                         SEQUENCE LISTING

<110>  Oslo universitetssykehus HF
 
<120>  T-Cell Receptors which Recognise Frameshift Mutants of TGFBRII

<130>  27.18.123119/01

<150>  GB 1608052.5
<151>  2016-05-09

<160>  82    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  1

Arg Leu Ser Ser Cys Val Pro Val Ala 
1               5                   


<210>  2
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  2

Asp Ser Val Asn Asn 
1               5   


<210>  3
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  3

Ile Pro Ser Gly Thr 
1               5   


<210>  4
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  4

Cys Ala Val Asn Ala Gly Asn Met Leu Thr Phe 
1               5                   10      


<210>  5
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Asp His Glu Asn 
1               5   


<210>  6
<211>  6
<212>  PRT
<213>  Homo sapiens

<400>  6

Ser Tyr Asp Val Lys Met 
1               5       


<210>  7
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  7

Cys Ala Ser Ser Ser Gly Val Thr Gly Glu Leu Phe Phe 
1               5                   10              


<210>  8
<211>  127
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro 
        115                 120                 125         


<210>  9
<211>  141
<212>  PRT
<213>  Homo sapiens

<400>  9

His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys 
1               5                   10                  15      


Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr 
            20                  25                  30          


Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr 
        35                  40                  45              


Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala 
    50                  55                  60                  


Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser 
65                  70                  75                  80  


Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp 
                85                  90                  95      


Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe 
            100                 105                 110         


Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala 
        115                 120                 125             


Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135                 140     


<210>  10
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified Radium-1 alpha-chain constant region

<400>  10

His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys 
1               5                   10                  15      


Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr 
            20                  25                  30          


Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys 
        35                  40                  45              


Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala 
    50                  55                  60                  


Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser 
65                  70                  75                  80  


Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp 
                85                  90                  95      


Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe 
            100                 105                 110         


Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala 
        115                 120                 125             


Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135                 140     


<210>  11
<211>  268
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His 
        115                 120                 125             


Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
    130                 135                 140                 


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
145                 150                 155                 160 


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
                165                 170                 175     


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
            180                 185                 190         


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
        195                 200                 205             


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp Val 
    210                 215                 220                 


Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
225                 230                 235                 240 


Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
                245                 250                 255     


Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
            260                 265             


<210>  12
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified Radium-1 alpha-chain

<400>  12

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His 
        115                 120                 125             


Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
    130                 135                 140                 


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
145                 150                 155                 160 


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val 
                165                 170                 175     


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
            180                 185                 190         


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
        195                 200                 205             


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp Val 
    210                 215                 220                 


Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
225                 230                 235                 240 


Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
                245                 250                 255     


Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
            260                 265             


<210>  13
<211>  131
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala Val 
1               5                   10                  15      


Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
            20                  25                  30          


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
        35                  40                  45              


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
    50                  55                  60                  


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
65                  70                  75                  80  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
                85                  90                  95      


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
        115                 120                 125             


Thr Val Leu 
    130     


<210>  14
<211>  179
<212>  PRT
<213>  Homo sapiens

<400>  14

Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser 
    130                 135                 140                 


Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala 
145                 150                 155                 160 


Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp 
                165                 170                 175     


Ser Arg Gly 
            


<210>  15
<211>  179
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified Radium-1 beta-chain constant region

<400>  15

Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser 
    130                 135                 140                 


Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala 
145                 150                 155                 160 


Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp 
                165                 170                 175     


Ser Arg Gly 
            


<210>  16
<211>  310
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala Val 
1               5                   10                  15      


Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
            20                  25                  30          


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
        35                  40                  45              


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
    50                  55                  60                  


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
65                  70                  75                  80  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
                85                  90                  95      


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
        115                 120                 125             


Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val 
    130                 135                 140                 


Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu 
145                 150                 155                 160 


Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp 
                165                 170                 175     


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln 
            180                 185                 190         


Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser 
        195                 200                 205             


Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His 
    210                 215                 220                 


Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp 
225                 230                 235                 240 


Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala 
                245                 250                 255     


Trp Gly Arg Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly 
            260                 265                 270         


Val Leu Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr 
        275                 280                 285             


Leu Tyr Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys 
    290                 295                 300                 


Arg Lys Asp Ser Arg Gly 
305                 310 


<210>  17
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified Radium-1 beta-chain

<400>  17

Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala Val 
1               5                   10                  15      


Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
            20                  25                  30          


