                         SEQUENCE LISTING

<110>  AMSilk GmbH, Ludwig-Maximilians-Universitt Mnchen, Universitt Bayreuth
 
<120>  ARTICLES COMPRISING A SILK POLYPEPTIDE FOR ANTIGEN DELIVERY

<130>  558-86 PCT

<150>  EP 16175724.0
<151>  22.06.2016

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  domain
<222>  (1)..(7)
<223>  Cathepsin S cleavable linker

<400>  1

Gly Pro Met Gly Leu Pro Gly 
1               5           


<210>  2
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  domain
<222>  (1)..(9)
<223>  Cathepsin S amd B cleavable linker

<400>  2

Gly Ala Val Gly Phe Leu Gly Ile Gly 
1               5                   


<210>  3
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic


<220>
<221>  DOMAIN
<222>  (1)..(35)
<223>  Module C (ADF-4)

<400>  3

Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly 
1               5                   10                  15      


Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro 
            20                  25                  30          


Gly Gly Pro 
        35  


<210>  4
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic


<220>
<221>  DOMAIN
<222>  (1)..(35)
<223>  Module Cc

<400>  4

Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly 
1               5                   10                  15      


Tyr Gly Pro Glu Asn Gln Gly Pro Cys Gly Pro Gly Gly Tyr Gly Pro 
            20                  25                  30          


Gly Gly Pro 
        35  


<210>  5
<211>  14
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  domain
<222>  (1)..(14)
<223>  T7-Tag

<400>  5

Gly Cys Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic


<220>
<221>  DOMAIN
<222>  (1)..(8)
<223>  T7-Tag

<400>  6

Gly Gly Cys Gly Gly Ser Gly Gly 
1               5               


<210>  7
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  based on ADF-3


<220>
<221>  DOMAIN
<222>  (1)..(124)
<223>  NR3 (ADF-3)

<400>  7

Gly Ala Ala Ser Ala Ala Val Ser Val Gly Gly Tyr Gly Pro Gln Ser 
1               5                   10                  15      


Ser Ser Ala Pro Val Ala Ser Ala Ala Ala Ser Arg Leu Ser Ser Pro 
            20                  25                  30          


Ala Ala Ser Ser Arg Val Ser Ser Ala Val Ser Ser Leu Val Ser Ser 
        35                  40                  45              


Gly Pro Thr Asn Gln Ala Ala Leu Ser Asn Thr Ile Ser Ser Val Val 
    50                  55                  60                  


Ser Gln Val Ser Ala Ser Asn Pro Gly Leu Ser Gly Cys Asp Val Leu 
65                  70                  75                  80  


Val Gln Ala Leu Leu Glu Val Val Ser Ala Leu Val Ser Ile Leu Gly 
                85                  90                  95      


Ser Ser Ser Ile Gly Gln Ile Asn Tyr Gly Ala Ser Ala Gln Tyr Thr 
            100                 105                 110         


Gln Met Val Gly Gln Ser Val Ala Gln Ala Leu Ala 
        115                 120                 


<210>  8
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  based on ADF-4


<220>
<221>  DOMAIN
<222>  (1)..(109)
<223>  NR4 (ADF-4)

<400>  8

Gly Ala Tyr Gly Pro Ser Pro Ser Ala Ser Ala Ser Val Ala Ala Ser 
1               5                   10                  15      


Arg Leu Ser Ser Pro Ala Ala Ser Ser Arg Val Ser Ser Ala Val Ser 
            20                  25                  30          


Ser Leu Val Ser Ser Gly Pro Thr Asn Gly Ala Ala Val Ser Gly Ala 
        35                  40                  45              


Leu Asn Ser Leu Val Ser Gln Ile Ser Ala Ser Asn Pro Gly Leu Ser 
    50                  55                  60                  


Gly Cys Asp Ala Leu Val Gln Ala Leu Leu Glu Leu Val Ser Ala Leu 
65                  70                  75                  80  


