                         SEQUENCE LISTING

<110>  Autolus Ltd
 
<120>  CELL

<130>  P109950PCT

<150>  GB 1610515.7
<151>  2016-06-16

<160>  71    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region (VH) complementarity determining 
       region (CDR) CDR1

<400>  1

Ser Tyr Trp Met Asn 
1               5   


<210>  2
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region (VH) CDR2

<400>  2

Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 
1               5                   10                  15      


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region (VH) CDR3

<400>  3

Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp Tyr 
1               5                   10                  15  


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region (VL) CDR1

<400>  4

Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr Leu Asn 
1               5                   10                  15  


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region (VL) CDR2

<400>  5

Asp Ala Ser Asn Leu Val Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region (VL) CDR3

<400>  6

Gln Gln Ser Thr Glu Asp Pro Trp Thr 
1               5                   


<210>  7
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric antigen receptor (CAR), Murine CD19ALAb VH sequence

<400>  7

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 


<210>  8
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Humanised CD19ALAb VH sequence

<400>  8

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Glu Ser Ala Arg Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Gly Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
            100                 105                 110         


Tyr Trp Gly Lys Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  9
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Murine CD19ALAb VL sequence

<400>  9

Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile Pro Pro 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln Gln Ser Thr 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  10
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Humanised CD19ALAb VL sequence, Kappa 16

<400>  10

Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Ala Asp Val Ala Val Tyr His Cys Gln Gln Ser Thr 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  11
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Humanised CD19ALAb VL sequence, Kappa 7

<400>  11

Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Val Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Ala Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Thr 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110         


<210>  12
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Murine CD19ALAb scFv sequence

<400>  12

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Asp Ile Gln Leu 
        115                 120                 125             


Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr 
    130                 135                 140                 


Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr 
145                 150                 155                 160 


Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln Pro Pro Lys Leu Leu Ile 
                165                 170                 175     


Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile Pro Pro Arg Phe Ser Gly 
            180                 185                 190         


Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His Pro Val Glu Lys 
        195                 200                 205             


Val Asp Ala Ala Thr Tyr His Cys Gln Gln Ser Thr Glu Asp Pro Trp 
    210                 215                 220                 


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
225                 230                 235 


<210>  13
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Humanised CD19ALAb scFv sequence - Heavy 19, Kappa 16

<400>  13

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Glu Ser Ala Arg Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Gly Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
            100                 105                 110         


Tyr Trp Gly Lys Gly Thr Leu Val Thr Val Ser Ser Asp Ile Gln Leu 
        115                 120                 125             


Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr 
    130                 135                 140                 


Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr 
145                 150                 155                 160 


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 
                165                 170                 175     


Tyr Asp Ala Ser Asn Leu Val Ser Gly Val Pro Asp Arg Phe Ser Gly 
            180                 185                 190         


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 
        195                 200                 205             


Ala Asp Val Ala Val Tyr His Cys Gln Gln Ser Thr Glu Asp Pro Trp 
    210                 215                 220                 


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
225                 230                 235     


<210>  14
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Humanised CD19ALAb scFv sequence - Heavy 19, Kappa 7

<400>  14

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ala Pro Gly Gln Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Glu Ser Ala Arg Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Gly Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
            100                 105                 110         


Tyr Trp Gly Lys Gly Thr Leu Val Thr Val Ser Ser Asp Ile Gln Leu 
        115                 120                 125             


Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr 
    130                 135                 140                 


Ile Asn Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr 
145                 150                 155                 160 


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Val Leu Ile 
                165                 170                 175     


Tyr Asp Ala Ser Asn Leu Val Ser Gly Val Pro Asp Arg Phe Ser Gly 
            180                 185                 190         


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 
        195                 200                 205             


Ala Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Thr Glu Asp Pro Trp 
    210                 215                 220                 


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
225                 230                 235     


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region (VH) complementarity determining 
       region (CDR) CDR1

<400>  15

Asn Tyr Trp Ile Asn 
1               5   


<210>  16
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region (VH) CDR2

<400>  16

Asn Ile Tyr Pro Ser Asp Ser Phe Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Asp 
    


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region (VH) CDR3

<400>  17

Asp Thr Gln Glu Arg Ser Trp Tyr Phe Asp Val 
1               5                   10      


<210>  18
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region (VL) CDR1

<400>  18

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region (VL) CDR2

<400>  19

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region (VL) CDR3

<400>  20

Ser Gln Ser Thr His Val Pro Trp Thr 
1               5                   


<210>  21
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Murine CD22ALAb VH sequence

<400>  21

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Tyr Pro Ser Asp Ser Phe Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Pro Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Asp Thr Gln Glu Arg Ser Trp Tyr Phe Asp Val Trp Gly Ala 
            100                 105                 110         


