
                                SEQUENCE LISTING

<110> Orphagen Pharmaceuticals, Inc.
      Thacher, Scott McNear
      Crowe, Paul Damian
      Tao, Haiyan
      Raheja, Raj Kumar

<120> THERAPEUTIC COMPOSITIONS CONTAINING RAR
  ALPHA ANTAGONISTS

<130> ORPH.002WO

<150> 62/338883           
<151> 2016-05-19  

<160> 14

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1395
<212> DNA
<213> Artificial Sequence

<220> 
<223> pGal4DBD_RARalpha LBD

<400> 1
atggattaca aggacgacgt catgaagcta ctgtcttcta tcgaacaagc atgcgatatt 60
tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag 120
aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat 180
ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct 240
cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta 300
acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca 360
gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg 420
gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccgggatc cgagtctgtg 480
agaaacgacc gaaacaagaa gaagaaggag gtgcccaagc ccgagtgctc tgagagctac 540
acgctgacgc cggaggtggg ggagctcatt gagaaggtgc gcaaagcgca ccaggaaacc 600
ttccctgccc tctgccagct gggcaaatac actacgaaca acagctcaga acaacgtgtc 660
tctctggaca ttgacctctg ggacaagttc agtgaactct ccaccaagtg catcattaag 720
actgtggagt tcgccaagca gctgcccggc ttcaccaccc tcaccatcgc cgaccagatc 780
accctcctca aggctgcctg cctggacatc ctgatcctgc ggatctgcac gcggtacacg 840
cccgagcagg acaccatgac cttctcggac gggctgaccc tgaaccggac ccagatgcac 900
aacgctggct tcggccccct caccgacctg gtctttgcct tcgccaacca gctgctgccc 960
ctggagatgg atgatgcgga gacggggctg ctcagcgcca tctgcctcat ctgcggagac 1020
cgccaggacc tggagcagcc ggaccgggtg gacatgctgc aggagccgct gctggaggcg 1080
ctaaaggtct acgtgcggaa gcggaggccc agccgccccc acatgttccc caagatgcta 1140
atgaagatta ctgacctgcg aagcatcagc gccaaggggg ctgagcgggt gatcacgctg 1200
aagatggaga tcccgggctc catgccgcct ctcatccagg aaatgttgga gaactcagag 1260
ggcctggaca ctctgagcgg acagccgggg ggtggggggc gggacggggg tggcctggcc 1320
cccccgccag gcagctgtag ccccagcctc agccccagct ccaacagaag cagcccggcc 1380
acccactccc cgtga                                                  1395

<210> 2
<211> 4576
<212> DNA
<213> Artificial Sequence

<220> 
<223> pFA-CMV vector

<400> 2
atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 60
gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 120
cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 180
acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 240
tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 300
ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 360
tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 420
acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 480
tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 540
gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccatcg 600
ataagcaagc ctcctgaatc atggattaca aggacgacgt catgaagcta ctgtcttcta 660
tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 720
agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 780
ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 840
tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 900
aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 960
tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 1020
taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 1080
cgccgggatc cgcccgggct ggaattctag aagcttctgc agagctcggt accagatctt 1140
gaataagtag atccaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg 1200
tgtattttag attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt 1260
catgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 1320
acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 1380
tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 1440
tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa 1500
ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 1560
ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 1620
ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 1680
tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 1740
caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 1800
gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 1860
aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 1920
ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc 1980
gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 2040
aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa 2100
ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 2160
aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 2220
cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 2280
cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 2340
ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 2400
gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag 2460
gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 2520
gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 2580
ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 2640
gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg 2700
ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 2760
gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 2820
tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 2880
accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 2940
aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 3000
aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 3060
atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 3120
cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 3180
aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 3240
ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 3300
ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 3360
gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 3420
catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat 3480
accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg 3540
gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc 3600
gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag 3660
ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc 3720
aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 3780
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 3840
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 3900
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 3960
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 4020
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 4080
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 4140
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 4200
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 4260
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 4320
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 4380
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 4440
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 4500
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 4560
taaccgtatt accgcc                                                 4576

