                         SEQUENCE LISTING

<110>  Bristol-Myers Squibb Company
 
<120>  ANTIBODY-DRUG CONJUGATE OF AN ANTI-GLYPICAN-3 ANTIBODY AND A 
       TUBULYSIN ANALOG, PREPARATION AND USES

<130>  12526-WO-PCT

<150>  US 62/333,944
<151>  2016-05-10

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 heavy chain CDR1

<400>  1

Ser Tyr Trp Ile Ala 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 heavy chain CDR2

<400>  2

Ile Ile Phe Pro Gly Asp Ser Asp Thr Arg Tyr Ser Pro Ser Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 heavy chain CDR3

<400>  3

Thr Arg Glu Gly Tyr Phe Asp Tyr 
1               5               


<210>  4
<211>  12
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 kappa chain CDR1

<400>  4

Arg Ala Val Gln Ser Val Ser Ser Ser Tyr Leu Ala 
1               5                   10          


<210>  5
<211>  7
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 kappa chain CDR2

<400>  5

Gly Ala Ser Ser Arg Ala Thr 
1               5           


<210>  6
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 kappa chain CDR3

<400>  6

Gln Gln Tyr Gly Ser Ser Pro Thr 
1               5               


<210>  7
<211>  117
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 heavy chain variable region

<220>
<221>  MISC_FEATURE
<222>  (31)..(35)
<223>  GPC3.1 VH CDR1

<220>
<221>  MISC_FEATURE
<222>  (50)..(66)
<223>  GPC3.1 VH CDR2

<220>
<221>  MISC_FEATURE
<222>  (99)..(106)
<223>  GPC3.1 VH CDR3

<400>  7

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
            20                  25                  30          


Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Phe Pro Gly Asp Ser Asp Thr Arg Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly Gln Val Thr Ile Ser Ala Asp Arg Ser Ile Arg Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Thr Arg Glu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  8
<211>  107
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 kappa chain variable region

<220>
<221>  MISC_FEATURE
<222>  (24)..(34)
<223>  GPC3.1 VK CDR1

<220>
<221>  MISC_FEATURE
<222>  (51)..(57)
<223>  GPC3.1 VK CDR2

<220>
<221>  MISC_FEATURE
<222>  (90)..(97)
<223>  GPC3.1 VK CDR3

<400>  8

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Val Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  9
<211>  329
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GPC3.1 heavy chain constant region


<220>
<221>  MISC_FEATURE
<222>  (97)..(97)
<223>  R214 (EU/Kabat) allotype

<220>
<221>  MISC_FEATURE
<222>  (239)..(239)
<223>  E356 (EU/Kabat) allotype

<220>
<221>  MISC_FEATURE
<222>  (241)..(241)
<223>  M358 (EU/Kabat) allotype

<400>  9

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                325                 


<210>  10
<211>  107
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 kappa chain constant region

<400>  10

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  11
<211>  446
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GPC3.1 heavy chain

<400>  11

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
            20                  25                  30          


Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Phe Pro Gly Asp Ser Asp Thr Arg Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly Gln Val Thr Ile Ser Ala Asp Arg Ser Ile Arg Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Thr Arg Glu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445     


<210>  12
<211>  214
<212>  PRT
<213>  Homo Sapiens


<220>
<221>  MISC_FEATURE
<223>  GPC3.1 kappa chain

<400>  12

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Val Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  13
<211>  1392
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GPC3.1 heavy chain with signal peptide


<220>
<221>  sig_peptide
<222>  (1)..(51)

<220>
<221>  V_region
<222>  (52)..(402)

<220>
<221>  C_region
<222>  (403)..(1392)

<400>  13
atgagggctt ggatcttctt tctgctctgc ctggccggga gagcgctcgc agaggtgcag       60

ctggtgcagt ctggagcaga ggtgaaaaag cccggggagt ctctgaagat ctcctgtaag      120

ggttctggat acagctttac cagctactgg atcgcctggg tgcgccagat gcccgggaaa      180

ggcctggagt ggatggggat catctttcct ggtgactctg ataccagata cagcccgtcc      240

ttccaaggcc aggtcaccat ctcagccgac aggtccatca gaaccgccta cctgcagtgg      300

agcagcctga aggcctcgga caccgccttg tattactgtg cgagaacccg ggaggggtac      360

tttgactact ggggccaggg aaccctggtc accgtctcct cagctagcac caagggccca      420

tcggtcttcc ccctggcacc ctcctccaag agcacctctg ggggcacagc ggccctgggc      480

tgcctggtca aggactactt ccccgaaccg gtgacggtgt cgtggaactc aggcgccctg      540

accagcggcg tgcacacctt cccggctgtc ctacagtcct caggactcta ctccctcagc      600

agcgtggtga ccgtgccctc cagcagcttg ggcacccaga cctacatctg caacgtgaat      660

cacaagccca gcaacaccaa ggtggacaag agagttgagc ccaaatcttg tgacaaaact      720

cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt cttcctcttc      780

cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg      840

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag      900

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc      960

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc     1020

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc     1080

cgagaaccac aggtgtacac cctgccccca tcccgggagg agatgaccaa gaaccaggtc     1140

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc     1200

aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc     1260

ttcttcctct atagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc     1320

tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg     1380

tccccgggtt ga                                                         1392


<210>  14
<211>  463
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GPC3.1 heavy chain with signal peptide


<220>
<221>  SIGNAL
<222>  (1)..(17)

<400>  14

Met Arg Ala Trp Ile Phe Phe Leu Leu Cys Leu Ala Gly Arg Ala Leu 
1               5                   10                  15      


Ala Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly 
            20                  25                  30          


Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser 
        35                  40                  45              


Tyr Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp 
    50                  55                  60                  


Met Gly Ile Ile Phe Pro Gly Asp Ser Asp Thr Arg Tyr Ser Pro Ser 
65                  70                  75                  80  


Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Arg Ser Ile Arg Thr Ala 
                85                  90                  95      


Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Leu Tyr Tyr 
            100                 105                 110         


Cys Ala Arg Thr Arg Glu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
    130                 135                 140                 


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
145                 150                 155                 160 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
                165                 170                 175     


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
            180                 185                 190         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
        195                 200                 205             


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
    210                 215                 220                 


Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr 
225                 230                 235                 240 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
                245                 250                 255     


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
            260                 265                 270         


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
        275                 280                 285             


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
    290                 295                 300                 


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
305                 310                 315                 320 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
                325                 330                 335     


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
            340                 345                 350         


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
        355                 360                 365             


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
    370                 375                 380                 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
385                 390                 395                 400 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
                405                 410                 415     


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
            420                 425                 430         


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
        435                 440                 445             


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
    450                 455                 460             


<210>  15
<211>  696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GPC3.1 kappa chain with signal peptide


<220>
<221>  sig_peptide
<222>  (1)..(51)

<220>
<221>  V_region
<222>  (52)..(372)

<220>
<221>  C_region
<222>  (373)..(696)

<400>  15
atgagggctt ggatcttctt tctgctctgc ctggccgggc gcgccttggc cgaaattgtg       60

ttgacgcagt ctccaggcac cctgtctttg tctccagggg aaagagccac cctctcctgc      120

agggccgttc agagtgttag cagcagctat ttagcctggt accagcagaa acctggccag      180

gctcccaggc tcctcatcta tggtgcatcc agcagggcca ctggcatccc agacaggttc      240

agtggcagtg ggtctgggac agacttcact ctcaccatca gcagactgga gcctgaagat      300

tttgcagtgt attactgtca gcagtatggt agctcaccca ctttcggcgg agggaccaag      360

gtggagatca aacgtacggt ggctgcacca tctgtcttca tcttcccgcc atctgatgag      420

cagttgaaat ctggaactgc ctctgttgtg tgcctgctga ataacttcta tcccagagag      480

gccaaagtac agtggaaggt ggataacgcc ctccaatcgg gtaactccca ggagagtgtc      540

acagagcagg acagcaagga cagcacctac agcctcagca gcaccctgac gctgagcaaa      600

gcagactacg agaaacacaa agtctacgcc tgcgaagtca cccatcaggg cctgagctcg      660

cccgtcacaa agagcttcaa caggggagag tgttag                                696


<210>  16
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GPC3.1 kappa chain with signal peptide


<220>
<221>  SIGNAL
<222>  (1)..(17)

<400>  16

Met Arg Ala Trp Ile Phe Phe Leu Leu Cys Leu Ala Gly Arg Ala Leu 
1               5                   10                  15      


Ala Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro 
            20                  25                  30          


Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Val Gln Ser Val Ser Ser 
        35                  40                  45              


Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu 
    50                  55                  60                  


Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu 
                85                  90                  95      


Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser 
            100                 105                 110         


Pro Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala 
        115                 120                 125             


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
    130                 135                 140                 


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
145                 150                 155                 160 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
                165                 170                 175     


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
            180                 185                 190         


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
        195                 200                 205             


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
    210                 215                 220                 


Ser Phe Asn Arg Gly Glu Cys 
225                 230     


<210>  17
<211>  17
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibodies A and C heavy chain CDR2

<400>  17

Ile Ile Phe Pro Gly Asp Ser His Thr Gly Tyr Ser Pro Ser Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  18
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody A heavy chain CDR3

<400>  18

Thr Arg Gln Gly Tyr Phe Asp Tyr 
1               5               


<210>  19
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibodies A and C kappa chain CDR3

<400>  19

Gln Gln Tyr Gly Ser Leu Pro Thr 
1               5               


<210>  20
<211>  5
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibodies B and C heavy chain CDR1

<400>  20

Arg Tyr Trp Ile Ala 
1               5   


<210>  21
<211>  17
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody B heavy chain CDR2

<400>  21

Ile Ile Phe Pro Gly Asp Ser His Thr Gly Tyr Ser Pro Ser Phe Arg 
1               5                   10                  15      


Gly 
    


<210>  22
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody B kappa chain CDR3

<400>  22

Gln Gln Tyr Gly Arg Ser Pro Thr 
1               5               


<210>  23
<211>  117
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody A heavy chain variable region

<400>  23

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Ser Tyr 
            20                  25                  30          


Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Phe Pro Gly Asp Ser His Thr Gly Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly Gln Val Thr Ile Ser Ala Asp Arg Ser Ile Arg Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Thr Arg Gln Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  24
<211>  107
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody A kappa chain variable region

<400>  24

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Val Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Leu Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  25
<211>  117
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody B heavy chain variable region

<400>  25

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Arg Tyr 
            20                  25                  30          


Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Phe Pro Gly Asp Ser His Thr Gly Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Arg Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Thr Arg Glu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  26
<211>  107
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody B kappa chain variable region

<400>  26

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Val Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Arg Ser Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  27
<211>  117
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody C heavy chain variable region

<400>  27

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Arg Tyr 
            20                  25                  30          


Trp Ile Ala Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Phe Pro Gly Asp Ser His Thr Gly Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly Gln Val Thr Ile Ser Ala Asp Arg Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Thr Arg Glu Gly Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  28
<211>  107
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Antibody C kappa chain variable region

<400>  28

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Val Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Leu Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


