                         SEQUENCE LISTING

<110>  University of Zurich
 
<120>  Recombinant SIRT1

<130>  uz275wo

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  747
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Asp Glu Ala Ala Leu Ala Leu Gln Pro Gly Gly Ser Pro Ser 
1               5                   10                  15      


Ala Ala Gly Ala Asp Arg Glu Ala Ala Ser Ser Pro Ala Gly Glu Pro 
            20                  25                  30          


Leu Arg Lys Arg Pro Arg Arg Asp Gly Pro Gly Leu Glu Arg Ser Pro 
        35                  40                  45              


Gly Glu Pro Gly Gly Ala Ala Pro Glu Arg Glu Val Pro Ala Ala Ala 
    50                  55                  60                  


Arg Gly Cys Pro Gly Ala Ala Ala Ala Ala Leu Trp Arg Glu Ala Glu 
65                  70                  75                  80  


Ala Glu Ala Ala Ala Ala Gly Gly Glu Gln Glu Ala Gln Ala Thr Ala 
                85                  90                  95      


Ala Ala Gly Glu Gly Asp Asn Gly Pro Gly Leu Gln Gly Pro Ser Arg 
            100                 105                 110         


Glu Pro Pro Leu Ala Asp Asn Leu Tyr Asp Glu Asp Asp Asp Asp Glu 
        115                 120                 125             


Gly Glu Glu Glu Glu Glu Ala Ala Ala Ala Ala Ile Gly Tyr Arg Asp 
    130                 135                 140                 


Asn Leu Leu Phe Gly Asp Glu Ile Ile Thr Asn Gly Phe His Ser Cys 
145                 150                 155                 160 


Glu Ser Asp Glu Glu Asp Arg Ala Ser His Ala Ser Ser Ser Asp Trp 
                165                 170                 175     


Thr Pro Arg Pro Arg Ile Gly Pro Tyr Thr Phe Val Gln Gln His Leu 
            180                 185                 190         


Met Ile Gly Thr Asp Pro Arg Thr Ile Leu Lys Asp Leu Leu Pro Glu 
        195                 200                 205             


Thr Ile Pro Pro Pro Glu Leu Asp Asp Met Thr Leu Trp Gln Ile Val 
    210                 215                 220                 


Ile Asn Ile Leu Ser Glu Pro Pro Lys Arg Lys Lys Arg Lys Asp Ile 
225                 230                 235                 240 


Asn Thr Ile Glu Asp Ala Val Lys Leu Leu Gln Glu Cys Lys Lys Ile 
                245                 250                 255     


Ile Val Leu Thr Gly Ala Gly Val Ser Val Ser Cys Gly Ile Pro Asp 
            260                 265                 270         


Phe Arg Ser Arg Asp Gly Ile Tyr Ala Arg Leu Ala Val Asp Phe Pro 
        275                 280                 285             


Asp Leu Pro Asp Pro Gln Ala Met Phe Asp Ile Glu Tyr Phe Arg Lys 
    290                 295                 300                 


Asp Pro Arg Pro Phe Phe Lys Phe Ala Lys Glu Ile Tyr Pro Gly Gln 
305                 310                 315                 320 


Phe Gln Pro Ser Leu Cys His Lys Phe Ile Ala Leu Ser Asp Lys Glu 
                325                 330                 335     


Gly Lys Leu Leu Arg Asn Tyr Thr Gln Asn Ile Asp Thr Leu Glu Gln 
            340                 345                 350         


Val Ala Gly Ile Gln Arg Ile Ile Gln Cys His Gly Ser Phe Ala Thr 
        355                 360                 365             


Ala Ser Cys Leu Ile Cys Lys Tyr Lys Val Asp Cys Glu Ala Val Arg 
    370                 375                 380                 


Gly Asp Ile Phe Asn Gln Val Val Pro Arg Cys Pro Arg Cys Pro Ala 
385                 390                 395                 400 


Asp Glu Pro Leu Ala Ile Met Lys Pro Glu Ile Val Phe Phe Gly Glu 
                405                 410                 415     


Asn Leu Pro Glu Gln Phe His Arg Ala Met Lys Tyr Asp Lys Asp Glu 
            420                 425                 430         


Val Asp Leu Leu Ile Val Ile Gly Ser Ser Leu Lys Val Arg Pro Val 
        435                 440                 445             