<210> 3
<211> 307
<212> PRT
<213> Artificial Sequence

<220> 
<223> RARalpha LBD (aa156-462)

<400> 3
Glu Ser Val Arg Asn Asp Arg Asn Lys Lys Lys Lys Glu Val Pro Lys
 1               5                  10                  15      
Pro Glu Cys Ser Glu Ser Tyr Thr Leu Thr Pro Glu Val Gly Glu Leu
            20                  25                  30          
Ile Glu Lys Val Arg Lys Ala His Gln Glu Thr Phe Pro Ala Leu Cys
        35                  40                  45              
Gln Leu Gly Lys Tyr Thr Thr Asn Asn Ser Ser Glu Gln Arg Val Ser
    50                  55                  60                  
Leu Asp Ile Asp Leu Trp Asp Lys Phe Ser Glu Leu Ser Thr Lys Cys
65                  70                  75                  80  
Ile Ile Lys Thr Val Glu Phe Ala Lys Gln Leu Pro Gly Phe Thr Thr
                85                  90                  95      
Leu Thr Ile Ala Asp Gln Ile Thr Leu Leu Lys Ala Ala Cys Leu Asp
            100                 105                 110         
Ile Leu Ile Leu Arg Ile Cys Thr Arg Tyr Thr Pro Glu Gln Asp Thr
        115                 120                 125             
Met Thr Phe Ser Asp Gly Leu Thr Leu Asn Arg Thr Gln Met His Asn
    130                 135                 140                 
Ala Gly Phe Gly Pro Leu Thr Asp Leu Val Phe Ala Phe Ala Asn Gln
145                 150                 155                 160 
Leu Leu Pro Leu Glu Met Asp Asp Ala Glu Thr Gly Leu Leu Ser Ala
                165                 170                 175     
Ile Cys Leu Ile Cys Gly Asp Arg Gln Asp Leu Glu Gln Pro Asp Arg
            180                 185                 190         
Val Asp Met Leu Gln Glu Pro Leu Leu Glu Ala Leu Lys Val Tyr Val
        195                 200                 205             
Arg Lys Arg Arg Pro Ser Arg Pro His Met Phe Pro Lys Met Leu Met
    210                 215                 220                 
Lys Ile Thr Asp Leu Arg Ser Ile Ser Ala Lys Gly Ala Glu Arg Val
225                 230                 235                 240 
Ile Thr Leu Lys Met Glu Ile Pro Gly Ser Met Pro Pro Leu Ile Gln
                245                 250                 255     
Glu Met Leu Glu Asn Ser Glu Gly Leu Asp Thr Leu Ser Gly Gln Pro
            260                 265                 270         
Gly Gly Gly Gly Arg Asp Gly Gly Gly Leu Ala Pro Pro Pro Gly Ser
        275                 280                 285             
Cys Ser Pro Ser Leu Ser Pro Ser Ser Asn Arg Ser Ser Pro Ala Thr
    290                 295                 300                 
His Ser Pro
305         


