               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> Recombinant yeast cell

<130> 32114-WO-PCT

<160> 119

<170> BiSSAP 1.3.6

<210> 1
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5793 - pUDR240 construction

<400> 1
gatcatttat ctttcactgc ggag                                           24


<210> 2
<211> 104
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6965 - pUDR240 construction

<400> 2
gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cgggcaagga     60

cgtcgaccat agttttagag ctagaaatag caagttaaaa taag                     104


<210> 3
<211> 104
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6966 - pUDR240 construction

<400> 3
gtgcgcatgt ttcggcgttc gaaacttctc cgcagtgaaa gataaatgat cccaagaatt     60

cccattattc ggttttagag ctagaaatag caagttaaaa taag                     104


<210> 4
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5792 pUDR119 and pUDR164 construction

<400> 4
gttttagagc tagaaatagc aagttaaaat aag                                  33


<210> 5
<211> 17
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5980 - pUDR119 and pUDR164 construction

<400> 5
cgaccgagtt gctcttg                                                   17


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5979 - pUDR119 and pUDR164 construction

<400> 6
tattgacgcc gggcaagagc                                                20


<210> 7
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7023 - pUDR119 construction

<400> 7
gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac gaagaattcc     60

agtggtcaat gatcatttat ctttcactgc ggagaagttt cgaacgccga aacatgcgca    120


<210> 8
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7374 - pUDR164 construction

<400> 8
gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac ctactctctt     60

cctagtcgcc gatcatttat ctttcactgc ggagaagttt cgaacgccga aacatgcgca    120


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7375 - pUDR164 diagnostic PCR

<400> 9
ctactctctt cctagtcgcc                                                20


<210> 10
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7549 - Addition of 20 bp primer-binding sequence to cbbM

<400> 10
gcgataccct gcgatcttca cagctatgac catgattacg                           40


<210> 11
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7550 - Addition of 20 bp primer-binding sequence to cbbM

<400> 11
cgcgcagatt agcgaagcct cactataggg cgaattgg                             38


<210> 12
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7074 - cbbM cassette construction - D tag addition (single copy
      cbbm-prk-chaperone integration)

<400> 12
acgcatctac gactgtgggt cccgtggaga aatgtatgaa accctgtatg gagagtgatt     60

gctggagctc agtttatcat t                                               81


<210> 13
<211> 80
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7075 - cbbM cassette construction - J tag addition (single copy
      cbbm-prk-chaperone integration)

<400> 13
cgacgagatg ctcagactat gtgttctacc tgcttggaca tcttcgcgta tatgacggcc     60

cggccgcaaa ttaaagcctt                                                 80


<210> 14
<211> 88
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7548 - cbbM cassette construction - SGA1 tag addition

<400> 14
gcatagaaca ttatccgcgg aaacgggtat taggggtgag ggtgaataag gaaagtcagg     60

gaaatcgggc cgcgcagatt agcgaagc                                        88


<210> 15
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6285 - cbbM cassette construction - G tag addition

<400> 15
aagggccatg accacctgat gcaccaatta ggtaggtctg gctatgtcta tacctctggc     60

gcgataccct gcgatcttc                                                  79


<210> 16
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6280 - cbbM cassette construction - A tag addition

<400> 16
gtgcctattg atgatctggc ggaatgtctg ccgtgccata gccatgcctt cacatatagt     60

gcgataccct gcgatcttc                                                  79


<210> 17
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6273 - cbbM cassette construction - G tag addition

<400> 17
gccagaggta tagacatagc cagacctacc taattggtgc atcaggtggt catggccctt     60

cgcgcagatt agcgaagc                                                   78


<210> 18
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6281 - cbbM cassette construction - B tag addition

<400> 18
gttgaacatt cttaggctgg tcgaatcatt tagacacggg catcgtcctc tcgaaaggtg     60

gcgataccct gcgatcttc                                                  79


<210> 19
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6270 - cbbM cassette construction - A tag addition

<400> 19
actatatgtg aaggcatggc tatggcacgg cagacattcc gccagatcat caataggcac     60

cgcgcagatt agcgaagc                                                   78


<210> 20
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6282 - cbbM cassette construction - C tag addition

<400> 20
ctagcgtgtc ctcgcatagt tcttagattg tcgctacggc atatacgatc cgtgagacgt     60

gcgataccct gcgatcttc                                                  79


<210> 21
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6271 - cbbM cassette construction - B tag addition

<400> 21
cacctttcga gaggacgatg cccgtgtcta aatgattcga ccagcctaag aatgttcaac     60

cgcgcagatt agcgaagc                                                   78


<210> 22
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6284 - cbbM cassette construction - D tag addition

<400> 22
aatcactctc catacagggt ttcatacatt tctccacggg acccacagtc gtagatgcgt     60

gcgataccct gcgatcttc                                                  79


<210> 23
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6272 - cbbM cassette construction - C tag addition

<400> 23
acgtctcacg gatcgtatat gccgtagcga caatctaaga actatgcgag gacacgctag     60

cgcgcagatt agcgaagc                                                   78


<210> 24
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6283 cbbM cassette construction - D tag addition

<400> 24
acgcatctac gactgtgggt cccgtggaga aatgtatgaa accctgtatg gagagtgatt     60

gcgataccct gcgatcttc                                                  79


<210> 25
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6275 - cbbM cassette construction - M tag addition

<400> 25
acgagagatg aaggctcacc gatggactta gtatgatgcc atgctggaag ctccggtcat     60

cgcgcagatt agcgaagc                                                   78


<210> 26
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6287 - cbbM cassette construction - M tag addition