Ala Leu Ile Pro Ser Ser Ile Pro His Glu Val Pro Gln Ile Leu Ile 
    450                 455                 460                 


Asn Arg Glu Pro Leu Pro His Leu His Phe Asp Val Glu Leu Leu Gly 
465                 470                 475                 480 


Asp Cys Asp Val Ile Ile Asn Glu Leu Cys His Arg Leu Gly Gly Glu 
                485                 490                 495     


Tyr Ala Lys Leu Cys Cys Asn Pro Val Lys Leu Ser Glu Ile Thr Glu 
            500                 505                 510         


Lys Pro Pro Arg Thr Gln Lys Glu Leu Ala Tyr Leu Ser Glu Leu Pro 
        515                 520                 525             


Pro Thr Pro Leu His Val Ser Glu Asp Ser Ser Ser Pro Glu Arg Thr 
    530                 535                 540                 


Ser Pro Pro Asp Ser Ser Val Ile Val Thr Leu Leu Asp Gln Ala Ala 
545                 550                 555                 560 


Lys Ser Asn Asp Asp Leu Asp Val Ser Glu Ser Lys Gly Cys Met Glu 
                565                 570                 575     


Glu Lys Pro Gln Glu Val Gln Thr Ser Arg Asn Val Glu Ser Ile Ala 
            580                 585                 590         


Glu Gln Met Glu Asn Pro Asp Leu Lys Asn Val Gly Ser Ser Thr Gly 
        595                 600                 605             


Glu Lys Asn Glu Arg Thr Ser Val Ala Gly Thr Val Arg Lys Cys Trp 
    610                 615                 620                 


Pro Asn Arg Val Ala Lys Glu Gln Ile Ser Arg Arg Leu Asp Gly Asn 
625                 630                 635                 640 


Gln Tyr Leu Phe Leu Pro Pro Asn Arg Tyr Ile Phe His Gly Ala Glu 
                645                 650                 655     


Val Tyr Ser Asp Ser Glu Asp Asp Val Leu Ser Ser Ser Ser Cys Gly 
            660                 665                 670         


Ser Asn Ser Asp Ser Gly Thr Cys Gln Ser Pro Ser Leu Glu Glu Pro 
        675                 680                 685             


Met Glu Asp Glu Ser Glu Ile Glu Glu Phe Tyr Asn Gly Leu Glu Asp 
    690                 695                 700                 


Glu Pro Asp Val Pro Glu Arg Ala Gly Gly Ala Gly Phe Gly Thr Asp 
705                 710                 715                 720 


Gly Asp Asp Gln Glu Ala Ile Asn Glu Ala Ile Ser Val Lys Gln Glu 
                725                 730                 735     


Val Thr Asp Met Asn Tyr Pro Ser Asn Lys Ser 
            740                 745         


<210>  2
<211>  561
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Asp Glu Ala Ala Leu Ala Leu Gln Pro Gly Gly Ser Pro Ser 
1               5                   10                  15      


Ala Ala Gly Ala Asp Arg Glu Ala Ala Ser Ser Pro Ala Gly Glu Pro 
            20                  25                  30          


Leu Arg Lys Arg Pro Arg Arg Asp Gly Pro Gly Leu Glu Arg Ser Pro 
        35                  40                  45              


Gly Glu Pro Gly Gly Ala Ala Pro Glu Arg Glu Val Pro Ala Ala Ala 
    50                  55                  60                  


Arg Gly Cys Pro Gly Ala Ala Ala Ala Ala Leu Trp Arg Glu Ala Glu 
65                  70                  75                  80  


Ala Glu Ala Ala Ala Ala Gly Gly Glu Gln Glu Ala Gln Ala Thr Ala 
                85                  90                  95      


Ala Ala Gly Glu Gly Asp Asn Gly Pro Gly Leu Gln Gly Pro Ser Arg 
            100                 105                 110         


Glu Pro Pro Leu Ala Asp Asn Leu Tyr Asp Glu Asp Asp Asp Asp Glu 
        115                 120                 125             


Gly Glu Glu Glu Glu Glu Ala Ala Ala Ala Ala Ile Gly Tyr Arg Asp 
    130                 135                 140                 


Asn Leu Leu Phe Gly Asp Glu Ile Ile Thr Asn Gly Phe His Ser Cys 
145                 150                 155                 160 