<210> 4
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> human RARa FOR

<400> 4
atatggatcc gagtctgtga gaaacgaccg aaacaag                          37

<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> human RARa REV

<400> 5
tataaagctt agggctgtgt c                                           21

<210> 6
<211> 1374
<212> DNA
<213> Artificial Sequence

<220> 
<223> pGal4DBD_RARbeta LBD

<400> 6
atggattaca aggacgacgt catgaagcta ctgtcttcta tcgaacaagc atgcgatatt 60
tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag 120
aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat 180
ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct 240
cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta 300
acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca 360
gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg 420
gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccgggatc cgaatctgtc 480
aggaatgaca ggaacaagaa aaagaaggag acttcgaagc aagaatgcac agagagctat 540
gaaatgacag ctgagttgga cgatctcaca gagaagatcc gaaaagctca ccaggaaact 600
ttcccttcac tctgccagct gggtaaatac accacgaatt ccagtgctga ccatcgagtc 660
cgactggacc tgggcctctg ggacaaattc agtgaactgg ccaccaagtg cattattaag 720
atcgtggagt ttgctaaacg tctgcctggt ttcactggct tgaccatcgc agaccaaatt 780
accctgctga aggccgcctg cctggacatc ctgattctta gaatttgcac caggtatacc 840
ccagaacaag acaccatgac tttctcagac ggccttaccc taaatcgaac tcagatgcac 900
aatgctggat ttggtcctct gactgacctt gtgttcacct ttgccaacca gctcctgcct 960
ttggaaatgg atgacacaga aacaggcctt ctcagtgcca tctgcttaat ctgtggagac 1020
cgccaggacc ttgaggaacc gacaaaagta gataagctac aagaaccatt gctggaagca 1080
ctaaaaattt atatcagaaa aagacgaccc agcaagcctc acatgtttcc aaagatctta 1140
atgaaaatca cagatctccg tagcatcagt gctaaaggtg cagagcgtgt aattaccttg 1200
aaaatggaaa ttcctggatc aatgccacct ctcattcaag aaatgctgga gaattctgaa 1260
ggacatgaac ccttgacccc aagttcaagt gggaacacag cagagcacag tcctagcatc 1320
tcacccagct cagtggaaaa cagtggggtc agtcagtcac cactcgtgca ataa       1374

<210> 7
<211> 1365
<212> DNA
<213> Artificial Sequence

<220> 
<223> pGal4DBD_RARgamma LBD

<400> 7
atggattaca aggacgacgt catgaagcta ctgtcttcta tcgaacaagc atgcgatatt 60
tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag 120
aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat 180
ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct 240
cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta 300
acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca 360
gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg 420
gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccgggatc cgaagctgtg 480
cgaaatgacc ggaacaagaa gaagaaagag gtgaaggaag aagggtcacc tgacagctat 540
gagctgagcc ctcagttaga agagctcatc accaaggtca gcaaagccca tcaggagact 600
ttcccctcgc tctgccagct gggcaagtat accacgaact ccagtgcaga ccaccgcgtg 660
cagctggatc tggggctgtg ggacaagttc agtgagctgg ctaccaagtg catcatcaag 720
atcgtggagt ttgccaagcg gttgcctggc tttacagggc tcagcattgc tgaccagatc 780
actctgctca aagctgcctg cctagatatc ctgatgctgc gtatctgcac aaggtacacc 840
ccagagcagg acaccatgac cttctccgac gggctgaccc tgaaccggac ccagatgcac 900
aatgccggct tcgggcccct cacagacctt gtctttgcct ttgctgggca gctcctgccc 960
ctggagatgg atgacaccga gacagggctg ctcagcgcca tctgcctcat ctgcggagac 1020
cgcatggacc tggaggagcc cgaaaaagtg gacaagctgc aggagccact gctggaagcc 1080
ctgaggctgt acgcccggcg ccggcggccc agccagccct acatgttccc aaggatgcta 1140
atgaaaatca ccgacctccg gggcatcagc actaagggag ctgaaagggc cattactctg 1200
aagatggaga ttccaggccc gatgcctccc ttaatccgag agatgctgga gaaccctgaa 1260
atgtttgagg atgactcctc gcagcctggt ccccacccca atgcctctag cgaggatgag 1320
gttcctgggg gccagggcaa agggggcctg aagtccccag cctga                 1365

<210> 8
<211> 300
<212> PRT
<213> Artificial Sequence

<220> 
<223> RARbeta LBD (aa156-455 of human RARb transcript
      variant 3 (P10826-1))

