Ala Ala Ile  Phe Met Thr 
    1805                 1810                 1815             


Ala Thr  Pro Pro Gly Thr Arg  Asp Ala Phe Pro Asp  Ser Asn Ser 
    1820                 1825                 1830             


Pro Ile  Met Asp Thr Glu Val  Glu Val Pro Glu Arg  Ala Trp Ser 
    1835                 1840                 1845             


Ser Gly  Phe Asp Trp Val Thr  Asp His Ser Gly Lys  Thr Val Trp 
    1850                 1855                 1860             


Phe Val  Pro Ser Val Arg Asn  Gly Asn Glu Ile Ala  Ala Cys Leu 
    1865                 1870                 1875             


Thr Lys  Ala Gly Lys Arg Val  Ile Gln Leu Ser Arg  Lys Thr Phe 
    1880                 1885                 1890             


Glu Thr  Glu Phe Gln Lys Thr  Lys Asn Gln Glu Trp  Asp Phe Val 
    1895                 1900                 1905             


Ile Thr  Thr Asp Ile Ser Glu  Met Gly Ala Asn Phe  Lys Ala Asp 
    1910                 1915                 1920             


Arg Val  Ile Asp Ser Arg Arg  Cys Leu Lys Pro Val  Ile Leu Asp 
    1925                 1930                 1935             


Gly Glu  Arg Val Ile Leu Ala  Gly Pro Met Pro Val  Thr His Ala 
    1940                 1945                 1950             


Ser Ala  Ala Gln Arg Arg Gly  Arg Ile Gly Arg Asn  Pro Asn Lys 
    1955                 1960                 1965             


Pro Gly  Asp Glu Tyr Met Tyr  Gly Gly Gly Cys Ala  Glu Thr Asp 
    1970                 1975                 1980             


Glu Gly  His Ala His Trp Leu  Glu Ala Arg Met Leu  Leu Asp Asn 
    1985                 1990                 1995             


Ile Tyr  Leu Gln Asp Gly Leu  Ile Ala Ser Leu Tyr  Arg Pro Glu 
    2000                 2005                 2010             


Ala Asp  Lys Val Ala Ala Ile  Glu Gly Glu Phe Lys  Leu Arg Thr 
    2015                 2020                 2025             


Glu Gln  Arg Lys Thr Phe Val  Glu Leu Met Lys Arg  Gly Asp Leu 
    2030                 2035                 2040             


Pro Val  Trp Leu Ala Tyr Gln  Val Ala Ser Ala Gly  Ile Thr Tyr 
    2045                 2050                 2055             


Thr Asp  Arg Arg Trp Cys Phe  Asp Gly Thr Thr Asn  Asn Thr Ile 
    2060                 2065                 2070             


Met Glu  Asp Ser Val Pro Ala  Glu Val Trp Thr Lys  Tyr Gly Glu 
    2075                 2080                 2085             


Lys Arg  Val Leu Lys Pro Arg  Trp Met Asp Ala Arg  Val Cys Ser 
    2090                 2095                 2100             


Asp His  Ala Ala Leu Lys Ser  Phe Lys Glu Phe Ala  Ala Gly Lys 
    2105                 2110                 2115             


Arg Gly  Ala Ala Leu Gly Val  Met Glu Ala Leu Gly  Thr Leu Pro 
    2120                 2125                 2130             


Gly His  Met Thr Glu Arg Phe  Gln Glu Ala Ile Asp  Asn Leu Ala 
    2135                 2140                 2145             


Val Leu  Met Arg Ala Glu Thr  Gly Ser Arg Pro Tyr  Lys Ala Ala 
    2150                 2155                 2160             


Ala Ala  Gln Leu Pro Glu Thr  Leu Glu Thr Ile Met  Leu Leu Gly 
    2165                 2170                 2175             


Leu Leu  Gly Thr Val Ser Leu  Gly Ile Phe Phe Val  Leu Met Arg 
    2180                 2185                 2190             


Asn Lys  Gly Ile Gly Lys Met  Gly Phe Gly Met Val  Thr Leu Gly 
    2195                 2200                 2205             


Ala Ser  Ala Trp Leu Met Trp  Leu Ser Glu Ile Glu  Pro Ala Arg 
    2210                 2215                 2220             


Ile Ala  Cys Val Leu Ile Val  Val Phe Leu Leu Leu  Val Val Leu 
    2225                 2230                 2235             


Ile Pro  Glu Pro Glu Lys Gln  Arg Ser Pro Gln Asp  Asn Gln Met 
    2240                 2245                 2250             


Ala Ile  Ile Ile Met Val Ala  Val Gly Leu Leu Gly  Leu Ile Thr 
    2255                 2260                 2265             


Ala Asn  Glu Leu Gly Trp Leu  Glu Arg Thr Lys Asn  Asp Ile Ala 
    2270                 2275                 2280             


His Leu  Met Gly Arg Arg Glu  Glu Gly Ala Thr Met  Gly Phe Ser 
    2285                 2290                 2295             