Gly Thr Thr Val Thr Val Ser Ser 
        115                 120 


<210>  22
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Humanised CD22ALAb VH sequence

<400>  22

Glu Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Tyr Pro Ser Asp Ser Phe Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Arg Asn Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Asp Thr Gln Glu Arg Ser Trp Tyr Phe Asp Val Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  23
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Murine CD22ALAb VL sequence

<400>  23

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Leu Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  24
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Humanised CD22ALAb VL sequence

<400>  24

Asp Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Val Glu Phe Thr Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Leu Gln Ser Glu Asp Phe Ala Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Trp Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


<210>  25
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Murine CD22ALAb scFv sequence

<400>  25

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Tyr Pro Ser Asp Ser Phe Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Pro Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Asp Thr Gln Glu Arg Ser Trp Tyr Phe Asp Val Trp Gly Ala 
            100                 105                 110         


Gly Thr Thr Val Thr Val Ser Ser Asp Val Val Met Thr Gln Thr Pro 
        115                 120                 125             


Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg 
    130                 135                 140                 


Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp 
145                 150                 155                 160 


Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val 
                165                 170                 175     


Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
            180                 185                 190         


Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu 
        195                 200                 205             


Gly Leu Tyr Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly 
    210                 215                 220                 


Gly Gly Thr Lys Leu Glu Ile Lys 
225                 230         


<210>  26
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR, Humanised CD22ALAb scFv sequence

<400>  26

Glu Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Tyr Pro Ser Asp Ser Phe Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Glu Leu Arg Asn Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Asp Thr Gln Glu Arg Ser Trp Tyr Phe Asp Val Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Asp Ile Val Met Thr Gln Ser Pro 
        115                 120                 125             


Ala Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg 
    130                 135                 140                 


Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His Trp 
145                 150                 155                 160 


Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Lys Val 
                165                 170                 175     


Ser Asn Arg Phe Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser 
            180                 185                 190         


Gly Val Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe 
        195                 200                 205             


Ala Val Tyr Tyr Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly 
    210                 215                 220                 


Gln Gly Thr Arg Leu Glu Ile Lys 
225                 230         


<210>  27
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TetR binding domain

<400>  27

Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      


Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              


Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  


Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  


Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
                85                  90                  95      


Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His 
    130                 135                 


<210>  28
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TiP binding domain

<400>  28

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly 
1               5                   10                  15      


Ser 
    


<210>  29
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified CD4 endodomain linker

<400>  29

Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly 
1               5                   10                  15      


Leu Gly Ile Phe Phe Cys Val Arg Cys Arg His Arg Arg Arg Gln Ala 
            20                  25                  30          


Glu Arg Met Ala Gln Ile Lys Arg Val Val Ser Glu Lys Lys Thr Ala 
        35                  40                  45              


Gln Ala Pro His Arg Phe Gln Lys Thr Cys Ser Pro Ile 
    50                  55                  60      


<210>  30
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, long nanotag

<400>  30

Asp Val Glu Ala Trp Leu Asp Glu Arg Val Pro Leu Val Glu Thr 
1               5                   10                  15  


<210>  31
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, short nanotag

<400>  31

Asp Val Glu Ala Trp Leu Gly Ala Arg 
1               5                   


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, streptag

<400>  32

Trp Arg His Pro Gln Phe Gly Gly 
1               5               


<210>  33
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, streptagII

<400>  33

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  34
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, SBP-tag

<400>  34

Met Asp Glu Lys Thr Thr Gly Trp Arg Gly Gly His Val Val Glu Gly 
1               5                   10                  15      


Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg Leu Glu His His Pro 
            20                  25                  30          


Gln Gly Gln Arg Glu Pro 
        35              


<210>  35
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, ccstreptag

<400>  35

Cys His Pro Gln Gly Pro Pro Cys 
1               5               


<210>  36
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Biotin mimicking peptide, flankedccstreptag

<400>  36

Ala Glu Cys His Pro Gln Gly Pro Pro Cys Ile Glu Gly Arg Lys 
1               5                   10                  15  


<210>  37
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  core streptavidin sequence

<400>  37

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser 
            20                  25                  30          


Ala Val Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  38
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, hinge-CH2CH3 of human IgG1

<400>  38

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp 
225                 230                 


<210>  39
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human CD8 stalk

<400>  39

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45      


<210>  40
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human IgG1 hinge

<400>  40

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Lys Asp Pro Lys 
            20  


<210>  41
<211>  185
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, CD2 ectodomain

<400>  41

Lys Glu Ile Thr Asn Ala Leu Glu Thr Trp Gly Ala Leu Gly Gln Asp 
1               5                   10                  15      


