                         SEQUENCE LISTING

<110>  Universit de Lorraine
       CHRU de Nancy
       KRIZNIK, Alexandre
       REBOUL, Pascal
       YELEHE-OKOUMA, Mlissa Jenner
 
<120>  METHODE DE PURIFICATION DE PROTEINES RECOMBINANTES PAR AFFINITE 
       BASEE SUR L'ACTIVITE LECTINIQUE DU DOMAINE CRD DE LA GALECTINE-3 
       HUMAINE

<130>  BNT220567PC00

<140>  FR1654324
<141>  2016-05-13

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  157
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa est mthionine ou rien

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa est glycine, ou rien dans la version optimise du CRDSAT

<220>
<221>  MISC_FEATURE
<222>  (36)..(36)
<223>  Xaa est arginine, ou lysine dans la version optimise du CRDSAT

<220>
<221>  MISC_FEATURE
<222>  (152)..(152)
<223>  Xaa est alanine, ou thronine dans la version optimise du CRDSAT

<400>  1

Xaa Xaa Ser Ala Thr Gly Ala Tyr Pro Ala Thr Gly Pro Tyr Gly Ala 
1               5                   10                  15      


Pro Ala Gly Pro Leu Ile Val Pro Tyr Asn Leu Pro Leu Pro Gly Gly 
            20                  25                  30          


Val Val Pro Xaa Met Leu Ile Thr Ile Leu Gly Thr Val Lys Pro Asn 
        35                  40                  45              


Ala Asn Arg Ile Ala Leu Asp Phe Gln Arg Gly Asn Asp Val Ala Phe 
    50                  55                  60                  


His Phe Asn Pro Arg Phe Asn Glu Asn Asn Arg Arg Val Ile Val Cys 
65                  70                  75                  80  


Asn Thr Lys Leu Asp Asn Asn Trp Gly Arg Glu Glu Arg Gln Ser Val 
                85                  90                  95      


Phe Pro Phe Glu Ser Gly Lys Pro Phe Lys Ile Gln Val Leu Val Glu 
            100                 105                 110         


Pro Asp His Phe Lys Val Ala Val Asn Asp Ala His Leu Leu Gln Tyr 
        115                 120                 125             


Asn His Arg Val Lys Lys Leu Asn Glu Ile Ser Lys Leu Gly Ile Ser 
    130                 135                 140                 


Gly Asp Ile Asp Leu Thr Ser Xaa Ser Tyr Thr Met Ile 
145                 150                 155         


<210>  2
<211>  807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Squence partielle du vecteur pCARGHO


<220>
<221>  CDS
<222>  (94)..(690)

<400>  2
gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat       60

aattttgttt aactttaaga aggagatata cat atg agc gca acc ggt gca tat       114
                                     Met Ser Ala Thr Gly Ala Tyr          
                                     1               5                    

ccg gca acc ggt ccg tat ggt gca ccg gca ggt ccg ctg att gtt ccg        162
Pro Ala Thr Gly Pro Tyr Gly Ala Pro Ala Gly Pro Leu Ile Val Pro           
        10                  15                  20                        

tat aat ctg ccg ctg cct ggt ggt gtt gtt ccg aaa atg ctg att acc        210
Tyr Asn Leu Pro Leu Pro Gly Gly Val Val Pro Lys Met Leu Ile Thr           
    25                  30                  35                            

att ctg ggc acc gtt aaa ccg aat gca aat cgt att gca ctg gat ttt        258
Ile Leu Gly Thr Val Lys Pro Asn Ala Asn Arg Ile Ala Leu Asp Phe           
40                  45                  50                  55            

cag cgt ggt aat gat gtg gcc ttt cat ttt aat ccg cgt ttc aat gaa        306
Gln Arg Gly Asn Asp Val Ala Phe His Phe Asn Pro Arg Phe Asn Glu           
                60                  65                  70                

aac aac cgt cgt gtt att gtg tgc aat acc aaa ctg gat aac aat tgg        354
Asn Asn Arg Arg Val Ile Val Cys Asn Thr Lys Leu Asp Asn Asn Trp           
            75                  80                  85                    

ggt cgt gaa gaa cgt cag agc gtt ttt ccg ttt gaa agc ggt aaa ccg        402
Gly Arg Glu Glu Arg Gln Ser Val Phe Pro Phe Glu Ser Gly Lys Pro           
        90                  95                  100                       

