               SEQUENCE LISTING

<110> INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE)
      ICM (INSTITUT DU CERVEAU ET DE LA MOELLE EPINIERE)
      CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
      UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)
      COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES
      ALTERNATIVES (CEA)

<120> Diet controlled expression of a nucleic acid encoding Cas9 nuclease and uses thereof

<130> PR77982

<150> EP16172964
<151> 2016-06-03

<160> 12

<170> BiSSAP 1.3.2

<210> 1
<211> 16
<212> DNA
<213> Artificial Sequence

<223> "AARE sequence from the TRIB3 gene"

<220> 
<223> AARE sequence from the TRIB3 gene

<400> 1
cggtttgcat cacccg                                                    16


<210> 2
<211> 16
<212> DNA
<213> Artificial Sequence

<223> "AARE sequence from the CHOP gene"

<220> 
<223> AARE sequence from the CHOP gene

<400> 2
aacattgcat catccc                                                    16


<210> 3
<211> 16
<212> DNA
<213> Artificial Sequence

<223> "AARE sequence from the ASNS gene"

<220> 
<223> AARE sequence from the ASNS gene

<400> 3
gaagtttcat catgcc                                                    16


<210> 4
<211> 16
<212> DNA
<213> Artificial Sequence

<223> "AARE sequence from the ATF3 gene"

<220> 
<223> AARE sequence from the ATF3 gene

<400> 4
agcgttgcat cacccc                                                    16


<210> 5
<211> 16
<212> DNA
<213> Artificial Sequence

<223> "AARE sequence from the SNAT2 gene"

<220> 
<223> AARE sequence from the SNAT2 gene

<400> 5
gatattgcat cagttt                                                    16


<210> 6
<211> 94
<212> DNA
<213> Artificial Sequence

<223> "Thymidine kinase minimal promoter nucleic acid"

<220> 
<223> Thymidine kinase minimal promoter nucleic acid

<400> 6
cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca     60

gcgacccgct taacagcgtc aacagcgtgc cgca                                 94


<210> 7
<211> 215
<212> DNA
<213> Artificial Sequence

<223> "2XAARE nucleic acid"

<220> 
<223> 2XAARE nucleic acid

<400> 7
gattagctcc ggtttgcatc acccggaccg ggggattagc tccggtttgc atcacccgga     60

ccgggggatt agctccggtt tgcatcaccc ggaccggggg ccgggcgcgt gctagcgatt    120

agctccggtt tgcatcaccc ggaccggggg attagctccg gtttgcatca cccggaccgg    180

gggattagct ccggtttgca tcacccggac cgggg                               215


<210> 8
<211> 4212
<212> DNA
<213> Artificial Sequence

<223> "NLS-FLAG CAS9 nucleic acid"

<220> 
<223> NLS-FLAG CAS9 nucleic acid

<400> 8
atgggaccta agaaaaagag gaaggtgcct aagaaaaaga ggaaggtgcc taagaaaaag       60