Ile Asn Leu Asp Ile Pro Ser Phe Gln Met Ser Asp Asp Ile Asp Asp 
            20                  25                  30          


Ile Lys Trp Glu Lys Thr Ser Asp Lys Lys Lys Ile Ala Gln Phe Arg 
        35                  40                  45              


Lys Glu Lys Glu Thr Phe Lys Glu Lys Asp Thr Tyr Lys Leu Phe Lys 
    50                  55                  60                  


Asn Gly Thr Leu Lys Ile Lys His Leu Lys Thr Asp Asp Gln Asp Ile 
65                  70                  75                  80  


Tyr Lys Val Ser Ile Tyr Asp Thr Lys Gly Lys Asn Val Leu Glu Lys 
                85                  90                  95      


Ile Phe Asp Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser 
            100                 105                 110         


Trp Thr Cys Ile Asn Thr Thr Leu Thr Cys Glu Val Met Asn Gly Thr 
        115                 120                 125             


Asp Pro Glu Leu Asn Leu Tyr Gln Asp Gly Lys His Leu Lys Leu Ser 
    130                 135                 140                 


Gln Arg Val Ile Thr His Lys Trp Thr Thr Ser Leu Ser Ala Lys Phe 
145                 150                 155                 160 


Lys Cys Thr Ala Gly Asn Lys Val Ser Lys Glu Ser Ser Val Glu Pro 
                165                 170                 175     


Val Ser Cys Pro Glu Lys Gly Leu Asp 
            180                 185 


<210>  42
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, CD34 ectodomain

<400>  42

Ser Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro Thr Gln Gly 
1               5                   10                  15      


Thr Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr Thr Thr 
            20                  25                  30          


Pro Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser Gln His Gly 
        35                  40                  45              


Asn Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys Phe Thr Ser 
    50                  55                  60                  


Thr Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser Ser Val Gln 
65                  70                  75                  80  


Ser Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro Ala Asn Val 
                85                  90                  95      


Ser Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro Gly Asn Val 
            100                 105                 110         


Ser Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser Pro Thr Lys 
        115                 120                 125             


Pro Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys Ala Glu Ile 
    130                 135                 140                 


Lys Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly Ile Cys Leu 
145                 150                 155                 160 


Glu Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys Asp Arg Gly 
                165                 170                 175     


Glu Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala Asp Ala Asp 
            180                 185                 190         


Ala Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser Glu Val Arg 
        195                 200                 205             


Pro Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu Ile Ser Ser 
    210                 215                 220                 


Lys Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys Lys Leu Gly 
225                 230                 235                 240 


Ile Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln Ser Tyr Ser 
                245                 250                 255     


Gln Lys Thr 
            


<210>  43
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  tyrp-1 transmembrane sequence

<400>  43

Ile Ile Ala Ile Ala Val Val Gly Ala Leu Leu Leu Val Ala Leu Ile 
1               5                   10                  15      


Phe Gly Thr Ala Ser Tyr Leu Ile 
            20                  


<210>  44
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 Z endodomain

<400>  44

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  45
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain and CD3 Z endodomain

<400>  45

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe 
            20                  25                  30          


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
        35                  40                  45              


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
    50                  55                  60                  


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
65                  70                  75                  80  


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
                85                  90                  95      


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
            100                 105                 110         


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
        115                 120                 125             


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    130                 135                 140 


<210>  46
<211>  180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain and CD28 and CD3 Zeta endodomains

<400>  46

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
65                  70                  75                  80  


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
                85                  90                  95      


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
            100                 105                 110         


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        115                 120                 125             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    130                 135                 140                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
145                 150                 155                 160 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                165                 170                 175     


Leu Pro Pro Arg 
            180 


<210>  47
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain and CD28, OX40 and CD3 Zeta endodomains

<400>  47

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro 
65                  70                  75                  80  


Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp 
                85                  90                  95      


Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser Arg Ser Ala 
            100                 105                 110         


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
        115                 120                 125             


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
    130                 135                 140                 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
145                 150                 155                 160 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                165                 170                 175     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            180                 185                 190         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        195                 200                 205             


His Met Gln Ala Leu Pro Pro Arg 
    210                 215     


<210>  48
<211>  20
<212>  PRT
<213>  Thosea asigna virus

<400>  48

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  49
<211>  1133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19/CD22 'OR' gate construct

<400>  49

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Leu 
            20                  25                  30          


Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
        35                  40                  45              


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
    50                  55                  60                  


Tyr Thr Phe Thr Ser Asn Trp Met His Trp Val Arg Gln Ala Pro Gly 
65                  70                  75                  80  