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
        35                  40                  45              


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
    50                  55                  60                  


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
65                  70                  75                  80  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
                85                  90                  95      


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
        115                 120                 125             


Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val 
    130                 135                 140                 


Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu 
145                 150                 155                 160 


Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp 
                165                 170                 175     


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln 
            180                 185                 190         


Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser 
        195                 200                 205             


Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His 
    210                 215                 220                 


Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp 
225                 230                 235                 240 


Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala 
                245                 250                 255     


Trp Gly Arg Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly 
            260                 265                 270         


Val Leu Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr 
        275                 280                 285             


Leu Tyr Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys 
    290                 295                 300                 


Arg Lys Asp Ser Arg Gly 
305                 310 


<210>  18
<211>  26
<212>  PRT
<213>  picornavirus

<400>  18

Arg Ala Lys Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln 
1               5                   10                  15      


Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            20                  25      


<210>  19
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Double Myc-tag

<400>  19

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln Lys Leu Ile Ser 
1               5                   10                  15      


Glu Glu Asp Leu 
            20  


<210>  20
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 alpha-chain variable region with double Myc-tag

<400>  20

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro 
145         


<210>  21
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 alpha-chain with double Myc-tag

<400>  21

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg 
145                 150                 155                 160 


Asp Ser Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp 
                165                 170                 175     


Ser Gln Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr 
            180                 185                 190         


Asp Lys Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser 
        195                 200                 205             


Ala Val Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe 
    210                 215                 220                 


Asn Asn Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser 
225                 230                 235                 240 


Ser Cys Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn 
                245                 250                 255     


Leu Asn Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu 
            260                 265                 270         


Lys Val Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
        275                 280                 285             


<210>  22
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified Radium-1 alpha-chain with double Myc-tag

<400>  22

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg 
145                 150                 155                 160 


Asp Ser Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp 
                165                 170                 175     


Ser Gln Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr 
            180                 185                 190         


Asp Lys Cys Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser 
        195                 200                 205             


Ala Val Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe 
    210                 215                 220                 


Asn Asn Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser 
225                 230                 235                 240 


Ser Cys Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn 
                245                 250                 255     


Leu Asn Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu 
            260                 265                 270         


Lys Val Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
        275                 280                 285             


<210>  23
<211>  136
<212>  PRT
<213>  Mus musculus

<400>  23

Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser 
1               5                   10                  15      


Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn 
            20                  25                  30          


Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Thr Val 
        35                  40                  45              


Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp 
    50                  55                  60                  


Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn 
65                  70                  75                  80  


Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu 
                85                  90                  95      


Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser Val 
            100                 105                 110         


Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu 
        115                 120                 125             


Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135     


<210>  24
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified murinised Radium-1 alpha chain constant region

<400>  24

Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser 
1               5                   10                  15      


Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn 
            20                  25                  30          


Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Cys Val 
        35                  40                  45              


Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp 
    50                  55                  60                  


Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn 
65                  70                  75                  80  


Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu 
                85                  90                  95      


Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser Val 
            100                 105                 110         


Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu 
        115                 120                 125             


Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135     


<210>  25
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 alpha-chain with murinised constant region

<400>  25

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro Ile 
        115                 120                 125             


Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser Gln 
    130                 135                 140                 


Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn Val 
145                 150                 155                 160 


Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Thr Val Leu 
                165                 170                 175     


Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp Ser 
            180                 185                 190         


Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn Ala 
        195                 200                 205             


Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu Lys 
    210                 215                 220                 


Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser Val Met 
225                 230                 235                 240 


Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu Met 
                245                 250                 255     


Thr Leu Arg Leu Trp Ser Ser 
            260             


<210>  26
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 alpha-chain with cysteine-modified murinised constant 
       region

<400>  26

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro Ile 
        115                 120                 125             


Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser Gln 
    130                 135                 140                 


Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn Val 
145                 150                 155                 160 


Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Cys Val Leu 
                165                 170                 175     


Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp Ser 
            180                 185                 190         


Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn Ala 
        195                 200                 205             


Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu Lys 
    210                 215                 220                 


Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser Val Met 
225                 230                 235                 240 


Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu Met 
                245                 250                 255     


Thr Leu Arg Leu Trp Ser Ser 
            260             


<210>  27
<211>  283
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 alpha chain with double Myc-tag and murinised constant 
       region

<400>  27

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp 
145                 150                 155                 160 


Pro Arg Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser 
                165                 170                 175     


Gln Ile Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp 
            180                 185                 190         


