                         SEQUENCE LISTING

<110>  GENESIS TECHNOLOGIES LIMITED
       AHLFORS, Jan-Eric
       SARATHI, Mani
 
<120>  CRISPR-CAS SYSTEM, MATERIALS AND METHODS

<130>  NWL-008PCT

<150>  US62/351,398
<151>  2016-06-17

<160>  113   

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  1
gagtcgcgcg ctaggggccg ggg                                               23


<210>  2
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  2
ccggggccgg ggccggggcg tgg                                               23


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  3
cccggccccg gccccggccc cgg                                               23


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  4
ggacgtacct aaggcacaag tac                                               23


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  5
ttggagataa tacagcaggt ggg                                               23


<210>  6
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HDR template

<400>  6
ggtgtgaagg actgcatgga ttccatgttc atgagtttgg agataataca gcaggtggct       60

gttgtccct                                                               69


<210>  7
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HDR tempalte

<400>  7
ccacacttcc tgacgtacct aaggtacaag tactcaaacc tctattatgt cgtccaccga       60

caacaggga                                                               69


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  8
ggtatgtcag tcatagttaa gac                                               23


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  9
aaagatagtc atcttggggc tgg                                               23


<210>  10
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HDR template

<400>  10
gaaggtcttc attacacctg cagctctcat tttccataca ttaaagatag tcatcttggg       60

gctcgtcctg ccgctgcttg tcatggtcat ctgctactcg ggaatcct                   108


<210>  11
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HDR template

<400>  11
cttccagaag taatgtggac gtcgagagta aaaggtatgt aatttctatc agtagaaccc       60

cgaccaggac gagga                                                        75


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  12
tgatcttact gattctgaaa tgg                                               23


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  13
cattattttt cattcatagt tgg                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  14
tggccgattc agaattttgt tgg                                               23


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  15
aaattggaag agtttgttta agg                                               23


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  16
cctgtggggc aaagtgaacg tgg                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  17
ttgacagcag tcttctccac agg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  18
aacgctacac gtttcgtgtt tgg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  19
ctgcccatcc acactaggca agg                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  20
cagcaaccag acggacaagc tgg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  21
cacgaagctc tccgatgtgt tgg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequenc

<400>  22
tatctcatac ctatccctat tgg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  23
acaactgggg ccaggtgcgg tgg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  24
caatttggtc agctgggtct tgg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  25
tatacacagc attctctgaa agg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  26
ttaaacatta acggaacccc cgg                                               23


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  27
tggcgtgatc tgcgcgcccc agg                                               23


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  28
ctctctgagg taacaaattg ggg                                               23


<210>  29
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  29
tctcagagag gagtaaacac tgg                                               23


<210>  30
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  30
tcgaggagat ctcgaataga agg                                               23


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  31
cgcctctgct ctgtatcggg agg                                               23


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  32
caacgatctg accgccaccc ggg                                               23


<210>  33
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  33
gccgcgcagg ggccctagat tgg                                               23


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  34
cagaattgat actgactgta tgg                                               23


<210>  35
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  35
aaagatagtc atcttggggc tgg                                               23


<210>  36
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  36
cccctatctt tattgtgacg agg                                               23


<210>  37
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  37
aaggaagctc tattagatac agg                                               23


<210>  38
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  38
cccaagacac accatcgatc tgg                                               23


<210>  39
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  39
caataacttt gcaacagtga agg                                               23


<210>  40
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  40
agatgccagc agatcagctc agg                                               23


<210>  41
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  41
ctcagctttt tctcactcta tgg                                               23


<210>  42
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  42
caacgtgctg gtctgagtgc tgg                                               23


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  43
agctgtggga ggaagataag agg                                               23


<210>  44
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  44
agcctccacc agctcctcct cgg                                               23


<210>  45
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  45
atgcaggcat cctcagctac agg                                               23


<210>  46
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  46
gctaggccac gccgaggtcc tgg                                               23


<210>  47
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  47
agttatggcg acgaaggccg tgg                                               23


<210>  48
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  48
catgaacacg gaatccatgc agg                                               23


<210>  49
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  49
ttggagataa tacagcaggt ggg                                               23


<210>  50
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  50
gagtcgcgcg ctaggggccg ggg                                               23


<210>  51
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  51
ccggggccgg ggccggggcg tgg                                               23


<210>  52
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  52
cccggccccg gccccggccc cgg                                               23


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  53
tgccgcacgc cccctggcag cgg                                               23


<210>  54
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  54
ggcggcggag gcggcggcgg cgg                                               23


<210>  55
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  55
ccaggcccag gcccaggccc agg                                               23


