                         SEQUENCE LISTING

<110>  Max-Planck-Gesellschaft zur Frderung der Wissenschaften 
       e.V.
 
<120>  Mutant light-inducible ion channels

<130>  30A-139 756

<150>  EP 16172992.6
<151>  2016-06-03

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  315
<212>  PRT
<213>  Chlamydomonas reinhardtii

<400>  1

Met Asp Tyr Gly Gly Ala Leu Ser Ala Val Gly Arg Glu Leu Leu Phe 
1               5                   10                  15      


Val Thr Asn Pro Val Val Val Asn Gly Ser Val Leu Val Pro Glu Asp 
            20                  25                  30          


Gln Cys Tyr Cys Ala Gly Trp Ile Glu Ser Arg Gly Thr Asn Gly Ala 
        35                  40                  45              


Gln Thr Ala Ser Asn Val Leu Gln Trp Leu Ala Ala Gly Phe Ser Ile 
    50                  55                  60                  


Leu Leu Leu Met Phe Tyr Ala Tyr Gln Thr Trp Lys Ser Thr Cys Gly 
65                  70                  75                  80  


Trp Glu Glu Ile Tyr Val Cys Ala Ile Glu Met Val Lys Val Ile Leu 
                85                  90                  95      


Glu Phe Phe Phe Glu Phe Lys Asn Pro Ser Met Leu Tyr Leu Ala Thr 
            100                 105                 110         


Gly His Arg Val Gln Trp Leu Arg Tyr Ala Glu Trp Leu Leu Thr Cys 
        115                 120                 125             


Pro Val Ile Leu Ile His Leu Ser Asn Leu Thr Gly Leu Ser Asn Asp 
    130                 135                 140                 


Tyr Ser Arg Arg Thr Met Gly Leu Leu Val Ser Asp Ile Gly Thr Ile 
145                 150                 155                 160 


Val Trp Gly Ala Thr Ser Ala Met Ala Thr Gly Tyr Val Lys Val Ile 
                165                 170                 175     


Phe Phe Cys Leu Gly Leu Cys Tyr Gly Ala Asn Thr Phe Phe His Ala 
            180                 185                 190         


Ala Lys Ala Tyr Ile Glu Gly Tyr His Thr Val Pro Lys Gly Arg Cys 
        195                 200                 205             


Arg Gln Val Val Thr Gly Met Ala Trp Leu Phe Phe Val Ser Trp Gly 
    210                 215                 220                 


Met Phe Pro Ile Leu Phe Ile Leu Gly Pro Glu Gly Phe Gly Val Leu 
225                 230                 235                 240 


Ser Val Tyr Gly Ser Thr Val Gly His Thr Ile Ile Asp Leu Met Ser 
                245                 250                 255     


Lys Asn Cys Trp Gly Leu Leu Gly His Tyr Leu Arg Val Leu Ile His 
            260                 265                 270         


Glu His Ile Leu Ile His Gly Asp Ile Arg Lys Thr Thr Lys Leu Asn 
        275                 280                 285             


Ile Gly Gly Thr Glu Ile Glu Val Glu Thr Leu Val Glu Asp Glu Ala 
    290                 295                 300                 


Glu Ala Gly Ala Val Asn Lys Gly Thr Gly Lys 
305                 310                 315 


<210>  2
<211>  300
<212>  PRT
<213>  Volvox carteri

<400>  2

Met Asp Tyr Pro Val Ala Arg Ser Leu Ile Val Arg Tyr Pro Thr Asp 
1               5                   10                  15      


Leu Gly Asn Gly Thr Val Cys Met Pro Arg Gly Gln Cys Tyr Cys Glu 
            20                  25                  30          


Gly Trp Leu Arg Ser Arg Gly Thr Ser Ile Glu Lys Thr Ile Ala Ile 
        35                  40                  45              


Thr Leu Gln Trp Val Val Phe Ala Leu Ser Val Ala Cys Leu Gly Trp 
    50                  55                  60                  


Tyr Ala Tyr Gln Ala Trp Arg Ala Thr Cys Gly Trp Glu Glu Val Tyr 
65                  70                  75                  80  


