                         SEQUENCE LISTING

<110>  PharmaZell GmbH
 
<120>  Coupled, self-sufficient biotransformation of chenodeoxycholic 
       acid  to ursodeoxy-cholic acid and novel enzyme mutants 
       applicable in said process

<130>  M/56325-PCT

<160>  56    

<170>  PatentIn version 3.5

<210>  1
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  1
ccgccgcata tggaaaaatt acaaggaaaa att                                    33


<210>  2
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  2
cgcctagcgg ccgcttatcc taattatcct aatatat                                37


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  3
aaccaaccat gggaatgaaa gttattgtag ta                                     32


<210>  4
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  4
ataataactc gagcgtatag tttaagac                                          28


<210>  5
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  5
aaattgcagt agttactgca gcaacagcag gtattggatt agcatcag                    48


<210>  6
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  6
ctgatgctaa tccaatacct gctgttgctg cagtaactac tgcaattt                    48


<210>  7
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  7
aaattgcagt agttactgca gcaacaggag gtattggatt agcatcag                    48


<210>  8
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  8
ctgatgctaa tccaatacct cctgttgctg cagtaactac tgcaattt                    48


<210>  9
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  9
ttgcagtagt tactgcagca acagatggta ttggattagc atcag                       45


<210>  10
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  10
ctgatgctaa tccaatacca tctgttgctg cagtaactac tgcaa                       45


<210>  11
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  11
gagcaactgt gtacttagca gagcgttcag aagaattagc tcat                        44


<210>  12
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  12
atgagctaat tcttctgaac gctctgctaa gtacacagtt gctc                        44


<210>  13
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  13
gcaactgtgt acttagcaga tcgttcagaa gaattagct                              39


<210>  14
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  14
agctaattct tctgaacgat ctgctaagta cacagttgc                              39


<210>  15
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  15
aaatggagca actgtgtact tagcagatat ttcagaagaa ttagctcatg aagt             54


<210>  16
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  16
taacttcatg agctaattct tctgaaatat ctgctaagta cacagttgct ccattt           56


<210>  17
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  17
gagcaactgt gtacttagca gatctttcag aagaattagc tcatga                      46


<210>  18
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  18
tcatgagcta attcttctga aagatctgct aagtacacag ttgctc                      46


<210>  19
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  19
catctgtggt ggtcagtggt aggaacgccg acgcagctaa c                           41


<210>  20
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  20
gttagctgcg tcggcgttcc taccactgac caccacagat g                           41


<210>  21
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  21
catctgtggt ggtcagtgct aggaacgccg acgcagctaa c                           41


<210>  22
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  22
gttagctgcg tcggcgttcc tagcactgac caccacagat g                           41


<210>  23
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  23
gtggtggtca gtggtattaa cgccgac                                           27


<210>  24
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  24
gtcggcgtta ataccaactg accaccac                                          28


<210>  25
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  25
gcatctgtgg tggtcagtga taggaacgcc gacgca                                 36


<210>  26
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  26
tgcgtcggcg ttcctatcac tgaccaccac agatgc                                 36


<210>  27
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  27
gtcgtcatgg tcagccgtcg cgaggag                                           27


<210>  28
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  28
ctcctcgcga cggctgacca tgacgac                                           27


<210>  29
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  29
gtcgtcatgg tcgagcgtcg cgaggag                                           27


<210>  30
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  30
ctcctcgcga cgctcgacca tgacgac                                           27


<210>  31
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  31
accaaggtcg tggaggccga ctttagc                                           27


<210>  32
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  32
gctaaagtcg gcctccacga ccttggt                                           27


<210>  33
<211>  789
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  CDS
<222>  (1)..(789)

<400>  33
atg gaa aaa tta caa gga aaa att gca gta gtt act gca gca aca aaa         48
Met Glu Lys Leu Gln Gly Lys Ile Ala Val Val Thr Ala Ala Thr Lys           
1               5                   10                  15                

ggt att gga tta gca tca gca gag ata tta gca aaa aat gga gca act         96
Gly Ile Gly Leu Ala Ser Ala Glu Ile Leu Ala Lys Asn Gly Ala Thr           
            20                  25                  30                    

gtg tac tta gca gct cgt tca gaa gaa tta gct cat gaa gtt ata aat        144
Val Tyr Leu Ala Ala Arg Ser Glu Glu Leu Ala His Glu Val Ile Asn           
        35                  40                  45                        

aaa ata agt gca gaa ggt ggt tgt gct aag ttt gtt tac ttt aat gct        192
Lys Ile Ser Ala Glu Gly Gly Cys Ala Lys Phe Val Tyr Phe Asn Ala           
    50                  55                  60                            

cgt gaa gaa gag acc ttt acc tca atg ata gaa gag gta gtt aaa aaa        240
Arg Glu Glu Glu Thr Phe Thr Ser Met Ile Glu Glu Val Val Lys Lys           
65                  70                  75                  80            

gaa ggt aag ata gat ata ctt gta aac aat ttt ggt tca aca aac cct        288
Glu Gly Lys Ile Asp Ile Leu Val Asn Asn Phe Gly Ser Thr Asn Pro           
                85                  90                  95                

tct ctt gac aaa gac ctt gta act ggg gat aca gat aat ttc ttt gac        336
Ser Leu Asp Lys Asp Leu Val Thr Gly Asp Thr Asp Asn Phe Phe Asp           
            100                 105                 110                   

aca gta aat act aat tta aaa agt gtg tat tta cca tgt aaa gca gca        384
Thr Val Asn Thr Asn Leu Lys Ser Val Tyr Leu Pro Cys Lys Ala Ala           
        115                 120                 125                       

att cct cat atg ata aag aat gga aaa gga agt ata gta aat ata tca        432
Ile Pro His Met Ile Lys Asn Gly Lys Gly Ser Ile Val Asn Ile Ser           
    130                 135                 140                           

agt ata ggt tca gta tta cct gat tta tct aga ata gct tac tgt gta        480
Ser Ile Gly Ser Val Leu Pro Asp Leu Ser Arg Ile Ala Tyr Cys Val           
145                 150                 155                 160           

tca aaa gca gca atc aac tca tta act caa aac ata gct aca caa tat        528
Ser Lys Ala Ala Ile Asn Ser Leu Thr Gln Asn Ile Ala Thr Gln Tyr           
                165                 170                 175               