<210>  56
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  56
cctgcgcctg ccctgggaac ggg                                               23


<210>  57
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  57
cctgggcctg ggcctgggcc tgg                                               23


<210>  58
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  58
ccatgtgtac tacatccaca cgg                                               23


<210>  59
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  59
aaactcactg gagtttgacg tgg                                               23


<210>  60
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  60
agcggtaact aagattagta tgg                                               23


<210>  61
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  61
ttccaactgt tcatcggctg agg                                               23


<210>  62
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  62
gtccgtgctg tccgcctcgg agg                                               23


<210>  63
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  63
tggggccgca gggcgtggat ggg                                               23


<210>  64
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  64
cataaatttt acatttgagt tgg                                               23


<210>  65
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  65
gatgatataa caaatcaata tgg                                               23


<210>  66
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  66
gagattgatt ttctaataaa cgg                                               23


<210>  67
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  67
ccgccgatat attacagaac agg                                               23


<210>  68
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  68
accccgcctg taacacgagc agg                                               23


<210>  69
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  69
taccacagag tctagactcg tgg                                               23


<210>  70
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  70
gtttatgttg aatagcattg agg                                               23


<210>  71
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  71
aaccgagtcc gatgaaaaaa agg                                               23


<210>  72
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  72
gcttcgggcg cttcttgcag agg                                               23


<210>  73
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  73
ctgggggcag ccgatacccg ggg                                               23


<210>  74
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  74
ccaggatcca actgcctatg tgg                                               23


<210>  75
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  75
tgtggccatg tggagtgacg tgg                                               23


<210>  76
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  76
catctaattc aacaagaatt ggg                                               23


<210>  77
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  77
gggcaaaaat tctctgtcag tgg                                               23


<210>  78
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  78
ccctcctatt accgaggctg tgg                                               23


<210>  79
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  79
ttgatactcc tgggacaaat ggg                                               23


<210>  80
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  80
ggtatgatta gaatcaatgg cga                                               23


<210>  81
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  81
ttccaactgt tcatcggctg agg                                               23


<210>  82
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  82
atatcttcct aattactatg cttatcttgg ttaccgctaa caacctattc caactgttca       60

tcggatggga gggcgtagga tcat                                              84


<210>  83
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HDR template

<400>  83
tatagaagga ttaatgatac gaatagaacc aatggcgatt gttggataag gttgacaagt       60

agcctaccct cccgcatcct agta                                              84


<210>  84
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  84
ggtcctggag ccgcaccgga tcg                                               23


<210>  85
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  85
agttatggcg acgaaggccg tgg                                               23


<210>  86
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  86
ggaggctccg cctgtcgtgc ctg                                               23


<210>  87
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  87
tggggccgca gggcgtggat ggg                                               23


<210>  88
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  88
ggttgagttt acattttaaa tac                                               23


<210>  89
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  89
gatgatataa caaatcaata tgg                                               23


<210>  90
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  90
ggcaaataat cttttagtta gag                                               23


<210>  91
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  91
ccgccgatat attacagaac agg                                               23


<210>  92
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  92
ggacgagcac aatgtccgcc cca                                               23


<210>  93
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  93
taccacagag tctagactcg tgg                                               23


<210>  94
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  94
ggagttacga taagttgtat ttg                                               23


<210>  95
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  95
aaccgagtcc gatgaaaaaa agg                                               23


<210>  96
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  96
ggagacgttc ttcgcgggct tcg                                               23


<210>  97
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  97
ctgggggcag ccgatacccg ggg                                               23


<210>  98
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  98
gggttaagaa caacttaatc tac                                               23


<210>  99
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  99
gggcaaaaat tctctgtcag tgg                                               23


<210>  100
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  100
ggtgtcggag ccattatcct ccc                                               23


<210>  101
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  101
ttgatactcc tgggacaaat ggg                                               23


<210>  102
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  102
ggtctagcta ccacacagaa ccc                                               23


<210>  103
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  103
caataacttt gcaacagtga agg                                               23


<210>  104
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  104
gggtctagca agagcaggtg tgggtttagg aggtgtgtg                              39


<210>  105
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  105
gccccgacca cgccccggcc ccggccccgg cccctagcg                              39


<210>  106
<211>  7669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid sequence

