               SEQUENCE LISTING

<110> Chr. Hansen A/S

<120> Variants of chymosin with improved milk-clotting properties

<130> P6081PC00

<160> 5

<170> BiSSAP 1.3.6

<210> 1
<211> 969
<212> DNA
<213> Camelus


<400> 1
gggaaggtgg ccagggaacc cctgaccagc tacctggata gtcagtactt tgggaagatc      60

tacatcggga ccccacccca ggagttcacc gtggtgtttg acactggctc ctctgacctg     120

tgggtgccct ctatctactg caagagcaat gtctgcaaaa accaccaccg ctttgacccg     180

agaaagtcgt ccaccttccg gaacctgggc aagcccctgt ccatccatta cggcacgggc     240

agcatggagg gctttctggg ctacgacacc gtcaccgtct ccaacattgt ggaccccaac     300

cagactgtgg gcctgagcac cgagcaacct ggcgaggtct tcacctactc cgagtttgac     360

gggatcctgg ggctggccta cccctcgctt gcctccgagt actcggtgcc cgtgtttgac     420

aatatgatgg acagacacct ggtggcccga gacctgttct cggtttacat ggacaggaat     480

ggccagggga gcatgcttac actgggggcc attgacccgt cctactacac cggctccctg     540

cactgggtgc ccgtgacctt gcagcagtac tggcagttca ccgtggacag tgtcaccatc     600

aacggggtgg cagtggcctg tgttggtggc tgtcaggcca tcctggacac gggtacctcc     660

gtgctgttcg ggcccagcag cgacatcctc aaaattcaga tggctattgg agccacagag     720

aaccgatatg gtgagtttga cgtcaactgt gggaacctga ggagcatgcc caccgtggtc     780

ttcgagatca atggcagaga ctacccactg tccccctccg cctacacaag caaggaccag     840

ggcttctgca ccagtggctt tcaaggtgac aacaattccg agctgtggat cctgggggat     900

gtcttcatcc gggagtatta cagtgtcttt gacagggcca acaatcgcgt ggggctggcc     960

aaggccatc                                                             969


<210> 2
<211> 323
<212> PRT
<213> Camelus


<400> 2
Gly Lys Val Ala Arg Glu Pro Leu Thr Ser Tyr Leu Asp Ser Gln Tyr 
1               5                   10                  15      
Phe Gly Lys Ile Tyr Ile Gly Thr Pro Pro Gln Glu Phe Thr Val Val 
            20                  25                  30          
Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Pro Ser Ile Tyr Cys Lys 
        35                  40                  45              
Ser Asn Val Cys Lys Asn His His Arg Phe Asp Pro Arg Lys Ser Ser 
    50                  55                  60                  
Thr Phe Arg Asn Leu Gly Lys Pro Leu Ser Ile His Tyr Gly Thr Gly 
65                  70                  75                  80  
Ser Met Glu Gly Phe Leu Gly Tyr Asp Thr Val Thr Val Ser Asn Ile 
                85                  90                  95      
Val Asp Pro Asn Gln Thr Val Gly Leu Ser Thr Glu Gln Pro Gly Glu 
            100                 105                 110         
Val Phe Thr Tyr Ser Glu Phe Asp Gly Ile Leu Gly Leu Ala Tyr Pro 
        115                 120                 125             
Ser Leu Ala Ser Glu Tyr Ser Val Pro Val Phe Asp Asn Met Met Asp 
    130                 135                 140                 
Arg His Leu Val Ala Arg Asp Leu Phe Ser Val Tyr Met Asp Arg Asn 
145                 150                 155                 160 
Gly Gln Gly Ser Met Leu Thr Leu Gly Ala Ile Asp Pro Ser Tyr Tyr 
                165                 170                 175     
Thr Gly Ser Leu His Trp Val Pro Val Thr Leu Gln Gln Tyr Trp Gln 
            180                 185                 190         
Phe Thr Val Asp Ser Val Thr Ile Asn Gly Val Ala Val Ala Cys Val 
        195                 200                 205             
Gly Gly Cys Gln Ala Ile Leu Asp Thr Gly Thr Ser Val Leu Phe Gly 
    210                 215                 220                 
Pro Ser Ser Asp Ile Leu Lys Ile Gln Met Ala Ile Gly Ala Thr Glu 
225                 230                 235                 240 
Asn Arg Tyr Gly Glu Phe Asp Val Asn Cys Gly Asn Leu Arg Ser Met 
                245                 250                 255     
Pro Thr Val Val Phe Glu Ile Asn Gly Arg Asp Tyr Pro Leu Ser Pro 
            260                 265                 270         
Ser Ala Tyr Thr Ser Lys Asp Gln Gly Phe Cys Thr Ser Gly Phe Gln 
        275                 280                 285             
Gly Asp Asn Asn Ser Glu Leu Trp Ile Leu Gly Asp Val Phe Ile Arg 
    290                 295                 300                 
Glu Tyr Tyr Ser Val Phe Asp Arg Ala Asn Asn Arg Val Gly Leu Ala 
305                 310                 315                 320 
Lys Ala Ile 
            