<400> 26
atgaccggag cttccagcat ggcatcatac taagtccatc ggtgagcctt catctctcgt     60

gcgataccct gcgatcttc                                                  79


<210> 27
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6276 - cbbM cassette construction - N tag addition

<400> 27
ttctaggctt tgatgcaagg tccacatatc ttcgttagga ctcaatcgtg gctgctgatc     60

cgcgcagatt agcgaagc                                                   78


<210> 28
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6288 - cbbM cassette construction - N tag addition

<400> 28
gatcagcagc cacgattgag tcctaacgaa gatatgtgga ccttgcatca aagcctagaa     60

gcgataccct gcgatcttc                                                  79


<210> 29
<211> 78
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6277 - cbbM cassette construction - O tag addition

<400> 29
atactccctg cacagatgag tcaagctatt gaacaccgag aacgcgctga acgatcattc     60

cgcgcagatt agcgaagc                                                   78


<210> 30
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6289 - cbbM cassette construction - O tag addition

<400> 30
gaatgatcgt tcagcgcgtt ctcggtgttc aatagcttga ctcatctgtg cagggagtat     60

gcgataccct gcgatcttc                                                  79


<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7298 - Diagnostic primer cbbM integration

<400> 31
ttgttcaatg gatgcggttc                                                20


<210> 32
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4692 - Diagnostic primer cbbM integration

<400> 32
aagggccatg accacctg                                                  18


<210> 33
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4870 - Diagnostic primer cbbM integration

<400> 33
gccagaggta tagacatagc c                                              21


<210> 34
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3275 - Diagnostic primer cbbM integration

<400> 34
gtgcctattg atgatctggc ggaatg                                         26


<210> 35
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3847 - Diagnostic primer cbbM integration

<400> 35
actatatgtg aaggcatggc tatgg                                          25


<210> 36
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3276 - Diagnostic primer cbbM integration

<400> 36
gttgaacatt cttaggctgg tcgaatc                                        27


<210> 37
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4691 - Diagnostic primer cbbM integration

<400> 37
cacctttcga gaggacgatg                                                20


<210> 38
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3277 - Diagnostic primer cbbM integration

<400> 38
ctagcgtgtc ctcgcatagt tcttagattg                                     30


<210> 39
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3283 - Diagnostic primer cbbM integration

<400> 39
acgtctcacg gatcgtatat gc                                             22


<210> 40
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7226 - Diagnostic primer cbbM integration

<400> 40
atacatttct ccacgggacc                                                20


<210> 41
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7225 - Diagnostic primer cbbM integration

<400> 41
gtcccgtgga gaaatgtatg                                                20


<210> 42
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6629 - Diagnostic primer cbbM integration

<400> 42
acgagagatg aaggctcacc                                                20


<210> 43
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4184 - Diagnostic primer cbbM integration

<400> 43
atgaccggag cttccagcat g                                              21


<210> 44
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3842 - Diagnostic primer cbbM integration

<400> 44
ttctaggctt tgatgcaagg tc                                             22


<210> 45
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3843	Diagnostic primer cbbM integration

<400> 45
gatcagcagc cacgattg                                                  18


<210> 46
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3840 - Diagnostic primer cbbM integration

<400> 46
atactccctg cacagatga                                                 19


<210> 47
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3837 Diagnostic primer cbbM integration

<400> 47
gaatgatcgt tcagcgcg                                                  18


<210> 48
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7228 - Diagnostic primer cbbM integration

<400> 48
tctacctgct tggacatctt c                                              21


<210> 49
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7227 - Diagnostic primer cbbM integration

<400> 49
atacgcgaag atgtccaagc                                                20


<210> 50
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3290 - Diagnostic primer cbbM integration

<400> 50
gtcacgggtt ctcagcaatt cg                                             22


<210> 51
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3291 - Diagnostic primer cbbM integration

<400> 51
ctctaacgcc tcagccatca tcg                                            23


<210> 52
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4229 - Diagnostic primer cbbM integration

<400> 52
tggtcgacag atacaatcct gg                                             22


<210> 53
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 1370 - Diagnostic primer cbbM integration

<400> 53
gtttgatggt ccagctacag                                                20


<210> 54
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 1371 - Diagnostic primer cbbM integration

<400> 54
aaatagcacc agcagcagga g                                              21


<210> 55
<211> 83
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7076 - groEL cassette construction - J tag addition

<400> 55
ggccgtcata tacgcgaaga tgtccaagca ggtagaacac atagtctgag catctcgtcg     60

cgaccatagc ttcaaaatgt ttc                                             83


<210> 56
<211> 83
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7077 - groEL cassette construction - H tag addition

<400> 56
gtcacgggtt ctcagcaatt cgagctatta ccgatgatgg ctgaggcgtt agagtaatct     60

gctcgacatt ttatgatgga atg                                             83


<210> 57
<211> 82
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7078 - groES cassette construction - H tag addition

<400> 57
agattactct aacgcctcag ccatcatcgg taatagctcg aattgctgag aacccgtgac     60

gggatctacg tatggtcatt tc                                              82


<210> 58
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7079 - groES cassette construction - SGA1 tag addition

<400> 58
tatatttgat gtaaatatct aggaaataca cttgtgtata cttctcgctt ttcttttatt     60

cgccctttta aacagttgat g                                               81


<210> 59
<211> 79
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7082 - LYS1p prk cassette construction

<400> 59
acgtctcacg gatcgtatat gccgtagcga caatctaaga actatgcgag gacacgctag     60

aacgctggtg tcctagagc                                                  79


<210> 60
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7083 - LYS1p prk cassette construction