gct aaa gac aat gtt aga tgt aat gct gta ctt cca ggg ctt att gca        576
Ala Lys Asp Asn Val Arg Cys Asn Ala Val Leu Pro Gly Leu Ile Ala           
            180                 185                 190                   

act aaa gca gca tta gac aat atg tct cca gag ttc ata aaa gaa ttt        624
Thr Lys Ala Ala Leu Asp Asn Met Ser Pro Glu Phe Ile Lys Glu Phe           
        195                 200                 205                       

tta aag cat gtt cct tta aat cgt ata ggg gaa cca gat gat ata gca        672
Leu Lys His Val Pro Leu Asn Arg Ile Gly Glu Pro Asp Asp Ile Ala           
    210                 215                 220                           

aaa gct gtt cta ttc tat gct agt gat gac tca tca ttt ata aca ggg        720
Lys Ala Val Leu Phe Tyr Ala Ser Asp Asp Ser Ser Phe Ile Thr Gly           
225                 230                 235                 240           

gat tta ctt gaa gtt gca gga gga ttt ggt tta cct act cca caa ttt        768
Asp Leu Leu Glu Val Ala Gly Gly Phe Gly Leu Pro Thr Pro Gln Phe           
                245                 250                 255               

gca gat aat ata tta gga taa                                            789
Ala Asp Asn Ile Leu Gly                                                   
            260                                                           


<210>  34
<211>  262
<212>  PRT
<213>  Clostridium difficile

<400>  34

Met Glu Lys Leu Gln Gly Lys Ile Ala Val Val Thr Ala Ala Thr Lys 
1               5                   10                  15      


Gly Ile Gly Leu Ala Ser Ala Glu Ile Leu Ala Lys Asn Gly Ala Thr 
            20                  25                  30          


Val Tyr Leu Ala Ala Arg Ser Glu Glu Leu Ala His Glu Val Ile Asn 
        35                  40                  45              


Lys Ile Ser Ala Glu Gly Gly Cys Ala Lys Phe Val Tyr Phe Asn Ala 
    50                  55                  60                  


Arg Glu Glu Glu Thr Phe Thr Ser Met Ile Glu Glu Val Val Lys Lys 
65                  70                  75                  80  


Glu Gly Lys Ile Asp Ile Leu Val Asn Asn Phe Gly Ser Thr Asn Pro 
                85                  90                  95      


Ser Leu Asp Lys Asp Leu Val Thr Gly Asp Thr Asp Asn Phe Phe Asp 
            100                 105                 110         


Thr Val Asn Thr Asn Leu Lys Ser Val Tyr Leu Pro Cys Lys Ala Ala 
        115                 120                 125             


Ile Pro His Met Ile Lys Asn Gly Lys Gly Ser Ile Val Asn Ile Ser 
    130                 135                 140                 


Ser Ile Gly Ser Val Leu Pro Asp Leu Ser Arg Ile Ala Tyr Cys Val 
145                 150                 155                 160 


Ser Lys Ala Ala Ile Asn Ser Leu Thr Gln Asn Ile Ala Thr Gln Tyr 
                165                 170                 175     


Ala Lys Asp Asn Val Arg Cys Asn Ala Val Leu Pro Gly Leu Ile Ala 
            180                 185                 190         


Thr Lys Ala Ala Leu Asp Asn Met Ser Pro Glu Phe Ile Lys Glu Phe 
        195                 200                 205             


Leu Lys His Val Pro Leu Asn Arg Ile Gly Glu Pro Asp Asp Ile Ala 
    210                 215                 220                 


Lys Ala Val Leu Phe Tyr Ala Ser Asp Asp Ser Ser Phe Ile Thr Gly 
225                 230                 235                 240 


Asp Leu Leu Glu Val Ala Gly Gly Phe Gly Leu Pro Thr Pro Gln Phe 
                245                 250                 255     


Ala Asp Asn Ile Leu Gly 
            260         


<210>  35
<211>  282
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7alpha HSDH C. difficile + N-terminal HisTag

<400>  35

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Glu Lys Leu Gln Gly Lys Ile Ala Val Val Thr 
            20                  25                  30          


Ala Ala Thr Lys Gly Ile Gly Leu Ala Ser Ala Glu Ile Leu Ala Lys 
        35                  40                  45              


Asn Gly Ala Thr Val Tyr Leu Ala Ala Arg Ser Glu Glu Leu Ala His 
    50                  55                  60                  


Glu Val Ile Asn Lys Ile Ser Ala Glu Gly Gly Cys Ala Lys Phe Val 
65                  70                  75                  80  


Tyr Phe Asn Ala Arg Glu Glu Glu Thr Phe Thr Ser Met Ile Glu Glu 
                85                  90                  95      


Val Val Lys Lys Glu Gly Lys Ile Asp Ile Leu Val Asn Asn Phe Gly 
            100                 105                 110         


Ser Thr Asn Pro Ser Leu Asp Lys Asp Leu Val Thr Gly Asp Thr Asp 
        115                 120                 125             


Asn Phe Phe Asp Thr Val Asn Thr Asn Leu Lys Ser Val Tyr Leu Pro 
    130                 135                 140                 


Cys Lys Ala Ala Ile Pro His Met Ile Lys Asn Gly Lys Gly Ser Ile 
145                 150                 155                 160 


Val Asn Ile Ser Ser Ile Gly Ser Val Leu Pro Asp Leu Ser Arg Ile 
                165                 170                 175     


Ala Tyr Cys Val Ser Lys Ala Ala Ile Asn Ser Leu Thr Gln Asn Ile 
            180                 185                 190         


Ala Thr Gln Tyr Ala Lys Asp Asn Val Arg Cys Asn Ala Val Leu Pro 
        195                 200                 205             


Gly Leu Ile Ala Thr Lys Ala Ala Leu Asp Asn Met Ser Pro Glu Phe 
    210                 215                 220                 


Ile Lys Glu Phe Leu Lys His Val Pro Leu Asn Arg Ile Gly Glu Pro 
225                 230                 235                 240 


Asp Asp Ile Ala Lys Ala Val Leu Phe Tyr Ala Ser Asp Asp Ser Ser 
                245                 250                 255     


Phe Ile Thr Gly Asp Leu Leu Glu Val Ala Gly Gly Phe Gly Leu Pro 
            260                 265                 270         


Thr Pro Gln Phe Ala Asp Asn Ile Leu Gly 
        275                 280         


<210>  36
<211>  768
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(768)