aggaaggtgg cggccgctga ctacaaggat gacgacgata aatctagaga caagaaatac      120

tctattggac tggatatcgg gacaaactcc gttggctggg ccgtcataac cgacgagtat      180

aaggtgccaa gcaagaaatt caaggtgctg ggtaatactg accgccattc aatcaagaag      240

aacctgatcg gagcactcct cttcgactcc ggtgaaaccg ctgaagctac tcggctgaag      300

cggaccgcaa ggcggagata cacccgccgc aagaatcgga tatgttatct gcaagagatc      360

tttagcaacg aaatggctaa ggtggacgac tccttctttc accgcctgga agagagcttt      420

ctggtggagg aggataagaa acacgagagg caccctatat tcggaaatat cgtggatgag      480

gtggcttacc atgaaaagta tcctacaatc taccatctga ggaagaagct ggtggacagc      540

accgataaag cagacctgag gctcatctat ctggccctgg ctcatatgat aaagtttaga      600

ggacactttc tgatcgaggg cgacctgaat cccgataatt ccgatgtgga taaactcttc      660

attcaactgg tgcagacata taaccaactg ttcgaggaga atcccataaa cgcttctggt      720

gtggatgcca aggctattct gtccgctcgg ctgtccaagt cacgcagact ggagaatctg      780

attgcccaac tgccaggaga aaagaagaac ggcctgtttg ggaacctcat cgccctgagc      840

ctgggcctga cacctaactt caagtccaat tttgatctgg ccgaagatgc taaactccag      900

ctctccaagg acacctatga cgatgatctg gacaacctgc tcgcacagat aggcgaccag      960

tacgccgatc tctttctggc tgctaagaat ctctccgacg ccattctgct gagcgacata     1020

ctccgggtca acactgagat caccaaagca cctctgagcg cctccatgat aaaacgctat     1080

gatgaacacc atcaagacct gactctgctc aaagccctcg tgaggcaaca gctgccagag     1140

aagtacaaag agatattctt cgaccagagc aagaatggat atgccggata catcgatggc     1200

ggagcatcac aggaagaatt ttacaagttc atcaaaccaa tcctcgagaa gatggacggt     1260

actgaagagc tgctggtgaa gctgaacagg gaggacctgc tgaggaagca gaggaccttt     1320

gataatggct ccattccaca tcagatacac ctgggagagc tgcatgcaat cctccgcagg     1380

caggaggatt tctatccttt cctgaaggat aaccgggaga agatagagaa gatcctgacc     1440

ttcaggatcc cttattacgt cggccctctg gctagaggca actcccgctt cgcttggatg     1500

accaggaaat ctgaggagac aattactcct tggaacttcg aagaggtcgt ggataagggc     1560

gcaagcgccc agtcattcat cgaacggatg accaatttcg ataagaacct gcccaacgag     1620

aaggtcctgc ccaaacattc actcctgtac gagtatttca ccgtctataa cgagctgact     1680

aaagtgaagt acgtgaccga gggcatgagg aagcctgcct tcctgtccgg agagcagaag     1740

aaggctatcg ttgatctgct cttcaagact aatagaaagg tgacagtgaa gcagctcaag     1800

gaggattact ttaagaagat cgaatgcttt gactcagtgg aaatctctgg cgtggaggac     1860

cgctttaatg ccagcctggg cacttaccat gatctgctga agataatcaa agacaaagat     1920

ttcctcgata atgaggagaa cgaggacatc ctggaagata tcgtgctgac cctgactctg     1980

ttcgaggata gagagatgat cgaagagcgc ctgaagacct atgcccatct gtttgacgat     2040

aaagtcatga aacagctcaa gcggcggcgc tacactgggt ggggtagact ctccaggaaa     2100

ctcataaacg gcatccgcga caaacagagc ggaaagacca tcctggattt cctgaaatcc     2160

gacggattcg ctaacaggaa cttcatgcaa ctgattcacg atgactctct gacatttaaa     2220