Lys Thr Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala 
        195                 200                 205             


Ile Ala Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys 
    210                 215                 220                 


Glu Thr Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr 
225                 230                 235                 240 


Leu Thr Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn 
                245                 250                 255     


Leu Ser Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe 
            260                 265                 270         


Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
        275                 280             


<210>  28
<211>  283
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 alpha-chain with double Myc-tag and cysteine-modified 
       murinised constant region

<400>  28

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp 
145                 150                 155                 160 


Pro Arg Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser 
                165                 170                 175     


Gln Ile Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp 
            180                 185                 190         


Lys Cys Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala 
        195                 200                 205             


Ile Ala Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys 
    210                 215                 220                 


Glu Thr Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr 
225                 230                 235                 240 


Leu Thr Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn 
                245                 250                 255     


Leu Ser Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe 
            260                 265                 270         


Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
        275                 280             


<210>  29
<211>  125
<212>  PRT
<213>  Mus musculus

<400>  29

Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser Leu Phe Glu Pro 
1               5                   10                  15      


Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Ala Tyr Lys 
    50                  55                  60                  


Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala 
65                  70                  75                  80  


Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe 
                85                  90                  95      


His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser Pro Lys Pro 
            100                 105                 110         


Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala 
        115                 120                 125 


<210>  30
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified murinised Radium-1 beta-chain constant region

<400>  30

Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser Leu Phe Glu Pro 
1               5                   10                  15      


Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Ala Tyr Lys 
    50                  55                  60                  


Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala 
65                  70                  75                  80  


Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe 
                85                  90                  95      


His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser Pro Lys Pro 
            100                 105                 110         


Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala 
        115                 120                 125 


<210>  31
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 beta-chain with murinised constant region

<400>  31

Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala Val 
1               5                   10                  15      


Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
            20                  25                  30          


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
        35                  40                  45              


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
    50                  55                  60                  


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
65                  70                  75                  80  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
                85                  90                  95      


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
        115                 120                 125             


Thr Val Leu Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser Leu 
    130                 135                 140                 


Phe Glu Pro Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu 
145                 150                 155                 160 


Val Cys Leu Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp 
                165                 170                 175     


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln 
            180                 185                 190         


Ala Tyr Lys Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg 
        195                 200                 205             


Val Ser Ala Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln 
    210                 215                 220                 


Val Gln Phe His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser 
225                 230                 235                 240 


Pro Lys Pro Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala 
                245                 250                 255     


<210>  32
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 beta-chain with cysteine-modified murinised constant 
       region

<400>  32

Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala Val 
1               5                   10                  15      


Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
            20                  25                  30          


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
        35                  40                  45              


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
    50                  55                  60                  


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
65                  70                  75                  80  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
                85                  90                  95      


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
        115                 120                 125             


Thr Val Leu Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser Leu 
    130                 135                 140                 


Phe Glu Pro Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu 
145                 150                 155                 160 


Val Cys Leu Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp 
                165                 170                 175     


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln 
            180                 185                 190         


Ala Tyr Lys Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg 
        195                 200                 205             


Val Ser Ala Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln 
    210                 215                 220                 


Val Gln Phe His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser 
225                 230                 235                 240 


Pro Lys Pro Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala 
                245                 250                 255     


<210>  33
<211>  604
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 scTCR with 2A linker

<400>  33

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His 
        115                 120                 125             


Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
    130                 135                 140                 


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
145                 150                 155                 160 


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
                165                 170                 175     


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
            180                 185                 190         


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
        195                 200                 205             


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp Val 
    210                 215                 220                 


Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
225                 230                 235                 240 


Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
                245                 250                 255     


Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser Arg Ala Lys Arg 
            260                 265                 270         


Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
        275                 280                 285             


Glu Glu Asn Pro Gly Pro Met Gly Ile Arg Leu Leu Cys Arg Val Ala 
    290                 295                 300                 


Phe Cys Phe Leu Ala Val Gly Leu Val Asp Val Lys Val Thr Gln Ser 
305                 310                 315                 320 


Ser Arg Tyr Leu Val Lys Arg Thr Gly Glu Lys Val Phe Leu Glu Cys 
                325                 330                 335     


Val Gln Asp Met Asp His Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro 
            340                 345                 350         


Gly Leu Gly Leu Arg Leu Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys 
        355                 360                 365             


Glu Lys Gly Asp Ile Pro Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys 
    370                 375                 380                 