Val Ala Ile Leu Ser Ser Ala Ser Ile Gly Gln Val Asn Val Ser Ser 
                85                  90                  95      


Val Ser Gln Ser Thr Gln Met Ile Ser Gln Ala Leu Ser 
            100                 105                 


<210>  9
<211>  136
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  domain
<222>  (1)..(136)
<223>  derived from Latrodectus hesperus (NR5)

<400>  9

Met Gly Gln Ala Asn Thr Pro Trp Ser Ser Lys Ala Asn Ala Asp Ala 
1               5                   10                  15      


Phe Ile Asn Ser Phe Ile Ser Ala Ala Ser Asn Thr Gly Ser Phe Ser 
            20                  25                  30          


Gln Asp Gln Met Glu Asp Met Ser Leu Ile Gly Asn Thr Leu Met Ala 
        35                  40                  45              


Ala Met Asp Asn Met Gly Gly Arg Ile Thr Pro Ser Lys Leu Gln Ala 
    50                  55                  60                  


Leu Asp Met Ala Phe Ala Ser Ser Val Ala Glu Ile Ala Ala Ser Glu 
65                  70                  75                  80  


Gly Gly Asp Leu Gly Val Thr Thr Asn Ala Ile Ala Asp Ala Leu Thr 
                85                  90                  95      


Ser Ala Phe Tyr Gln Thr Thr Gly Val Val Asn Ser Arg Phe Ile Ser 
            100                 105                 110         


Glu Ile Arg Ser Leu Ile Gly Met Phe Ala Gln Ala Ser Ala Asn Asp 
        115                 120                 125             


Val Tyr Ala Ser Ala Gly Ser Gly 
    130                 135     


<210>  10
<211>  137
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  domain
<222>  (1)..(137)
<223>  derived from Latrodectus hesperus (NR6)

<400>  10

Met Gly Gln Ala Asn Thr Pro Trp Ser Ser Lys Glu Asn Ala Asp Ala 
1               5                   10                  15      


Phe Ile Gly Ala Phe Met Asn Ala Ala Ser Gln Ser Gly Ala Phe Ser 
            20                  25                  30          


Ser Asp Gln Ile Asp Asp Met Ser Val Ile Ser Asn Thr Leu Met Ala 
        35                  40                  45              


Ala Met Asp Asn Met Gly Gly Arg Ile Thr Gln Ser Lys Leu Gln Ala 
    50                  55                  60                  


Leu Asp Met Ala Phe Ala Ser Ser Val Ala Glu Ile Ala Val Ala Asp 
65                  70                  75                  80  


Gly Gln Asn Val Gly Ala Ala Thr Asn Ala Ile Ser Asp Ala Leu Arg 
                85                  90                  95      


Ser Ala Phe Tyr Gln Thr Thr Gly Val Val Asn Asn Gln Phe Ile Thr 
            100                 105                 110         


Gly Ile Ser Ser Leu Ile Gly Met Phe Ala Gln Val Ser Gly Asn Glu 
        115                 120                 125             


Val Ser Tyr Ser Ser Ala Gly Ser Gly 
    130                 135         


<210>  11
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  domain
<222>  (1)..(8)
<223>  epitope of chicken-Ovalbumin

<400>  11

Ser Ile Ile Asn Phe Glu Lys Leu 
1               5               


<210>  12
<211>  576
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  REPEAT
<222>  (1)..(576)
<223>  C16

<400>  12

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg Gly Ser Met Gly 
1               5                   10                  15      


Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr 
            20                  25                  30          


Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly 
        35                  40                  45              


Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro 
    50                  55                  60                  


Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr 
65                  70                  75                  80  


Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala 
                85                  90                  95      


Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro 
            100                 105                 110         


Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala 
        115                 120                 125             


Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro 
    130                 135                 140                 


Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala 
145                 150                 155                 160 


Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn 
                165                 170                 175     


Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser 
            180                 185                 190         


Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly 
        195                 200                 205             


Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly 
    210                 215                 220                 


Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly 
225                 230                 235                 240 


Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly 
                245                 250                 255     


Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser 
            260                 265                 270         


Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly 
        275                 280                 285             


Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala 
    290                 295                 300                 


Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser 
305                 310                 315                 320 


Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala 
                325                 330                 335     


Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln 
            340                 345                 350         


Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser 
        355                 360                 365             


Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro 
    370                 375                 380                 


Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro 
385                 390                 395                 400 


Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly 
                405                 410                 415     


Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro 
            420                 425                 430         


Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly 
        435                 440                 445             


Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly 
    450                 455                 460                 


Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala 
465                 470                 475                 480 


Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly 
                485                 490                 495     


Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala 
            500                 505                 510         


Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly 
        515                 520                 525             


Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala 
    530                 535                 540                 


Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu 
545                 550                 555                 560 


Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly 
                565                 570                 575     


<210>  13
<211>  588
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  REPEAT
<222>  (1)..(585)
<223>  C16-SIINFEKL

<400>  13

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg Gly Ser Met Gly 
1               5                   10                  15      


Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr 
            20                  25                  30          


Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly 
        35                  40                  45              


Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro 
    50                  55                  60                  


Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr 
65                  70                  75                  80  


Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala 
                85                  90                  95      


Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro 
            100                 105                 110         


Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala 
        115                 120                 125             


Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro 
    130                 135                 140                 


Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala 
145                 150                 155                 160 


Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn 
                165                 170                 175     


Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser 
            180                 185                 190         


Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly 
        195                 200                 205             


Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly 
    210                 215                 220                 


Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly 
225                 230                 235                 240 


Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly 
                245                 250                 255     


Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser 
            260                 265                 270         


Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly 
        275                 280                 285             


Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala 
    290                 295                 300                 


Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser 
305                 310                 315                 320 


Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala 
                325                 330                 335     


Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln 
            340                 345                 350         


Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser 
        355                 360                 365             


Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro 
    370                 375                 380                 


Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro 
385                 390                 395                 400 


Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly 
                405                 410                 415     


Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro 
            420                 425                 430         


Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly 
        435                 440                 445             


Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly 
    450                 455                 460                 


Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala 
465                 470                 475                 480 


Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly 
                485                 490                 495     


Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala 
            500                 505                 510         


Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly 
        515                 520                 525             


Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala 
    530                 535                 540                 


Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu 
545                 550                 555                 560 


Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly 
                565                 570                 575     


Gly Ser Gly Ser Ile Ile Asn Phe Glu Lys Leu Gly 
            580                 585             


<210>  14
<211>  591
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  REPEAT
<222>  (1)..(591)
<223>  eADF4(C16-CathS-CD8)

<400>  14

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg Gly Ser Met Gly 
1               5                   10                  15      


Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr 
            20                  25                  30          


Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly 
        35                  40                  45              


Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro 
    50                  55                  60                  


Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr 
65                  70                  75                  80  


Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala 
                85                  90                  95      


Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro 
            100                 105                 110         


Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala 
        115                 120                 125             


Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro 
    130                 135                 140                 


Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala 
145                 150                 155                 160 


Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn 
                165                 170                 175     


Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser 
            180                 185                 190         


Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly 
        195                 200                 205             


Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly 
    210                 215                 220                 


Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly 
225                 230                 235                 240 


Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly 
                245                 250                 255     


Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser 
            260                 265                 270         


Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly 
        275                 280                 285             


Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala 
    290                 295                 300                 


Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser 
305                 310                 315                 320 


Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala 
                325                 330                 335     


Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln 
            340                 345                 350         


Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser 
        355                 360                 365             


Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro 
    370                 375                 380                 


Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro 
385                 390                 395                 400 


Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly 
                405                 410                 415     


Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro 
            420                 425                 430         


Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly 
        435                 440                 445             


Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly 
    450                 455                 460                 


Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala 
465                 470                 475                 480 


Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly 
                485                 490                 495     


Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala 
            500                 505                 510         


Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly 
        515                 520                 525             


Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala 
    530                 535                 540                 


Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu 
545                 550                 555                 560 


Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly 
                565                 570                 575     


Pro Met Gly Leu Pro Gly Ser Ile Ile Asn Phe Glu Lys Leu Gly 
            580                 585                 590     


<210>  15
<211>  593
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  REPEAT
<222>  (1)..(593)
<223>  eADF4(C16-CathB-CD8)

<400>  15

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg Gly Ser Met Gly 
1               5                   10                  15      


Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr 
            20                  25                  30          


Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly 
        35                  40                  45              


Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro 
    50                  55                  60                  


Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr 
65                  70                  75                  80  


Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala 
                85                  90                  95      


Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro 
            100                 105                 110         


Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala 
        115                 120                 125             


Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro 
    130                 135                 140                 


Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala 
145                 150                 155                 160 


Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn 
                165                 170                 175     


Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser 
            180                 185                 190         


Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly 
        195                 200                 205             


Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly 
    210                 215                 220                 


Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly 
225                 230                 235                 240 


Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly 
                245                 250                 255     


Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser 
            260                 265                 270         


Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly 
        275                 280                 285             


Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala 
    290                 295                 300                 


Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser 
305                 310                 315                 320 


Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala 
                325                 330                 335     


Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln 
            340                 345                 350         


Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser 
        355                 360                 365             


Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro 
    370                 375                 380                 


Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro 
385                 390                 395                 400 


Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly 
                405                 410                 415     


Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro 
            420                 425                 430         


Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly 
        435                 440                 445             


Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly Pro Gly Gly 
    450                 455                 460                 


Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala 
465                 470                 475                 480 


Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly Pro Ser Gly 
                485                 490                 495     


Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala Ala 
            500                 505                 510         


Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu Asn Gln Gly 
        515                 520                 525             


Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ser Ser Ala 
    530                 535                 540                 


Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Glu 
545                 550                 555                 560 


Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly 
                565                 570                 575     


Ala Val Gly Phe Leu Gly Ile Gly Ser Ile Ile Asn Phe Glu Lys Leu 
            580                 585                 590         


Gly 
    


<210>  16
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  DOMAIN
<222>  (1)..(11)
<223>  sequence cleaved from the hybrid polypeptide comprising the 
       cathepsin B cleavable linker and the epitope of chicken-Ovalbumin
       (OVA257-264)

<400>  16

Ile Gly Ser Ile Ile Asn Phe Glu Lys Leu Gly 
1               5                   10      


<210>  17
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  DOMAIN
<222>  (1)..(12)
<223>  sequence cleaved from the hybrid polypeptide comprising the 
       cathepsin S cleavable linker and the epitope of chicken-Ovalbumin
       (OVA257-264)

<400>  17

Leu Pro Gly Ser Ile Ile Asn Phe Glu Lys Leu Gly 
1               5                   10          


<210>  18
<211>  35
<212>  PRT
<213>  artificial

<220>
<223>  synthetic


<220>
<221>  DOMAIN
<222>  (1)..(35)
<223>  C kappa

<400>  18

Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly 
1               5                   10                  15      


Tyr Gly Pro Lys Asn Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro 
            20                  25                  30          


Gly Gly Pro 
        35  


<210>  19
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  syntetic


<220>
<221>  DOMAIN
<222>  (1)..(11)
<223>  T7-Tag

<400>  19

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly 
1               5                   10      


<210>  20
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Cathepsin S cleavage site


<220>
<221>  domain
<222>  (1)..(5)

<400>  20

Pro Met Gly Leu Pro 
1               5   


<210>  21
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  Cathepsin B cleavage site


<220>
<221>  domain
<222>  (1)..(4)

<400>  21

Gly Phe Leu Gly 
1               


<210>  22
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  Cathepsin B cleavage site


<220>
<221>  domain
<222>  (1)..(4)

<400>  22

Ala Leu Ala Leu 
1               


<210>  23
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  Cathepsin B cleavage site


<220>
<221>  domain
<222>  (1)..(4)

<400>  23

Gly Gly Gly Phe 
1               