Val Ala Leu Ile Glu Met Met Lys Ser Ile Ile Glu Ala Phe His Glu 
                85                  90                  95      


Phe Asp Ser Pro Ala Thr Leu Trp Leu Ser Ser Gly Asn Gly Val Val 
            100                 105                 110         


Trp Met Arg Tyr Gly Glu Trp Leu Leu Thr Cys Pro Val Leu Leu Ile 
        115                 120                 125             


His Leu Ser Asn Leu Thr Gly Leu Lys Asp Asp Tyr Ser Lys Arg Thr 
    130                 135                 140                 


Met Gly Leu Leu Val Ser Asp Val Gly Cys Ile Val Trp Gly Ala Thr 
145                 150                 155                 160 


Ser Ala Met Cys Thr Gly Trp Thr Lys Ile Leu Phe Phe Leu Ile Ser 
                165                 170                 175     


Leu Ser Tyr Gly Met Tyr Thr Tyr Phe His Ala Ala Lys Val Tyr Ile 
            180                 185                 190         


Glu Ala Phe His Thr Val Pro Lys Gly Ile Cys Arg Glu Leu Val Arg 
        195                 200                 205             


Val Met Ala Trp Thr Phe Phe Val Ala Trp Gly Met Phe Pro Val Leu 
    210                 215                 220                 


Phe Leu Leu Gly Thr Glu Gly Phe Gly His Ile Ser Pro Tyr Gly Ser 
225                 230                 235                 240 


Ala Ile Gly His Ser Ile Leu Asp Leu Ile Ala Lys Asn Met Trp Gly 
                245                 250                 255     


Val Leu Gly Asn Tyr Leu Arg Val Lys Ile His Glu His Ile Leu Leu 
            260                 265                 270         


Tyr Gly Asp Ile Arg Lys Lys Gln Lys Ile Thr Ile Ala Gly Gln Glu 
        275                 280                 285             


Met Glu Val Glu Thr Leu Val Ala Glu Glu Glu Asp 
    290                 295                 300 


<210>  3
<211>  352
<212>  PRT
<213>  Artificial

<220>
<223>  ReaChR

<400>  3

Met Val Ser Arg Arg Pro Trp Leu Leu Ala Leu Ala Leu Ala Val Ala 
1               5                   10                  15      


Leu Ala Ala Gly Ser Ala Gly Ala Ser Thr Gly Ser Asp Ala Thr Val 
            20                  25                  30          


Pro Val Ala Thr Gln Asp Gly Pro Asp Tyr Val Phe His Arg Ala His 
        35                  40                  45              


Glu Arg Met Leu Phe Gln Thr Ser Tyr Thr Leu Glu Asn Asn Gly Ser 
    50                  55                  60                  


Val Ile Cys Ile Pro Asn Asn Gly Gln Cys Phe Cys Leu Ala Trp Leu 
65                  70                  75                  80  


Lys Ser Asn Gly Thr Asn Ala Glu Lys Leu Ala Ala Asn Ile Leu Gln 
                85                  90                  95      


Trp Val Val Phe Ala Leu Ser Val Ala Cys Leu Gly Trp Tyr Ala Tyr 
            100                 105                 110         


Gln Ala Trp Arg Ala Thr Cys Gly Trp Glu Glu Val Tyr Val Ala Leu 
        115                 120                 125             


Ile Glu Met Met Lys Ser Ile Ile Glu Ala Phe His Glu Phe Asp Ser 
    130                 135                 140                 


Pro Ala Thr Leu Trp Leu Ser Ser Gly Asn Gly Val Val Trp Met Arg 
145                 150                 155                 160 


Tyr Gly Glu Trp Leu Leu Thr Cys Pro Val Ile Leu Ile His Leu Ser 
                165                 170                 175     


Asn Leu Thr Gly Leu Lys Asp Asp Tyr Ser Lys Arg Thr Met Gly Leu 
            180                 185                 190         


Leu Val Ser Asp Val Gly Cys Ile Val Trp Gly Ala Thr Ser Ala Met 
        195                 200                 205             


Cys Thr Gly Trp Thr Lys Ile Leu Phe Phe Leu Ile Ser Leu Ser Tyr 
    210                 215                 220                 