Glu Arg Phe Ser Leu Ile Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser 
385                 390                 395                 400 


Met Tyr Leu Cys Ala Ser Ser Ser Gly Val Thr Gly Glu Leu Phe Phe 
                405                 410                 415     


Gly Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu Lys Asn Val Phe 
            420                 425                 430         


Pro Pro Glu Val Ala Val Phe Glu Pro Ser Glu Ala Glu Ile Ser His 
        435                 440                 445             


Thr Gln Lys Ala Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp 
    450                 455                 460                 


His Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly 
465                 470                 475                 480 


Val Ser Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp 
                485                 490                 495     


Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp 
            500                 505                 510         


Gln Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu 
        515                 520                 525             


Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys Pro Val Thr Gln 
    530                 535                 540                 


Ile Val Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly Phe Thr Ser 
545                 550                 555                 560 


Glu Ser Tyr Gln Gln Gly Val Leu Ser Ala Thr Ile Leu Tyr Glu Ile 
                565                 570                 575     


Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val Ser Ala Leu Val 
            580                 585                 590         


Leu Met Ala Met Val Lys Arg Lys Asp Ser Arg Gly 
        595                 600                 


<210>  34
<211>  604
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified Radium-1 scTCR with 2A linker

<400>  34

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His 
        115                 120                 125             


Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
    130                 135                 140                 


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
145                 150                 155                 160 


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val 
                165                 170                 175     


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
            180                 185                 190         


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
        195                 200                 205             


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp Val 
    210                 215                 220                 


Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
225                 230                 235                 240 


Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
                245                 250                 255     


Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser Arg Ala Lys Arg 
            260                 265                 270         


Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
        275                 280                 285             


Glu Glu Asn Pro Gly Pro Met Gly Ile Arg Leu Leu Cys Arg Val Ala 
    290                 295                 300                 


Phe Cys Phe Leu Ala Val Gly Leu Val Asp Val Lys Val Thr Gln Ser 
305                 310                 315                 320 


Ser Arg Tyr Leu Val Lys Arg Thr Gly Glu Lys Val Phe Leu Glu Cys 
                325                 330                 335     


Val Gln Asp Met Asp His Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro 
            340                 345                 350         


Gly Leu Gly Leu Arg Leu Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys 
        355                 360                 365             


Glu Lys Gly Asp Ile Pro Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys 
    370                 375                 380                 


Glu Arg Phe Ser Leu Ile Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser 
385                 390                 395                 400 


Met Tyr Leu Cys Ala Ser Ser Ser Gly Val Thr Gly Glu Leu Phe Phe 
                405                 410                 415     


Gly Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu Lys Asn Val Phe 
            420                 425                 430         


Pro Pro Glu Val Ala Val Phe Glu Pro Ser Glu Ala Glu Ile Ser His 
        435                 440                 445             


Thr Gln Lys Ala Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp 
    450                 455                 460                 


His Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly 
465                 470                 475                 480 


Val Cys Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp 
                485                 490                 495     


Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp 
            500                 505                 510         


Gln Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu 
        515                 520                 525             


Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys Pro Val Thr Gln 
    530                 535                 540                 


Ile Val Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly Phe Thr Ser 
545                 550                 555                 560 


Glu Ser Tyr Gln Gln Gly Val Leu Ser Ala Thr Ile Leu Tyr Glu Ile 
                565                 570                 575     


Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val Ser Ala Leu Val 
            580                 585                 590         


Leu Met Ala Met Val Lys Arg Lys Asp Ser Arg Gly 
        595                 600                 


<210>  35
<211>  624
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Double Myc-tagged Radium-1 scTCR with 2A linker

<400>  35

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg 
145                 150                 155                 160 


Asp Ser Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp 
                165                 170                 175     


Ser Gln Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr 
            180                 185                 190         


Asp Lys Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser 
        195                 200                 205             


Ala Val Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe 
    210                 215                 220                 


Asn Asn Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser 
225                 230                 235                 240 


Ser Cys Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn 
                245                 250                 255     


Leu Asn Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu 
            260                 265                 270         


Lys Val Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
        275                 280                 285             


Arg Ala Lys Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln 
    290                 295                 300                 


Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly Ile Arg Leu Leu 
305                 310                 315                 320 


Cys Arg Val Ala Phe Cys Phe Leu Ala Val Gly Leu Val Asp Val Lys 
                325                 330                 335     