Gln Gly Leu Glu Trp Met Gly Glu Ile Asp Pro Ser Asp Ser Tyr Thr 
                85                  90                  95      


Asn Tyr Asn Gln Lys Phe Lys Gly Arg Val Thr Ile Thr Val Asp Lys 
            100                 105                 110         


Ser Ala Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
        115                 120                 125             


Thr Ala Val Tyr Tyr Cys Ala Arg Gly Ser Asn Pro Tyr Tyr Tyr Ala 
    130                 135                 140                 


Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val 
                165                 170                 175     


Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala 
            180                 185                 190         


Thr Leu Ser Cys Ser Ala Ser Ser Gly Val Asn Tyr Met His Trp Tyr 
        195                 200                 205             


Gln Gln Lys Pro Gly Gln Ala Pro Arg Arg Trp Ile Tyr Asp Thr Ser 
    210                 215                 220                 


Lys Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly 
225                 230                 235                 240 


Thr Ser Tyr Ser Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala 
                245                 250                 255     


Val Tyr Tyr Cys His Gln Arg Gly Ser Tyr Thr Phe Gly Gly Gly Thr 
            260                 265                 270         


Lys Leu Glu Ile Lys Arg Ser Asp Pro Thr Thr Thr Pro Ala Pro Arg 
        275                 280                 285             


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
    290                 295                 300                 


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
305                 310                 315                 320 


Leu Asp Phe Ala Cys Asp Ile Phe Trp Val Leu Val Val Val Gly Gly 
                325                 330                 335     


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
            340                 345                 350         


Trp Val Arg Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
        355                 360                 365             


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
    370                 375                 380                 


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
385                 390                 395                 400 


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
                405                 410                 415     


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
            420                 425                 430         


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
        435                 440                 445             


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
    450                 455                 460                 


Pro Pro Arg Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
465                 470                 475                 480 


Val Glu Glu Asn Pro Gly Pro Met Glu Phe Gly Leu Ser Trp Leu Phe 
                485                 490                 495     


Leu Val Ala Ile Leu Lys Gly Val Gln Cys Glu Val Gln Leu Val Glu 
            500                 505                 510         


Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
        515                 520                 525             


Ala Ala Ser Gly Phe Ala Phe Ser Ile Tyr Asp Met Ser Trp Val Arg 
    530                 535                 540                 


Gln Val Pro Gly Lys Gly Leu Glu Trp Val Ser Tyr Ile Ser Ser Gly 
545                 550                 555                 560 


Gly Gly Thr Thr Tyr Tyr Pro Asp Thr Val Lys Gly Arg Phe Thr Ile 
                565                 570                 575     


Ser Arg Asp Asn Ser Arg Asn Thr Leu Asp Leu Gln Met Asn Ser Leu 
            580                 585                 590         


Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg His Ser Gly Tyr 
        595                 600                 605             


Gly Ser Ser Tyr Gly Val Leu Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
    610                 615                 620                 


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
625                 630                 635                 640 


Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
                645                 650                 655     


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
            660                 665                 670         


Ile Ser Asn Tyr Leu Asn Trp Leu Gln Gln Lys Pro Gly Lys Ala Pro 
        675                 680                 685             


Lys Leu Leu Ile Tyr Tyr Thr Ser Ile Leu His Ser Gly Val Pro Ser 
    690                 695                 700                 


Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser 
705                 710                 715                 720 


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Asn 
                725                 730                 735     


Thr Leu Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
            740                 745                 750         


Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro 
        755                 760                 765             


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
    770                 775                 780                 


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
785                 790                 795                 800 


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
                805                 810                 815     


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
            820                 825                 830         


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
        835                 840                 845             


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
    850                 855                 860                 


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
865                 870                 875                 880 


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
                885                 890                 895     


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
            900                 905                 910         


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
        915                 920                 925             


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
    930                 935                 940                 


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
945                 950                 955                 960 


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
                965                 970                 975     


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys 
            980                 985                 990         


Phe Trp Val Leu Val Val Val Gly  Gly Val Leu Ala Cys  Tyr Ser Leu 
        995                 1000                 1005             


Leu Val  Thr Val Ala Phe Ile  Ile Phe Trp Val Arg  Ser Arg Val 
    1010                 1015                 1020             


Lys Phe  Ser Arg Ser Ala Asp  Ala Pro Ala Tyr Gln  Gln Gly Gln 
    1025                 1030                 1035             


Asn Gln  Leu Tyr Asn Glu Leu  Asn Leu Gly Arg Arg  Glu Glu Tyr 
    1040                 1045                 1050             


