                         SEQUENCE LISTING

<110>  Henkel AG & Co. KGaA
 
<120>  Bacillus gibsonii Protease und Varianten davon

<130>  PT033917WO

<150>  102016210628.7
<151>  2016-06-15

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  269
<212>  PRT
<213>  Bacillus gibsonii

<400>  1

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Met Ser Leu Gly Ser Asp Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Asn Gly Thr Gly Ser Ile Gly 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Met Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


<210>  2
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease Variante

<400>  2

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Val Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Met Ser Leu Gly Ser Asp Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Asn Gly Thr Gly Ser Ile Gly 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Met Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


<210>  3
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease Variante

<400>  3

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Leu Ser Leu Gly Ser Asp Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Ser Gly Ala Gly Ser Ile Gly 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Met Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


<210>  4
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease Variante

<400>  4

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Met Ser Leu Gly Ser Asp Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Asn Gly Thr Gly Ser Ile Gly 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Asn Asp Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


<210>  5
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease Variante

<400>  5

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Met Ser Leu Gly Ser Asp Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Asn Gly Thr Gly Ser Ile Gly 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Leu Asp Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


<210>  6
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease Variante

<400>  6

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Met Ser Leu Gly Ser Asp Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Asn Gly Thr Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Met Pro Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


<210>  7
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease Variante

<400>  7

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Met Ser Leu Gly Ser Pro Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Asn Gly Thr Gly Ser Ile Gly 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Leu Asp Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


<210>  8
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease Variante

<400>  8

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Met Ser Leu Gly Ser Asp Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Asn Gly Thr Gly Ser Ile Gly 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Met His Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


<210>  9
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease Variante

<400>  9

Gln Gln Thr Val Pro Trp Gly Ile Thr Arg Val Leu Ala Pro Thr Val 
1               5                   10                  15      


His Asn Arg Gly Ile Thr Gly Ser Gly Val Lys Val Ala Ile Leu Asp 
            20                  25                  30          


Thr Gly Ile Ala Gln His Ser Asp Leu Thr Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Ser Thr Thr Ala Asp Leu Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly Val Ile 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Asp Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Arg Gly Ser Val Ser Gly Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Ala Thr Asn Asn Met His Ile Ala Asn Leu Ser Leu Gly Ser Asp Ala 
        115                 120                 125             


Pro Ser Thr Thr Leu Glu Arg Ala Val Asn Tyr Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Ile Ala Ala Thr Gly Asn Asn Gly Thr Gly Ser Ile Gly 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Arg Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Ile Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Leu Asn Asn Ser Tyr 
        195                 200                 205             


Ala Ser Met Pro Gly Thr Ser Met Ala Thr Pro His Val Ser Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Asn Ala Thr Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Ser Ser Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Asp Ala Ala Thr Arg 
            260                 265                 