<400>  36
atg ttt aat tct gac aac ctg aga ctc gac gga aaa tgc gcc atc atc         48
Met Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile           
1               5                   10                  15                

aca ggt gcg ggt gca ggt att ggt aaa gaa atc gcc att aca ttc gcg         96
Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala           
            20                  25                  30                    

aca gct ggc gca tct gtg gtg gtc agt gat att aac gcc gac gca gct        144
Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala           
        35                  40                  45                        

aac cat gtt gta gac gaa att caa caa ctg ggt ggt cag gca ttt gcc        192
Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala           
    50                  55                  60                            

tgc cgt tgt gat att act tcc gaa cag gaa ctc tct gca ctg gca gac        240
Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp           
65                  70                  75                  80            

ttt gct atc agt aag ctg ggt aaa gtt gat att ctg gtt aac aac gcc        288
Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala           
                85                  90                  95                

ggt ggc ggt gga cct aaa ccg ttt gat atg cca atg gcg gat ttt cgc        336
Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg           
            100                 105                 110                   

cgt gct tat gaa ctg aat gtg ttt tct ttt ttc cat ctg tca caa ctt        384
Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu           
        115                 120                 125                       

gtt gcg cca gaa atg gaa aaa aat ggc ggt ggc gtt att ctg acc atc        432
Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile           
    130                 135                 140                           

act tct atg gcg gca gaa aat aaa aat ata aac atg act tcc tat gca        480
Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala           
145                 150                 155                 160           

tca tct aaa gct gcg gcc agt cat ctg gtc aga aat atg gcg ttt gac        528
Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp           
                165                 170                 175               

ctg ggt gaa aaa aat att cgg gta aat ggc att gcg ccg ggg gca ata        576
Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile           
            180                 185                 190                   

tta acc gat gcc ctg aaa tcc gtt att aca cca gaa att gaa caa aaa        624
Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys           
        195                 200                 205                       

atg tta cag cac acg ccg atc aga cgt ctg ggc caa ccg caa gat att        672
Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile           
    210                 215                 220                           

gct aac gca gcg ctg ttc ctt tgc tcg cct gct gcg agc tgg gta agc        720
Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser           
225                 230                 235                 240           

gga caa att ctc acc gtc tcc ggt ggt ggg gta cag gag ctc aat taa        768
Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn               
                245                 250                 255               


<210>  37
<211>  255
<212>  PRT
<213>  Escherichia coli

<400>  37

Met Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile 
1               5                   10                  15      


Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala 
            20                  25                  30          


Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala 
        35                  40                  45              


Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 
    50                  55                  60                  


Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 
65                  70                  75                  80  


Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala 
                85                  90                  95      


Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg 
            100                 105                 110         


Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu 
        115                 120                 125             


Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile 
    130                 135                 140                 


Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala 
145                 150                 155                 160 


Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp 
                165                 170                 175     


Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 
            180                 185                 190         


Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 
        195                 200                 205             


Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile 
    210                 215                 220                 


Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser 
225                 230                 235                 240 


Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn 
                245                 250                 255 


<210>  38
<211>  275
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. coli  7alpha  HSDH WT + N-terminal His Tag

<400>  38

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys 
            20                  25                  30          


Cys Ala Ile Ile Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala 
        35                  40                  45              


Ile Thr Phe Ala Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn 
    50                  55                  60                  


Ala Asp Ala Ala Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly 
65                  70                  75                  80  


Gln Ala Phe Ala Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser 
                85                  90                  95      


Ala Leu Ala Asp Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu 
            100                 105                 110         


Val Asn Asn Ala Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met 
        115                 120                 125             


Ala Asp Phe Arg Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His 
    130                 135                 140                 


Leu Ser Gln Leu Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val 
145                 150                 155                 160 


Ile Leu Thr Ile Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met 
                165                 170                 175     


Thr Ser Tyr Ala Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn 
            180                 185                 190         


Met Ala Phe Asp Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala 
        195                 200                 205             


Pro Gly Ala Ile Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu 
    210                 215                 220                 


Ile Glu Gln Lys Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln 
225                 230                 235                 240 


Pro Gln Asp Ile Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala 
                245                 250                 255     


Ser Trp Val Ser Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln 
            260                 265                 270         


Glu Leu Asn 
        275 


<210>  39
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  7alpha HSDH E. coli mutant D42G/I43R


<220>
<221>  CDS
<222>  (1)..(768)

<400>  39
atg ttt aat tct gac aac ctg aga ctc gac gga aaa tgc gcc atc atc         48
Met Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile           
1               5                   10                  15                

aca ggt gcg ggt gca ggt att ggt aaa gaa atc gcc att aca ttc gcg         96
Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala           
            20                  25                  30                    

aca gct ggc gca tct gtg gtg gtc agt gat att aac gcc gac gca gct        144
Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala           
        35                  40                  45                        

aac cat gtt gta gac gaa att caa caa ctg ggt ggt cag gca ttt gcc        192
Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala           
    50                  55                  60                            

tgc cgt tgt gat att act tcc gaa cag gaa ctc tct gca ctg gca gac        240
Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp           
65                  70                  75                  80            

ttt gct atc agt aag ctg ggt aaa gtt gat att ctg gtt aac aac gcc        288
Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala           
                85                  90                  95                

ggt ggc ggt gga cct aaa ccg ttt gat atg cca atg gcg gat ttt cgc        336
Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg           
            100                 105                 110                   

cgt gct tat gaa ctg aat gtg ttt tct ttt ttc cat ctg tca caa ctt        384
Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu           
        115                 120                 125                       

gtt gcg cca gaa atg gaa aaa aat ggc ggt ggc gtt att ctg acc atc        432
Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile           
    130                 135                 140                           

act tct atg gcg gca gaa aat aaa aat ata aac atg act tcc tat gca        480
Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala           
145                 150                 155                 160           

tca tct aaa gct gcg gcc agt cat ctg gtc aga aat atg gcg ttt gac        528
Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp           
                165                 170                 175               

ctg ggt gaa aaa aat att cgg gta aat ggc att gcg ccg ggg gca ata        576
Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile           
            180                 185                 190                   

tta acc gat gcc ctg aaa tcc gtt att aca cca gaa att gaa caa aaa        624
Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys           
        195                 200                 205                       

atg tta cag cac acg ccg atc aga cgt ctg ggc caa ccg caa gat att        672
Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile           
    210                 215                 220                           

gct aac gca gcg ctg ttc ctt tgc tcg cct gct gcg agc tgg gta agc        720
Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser           
225                 230                 235                 240           

gga caa att ctc acc gtc tcc ggt ggt ggg gta cag gag ctc aat taa        768
Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn               
                245                 250                 255               