Asp Val  Leu Asp Lys Arg Arg  Gly Arg Asp Pro Glu  Met Gly Gly 
    1055                 1060                 1065             


Lys Pro  Arg Arg Lys Asn Pro  Gln Glu Gly Leu Tyr  Asn Glu Leu 
    1070                 1075                 1080             


Gln Lys  Asp Lys Met Ala Glu  Ala Tyr Ser Glu Ile  Gly Met Lys 
    1085                 1090                 1095             


Gly Glu  Arg Arg Arg Gly Lys  Gly His Asp Gly Leu  Tyr Gln Gly 
    1100                 1105                 1110             


Leu Ser  Thr Ala Thr Lys Asp  Thr Tyr Asp Ala Leu  His Met Gln 
    1115                 1120                 1125             


Ala Leu  Pro Pro Arg 
    1130             


<210>  50
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Kinesin motor protein: parallel 
       homodimer)

<400>  50

Met His Ala Ala Leu Ser Thr Glu Val Val His Leu Arg Gln Arg Thr 
1               5                   10                  15      


Glu Glu Leu Leu Arg Cys Asn Glu Gln Gln Ala Ala Glu Leu Glu Thr 
            20                  25                  30          


Cys Lys Glu Gln Leu Phe Gln Ser Asn Met Glu Arg Lys Glu Leu His 
        35                  40                  45              


Asn Thr Val Met Asp Leu Arg Gly Asn 
    50                  55          


<210>  51
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Hepatitis D delta antigen: 
       parallel homodimer)

<400>  51

Gly Arg Glu Asp Ile Leu Glu Gln Trp Val Ser Gly Arg Lys Lys Leu 
1               5                   10                  15      


Glu Glu Leu Glu Arg Asp Leu Arg Lys Leu Lys Lys Lys Ile Lys Lys 
            20                  25                  30          


Leu Glu Glu Asp Asn Pro Trp Leu Gly Asn Ile Lys Gly Ile Ile Gly 
        35                  40                  45              


Lys Tyr 
    50  


<210>  52
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Archaeal box C/D sRNP core 
       protein: anti-parallel heterodimer)

<400>  52

Arg Tyr Val Val Ala Leu Val Lys Ala Leu Glu Glu Ile Asp Glu Ser 
1               5                   10                  15      


Ile Asn Met Leu Asn Glu Lys Leu Glu Asp Ile Arg Ala Val Lys Glu 
            20                  25                  30          


Ser Glu Ile Thr Glu Lys Phe Glu Lys Lys Ile Arg Glu Leu Arg Glu 
        35                  40                  45              


Leu Arg Arg Asp Val Glu Arg Glu Ile Glu Glu Val Met 
    50                  55                  60      


<210>  53
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Mannose-binding protein A: 
       parallel homotrimer)

<400>  53

Ala Ile Glu Val Lys Leu Ala Asn Met Glu Ala Glu Ile Asn Thr Leu 
1               5                   10                  15      


Lys Ser Lys Leu Glu Leu Thr Asn Lys Leu His Ala Phe Ser Met 
            20                  25                  30      


<210>  54
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Coiled-coil serine-rich protein 
       1: parallel homotrimer)

<400>  54

Glu Trp Glu Ala Leu Glu Lys Lys Leu Ala Ala Leu Glu Ser Lys Leu 
1               5                   10                  15      


Gln Ala Leu Glu Lys Lys Leu Glu Ala Leu Glu His Gly 
            20                  25                  


<210>  55
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Polypeptide release factor 2: 
       anti-parallel heterotrimer Chain A)

<400>  55

Ile Asn Pro Val Asn Asn Arg Ile Gln Asp Leu Thr Glu Arg Ser Asp 
1               5                   10                  15      


Val Leu Arg Gly Tyr Leu Asp Tyr 
            20                  


<210>  56
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Polypeptide release factor 2: 
       anti-parallel heterotrimer Chain B)

<400>  56

Val Val Asp Thr Leu Asp Gln Met Lys Gln Gly Leu Glu Asp Val Ser 
1               5                   10                  15      


Gly Leu Leu Glu Leu Ala Val Glu Ala Asp Asp Glu Glu Thr Phe Asn 
            20                  25                  30          


Glu Ala Val Ala Glu Leu Asp Ala Leu Glu Glu Lys Leu Ala Gln Leu 
        35                  40                  45              


Glu Phe Arg 
    50      


<210>  57
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (SNAP-25 and SNARE: parallel 
       heterotetramer Chain A)

<400>  57

Ile Glu Thr Arg His Ser Glu Ile Ile Lys Leu Glu Asn Ser Ile Arg 
1               5                   10                  15      