<400>  106
gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca       60

gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc      120

ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca      180

acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg      240

ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg      300

ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg      360

ggtttcacca tattggccag gctggtgtgc aactcctaat ctcaggtgat ctacccacct      420

tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttcct      480

gcaggaagct tccgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg      540

ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc      600

gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac      660

aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt      720

tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag      780

aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg      840

aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa      900

tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc      960

aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag     1020

tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa     1080

ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc     1140

taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg     1200

agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa     1260

caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa     1320

tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg     1380

gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag     1440

cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg     1500

caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt     1560

ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt     1620

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     1680

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1740

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1800

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1860

gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga     1920

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1980

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     2040

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     2100

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     2160

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     2220

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2280

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2340

cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga caagcttcct     2400

aggtatcccg gggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2460

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2520

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2580

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2640

aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg     2700

tgccttgaat tacttccacg cccctggctg cagtacgtga ttcttgatcc cgagcttcgg     2760

gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct     2820

tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc     2880

gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg actcactgcg     2940

acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt     3000

tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg     3060

aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg     3120

cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg     3180

gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg     3240

agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg     3300

aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg     3360

tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag     3420

gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct     3480

tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt     3540

ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagccggc     3600

tggggagggg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc     3660

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag     3720

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc     3780

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac     3840

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag     3900

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc     3960

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc     4020

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc     4080