Met Asp  Ile Asp Leu Arg Pro  Ala Ser Ala Trp Ala  Ile Tyr Ala 
    2300                 2305                 2310             


Ala Leu  Thr Thr Leu Ile Thr  Pro Ala Val Gln His  Ala Val Thr 
    2315                 2320                 2325             


Thr Ser  Tyr Asn Asn Tyr Ser  Leu Met Ala Met Ala  Thr Gln Ala 
    2330                 2335                 2340             


Gly Val  Leu Phe Gly Met Gly  Lys Gly Met Pro Phe  Tyr Ala Trp 
    2345                 2350                 2355             


Asp Leu  Gly Val Pro Leu Leu  Met Met Gly Cys Tyr  Ser Gln Leu 
    2360                 2365                 2370             


Thr Pro  Leu Thr Leu Ile Val  Ala Ile Ile Leu Leu  Val Ala His 
    2375                 2380                 2385             


Tyr Met  Tyr Leu Ile Pro Gly  Leu Gln Ala Ala Ala  Ala Arg Ala 
    2390                 2395                 2400             


Ala Gln  Lys Arg Thr Ala Ala  Gly Ile Met Lys Asn  Pro Val Val 
    2405                 2410                 2415             


Asp Gly  Ile Val Val Thr Asp  Ile Asp Thr Met Thr  Ile Asp Pro 
    2420                 2425                 2430             


Gln Val  Glu Lys Lys Met Gly  Gln Val Leu Leu Ile  Ala Val Ala 
    2435                 2440                 2445             


Ile Ser  Ser Ala Val Leu Leu  Arg Thr Ala Trp Gly  Trp Gly Glu 
    2450                 2455                 2460             


Ala Gly  Ala Leu Ile Thr Ala  Ala Thr Ser Thr Leu  Trp Glu Gly 
    2465                 2470                 2475             


Ser Pro  Asn Lys Tyr Trp Asn  Ser Ser Thr Ala Thr  Ser Leu Cys 
    2480                 2485                 2490             


Asn Ile  Phe Arg Gly Ser Tyr  Leu Ala Gly Ala Ser  Leu Ile Tyr 
    2495                 2500                 2505             


Thr Val  Thr Arg Asn Ala Gly  Leu Val Lys Arg Arg  Gly Gly Gly 
    2510                 2515                 2520             


Thr Gly  Glu Thr Leu Gly Glu  Lys Trp Lys Ala Arg  Leu Asn Gln 
    2525                 2530                 2535             


Met Ser  Ala Leu Glu Phe Tyr  Ser Tyr Lys Lys Ser  Gly Ile Thr 
    2540                 2545                 2550             


Glu Val  Cys Arg Glu Glu Ala  Arg Arg Ala Leu Lys  Asp Gly Val 
    2555                 2560                 2565             


Ala Thr  Gly Gly His Ala Val  Ser Arg Gly Ser Ala  Lys Leu Arg 
    2570                 2575                 2580             


Trp Leu  Val Glu Arg Gly Tyr  Leu Gln Pro Tyr Gly  Lys Val Val 
    2585                 2590                 2595             


Asp Leu  Gly Cys Gly Arg Gly  Gly Trp Ser Tyr Tyr  Ala Ala Thr 
    2600                 2605                 2610             


Ile Arg  Lys Val Gln Glu Val  Arg Gly Tyr Thr Lys  Gly Gly Pro 
    2615                 2620                 2625             


Gly His  Glu Glu Pro Met Leu  Val Gln Ser Tyr Gly  Trp Asn Ile 
    2630                 2635                 2640             


Val Arg  Leu Lys Ser Gly Val  Asp Val Phe His Met  Ala Ala Glu 
    2645                 2650                 2655             


Pro Cys  Asp Thr Leu Leu Cys  Asp Ile Gly Glu Ser  Ser Ser Ser 
    2660                 2665                 2670             


Pro Glu  Val Glu Glu Thr Arg  Thr Leu Arg Val Leu  Ser Met Val 
    2675                 2680                 2685             


Gly Asp  Trp Leu Glu Lys Arg  Pro Gly Ala Phe Cys  Ile Lys Val 
    2690                 2695                 2700             


Leu Cys  Pro Tyr Thr Ser Thr  Met Met Glu Thr Met  Glu Arg Leu 
    2705                 2710                 2715             


Gln Arg  Arg His Gly Gly Gly  Leu Val Arg Val Pro  Leu Ser Arg 
    2720                 2725                 2730             


Asn Ser  Thr His Glu Met Tyr  Trp Val Ser Gly Ala  Lys Ser Asn 
    2735                 2740                 2745             


Ile Ile  Lys Ser Val Ser Thr  Thr Ser Gln Leu Leu  Leu Gly Arg 
    2750                 2755                 2760             


Met Asp  Gly Pro Arg Arg Pro  Val Lys Tyr Glu Glu  Asp Val Asn 
    2765                 2770                 2775             