<210> 3
<211> 323
<212> PRT
<213> Bos


<400> 3
Gly Glu Val Ala Ser Val Pro Leu Thr Asn Tyr Leu Asp Ser Gln Tyr 
1               5                   10                  15      
Phe Gly Lys Ile Tyr Leu Gly Thr Pro Pro Gln Glu Phe Thr Val Leu 
            20                  25                  30          
Phe Asp Thr Gly Ser Ser Asp Phe Trp Val Pro Ser Ile Tyr Cys Lys 
        35                  40                  45              
Ser Asn Ala Cys Lys Asn His Gln Arg Phe Asp Pro Arg Lys Ser Ser 
    50                  55                  60                  
Thr Phe Gln Asn Leu Gly Lys Pro Leu Ser Ile His Tyr Gly Thr Gly 
65                  70                  75                  80  
Ser Met Gln Gly Ile Leu Gly Tyr Asp Thr Val Thr Val Ser Asn Ile 
                85                  90                  95      
Val Asp Ile Gln Gln Thr Val Gly Leu Ser Thr Gln Glu Pro Gly Asp 
            100                 105                 110         
Val Phe Thr Tyr Ala Glu Phe Asp Gly Ile Leu Gly Met Ala Tyr Pro 
        115                 120                 125             
Ser Leu Ala Ser Glu Tyr Ser Ile Pro Val Phe Asp Asn Met Met Asn 
    130                 135                 140                 
Arg His Leu Val Ala Gln Asp Leu Phe Ser Val Tyr Met Asp Arg Asn 
145                 150                 155                 160 
Gly Gln Glu Ser Met Leu Thr Leu Gly Ala Ile Asp Pro Ser Tyr Tyr 
                165                 170                 175     
Thr Gly Ser Leu His Trp Val Pro Val Thr Val Gln Gln Tyr Trp Gln 
            180                 185                 190         
Phe Thr Val Asp Ser Val Thr Ile Ser Gly Val Val Val Ala Cys Glu 
        195                 200                 205             
Gly Gly Cys Gln Ala Ile Leu Asp Thr Gly Thr Ser Lys Leu Val Gly 
    210                 215                 220                 
Pro Ser Ser Asp Ile Leu Asn Ile Gln Gln Ala Ile Gly Ala Thr Gln 
225                 230                 235                 240 
Asn Gln Tyr Gly Glu Phe Asp Ile Asp Cys Asp Asn Leu Ser Tyr Met 
                245                 250                 255     
Pro Thr Val Val Phe Glu Ile Asn Gly Lys Met Tyr Pro Leu Thr Pro 
            260                 265                 270         
Ser Ala Tyr Thr Ser Gln Asp Gln Gly Phe Cys Thr Ser Gly Phe Gln 
        275                 280                 285             
Ser Glu Asn His Ser Gln Lys Trp Ile Leu Gly Asp Val Phe Ile Arg 
    290                 295                 300                 
Glu Tyr Tyr Ser Val Phe Asp Arg Ala Asn Asn Leu Val Gly Leu Ala 
305                 310                 315                 320 
Lys Ala Ile 
            

<210> 4
<211> 1095
<212> DNA
<213> Camelus


<400> 4
agtgggatca ccaggatccc tctgcacaaa ggcaagactc tgagaaaagc gctgaaggag      60