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc     4140

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg     4200

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag     4260

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac     4320

gagctgtaca agtccggact cagatctaaa taaacttata aattgtgaga gaaattaatg     4380

aatgtctaag ttaatgcaga aacggagaga catactatat tcatgaacta aaagacttaa     4440

tattgtgaag gtatactttc tttccacata aatttgtagt caatatgttc accccaaaaa     4500

agctgtttgt taacttgcca acctcattct aaaatgtata tagaagccca aaagacaata     4560

acaaaaatat tcttgtagaa caaaatggga aagaatgttc cactaaatat caagatttag     4620

agcaaagcat gagatgtgtg gggatagaca gtgaggctga taaaatagag tagagctcag     4680

aaacagaccc attgatatat gtaagtgacc tatgaaaaaa atatggcatt ttacaatggg     4740

aaaatgatga tctttttctt ttttagaaaa acagggaaat atatttatat gtaaaaaata     4800

aaagggaacc catatgtcat accatacaca caaaaaaatt ccagtgaatt ataagtctaa     4860

atggagaagg caaaacttta aatcttttag aaaataatat agaagcatgc catcatgact     4920

tcagtgtaga gaaaaatttc ttatgactca aagtcctaac cacaaagaaa agattgttaa     4980

ttagattgca tgaatattaa gacttatttt taaaattaaa aaaccattaa gaaaagtcag     5040

gccatagaat gacagaaaat atttgcaaca ccccagtaaa gagaattgta atatgcagat     5100

tataaaaaga agtcttacaa atcagtaaaa aataaaacta gacaaaaatt tgaacagatg     5160

aaagagaaac tctaaataat cattacacat gagaaactca atctcagaaa tcagagaact     5220

atcattgcat atacactaaa ttagagaaat attaaaaggc taagtaacat ctgtggcaat     5280

attgatggta tataaccttg atatgatgtg atgagaacag tactttaccc catgggcttc     5340

ctccccaaac ccttacccca gtataaatca tgacaaatat actttaaaaa ccattaccct     5400

atatctaacc agtactcctc aaaactgtca aggtcatcaa aaataagaaa agtctgagga     5460

actgtcaaaa ctaagaggaa cccaaggaga catgagaatt atatgtaatg tggcattctg     5520

aatgagatcc cagaacagaa aaagaacagt agctaaaaaa ctaatgaaat ataaataaag     5580

tttgaacttt agtttttttt aaaaaagagt agcattaaca cggcaaagcc attttcatat     5640

ttttcttgaa cattaagtac aagtctataa ttaaaaattt tttaaatgta gtctggaaca     5700

ttgccagaaa cagaagtaca gcagctatct gtgctgtcgc ctaactatcc atagctgatt     5760

ggtctaaaat gagatacatc aacgctcctc catgtttttt gttttctttt taaatgaaaa     5820

actttatttt ttaagaggag tttcaggttc atagcaaaat tgagaggaag gtacattcaa     5880

gctgaggaag ttttcctcta ttcctagttt actgagagat tgcatcatga atgggtgtta     5940

aattttgtca aatgcttttt ctgtgtctat caatatgacc atgtgatttt cttctttaac     6000

ctgttgatgg gacaaattac gttaattgat tttcaaacgt tgaaccaccc ttacatatct     6060

ggaataaatt ctacttggtt gtggtgtata ttttttgata cattcttgga ttctttttgc     6120

taatattttg ttgaaaatgt ttgtatcttt gttcatgaga gatattggtc tgttgttttc     6180

ttttcttgta atgtcatttt ctagttccgg tattaaggta atgctggcct agttgaatga     6240

tttaggaagt attccctctg cttctgtctt ctgaaagaga ttgtagaaag ttgatacaat     6300

ttttttttct ttaaatatct tgatagaatt ctgcagtcga cggtaccgcg ggcccgggat     6360

ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg     6420

ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg     6480

ttgttggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta     6540

aaagaaaagg ggggacaccg gtaattcgaa cgctgacgtc atcaacccgc tccaaggaat     6600

cgcgggccca gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg     6660

gctgtgaggg acaggggagt ggcgccctgc aatatttgca tgtcgctatg tgttctggga     6720

aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct gtatgagaca     6780

ccttggatcc gagtcgcgcg ctaggggccg gttttagagc tagaaatagc aagttaaaat     6840

aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgaattcg     6900

agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga     6960

taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa     7020

agtaataatt tcttgtgtag tttgcagttt taaaattatg ttttaaaatg gactatcata     7080

tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac     7140

gaaacaccgc cggggccggg gccggggcgg ttttagagct agaaatagca agttaaaata     7200

aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgaattcga     7260

acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca     7320

cccagcgcac gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg     7380

caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga     7440

tttgggaatc ttataagttc tgtatgagac accttggatc ccccggcccc ggccccggcc     7500

cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag     7560

tggcaccgag tcggtgcttt ttttgaattc gtagtagttc atgtcatctt attattcagt     7620

atttataact tgcaaagaaa tgaatatcag agagtgagag gggcgcgcc                 7669


<210>  107
<211>  7345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid sequence