Leu Gly  Ser Gly Thr Arg Ala  Val Ala Ser Cys Ala  Glu Ala Pro 
    2780                 2785                 2790             


Asn Met  Lys Ile Ile Gly Arg  Arg Ile Glu Arg Ile  Arg Asn Glu 
    2795                 2800                 2805             


His Ala  Glu Thr Trp Phe Leu  Asp Glu Asn His Pro  Tyr Arg Thr 
    2810                 2815                 2820             


Trp Ala  Tyr His Gly Ser Tyr  Glu Ala Pro Thr Gln  Gly Ser Ala 
    2825                 2830                 2835             


Ser Ser  Leu Val Asn Gly Val  Val Arg Leu Leu Ser  Lys Pro Trp 
    2840                 2845                 2850             


Asp Val  Val Thr Gly Val Thr  Gly Ile Ala Met Thr  Asp Thr Thr 
    2855                 2860                 2865             


Pro Tyr  Gly Gln Gln Arg Val  Phe Lys Glu Lys Val  Asp Thr Arg 
    2870                 2875                 2880             


Val Pro  Asp Pro Gln Glu Gly  Thr Arg Gln Val Met  Asn Ile Val 
    2885                 2890                 2895             


Ser Ser  Trp Leu Trp Lys Glu  Leu Gly Lys Arg Lys  Arg Pro Arg 
    2900                 2905                 2910             


Val Cys  Thr Lys Glu Glu Phe  Ile Asn Lys Val Arg  Ser Asn Ala 
    2915                 2920                 2925             


Ala Leu  Gly Ala Ile Phe Glu  Glu Glu Lys Glu Trp  Lys Thr Ala 
    2930                 2935                 2940             


Val Glu  Ala Val Asn Asp Pro  Arg Phe Trp Ala Leu  Val Asp Arg 
    2945                 2950                 2955             


Glu Arg  Glu His His Leu Arg  Gly Glu Cys His Ser  Cys Val Tyr 
    2960                 2965                 2970             


Asn Met  Met Gly Lys Arg Glu  Lys Lys Gln Gly Glu  Phe Gly Lys 
    2975                 2980                 2985             


Ala Lys  Gly Ser Arg Ala Ile  Trp Tyr Met Trp Leu  Gly Ala Arg 
    2990                 2995                 3000             


Phe Leu  Glu Phe Glu Ala Leu  Gly Phe Leu Asn Glu  Asp His Trp 
    3005                 3010                 3015             


Met Gly  Arg Glu Asn Ser Gly  Gly Gly Val Glu Gly  Leu Gly Leu 
    3020                 3025                 3030             


Gln Arg  Leu Gly Tyr Ile Leu  Glu Glu Met Asn Arg  Ala Pro Gly 
    3035                 3040                 3045             


Gly Lys  Met Tyr Ala Asp Asp  Thr Ala Gly Trp Asp  Thr Arg Ile 
    3050                 3055                 3060             


Ser Lys  Phe Asp Leu Glu Asn  Glu Ala Leu Ile Thr  Asn Gln Met 
    3065                 3070                 3075             


Glu Glu  Gly His Arg Thr Leu  Ala Leu Ala Val Ile  Lys Tyr Thr 
    3080                 3085                 3090             


Tyr Gln  Asn Lys Val Val Lys  Val Leu Arg Pro Ala  Glu Gly Gly 
    3095                 3100                 3105             


Lys Thr  Val Met Asp Ile Ile  Ser Arg Gln Asp Gln  Arg Gly Ser 
    3110                 3115                 3120             


Gly Gln  Val Val Thr Tyr Ala  Leu Asn Thr Phe Thr  Asn Leu Val 
    3125                 3130                 3135             


Val Gln  Leu Ile Arg Asn Met  Glu Ala Glu Glu Val  Leu Glu Met 
    3140                 3145                 3150             


Gln Asp  Leu Trp Leu Leu Arg  Lys Pro Glu Lys Val  Thr Arg Trp 
    3155                 3160                 3165             


Leu Gln  Ser Asn Gly Trp Asp  Arg Leu Lys Arg Met  Ala Val Ser 
    3170                 3175                 3180             


Gly Asp  Asp Cys Val Val Lys  Pro Ile Asp Asp Arg  Phe Ala His 
    3185                 3190                 3195             


Ala Leu  Arg Phe Leu Asn Asp  Met Gly Lys Val Arg  Lys Asp Thr 
    3200                 3205                 3210             


Gln Glu  Trp Lys Pro Ser Thr  Gly Trp Ser Asn Trp  Glu Glu Val 
    3215                 3220                 3225             


Pro Phe  Cys Ser His His Phe  Asn Lys Leu Tyr Leu  Lys Asp Gly 
    3230                 3235                 3240             


Arg Ser  Ile Val Val Pro Cys  Arg His Gln Asp Glu  Leu Ile Gly 
    3245                 3250                 3255             