Gly Met Tyr Thr Tyr Phe His Ala Ala Lys Val Tyr Ile Glu Ala Phe 
225                 230                 235                 240 


His Thr Val Pro Lys Gly Leu Cys Arg Gln Leu Val Arg Ala Met Ala 
                245                 250                 255     


Trp Leu Phe Phe Val Ser Trp Gly Met Phe Pro Val Leu Phe Leu Leu 
            260                 265                 270         


Gly Pro Glu Gly Phe Gly His Ile Ser Pro Tyr Gly Ser Ala Ile Gly 
        275                 280                 285             


His Ser Ile Leu Asp Leu Ile Ala Lys Asn Met Trp Gly Val Leu Gly 
    290                 295                 300                 


Asn Tyr Leu Arg Val Lys Ile His Glu His Ile Leu Leu Tyr Gly Asp 
305                 310                 315                 320 


Ile Arg Lys Lys Gln Lys Ile Thr Ile Ala Gly Gln Glu Met Glu Val 
                325                 330                 335     


Glu Thr Leu Val Ala Glu Glu Glu Asp Lys Tyr Glu Ser Ser Leu Glu 
            340                 345                 350         


<210>  4
<211>  25
<212>  PRT
<213>  Artificial

<220>
<223>  Helix 6 consensus motif


<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  wherein Xaa3 is Gln or Glu, in particular wherein Xaa3 is Gln

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  wherein Xaa4 is Val or Leu, in particular wherein Xaa4 is Val

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  wherein Xaa6 is Thr or Arg, in particular wherein Xaa6 is Thr

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  wherein Xaa7 is Gly, Val or Ala, in particular wherein Xaa7 is 
       Gly

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  wherein Xaa11 is Leu or Thr, in particular wherein Xaa11 is Leu

<220>
<221>  VARIANT
<222>  (15)..(15)
<223>  wherein Xaa15 is Ser or Ala, in particular wherein Xaa15 is Ser

<220>
<221>  VARIANT
<222>  (21)..(21)
<223>  wherein Xaa21 is Ile or Val, in particular wherein Xaa21 is Ile

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  wherein Xaa24 is Ile or Leu, in particular wherein Xaa24 is Ile

<400>  4

Cys Arg Xaa Xaa Val Xaa Xaa Met Ala Trp Xaa Tyr Phe Val Xaa Trp 
1               5                   10                  15      


Gly Met Phe Pro Xaa Leu Phe Xaa Leu 
            20                  25  


<210>  5
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Retinal binding site consensus motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  wherein Xaa1 is Leu, Ile, Ala or Cys

<220>
<221>  VARIANT
<222>  (3)..(5)
<223>  wherein Xaa3, Xaa4, and Xaa5 is independently any amino acid

<220>
<221>  VARIANT
<222>  (7)..(8)
<223>  wherein Xaa7 and Xaa8 is independently any amino acid

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  wherein Xaa9 is Thr, Phe, or Tyr

<400>  5

Xaa Asp Xaa Xaa Xaa Lys Xaa Xaa Xaa 
1               5                   


<210>  6
<211>  349
<212>  PRT
<213>  Artificial

<220>
<223>  Chrimson derived from Chlamydomonas noctigama

<400>  6

Met Ala Glu Leu Ile Ser Ser Ala Thr Arg Ser Leu Phe Ala Ala Gly 
1               5                   10                  15      


Gly Ile Asn Pro Trp Pro Asn Pro Tyr His His Glu Asp Met Gly Cys 
            20                  25                  30          


Gly Gly Met Thr Pro Thr Gly Glu Cys Phe Ser Thr Glu Trp Trp Cys 
        35                  40                  45              


Asp Pro Ser Tyr Gly Leu Ser Asp Ala Gly Tyr Gly Tyr Cys Phe Val 
    50                  55                  60                  


Glu Ala Thr Gly Gly Tyr Leu Val Val Gly Val Glu Lys Lys Gln Ala 
65                  70                  75                  80  


Trp Leu His Ser Arg Gly Thr Pro Gly Glu Lys Ile Gly Ala Gln Val 
                85                  90                  95      