<400>  107
gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca       60

gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc      120

ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca      180

acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg      240

ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg      300

ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg      360

ggtttcacca tattggccag gctggtgtgc aactcctaat ctcaggtgat ctacccacct      420

tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttcct      480

gcaggaagct tccgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg      540

ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc      600

gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac      660

aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt      720

tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag      780

aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg      840

aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa      900

tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc      960

aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag     1020

tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa     1080

ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc     1140

taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg     1200

agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa     1260

caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa     1320

tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg     1380

gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag     1440

cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg     1500

caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt     1560

ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt     1620

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     1680

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1740

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1800

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1860

gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga     1920

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1980

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     2040

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     2100

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     2160

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     2220

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2280

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2340

cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga caagcttcct     2400

aggtatcccg gggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2460

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2520

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2580

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2640

aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg     2700

tgccttgaat tacttccacg cccctggctg cagtacgtga ttcttgatcc cgagcttcgg     2760

gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct     2820

tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc     2880

gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg actcactgcg     2940

acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt     3000

tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg     3060

aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg     3120

cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg     3180

gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg     3240

agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg     3300

aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg     3360

tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag     3420

gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct     3480

tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt     3540

ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagccggc     3600

tggggagggg ctagcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg     3660

cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag     3720

ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag     3780

ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc     3840

cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac     3900

gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg     3960

aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag     4020

gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc     4080

atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag     4140

gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc     4200

gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac     4260

gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc     4320

atggacgagc tgtacaagtc cggactcaga tctaaataaa cttataaatt gtgagagaaa     4380

ttaatgaatg tctaagttaa tgcagaaacg gagagacata ctatattcat gaactaaaag     4440

acttaatatt gtgaaggtat actttctttc cacataaatt tgtagtcaat atgttcaccc     4500

caaaaaagct gtttgttaac ttgccaacct cattctaaaa tgtatataga agcccaaaag     4560

acaataacaa aaatattctt gtagaacaaa atgggaaaga atgttccact aaatatcaag     4620

atttagagca aagcatgaga tgtgtgggga tagacagtga ggctgataaa atagagtaga     4680

gctcagaaac agacccattg atatatgtaa gtgacctatg aaaaaaatat ggcattttac     4740

aatgggaaaa tgatgatctt tttctttttt agaaaaacag ggaaatatat ttatatgtaa     4800

aaaataaaag ggaacccata tgtcatacca tacacacaaa aaaattccag tgaattataa     4860

gtctaaatgg agaaggcaaa actttaaatc ttttagaaaa taatatagaa gcatgccatc     4920

atgacttcag tgtagagaaa aatttcttat gactcaaagt cctaaccaca aagaaaagat     4980

tgttaattag attgcatgaa tattaagact tatttttaaa attaaaaaac cattaagaaa     5040

agtcaggcca tagaatgaca gaaaatattt gcaacacccc agtaaagaga attgtaatat     5100

gcagattata aaaagaagtc ttacaaatca gtaaaaaata aaactagaca aaaatttgaa     5160

cagatgaaag agaaactcta aataatcatt acacatgaga aactcaatct cagaaatcag     5220

agaactatca ttgcatatac actaaattag agaaatatta aaaggctaag taacatctgt     5280

ggcaatattg atggtatata accttgatat gatgtgatga gaacagtact ttaccccatg     5340

ggcttcctcc ccaaaccctt accccagtat aaatcatgac aaatatactt taaaaaccat     5400

taccctatat ctaaccagta ctcctcaaaa ctgtcaaggt catcaaaaat aagaaaagtc     5460

tgaggaactg tcaaaactaa gaggaaccca aggagacatg agaattatat gtaatgtggc     5520

attctgaatg agatcccaga acagaaaaag aacagtagct aaaaaactaa tgaaatataa     5580

ataaagtttg aactttagtt ttttttaaaa aagagtagca ttaacacggc aaagccattt     5640

tcatattttt cttgaacatt aagtacaagt ctataattaa aaatttttta aatgtagtct     5700

ggaacattgc cagaaacaga agtacagcag ctatctgtgc tgtcgcctaa ctatccatag     5760

ctgattggtc taaaatgaga tacatcaacg ctcctccatg ttttttgttt tctttttaaa     5820

tgaaaaactt tattttttaa gaggagtttc aggttcatag caaaattgag aggaaggtac     5880

attcaagctg aggaagtttt cctctattcc tagtttactg agagattgca tcatgaatgg     5940

gtgttaaatt ttgtcaaatg ctttttctgt gtctatcaat atgaccatgt gattttcttc     6000

tttaacctgt tgatgggaca aattacgtta attgattttc aaacgttgaa ccacccttac     6060

atatctggaa taaattctac ttggttgtgg tgtatatttt ttgatacatt cttggattct     6120

ttttgctaat attttgttga aaatgtttgt atctttgttc atgagagata ttggtctgtt     6180

gttttctttt cttgtaatgt cattttctag ttccggtatt aaggtaatgc tggcctagtt     6240

gaatgattta ggaagtattc cctctgcttc tgtcttctga aagagattgt agaaagttga     6300

tacaattttt ttttctttaa atatcttgat agaattctgc agtcgacggt accgcgggcc     6360

cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt     6420

tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa     6480

ttgttgttgt tactagtttt aagaccaatg acttacaagg cagctgtaga tcttagccac     6540

tttttaaaag aaaagggggg acaccggtaa ttcgaacgct gacgtcatca acccgctcca     6600

aggaatcgcg ggcccagtgt cactaggcgg gaacacccag cgcgcgtgcg ccctggcagg     6660

aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc gctatgtgtt     6720

ctgggaaatc accataaacg tgaaatgtct ttggatttgg gaatcttata agttctgtat     6780

gagacacctt ggatccggca ttcgtggatg gcgatcgttt tagagctaga aatagcaagt     6840

taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg     6900

aattcgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta     6960

gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac     7020

gtagaaagta ataatttctt gtgtagtttg cagttttaaa attatgtttt aaaatggact     7080

atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga     7140

aaggacgaaa caccgaccaa ttcagggacc agcgcgtttt agagctagaa atagcaagtt     7200

aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttga     7260

attcgtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata     7320

tctcagagag tgagagggcg gccgc                                           7345


<210>  108
<211>  6747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid sequence