Arg Ala  Arg Val Ser Pro Gly  Ala Gly Trp Ser Ile  Arg Glu Thr 
    3260                 3265                 3270             


Ala Cys  Leu Ala Lys Ser Tyr  Ala Gln Met Trp Gln  Leu Leu Tyr 
    3275                 3280                 3285             


Phe His  Arg Arg Asp Leu Arg  Leu Met Ala Asn Ala  Ile Cys Ser 
    3290                 3295                 3300             


Ala Val  Pro Val Asp Trp Val  Pro Thr Gly Arg Thr  Thr Trp Ser 
    3305                 3310                 3315             


Ile His  Gly Lys Gly Glu Trp  Met Thr Thr Glu Asp  Met Leu Met 
    3320                 3325                 3330             


Val Trp  Asn Arg Val Trp Ile  Glu Glu Asn Asp His  Met Glu Asp 
    3335                 3340                 3345             


Lys Thr  Pro Val Thr Lys Trp  Thr Asp Ile Pro Tyr  Leu Gly Lys 
    3350                 3355                 3360             


Arg Glu  Asp Leu Trp Cys Gly  Ser Leu Ile Gly His  Arg Pro Arg 
    3365                 3370                 3375             


Thr Thr  Trp Ala Glu Asn Ile  Lys Asp Thr Val Asn  Met Val Arg 
    3380                 3385                 3390             


Arg Ile  Ile Gly Asp Glu Glu  Lys Tyr Met Asp Tyr  Leu Ser Thr 
    3395                 3400                 3405             


Gln Val  Arg Tyr Leu Gly Glu  Glu Gly Ser Thr Pro  Gly Val Leu 
    3410                 3415                 3420             


<210>  6
<211>  10807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genomic sequence of the chimeric ZIKALIVax virus (10,807 nt; 
       3,423 aa):

<400>  6
agttgttgat ctgtgtgagt cagactgcga cagttcgagt ctgaagcgag agctaacaac       60

agtatcaaca ggtttaattt ggatttggaa acgagagttt ctggtcatga aaaacccaaa      120

aaagaaatcc ggaggattcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtgag      180