ttt aaa atc cag gtt ctg gtt gaa ccg gat cat ttt aaa gtt gca gtt        450
Phe Lys Ile Gln Val Leu Val Glu Pro Asp His Phe Lys Val Ala Val           
    105                 110                 115                           

aat gat gca cat ctg ctg cag tat aat cac cgt gtg aaa aaa ctg aac        498
Asn Asp Ala His Leu Leu Gln Tyr Asn His Arg Val Lys Lys Leu Asn           
120                 125                 130                 135           

gag att agc aaa ctg ggt atc agc ggt gat att gat ctg acc agc acc        546
Glu Ile Ser Lys Leu Gly Ile Ser Gly Asp Ile Asp Leu Thr Ser Thr           
                140                 145                 150               

agc tat acc atg att agc agc ggt gtt gat ctg ggt aca gaa aat ctg        594
Ser Tyr Thr Met Ile Ser Ser Gly Val Asp Leu Gly Thr Glu Asn Leu           
            155                 160                 165                   

tat ttt cag agc gcc atg gat atc aat tcg gat ccg aat tcg agc tcc        642
Tyr Phe Gln Ser Ala Met Asp Ile Asn Ser Asp Pro Asn Ser Ser Ser           
        170                 175                 180                       

gtc gac aag ctt gcg gcc gca ctc gag cac cac cac cac cac cac tga        690
Val Asp Lys Leu Ala Ala Ala Leu Glu His His His His His His               
    185                 190                 195                           

gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa      750

taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaa         807


<210>  3
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3

Met Ser Ala Thr Gly Ala Tyr Pro Ala Thr Gly Pro Tyr Gly Ala Pro 
1               5                   10                  15      


Ala Gly Pro Leu Ile Val Pro Tyr Asn Leu Pro Leu Pro Gly Gly Val 
            20                  25                  30          


Val Pro Lys Met Leu Ile Thr Ile Leu Gly Thr Val Lys Pro Asn Ala 
        35                  40                  45              


Asn Arg Ile Ala Leu Asp Phe Gln Arg Gly Asn Asp Val Ala Phe His 
    50                  55                  60                  


Phe Asn Pro Arg Phe Asn Glu Asn Asn Arg Arg Val Ile Val Cys Asn 
65                  70                  75                  80  


Thr Lys Leu Asp Asn Asn Trp Gly Arg Glu Glu Arg Gln Ser Val Phe 
                85                  90                  95      


Pro Phe Glu Ser Gly Lys Pro Phe Lys Ile Gln Val Leu Val Glu Pro 
            100                 105                 110         


Asp His Phe Lys Val Ala Val Asn Asp Ala His Leu Leu Gln Tyr Asn 
        115                 120                 125             


His Arg Val Lys Lys Leu Asn Glu Ile Ser Lys Leu Gly Ile Ser Gly 
    130                 135                 140                 


Asp Ile Asp Leu Thr Ser Thr Ser Tyr Thr Met Ile Ser Ser Gly Val 
145                 150                 155                 160 


Asp Leu Gly Thr Glu Asn Leu Tyr Phe Gln Ser Ala Met Asp Ile Asn 
                165                 170                 175     


Ser Asp Pro Asn Ser Ser Ser Val Asp Lys Leu Ala Ala Ala Leu Glu 
            180                 185                 190         


His His His His His His 
        195             


<210>  4
<211>  250
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ala Asp Asn Phe Ser Leu His Asp Ala Leu Ser Gly Ser Gly Asn 
1               5                   10                  15      


Pro Asn Pro Gln Gly Trp Pro Gly Ala Trp Gly Asn Gln Pro Ala Gly 
            20                  25                  30          


Ala Gly Gly Tyr Pro Gly Ala Ser Tyr Pro Gly Ala Tyr Pro Gly Gln 
        35                  40                  45              


Ala Pro Pro Gly Ala Tyr Pro Gly Gln Ala Pro Pro Gly Ala Tyr Pro 
    50                  55                  60                  


Gly Ala Pro Gly Ala Tyr Pro Gly Ala Pro Ala Pro Gly Val Tyr Pro 
65                  70                  75                  80  


Gly Pro Pro Ser Gly Pro Gly Ala Tyr Pro Ser Ser Gly Gln Pro Ser 
                85                  90                  95      


Ala Thr Gly Ala Tyr Pro Ala Thr Gly Pro Tyr Gly Ala Pro Ala Gly 
            100                 105                 110         


Pro Leu Ile Val Pro Tyr Asn Leu Pro Leu Pro Gly Gly Val Val Pro 
        115                 120                 125             