Cys Gln Trp Ile Ala Phe Ser Ile Ala Ile Ala Leu Leu Thr Phe Tyr 
            100                 105                 110         


Gly Phe Ser Ala Trp Lys Ala Thr Cys Gly Trp Glu Glu Val Tyr Val 
        115                 120                 125             


Cys Cys Val Glu Val Leu Phe Val Thr Leu Glu Ile Phe Lys Glu Phe 
    130                 135                 140                 


Ser Ser Pro Ala Thr Val Tyr Leu Ser Thr Gly Asn His Ala Tyr Cys 
145                 150                 155                 160 


Leu Arg Tyr Phe Glu Trp Leu Leu Ser Cys Pro Val Ile Leu Ile Lys 
                165                 170                 175     


Leu Ser Asn Leu Ser Gly Leu Lys Asn Asp Tyr Ser Lys Arg Thr Met 
            180                 185                 190         


Gly Leu Ile Val Ser Cys Val Gly Met Ile Val Phe Gly Met Ala Ala 
        195                 200                 205             


Gly Leu Ala Thr Asp Trp Leu Lys Trp Leu Leu Tyr Ile Val Ser Cys 
    210                 215                 220                 


Ile Tyr Gly Gly Tyr Met Tyr Phe Gln Ala Ala Lys Cys Tyr Val Glu 
225                 230                 235                 240 


Ala Asn His Ser Val Pro Lys Gly His Cys Arg Met Val Val Lys Leu 
                245                 250                 255     


Met Ala Tyr Ala Tyr Phe Ala Ser Trp Gly Ser Tyr Pro Ile Leu Trp 
            260                 265                 270         


Ala Val Gly Pro Glu Gly Leu Leu Lys Leu Ser Pro Tyr Ala Asn Ser 
        275                 280                 285             


Ile Gly His Ser Ile Cys Asp Ile Ile Ala Lys Glu Phe Trp Thr Phe 
    290                 295                 300                 


Leu Ala His His Leu Arg Ile Lys Ile His Glu His Ile Leu Ile His 
305                 310                 315                 320 


Gly Asp Ile Arg Lys Thr Thr Lys Glu Ile Gly Gly Glu Glu Val Glu 
                325                 330                 335     


Val Glu Glu Phe Val Glu Glu Glu Asp Glu Asp Thr Val 
            340                 345                 


<210>  7
<211>  345
<212>  PRT
<213>  Artificial

<220>
<223>  CSChrimson

<400>  7

Met Ser Arg Leu Val Ala Ala Ser Trp Leu Leu Ala Leu Leu Leu Cys 
1               5                   10                  15      


Gly Ile Thr Ser Thr Thr Thr Ala Ser Ser Ala Pro Ala Ala Ser Ser 
            20                  25                  30          


Thr Asp Gly Thr Ala Ala Ala Ala Val Ser His Tyr Ala Met Asn Gly 
        35                  40                  45              


Phe Asp Glu Leu Ala Lys Gly Ala Val Val Pro Glu Asp His Phe Val 
    50                  55                  60                  


Cys Gly Pro Ala Asp Lys Cys Tyr Cys Ser Ala Trp Leu His Ser Arg 
65                  70                  75                  80  


Gly Thr Pro Gly Glu Lys Ile Gly Ala Gln Val Cys Gln Trp Ile Ala 
                85                  90                  95      


Phe Ser Ile Ala Ile Ala Leu Leu Thr Phe Tyr Gly Phe Ser Ala Trp 
            100                 105                 110         


Lys Ala Thr Cys Gly Trp Glu Glu Val Tyr Val Cys Cys Val Glu Val 
        115                 120                 125             


Leu Phe Val Thr Leu Glu Ile Phe Lys Glu Phe Ser Ser Pro Ala Thr 
    130                 135                 140                 


Val Tyr Leu Ser Thr Gly Asn His Ala Tyr Cys Leu Arg Tyr Phe Glu 
145                 150                 155                 160 


Trp Leu Leu Ser Cys Pro Val Ile Leu Ile Lys Leu Ser Asn Leu Ser 
                165                 170                 175     