cccctttggg ggcttgaaga ggctgccagc cggacttctg ctgggtcatg ggcccatcag      240

gatggtcttg gcgattctag cctttttgag attcacggca atcaagccat cactgggtct      300

catcaataga tggggttcag ttgggaaaaa agaggctatg gaaataataa agaagttcaa      360

gaaagatctg gctgccatgc tgagaataat caatgctagg aaggagaaga agagacgagg      420

cgcagatact agtgtcggaa ttgttggcct cctgctgacc acagctatgg cagcggaggt      480

cactagacgt gggagtgcat actatatgta cttggacaga aacgatgctg gggaggccat      540

atcttttcca accacattgg ggatgaataa gtgttatata cagatcatgg atcttggaca      600

catgtgtgat gccaccatga gctatgaatg ccctatgctg gatgaggggg tggaaccaga      660

tgacgtcgat tgttggtgca acacgacgtc aacttgggtt gtgtacggaa cctgccatca      720

caaaaaaggt gaagcacgga gatctagaag agctgtgacg ctcccctccc attccactag      780

gaagctgcaa acgcggtcgc aaacctggtt ggaatcaaga gaatacacaa agcacttgat      840

tagagtcgaa aattggatat tcaggaaccc tggcttcgcg ttagcagcag ctgccatcgc      900

ttggcttttg ggaagctcaa cgagccaaaa agtcatatac ttggtcatga tactgctgat      960

tgccccggca tacagcatca ggtgcatagg agtcagcaat agggactttg tggaaggtat     1020

gtcaggtggg acttgggttg atgttgtctt ggaacatgga ggttgtgtca ccgtaatggc     1080

acaggacaaa ccgactgtcg acatagagct ggttacaaca acagtcagca acatggcgga     1140

ggtacgatcg tactgctatg aggcatcaat atcagacatg gcttcggaca gccgctgccc     1200

aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac     1260

gttagtggac agaggctggg gaaatggatg tggacttttt ggcaaaggga gcctggtgac     1320

atgcgctaag tttgcatgct ccaagaaaat gaccgggaag agcatccagc cagagaatct     1380

ggagtaccgg ataatgctgt cagttcatgg ctcccagcac agtgggatga ctgtcaatga     1440

tataggatat gaaactgatg agaatagagc gaaagttgag ataacgccca attcaccaag     1500

agccgaagcc accctggggg gttttggaag cctaggactt gattgtgaac cgaggacagg     1560

ccttgacttt tcagatttgt attacttgac tatgaataac aagcactggt tggttcacaa     1620

ggagtggttc cacgacattc cattaccttg gcacgctggg gcagacaccg gaactccaca     1680

ctggaacaac aaagaagcac tggtagagtt caaggacgca catgccaaaa ggcaaactgt     1740

cgtggttcta gggagtcaag aaggagcagt tcacacggcc cttgctggag ctctggaggc     1800

tgagatggat ggtgcaaagg gaaggctgtc ctctggccac ttgaaatgtc gcctgaaaat     1860

ggataaactt agattgaagg gcgtgtcata ctccttgtgt actgcagcgt tcacattcac     1920

caagatcccg gctgaaacac tgcacgggac agtcacagtg gaggtacagt acgcagggac     1980

agatggacct tgcaaggttc cagctcagat ggcggtggac atgcaaactc tgaccccagt     2040

tgggaggttg ataaccgcta accccgtaat cactgaaagc actgagaact ctaagatgat     2100

gctggaactt gatccaccat ttggggactc ttacattgtc ataggagtcg gggagaagaa     2160

gatcacccac cactggcaca ggagtggcag caccattgga aaagcatttg aggccactgt     2220

gagaggcgcc aagagaatgg cagtcctggg ggatacagcc tgggacttcg gatcagtcgg     2280

gggtgtgttc aactcactgg gtaagggcat tcaccagatt tttggagcag ccttcaaatc     2340

actgtttgga ggaatgtcct ggttctcaca gatcctcata ggcacgctgc tagtgtggtt     2400

aggtttgaac acaaagaatg gatctatctc cctcacatgc ttggccctgg ggggagtgat     2460

gatcttcctc tccacggctg tttctgctga cgtggggtgc tcagtggact tctcaaaaaa     2520

ggaaacgaga tgtggcacgg gggtattcat ctataatgat gttgaagcct ggagggaccg     2580

gtacaagtac catcctgact ccccccgcag attggcagca gcagtcaagc aggcctggga     2640

agaggggatc tgtgggatct catccgtttc aagaatggaa aacatcatgt ggaaatcagt     2700

agaaggggag ctcaatgcta tcctagagga gaatggagtt caactgacag ttgttgtggg     2760

atctgtaaaa aaccccatgt ggagaggtcc acaaagattg ccagtgcctg tgaatgagct     2820

gccccatggc tggaaagcct gggggaaatc gtattttgtt agggcggcaa agaccaacaa     2880

cagttttgtt gtcgacggtg acacactgaa ggaatgtccg cttgagcaca gagcatggaa     2940

tagttttctt gtggaggatc acgggtttgg agtcttccac accagtgtct ggcttaaggt     3000

cagagaagat tactcattag aatgtgaccc agccgtcata ggaacagctg ttaagggaag     3060

ggaggccgcg cacagtgatc tgggctattg gattgaaagt gaaaagaatg acacatggag     3120

gctgaagagg gcccacctga ttgagatgaa aacatgtgaa tggccaaagt ctcacacatt     3180

gtggacagat ggagtagaag aaagtgatct tatcataccc aagtctttag ctggtccact     3240

cagccaccac aacaccagag agggttacag aacccaagtg aaagggccat ggcacagtga     3300