Arg Met Leu Ile Thr Ile Leu Gly Thr Val Lys Pro Asn Ala Asn Arg 
    130                 135                 140                 


Ile Ala Leu Asp Phe Gln Arg Gly Asn Asp Val Ala Phe His Phe Asn 
145                 150                 155                 160 


Pro Arg Phe Asn Glu Asn Asn Arg Arg Val Ile Val Cys Asn Thr Lys 
                165                 170                 175     


Leu Asp Asn Asn Trp Gly Arg Glu Glu Arg Gln Ser Val Phe Pro Phe 
            180                 185                 190         


Glu Ser Gly Lys Pro Phe Lys Ile Gln Val Leu Val Glu Pro Asp His 
        195                 200                 205             


Phe Lys Val Ala Val Asn Asp Ala His Leu Leu Gln Tyr Asn His Arg 
    210                 215                 220                 


Val Lys Lys Leu Asn Glu Ile Ser Lys Leu Gly Ile Ser Gly Asp Ile 
225                 230                 235                 240 


Asp Leu Thr Ser Thr Ser Tyr Thr Met Ile 
                245                 250 


<210>  5
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  5

Gly Gly Val Val Pro 
1               5   


<210>  6
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  6

Leu Ile Thr Ile Leu 
1               5   


<210>  7
<211>  155
<212>  PRT
<213>  Gorilla gorilla

<400>  7

Ser Ala Pro Gly Ala Tyr Pro Ala Thr Gly Pro Tyr Gly Ala Pro Ala 
1               5                   10                  15      


Gly Pro Leu Ile Val Pro Tyr Asn Leu Pro Leu Pro Gly Gly Val Val 
            20                  25                  30          


Pro Arg Met Leu Ile Thr Ile Leu Gly Thr Val Lys Pro Asn Ala Asn 
        35                  40                  45              


Arg Ile Ala Leu Asp Phe Gln Arg Gly Asn Asp Val Ala Phe His Phe 
    50                  55                  60                  


Asn Pro Arg Phe Asn Glu Asn Asn Arg Arg Val Ile Val Cys Asn Thr 
65                  70                  75                  80  


Lys Leu Asp Asn Asn Trp Gly Arg Glu Glu Arg Gln Ser Val Phe Pro 
                85                  90                  95      


Phe Glu Ser Gly Lys Pro Phe Lys Ile Gln Val Leu Val Glu Pro Asp 
            100                 105                 110         


His Phe Lys Val Ala Val Asn Asp Ala His Leu Leu Gln Tyr Asn His 
        115                 120                 125             


Arg Val Lys Lys Leu Asn Glu Ile Ser Lys Leu Gly Ile Ser Gly Asp 
    130                 135                 140                 


Ile Asp Leu Thr Ser Ala Ser Tyr Asn Met Ile 
145                 150                 155 


<210>  8
<211>  155
<212>  PRT
<213>  Felis catus

<400>  8

Ser Ala Pro Gly Ala Tyr Pro Ala Ala Gly Pro Phe Gly Val Pro Ala 
1               5                   10                  15      


Gly Pro Leu Thr Val Pro Tyr Asp Leu Pro Leu Pro Gly Gly Val Met 
            20                  25                  30          


Pro Arg Met Leu Ile Thr Ile Leu Gly Thr Val Lys Pro Asn Ala Asn 
        35                  40                  45              


Arg Leu Ala Leu Asp Phe Lys Arg Gly Asn Asp Val Ala Phe His Phe 
    50                  55                  60                  


Asn Pro Arg Phe Asn Glu Asp Asn Lys Arg Val Ile Val Cys Asn Thr 
65                  70                  75                  80  


Lys Leu Glu Asn Leu Trp Gly Lys Glu Glu Arg Gln Ser Thr Phe Pro 
                85                  90                  95      


Phe Glu Ser Gly Lys Pro Phe Lys Ile Gln Val Leu Val Glu Ser Asp 
            100                 105                 110         


His Phe Lys Val Ala Val Asn Asp Ala His Leu Leu Gln Tyr Asn His 
        115                 120                 125             


Arg Met Lys Asn Leu His Glu Ile Ser Lys Leu Gly Ile Ser Gly Asp 
    130                 135                 140                 


Ile Asp Leu Thr Ser Ala Ser His Thr Met Ile 
145                 150                 155 