Val Thr Gln Ser Ser Arg Tyr Leu Val Lys Arg Thr Gly Glu Lys Val 
            340                 345                 350         


Phe Leu Glu Cys Val Gln Asp Met Asp His Glu Asn Met Phe Trp Tyr 
        355                 360                 365             


Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu Ile Tyr Phe Ser Tyr Asp 
    370                 375                 380                 


Val Lys Met Lys Glu Lys Gly Asp Ile Pro Glu Gly Tyr Ser Val Ser 
385                 390                 395                 400 


Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile Leu Glu Ser Ala Ser Thr 
                405                 410                 415     


Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser Ser Ser Gly Val Thr Gly 
            420                 425                 430         


Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu 
        435                 440                 445             


Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro Ser Glu Ala 
    450                 455                 460                 


Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu Ala Thr Gly 
465                 470                 475                 480 


Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu 
                485                 490                 495     


Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro 
            500                 505                 510         


Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser 
        515                 520                 525             


Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln 
    530                 535                 540                 


Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys 
545                 550                 555                 560 


Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys 
                565                 570                 575     


Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser Ala Thr Ile 
            580                 585                 590         


Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val 
        595                 600                 605             


Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp Ser Arg Gly 
    610                 615                 620                 


<210>  36
<211>  624
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Double Myc-tagged cysteine-modified Radium-1 scTCR with 2A linker

<400>  36

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg 
145                 150                 155                 160 


Asp Ser Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp 
                165                 170                 175     


Ser Gln Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr 
            180                 185                 190         


Asp Lys Cys Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser 
        195                 200                 205             


Ala Val Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe 
    210                 215                 220                 


Asn Asn Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser 
225                 230                 235                 240 


Ser Cys Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn 
                245                 250                 255     


Leu Asn Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu 
            260                 265                 270         


Lys Val Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
        275                 280                 285             


Arg Ala Lys Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln 
    290                 295                 300                 


Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly Ile Arg Leu Leu 
305                 310                 315                 320 


Cys Arg Val Ala Phe Cys Phe Leu Ala Val Gly Leu Val Asp Val Lys 
                325                 330                 335     


Val Thr Gln Ser Ser Arg Tyr Leu Val Lys Arg Thr Gly Glu Lys Val 
            340                 345                 350         


Phe Leu Glu Cys Val Gln Asp Met Asp His Glu Asn Met Phe Trp Tyr 
        355                 360                 365             


Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu Ile Tyr Phe Ser Tyr Asp 
    370                 375                 380                 


Val Lys Met Lys Glu Lys Gly Asp Ile Pro Glu Gly Tyr Ser Val Ser 
385                 390                 395                 400 


Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile Leu Glu Ser Ala Ser Thr 
                405                 410                 415     


Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser Ser Ser Gly Val Thr Gly 
            420                 425                 430         


Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu 
        435                 440                 445             


Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro Ser Glu Ala 
    450                 455                 460                 


Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu Ala Thr Gly 
465                 470                 475                 480 


Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu 
                485                 490                 495     


Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro 
            500                 505                 510         


Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser 
        515                 520                 525             


Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln 
    530                 535                 540                 


Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys 
545                 550                 555                 560 


Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys 
                565                 570                 575     


Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser Ala Thr Ile 
            580                 585                 590         


Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val 
        595                 600                 605             


Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp Ser Arg Gly 
    610                 615                 620                 


<210>  37
<211>  545
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 scTCR with murinised constant regions and 2A linker

<400>  37

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro Ile 
        115                 120                 125             


Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser Gln 
    130                 135                 140                 


Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn Val 
145                 150                 155                 160 


Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Thr Val Leu 
                165                 170                 175     


Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp Ser 
            180                 185                 190         


Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn Ala 
        195                 200                 205             


Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu Lys 
    210                 215                 220                 


Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser Val Met 
225                 230                 235                 240 


Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu Met 
                245                 250                 255     


Thr Leu Arg Leu Trp Ser Ser Arg Ala Lys Arg Gly Ser Gly Ala Thr 
            260                 265                 270         


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
        275                 280                 285             


Pro Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala 
    290                 295                 300                 


Val Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val 
305                 310                 315                 320 


Lys Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp 
                325                 330                 335     


His Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg 
            340                 345                 350         


Leu Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile 
        355                 360                 365             


Pro Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu 
    370                 375                 380                 


Ile Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala 
385                 390                 395                 400 


Ser Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg 
                405                 410                 415     


Leu Thr Val Leu Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser 
            420                 425                 430         