<400> 60
ttttgcggtt gtgtgaaaaa t                                              21


<210> 61
<211> 80
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7292	UBC6p prk cassette construction

<400> 61
acgtctcacg gatcgtatat gccgtagcga caatctaaga actatgcgag gacacgctag     60

gcccgcgatt tatccttctt                                                 80


<210> 62
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7294 - UBC6p prk cassette construction

<400> 62
tactattgta cgtactttgt ttgca                                          25


<210> 63
<211> 82
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7293 - YEN1p prk cassette construction

<400> 63
acgtctcacg gatcgtatat gccgtagcga caatctaaga actatgcgag gacacgctag     60

ggaattcaag caatagcgtt tc                                              82


<210> 64
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7295 - YEN1p prk cassette construction

<400> 64
tttcttgtgc agtatccaga atat                                           24


<210> 65
<211> 80
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7930 - DAN1p prk cassette construction

<400> 65
tcacagaggg atcccgttac ccatctatgc tgaagattta tcatactatt cctccgctcg     60

agaatgcaag ggcaaacagg                                                 80


<210> 66
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7931 - DAN1p prk cassette construction

<400> 66
tacttggggt atatatttag tatgc                                          25


<210> 67
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7084 - prk cassette construction (PGK1t)

<400> 67
attgaattga attgaaatcg atag                                           24


<210> 68
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7085 - prk cassette construction (PGK1t) - D tag addition (single
      copy cbbm-prk-chaperone integration)

<400> 68
aatcactctc catacagggt ttcatacatt tctccacggg acccacagtc gtagatgcgt     60

gcttcaagct tacacaacac g                                               81


<210> 69
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7934 - prk cassette construction (PGK1t) - X-2 tag addition

<400> 69
gtcataactc aatttgccta tttcttacgg cttctcataa aacgtcccac actattcagg     60

gcttcaagct tacacaacac g                                               81


<210> 70
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7080 - prk amplification (LYS1p cassette)

<400> 70
aaacgcttta tttttcacac aaccgcaaaa atgtcacaac aacaaacaat tgtg           54


<210> 71
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7081 - prk amplification

<400> 71
attgatctat cgatttcaat tcaattcaat ctaggcttta gcagctgttg                50


<210> 72
<211> 56
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7296 - prk amplification (UBC6p cassette)

<400> 72
cgcaaattgc aaacaaagta cgtacaatag taatgtcaca acaacaaaca attgtg         56


<210> 73
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7297 - prk amplification (YEN1p cassette)

<400> 73
acttgtatat tctggatact gcacaagaaa atgtcacaac aacaaacaat tgtg           54


<210> 74
<211> 81
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7932 - prk amplification (DAN1p cassette)

<400> 74
ttgcagataa aagtgtagca gataaaagtg tagcatacta aatatatacc ccaagtaatg     60

tcacaacaac aaacaattgt g                                               81


<210> 75
<211> 82
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7086 - URA3 amplification - SGA1 tag addition (single copy
      cbbm-prk-chaperone integration)

<400> 75
tttacaatat agtgataatc gtggactaga gcaagatttc aaataagtaa cagcagcaaa     60

gatcccaata caacagatca cg                                              82


<210> 76
<211> 83
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7087 - URA3 amplification - C tag addition (single copy
      cbbm-prk-chaperone integration)

<400> 76
ctagcgtgtc ctcgcatagt tcttagattg tcgctacggc atatacgatc cgtgagacgt     60

ctcgagagct cgttttattt agg                                             83


<210> 77
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4221 - Diagnostic primer single copy cbbm-prk-chaperone
      integration

<400> 77
aaacttagat tagattgcta tgctttc                                        27


<210> 78
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 2674 - Diagnostic primer single copy cbbm-prk-chaperone
      integration

<400> 78
atagccaccc aaggcatttc                                                20


<210> 79
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7376 - Diagnostic primer prk integration in X-2

<400> 79
ggtctaggcc tgcataatcg                                                20


<210> 80
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 7377 - Diagnostic primer prk integration in X-2

<400> 80
tgcggcatca tgtctacttg                                                20


<210> 81
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> 2375 - Diagnostic primer prk integration in X-2

<400> 81
tgagccactt aaatttcgtg aatg                                           24


<210> 82
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 2376 - Diagnostic primer prk integration in X-2

<400> 82
gcctttgagt gagctgatac c                                              21


<210> 83
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6967 - Repair fragment GPD1

<400> 83
tggtattggc agtttcgtag ttatatatat actaccatga gtgaaactgt tacgttacct     60

gcattatgtc atttctcata actactttat cacgttagaa attacttatt attattaaat    120


<210> 84
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6968 - Repair fragment GPD1

<400> 84
atttaataat aataagtaat ttctaacgtg ataaagtagt tatgagaaat gacataatgc     60

aggtaacgta acagtttcac tcatggtagt atatatataa ctacgaaact gccaatacca    120


<210> 85
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6969 - Repair fragment GPD2

<400> 85
gtattttggt agattcaatt ctctttccct ttccttttcc ttcgctcccc ttccttatca     60

aaccaattta tcattataca caagttctac aactactact agtaacatta ctacagttat    120


<210> 86
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> 6970 - Repair fragment GPD2

<400> 86
ataactgtag taatgttact agtagtagtt gtagaacttg tgtataatga taaattggtt     60

tgataaggaa ggggagcgaa ggaaaaggaa agggaaagag aattgaatct accaaaatac    120


<210> 87
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4398 - Diagnostic primer GPD1 deletion

<400> 87
tctcacctct caccgctgac                                                20


<210> 88
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4401 - Diagnostic primer GPD1 deletion