gaggacatcc agaaggcaca ggtgagcggt caaggcgaca gcctgcacga gcacatcgcc     2280

aacctcgctg gatcacccgc cataaagaag ggaatactgc agacagtcaa ggtcgtggac     2340

gaactcgtca aagtgatggg tcggcacaag ccagagaata tcgttatcga aatggcaagg     2400

gagaaccaaa ccacccagaa gggccagaag aactctcggg aacggatgaa aagaatcgaa     2460

gagggaatta aggagctggg atctcagata ctgaaggagc accctgtgga gaatacacag     2520

ctccagaacg agaaactcta cctgtactac ctccagaacg ggcgggacat gtacgttgac     2580

caggaactcg acatcaaccg gctgtccgat tatgacgtgg accatattgt tccacagtcc     2640

ttcctcaaag atgactccat tgacaacaag gtgctgacca gatccgataa gaatcgcggt     2700

aagtctgaca atgttccatc agaagaggtg gtcaagaaga tgaagaatta ctggcggcag     2760

ctcctcaacg ccaaactgat cacccagcgg aagtttgaca atctgactaa ggcagaaaga     2820

ggaggtctga gcgaactcga caaggccggc tttattaaga ggcaactggt cgaaacacgc     2880

cagattacca aacacgtggc acaaatcctc gactctagga tgaacactaa gtacgatgag     2940

aacgataagc tgatcaggga agtgaaagtg ataactctga agagcaagct ggtgtctgac     3000

ttccggaagg actttcaatt ctacaaagtt cgcgaaataa acaattacca tcatgctcac     3060

gatgcctatc tcaatgctgt cgttggcacc gccctgatca agaaataccc taaactggag     3120

tctgagttcg tgtacggtga ctataaagtc tacgatgtga ggaagatgat agcaaagtct     3180

gagcaagaga ttggcaaagc caccgccaag tacttcttct actctaatat catgaatttc     3240

tttaagactg agataaccct ggctaacggc gaaatccgga agcgcccact gatcgaaaca     3300

aacggagaaa caggagaaat cgtgtgggat aaaggcaggg acttcgcaac tgtgcggaag     3360

gtgctgtcca tgccacaagt caatatcgtg aagaagaccg aagtgcagac cggcggattc     3420

tcaaaggaga gcatcctgcc aaagcggaac tctgacaagc tgatcgccag gaagaaagat     3480

tgggacccaa agaagtatgg cggtttcgat tcccctacag tggcttattc cgttctggtc     3540

gtggcaaaag tggagaaagg caagtccaag aaactcaagt ctgttaagga gctgctcgga     3600

attactatta tggagagatc cagcttcgag aagaatccaa tcgatttcct ggaagctaag     3660

ggctataaag aagtgaagaa agatctcatc atcaaactgc ccaagtactc tctctttgag     3720

ctggagaatg gtaggaagcg gatgctggcc tccgccggag agctgcagaa aggaaacgag     3780

ctggctctgc cctccaaata cgtgaacttc ctgtatctgg cctcccacta cgagaaactc     3840

aaaggtagcc ctgaagacaa tgagcagaag caactctttg ttgagcaaca taaacactac     3900

ctggacgaaa tcattgaaca gattagcgag ttcagcaagc gggttattct ggccgatgca     3960

aacctcgata aagtgctgag cgcatataat aagcacaggg acaagccaat tcgcgaacaa     4020

gcagagaata ttatccacct ctttactctg actaatctgg gcgctcctgc tgccttcaag     4080

tatttcgata caactattga caggaagcgg tacacctcta ccaaagaagt tctcgatgcc     4140

accctgatac accagtcaat taccggactg tacgagactc gcatcgacct gtctcagctc     4200

ggcggcgact ag                                                         4212


<210> 9
<211> 13650
<212> DNA
<213> Artificial Sequence

<223> "Nucleic acid of the pTrip-2XAARE-NLS-FLAG-CAS9 plasmid"

