                         SEQUENCE LISTING

<110>  THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE
       CHATTERJEE, ANUSHREE
       OTOUPAL, PETER
 
<120>  COMPOSITIONS AND METHODS FOR ALTERING BACTERIAL FITNESS

<130>  466888.54

<150>  62334967
<151>  2016-05-11

<160>  108   

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  1
ctgctgcatc atgggcgtat                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  2
ctacatcaat gttaagcggc                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E.coli

<400>  3
ggctcaggcc gataagaatg                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  4
agcatcagaa cgaccgccag                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  5
taccaaattg tttctcaatc                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  6
attgtcgcgc atctccactg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  7
ccagtccaaa atgctatgaa                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  8
gcaagtacgc aaaattgtat                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  9
gcgtttcgcc acttcgccgg                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  10
agcagtcatg tgttaaattg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  11
gagccacatc gaggatgtgt                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  12
ccgtgatgcg gtgcgtcgtc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  13
gcaaaagctg gataagcagc                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  14
gttttgttca atgcgatgca                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  15
gtgataaaat cgccaacttc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  16
ctggcaacga gttaccgata                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  17
gtatacttgt aattttctta                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  18
agccctgatt aaacatatta                                                   20


<210>  19
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  19
actagtacta gtgcaagtac gcaaaattgt atgttttaga gctagaaata gc               52


<210>  20
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  20
actagtacta gtctgctgca tcatgggcgt atgttttaga gctagaaata gc               52


<210>  21
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  21
actagtacta gtgcgtttcg ccacttcgcc gggttttaga gctagaaata gc               52


<210>  22
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  22
actagtacta gtctacatca atgttaagcg gcgttttaga gctagaaata gc               52


<210>  23
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  23
actagtacta gtagcagtca tgtgttaaat tggttttaga gctagaaata gc               52


<210>  24
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  24
actagtacta gtggctcagg ccgataagaa tggttttaga gctagaaata gc               52


<210>  25
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  25
actagtacta gtgagccaca tcgaggatgt gtgttttaga gctagaaata gc               52


<210>  26
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  26
actagtacta gtagcatcag aacgaccgcc aggttttaga gctagaaata gc               52


<210>  27
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  27
actagtacta gtccgtgatg cggtgcgtcg tcgttttaga gctagaaata gc               52


<210>  28
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  28
actagtacta gttaccaaat tgtttctcaa tcgttttaga gctagaaata gc               52


<210>  29
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  29
actagtacta gtgcaaaagc tggataagca gcgttttaga gctagaaata gc               52


<210>  30
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  30
actagtacta gtattgtcgc gcatctccac tggttttaga gctagaaata gc               52


<210>  31
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  31
actagtacta gtgttttgtt caatgcgatg cagttttaga gctagaaata gc               52


<210>  32
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  32
actagtacta gtccagtcca aaatgctatg aagttttaga gctagaaata gc               52


<210>  33
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  33
agatctagat ctaaagagga gaaaggatct atggataaga aatactcaat                  50


<210>  34
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  34
ctcgagctcg agttaacgac gaccttcagc aa                                     32


<210>  35
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  35
gggggggacg tctaagaaac cattattatc atg                                    33


<210>  36
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  36
gggcccgggc ccaagcttca aaaaaagcac cg                                     32


<210>  37
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  37
ctattgccag ggatggttc                                                    19


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  38
tttcatagaa atgcagcgcc                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  39
ggatgttgat tctgtcatgg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  40
tatgcattgc agaccttgtg                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  41
agtagacgtt atcgtcgttg                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  42
atggaacgtg agcaggacag                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  43
cagccagcgt ttcagcatac                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  44
tctgctgcga gacataaccc                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  45
acttgcaacg aatgttccgc                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  46
acgcactacc accagtaacg                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  47
tttgtcttcc gggaccagtg                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  48
aagcccttct tccagacgtg                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  49
aacggcaaag ccaaagtgtc                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  50
atcgcctggc tcatcatacg                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  51
gcactggaaa tctgtgacgc                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  52
ggccagtttg tgattacgcc                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  53
ctacgctccc acaaaatgcg                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  54
aatcgcgcaa aagctgaagg                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  55
gcgattcgcc ctgcatattg                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  56
aacacatgca agtcgaacgg                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  57
aatcccatct gggcacatcc                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  58
cgcagtcttg ctaccctttg                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  59
acgggcaaca tcttccacac                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  60
gcaagcgtat ctctcgtgaa                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  61
cgccgaacac acatttatcc                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  62
tcggacgcac ttttggtcag                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  63
caactggaac ctttcgggtg                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  64
tgatcaaccg gttgcggttc                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  65
atcttgcagc acttggacgg                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  66
aatacgccac ccgtgaagac                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  67
gacgccccat catgtaaccg                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  68
ggagagcgaa ttactctggc                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  69
ggttagcgaa cgtctgaatg                                                   20