<400> 88
acggtgagct ccgtattatc                                                20


<210> 89
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> 2112 - Diagnostic primer GPD2 deletion

<400> 89
acggacctat tgccattg                                                  18


<210> 90
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 2015 - Diagnostic primer GPD2 deletion

<400> 90
ccaaatgcga catgagtcac                                                20


<210> 91
<211> 600
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Promoter ANB1

<400> 91
agatcggtga aaacatcttg atcttgctcc cgggaatttt agattcaggt aggaaattga      60

ttacatcaat actgttaccc tgaatcatat tcgacgatgt cgtctcacac ggaaatataa     120

ttcatttctt ggttttccaa aaaaattttc attttttttc acttttttgt ttcgtcctcc     180

tttttttttt ttttttttta ttttttttcc tgtgttcacc tttttttttt tcagttgaca     240

tctttctgca ttcttttctg tgtttttttt tttttttttc gtttttccat tgttcgttcg     300

ttgcctgttt tttcgcccta ttgttctcga gcctaaaaat tttttccttt cctgctttcc     360

tttcttcgtt caaagtttcc tattccattg ttctctttgg taaactcatt gttgtcggaa     420

ctcagatata ttcaggtcaa tttactgtac ttcaattgac ttttttcttg aaatttcaac     480

ttgccttttc aacttgttct tcttttttaa tcttattcta cactttagtt cccttacctt     540

gttcctaatt attgtctagc aaaaagaaaa catacaccta tttcattcac acactaaaac     600


<210> 92
<211> 825
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Promoter DAN1

<400> 92
agaatgcaag ggcaaacagg cctgaggtaa ctttaaaata taatgtacct tgaaaactat      60

gtatacattg catttattaa gcagtagggt attttgtttg caagaaaata aagctcaaaa     120

tatctttgga gtttgacaat agtatagaaa aaatagctca gcaaccttaa taaaaaaagg     180

gtcattgggc aaagcgtttt aaaaagattt aagtggtagt gtttaaagtg aaagagattg     240

atatagttaa aaattgttga gctcaattca cgctggattc ggcgatccgt tttcttcaat     300

cctcacgtgc tttcttcgtt tgagtgcaaa agttcatatg atgctatctc ccgcttatct     360

tattagtcga aaatggggag aatttcctat tttatctgtc gtttagcaca tacggccagg     420

aaaatacata agatttcgcc gaacgacggg gtcaattcgt cctttttgta cacatcgttt     480

aatttatgag gaaaaattga tgaacgtatc ctccgtagac gctcctctga aaagtttcat     540

gtttcctgcg cgttcctttg ataggcaata aaacaataca acgcgtgcct ttgaaaatgc     600

caagatctat acgaggcctc taacaaaaca tcgttcagga acagagaatg ctaaaaatgc     660

aaaagggtcc ctgggtactc attgaataga aatgattgaa aatactgcgt ataaaatagc     720

acgactaaat gatactattt ttatgtcgac acggtactat ttcttctttt gcagataaaa     780

gtgtagcaga taaaagtgta gcatactaaa tatatacccc aagta                     825


<210> 93
<211> 1000
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_DAN1 promoter

<400> 93
gtttatggat gcacttaggg gtcctacaag ccttcgtatg aagttgcccc tcgcaagcac      60

actataaatt catgaacaat tgcgcagtca aagttagatt gtaagccgaa ttcaaacgat     120

ttcaacagtc gtagctaacg aggccttttg tctcacaccc ttacaaaggg actgtagaat     180

gcaagggcaa acaggcctga ggtaacttta aaatataatg taccttgaaa actatgtata     240

cattgcattt attaagcagt agggtatttt gtttgcaaga aaataaagct caaaatatct     300

ttggagtttg acaatagtat agaaaaaata gctcagcaac cttaataaaa aaagggtcat     360

tgggcaaagc gttttaaaaa gatttaagtg gtagtgttta aagtgaaaga gattgatata     420

gttaaaaatt gttgagctca attcacgctg gattcggcga tccgttttct tcaatcctca     480

cgtgctttct tcgtttgagt gcaaaagttc atatgatgct atctcccgct tatcttatta     540

gtcgaaaatg gggagaattt cctattttat ctgtcgttta gcacatacgg ccaggaaaat     600

acataagatt tcgccgaacg acggggtcaa ttcgtccttt ttgtacacat cgtttaattt     660

atgaggaaaa attgatgaac gtatcctccg tagacgctcc tctgaaaagt ttcatgtttc     720

ctgcgcgttc ctttgatagg caataaaaca atacaacgcg tgcctttgaa aatgccaaga     780

tctatacgag gcctctaaca aaacatcgtt caggaacaga gaatgctaaa aatgcaaaag     840

ggtccctggg tactcattga atagaaatga ttgaaaatac tgcgtataaa atagcacgac     900

taaatgatac tatttttatg tcgacacggt actatttctt cttttgcaga taaaagtgta     960

gcagataaaa gtgtagcata ctaaatatat accccaagta                          1000


<210> 94
<211> 967
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_DIP5 promoter