Leu Phe Glu Pro Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr 
        435                 440                 445             


Leu Val Cys Leu Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser 
    450                 455                 460                 


Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro 
465                 470                 475                 480 


Gln Ala Tyr Lys Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu 
                485                 490                 495     


Arg Val Ser Ala Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys 
            500                 505                 510         


Gln Val Gln Phe His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly 
        515                 520                 525             


Ser Pro Lys Pro Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg 
    530                 535                 540                 


Ala 
545 


<210>  38
<211>  545
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Radium-1 scTCR with cysteine-modified murinised constant regions 
       and 2A linker

<400>  38

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro Ile 
        115                 120                 125             


Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser Gln 
    130                 135                 140                 


Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn Val 
145                 150                 155                 160 


Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Cys Val Leu 
                165                 170                 175     


Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp Ser 
            180                 185                 190         


Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn Ala 
        195                 200                 205             


Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu Lys 
    210                 215                 220                 


Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser Val Met 
225                 230                 235                 240 


Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu Met 
                245                 250                 255     


Thr Leu Arg Leu Trp Ser Ser Arg Ala Lys Arg Gly Ser Gly Ala Thr 
            260                 265                 270         


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
        275                 280                 285             


Pro Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala 
    290                 295                 300                 


Val Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val 
305                 310                 315                 320 


Lys Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp 
                325                 330                 335     


His Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg 
            340                 345                 350         


Leu Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile 
        355                 360                 365             


Pro Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu 
    370                 375                 380                 


Ile Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala 
385                 390                 395                 400 


Ser Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg 
                405                 410                 415     


Leu Thr Val Leu Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser 
            420                 425                 430         


Leu Phe Glu Pro Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr 
        435                 440                 445             


Leu Val Cys Leu Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser 
    450                 455                 460                 


Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro 
465                 470                 475                 480 


Gln Ala Tyr Lys Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu 
                485                 490                 495     


Arg Val Ser Ala Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys 
            500                 505                 510         


Gln Val Gln Phe His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly 
        515                 520                 525             


Ser Pro Lys Pro Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg 
    530                 535                 540                 


Ala 
545 


<210>  39
<211>  565
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Double Myc-tagged Radium-1 scTCR with murinised constant regions

<400>  39

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp 
145                 150                 155                 160 


Pro Arg Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser 
                165                 170                 175     


Gln Ile Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp 
            180                 185                 190         


Lys Thr Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala 
        195                 200                 205             


Ile Ala Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys 
    210                 215                 220                 


Glu Thr Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr 
225                 230                 235                 240 


Leu Thr Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn 
                245                 250                 255     


Leu Ser Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe 
            260                 265                 270         


Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser Arg Ala Lys Arg Gly 
        275                 280                 285             


Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu 
    290                 295                 300                 


Glu Asn Pro Gly Pro Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe 
305                 310                 315                 320 


Cys Phe Leu Ala Val Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser 
                325                 330                 335     


Arg Tyr Leu Val Lys Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val 
            340                 345                 350         


Gln Asp Met Asp His Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly 
        355                 360                 365             


Leu Gly Leu Arg Leu Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu 
    370                 375                 380                 


Lys Gly Asp Ile Pro Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu 
385                 390                 395                 400 


Arg Phe Ser Leu Ile Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met 
                405                 410                 415     


Tyr Leu Cys Ala Ser Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly 
            420                 425                 430         


Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu Arg Asn Val Thr Pro 
        435                 440                 445             


Pro Lys Val Ser Leu Phe Glu Pro Ser Lys Ala Glu Ile Ala Asn Lys 
    450                 455                 460                 


Gln Lys Ala Thr Leu Val Cys Leu Ala Arg Gly Phe Phe Pro Asp His 
465                 470                 475                 480 


Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val 
                485                 490                 495     


Ser Thr Asp Pro Gln Ala Tyr Lys Glu Ser Asn Tyr Ser Tyr Cys Leu 
            500                 505                 510         


Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp His Asn Pro Arg Asn 
        515                 520                 525             


His Phe Arg Cys Gln Val Gln Phe His Gly Leu Ser Glu Glu Asp Lys 
    530                 535                 540                 


Trp Pro Glu Gly Ser Pro Lys Pro Val Thr Gln Asn Ile Ser Ala Glu 
545                 550                 555                 560 


Ala Trp Gly Arg Ala 
                565 


<210>  40
<211>  565
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Double Myc-tagged Radium-1 scTCR with cysteine-modified murinised
       constant regions and 2A linker