Gly Leu Lys Asn Asp Tyr Ser Lys Arg Thr Met Gly Leu Ile Val Ser 
            180                 185                 190         


Cys Val Gly Met Ile Val Phe Gly Met Ala Ala Gly Leu Ala Thr Asp 
        195                 200                 205             


Trp Leu Lys Trp Leu Leu Tyr Ile Val Ser Cys Ile Tyr Gly Gly Tyr 
    210                 215                 220                 


Met Tyr Phe Gln Ala Ala Lys Cys Tyr Val Glu Ala Asn His Ser Val 
225                 230                 235                 240 


Pro Lys Gly His Cys Arg Met Val Val Lys Leu Met Ala Tyr Ala Tyr 
                245                 250                 255     


Phe Ala Ser Trp Gly Ser Tyr Pro Ile Leu Trp Ala Val Gly Pro Glu 
            260                 265                 270         


Gly Leu Leu Lys Leu Ser Pro Tyr Ala Asn Ser Ile Gly His Ser Ile 
        275                 280                 285             


Cys Asp Ile Ile Ala Lys Glu Phe Trp Thr Phe Leu Ala His His Leu 
    290                 295                 300                 


Arg Ile Lys Ile His Glu His Ile Leu Ile His Gly Asp Ile Arg Lys 
305                 310                 315                 320 


Thr Thr Lys Met Glu Ile Gly Gly Glu Glu Val Glu Val Glu Glu Phe 
                325                 330                 335     


Val Glu Glu Glu Asp Glu Asp Thr Val 
            340                 345 


<210>  8
<211>  348
<212>  PRT
<213>  Artificial

<220>
<223>  Chrimson Helix 6 Swap

<400>  8

Met Ala Glu Leu Ile Ser Ser Ala Thr Arg Ser Leu Phe Ala Ala Gly 
1               5                   10                  15      


Gly Ile Asn Pro Trp Pro Asn Pro Tyr His His Glu Asp Met Gly Cys 
            20                  25                  30          


Gly Gly Met Thr Pro Thr Gly Glu Cys Phe Ser Thr Glu Trp Trp Cys 
        35                  40                  45              


Asp Pro Ser Tyr Gly Leu Ser Asp Ala Gly Tyr Gly Tyr Cys Phe Val 
    50                  55                  60                  


Glu Ala Thr Gly Gly Tyr Leu Val Val Gly Val Glu Lys Lys Gln Ala 
65                  70                  75                  80  


Trp Leu His Ser Arg Gly Thr Pro Gly Glu Lys Ile Gly Ala Gln Val 
                85                  90                  95      


Cys Gln Trp Ile Ala Phe Ser Ile Ala Ile Ala Leu Leu Thr Phe Tyr 
            100                 105                 110         


Gly Phe Ser Ala Trp Lys Ala Thr Cys Gly Trp Glu Glu Val Tyr Val 
        115                 120                 125             


Cys Cys Val Glu Val Leu Phe Val Thr Leu Glu Ile Phe Lys Glu Phe 
    130                 135                 140                 


Ser Ser Pro Ala Thr Val Tyr Leu Ser Thr Gly Asn His Ala Tyr Cys 
145                 150                 155                 160 


Leu Arg Tyr Phe Glu Trp Leu Leu Ser Cys Pro Val Ile Leu Ile Lys 
                165                 170                 175     


Leu Ser Asn Leu Ser Gly Leu Lys Asn Asp Tyr Ser Lys Arg Thr Met 
            180                 185                 190         


Gly Leu Ile Val Ser Cys Val Gly Met Ile Val Phe Gly Met Ala Ala 
        195                 200                 205             


Gly Leu Ala Thr Asp Trp Leu Lys Trp Leu Leu Tyr Ile Val Ser Cys 
    210                 215                 220                 


Ile Tyr Gly Gly Tyr Met Tyr Phe Gln Ala Ala Lys Cys Tyr Val Glu 
225                 230                 235                 240 


Ala Asn His Ser Val Pro Lys Gly His Cys Arg Gln Val Val Thr Gly 
                245                 250                 255     