<220> 
<223> Nucleic acid of the pTrip-2XAARE-NLS-FLAG-CAS9 plasmid

<400> 9
ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc       60

tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat      120

gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc      180

catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact      240

gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta      300

caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg      360

cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg      420

ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc      480

tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag      540

gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt      600

caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa      660

ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt      720

gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt      780

tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt      840

ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg      900

tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga      960

atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa     1020

gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga     1080

caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa     1140

ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca     1200

ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta     1260

ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac     1320

ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc     1380

gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag     1440

ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga     1500

taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt     1560

agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata     1620

atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag     1680

aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa     1740

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt     1800

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc     1860

cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa     1920

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa     1980

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc     2040

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa     2100

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa     2160

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg     2220

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc     2280

tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg     2340

ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg     2400

agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg     2460

aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat     2520

gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag     2580

aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc     2640

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc     2700

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg     2760

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca     2820

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac     2880

aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt     2940

aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg     3000

cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc     3060

acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg     3120

caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc     3180

tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat     3240

ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga     3300

tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat     3360

tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc     3420

gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga     3480

tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact     3540

gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg     3600

aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac     3660

atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt     3720

cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga     3780

tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg     3840

atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact     3900

ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa     3960

ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta     4020

taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact     4080

gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa     4140

atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag     4200

aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat     4260

gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct     4320

gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac     4380

agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt     4440

aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag     4500

gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga     4560

ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa     4620

tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag     4680

catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa     4740

actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca     4800

tcccaaactt cccaccccat accctattac cactgccaat tacctagtgg tttcatttac     4860

tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat     4920

gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt     4980

gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg     5040

ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca     5100

gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg     5160

catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca     5220

tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct     5280

tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg     5340

gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct     5400

ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa     5460

gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc     5520

tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc     5580

ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg     5640

cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt     5700

ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg     5760

agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa     5820

ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg     5880

ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca     5940

ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa     6000

aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa     6060

agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag     6120

ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt     6180

agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg     6240

agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac     6300

gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct     6360

gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct     6420

ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg     6480

gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa     6540

taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa     6600

caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa     6660

tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac     6720

aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag     6780

aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc     6840

gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga     6900

aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg     6960

ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg     7020

tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta     7080

caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca     7140

ctttggctga tacgcggatc tacgcgtcaa gtttgtacaa aaaagcaggc tccgcggccg     7200

cccccttcac cggtaccgat tagctccggt ttgcatcacc cggaccgggg gattagctcc     7260

ggtttgcatc acccggaccg ggggattagc tccggtttgc atcacccgga ccgggggccg     7320

ggcgcgtgct agcgattagc tccggtttgc atcacccgga ccgggggatt agctccggtt     7380

tgcatcaccc ggaccggggg attagctccg gtttgcatca cccggaccgg ggactcgagg     7440

tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac     7500

ccgcttaaca gcgtcaacag cgtgccgcaa gcttgaattc tgatcagcat tccggtactg     7560

ttggtaaagc caccatggga cctaagaaaa agaggaaggt gcctaagaaa aagaggaagg     7620

tgcctaagaa aaagaggaag gtggcggccg ctgactacaa ggatgacgac gataaatcta     7680

gagacaagaa atactctatt ggactggata tcgggacaaa ctccgttggc tgggccgtca     7740

taaccgacga gtataaggtg ccaagcaaga aattcaaggt gctgggtaat actgaccgcc     7800

attcaatcaa gaagaacctg atcggagcac tcctcttcga ctccggtgaa accgctgaag     7860

ctactcggct gaagcggacc gcaaggcgga gatacacccg ccgcaagaat cggatatgtt     7920

atctgcaaga gatctttagc aacgaaatgg ctaaggtgga cgactccttc tttcaccgcc     7980

tggaagagag ctttctggtg gaggaggata agaaacacga gaggcaccct atattcggaa     8040

atatcgtgga tgaggtggct taccatgaaa agtatcctac aatctaccat ctgaggaaga     8100

agctggtgga cagcaccgat aaagcagacc tgaggctcat ctatctggcc ctggctcata     8160

tgataaagtt tagaggacac tttctgatcg agggcgacct gaatcccgat aattccgatg     8220

tggataaact cttcattcaa ctggtgcaga catataacca actgttcgag gagaatccca     8280

taaacgcttc tggtgtggat gccaaggcta ttctgtccgc tcggctgtcc aagtcacgca     8340

gactggagaa tctgattgcc caactgccag gagaaaagaa gaacggcctg tttgggaacc     8400

tcatcgccct gagcctgggc ctgacaccta acttcaagtc caattttgat ctggccgaag     8460

atgctaaact ccagctctcc aaggacacct atgacgatga tctggacaac ctgctcgcac     8520

agataggcga ccagtacgcc gatctctttc tggctgctaa gaatctctcc gacgccattc     8580

tgctgagcga catactccgg gtcaacactg agatcaccaa agcacctctg agcgcctcca     8640

tgataaaacg ctatgatgaa caccatcaag acctgactct gctcaaagcc ctcgtgaggc     8700

aacagctgcc agagaagtac aaagagatat tcttcgacca gagcaagaat ggatatgccg     8760

gatacatcga tggcggagca tcacaggaag aattttacaa gttcatcaaa ccaatcctcg     8820

agaagatgga cggtactgaa gagctgctgg tgaagctgaa cagggaggac ctgctgagga     8880

agcagaggac ctttgataat ggctccattc cacatcagat acacctggga gagctgcatg     8940

caatcctccg caggcaggag gatttctatc ctttcctgaa ggataaccgg gagaagatag     9000

agaagatcct gaccttcagg atcccttatt acgtcggccc tctggctaga ggcaactccc     9060

gcttcgcttg gatgaccagg aaatctgagg agacaattac tccttggaac ttcgaagagg     9120

tcgtggataa gggcgcaagc gcccagtcat tcatcgaacg gatgaccaat ttcgataaga     9180

acctgcccaa cgagaaggtc ctgcccaaac attcactcct gtacgagtat ttcaccgtct     9240

ataacgagct gactaaagtg aagtacgtga ccgagggcat gaggaagcct gccttcctgt     9300

ccggagagca gaagaaggct atcgttgatc tgctcttcaa gactaataga aaggtgacag     9360

tgaagcagct caaggaggat tactttaaga agatcgaatg ctttgactca gtggaaatct     9420

ctggcgtgga ggaccgcttt aatgccagcc tgggcactta ccatgatctg ctgaagataa     9480

tcaaagacaa agatttcctc gataatgagg agaacgagga catcctggaa gatatcgtgc     9540

tgaccctgac tctgttcgag gatagagaga tgatcgaaga gcgcctgaag acctatgccc     9600

atctgtttga cgataaagtc atgaaacagc tcaagcggcg gcgctacact gggtggggta     9660