<400> 8
Glu Ser Val Arg Asn Asp Arg Asn Lys Lys Lys Lys Glu Thr Ser Lys
 1               5                  10                  15      
Gln Glu Cys Thr Glu Ser Tyr Glu Met Thr Ala Glu Leu Asp Asp Leu
            20                  25                  30          
Thr Glu Lys Ile Arg Lys Ala His Gln Glu Thr Phe Pro Ser Leu Cys
        35                  40                  45              
Gln Leu Gly Lys Tyr Thr Thr Asn Ser Ser Ala Asp His Arg Val Arg
    50                  55                  60                  
Leu Asp Leu Gly Leu Trp Asp Lys Phe Ser Glu Leu Ala Thr Lys Cys
65                  70                  75                  80  
Ile Ile Lys Ile Val Glu Phe Ala Lys Arg Leu Pro Gly Phe Thr Gly
                85                  90                  95      
Leu Thr Ile Ala Asp Gln Ile Thr Leu Leu Lys Ala Ala Cys Leu Asp
            100                 105                 110         
Ile Leu Ile Leu Arg Ile Cys Thr Arg Tyr Thr Pro Glu Gln Asp Thr
        115                 120                 125             
Met Thr Phe Ser Asp Gly Leu Thr Leu Asn Arg Thr Gln Met His Asn
    130                 135                 140                 
Ala Gly Phe Gly Pro Leu Thr Asp Leu Val Phe Thr Phe Ala Asn Gln
145                 150                 155                 160 
Leu Leu Pro Leu Glu Met Asp Asp Thr Glu Thr Gly Leu Leu Ser Ala
                165                 170                 175     
Ile Cys Leu Ile Cys Gly Asp Arg Gln Asp Leu Glu Glu Pro Thr Lys
            180                 185                 190         
Val Asp Lys Leu Gln Glu Pro Leu Leu Glu Ala Leu Lys Ile Tyr Ile
        195                 200                 205             
Arg Lys Arg Arg Pro Ser Lys Pro His Met Phe Pro Lys Ile Leu Met
    210                 215                 220                 
Lys Ile Thr Asp Leu Arg Ser Ile Ser Ala Lys Gly Ala Glu Arg Val
225                 230                 235                 240 
Ile Thr Leu Lys Met Glu Ile Pro Gly Ser Met Pro Pro Leu Ile Gln
                245                 250                 255     
Glu Met Leu Glu Asn Ser Glu Gly His Glu Pro Leu Thr Pro Ser Ser
            260                 265                 270         
Ser Gly Asn Thr Ala Glu His Ser Pro Ser Ile Ser Pro Ser Ser Val
        275                 280                 285             
Glu Asn Ser Gly Val Ser Gln Ser Pro Leu Val Gln
    290                 295                 300 


<210> 9
<211> 297
<212> PRT
<213> Artificial Sequence

<220> 
<223> RARgamma LBD (aa158-454 of human RARg transcript
      variant 1)

<400> 9
Glu Ala Val Arg Asn Asp Arg Asn Lys Lys Lys Lys Glu Val Lys Glu
 1               5                  10                  15      
Glu Gly Ser Pro Asp Ser Tyr Glu Leu Ser Pro Gln Leu Glu Glu Leu
            20                  25                  30          
Ile Thr Lys Val Ser Lys Ala His Gln Glu Thr Phe Pro Ser Leu Cys
        35                  40                  45              
Gln Leu Gly Lys Tyr Thr Thr Asn Ser Ser Ala Asp His Arg Val Gln
    50                  55                  60                  
Leu Asp Leu Gly Leu Trp Asp Lys Phe Ser Glu Leu Ala Thr Lys Cys
65                  70                  75                  80  
Ile Ile Lys Ile Val Glu Phe Ala Lys Arg Leu Pro Gly Phe Thr Gly
                85                  90                  95      
Leu Ser Ile Ala Asp Gln Ile Thr Leu Leu Lys Ala Ala Cys Leu Asp
            100                 105                 110         
Ile Leu Met Leu Arg Ile Cys Thr Arg Tyr Thr Pro Glu Gln Asp Thr
        115                 120                 125             
Met Thr Phe Ser Asp Gly Leu Thr Leu Asn Arg Thr Gln Met His Asn
    130                 135                 140                 
Ala Gly Phe Gly Pro Leu Thr Asp Leu Val Phe Ala Phe Ala Gly Gln
145                 150                 155                 160 
Leu Leu Pro Leu Glu Met Asp Asp Thr Glu Thr Gly Leu Leu Ser Ala
                165                 170                 175     
Ile Cys Leu Ile Cys Gly Asp Arg Met Asp Leu Glu Glu Pro Glu Lys
            180                 185                 190         
Val Asp Lys Leu Gln Glu Pro Leu Leu Glu Ala Leu Arg Leu Tyr Ala
        195                 200                 205             
Arg Arg Arg Arg Pro Ser Gln Pro Tyr Met Phe Pro Arg Met Leu Met
    210                 215                 220                 
Lys Ile Thr Asp Leu Arg Gly Ile Ser Thr Lys Gly Ala Glu Arg Ala
225                 230                 235                 240 
Ile Thr Leu Lys Met Glu Ile Pro Gly Pro Met Pro Pro Leu Ile Arg
                245                 250                 255     
Glu Met Leu Glu Asn Pro Glu Met Phe Glu Asp Asp Ser Ser Gln Pro
            260                 265                 270         
Gly Pro His Pro Asn Ala Ser Ser Glu Asp Glu Val Pro Gly Gly Gln
        275                 280                 285             
Gly Lys Gly Gly Leu Lys Ser Pro Ala
    290                 295         