<400>  108
gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca       60

gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc      120

ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca      180

acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg      240

ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg      300

ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg      360

ggtttcacca tattggccag gctggtgtgc aactcctaat ctcaggtgat ctacccacct      420

tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttcct      480

gcaggaagct tccgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg      540

ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc      600

gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac      660

aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt      720

tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag      780

aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg      840

aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa      900

tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc      960

aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag     1020

tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa     1080

ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc     1140

taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg     1200

agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa     1260

caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa     1320

tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg     1380

gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag     1440

cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg     1500

caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt     1560

ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt     1620

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     1680

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1740

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1800

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1860

gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga     1920

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1980

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     2040

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     2100

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     2160

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     2220

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2280

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2340

cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga caagcttcct     2400

aggtatcccg gggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg     2460

agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa     2520

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt     2580

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac     2640

aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg     2700

tgccttgaat tacttccacg cccctggctg cagtacgtga ttcttgatcc cgagcttcgg     2760

gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct     2820

tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc     2880

gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg actcactgcg     2940

acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt     3000

tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg     3060

aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg     3120

cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg     3180

gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg     3240

agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg     3300

aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg     3360

tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag     3420

gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct     3480

tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt     3540

ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagccggc     3600

tggggaggga cgcgtgccac catgaaccgg ggagtccctt ttaggcactt gcttctggtg     3660

ctgcaactgg cgctcctccc agcagccact cagggaaaga aagtggtgct gggcaaaaaa     3720

ggggatacag tggaactgac ctgtacagct tcccagaaga agagcataca attccactgg     3780

aaaaactcca accagataaa gattctggga aatcagggct ccttcttaac taaaggtcca     3840

tccaagctga atgatcgcgc tgactcaaga agaagccttt gggaccaagg aaacttcccc     3900

ctgatcatca agaatcttaa gatagaagac tcagatactt acatctgtga agtggaggac     3960

cagaaggagg aggtgcaatt gctagtgttc ggattgactg ccaactctga cacccacctg     4020

cttcaggggc agagcctgac cctgaccttg gagagccccc ctggtagtag cccctcagtg     4080

caatgtagga gtccaagggg taaaaacata caggggggga agaccctctc cgtgtctcag     4140

ctggagctcc aggatagtgg cacctggaca tgcactgtct tgcagaacca gaagaaggtg     4200

gagttcaaaa tagacatcgt ggtgctagct ttccagaagg cctccagcat agtctataag     4260

aaagaggggg aacaggtgga gttctccttc ccactcgcct ttacagttga aaagctgacg     4320

ggcagtggcg agctgtggtg gcaggcggag agggcttcct cctccaagtc ttggatcacc     4380

tttgacctga agaacaagga agtgtctgta aaacgggtta cccaggaccc taagctccag     4440

atgggcaaga agctcccgct ccacctcacc ctgccccagg ccttgcctca gtatgctggc     4500

tctggaaacc tcaccctggc ccttgaagcg aaaacaggaa agttgcatca ggaagtgaac     4560

ctggtggtga tgagagccac tcagctccag aaaaatttga cctgtgaggt gtggggaccc     4620

acctccccta agctgatgct gagcttgaaa ctggagaaca aggaggcaaa ggtctcgaag     4680

cgggagaagg cggtgtgggt gctgaaccct gaggcgggga tgtggcagtg tctgctgagt     4740

gactcgggac aggtcctgct ggaatccaac atcaaggttc tgcccacatg gtcgaccccg     4800

gtgcagccaa tggccctgat tgtgctgggg ggcgtcgccg gcctcctgct tttcattggg     4860

ctaggcatct tcttctgtgt caggtgccgg cactccggac tcagatctaa atgaactagt     4920

gaagttccta ttctctagaa agtataggaa cttcacttca ttttccgtct tcgagggcct     4980

atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattgg     5040

aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata     5100

atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac     5160

cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac     5220

cgcaccccaa aggtgaccgt ccgttttaga gctagaaata gcaagttaaa ataaggctag     5280

tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgctccg cggcacgaga     5340

actcaaagcc ccggggcctg ggtcccacgc ggggtccctt acccagggtg ccccgggcgc     5400

tcatttgcat gtcccaccca acaggtaaac ctgacaggtc atcgcggcca ggtacgacct     5460

ggcggtcaga gcaccaaaca tacgagcctt gtgatgagtt ccgttgcatg aaattctccc     5520

aaaggctcca agatggacag gaaagggcgc ggttcggtca ccgtaagtag aataggtgaa     5580

agactcccgt gccttataag gcctgtgggt gacttcttct caccgaagtg tgatcacttg     5640

ggtgggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa     5700

aaagtggcac cgagtcggtg cttttttgaa gacacaccgc tctcaatatt ggccattagc     5760

catattattc attggttata tagcataaat caatattggc tattggccat tgcatacgtt     5820

gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg     5880

gcattgatta ttggtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc     5940

actttttaaa agaaaagggg ggacaccggt aattcgaacg ctgacgtcat caacccgctc     6000

caaggaatcg cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca     6060

ggaagatggc tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg     6120

ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt gggaatctta taagttctgt     6180

atgagacacc ttggatccag atgactatct ttaatgtcgt tttagagcta gaaatagcaa     6240

gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt     6300

tgaattcgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt     6360

tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg     6420

acgtagaaag taataatttc ttgtgtagtt tgcagtttta aaattatgtt ttaaaatgga     6480

ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg     6540

gaaaggacga aacaccgggt cctgccgctg cttgtcagtt ttagagctag aaatagcaag     6600

ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt     6660

gaattcgtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa     6720

tatctcagag agtgagaggg cggccgc                                         6747


<210>  109
<211>  4900
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cas9n mRNA