Glu Ser Asp Glu Glu Asp Arg Ala Ser His Ala Ser Ser Ser Asp Trp 
                165                 170                 175     


Thr Pro Arg Pro Arg Ile Gly Pro Tyr Thr Phe Val Gln Gln His Leu 
            180                 185                 190         


Met Ile Gly Thr Asp Pro Arg Thr Ile Leu Lys Asp Leu Leu Pro Glu 
        195                 200                 205             


Thr Ile Pro Pro Pro Glu Leu Asp Asp Met Thr Leu Trp Gln Ile Val 
    210                 215                 220                 


Ile Asn Ile Leu Ser Glu Pro Pro Lys Arg Lys Lys Arg Lys Asp Ile 
225                 230                 235                 240 


Asn Thr Ile Glu Asp Ala Val Lys Leu Leu Gln Glu Cys Lys Lys Ile 
                245                 250                 255     


Ile Val Leu Thr Gly Ala Gly Val Ser Val Ser Cys Gly Ile Pro Asp 
            260                 265                 270         


Phe Arg Ser Arg Asp Gly Ile Tyr Ala Arg Leu Ala Val Asp Phe Pro 
        275                 280                 285             


Asp Leu Pro Asp Pro Gln Ala Met Phe Asp Ile Glu Tyr Phe Arg Lys 
    290                 295                 300                 


Asp Pro Arg Pro Phe Phe Lys Phe Ala Lys Glu Ile Tyr Pro Gly Gln 
305                 310                 315                 320 


Phe Gln Pro Ser Leu Cys His Lys Phe Ile Ala Leu Ser Asp Lys Glu 
                325                 330                 335     


Gly Lys Leu Leu Arg Asn Tyr Thr Gln Asn Ile Asp Thr Leu Glu Gln 
            340                 345                 350         


Val Ala Gly Ile Gln Arg Ile Ile Gln Cys His Gly Ser Phe Ala Thr 
        355                 360                 365             


Ala Ser Cys Leu Ile Cys Lys Tyr Lys Val Asp Cys Glu Ala Val Arg 
    370                 375                 380                 


Gly Asp Ile Phe Asn Gln Val Val Pro Arg Cys Pro Arg Cys Pro Ala 
385                 390                 395                 400 


Asp Glu Pro Leu Ala Ile Met Lys Pro Glu Ile Val Phe Phe Gly Glu 
                405                 410                 415     


Asn Leu Pro Glu Gln Phe His Arg Ala Met Lys Tyr Asp Lys Asp Glu 
            420                 425                 430         


Val Asp Leu Leu Ile Val Ile Gly Ser Ser Leu Lys Val Arg Pro Val 
        435                 440                 445             


Ala Leu Ile Pro Ser Asn Gln Tyr Leu Phe Leu Pro Pro Asn Arg Tyr 
    450                 455                 460                 


Ile Phe His Gly Ala Glu Val Tyr Ser Asp Ser Glu Asp Asp Val Leu 
465                 470                 475                 480 


Ser Ser Ser Ser Cys Gly Ser Asn Ser Asp Ser Gly Thr Cys Gln Ser 
                485                 490                 495     


Pro Ser Leu Glu Glu Pro Met Glu Asp Glu Ser Glu Ile Glu Glu Phe 
            500                 505                 510         


Tyr Asn Gly Leu Glu Asp Glu Pro Asp Val Pro Glu Arg Ala Gly Gly 
        515                 520                 525             


Ala Gly Phe Gly Thr Asp Gly Asp Asp Gln Glu Ala Ile Asn Glu Ala 
    530                 535                 540                 


Ile Ser Val Lys Gln Glu Val Thr Asp Met Asn Tyr Pro Ser Asn Lys 
545                 550                 555                 560 


Ser 
    


<210>  3
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  3
tgggcggcat gatttcctc                                                    19


<210>  4
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  4
gccaggacat tgttgaccag                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  5
actgggccag gaatttgacg                                                   20


<210>  6
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  6
ctcgtggaag tgacgcctt                                                    19


<210>  7
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  7
aaaacggcct gaacctctcg                                                   20


<210>  8
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  8
acacagctca gcgttattct c                                                 21


<210>  9
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  9
gagccaccac tcaccctact                                                   20