<210>  40
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  40

Met Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile 
1               5                   10                  15      


Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala 
            20                  25                  30          


Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala 
        35                  40                  45              


Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 
    50                  55                  60                  


Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 
65                  70                  75                  80  


Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala 
                85                  90                  95      


Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg 
            100                 105                 110         


Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu 
        115                 120                 125             


Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile 
    130                 135                 140                 


Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala 
145                 150                 155                 160 


Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp 
                165                 170                 175     


Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 
            180                 185                 190         


Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 
        195                 200                 205             


Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile 
    210                 215                 220                 


Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser 
225                 230                 235                 240 


Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn 
                245                 250                 255 


<210>  41
<211>  275
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7alpha HSDH E. coli mutant D42G/I43R + N-terminal His-Tag

<400>  41

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys 
            20                  25                  30          


Cys Ala Ile Ile Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala 
        35                  40                  45              


Ile Thr Phe Ala Thr Ala Gly Ala Ser Val Val Val Ser Gly Arg Asn 
    50                  55                  60                  


Ala Asp Ala Ala Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly 
65                  70                  75                  80  


Gln Ala Phe Ala Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser 
                85                  90                  95      


Ala Leu Ala Asp Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu 
            100                 105                 110         


Val Asn Asn Ala Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met 
        115                 120                 125             


Ala Asp Phe Arg Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His 
    130                 135                 140                 


Leu Ser Gln Leu Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val 
145                 150                 155                 160 


Ile Leu Thr Ile Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met 
                165                 170                 175     


Thr Ser Tyr Ala Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn 
            180                 185                 190         


Met Ala Phe Asp Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala 
        195                 200                 205             


Pro Gly Ala Ile Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu 
    210                 215                 220                 


Ile Glu Gln Lys Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln 
225                 230                 235                 240 


Pro Gln Asp Ile Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala 
                245                 250                 255     


Ser Trp Val Ser Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln 
            260                 265                 270         


Glu Leu Asn 
        275 


<210>  42
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7alpha HSDH E. coli mutant D42G/I43R +  C-terminal His-Tag

<400>  42

Met Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile 
1               5                   10                  15      


Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala 
            20                  25                  30          


Thr Ala Gly Ala Ser Val Val Val Ser Gly Arg Asn Ala Asp Ala Ala 
        35                  40                  45              


Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 
    50                  55                  60                  


Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 
65                  70                  75                  80  


Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala 
                85                  90                  95      


Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg 
            100                 105                 110         


Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu 
        115                 120                 125             


Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile 
    130                 135                 140                 


Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala 
145                 150                 155                 160 


Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp 
                165                 170                 175     


Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 
            180                 185                 190         


Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 
        195                 200                 205             


Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile 
    210                 215                 220                 


Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser 
225                 230                 235                 240 


Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn Leu 
                245                 250                 255     


Glu His His His His His His 
            260             


<210>  43
<211>  792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  7-HSDH Mutant [G39S/R64E] C. aerofaciensi


<220>
<221>  CDS
<222>  (1)..(792)

<400>  43
atg aac ctg agg gag aag tac ggt gag tgg ggc ctg atc ctg ggc gcg         48
Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala           
1               5                   10                  15                

acc gag ggc gtc ggc aag gcg ttc tgc gag aag atc gcc gcc ggc ggc         96
Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly           
            20                  25                  30                    

atg aac gtc gtc atg gtc agc cgt cgc gag gag aag ctg aac gtg ctc        144
Met Asn Val Val Met Val Ser Arg Arg Glu Glu Lys Leu Asn Val Leu           
        35                  40                  45                        

gca ggc gag atc cgc gag acc tac ggc gtg gag acc aag gtc gtg gag        192
Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Glu           
    50                  55                  60                            

gcc gac ttt agc cag ccc ggc gct gcc gag acc gtc ttc gcc gcg acc        240
Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr           
65                  70                  75                  80            

gag ggc ctg gac atg ggc ttc atg agc tac gtg gcc tgc ctg cac agc        288
Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser           
                85                  90                  95                

ttc ggt aag atc cag gac acc ccc tgg gag aag cac gag gcc atg atc        336
Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile           
            100                 105                 110                   

aac gtc aac gtc gtg acc ttc ctc aag tgc ttc cac cac tac atg cgg        384
Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg           
        115                 120                 125                       

atc ttt gcc gcc cag gac cgc ggc gcc gtg atc aac gtc tcg tcg atg        432
Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met           
    130                 135                 140                           

acc ggc atc agc tcc agc ccc tgg aac ggc cag tac ggc gcg ggc aag        480
Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys           
145                 150                 155                 160           

gcc ttc atc ctc aag atg acc gag gcc gtg gcc tgc gag tgc gag ggc        528
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly           
                165                 170                 175               

acc ggc gtc gac gtc gag gtc atc acc ctc ggc acc acc cta acc ccc        576
Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro           
            180                 185                 190                   

agc ctg ctg tcc aac ctc ccc ggc ggc ccg cag ggc gag gcc gtc atg        624
Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met           
        195                 200                 205                       

aag atc gcc ctc acc ccc gag gag tgc gtt gac gag gcc ttt gag aag        672
Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys           
    210                 215                 220                           

ctg ggt aag gag ctc tcc gtc atc gcc ggc cag cgc aac aag gac tcc        720
Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser           
225                 230                 235                 240           

gtc cac gac tgg aag gca aac cac acc gag gac gag tac atc cgc tac        768
Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr           
                245                 250                 255               

atg ggg tcg ttc tac cgc gac tag                                        792
Met Gly Ser Phe Tyr Arg Asp                                               
            260                                                           


<210>  44
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  44

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Ser Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Glu 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  45
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7-HSDH Mutant [G39S/R64E]  C. aerofaciens  + C-terminal His-tag

<400>  45

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Ser Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Glu 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp Leu Glu His His His His His His 
            260                 265                 270     


<210>  46
<211>  792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  7-HSDH Mutante [G39E]   C. aerofaciens


<220>
<221>  CDS
<222>  (1)..(792)