agagcttgaa atccggtttg aggaatgtcc aggcaccaag gtttacgtgg aggagacatg     3360

cggaactaga ggaccatctc tgagatcaac tactgcaagt ggaagggtca ttgaggaatg     3420

gtgctgtagg gaatgcacaa tgcccccact atcgtttcga gcaaaagacg gctgctggta     3480

tggaatggag ataaggccca ggaaagaacc agagagcaac ttagtgaggt caatggtgac     3540

agcggggtca accgatcata tggaccactt ctctcttgga gtgcttgtga ttctactcat     3600

ggtgcaggag gggttgaaga agagaatgac cacaaagatc atcatgagca catcaatggc     3660

agtgctggta gtcatgatct tgggaggatt ttcaatgagt gacctggcca agcttgtgat     3720

cctgatgggt gctactttcg cagaaatgaa cactggagga gatgtagctc acttggcatt     3780

ggtagcggca tttaaagtca gaccagcctt gctggtctcc ttcattttca gagccaattg     3840

gacaccccgt gagagcatgc tgctagccct ggcttcgtgt cttctgcaaa ctgcgatctc     3900

tgctcttgaa ggtgacttga tggtcctcat taatggattt gctttggcct ggttggcaat     3960

tcgagcaatg gccgtgccac gcactgacaa catcgctcta ccaatcttgg ctgctctaac     4020

accactagct cgaggcacac tgctcgtggc atggagagcg ggcctggcta cttgtggagg     4080

gatcatgctc ctctccctga aagggaaagg tagtgtgaag aagaacctgc catttgtcat     4140

ggccctggga ttgacagctg tgagggtagt agaccctatt aatgtggtag gactactgtt     4200

actcacaagg agtgggaagc ggagctggcc ccctagtgaa gttctcacag ccgttggcct     4260

gatatgtgca ctggccggag ggtttgccaa ggcagacatt gagatggctg gacccatggc     4320

tgcagtaggc ttgctaattg tcagctatgt ggtctcggga aagagtgtgg acatgtacat     4380

tgaaagagca ggtgacatca catgggaaaa ggacgcggaa gtcactggaa acagtcctcg     4440

gcttgacgtg gcactggatg agagtggtga tttctccttg gtagaggaag atggtccacc     4500

catgagagag atcatactta aggtggtcct gatggccatc tgtggcatga acccaatagc     4560

tatacctttt gctgcaggag cgtggtatgt gtatgtgaag actgggaaaa ggagtggcgc     4620

cctctgggac gtgcctgctc ccaaagaagt gaagaaagga gagaccacag atggagtgta     4680

cagagtgatg actcgcagac tgctaggttc aacacaggtt ggagtgggag tcatgcaaga     4740

gggagtcttc cacaccatgt ggcacgttac aaaaggagcc gcactgagga gcggtgaggg     4800

aagacttgat ccatactggg gggatgtcaa gcaggacttg gtgtcatact gtgggccttg     4860

gaagttggat gcagcttggg atggactcag cgaggtacag cttttggccg tacctcccgg     4920

agagagggcc agaaacattc agaccctgcc tggaatattc aagacaaagg acggggacat     4980

cggagcagtt gctctggact accctgcagg gacctcagga tctccgatcc tagacaaatg     5040

tggaagagtg ataggactct atggcaatgg ggttgtgatc aagaatggaa gctatgttag     5100

tgctataacc cagggaaaga gggaggagga gactccggtt gaatgtttcg aaccctcgat     5160

gctgaagaag aagcagctaa ctgtcttgga tctgcatcca ggagccggaa aaaccaggag     5220

agttcttcct gaaatagtcc gtgaagccat aaaaaagaga ctccggacag tgatcttggc     5280

accaactagg gttgtcgctg ctgagatgga ggaggccttg agaggacttc cggtgcgtta     5340

catgacaaca gcagtcaacg tcacccattc tgggacagaa atcgttgatt tgatgtgcca     5400

tgccactttc acttcacgct tactacaacc catcagagtc cctaattaca atctctacat     5460

catggatgaa gcccacttca cagacccctc aagtatagct gcaagaggat atatatcaac     5520

aagggttgaa atgggcgagg cggctgccat ttttatgact gccacaccac caggaacccg     5580

tgatgcgttt cctgactcta actcaccaat catggacaca gaagtggaag tcccagagag     5640

agcctggagc tcaggctttg attgggtgac agaccattct gggaaaacag tttggttcgt     5700

tccaagcgtg agaaacggaa atgaaatcgc agcctgtctg acaaaggctg gaaagcgggt     5760

catacagctc agcaggaaga cttttgagac agaatttcag aaaacaaaaa atcaagagtg     5820

ggactttgtc ataacaactg acatctcaga gatgggcgcc aacttcaagg ctgaccgggt     5880

catagactct aggagatgcc taaaaccagt catacttgat ggtgagagag tcatcttggc     5940

tgggcccatg cctgtcacgc atgctagtgc tgctcagagg agaggacgta taggcaggaa     6000

ccctaacaaa cctggagatg agtacatgta tggaggtggg tgtgcagaga ctgatgaagg     6060

ccatgcacac tggcttgaag caagaatgct tcttgacaac atctacctcc aggatggcct     6120

catagcctcg ctctatcggc ctgaggccga taaggtagcc gccattgagg gagagtttaa     6180

gctgaggaca gagcaaagga agaccttcgt ggaactcatg aagagaggag accttcccgt     6240

ctggctagcc tatcaggttg catctgccgg aataacttac acagacagaa gatggtgctt     6300

tgatggcaca accaacaaca ccataatgga agacagcgta ccagcagagg tgtggacaaa     6360