Glu Leu His Asp Met Phe Met Asp Met Ala Met Leu Val Glu Ser Gln 
            20                  25                  30          


Gly Glu Met Ile Asp Arg Ile Glu Tyr Asn Val Glu His Ala Val Asp 
        35                  40                  45              


Tyr Val Glu 
    50      


<210>  58
<211>  67
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (SNAP-25 and SNARE: parallel 
       heterotetramer Chain B)

<400>  58

Ala Leu Ser Glu Ile Glu Thr Arg His Ser Glu Ile Ile Lys Leu Glu 
1               5                   10                  15      


Asn Ser Ile Arg Glu Leu His Asp Met Phe Met Asp Met Ala Met Leu 
            20                  25                  30          


Val Glu Ser Gln Gly Glu Met Ile Asp Arg Ile Glu Tyr Asn Val Glu 
        35                  40                  45              


His Ala Val Asp Tyr Val Glu Arg Ala Val Ser Asp Thr Lys Lys Ala 
    50                  55                  60                  


Val Lys Tyr 
65          


<210>  59
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (SNAP-25 and SNARE: parallel 
       heterotetramer Chain C)

<400>  59

Glu Leu Glu Glu Met Gln Arg Arg Ala Asp Gln Leu Ala Asp Glu Ser 
1               5                   10                  15      


Leu Glu Ser Thr Arg Arg Met Leu Gln Leu Val Glu Glu Ser Lys Asp 
            20                  25                  30          


Ala Gly Ile Arg Thr Leu Val Met Leu Asp Glu Gln Gly Glu Gln Leu 
        35                  40                  45              


Glu Arg Ile Glu Glu Gly Met Asp Gln Ile Asn Lys Asp Met Lys Glu 
    50                  55                  60                  


Ala Glu Lys Asn Leu 
65                  


<210>  60
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (SNAP-25 and SNARE: parallel 
       heterotetramer Chain D)

<400>  60

Ile Glu Thr Arg His Ser Glu Ile Ile Lys Leu Glu Asn Ser Ile Arg 
1               5                   10                  15      


Glu Leu His Asp Met Phe Met Asp Met Ala Met Leu Val Glu Ser Gln 
            20                  25                  30          


Gly Glu Met Ile Asp Arg Ile Glu Tyr Asn Val Glu His Ala Val Asp 
        35                  40                  45              


Tyr Val Glu 
    50      


<210>  61
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Lac repressor: parallel 
       homotetramer)

<400>  61

Ser Pro Arg Ala Leu Ala Asp Ser Leu Met Gln Leu Ala Arg Gln Val 
1               5                   10                  15      


Ser Arg Leu Glu 
            20  


<210>  62
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of coiled coil domain (Apolipoprotein E: anti-parallel 
       heterotetramer)

<400>  62

Ser Gly Gln Arg Trp Glu Leu Ala Leu Gly Arg Phe Trp Asp Tyr Leu 
1               5                   10                  15      


Arg Trp Val Gln Thr Leu Ser Glu Gln Val Gln Glu Glu Leu Leu Ser 
            20                  25                  30          


Ser Gln Val Thr Gln Glu Leu Arg Ala Leu Met Asp Glu Thr Met Lys 
        35                  40                  45              


Glu Leu Lys Ala Tyr Lys Ser Glu Leu Glu Glu Gln Leu Thr Ala Arg 
    50                  55                  60                  


Leu Ser Lys Glu Leu Gln Ala Ala Gln Ala Arg Leu Gly Ala Asp Met 
65                  70                  75                  80  


Glu Asp Val Cys Gly Arg Leu Val Gln Tyr Arg Gly Glu Val Gln Ala 
                85                  90                  95      


Met Leu Gly Gln Ser Thr Glu Glu Leu Arg Val Arg Leu Ala Ser His 
            100                 105                 110         


Leu Arg Lys Leu Arg Lys Arg Leu Leu Arg Asp Ala Asp Asp Leu Gln 
        115                 120                 125             


Lys Arg Leu Ala Val Tyr Gln Ala 
    130                 135     


<210>  63
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  COMP coiled-coil domain

<400>  63

Asp Leu Gly Pro Gln Met Leu Arg Glu Leu Gln Glu Thr Asn Ala Ala 
1               5                   10                  15      


Leu Gln Asp Val Arg Glu Leu Leu Arg Gln Gln Val Arg Glu Ile Thr 
            20                  25                  30          


Phe Leu Lys Asn Thr Val Met Glu Cys Asp Ala Cys Gly 
        35                  40                  45  