<400>  46
atg aac ctg agg gag aag tac ggt gag tgg ggc ctg atc ctg ggc gcg         48
Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala           
1               5                   10                  15                

acc gag ggc gtc ggc aag gcg ttc tgc gag aag atc gcc gcc ggc ggc         96
Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly           
            20                  25                  30                    

atg aac gtc gtc atg gtc gag cgt cgc gag gag aag ctg aac gtg ctc        144
Met Asn Val Val Met Val Glu Arg Arg Glu Glu Lys Leu Asn Val Leu           
        35                  40                  45                        

gca ggc gag atc cgc gag acc tac ggc gtg gag acc aag gtc gtg cgc        192
Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg           
    50                  55                  60                            

gcc gac ttt agc cag ccc ggc gct gcc gag acc gtc ttc gcc gcg acc        240
Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr           
65                  70                  75                  80            

gag ggc ctg gac atg ggc ttc atg agc tac gtg gcc tgc ctg cac agc        288
Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser           
                85                  90                  95                

ttc ggt aag atc cag gac acc ccc tgg gag aag cac gag gcc atg atc        336
Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile           
            100                 105                 110                   

aac gtc aac gtc gtg acc ttc ctc aag tgc ttc cac cac tac atg cgg        384
Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg           
        115                 120                 125                       

atc ttt gcc gcc cag gac cgc ggc gcc gtg atc aac gtc tcg tcg atg        432
Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met           
    130                 135                 140                           

acc ggc atc agc tcc agc ccc tgg aac ggc cag tac ggc gcg ggc aag        480
Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys           
145                 150                 155                 160           

gcc ttc atc ctc aag atg acc gag gcc gtg gcc tgc gag tgc gag ggc        528
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly           
                165                 170                 175               

acc ggc gtc gac gtc gag gtc atc acc ctc ggc acc acc cta acc ccc        576
Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro           
            180                 185                 190                   

agc ctg ctg tcc aac ctc ccc ggc ggc ccg cag ggc gag gcc gtc atg        624
Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met           
        195                 200                 205                       

aag atc gcc ctc acc ccc gag gag tgc gtt gac gag gcc ttt gag aag        672
Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys           
    210                 215                 220                           

ctg ggt aag gag ctc tcc gtc atc gcc ggc cag cgc aac aag gac tcc        720
Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser           
225                 230                 235                 240           

gtc cac gac tgg aag gca aac cac acc gag gac gag tac atc cgc tac        768
Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr           
                245                 250                 255               

atg ggg tcg ttc tac cgc gac tag                                        792
Met Gly Ser Phe Tyr Arg Asp                                               
            260                                                           


<210>  47
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  47

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Glu Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  48
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7-HSDH Mutante [G39E]  C. aerofaciens + C-terminal His-Tag

<400>  48

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Glu Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp Leu Glu His His His His His His 
            260                 265                 270     


<210>  49
<211>  1359
<212>  DNA
<213>  Lactobacillus sanfrancisco


<220>
<221>  CDS
<222>  (1)..(1359)

<400>  49
atg aaa gtt att gta gta ggt tgt act cac gct ggc act ttt gca gtt         48
Met Lys Val Ile Val Val Gly Cys Thr His Ala Gly Thr Phe Ala Val           
1               5                   10                  15                

aag caa acg att gcc gat cac ccc gat gca gat gtg act gca tat gaa         96
Lys Gln Thr Ile Ala Asp His Pro Asp Ala Asp Val Thr Ala Tyr Glu           
            20                  25                  30                    

atg aat gat aac att tcc ttt tta tca tgt gga atc gcc ctt tac tta        144
Met Asn Asp Asn Ile Ser Phe Leu Ser Cys Gly Ile Ala Leu Tyr Leu           
        35                  40                  45                        

ggt aaa gaa att aaa aac aat gat ccc cga ggg ctt ttc tac tca agt        192
Gly Lys Glu Ile Lys Asn Asn Asp Pro Arg Gly Leu Phe Tyr Ser Ser           
    50                  55                  60                            

cca gaa gaa tta agc aat ctt gga gct aac gtc caa atg cgt cat caa        240
Pro Glu Glu Leu Ser Asn Leu Gly Ala Asn Val Gln Met Arg His Gln           
65                  70                  75                  80            

gtt aca aac gtt gat cca gaa aca aaa aca atc aaa gtt aaa gat tta        288
Val Thr Asn Val Asp Pro Glu Thr Lys Thr Ile Lys Val Lys Asp Leu           
                85                  90                  95                

atc acc aac gaa gaa aaa aca gaa gca tat gac aaa tta att atg acc        336
Ile Thr Asn Glu Glu Lys Thr Glu Ala Tyr Asp Lys Leu Ile Met Thr           
            100                 105                 110                   

act ggt tct aag cct act gtt cct cca atc cct gga atc gat agt agt        384
Thr Gly Ser Lys Pro Thr Val Pro Pro Ile Pro Gly Ile Asp Ser Ser           
        115                 120                 125                       

cgc gtt tac ctt tgt aaa aac tat aac gat gct aaa aag tta ttt gaa        432
Arg Val Tyr Leu Cys Lys Asn Tyr Asn Asp Ala Lys Lys Leu Phe Glu           
    130                 135                 140                           

gaa gct ccc aaa gct aaa acg att act atc att ggt tct ggt tat att        480
Glu Ala Pro Lys Ala Lys Thr Ile Thr Ile Ile Gly Ser Gly Tyr Ile           
145                 150                 155                 160           

ggt gcc gaa ctg gct gaa gcc tac tca aac caa aat tat aac gtt aat        528
Gly Ala Glu Leu Ala Glu Ala Tyr Ser Asn Gln Asn Tyr Asn Val Asn           
                165                 170                 175               

tta att gat ggt cat gaa cga gtt ctt tac aag tat ttt gat aaa gaa        576
Leu Ile Asp Gly His Glu Arg Val Leu Tyr Lys Tyr Phe Asp Lys Glu           
            180                 185                 190                   

ttt act gat att tta gcc aaa gat tat gaa gct cat ggt gtt aac ctg        624
Phe Thr Asp Ile Leu Ala Lys Asp Tyr Glu Ala His Gly Val Asn Leu           
        195                 200                 205                       

gtt ctt ggt tca aaa gta gct gct ttt gaa gaa gtc gat gat gaa att        672
Val Leu Gly Ser Lys Val Ala Ala Phe Glu Glu Val Asp Asp Glu Ile           
    210                 215                 220                           

atc act aaa acc cta gat ggt aaa gaa att aaa tct gat att gca att        720
Ile Thr Lys Thr Leu Asp Gly Lys Glu Ile Lys Ser Asp Ile Ala Ile           
225                 230                 235                 240           