<400>  40

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Glu Gln 
            20                  25                  30          


Lys Leu Ile Ser Glu Glu Asp Leu Gly Ile Gln Val Glu Gln Ser Pro 
        35                  40                  45              


Pro Asp Leu Ile Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn 
    50                  55                  60                  


Phe Ser Asp Ser Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp 
65                  70                  75                  80  


Gly Gln Leu Ile Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn 
                85                  90                  95      


Gly Arg Leu Ser Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu 
            100                 105                 110         


Tyr Ile Ser Ser Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala 
        115                 120                 125             


Val Asn Ala Gly Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met 
    130                 135                 140                 


Val Lys Pro Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp 
145                 150                 155                 160 


Pro Arg Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser 
                165                 170                 175     


Gln Ile Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp 
            180                 185                 190         


Lys Cys Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala 
        195                 200                 205             


Ile Ala Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys 
    210                 215                 220                 


Glu Thr Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr 
225                 230                 235                 240 


Leu Thr Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn 
                245                 250                 255     


Leu Ser Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe 
            260                 265                 270         


Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser Arg Ala Lys Arg Gly 
        275                 280                 285             


Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu 
    290                 295                 300                 


Glu Asn Pro Gly Pro Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe 
305                 310                 315                 320 


Cys Phe Leu Ala Val Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser 
                325                 330                 335     


Arg Tyr Leu Val Lys Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val 
            340                 345                 350         


Gln Asp Met Asp His Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly 
        355                 360                 365             


Leu Gly Leu Arg Leu Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu 
    370                 375                 380                 


Lys Gly Asp Ile Pro Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu 
385                 390                 395                 400 


Arg Phe Ser Leu Ile Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met 
                405                 410                 415     


Tyr Leu Cys Ala Ser Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly 
            420                 425                 430         


Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu Arg Asn Val Thr Pro 
        435                 440                 445             


Pro Lys Val Ser Leu Phe Glu Pro Ser Lys Ala Glu Ile Ala Asn Lys 
    450                 455                 460                 


Gln Lys Ala Thr Leu Val Cys Leu Ala Arg Gly Phe Phe Pro Asp His 
465                 470                 475                 480 


Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val His Ser Gly Val 
                485                 490                 495     


Cys Thr Asp Pro Gln Ala Tyr Lys Glu Ser Asn Tyr Ser Tyr Cys Leu 
            500                 505                 510         


Ser Ser Arg Leu Arg Val Ser Ala Thr Phe Trp His Asn Pro Arg Asn 
        515                 520                 525             


His Phe Arg Cys Gln Val Gln Phe His Gly Leu Ser Glu Glu Asp Lys 
    530                 535                 540                 


Trp Pro Glu Gly Ser Pro Lys Pro Val Thr Gln Asn Ile Ser Ala Glu 
545                 550                 555                 560 