<210>  9
<211>  155
<212>  PRT
<213>  Bos taurus

<400>  9

Ser Ala Pro Gly Ala Tyr Pro Ala Ala Gly Pro Tyr Gly Ile Pro Ser 
1               5                   10                  15      


Gly Pro Leu Asn Val Pro Tyr Asp Leu Pro Phe Pro Gly Gly Ile Arg 
            20                  25                  30          


Pro Arg Met Leu Ile Thr Ile Leu Gly Thr Val Lys Pro Asn Ala Asn 
        35                  40                  45              


Arg Leu Ala Leu Asp Phe Lys Arg Gly Asn Asp Val Ala Phe His Phe 
    50                  55                  60                  


Asn Pro Arg Phe Asn Glu Asp Asn Arg Arg Val Ile Val Cys Asn Ser 
65                  70                  75                  80  


Lys Leu Asn Asn Asn Trp Gly Lys Glu Glu Arg Gln Met Val Phe Pro 
                85                  90                  95      


Phe Glu Ser Gly Lys Pro Phe Lys Ile Gln Val Leu Val Glu Pro Asp 
            100                 105                 110         


His Phe Lys Val Ala Val Asn Asp Ala His Leu Leu Gln Tyr Asn His 
        115                 120                 125             


Arg Val Lys Asn Phe Gly Glu Ile Ser Thr Leu Gly Ile Ser Gly Asp 
    130                 135                 140                 


Ile Thr Leu Thr Ser Ala Ser His Thr Met Ile 
145                 150                 155 


<210>  10
<211>  155
<212>  PRT
<213>  Mus musculus

<400>  10

Ser Ala Pro Gly Gly Tyr Pro Ala Ala Gly Pro Tyr Gly Val Pro Ala 
1               5                   10                  15      


Gly Pro Leu Thr Val Pro Tyr Asp Leu Pro Leu Pro Gly Gly Val Met 
            20                  25                  30          


Pro Arg Met Leu Ile Thr Ile Met Gly Thr Val Lys Pro Asn Ala Asn 
        35                  40                  45              


Arg Ile Val Leu Asp Phe Arg Arg Gly Asn Asp Val Ala Phe His Phe 
    50                  55                  60                  


Asn Pro Arg Phe Asn Glu Asn Asn Arg Arg Val Ile Val Cys Asn Thr 
65                  70                  75                  80  


Lys Gln Asp Asn Asn Trp Gly Lys Glu Glu Arg Gln Ser Ala Phe Pro 
                85                  90                  95      


Phe Glu Ser Gly Lys Pro Phe Lys Ile Gln Val Leu Val Glu Ala Asp 
            100                 105                 110         


His Phe Lys Val Ala Val Asn Asp Ala His Leu Leu Gln Tyr Asn His 
        115                 120                 125             


Arg Met Lys Asn Leu Arg Glu Ile Ser Gln Leu Gly Ile Ser Gly Asp 
    130                 135                 140                 


Ile Thr Leu Thr Ser Ala Asn His Ala Met Ile 
145                 150                 155 


<210>  11
<211>  155
<212>  PRT
<213>  Rattus norvegicus

<400>  11

Ser Ala Pro Gly Ala Tyr Pro Ala Thr Gly Pro Phe Gly Ala Pro Thr 
1               5                   10                  15      


Gly Pro Leu Thr Val Pro Tyr Asp Met Pro Leu Pro Gly Gly Val Met 
            20                  25                  30          


Pro Arg Met Leu Ile Thr Ile Ile Gly Thr Val Lys Pro Asn Ala Asn 
        35                  40                  45              


Ser Ile Thr Leu Asn Phe Lys Arg Gly Asn Asp Ile Ala Phe His Phe 
    50                  55                  60                  


Asn Pro Arg Phe Asn Glu Asn Asn Arg Arg Val Ile Val Cys Asn Thr 
65                  70                  75                  80  


Lys Gln Asp Asn Asn Trp Gly Arg Glu Glu Arg Gln Ser Ala Phe Pro 
                85                  90                  95      


Phe Glu Ser Gly Lys Pro Phe Lys Ile Gln Val Leu Val Glu Ala Asp 
            100                 105                 110         


His Phe Lys Val Ala Val Asn Asp Val His Leu Leu Gln Tyr Asn His 
        115                 120                 125             


Arg Met Lys Asn Leu Arg Glu Ile Ser Gln Leu Gly Ile Ile Gly Asp 
    130                 135                 140                 


Ile Thr Leu Thr Ser Ala Ser His Ala Met Ile 
145                 150                 155 