ctt tgt atc ggt ttc cgc cct aac act gaa tta ctt aaa ggt aaa gtt        768
Leu Cys Ile Gly Phe Arg Pro Asn Thr Glu Leu Leu Lys Gly Lys Val           
                245                 250                 255               

gcc atg ttg gat aac ggt gca atc att act gat gaa tac atg cat tca        816
Ala Met Leu Asp Asn Gly Ala Ile Ile Thr Asp Glu Tyr Met His Ser           
            260                 265                 270                   

tca aat cgc gac att ttt gct gct ggt gat agt gcc gcc gtt cac tac        864
Ser Asn Arg Asp Ile Phe Ala Ala Gly Asp Ser Ala Ala Val His Tyr           
        275                 280                 285                       

aac ccc act aat tct aac gcc tac att cct tta gct acc aac gcc gta        912
Asn Pro Thr Asn Ser Asn Ala Tyr Ile Pro Leu Ala Thr Asn Ala Val           
    290                 295                 300                           

cgc caa ggg aga tta gtt ggc cta aat ctg act gaa gac aaa gta aaa        960
Arg Gln Gly Arg Leu Val Gly Leu Asn Leu Thr Glu Asp Lys Val Lys           
305                 310                 315                 320           

gac atg gga acc caa tct tca tct ggt ctt aaa cta tac ggt cgg act       1008
Asp Met Gly Thr Gln Ser Ser Ser Gly Leu Lys Leu Tyr Gly Arg Thr           
                325                 330                 335               

tat gtc tca act gga atc aat acg gct ctt gct aaa gcc aat aat tta       1056
Tyr Val Ser Thr Gly Ile Asn Thr Ala Leu Ala Lys Ala Asn Asn Leu           
            340                 345                 350                   

aaa gtt agc gaa gta atc ata gct gat aat tat cgt cca gaa ttt atg       1104
Lys Val Ser Glu Val Ile Ile Ala Asp Asn Tyr Arg Pro Glu Phe Met           
        355                 360                 365                       

tta tca acg gat gaa gtt tta atg tca tta gtg tat gat cct aag act       1152
Leu Ser Thr Asp Glu Val Leu Met Ser Leu Val Tyr Asp Pro Lys Thr           
    370                 375                 380                           

cgt gta att ttg gga ggg gcg ctt tca agt atg cac gat gtt tcg caa       1200
Arg Val Ile Leu Gly Gly Ala Leu Ser Ser Met His Asp Val Ser Gln           
385                 390                 395                 400           

tca gcg aac gtc tta tca gta tgt att caa aat aaa aac acg att gac       1248
Ser Ala Asn Val Leu Ser Val Cys Ile Gln Asn Lys Asn Thr Ile Asp           
                405                 410                 415               

gat tta gca atg gtg gat atg tta ttc caa cca caa ttt gat cgt ccg       1296
Asp Leu Ala Met Val Asp Met Leu Phe Gln Pro Gln Phe Asp Arg Pro           
            420                 425                 430                   

ttt aac tac tta aac att cta ggc caa gct gct caa gca caa gct gac       1344
Phe Asn Tyr Leu Asn Ile Leu Gly Gln Ala Ala Gln Ala Gln Ala Asp           
        435                 440                 445                       

aaa gca cat aaa taa                                                   1359
Lys Ala His Lys                                                           
    450                                                                   


<210>  50
<211>  452
<212>  PRT
<213>  Lactobacillus sanfrancisco

<400>  50

Met Lys Val Ile Val Val Gly Cys Thr His Ala Gly Thr Phe Ala Val 
1               5                   10                  15      


Lys Gln Thr Ile Ala Asp His Pro Asp Ala Asp Val Thr Ala Tyr Glu 
            20                  25                  30          


Met Asn Asp Asn Ile Ser Phe Leu Ser Cys Gly Ile Ala Leu Tyr Leu 
        35                  40                  45              


Gly Lys Glu Ile Lys Asn Asn Asp Pro Arg Gly Leu Phe Tyr Ser Ser 
    50                  55                  60                  


Pro Glu Glu Leu Ser Asn Leu Gly Ala Asn Val Gln Met Arg His Gln 
65                  70                  75                  80  


Val Thr Asn Val Asp Pro Glu Thr Lys Thr Ile Lys Val Lys Asp Leu 
                85                  90                  95      


Ile Thr Asn Glu Glu Lys Thr Glu Ala Tyr Asp Lys Leu Ile Met Thr 
            100                 105                 110         


Thr Gly Ser Lys Pro Thr Val Pro Pro Ile Pro Gly Ile Asp Ser Ser 
        115                 120                 125             


Arg Val Tyr Leu Cys Lys Asn Tyr Asn Asp Ala Lys Lys Leu Phe Glu 
    130                 135                 140                 


Glu Ala Pro Lys Ala Lys Thr Ile Thr Ile Ile Gly Ser Gly Tyr Ile 
145                 150                 155                 160 


Gly Ala Glu Leu Ala Glu Ala Tyr Ser Asn Gln Asn Tyr Asn Val Asn 
                165                 170                 175     


Leu Ile Asp Gly His Glu Arg Val Leu Tyr Lys Tyr Phe Asp Lys Glu 
            180                 185                 190         


Phe Thr Asp Ile Leu Ala Lys Asp Tyr Glu Ala His Gly Val Asn Leu 
        195                 200                 205             


Val Leu Gly Ser Lys Val Ala Ala Phe Glu Glu Val Asp Asp Glu Ile 
    210                 215                 220                 


Ile Thr Lys Thr Leu Asp Gly Lys Glu Ile Lys Ser Asp Ile Ala Ile 
225                 230                 235                 240 


Leu Cys Ile Gly Phe Arg Pro Asn Thr Glu Leu Leu Lys Gly Lys Val 
                245                 250                 255     


Ala Met Leu Asp Asn Gly Ala Ile Ile Thr Asp Glu Tyr Met His Ser 
            260                 265                 270         


Ser Asn Arg Asp Ile Phe Ala Ala Gly Asp Ser Ala Ala Val His Tyr 
        275                 280                 285             


Asn Pro Thr Asn Ser Asn Ala Tyr Ile Pro Leu Ala Thr Asn Ala Val 
    290                 295                 300                 


Arg Gln Gly Arg Leu Val Gly Leu Asn Leu Thr Glu Asp Lys Val Lys 
305                 310                 315                 320 