gtatggagag aagagagtgc tcaaaccgag atggatggat gctagggtct gttcagacca     6420

tgcggccctg aagtcgttca aagaattcgc cgctggaaaa agaggagcgg ctttgggagt     6480

aatggaggcc ctgggaacac tgccaggaca catgacagag aggtttcagg aagccattga     6540

caacctcgcc gtgctcatgc gagcagagac tggaagcagg ccttataagg cagcggcagc     6600

ccaactgccg gagaccctag agaccattat gctcttaggt ttgctgggaa cagtttcact     6660

ggggatcttc ttcgtcttga tgcggaataa gggcatcggg aagatgggct ttggaatggt     6720

aacccttggg gccagtgcat ggctcatgtg gctttcggaa attgaaccag ccagaattgc     6780

atgtgtcctc attgttgtgt ttttattact ggtggtgctc atacccgagc cagagaagca     6840

aagatctccc caagataacc agatggcaat tatcatcatg gtggcagtgg gccttctagg     6900

tttgataact gcaaacgaac ttggatggct ggaaagaaca aaaaatgaca tagctcatct     6960

aatgggaagg agagaagaag gagcaaccat gggattctca atggacattg atctgcggcc     7020

agcctccgcc tgggctatct atgccgcatt gacaactctc atcaccccag ctgtccaaca     7080

tgcggtaacc acttcataca acaactactc cttaatggcg atggccacac aagctggagt     7140

gctgtttggc atgggcaaag ggatgccatt ttatgcatgg gaccttggag tcccgctgct     7200

aatgatgggt tgctattcac aattaacacc cctgactctg atagtagcta tcattctgct     7260

tgtggcgcac tacatgtact tgatcccagg cctacaagcg gcagcagcgc gtgctgccca     7320

gaaaaggaca gcagctggca tcatgaagaa tcccgttgtg gatggaatag tggtaactga     7380

cattgacaca atgacaatag acccccaggt ggagaagaag atgggacaag tgttactcat     7440

agcagtagcc atctccagtg ctgtgctgct gcggaccgcc tggggatggg gggaggctgg     7500

agctctgatc acagcagcga cctccacctt gtgggaaggc tctccaaaca aatactggaa     7560

ctcctctaca gccacctcac tgtgcaacat cttcagagga agctatctgg caggagcttc     7620

ccttatctat acagtgacga gaaacgctgg cctggttaag agacgtggag gtgggacggg     7680

agagactctg ggagagaagt ggaaagctcg tctgaatcag atgtcggccc tggagttcta     7740

ctcttataaa aagtcaggta tcactgaagt gtgtagagag gaggctcgcc gtgccctcaa     7800

ggatggagtg gccacaggag gacatgccgt atcccgggga agtgcaaagc tcagatggtt     7860

ggtggagaga ggatatctgc agccctatgg gaaggttgtt gacctcggat gtggcagagg     7920

gggctggagc tattatgccg ccaccatccg caaagtgcag gaggtgagag gatacacaaa     7980

gggaggtccc ggtcatgaag aacccatgct ggtgcaaagc tatgggtgga acatagttcg     8040

tctcaagagt ggagtggacg tcttccacat ggcggctgag ccgtgtgaca ctctgctgtg     8100

tgacataggt gagtcatcat ctagtcctga agtggaagag acacgaacac tcagagtgct     8160

ctctatggtg ggggactggc ttgaaaaaag accaggggcc ttctgtataa aggtgctgtg     8220

cccatacacc agcactatga tggaaaccat ggagcgactg caacgtaggc atgggggagg     8280

attagtcaga gtgccattgt ctcgcaactc cacacatgag atgtactggg tctctggggc     8340

aaagagcaac atcataaaaa gtgtgtccac cacaagtcag ctcctcctgg gacgcatgga     8400

tggccccagg aggccagtga aatatgagga ggatgtgaac ctcggctcgg gtacacgagc     8460

tgtggcaagc tgtgctgagg ctcctaacat gaaaatcatc ggcaggcgca ttgagagaat     8520

ccgcaatgaa catgcagaaa catggtttct tgatgaaaac cacccataca ggacatgggc     8580

ctaccatggg agctacgaag cccccacgca aggatcagcg tcttccctcg tgaacggggt     8640

tgttagactc ctgtcaaagc cttgggacgt ggtgactgga gttacaggaa tagccatgac     8700

tgacaccaca ccatacggcc aacaaagagt cttcaaagaa aaagtggaca ccagggtgcc     8760

agatccccaa gaaggcactc gccaggtaat gaacatagtc tcttcctggc tgtggaagga     8820

gctggggaaa cgcaagcggc cacgcgtctg caccaaagaa gagtttatca acaaggtgcg     8880

cagcaatgca gcactgggag caatatttga agaggaaaaa gaatggaaga cggctgtgga     8940

agctgtgaat gatccaaggt tttgggccct agtggatagg gagagagaac accacctgag     9000

aggagagtgt cacagctgtg tgtacaacat gatgggaaaa agagaaaaga agcaaggaga     9060

gttcgggaaa gcaaaaggta gccgcgccat ctggtacatg tggttgggag ccagattctt     9120

ggagtttgaa gcccttggat tcttgaacga ggaccattgg atgggaagag aaaactcagg     9180

aggtggagtc gaagggttag gattgcaaag acttggatac attctagaag aaatgaatcg     9240

ggcaccagga ggaaagatgt acgcagatga cactgctggc tgggacaccc gcattagtaa     9300

gtttgatctg gagaatgaag ctctgattac caaccaaatg gaggaagggc acagaactct     9360

ggcgttggcc gtgattaaat acacatacca aaacaaagtg gtgaaggttc tcagaccagc     9420

tgaaggagga aaaacagtta tggacatcat ttcaagacaa gaccagagag ggagtggaca     9480