<210> 10
<211> 1404
<212> DNA
<213> Artificial Sequence

<220> 
<223> pGal4DBD_PPARgamma LBD

<400> 10
atggattaca aggacgacgt catgaagcta ctgtcttcta tcgaacaagc atgcgatatt 60
tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag 120
aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat 180
ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct 240
cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta 300
acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca 360
gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg 420
gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccgggatc cgcccgggct 480
ggaattctag agatgtctca taatgccatc aggtttgggc ggatgccaca ggccgagaag 540
gagaagctgt tggcggagat ctccagtgat atcgaccagc tgaatccaga gtccgctgac 600
ctccgggccc tggcaaaaca tttgtatgac tcatacataa agtccttccc gctgaccaaa 660
gcaaaggcga gggcgatctt gacaggaaag acaacagaca aatcaccatt cgttatctat 720
gacatgaatt ccttaatgat gggagaagat aaaatcaagt tcaaacacat cacccccctg 780
caggagcaga gcaaagaggt ggccatccgc atctttcagg gctgccagtt tcgctccgtg 840
gaggctgtgc aggagatcac agagtatgcc aaaagcattc ctggttttgt aaatcttgac 900
ttgaacgacc aagtaactct cctcaaatat ggagtccacg agatcattta cacaatgctg 960
gcctccttga tgaataaaga tggggttctc atatccgagg gccaaggctt catgacaagg 1020
gagtttctaa agagcctgcg aaagcctttt ggtgacttta tggagcccaa gtttgagttt 1080
gctgtgaagt tcaatgcact ggaattagat gacagcgact tggcaatatt tattgctgtc 1140
attattctca gtggagaccg cccaggtttg ctgaatgtga agcccattga agacattcaa 1200
gacaacctgc tacaagccct ggagctccag ctgaagctga accaccctga gtcctcacag 1260
ctgtttgcca agctgctcca gaaaatgaca gacctcagac agattgtcac ggaacacgtg 1320
cagctactgc aggtgatcaa gaagacggag acagacatga gtcttcaccc gctcctgcag 1380
gagatctaca aggacttgta ctag                                        1404