<210>  64
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated COMP coiled-coil domain (5 C-terminal amino acids)

<400>  64

Cys Asp Ala Cys Gly 
1               5   


<210>  65
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated COMP coiled-coil domain (C-terminus)

<400>  65

Gln Gln Val Arg Glu Ile Thr Phe Leu Lys Asn Thr Val Met Glu Cys 
1               5                   10                  15      


Asp Ala Cys Gly 
            20  


<210>  66
<211>  1139
<212>  DNA
<213>  Homo sapiens

<400>  66
atcccgccga gcccaaatct cctgacaaaa ctcacacatg cccaccgtgc ccagcacctg       60

aactcctggg gggaccgtca gtcttcctct tccccccaaa acccaaggac accctcatga      120

tctcccggac ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg      180

tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg      240

aggagcagta caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact      300

ggctgaatgg caaggagtac aagtgcaagg tctccaacaa agccctccca gcccccatcg      360

agaaaaccat ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc      420

catcccggga tgagctgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct      480

atcccagcga catcgccgtg gagtgggaga gcaatgggca accggagaac aactacaaga      540

ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctacagcaag ctcaccgtgg      600

acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcac gaggctctgc      660

acaaccacta cacgcagaag agcctctccc tgtctccggg taaaaaagat cccaaatttt      720

gggtgctggt ggtggttggt ggagtcctgg cttgctatag cttgctagta acagtggcct      780

ttattatttt ctgggtgagg agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc      840

agcagggcca gaaccagctc tataacgagc tcaatctagg acgaagagag gagtacgatg      900

ttttggacaa gagacgtggc cgggaccctg agatgggggg aaagccgaga aggaagaacc      960

ctcaggaagg cctgtacaat gaactgcaga aagataagat ggcggaggcc tacagtgaga     1020

ttgggatgaa aggcgagcgc cggaggggca aggggcacga tggcctttac cagggtctca     1080

gtacagccac caaggacacc tacgacgccc ttcacatgca ggccctgcct cctcgcaga      1139


<210>  67
<211>  1139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-wobbled HCH2CH3-CD28tmZeta

<400>  67
atccagccga accaaagagc cccgataaga cccacacctg tcccccctgc ccagccccag       60

agctgctggg aggccccagc gtgtttctgt ttccacccaa gccaaaggat accctgatga      120

ttagtagaac acccgaagtg acctgtgtgg tggtggatgt gtctcacgag gaccccgagg      180

tgaaatttaa ttggtatgtt gatggtgttg aagtgcacaa cgccaaaacc aaacccagag      240

aggagcagta caattctacc tatagagtcg tgtctgtgct gacagtgctg catcaggatt      300

ggctgaacgg aaaagaatac aaatgtaaag tgagcaataa ggccctgccc gctccaattg      360

agaagacaat tagcaaggcc aagggccagc caagggagcc ccaggtgtat acactgccac      420

ccagtagaga cgaactgaca aagaatcagg tgtctctgac atgtctggtg aagggatttt      480

acccatctga tatcgccgtg gaatgggaat ctaacggcca gcccgagaat aactataaga      540

caaccccacc agtgctggat agcgatggca gcttttttct gtattctaag ctgacagtgg      600

ataagtcccg gtggcagcag ggaaatgtgt ttagctgtag tgtcatgcat gaggccctgc      660

acaatcacta tacccagaaa tctctgagtc tgagcccagg caagaaggac cccaagttct      720

gggtcctggt ggtggtggga ggcgtgctgg cctgttactc tctcctggtg accgtggcct      780

tcatcatctt ttgggtgcgc tcccgggtga agttttctcg ctctgccgat gccccagcct      840

atcagcaggg ccagaatcag ctgtacaatg aactgaacct gggcaggcgg gaggagtacg      900

acgtgctgga taagcggaga ggcagagacc ccgagatggg cggcaaacca cggcgcaaaa      960

atccccagga gggactctat aacgagctgc agaaggacaa aatggccgag gcctattccg     1020

agatcggcat gaagggagag agaagacgcg gaaagggcca cgacggcctg tatcagggat     1080

tgtccaccgc tacaaaagat acatatgatg ccctgcacat gcaggccctg ccacccaga      1139


<210>  68
<211>  258
<212>  PRT
<213>  Homo sapiens

<400>  68

Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp Asn Ala Val Leu 
1               5                   10                  15      


Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln Gln Leu Thr Trp 
            20                  25                  30          


Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu Ser Leu Gly Leu 
        35                  40                  45              


Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ile Trp Leu Phe Ile 
    50                  55                  60                  


Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu Cys Gln Pro Gly 
65                  70                  75                  80  


Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr Val Asn Val Glu 
                85                  90                  95      


Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp Leu Gly Gly Leu 
            100                 105                 110         


Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro Ser Ser Pro Ser 
        115                 120                 125             


Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala Lys Asp Arg Pro 
    130                 135                 140                 


Glu Ile Trp Glu Gly Glu Pro Pro Cys Leu Pro Pro Arg Asp Ser Leu 
145                 150                 155                 160 


Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro Gly Ser Thr Leu 
                165                 170                 175     


Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser Arg Gly Pro Leu 
            180                 185                 190         


Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser Leu Leu Ser Leu 
        195                 200                 205             


Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp Val Met Glu Thr 
    210                 215                 220                 


Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala Gly Lys Tyr Tyr 
225                 230                 235                 240 


Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu Glu Ile Thr Ala 
                245                 250                 255     


Arg Pro 
        


<210>  69
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence of TiP

<400>  69

Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly Ser 
1               5                   10                  15      


<210>  70
<211>  87
<212>  PRT
<213>  Homo sapiens

<400>  70

Leu Ser Asp Ser Gly Gln Val Leu Leu Glu Ser Asn Ile Lys Val Leu 
1               5                   10                  15      


Pro Thr Trp Ser Thr Pro Val Gln Pro Met Ala Leu Ile Val Leu Gly 
            20                  25                  30          


Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe Cys 
        35                  40                  45              


Val Arg Cys Arg His Arg Arg Arg Gln Ala Glu Arg Met Ser Gln Ile 
    50                  55                  60                  


Lys Arg Leu Leu Ser Glu Lys Lys Thr Cys Gln Cys Pro His Arg Phe 
65                  70                  75                  80  


Gln Lys Thr Cys Ser Pro Ile 
                85          


<210>  71
<211>  926
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  basic TetCAR sequence

<400>  71

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly 
1               5                   10                  15      


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
            20                  25                  30          


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
        35                  40                  45              


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
    50                  55                  60                  


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
65                  70                  75                  80  


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
                85                  90                  95      


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
            100                 105                 110         


Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly 
        115                 120                 125             


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala 
    130                 135                 140                 


Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr Asp Ala 
145                 150                 155                 160 


Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
                165                 170                 175     


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp Ile Tyr 
            180                 185                 190         


Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        195                 200                 205             


Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser Arg Phe 
    210                 215                 220                 


Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser Ser Leu 
225                 230                 235                 240 


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys Asn Tyr 
                245                 250                 255     


Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ser Gly 
            260                 265                 270         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        275                 280                 285             


Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
    290                 295                 300                 


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
305                 310                 315                 320 


Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro Gly Lys 
                325                 330                 335     


Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser Thr Tyr 
            340                 345                 350         


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
        355                 360                 365             


Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
    370                 375                 380                 


Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly Tyr Phe 
385                 390                 395                 400 


Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met Asp Pro 
                405                 410                 415     


Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
            420                 425                 430         


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
        435                 440                 445             


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
    450                 455                 460                 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
465                 470                 475                 480 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
                485                 490                 495     


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
            500                 505                 510         


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
        515                 520                 525             


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
    530                 535                 540                 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
545                 550                 555                 560 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
                565                 570                 575     


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
            580                 585                 590         


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
        595                 600                 605             


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
    610                 615                 620                 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
625                 630                 635                 640 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Met Ala Leu Ile Val 
                645                 650                 655     


Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe 
            660                 665                 670         


Phe Cys Val Arg Cys Arg His Arg Arg Arg Gln Ala Glu Arg Met Ala 
        675                 680                 685             


Gln Ile Lys Arg Val Val Ser Glu Lys Lys Thr Ala Gln Ala Pro His 
    690                 695                 700                 


Arg Phe Gln Lys Thr Cys Ser Pro Ile Ser Gly Gly Gly Gly Ser Met 
705                 710                 715                 720 


Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu Leu 
                725                 730                 735     


Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln Lys 
            740                 745                 750         


Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys Arg 
        755                 760                 765             


Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His Thr 
    770                 775                 780                 


His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg Asn 
785                 790                 795                 800 


Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly Ala 
                805                 810                 815     


Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr Leu 
            820                 825                 830         


Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu Asn 
        835                 840                 845             


Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys Val 
    850                 855                 860                 


Leu Glu Asp Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu Thr Pro 
865                 870                 875                 880 


Thr Thr Asp Ser Met Pro Pro Leu Leu Arg Gln Ala Ile Glu Leu Phe 
                885                 890                 895     


Asp His Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu Ile 
            900                 905                 910         


Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Ser 
        915                 920                 925     