<210>  70
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  70
ccgatacaat tttgcgtact tgc                                               23


<210>  71
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  71
ggctatgtta aaacgcatga acg                                               23


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  72
atacgcccat gatgcagcag                                                   20


<210>  73
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  73
cccatacgcc catgatgcag cag                                               23


<210>  74
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  74
gggtatgcgg gtactacgtc gtc                                               23


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  75
gccgcttaac attgatgtag                                                   20


<210>  76
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  76
ctacatcaat gttaagcggc tgg                                               23


<210>  77
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial seqeunce

<400>  77
ccagccgctt aacattgatg tag                                               23


<210>  78
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  78
ccccattctt atcggcctga gcc                                               23


<210>  79
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  79
ggctcaggcc gataagaatg ggg                                               23


<210>  80
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  80
agcatcagaa cgaccgccag agg                                               23


<210>  81
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  81
cctctggcgg tcgttctgat gct                                               23


<210>  82
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  82
taccaaattg tttctcaatc tgg                                               23


<210>  83
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  83
ccagattgag aaacaatttg gat                                               23


<210>  84
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  84
ccgcagtgga gatgcgcgac aat                                               23


<210>  85
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  85
attgtcgcgc atctccactg cgg                                               23


<210>  86
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  86
ccattcatag cattttggac tgg                                               23


<210>  87
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  87
ccagtccaaa atgctatgaa tgg                                               23


<210>  88
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  88
cccccggcga agtggcgaaa cgc                                               23


<210>  89
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  89
gcgtttcgcc acttcgccgg ggg                                               23


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence

<400>  90
ccggcgaagt ggcgaaacgc                                                   20


<210>  91
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  91
agcagtcatg tgttaaattg agg                                               23


<210>  92
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  92
cctcaattta acacatgact gct                                               23


<210>  93
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  93
ccaacacatc ctcgatgtgg ctc                                               23


<210>  94
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  94
gagccacatc gaggatgtgt tgg                                               23


<210>  95
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  95
cctgacgacg caccgcatca cgg                                               23


<210>  96
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  96
ccgtgatgcg gtgcgtcgtc agg                                               23


<210>  97
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  97
gcaaaagctg gataagcagc agg                                               23


<210>  98
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  98
cctgctgctt atccagcttt tgc                                               23


<210>  99
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  99
ccttgcatcg cattgaacaa aac                                               23


<210>  100
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  derived from E. coli

<400>  100
gttttgttca atgcgatgca agg                                               23


<210>  101
<211>  164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  partial plasmid vector sequence

<400>  101
agatctttga cagctagctc agtcctaggt ataatactag taactttcag tttagcggtc       60

tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag      120

tggcaccgag tcggtgcttt ttttgaagct tgggcccgaa caaa                       164


<210>  102
<211>  164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  partial plasmid vector sequence

<400>  102
tttgttcggg cccaagcttc aaaaaaagca ccgactcggt gccacttttt caagttgata       60

acggactagc cttattttaa cttgctattt ctagctctaa aacagaccgc taaactgaaa      120

gttactagta ttatacctag gactgagcta gctgtcaaag atct                       164


<210>  103
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  103
actagtacta gtgcaaaagc tggataagca gcgttttaga gctagaaata g                51


<210>  104
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial primer

<400>  104
gggcccgggc ccaagcttca aaaaaagcac cg                                     32


<210>  105
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence


<220>
<221>  N_region
<222>  (1)..(4)
<223>  n = any nucleic acid

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, or t

<220>
<221>  N_region
<222>  (10)..(12)
<223>  n = any nucleic acid

<220>
<221>  misc_feature
<222>  (11)..(13)
<223>  n is a, c, g, or t

<400>  105
nnnntctaga nnn                                                          13


<210>  106
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence


<220>
<221>  N_region
<222>  (1)..(3)
<223>  n = any nucleic acid

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  n is a, c, g, or t

<220>
<221>  N_region
<222>  (10)..(12)
<223>  n = any nucleic acid

<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  n is a, c, g, or t

<400>  106
nnntctagan nn                                                           12


<210>  107
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence


<220>
<221>  N_region
<222>  (1)..(3)
<223>  n = any nucleic acid

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  n is a, c, g, or t

<220>
<221>  N_region
<222>  (10)..(12)
<223>  n = any nucleic acid

<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  n is a, c, g, or t

<400>  107
nnncctaggn nn                                                           12


<210>  108
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial sequence


<220>
<221>  N_region
<222>  (1)..(3)
<223>  n = any nucleic acid

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  n is a, c, g, or t

<220>
<221>  N_region
<222>  (10)..(12)
<223>  n = any nucleic acid

<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  n is a, c, g, or t

<400>  108
nnncctaggn nn                                                           12