gactctccag gaaactcata aacggcatcc gcgacaaaca gagcggaaag accatcctgg     9720

atttcctgaa atccgacgga ttcgctaaca ggaacttcat gcaactgatt cacgatgact     9780

ctctgacatt taaagaggac atccagaagg cacaggtgag cggtcaaggc gacagcctgc     9840

acgagcacat cgccaacctc gctggatcac ccgccataaa gaagggaata ctgcagacag     9900

tcaaggtcgt ggacgaactc gtcaaagtga tgggtcggca caagccagag aatatcgtta     9960

tcgaaatggc aagggagaac caaaccaccc agaagggcca gaagaactct cgggaacgga    10020

tgaaaagaat cgaagaggga attaaggagc tgggatctca gatactgaag gagcaccctg    10080

tggagaatac acagctccag aacgagaaac tctacctgta ctacctccag aacgggcggg    10140

acatgtacgt tgaccaggaa ctcgacatca accggctgtc cgattatgac gtggaccata    10200

ttgttccaca gtccttcctc aaagatgact ccattgacaa caaggtgctg accagatccg    10260

ataagaatcg cggtaagtct gacaatgttc catcagaaga ggtggtcaag aagatgaaga    10320

attactggcg gcagctcctc aacgccaaac tgatcaccca gcggaagttt gacaatctga    10380

ctaaggcaga aagaggaggt ctgagcgaac tcgacaaggc cggctttatt aagaggcaac    10440

tggtcgaaac acgccagatt accaaacacg tggcacaaat cctcgactct aggatgaaca    10500

ctaagtacga tgagaacgat aagctgatca gggaagtgaa agtgataact ctgaagagca    10560

agctggtgtc tgacttccgg aaggactttc aattctacaa agttcgcgaa ataaacaatt    10620

accatcatgc tcacgatgcc tatctcaatg ctgtcgttgg caccgccctg atcaagaaat    10680

accctaaact ggagtctgag ttcgtgtacg gtgactataa agtctacgat gtgaggaaga    10740

tgatagcaaa gtctgagcaa gagattggca aagccaccgc caagtacttc ttctactcta    10800

atatcatgaa tttctttaag actgagataa ccctggctaa cggcgaaatc cggaagcgcc    10860

cactgatcga aacaaacgga gaaacaggag aaatcgtgtg ggataaaggc agggacttcg    10920

caactgtgcg gaaggtgctg tccatgccac aagtcaatat cgtgaagaag accgaagtgc    10980

agaccggcgg attctcaaag gagagcatcc tgccaaagcg gaactctgac aagctgatcg    11040

ccaggaagaa agattgggac ccaaagaagt atggcggttt cgattcccct acagtggctt    11100

attccgttct ggtcgtggca aaagtggaga aaggcaagtc caagaaactc aagtctgtta    11160

aggagctgct cggaattact attatggaga gatccagctt cgagaagaat ccaatcgatt    11220

tcctggaagc taagggctat aaagaagtga agaaagatct catcatcaaa ctgcccaagt    11280

actctctctt tgagctggag aatggtagga agcggatgct ggcctccgcc ggagagctgc    11340

agaaaggaaa cgagctggct ctgccctcca aatacgtgaa cttcctgtat ctggcctccc    11400

actacgagaa actcaaaggt agccctgaag acaatgagca gaagcaactc tttgttgagc    11460

aacataaaca ctacctggac gaaatcattg aacagattag cgagttcagc aagcgggtta    11520

ttctggccga tgcaaacctc gataaagtgc tgagcgcata taataagcac agggacaagc    11580

caattcgcga acaagcagag aatattatcc acctctttac tctgactaat ctgggcgctc    11640

ctgctgcctt caagtatttc gatacaacta ttgacaggaa gcggtacacc tctaccaaag    11700

aagttctcga tgccaccctg atacaccagt caattaccgg actgtacgag actcgcatcg    11760

acctgtctca gctcggcggc gactagtaaa gcggccgggc tcgagtctag aaagggtggg    11820

cgcgccgacc cagctttctt gtacaaagtg gctcgacggt acctttaaga ccaatgactt    11880

acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa    11940

ttcactccca acgaagacaa aatcgtcgag agatgctgca tataagcagc tgctttttgc    12000

ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg    12060

gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg    12120

tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat    12180

ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg    12240

aatatcagag agtgagaggc cttgacatta taatagattt agcaggaatt gaactaggag    12300

tggagcacac aggcaaagct gcagaagtac ttggaagaag ccaccagaga tactcacgat    12360

tctgcacata cctggctaat cccagatcct aaggattaca ttaagtttac taacatttat    12420

ataatgattt atagtttaaa gtataaactt atctaattta ctattctgac agatattaat    12480

taatcctcaa atatcataag agatgattac tattatcccc atttaacaca agaggaaact    12540

gagagggaaa gatgttgaag taattttccc acaattacag catccgttag ttacgactct    12600

atgatcttct gacacaaatt ccatttactc ctcaccctat gactcagtcg aatatatcaa    12660

agttatggac attatgctaa gtaacaaatt acccttttat atagtaaata ctgagtagat    12720

tgagagaaga aattgtttgc aaacctgaat agcttcaaga agaagagaag tgaggataag    12780

aataacagtt gtcatttaac aagttttaac aagtaacttg gttagaaagg gattcaaatg    12840

cataaagcaa gggataaatt tttctggcaa caagactata caatataacc ttaaatatga    12900

cttcaaataa ttgttggaac ttgataaaac taattaaata ttattgaaga ttatcaatat    12960

tataaatgta atttactttt aaaaagggaa catagaaatg tgtatcatta gagtagaaaa    13020

caatccttat tatcacaatt tgtcaaaaca agtttgttat taacacaagt agaatactgc    13080

attcaattaa gttgactgca gattttgtgt tttgttaaaa ttagaaagag ataacaacaa    13140

tttgaattat tgaaagtaac atgtaaatag ttctacatac gttcttttga catcttgttc    13200

aatcattgat cgaagttctt tatcttggaa gaatttgttc caaagactct gaaataagga    13260

aaacaatcta ttatatagtc tcacaccttt gttttacttt tagtgatttc aatttaataa    13320

tgtaaatggt taaaatttat tcttctctga gatcatttca cattgcagat agaaaacctg    13380

agactggggt aatttttatt aaaatctaat ttaatctcag aaacacatct ttattctaac    13440

atcaattttt ccagtttgat attatcatat aaagtcagcc ttcctcatct gcaggttcca    13500

caacaaaaat ccaaccaact gtggatcaaa aatattggga aaaaattaaa aatagcaata    13560

caacaataaa aaaatacaaa tcagaaaaac agcacagtat aacaacttta tttagcattt    13620

acaatctatt aggtattata agtaatctag                                     13650


<210> 10
<211> 22
<212> DNA
<213> Artificial Sequence

<223> "guide RNA AASV1"

<220> 
<223> guide RNA AASV1

<400> 10
ggggcgggcg gtgcgatgtc gt                                             22


<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence

<223> "5â primer"

<220> 
<223> 5â primer

<400> 11
agggccactt ctgctaatgg                                                20


<210> 12
<211> 19
<212> DNA
<213> Artificial Sequence

<223> "3â primer"

<220> 
<223> 3â primer

<400> 12
gataccgtcg gcgttggtg                                                 19