Asp Met Gly Thr Gln Ser Ser Ser Gly Leu Lys Leu Tyr Gly Arg Thr 
                325                 330                 335     


Tyr Val Ser Thr Gly Ile Asn Thr Ala Leu Ala Lys Ala Asn Asn Leu 
            340                 345                 350         


Lys Val Ser Glu Val Ile Ile Ala Asp Asn Tyr Arg Pro Glu Phe Met 
        355                 360                 365             


Leu Ser Thr Asp Glu Val Leu Met Ser Leu Val Tyr Asp Pro Lys Thr 
    370                 375                 380                 


Arg Val Ile Leu Gly Gly Ala Leu Ser Ser Met His Asp Val Ser Gln 
385                 390                 395                 400 


Ser Ala Asn Val Leu Ser Val Cys Ile Gln Asn Lys Asn Thr Ile Asp 
                405                 410                 415     


Asp Leu Ala Met Val Asp Met Leu Phe Gln Pro Gln Phe Asp Arg Pro 
            420                 425                 430         


Phe Asn Tyr Leu Asn Ile Leu Gly Gln Ala Ala Gln Ala Gln Ala Asp 
        435                 440                 445             


Lys Ala His Lys 
    450         


<210>  51
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NAD(P)H Oxidase + C-terminal His-Tag

<400>  51

Met Lys Val Ile Val Val Gly Cys Thr His Ala Gly Thr Phe Ala Val 
1               5                   10                  15      


Lys Gln Thr Ile Ala Asp His Pro Asp Ala Asp Val Thr Ala Tyr Glu 
            20                  25                  30          


Met Asn Asp Asn Ile Ser Phe Leu Ser Cys Gly Ile Ala Leu Tyr Leu 
        35                  40                  45              


Gly Lys Glu Ile Lys Asn Asn Asp Pro Arg Gly Leu Phe Tyr Ser Ser 
    50                  55                  60                  


Pro Glu Glu Leu Ser Asn Leu Gly Ala Asn Val Gln Met Arg His Gln 
65                  70                  75                  80  


Val Thr Asn Val Asp Pro Glu Thr Lys Thr Ile Lys Val Lys Asp Leu 
                85                  90                  95      


Ile Thr Asn Glu Glu Lys Thr Glu Ala Tyr Asp Lys Leu Ile Met Thr 
            100                 105                 110         


Thr Gly Ser Lys Pro Thr Val Pro Pro Ile Pro Gly Ile Asp Ser Ser 
        115                 120                 125             


Arg Val Tyr Leu Cys Lys Asn Tyr Asn Asp Ala Lys Lys Leu Phe Glu 
    130                 135                 140                 


Glu Ala Pro Lys Ala Lys Thr Ile Thr Ile Ile Gly Ser Gly Tyr Ile 
145                 150                 155                 160 


Gly Ala Glu Leu Ala Glu Ala Tyr Ser Asn Gln Asn Tyr Asn Val Asn 
                165                 170                 175     


Leu Ile Asp Gly His Glu Arg Val Leu Tyr Lys Tyr Phe Asp Lys Glu 
            180                 185                 190         


Phe Thr Asp Ile Leu Ala Lys Asp Tyr Glu Ala His Gly Val Asn Leu 
        195                 200                 205             


Val Leu Gly Ser Lys Val Ala Ala Phe Glu Glu Val Asp Asp Glu Ile 
    210                 215                 220                 


Ile Thr Lys Thr Leu Asp Gly Lys Glu Ile Lys Ser Asp Ile Ala Ile 
225                 230                 235                 240 


Leu Cys Ile Gly Phe Arg Pro Asn Thr Glu Leu Leu Lys Gly Lys Val 
                245                 250                 255     


Ala Met Leu Asp Asn Gly Ala Ile Ile Thr Asp Glu Tyr Met His Ser 
            260                 265                 270         


Ser Asn Arg Asp Ile Phe Ala Ala Gly Asp Ser Ala Ala Val His Tyr 
        275                 280                 285             


Asn Pro Thr Asn Ser Asn Ala Tyr Ile Pro Leu Ala Thr Asn Ala Val 
    290                 295                 300                 


Arg Gln Gly Arg Leu Val Gly Leu Asn Leu Thr Glu Asp Lys Val Lys 
305                 310                 315                 320 


Asp Met Gly Thr Gln Ser Ser Ser Gly Leu Lys Leu Tyr Gly Arg Thr 
                325                 330                 335     


Tyr Val Ser Thr Gly Ile Asn Thr Ala Leu Ala Lys Ala Asn Asn Leu 
            340                 345                 350         


Lys Val Ser Glu Val Ile Ile Ala Asp Asn Tyr Arg Pro Glu Phe Met 
        355                 360                 365             


Leu Ser Thr Asp Glu Val Leu Met Ser Leu Val Tyr Asp Pro Lys Thr 
    370                 375                 380                 


Arg Val Ile Leu Gly Gly Ala Leu Ser Ser Met His Asp Val Ser Gln 
385                 390                 395                 400 


Ser Ala Asn Val Leu Ser Val Cys Ile Gln Asn Lys Asn Thr Ile Asp 
                405                 410                 415     


Asp Leu Ala Met Val Asp Met Leu Phe Gln Pro Gln Phe Asp Arg Pro 
            420                 425                 430         


Phe Asn Tyr Leu Asn Ile Leu Gly Gln Ala Ala Gln Ala Gln Ala Asp 
        435                 440                 445             


Lys Ala His Lys Leu Glu His His His His His His 
    450                 455                 460 


<210>  52
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7beta-HSDH C. aerofaciens + C-terminal His-tag

<400>  52

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp Leu Glu His His His His His His 
            260                 265                 270     


<210>  53
<211>  792
<212>  DNA
<213>  Colinsella aerofaciens


<220>
<221>  CDS
<222>  (1)..(792)

