                         SEQUENCE LISTING

<110>  THE UNIVERSITY OF CHICAGO
 
<120>  EVOLUTION OF A PROXIMITY-DEPENDENT SPLIT RNA POLYMERASE AS A 
       VERSATILE BIOSENSOR PLATFORM

<130>  ARCD-P0618WO

<150>  62/346,174
<151>  2016-06-06

<150>  62/424,875
<151>  2016-11-21

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  2115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Unknown Organism

<400>  1
aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag       60

gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg      120

ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa      180

gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt      240

gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact      300

ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact      360

cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg      420

attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac      480

gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa      540

ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt      600

gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag      660

ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac      720

atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg      780

accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg      840

ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc      900

aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac      960

acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct     1020

ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct     1080

tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt     1140

aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac     1200

gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat     1260

gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa     1320

tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac     1380

gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt     1440

gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag     1500

ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt     1560

aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt     1620

cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac     1680

aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct     1740

accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct     1800

cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac     1860

gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct     1920

gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt     1980

gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac     2040

aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac     2100

ttcgcgttcg cgtaa                                                      2115


<210>  2
<211>  704
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism

<400>  2

Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser Lys Gly Leu 
1               5                   10                  15      


Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp Ser Ile His 
            20                  25                  30          


Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr Gly Met Val 
        35                  40                  45              


Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp Ser Glu Thr 
    50                  55                  60                  


Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly 
65                  70                  75                  80  


Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val Val Pro Pro 
                85                  90                  95      


Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg 
            100                 105                 110         


Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala Leu Met Arg 
        115                 120                 125             


Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala 
    130                 135                 140                 


Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala Val Ala Asn 
145                 150                 155                 160 


Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile Pro Ala Ile 
                165                 170                 175     


Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp Met Asn Pro 
            180                 185                 190         


Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys 
        195                 200                 205             


Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe Met Leu Glu 
    210                 215                 220                 


Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe Pro Tyr Asn 
225                 230                 235                 240 


Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe Asn Pro Gln 
                245                 250                 255     


Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro 
            260                 265                 270         


Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly Ala Asn Cys 
        275                 280                 285             


Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu 
    290                 295                 300                 


Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro Leu Glu Asn 
305                 310                 315                 320 


Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys 
                325                 330                 335     


Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr Asn Cys Ser 
            340                 345                 350         


Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln His Phe Ser 
        355                 360                 365             


Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn Leu Leu Pro 
    370                 375                 380                 


Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys Lys Val Asn 
385                 390                 395                 400 


Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn Glu Val Val 
                405                 410                 415     


Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys Val Lys Leu 
            420                 425                 430         


Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg 
        435                 440                 445             


Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly Ser Lys Glu 
    450                 455                 460                 


Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln Pro Ala Ile 
465                 470                 475                 480 


Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln Ala Ala Gly 
                485                 490                 495     


Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr Val Val Ala 
            500                 505                 510         


Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala 
        515                 520                 525             


Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys 
    530                 535                 540                 


Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr 
545                 550                 555                 560 


Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu Gly Gln Phe 
                565                 570                 575     


Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala 
            580                 585                 590         


His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His Ser Gln Asp 
        595                 600                 605             


Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu Lys Tyr Gly 
    610                 615                 620                 


Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr Ile Pro Ala 
625                 630                 635                 640 


Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met Val Asp Thr 
                645                 650                 655     


Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp 
            660                 665                 670         


Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu Pro Ala Lys 
        675                 680                 685             


Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala 
    690                 695                 700                 


<210>  3
<211>  537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Unknown Organism

<400>  3
atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg       60

ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag      120

catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa      180

gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag      240

atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg      300

acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag      360

accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca      420

atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag      480

cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaag         537


<210>  4
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism

<400>  4

Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu 
            20                  25                  30          


Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
    50                  55                  60                  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  


Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      


Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu 
            100                 105                 110         


Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser 
        115                 120                 125             


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala 
    130                 135                 140                 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     


Val Tyr Lys 
            


<210>  5
<211>  537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Unknown Organism

<400>  5
atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg       60

ttcaacactc tggctgacca ttacggtgag cgttcagctc gcggacagtt ggcccttgag      120

catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa      180

gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag      240

atgattgccc gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caggcgcccg      300

acagccttcc agttcctaaa agaaatcaag ccggaagccg tagcgtacat caccattaag      360

acctctctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca      420

atcggtcgga ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag      480

cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaag         537


<210>  6
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism

<400>  6

Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Ser 
            20                  25                  30          