<400> 94
gctctcttat caattatgta agtgcttgta tactatttac ctaagataag aaaaaaaaaa      60

aagcaattca aaattaagct tatcttgaca gcggggctgg tttgtttcta gaagacaaaa     120

agtggggaat catttttacg taactccccc tgataagaag gactcacatc cttataggta     180

cgataaagaa tggttgtatc tttcctattt ttcgaaatcg ttatcttata tagttgaact     240

actacggtta aaaagcttaa gcctcagccc tcttaatcaa acttcttttt tgaaggcacc     300

agggtgcata aaagtgcgtc tattgtttcc cagtggaact ctgttgagat agcgatgttt     360

gttttttttt acttaacggc aaccaatacc gatagcgacg tcgctggcag tgtagagtgg     420

ccgtacggcg tcgctagatg gcacggcact aattgcggcg ggagtcgcta ggcggtgatg     480

catttccgca cagggaccag aggaagcttc ccaggcggtg acagtaagtg aactcattat     540

catgtcttct ccaaaacatt cgtgacatct agtcatgctc ctcgcaattc actccgattg     600

gtatagcttt ttcggtagtt ttagctacta tgcttagggg aaagaggaga aaccgtaccg     660

tcagtctcag tcaaaaaatt ttgatattca atctgatagc aaagttggaa cttggggtta     720

tctggccctt ttttgttatc atattcgtat acccaacaac atatcggttc caccggtcct     780

ttttatatat aaaagacgat gtgtagatgc actcgagtat tcttggagaa cgtgacttgt     840

attgagctag agtgctggat aaagtaccac atattaacgt tcttttatag agccaaacac     900

aattcttttg cactttcaac ataaggtaca actgaaaaca caggaaaaaa agaactaact     960

ctaagta                                                               967


<210> 95
<211> 1000
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_TIR3 promoter

<400> 95
gttcgaggcc aaacaaaaac cgcagcacaa tggagtaaaa gtcgaaggca cgggccccaa      60

acgatagagt ggaagttgta ggaaacaaaa gtggccgtct atgtggcgct tctctgcggt     120

gaattctgtg cgaagtgcct aaacgaaaac ggacggagaa ggctgcgggg ggatatccct     180

tgcataaagg atgcccggca ctgtcctaga cggtgccttc ccttagagac taggagagac     240

ttgagtagga gaaactgagc aggaaaattc gagagataaa gtacatgtgc ctaggtaagc     300

tgtacttcga acagctaata ttagtaagat gcataaaggg gcgtcaaagt ggaaatataa     360

cgacaggaga atctcggtgt gtaagttgga atgtgtattg caccacttcc atgattgggc     420

ttttttcctg gcgcaaaatg ttgctttttc aacatttttc ttatacgcag agagagaatc     480

gtcagatagg tggtaaaact tggagcctaa ttagctcatt atttcatttg aacgagacat     540

tggcttctgc cctacatcac ccatacgaga cattggcctt atacgaggcg tacttcgcct     600

tgcgggaaaa aaaatttctt ttgtaagcga ggttactcag aaactgtgac tgaaggcaga     660

ggttggaaac gtgtgtagac tccaaatgga acgataggaa cgtgtaccct accgcttctg     720

cacacgtttc ttagatatgc gtgccttaag gcgacccatt gttcctgttt accatgattt     780

ctgtaggctt ttttttagaa ggccctagaa tggattgcag atttccgtct gagaaggcag     840

tatacgtaca tatgaatata tatatacata tattctttgg tggagagtat aagcgtttcg     900

tctcctcttc ctttctctac tgcagatttt cttgctggtt gtttgatttt ttctttttag     960

tgttccccag atccaattgt tcaaccgccc acacacaaca                          1000


<210> 96
<211> 1000
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_TIR2 promoter

<400> 96
aaacttaaaa caatccatta cctgcctagt ataatttttt tagaccagaa tatgactcca      60

taaaacgtga agcagcagct agaaaaacga cgcctcggcg gagtttgcgt ttaactctca     120

tgtgcggcca aacaggtgcg taagagctgt gactatttaa ttatgtaata tttacggaaa     180

aaggagtgga caaaaaaggc gtgaatttta ctccactggc ggtagccgcc tataaagaaa     240

ttgataagag gcgtgcctct tatgcgatct tcggaccaac aacaaagcac acaatagcgc     300

aaagtataca caatagagag cctgtaggaa agcagtgggg aatgtcttgg ctttttttat     360

gcccagaaaa aaataaacgt aacgttgggg aactacgcgc aatcatattt catggcctcc     420

ccacggtgct tagagcagcg cgttgcctac agaaacatct ttataaaatc ttggccggcc     480

aagattccca aacgagactc acatttccca aaaaagacat ttctgtccaa aagtagaagg     540

caagaaaacc ctggaggaat cataggcaaa gaaagaaaag aagaagttca tctttaaaac     600

tacctttcaa gcctttattc gttcctcgta aaggacacac gaaaaaaata aacagtacct     660

tgcagaagga gtgcagagtt aggtcgcagg gaatccttga aagccaagag ttttttttcc     720

gtaatgatct cccaaagcaa ccatcaacat tgtggtgcaa agtttagtgt aagatgttct     780

actgaactat cttaatagct gagcatcatg tgagtaaacg agtaagcaag aaaacaacaa     840

agtaatgttc aactttcgta actacggaaa ataatatata agtagttaac gaaattcgaa     900

caatgagagc tctcacatat catcttcttt tccagtttag ccattatcag cacaataata     960

caaaacacac tcgtacactc gcttcaacta taacaaaaaa                          1000


<210> 97
<211> 1000
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_HEM13 promoter