<400>  109
auggcuccca agaagaagcg aaaggugggc auccacggcg ugcccgcugc cgacaaaaag       60

uauaguaucg gacuggcuau uggcacuaac agcgugggau gggccgucau caccgacgag      120

uacaaagugc caagcaagaa guucaagguc cugggaaaca ccgauagaca caguaucaag      180

aaaaaucuga uuggagcccu gcuguucgac ucaggggaga cagcugaagc aacuaggcug      240

aaaagaacag cuaggagacg guauacucgc cgaaagaauc ggaucugcua ccuccaggag      300

auuuucucca acgaaauggc caagguggac gauaguuucu uucaucgccu ggaggaauca      360

uuccuggucg aggaagauaa gaaacacgag aggcauccca ucuuuggcaa cauuguggac      420

gaggucgcuu aucacgaaaa guacccuaca aucuaucauc ugcggaagaa acugguggac      480

agcacugaua aggcagaccu gcgccugauc uaucuggccc uggcucacau gauuaaguuc      540

agggggcauu uucugaucga gggcgaucug aacccagaca auuccgaugu ggacaagcug      600

uucauccagc ugguccagac auacaaucag cuguuugagg aaaaccccau uaaugcaucu      660

gggguggacg caaaagccau ccugagugcc agacugucua agaguaggag acuggagaac      720

cugaucgcuc agcugccagg cgaaaagaaa aacggccugu uuggaaaucu gauugcacug      780

ucacugggac ugacccccaa cuucaagagc aauuuugauc uggccgagga cgcuaagcuc      840

cagcugagca aggacaccua cgacgaugac cuggauaacc ugcuggcuca gaucggcgau      900

caguacgcag accuguuccu ggccgcuaag aaucugucug acgccauccu gcugagugau      960

auucugagag ugaacaccga gauuacaaaa gccccccugu cagcuagcau gaucaagaga     1020

uaugacgagc accaucagga ucugacccug cugaaggcuc uggugcggca gcagcugccu     1080

gagaaguaca aagaaaucuu cuuugaucag agcaagaaug gguacgccgg cuauauugac     1140

ggcggagcuu cccaggagga guucuacaag uuuaucaaac cuauucugga gaagauggac     1200

ggcacugagg aacugcuggu gaaacugaau cgggaagacc ugcugcggaa gcagcgcacc     1260

uucgauaacg gcagcauccc ucaccagauu caucugggag agcugcacgc aauccugcgg     1320

cgccaggaag acuucuaccc auuucugaag gauaaccggg agaagaucga aaaaauucug     1380

acuuuccgca uccccuacua uguggggccu cuggcaagag gcaauucccg guuugccugg     1440

augacccgca agucugagga aacaaucacu cccuggaacu ucgaggaagu ggucgauaag     1500

ggcgcuuccg cacagucuuu cauugagagg augacaaauu uugacaagaa ccugccaaau     1560

gaaaaagugc ugcccaagca cagccugcug uacgaguauu ucaccgucua uaacgaacug     1620

acaaagguga aauacgucac ugagggcaug agaaagccug ccuuccuguc cggagaacag     1680

aagaaagcua ucguggaccu gcuguuuaaa accaaucgga aggugacagu caagcagcug     1740

aaagaggacu acuucaagaa aauugaaugu uucgauucug uggagaucag uggggucgaa     1800

gacagguuua acgccucucu gggcaccuac cacgaucugc ugaagaucau uaaggauaaa     1860

gacuuccugg acaacgagga aaaugaggac auccuggagg acauugugcu gacccugaca     1920

cuguuugagg aucgggaaau gaucgaggaa cgccugaaga ccuacgccca ucuguucgau     1980

gacaaaguga ugaaacagcu gaagcgaagg agauacacug gguggggccg acugagcagg     2040

aagcugauca auggcauucg cgacaaacag aguggaaaga caauccugga cuuucugaag     2100

ucagauggcu ucgcuaacag gaauuuuaug cagcugauuc acgaugacuc ucugacuuuc     2160

aaagaggaca uccagaaggc acaggugucc ggacaggggg acucucugca cgagcauauc     2220

gcaaaccugg ccgggagccc ugccaucaag aaaggcaucc uccagaccgu gaagguggug     2280

gacgagcugg ugaaagucau gggaagacau aagccagaaa acaucgugau ugagauggcc     2340

agggagaauc agaccacaca gaaagggcag aagaacucuc gggagcgcau gaaacgcauc     2400

gaggaaggaa uuaaggaacu ggggagucag auccugaaag agcaccccgu ggaaaacaca     2460

cagcuccaga augagaagcu guaucuguac uaccuccaga auggccgcga uauguacgug     2520

gaccaggagc uggauauuaa ccgacuguca gauuaugacg uggaucauau cgucccacag     2580

ucauuccuga aagaugacag cauugacaau aaggugcuga cccgcagcga caaaaaccga     2640

ggaaagagug auaauguccc cucagaggaa guggucaaga aaaugaagaa cuacuggagg     2700