Ala Arg Gly Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
    50                  55                  60                  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  


Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      


Gly Arg Arg Pro Thr Ala Phe Gln Phe Leu Lys Glu Ile Lys Pro Glu 
            100                 105                 110         


Ala Val Ala Tyr Ile Thr Ile Lys Thr Ser Leu Ala Cys Leu Thr Ser 
        115                 120                 125             


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Thr 
    130                 135                 140                 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     


Val Tyr Lys 
            


<210>  7
<211>  537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Unknown Organism

<400>  7
atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg       60

ttcaacactc tggctgacca ttacggtgag cgttcagctc gcggacagtt ggcccttgag      120

catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa      180

gctggtaagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag      240

atgattgccc gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caggcgcccg      300

acagccttcc agttcctaaa agaaatcaag ccggaagccg tagcgtacat caccattaag      360

acctctctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca      420

atcggtcgga ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag      480

cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaag         537


<210>  8
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism

<400>  8

Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Ser 
            20                  25                  30          


Ala Arg Gly Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Lys Val 
    50                  55                  60                  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  


Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      


Gly Arg Arg Pro Thr Ala Phe Gln Phe Leu Lys Glu Ile Lys Pro Glu 
            100                 105                 110         


Ala Val Ala Tyr Ile Thr Ile Lys Thr Ser Leu Ala Cys Leu Thr Ser 
        115                 120                 125             


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Thr 
    130                 135                 140                 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     


Val Tyr Lys 
            


<210>  9
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism


<220>
<221>  RNAP(1-179)
<222>  (1)..(1)

<400>  9

Gly Gly Ser Gly Ser Gly Ser Ser Ala Leu Lys Lys Glu Leu Gln Ala 
1               5                   10                  15      


Asn Lys Lys Glu Leu Ala Gln Leu Lys Trp Glu Leu Gln Ala Leu Lys 
            20                  25                  30          


Lys Glu Leu Ala Gln 
        35          


<210>  10
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism


<220>
<221>  RNAP(180+)
<222>  (39)..(39)

<400>  10

Met Ala Ser Glu Gln Leu Glu Lys Lys Leu Gln Ala Leu Glu Lys Lys 
1               5                   10                  15      


Leu Ala Gln Leu Glu Trp Lys Asn Gln Ala Leu Glu Lys Lys Leu Ala 
            20                  25                  30          


Gln Thr Ser Gly Gly Ser Gly 
        35                  


<210>  11
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism


<220>
<221>  RNAP(180+)
<222>  (39)..(39)

<400>  11

Met Ala Ser Glu Gln Leu Glu Lys Glu Leu Gln Ala Leu Glu Lys Glu 
1               5                   10                  15      


Leu Ala Gln Leu Lys Trp Lys Asn Gln Ala Leu Glu Lys Lys Leu Ala 
            20                  25                  30          


Gln Thr Ser Gly Gly Ser Gly 
        35                  


<210>  12
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism


<220>
<221>  N-28-1(1-179)
<222>  (1)..(1)

<400>  12

Gly Gly Ser Gly Ser Gly Ser Ser Ala Leu Lys Lys Glu Leu Gln Ala 
1               5                   10                  15      


Asn Lys Lys Glu Ile Ala Gln Leu Lys Trp Glu Ile Gln Ala Leu Lys 
            20                  25                  30          


Lys Glu Leu Ala Gln 
        35          


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism


<220>
<221>  N-28-1(1-179)
<222>  (1)..(1)

<220>
<221>  iLID
<222>  (8)..(8)

<400>  13

Gly Gly Ser Gly Ser Gly Ser Ser 
1               5               


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism


<220>
<221>  SspBnano
<222>  (1)..(1)

<220>
<221>  RNAP(180+)
<222>  (6)..(6)

<400>  14

Thr Ser Gly Gly Ser Gly 
1               5       


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism


<220>
<221>  N-28-1(1-179)
<222>  (1)..(1)

<220>
<221>  FRB
<222>  (8)..(8)

<400>  15

Gly Gly Ser Gly Ser Gly Ser Ser 
1               5               


<210>  16
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Unknown Organism


<220>
<221>  FKBP
<222>  (1)..(1)

<220>
<221>  RNAP(180+)
<222>  (6)..(6)

<400>  16

Thr Ser Gly Gly Ser Gly 
1               5       