cgtgggctcc tggaggactt tctgcagaga caacagtatg ccgtcagcag caagtactcc     120

agcttgggga aggtggccag ggaacccctg accagctacc tggatagtca gtactttggg     180

aagatctaca tcgggacccc accccaggag ttcaccgtgg tgtttgacac tggctcctct     240

gacctgtggg tgccctctat ctactgcaag agcaatgtct gcaaaaacca ccaccgcttt     300

gacccgagaa agtcgtccac cttccggaac ctgggcaagc ccctgtccat ccattacggc     360

acgggcagca tggagggctt tctgggctac gacaccgtca ccgtctccaa cattgtggac     420

cccaaccaga ctgtgggcct gagcaccgag caacctggcg aggtcttcac ctactccgag     480

tttgacggga tcctggggct ggcctacccc tcgcttgcct ccgagtactc ggtgcccgtg     540

tttgacaata tgatggacag acacctggtg gcccgagacc tgttctcggt ttacatggac     600

aggaatggcc aggggagcat gcttacactg ggggccattg acccgtccta ctacaccggc     660

tccctgcact gggtgcccgt gaccttgcag cagtactggc agttcaccgt ggacagtgtc     720

accatcaacg gggtggcagt ggcctgtgtt ggtggctgtc aggccatcct ggacacgggt     780

acctccgtgc tgttcgggcc cagcagcgac atcctcaaaa ttcagatggc tattggagcc     840

acagagaacc gatatggtga gtttgacgtc aactgtggga acctgaggag catgcccacc     900

gtggtcttcg agatcaatgg cagagactac ccactgtccc cctccgccta cacaagcaag     960

gaccagggct tctgcaccag tggctttcaa ggtgacaaca attccgagct gtggatcctg    1020

ggggatgtct tcatccggga gtattacagt gtctttgaca gggccaacaa tcgcgtgggg    1080

ctggccaagg ccatc                                                     1095


<210> 5
<211> 365
<212> PRT
<213> Camelus


<400> 5
Ser Gly Ile Thr Arg Ile Pro Leu His Lys Gly Lys Thr Leu Arg Lys 
1               5                   10                  15      
Ala Leu Lys Glu Arg Gly Leu Leu Glu Asp Phe Leu Gln Arg Gln Gln 
            20                  25                  30          
Tyr Ala Val Ser Ser Lys Tyr Ser Ser Leu Gly Lys Val Ala Arg Glu 
        35                  40                  45              
Pro Leu Thr Ser Tyr Leu Asp Ser Gln Tyr Phe Gly Lys Ile Tyr Ile 
    50                  55                  60                  
Gly Thr Pro Pro Gln Glu Phe Thr Val Val Phe Asp Thr Gly Ser Ser 
65                  70                  75                  80  
Asp Leu Trp Val Pro Ser Ile Tyr Cys Lys Ser Asn Val Cys Lys Asn 
                85                  90                  95      
His His Arg Phe Asp Pro Arg Lys Ser Ser Thr Phe Arg Asn Leu Gly 
            100                 105                 110         
Lys Pro Leu Ser Ile His Tyr Gly Thr Gly Ser Met Glu Gly Phe Leu 
        115                 120                 125             
Gly Tyr Asp Thr Val Thr Val Ser Asn Ile Val Asp Pro Asn Gln Thr 
    130                 135                 140                 
Val Gly Leu Ser Thr Glu Gln Pro Gly Glu Val Phe Thr Tyr Ser Glu 
145                 150                 155                 160 
Phe Asp Gly Ile Leu Gly Leu Ala Tyr Pro Ser Leu Ala Ser Glu Tyr 
                165                 170                 175     
Ser Val Pro Val Phe Asp Asn Met Met Asp Arg His Leu Val Ala Arg 
            180                 185                 190         
Asp Leu Phe Ser Val Tyr Met Asp Arg Asn Gly Gln Gly Ser Met Leu 
        195                 200                 205             
Thr Leu Gly Ala Ile Asp Pro Ser Tyr Tyr Thr Gly Ser Leu His Trp 
    210                 215                 220                 
Val Pro Val Thr Leu Gln Gln Tyr Trp Gln Phe Thr Val Asp Ser Val 
225                 230                 235                 240 
Thr Ile Asn Gly Val Ala Val Ala Cys Val Gly Gly Cys Gln Ala Ile 
                245                 250                 255     
Leu Asp Thr Gly Thr Ser Val Leu Phe Gly Pro Ser Ser Asp Ile Leu 
            260                 265                 270         
Lys Ile Gln Met Ala Ile Gly Ala Thr Glu Asn Arg Tyr Gly Glu Phe 
        275                 280                 285             
Asp Val Asn Cys Gly Asn Leu Arg Ser Met Pro Thr Val Val Phe Glu 
    290                 295                 300                 
Ile Asn Gly Arg Asp Tyr Pro Leu Ser Pro Ser Ala Tyr Thr Ser Lys 
305                 310                 315                 320 
Asp Gln Gly Phe Cys Thr Ser Gly Phe Gln Gly Asp Asn Asn Ser Glu 
                325                 330                 335     
Leu Trp Ile Leu Gly Asp Val Phe Ile Arg Glu Tyr Tyr Ser Val Phe 
            340                 345                 350         
Asp Arg Ala Asn Asn Arg Val Gly Leu Ala Lys Ala Ile 
        355                 360                 365 