agttgtcact tatgctctca acacattcac caacttggtg gtgcagctta tccggaacat     9540

ggaagctgag gaagtgttag agatgcaaga cttatggttg ttgaggaagc cagagaaagt     9600

gaccagatgg ttgcagagca atggatggga tagactcaaa cgaatggcgg tcagtggaga     9660

tgactgcgtt gtgaagccaa tcgatgatag gtttgcacat gccctcaggt tcttgaatga     9720

catgggaaaa gttaggaaag acacacagga gtggaaaccc tcgactggat ggagcaattg     9780

ggaagaagtc ccgttctgct cccaccactt caacaagctg tacctcaagg atgggagatc     9840

cattgtggtc ccttgccgcc accaagatga actgattggc cgagctcgcg tctcaccagg     9900

ggcaggatgg agcatccggg agactgcctg tcttgcaaaa tcatatgcgc agatgtggca     9960

gctcctttat ttccacagaa gagaccttcg actgatggct aatgccattt gctcggctgt    10020

gccagttgac tgggtaccaa ctgggagaac cacctggtca atccatggaa agggagaatg    10080

gatgaccact gaggacatgc tcatggtgtg gaatagagtg tggattgagg agaacgacca    10140

tatggaggac aagactcctg taacaaaatg gacagacatt ccctatctag gaaaaaggga    10200

ggacttatgg tgtggatccc ttatagggca cagaccccgc accacttggg ctgaaaacat    10260

caaagacaca gtcaacatgg tgcgcaggat cataggtgat gaagaaaagt acatggacta    10320

tctatccacc caagtccgct acttgggtga ggaagggtcc acacccggag tgttgtaagc    10380

accaatttta gtgttgtcag gcctgctagt cagccacagt ttggggaaag ctgtgcagcc    10440

tgtaaccccc ccaggagaag ctgggaaacc aagctcatag tcaggccgag aacgccatgg    10500

cacggaagaa gccatgctgc ctgtgagccc ctcagaggac actgagtcaa aaaaccccac    10560

gcgcttggaa gcgcaggatg ggaaaagaag gtggcgacct tccccaccct tcaatctggg    10620

gcctgaactg gagactagct gtgaatctcc agcagaggga ctagtggtta gaggagaccc    10680

cccggaaaac gcaaaacagc atattgacgc tgggaaagac cagagactcc atgagtttcc    10740

accacgctgg ccgccaggca cagatcgccg aacagcggcg gccggtgtgg ggaaatccat    10800

ggtttct                                                              10807


<210>  7
<211>  1512
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequences of the E gene from chimeric ZIKALIVax virus (1512 nt; 
       504 aa)

<400>  7
atcaggtgca taggagtcag caatagggac tttgtggaag gtatgtcagg tgggacttgg       60

gttgatgttg tcttggaaca tggaggttgt gtcaccgtaa tggcacagga caaaccgact      120

gtcgacatag agctggttac aacaacagtc agcaacatgg cggaggtacg atcgtactgc      180

tatgaggcat caatatcaga catggcttcg gacagccgct gcccaacaca aggtgaagcc      240

taccttgaca agcaatcaga cactcaatat gtctgcaaaa gaacgttagt ggacagaggc      300

tggggaaatg gatgtggact ttttggcaaa gggagcctgg tgacatgcgc taagtttgca      360

tgctccaaga aaatgaccgg gaagagcatc cagccagaga atctggagta ccggataatg      420

ctgtcagttc atggctccca gcacagtggg atgactgtca atgatatagg atatgaaact      480

gatgagaata gagcgaaagt tgagataacg cccaattcac caagagccga agccaccctg      540

gggggttttg gaagcctagg acttgattgt gaaccgagga caggccttga cttttcagat      600

ttgtattact tgactatgaa taacaagcac tggttggttc acaaggagtg gttccacgac      660

attccattac cttggcacgc tggggcagac accggaactc cacactggaa caacaaagaa      720

gcactggtag agttcaagga cgcacatgcc aaaaggcaaa ctgtcgtggt tctagggagt      780

caagaaggag cagttcacac ggcccttgct ggagctctgg aggctgagat ggatggtgca      840

aagggaaggc tgtcctctgg ccacttgaaa tgtcgcctga aaatggataa acttagattg      900

aagggcgtgt catactcctt gtgtactgca gcgttcacat tcaccaagat cccggctgaa      960

acactgcacg ggacagtcac agtggaggta cagtacgcag ggacagatgg accttgcaag     1020

gttccagctc agatggcggt ggacatgcaa actctgaccc cagttgggag gttgataacc     1080

gctaaccccg taatcactga aagcactgag aactctaaga tgatgctgga acttgatcca     1140

ccatttgggg actcttacat tgtcatagga gtcggggaga agaagatcac ccaccactgg     1200

cacaggagtg gcagcaccat tggaaaagca tttgaggcca ctgtgagagg cgccaagaga     1260

atggcagtcc tgggggatac agcctgggac ttcggatcag tcgggggtgt gttcaactca     1320

ctgggtaagg gcattcacca gatttttgga gcagccttca aatcactgtt tggaggaatg     1380

tcctggttct cacagatcct cataggcacg ctgctagtgt ggttaggttt gaacacaaag     1440

aatggatcta tctccctcac atgcttggcc ctggggggag tgatgatctt cctctccacg     1500

gctgtttctg ct                                                         1512