<210> 11
<211> 303
<212> PRT
<213> Artificial Sequence

<220> 
<223> PPARgamma LBD

<400> 11
Met Ser His Asn Ala Ile Arg Phe Gly Arg Met Pro Gln Ala Glu Lys
 1               5                  10                  15      
Glu Lys Leu Leu Ala Glu Ile Ser Ser Asp Ile Asp Gln Leu Asn Pro
            20                  25                  30          
Glu Ser Ala Asp Leu Arg Ala Leu Ala Lys His Leu Tyr Asp Ser Tyr
        35                  40                  45              
Ile Lys Ser Phe Pro Leu Thr Lys Ala Lys Ala Arg Ala Ile Leu Thr
    50                  55                  60                  
Gly Lys Thr Thr Asp Lys Ser Pro Phe Val Ile Tyr Asp Met Asn Ser
65                  70                  75                  80  
Leu Met Met Gly Glu Asp Lys Ile Lys Phe Lys His Ile Thr Pro Leu
                85                  90                  95      
Gln Glu Gln Ser Lys Glu Val Ala Ile Arg Ile Phe Gln Gly Cys Gln
            100                 105                 110         
Phe Arg Ser Val Glu Ala Val Gln Glu Ile Thr Glu Tyr Ala Lys Ser
        115                 120                 125             
Ile Pro Gly Phe Val Asn Leu Asp Leu Asn Asp Gln Val Thr Leu Leu
    130                 135                 140                 
Lys Tyr Gly Val His Glu Ile Ile Tyr Thr Met Leu Ala Ser Leu Met
145                 150                 155                 160 
Asn Lys Asp Gly Val Leu Ile Ser Glu Gly Gln Gly Phe Met Thr Arg
                165                 170                 175     
Glu Phe Leu Lys Ser Leu Arg Lys Pro Phe Gly Asp Phe Met Glu Pro
            180                 185                 190         
Lys Phe Glu Phe Ala Val Lys Phe Asn Ala Leu Glu Leu Asp Asp Ser
        195                 200                 205             
Asp Leu Ala Ile Phe Ile Ala Val Ile Ile Leu Ser Gly Asp Arg Pro
    210                 215                 220                 
Gly Leu Leu Asn Val Lys Pro Ile Glu Asp Ile Gln Asp Asn Leu Leu
225                 230                 235                 240 
Gln Ala Leu Glu Leu Gln Leu Lys Leu Asn His Pro Glu Ser Ser Gln
                245                 250                 255     
Leu Phe Ala Lys Leu Leu Gln Lys Met Thr Asp Leu Arg Gln Ile Val
            260                 265                 270         
Thr Glu His Val Gln Leu Leu Gln Val Ile Lys Lys Thr Glu Thr Asp
        275                 280                 285             
Met Ser Leu His Pro Leu Leu Gln Glu Ile Tyr Lys Asp Leu Tyr
    290                 295                 300             


<210> 12
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> PPARgamma FOR

<400> 12
tatatctaga gatgtctcat aatgccatca g                                31

<210> 13
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> PPARgamma REV

<400> 13
tataggtacc ctctgctagt acaagtcctt g                                31

<210> 14
<211> 4955
<212> DNA
<213> Artificial Sequence

<220> 
<223> pG5luc

<400> 14
ggtaccgagt ttctagacgg agtactgtcc tccgagcgga gtactgtcct ccgactcgag 60
cggagtactg tcctccgatc ggagtactgt cctccgcgaa ttccggagta ctgtcctccg 120
aagacgctag cggggggcta taaaaggggg tgggggcgtt cgtcctcact ctagatctgc 180
gatctaagta agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa 240
acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac 300
tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac 360
atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag 420
ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc 480
ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga 540
acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg 600
tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca 660
tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca 720
cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct 780
tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta 840
aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta 900
tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg 960
gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt 1020
atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc 1080
tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt 1140
tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag 1200
cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta 1260
catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg 1320
ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc 1380
aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg 1440
aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact 1500
gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag 1560
gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg 1620
acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg 1680
ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag 1740
taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc 1800
ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg 1860
gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc gcttcgagca gacatgataa 1920
gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 1980
gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 2040
acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 2100
aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc 2160
ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 2220
gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc 2280
cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 2340
tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 2400
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 2460
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 2520
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 2580
tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 2640
ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 2700
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 2760
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 2820
caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 2880
ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 2940
cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 3000
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 3060
cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 3120
gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 3180
aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 3240
acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 3300
gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 3360
cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 3420
cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 3480
tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 3540
cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 3600
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 3660
cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 3720
ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 3780
gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 3840
taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 3900
gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 3960
acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 4020
aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 4080
cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 4140
atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 4200
gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4260
cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 4320
tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 4380
ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 4440
tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4500
taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 4560
tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 4620
aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt cgccattcag 4680
gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa 4740
gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg ccgcaataaa 4800
atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca 4860
tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt 4920
gcaagtgcag gtgccagaac atttctctat cgata                            4955