<210>  10
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  10
ccaggcattc ggcaatgtg                                                    19


<210>  11
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  11
tgattgacct ttccagagca ag                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  12
ctaaaattgc cattccacga gc                                                22


<210>  13
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  13
catgtcttcc atggccttct                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  14
tagtcgacat ggggcaactt                                                   20


<210>  15
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  15
tctgcaacat ggctagagac t                                                 21


<210>  16
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  16
tccaagcatt cgttggtccc                                                   20


<210>  17
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  17
aaggacctgt ctaggtttga tgc                                               23


<210>  18
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  18
tggcttcata ggtgacttcc a                                                 21


<210>  19
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  19
tagccttgtc agataaggaa gga                                               23


<210>  20
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  20
acagcttcac agtcaacttt gt                                                22


<210>  21
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  21
gcctttgata taccagaatg cag                                               23


<210>  22
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  22
cctgctgctg aatggtgac                                                    19


<210>  23
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  23
cgctcttgtc gtgtctgttc a                                                 21


<210>  24
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  24
ccttcgcgta ctgacggaaa                                                   20


<210>  25
<211>  19
<212>  DNA
<213>  Mus musculus

<400>  25
gctgacgact tcgacgacg                                                    19


<210>  26
<211>  21
<212>  DNA
<213>  Mus musculus

<400>  26
tcggtcaaca ggaggttgtc t                                                 21


<210>  27
<211>  21
<212>  DNA
<213>  Mus musculus

<400>  27
gagacccaga ggctacagat t                                                 21


<210>  28
<211>  21
<212>  DNA
<213>  Mus musculus

<400>  28
aatgtactcc acatggggca a                                                 21


<210>  29
<211>  21
<212>  DNA
<213>  Mus musculus

<400>  29
tgactcagac gaacaaggct g                                                 21


<210>  30
<211>  21
<212>  DNA
<213>  Mus musculus

<400>  30
atctaggcaa tctcggtctc c                                                 21


<210>  31
<211>  20
<212>  DNA
<213>  Mus musculus

<400>  31
gtctgatccg caaatacggg                                                   20


<210>  32
<211>  21
<212>  DNA
<213>  Mus musculus

<400>  32
agcctatgtc cttcgcgtac t                                                 21


<210>  33
<211>  692
<212>  PRT
<213>  Homo sapiens

<400>  33

Met Gly Thr Val Ser Ser Arg Arg Ser Trp Trp Pro Leu Pro Leu Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Leu Leu Gly Pro Ala Gly Ala Arg Ala Gln Glu 
            20                  25                  30          


Asp Glu Asp Gly Asp Tyr Glu Glu Leu Val Leu Ala Leu Arg Ser Glu 
        35                  40                  45              


Glu Asp Gly Leu Ala Glu Ala Pro Glu His Gly Thr Thr Ala Thr Phe 
    50                  55                  60                  


His Arg Cys Ala Lys Asp Pro Trp Arg Leu Pro Gly Thr Tyr Val Val 
65                  70                  75                  80  


Val Leu Lys Glu Glu Thr His Leu Ser Gln Ser Glu Arg Thr Ala Arg 
                85                  90                  95      


Arg Leu Gln Ala Gln Ala Ala Arg Arg Gly Tyr Leu Thr Lys Ile Leu 
            100                 105                 110         


His Val Phe His Gly Leu Leu Pro Gly Phe Leu Val Lys Met Ser Gly 
        115                 120                 125             


Asp Leu Leu Glu Leu Ala Leu Lys Leu Pro His Val Asp Tyr Ile Glu 
    130                 135                 140                 


Glu Asp Ser Ser Val Phe Ala Gln Ser Ile Pro Trp Asn Leu Glu Arg 
145                 150                 155                 160 


Ile Thr Pro Pro Arg Tyr Arg Ala Asp Glu Tyr Gln Pro Pro Asp Gly 
                165                 170                 175     


Gly Ser Leu Val Glu Val Tyr Leu Leu Asp Thr Ser Ile Gln Ser Asp 
            180                 185                 190         


His Arg Glu Ile Glu Gly Arg Val Met Val Thr Asp Phe Glu Asn Val 
        195                 200                 205             


Pro Glu Glu Asp Gly Thr Arg Phe His Arg Gln Ala Ser Lys Cys Asp 
    210                 215                 220                 