Met Ala Trp Leu Tyr Phe Val Ser Trp Gly Met Phe Pro Ile Leu Phe 
            260                 265                 270         


Ile Leu Pro Glu Gly Leu Leu Lys Leu Ser Pro Tyr Ala Asn Ser Ile 
        275                 280                 285             


Gly His Ser Ile Cys Asp Ile Ile Ala Lys Glu Phe Trp Thr Phe Leu 
    290                 295                 300                 


Ala His His Leu Arg Ile Lys Ile His Glu His Ile Leu Ile His Gly 
305                 310                 315                 320 


Asp Ile Arg Lys Thr Thr Lys Glu Ile Gly Gly Glu Glu Val Glu Val 
                325                 330                 335     


Glu Glu Phe Val Glu Glu Glu Asp Glu Asp Thr Val 
            340                 345             


<210>  9
<211>  344
<212>  PRT
<213>  Artificial

<220>
<223>  CSChrimson Helix 6 SWAP

<400>  9

Met Ser Arg Leu Val Ala Ala Ser Trp Leu Leu Ala Leu Leu Leu Cys 
1               5                   10                  15      


Gly Ile Thr Ser Thr Thr Thr Ala Ser Ser Ala Pro Ala Ala Ser Ser 
            20                  25                  30          


Thr Asp Gly Thr Ala Ala Ala Ala Val Ser His Tyr Ala Met Asn Gly 
        35                  40                  45              


Phe Asp Glu Leu Ala Lys Gly Ala Val Val Pro Glu Asp His Phe Val 
    50                  55                  60                  


Cys Gly Pro Ala Asp Lys Cys Tyr Cys Ser Ala Trp Leu His Ser Arg 
65                  70                  75                  80  


Gly Thr Pro Gly Glu Lys Ile Gly Ala Gln Val Cys Gln Trp Ile Ala 
                85                  90                  95      


Phe Ser Ile Ala Ile Ala Leu Leu Thr Phe Tyr Gly Phe Ser Ala Trp 
            100                 105                 110         


Lys Ala Thr Cys Gly Trp Glu Glu Val Tyr Val Cys Cys Val Glu Val 
        115                 120                 125             


Leu Phe Val Thr Leu Glu Ile Phe Lys Glu Phe Ser Ser Pro Ala Thr 
    130                 135                 140                 


Val Tyr Leu Ser Thr Gly Asn His Ala Tyr Cys Leu Arg Tyr Phe Glu 
145                 150                 155                 160 


Trp Leu Leu Ser Cys Pro Val Ile Leu Ile Lys Leu Ser Asn Leu Ser 
                165                 170                 175     


Gly Leu Lys Asn Asp Tyr Ser Lys Arg Thr Met Gly Leu Ile Val Ser 
            180                 185                 190         


Cys Val Gly Met Ile Val Phe Gly Met Ala Ala Gly Leu Ala Thr Asp 
        195                 200                 205             


Trp Leu Lys Trp Leu Leu Tyr Ile Val Ser Cys Ile Tyr Gly Gly Tyr 
    210                 215                 220                 


Met Tyr Phe Gln Ala Ala Lys Cys Tyr Val Glu Ala Asn His Ser Val 
225                 230                 235                 240 


Pro Lys Gly His Cys Arg Gln Val Val Thr Gly Met Ala Trp Leu Tyr 
                245                 250                 255     


Phe Val Ser Trp Gly Met Phe Pro Ile Leu Phe Ile Leu Pro Glu Gly 
            260                 265                 270         


Leu Leu Lys Leu Ser Pro Tyr Ala Asn Ser Ile Gly His Ser Ile Cys 
        275                 280                 285             


Asp Ile Ile Ala Lys Glu Phe Trp Thr Phe Leu Ala His His Leu Arg 
    290                 295                 300                 


Ile Lys Ile His Glu His Ile Leu Ile His Gly Asp Ile Arg Lys Thr 
305                 310                 315                 320 


Thr Lys Met Glu Ile Gly Gly Glu Glu Val Glu Val Glu Glu Phe Val 
                325                 330                 335     


Glu Glu Glu Asp Glu Asp Thr Val 
            340                 