cagcugcuga augccaaacu gaucacccag cgaaaguuug auaaccugac aaaagcugag     2760

agggggggcc uguccgaacu ggacaaagca ggcuucauca agcgacagcu gguggagaca     2820

aggcagauca caaagcacgu cgcucagauc cuggacagca ggaugaacac caaguacgau     2880

gagaaugaca aacugauccg ggaagugaag gucauuacac ugaagucaaa acuggugagc     2940

gacuuuagga aagauuucca guucuacaag gucagagaga ucaacaacua ccaccaugcu     3000

caugacgcau accugaacgc aguggucggg acugcccuga uuaagaaaua cccuaaacug     3060

gagucugagu ucguguacgg cgacuauaag guguacgaug ucagaaaaau gaucgccaag     3120

agcgagcagg aaauuggcaa agccaccgcu aaguauuucu uuuacuccaa caucaugaau     3180

uucuuuaaga cugagaucac ccuggcaaau ggcgaaaucc gaaagaggcc acugauugag     3240

acuaacggag agacagggga aaucgugugg gacaaaggaa gagauuuugc uaccgugcgg     3300

aagguccuga guaugcccca agugaauauu gucaagaaaa cagaggugca gacuggaggg     3360

uucaguaagg aaucaauucu gccuaaacgc aacagcgaua agcugaucgc ccgaaagaaa     3420

gacugggacc ccaagaagua uggcggauuc gacuccccaa ccguggcuua cucuguccug     3480

guggucgcaa agguggagaa gggaaaaagc aagaaacuga aauccgucaa ggaacugcug     3540

gggaucacaa uuauggagag gagcagcuuc gaaaagaauc cuaucgauuu ucuggaggcc     3600

aaaggguaua aggaagugaa gaaagaccug aucaucaagc ugccaaagua cucucuguuu     3660

gagcuggaaa acggcagaaa gcggaugcug gcaagugccg gcgagcugca aaaaggaaau     3720

gaacuggccc ugcccucaaa guacgugaac uuccuguauc uggcuagcca cuacgagaag     3780

cugaaaggcu ccccugagga uaacgaacag aaacagcugu uuguggagca gcacaagcau     3840

uaucuggacg agaucauuga acagauuagc gaguucucca aacgcgugau ccuggcugac     3900

gcaaaucugg auaagguccu gucugcauac aacaaacaca gggacaagcc aaucagagag     3960

caggccgaaa auaucauuca ucuguucacu cugaccaacc ugggagcccc cgcagccuuc     4020

aaguauuuug acacuaccau cgaucgcaaa cgauacacaa gcacuaagga ggugcuggau     4080

gcuacccuga uccaccagag cauuacuggg cuguacgaga caaggaucga ccugucccag     4140

cuggggggag acaaacgccc agccgccacc aagaaagcag gacaggcaaa gaagaagaag     4200

uaggcggccg cgaauaugau cagguaacuu ucgaccgacu uuccccaaga gaaaauuccu     4260

agaaauugaa caaaaauguu uccacuggcu uuugccugua agaaaaaaaa uguacccgag     4320

cacauagagc uuuuuaauag cacuaaccaa ugccuuuuua gauguauuuu ugauguauau     4380

aucuauuauu caaaaaauca uguuuauuuu gaguccuagg acuuaaaauu agucuuuugu     4440

aauaucaagc aggacccuaa gaugaagcug agcuuuugau gccaggugca aucuacugga     4500

aauguagcac uuacguaaaa cauuuguuuc ccccacaguu uuaauaagaa cagaucagga     4560

auucuaaaua aauuucccag uuaaagauua uugugacuuc acuguauaua aacauauuuu     4620

uauacuuuau ugaaagggga caccucuaua uucuuccauc aucacuguaa agacaaauaa     4680

augauuauau ucacaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4740

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4800

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4860

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa                           4900


<210>  110
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  110
atactgtggc cacaagcatg                                                   20


<210>  111
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  111
gttccccatc ctaaatgtgg                                                   20


<210>  112
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  112
ggcattcgtg gatggcgatc                                                   20


<210>  113
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA sequence

<400>  113
accaattcag ggaccagcgc                                                   20