Ser His Gly Thr His Leu Ala Gly Val Val Ser Gly Arg Asp Ala Gly 
225                 230                 235                 240 


Val Ala Lys Gly Ala Ser Met Arg Ser Leu Arg Val Leu Asn Cys Gln 
                245                 250                 255     


Gly Lys Gly Thr Val Ser Gly Thr Leu Ile Gly Leu Glu Phe Ile Arg 
            260                 265                 270         


Lys Ser Gln Leu Val Gln Pro Val Gly Pro Leu Val Val Leu Leu Pro 
        275                 280                 285             


Leu Ala Gly Gly Tyr Ser Arg Val Leu Asn Ala Ala Cys Gln Arg Leu 
    290                 295                 300                 


Ala Arg Ala Gly Val Val Leu Val Thr Ala Ala Gly Asn Phe Arg Asp 
305                 310                 315                 320 


Asp Ala Cys Leu Tyr Ser Pro Ala Ser Ala Pro Glu Val Ile Thr Val 
                325                 330                 335     


Gly Ala Thr Asn Ala Gln Asp Gln Pro Val Thr Leu Gly Thr Leu Gly 
            340                 345                 350         


Thr Asn Phe Gly Arg Cys Val Asp Leu Phe Ala Pro Gly Glu Asp Ile 
        355                 360                 365             


Ile Gly Ala Ser Ser Asp Cys Ser Thr Cys Phe Val Ser Gln Ser Gly 
    370                 375                 380                 


Thr Ser Gln Ala Ala Ala His Val Ala Gly Ile Ala Ala Met Met Leu 
385                 390                 395                 400 


Ser Ala Glu Pro Glu Leu Thr Leu Ala Glu Leu Arg Gln Arg Leu Ile 
                405                 410                 415     


His Phe Ser Ala Lys Asp Val Ile Asn Glu Ala Trp Phe Pro Glu Asp 
            420                 425                 430         


Gln Arg Val Leu Thr Pro Asn Leu Val Ala Ala Leu Pro Pro Ser Thr 
        435                 440                 445             


His Gly Ala Gly Trp Gln Leu Phe Cys Arg Thr Val Trp Ser Ala His 
    450                 455                 460                 


Ser Gly Pro Thr Arg Met Ala Thr Ala Val Ala Arg Cys Ala Pro Asp 
465                 470                 475                 480 


Glu Glu Leu Leu Ser Cys Ser Ser Phe Ser Arg Ser Gly Lys Arg Arg 
                485                 490                 495     


Gly Glu Arg Met Glu Ala Gln Gly Gly Lys Leu Val Cys Arg Ala His 
            500                 505                 510         


Asn Ala Phe Gly Gly Glu Gly Val Tyr Ala Ile Ala Arg Cys Cys Leu 
        515                 520                 525             


Leu Pro Gln Ala Asn Cys Ser Val His Thr Ala Pro Pro Ala Glu Ala 
    530                 535                 540                 


Ser Met Gly Thr Arg Val His Cys His Gln Gln Gly His Val Leu Thr 
545                 550                 555                 560 


Gly Cys Ser Ser His Trp Glu Val Glu Asp Leu Gly Thr His Lys Pro 
                565                 570                 575     


Pro Val Leu Arg Pro Arg Gly Gln Pro Asn Gln Cys Val Gly His Arg 
            580                 585                 590         


Glu Ala Ser Ile His Ala Ser Cys Cys His Ala Pro Gly Leu Glu Cys 
        595                 600                 605             


Lys Val Lys Glu His Gly Ile Pro Ala Pro Gln Glu Gln Val Thr Val 
    610                 615                 620                 


Ala Cys Glu Glu Gly Trp Thr Leu Thr Gly Cys Ser Ala Leu Pro Gly 
625                 630                 635                 640 


Thr Ser His Val Leu Gly Ala Tyr Ala Val Asp Asn Thr Cys Val Val 
                645                 650                 655     


Arg Ser Arg Asp Val Ser Thr Thr Gly Ser Thr Ser Glu Gly Ala Val 
            660                 665                 670         


Thr Ala Val Ala Ile Cys Cys Arg Ser Arg His Leu Ala Gln Ala Ser 
        675                 680                 685             


Gln Glu Leu Gln 
    690         