<400> 97
aaaggtagaa ggtattgcac cgattcctag tatcaattct tttcaaaacc acttattttg      60

caaaacatgc gtctaacctg ccatttcagc aacgtaaata ataattactt ccatatatat     120

tcttctcccc cagtagcaat tttgacatga caaatttcag cgatagttgc ttcggcattc     180

gaaaatcgta gaagtatgtc ttaatgtaga aggttgagaa caaccggatc ttgcggtcat     240

ttttcttttc gaggaaagtg caagtctgcc actttccaga aggcatagcc ttgccctttt     300

gttgatattt ctccccaccg taattgttgc attcgcgatc ttttcaacaa tacattttat     360

catcaagccc gcaaatcctc tggagtttgt cctctcgttc actgttggga aaaacaatac     420

gcctaattcg tgattaagat tcttcaaacc atttcctgcg gagtttttac tgtgtgttga     480

acggttcaca gcgtaaaaaa aagttactat aggcacggta ttttaatttc aattgtttag     540

aaagtgcctt cacaccatta gcccctggga ttaccgtcat aggcactttc tgctgagctc     600

ctgcgagatt tttgcgctga aagagtaaaa gaaatctttc acagcggctc cgcgggccct     660

tctactttta aacgagtcgc aggaacagaa gccaaatttc aaagaacgct acgctttcgc     720

cgtttctggt tctcccacca ataacgctcc agcttgaaca aagcataaga ctgcaaccaa     780

agcgctgacg gacgatccga agataaagct tgctttgccc attgttctcg tttcgaaagg     840

ctatataagg acacggattt tccttttttt ttccacctat tgtctttctt tgttaagctt     900

ttattctccg ggtttttttt ttgagcatat cgaaagcttt cttttcgcaa atcaaacata     960

gcaaaccgaa ctcttcgaac acaattaaat acacataaaa                          1000


<210> 98
<211> 1000
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_YHK8 promoter

<400> 98
acagcggtac caagtgacac attgcgtact gtgtagtatg cgccgtatat aacttttttt      60

ttctgaaggt actttgaatt acaatctatt ttttacagtt cctatggcag gggttgaaga     120

tatttgggtc tgaaccatag caggattagt gttatagtag gtatgtgaat agaagctaac     180

aaaatgagat gaacctcata caaagtcgta gagaaaactg ctaacagaag agctgcgcct     240

tgaaatcgta tctctaagct tataataaat tgaaaggaaa aaatacgtgg taaatgcaag     300

cgaccaaaag gctacggccc aacgctaacc cgccgatagg tgcataatct aatttacctc     360

caccagcagg agcccttttt ctaagtaata agcaaaccag ataacttaca tcttgctgta     420

ggaaacaaaa gccggaataa tggttcactc atattcttcg tgtgaaacac agaagaaatc     480

caatatttgc ttcagtattt atctctaaaa attggtccta cattggaaac cataaaccaa     540

ttataaccgg tgtacgaatt gtaagctagt tctggaaatg tcatgttgcg caggtaaaag     600

tggagctgaa ttgtatatct gttttgatca ttattatccc tctgggtgag tggaaatatc     660

aataaaatgc aatggcacat ttaatatcct tctcttaatt ccgtgattta taacatcttg     720

atgccagaaa cacctttcgg atccggcaat aaagcggaga ttagcacgct tttcgccggt     780

cctacggatt tagtgttggc tattgttgag attagtaata cgcagagaat ttttctaccg     840

gtgaagcgac catctcagat tattaggtca agcaataaca cttgttatta gatgcgttac     900

gttcacctgg accctatata aaggcatatt tgccttacat ttggtgtatg gtatcacctt     960

gtatactaag aacatatata tgcagatata acaacaggat                          1000


<210> 99
<211> 1000
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_FET4 promoter

<400> 99
ctgtgcttgc tgttctaacc atacgtacta ctacgttgat tggattataa tgtcctttgg      60

gtattctccg attacgttgt cccatttttc ttttttgcct tgttgcgaag agcgttgttc     120

cgaaaaccgg gtgcactttt tgttcacttt atatatccca cgaggagact gcgcgaacat     180

gatatgtttg acctggcata gtgctattgt tcataaacaa taagccccct aaatacctgc     240

atgtacgaag gtgtacgtac ggtcattcgc atttccctag cattactttc agtactggac     300

aggttatttt tacccctcta gtttgcgact accactaaat gggccatttt tacgtgtcta     360

cgacatatcg caagggtttt tttcttttgt cttattcgag taattatata agcgcgcgta     420

gtcatttttt tagcgacacc ctagggtcct gtagcagtaa tttgaggttt tcatggctcg     480

aacaacgaca aaatattgag gaatcttgag ccttattggg gcgtaaatca cacaggtgtt     540

gaatgagctt ggagcttgtg cggttatgta ttagatatgg gcagtttcct ttaacgttgg     600

cgcctctttc tttcctaccc gtggggttgc tctcgaaact gattaggtca tgagcatttt     660

tgctgagtct ttttaggaag cagcattttt ctcaaacgaa aaaaaaaaaa accaaaaaaa     720

aaaataaaga caaaaaaact atataatgct actggccttg aacttgcttg gtagaagcct     780

tcttaattgc acccattagt ttagcatcta ccacatcttg attcgatgct cttcatttag     840

aaatacttgt ttatataaat atacttcaca cggcatgaat taattgatag gtaagtaagt     900

ttcttttatc ataagtcaaa cagtatcaga tataagaagg gaaaaggtag atatagaaac     960

ctaataagac ccttattatt aaacattcgt taatcaaata                          1000


<210> 100
<211> 1000
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_TIR4 promoter