<400>  53
atg aac ctg agg gag aag tac ggt gag tgg ggc ctg atc ctg ggc gcg         48
Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala           
1               5                   10                  15                

acc gag ggc gtc ggc aag gcg ttc tgc gag aag atc gcc gcc ggc ggc         96
Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly           
            20                  25                  30                    

atg aac gtc gtc atg gtc ggc cgt cgc gag gag aag ctg aac gtg ctc        144
Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu           
        35                  40                  45                        

gca ggc gag atc cgc gag acc tac ggc gtg gag acc aag gtc gtg cgc        192
Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg           
    50                  55                  60                            

gcc gac ttt agc cag ccc ggc gct gcc gag acc gtc ttc gcc gcg acc        240
Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr           
65                  70                  75                  80            

gag ggc ctg gac atg ggc ttc atg agc tac gtg gcc tgc ctg cac agc        288
Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser           
                85                  90                  95                

ttc ggt aag atc cag gac acc ccc tgg gag aag cac gag gcc atg atc        336
Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile           
            100                 105                 110                   

aac gtc aac gtc gtg acc ttc ctc aag tgc ttc cac cac tac atg cgg        384
Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg           
        115                 120                 125                       

atc ttt gcc gcc cag gac cgc ggc gcc gtg atc aac gtc tcg tcg atg        432
Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met           
    130                 135                 140                           

acc ggc atc agc tcc agc ccc tgg aac ggc cag tac ggc gcg ggc aag        480
Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys           
145                 150                 155                 160           

gcc ttc atc ctc aag atg acc gag gcc gtg gcc tgc gag tgc gag ggc        528
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly           
                165                 170                 175               

acc ggc gtc gac gtc gag gtc atc acc ctc ggc acc acc cta acc ccc        576
Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro           
            180                 185                 190                   

agc ctg ctg tcc aac ctc ccc ggc ggc ccg cag ggc gag gcc gtc atg        624
Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met           
        195                 200                 205                       

aag atc gcc ctc acc ccc gag gag tgc gtt gac gag gcc ttt gag aag        672
Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys           
    210                 215                 220                           

ctg ggt aag gag ctc tcc gtc atc gcc ggc cag cgc aac aag gac tcc        720
Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser           
225                 230                 235                 240           

gtc cac gac tgg aag gca aac cac acc gag gac gag tac atc cgc tac        768
Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr           
                245                 250                 255               

atg ggg tcg ttc tac cgc gac tag                                        792
Met Gly Ser Phe Tyr Arg Asp                                               
            260                                                           


<210>  54
<211>  263
<212>  PRT
<213>  Colinsella aerofaciens

<400>  54

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  55
<211>  792
<212>  DNA
<213>  Ruminococcus gnavus


<220>
<221>  CDS
<222>  (1)..(792)

<400>  55
atg aca ttg aga gaa aaa tat gga gaa tgg gga att att tta ggc gct         48
Met Thr Leu Arg Glu Lys Tyr Gly Glu Trp Gly Ile Ile Leu Gly Ala           
1               5                   10                  15                

act gaa ggt gtc gga aaa gca ttt tgt gaa agg ctt gcc aaa gaa ggt         96
Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Arg Leu Ala Lys Glu Gly           
            20                  25                  30                    

atg aat gtc gta atg gtc gga cgc cgt gaa gaa aaa tta aaa gag ctc        144
Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Lys Glu Leu           
        35                  40                  45                        

ggt gag gaa cta aaa aac act tat gag att gat tat aaa gtc gta aaa        192
Gly Glu Glu Leu Lys Asn Thr Tyr Glu Ile Asp Tyr Lys Val Val Lys           
    50                  55                  60                            

gca gac ttt tcg ctg cca gat gct act gac aaa att ttt gct gca aca        240
Ala Asp Phe Ser Leu Pro Asp Ala Thr Asp Lys Ile Phe Ala Ala Thr           
65                  70                  75                  80            

gaa aat ctg gat atg gga ttt atg gcc tat gta gcc tgc tta cac tct        288
Glu Asn Leu Asp Met Gly Phe Met Ala Tyr Val Ala Cys Leu His Ser           
                85                  90                  95                

ttt ggc aaa atc cag gat aca cct tgg gaa aag cat gag gca atg atc        336
Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile           
            100                 105                 110                   

aac gta aac gtt gtt aca ttt atg aaa tgc ttc tat cac tat atg aaa        384
Asn Val Asn Val Val Thr Phe Met Lys Cys Phe Tyr His Tyr Met Lys           
        115                 120                 125                       

atc ttt gct gca cag gat cgc ggt gct gtc atc aac gta tct tct atg        432
Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met           
    130                 135                 140                           

act gga att tcc agt tca cca tgg aat ggc caa tat ggt gca gga aag        480
Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys           
145                 150                 155                 160           

gca ttc att tta aaa atg aca gag gct gtt gcc tgt gaa acg gaa aag        528
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Thr Glu Lys           
                165                 170                 175               

acc aat gtt gat gtg gaa gtc atc act ttg gga act act ctg aca cca        576
Thr Asn Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro           
            180                 185                 190                   

agt ctt tta agc aac ctg cct ggc gga cca cag ggg gaa gct gtt atg        624
Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met           
        195                 200                 205                       

aag act gct caa aca ccg gaa gaa gtt gtg gac gaa gct ttt gaa aaa        672
Lys Thr Ala Gln Thr Pro Glu Glu Val Val Asp Glu Ala Phe Glu Lys           
    210                 215                 220                           

tta gga aaa gaa ctg tct gtc att tcc gga gag cgt aat aaa gcc agc        720
Leu Gly Lys Glu Leu Ser Val Ile Ser Gly Glu Arg Asn Lys Ala Ser           
225                 230                 235                 240           

gtc cat gac tgg aaa gcg aat cat aca gaa gat gac tat atc cgc tat        768
Val His Asp Trp Lys Ala Asn His Thr Glu Asp Asp Tyr Ile Arg Tyr           
                245                 250                 255               

atg gga tct ttc tat caa gaa taa                                        792
Met Gly Ser Phe Tyr Gln Glu                                               
            260                                                           


<210>  56
<211>  263
<212>  PRT
<213>  Ruminococcus gnavus

<400>  56

Met Thr Leu Arg Glu Lys Tyr Gly Glu Trp Gly Ile Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Arg Leu Ala Lys Glu Gly 
            20                  25                  30          


Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Lys Glu Leu 
        35                  40                  45              


Gly Glu Glu Leu Lys Asn Thr Tyr Glu Ile Asp Tyr Lys Val Val Lys 
    50                  55                  60                  


Ala Asp Phe Ser Leu Pro Asp Ala Thr Asp Lys Ile Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Asn Leu Asp Met Gly Phe Met Ala Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Met Lys Cys Phe Tyr His Tyr Met Lys 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Thr Glu Lys 
                165                 170                 175     


Thr Asn Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Thr Ala Gln Thr Pro Glu Glu Val Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ser Gly Glu Arg Asn Lys Ala Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Asp Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Gln Glu 
            260             