<400> 100
atgaagtaga cacgataaag ttcttgaaga aagattgtgg atgggtttgc tgaagcttag      60

aactaagaaa agaaacaagc acgcgcaaga aaaccgctac ggctaacttc taaggtgagg     120

atattgtgat tgtttatgcc tcatattttg ctttttatat gttcttcctt catacggcgt     180

gcaaattatc cgccggcgaa cagggtgcta ttctaagagg aactttgaaa ctgtaaacgc     240

ggtgtactga aggggtcatc tgaacgaaca atattttatg cttttgttag ccaaacggta     300

ctgataaggg actgtgaaaa aaaaagacaa tgtatggggt tcacggtttc ccagattcgt     360

gtgtgtgtaa taattcgttt acggcgcacg acagacactt caggaaattt aaagatcaga     420

ataggcatac actccacgca aggaaaacaa aagttgctac cgtaaccggc tcgtacgggg     480

aaagcaaggt catctagtgc tcacttacga actggcataa tcgagttgtg acttccaaac     540

ttaaccgtac tctcacagct gatgtgatcc gaagaaaacc ctgaagatac aacaatggca     600

accaaaggca acttaaaaag gcaaacgaag tacttttcaa caatagctta cactgaaact     660

cgcggagagg cgacgctgcg cgcgaattat cagtaaaaat ctcgtaacaa cccatctgcc     720

acttttagaa ggaatggaaa ttctcaaaaa atgacggaaa ataatagaac agggttccta     780

aacgatgcat ttctaacaag taggatagtc caaaagggag gatgcaaatt ctttgttttc     840

cacgcagtaa gatatgatcc caagctttcg aatataaaaa gggaaggatc gctaggacag     900

atcagttttt agcccgttta cttatctcgt tccctactct tgttctgata gaaaccagca     960

acaaaaacct attcactcgc ttattaatac cataaaaaaa                          1000


<210> 101
<211> 600
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_AAC3 promoter

<400> 101
gatgcctatc gtagtttgca aggaagacgg cttccaactg acaccgccct agtctggcag      60

gattcgtgaa aggagtccat gccagctgct acccagggat tgccacggcc ccggccagcg     120

tatagtacac tgtcaggagc aattcgaaga ggcaaccaaa attatgctaa gcatcgcact     180

ctctctcagt ctggtgctaa gcggaagaca tgcttccaat ggcctcctca ccgagaacgg     240

atattaaacg aaacgaagaa aaaaaatctt cacggaaaat gcgtaatgtc tggatgacaa     300

aatgcatggg tgtaaaaaag gaaatgagac gaacttgtaa taaaccttag tgggttgacg     360

aattttgaaa taaagttttt cctttttttt tttttttttc tttttcattg tttggttgcc     420

ttcaaattac atataagatt tctcgagaag ggttttccat tgtccttttc attaggcgtt     480

gaagtgaatc taaagtgcgc ttgaatgatt tcagatagaa agactaaaga agtggtgtga     540

gtataattaa ctcaattgaa gacggtttac ctgaagtgat atactgtgcc ttgagaaaca     600


<210> 102
<211> 300
<212> DNA
<213> Saccharomyces cerevisiae


<220> 
<223> Sc_PGK1 terminator

<400> 102
aattgaattg aattgaaatc gatagatcaa tttttttctt ttctctttcc ccatccttta     60

cgctaaaata atagtttatt ttattttttg aatatttttt atttatatac gtatatatag    120

actattattt atcttttaat gattattaag atttttatta aaaaaaaatt cgctcctctt    180

ttaatgcctt tatgcagttt ttttttccca ttcgatattt ctatgttcgg gttcagcgta    240

ttttaagttt aataactcga aaattctgcg ttcgttaaag ctttcgagaa ggatattatt    300


<210> 103
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligononucleotide primer Fw-PRK (DBC-15631)

<400> 103
ccccggtctc gaatgtcaca acaacaaaca attgtg                               36


<210> 104
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligononucleotide primer Rv-PRK (DBC-15632)

<400> 104
ggggggtctc ctttaggctt tagcagctgt tgcag                                35


<210> 105
<211> 486
<212> DNA
<213> Artificial Sequence


<220> 
<223> gBLOCK bearing sequence of SNR52p-gRNA.INT1-SUP4t

<400> 105
ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcccgcgg tctttgaaaa      60

gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt ttctttcgag     120

tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt agtgccctct     180

tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt caaaagattt     240

tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga aacttctccg     300

cagtgaaaga taaatgatct attagaacca gggaggtccg ttttagagct agaaatagca     360

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt     420

tttttgtttt ttatgtctcc gcggcaatta accctcacta aagggaacaa aagctggagc     480

tccacc                                                                486


<210> 106
<211> 5949
<212> DNA
<213> Artificial Sequence


<220> 
<223> pRN599

<400> 106
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc      240

accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca      300

ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat      360

taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc      420

ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc      480

aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt      540

agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg      600

tttctattat gaatttcatt tataaagttt atgtacacct aggatccgtc gacactggat      660

ggcggcgtta gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc      720

atgatattac tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa      780

aaatataaat cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata      840

caaatgacaa gttcttgaaa acaagaatct ttttattgtc agtactgatt agaaaaactc      900

atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg      960

aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag     1020

atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc     1080

ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga     1140

gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc     1200

gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag     1260

acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg     1320

caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac     1380

ctggaatgct gttttgccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg     1440

gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat     1500

ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc     1560

atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc     1620

ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tcgaaacgtg     1680

agtcttttcc ttacccatgg ttgtttatgt tcggatgtga tgtgagaact gtatcctagc     1740

aagattttaa aaggaagtat atgaaagaag aacctcagtg gcaaatccta accttttata     1800

tttctctaca ggggcgcggc gtggggacaa ttcaacgcgt ctgtgagggg agcgtttccc     1860

tgctcgcagg tctgcagcga ggagccgtaa tttttgcttc gcgccgtgcg gccatcaaaa     1920

tgtatggatg caaatgatta tacatgggga tgtatgggct aaatgtacgg gcgacagtca     1980

catcatgccc ctgagctgcg cacgtcaaga ctgtcaagga gggtattctg ggcctccatg     2040

tcgctggccg ggtgacccgg cggggacgag gccttaagtt cgaacgtacg agctccggca     2100

ttgcgaatac cgctttccac aaacattgct caaaagtatc tctttgctat atatctctgt     2160

gctatatccc tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc     2220

gacctctaca ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta     2280

agaactattc atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag     2340

tatatagaga caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa     2400

cgctatcact ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg     2460

gatgccttta tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa     2520

gtggagtcag gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc     2580

ttaacggacc tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag     2640

aaaaaaagta atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta     2700

gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg     2760

ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt     2820

ttgttttaca aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat     2880

ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg     2940

catttttgtt ctacaaaatg aagcacagat gcttcgttaa caaagatatg ctattgaagt     3000

gcaagatgga aacgcagaaa atgaaccggg gatgcgacgt gcaagattac ctatgcaata     3060

gatgcaatag tttctccagg aaccgaaata catacattgt cttccgtaaa gcgctagact     3120

atatattatt atacaggttc aaatatacta tctgtttcag ggaaaactcc caggttcgga     3180

tgttcaaaat tcaatgatgg gtaacaagta cgatcgtaaa tctgtaaaac agtttgtcgg     3240

atattaggct gtatctcctc aaagcgtatt cgaatatcat tgagaagctg cagcgtcaca     3300

tcggataata atgatggcag ccattgtaga agtgcctttt gcatttctag tctctttctc     3360

ggtctagcta gttttactac atcgcgaaga tagaatctta gatcacactg cctttgctga     3420

gctggatcaa tagagtaaca aaagagtggt aaggcctcgt taaaggacaa ggacctgagc     3480

ggaagtgtat cgtacagtag acggagtata ctaggtatag tctatagtcc gtggaattaa     3540

ttctcatgtt tgacagctta tcatcgataa tccggagcta gcatgcggcc gctctagaac     3600

tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga     3660

gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt     3720

aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca     3780

taggagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat     3840

taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt     3900

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct     3960

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa     4020

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa     4080

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc     4140

tcggcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga     4200

caggactata aagataccag gcgttccccc ctggaagctc cctcgtgcgc tctcctgttc     4260

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt     4320

ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct     4380

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg     4440

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta     4500

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct     4560

acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa     4620

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt     4680

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta     4740

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat     4800

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa     4860

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct     4920

cagcgatctg tctatttcgt tcatccatag ttgcctgact gcccgtcgtg tagataacta     4980

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct     5040

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg     5100

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa     5160

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt     5220

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta     5280

catgatcccc catgttgtga aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca     5340

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta     5400

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct     5460

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg     5520

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac     5580

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact     5640

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa     5700

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt     5760

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat     5820

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg     5880

acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc     5940

cctttcgtc                                                             5949


<210> 107
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer INT1-5'flank (BOZ-783)

<400> 107
cggcattatt gtgtatggc                                                 19


<210> 108
<211> 72
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer INT1-5 'flank with connector c (DBC-19944)

<400> 108
caacaggagg cggatggata tactgtggtc tggaagatgc cggaaagcgt catgttatgc     60

tcctcaatca cg                                                         72


<210> 109
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer connector c to amplify anaerobic promoter-PRK-PGK1
      terminator expression cassette (DBC-5799)

<400> 109
acgctttccg gcatcttcca g                                              21


<210> 110
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer connector d to amplify PRK expression cassette
      (DBC-5800)

<400> 110
gcggaatatt ggcggaacgg                                                20


<210> 111
<211> 74
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer to amplify URA3 marker with flank connector d
      (DBC-19947)

<400> 111
aacgttgtcc aggtttgtat ccacgtgtgt ccgttccgcc aatattccgc gctgtggttt     60

cagggtccat aaag                                                       74


<210> 112
<211> 72
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer to amplify URA3 locus with flank connector e
      (DBC-19949)

<400> 112
aggtacaaca agcacgaccg agcatatggg aaggatgttt gtggttattt cgagattccc     60

gggtaataac tg                                                         72


<210> 113
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer INT1-3'flank with connector d (DBC-19946)

<400> 113
aactccatca aatggtcagg                                                20


<210> 114
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer INT1-3 'flank (BoZ-788)

<400> 114
aacaaaagct gggtaccggg                                                20


<210> 115
<211> 73
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer on backbone pRN599 (DBC-13775)

<400> 115
cttatcgata ccgtcgacct cgaggggggg cccggtaccc agcttttgtt ccgcggtctt     60

tgaaaagata atg                                                        73


<210> 116
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer on backbone pRN599 (DBC-13776)

<400> 116
aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt ccgcggagac     60

ataaaaaac                                                             69


<210> 117
<211> 73
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer on gRNA cassette INT1 (DBC-13773)

<400> 117
cttatcgata ccgtcgacct cgaggggggg cccggtaccc agcttttgtt ccgcggtctt     60

tgaaaagata atg                                                        73


<210> 118
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer on gRNa cassette INT1 (DBC-13774)

<400> 118
aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt ccgcggagac     60

ataaaaaac                                                             69


<210> 119
<211> 82
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer 7933 YEN1p prk cassette construction

<400> 119
tcacagaggg atcccgttac ccatctatgc tgaagattta tcatactatt cctccgctcg     60

ggaattcaag caatagcgtt tc                                              82