Ala Trp Gly Arg Ala 
                565 


<210>  41
<211>  1815
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 33

<400>  41
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      120

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      180

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      240

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      300

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      360

acaaggttaa tggtcaaacc ccatatccag aaccctgacc ctgccgtgta ccagctgaga      420

gactctaaat ccagtgacaa gtctgtctgc ctattcaccg attttgattc tcaaacaaat      480

gtgtcacaaa gtaaggattc tgatgtgtat atcacagaca aaactgtgct agacatgagg      540

tctatggact tcaagagcaa cagtgctgtg gcctggagca acaaatctga ctttgcatgt      600

gcaaacgcct tcaacaacag cattattcca gaagacacct tcttccccag cccagaaagt      660

tcctgtgatg tcaagctggt cgagaaaagc tttgaaacag atacgaacct aaactttcaa      720

aacctgtcag tgattgggtt ccgaatcctc ctcctaaaag tggccgggtt taatctgctc      780

atgacgctgc ggctgtggtc cagcagagcc aagagaggca gcggcgccac caacttcagc      840

ctgctgaagc aggccggcga cgtggaagag aaccctggac caatgggaat caggctcctc      900

tgtcgtgtgg ccttttgttt cctggctgta ggcctcgtag atgtgaaagt aacccagagc      960

tcgagatatc tagtcaaaag gacgggagag aaagtttttc tggaatgtgt ccaggatatg     1020

gaccatgaaa atatgttctg gtatcgacaa gacccaggtc tggggctacg gctgatctat     1080

ttctcatatg atgttaaaat gaaagaaaaa ggagatattc ctgaggggta cagtgtctct     1140

agagagaaga aggagcgctt ctccctgatt ctggagtccg ccagcaccaa ccagacatct     1200

atgtacctct gtgccagcag ttctggagtc accggggagc tgttttttgg agaaggctct     1260

aggctgaccg tactggagga cctgaaaaac gtgttcccac ccgaggtcgc tgtgtttgag     1320

ccatcagaag cagagatctc ccacacccaa aaggccacac tggtgtgcct ggccacaggc     1380

ttctaccccg accacgtgga gctgagctgg tgggtgaatg ggaaggaggt gcacagtggg     1440

gtcagcacag acccgcagcc cctcaaggag cagcccgccc tcaatgactc cagatactgc     1500

ctgagcagcc gcctgagggt ctcggccacc ttctggcaga acccccgcaa ccacttccgc     1560

tgtcaagtcc agttctacgg gctctcggag aatgacgagt ggacccagga tagggccaaa     1620

cctgtcaccc agatcgtcag cgccgaggcc tggggtagag cagactgtgg cttcacctcc     1680

gagtcttacc agcaaggggt cctgtctgcc accatcctct atgagatctt gctagggaag     1740

gccaccttgt atgccgtgct ggtcagtgcc ctcgtgctga tggccatggt caagagaaag     1800

gattccagag gctag                                                      1815


<210>  42
<211>  1815
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 34

<400>  42
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      120

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      180

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      240

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      300

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      360

acaaggttaa tggtcaaacc ccatatccag aaccctgacc ctgccgtgta ccagctgaga      420

gactctaaat ccagtgacaa gtctgtctgc ctattcaccg attttgattc tcaaacaaat      480

gtgtcacaaa gtaaggattc tgatgtgtat atcacagaca aatgcgtgct agacatgagg      540

tctatggact tcaagagcaa cagtgctgtg gcctggagca acaaatctga ctttgcatgt      600

gcaaacgcct tcaacaacag cattattcca gaagacacct tcttccccag cccagaaagt      660

tcctgtgatg tcaagctggt cgagaaaagc tttgaaacag atacgaacct aaactttcaa      720

aacctgtcag tgattgggtt ccgaatcctc ctcctaaaag tggccgggtt taatctgctc      780

atgacgctgc ggctgtggtc cagcagagcc aagagaggca gcggcgccac caacttcagc      840

ctgctgaagc aggccggcga cgtggaagag aaccctggac caatgggaat caggctcctc      900

tgtcgtgtgg ccttttgttt cctggctgta ggcctcgtag atgtgaaagt aacccagagc      960

tcgagatatc tagtcaaaag gacgggagag aaagtttttc tggaatgtgt ccaggatatg     1020

gaccatgaaa atatgttctg gtatcgacaa gacccaggtc tggggctacg gctgatctat     1080

ttctcatatg atgttaaaat gaaagaaaaa ggagatattc ctgaggggta cagtgtctct     1140

agagagaaga aggagcgctt ctccctgatt ctggagtccg ccagcaccaa ccagacatct     1200

atgtacctct gtgccagcag ttctggagtc accggggagc tgttttttgg agaaggctct     1260

aggctgaccg tactggagga cctgaaaaac gtgttcccac ccgaggtcgc tgtgtttgag     1320

ccatcagaag cagagatctc ccacacccaa aaggccacac tggtgtgcct ggccacaggc     1380

ttctaccccg accacgtgga gctgagctgg tgggtgaatg ggaaggaggt gcacagtggg     1440

gtctgtacag acccgcagcc cctcaaggag cagcccgccc tcaatgactc cagatactgc     1500

ctgagcagcc gcctgagggt ctcggccacc ttctggcaga acccccgcaa ccacttccgc     1560

tgtcaagtcc agttctacgg gctctcggag aatgacgagt ggacccagga tagggccaaa     1620

cctgtcaccc agatcgtcag cgccgaggcc tggggtagag cagactgtgg cttcacctcc     1680

gagtcttacc agcaaggggt cctgtctgcc accatcctct atgagatctt gctagggaag     1740

gccaccttgt atgccgtgct ggtcagtgcc ctcgtgctga tggccatggt caagagaaag     1800

gattccagag gctag                                                      1815


<210>  43
<211>  1875
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 35

<400>  43
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

gagcagaagc tgatcagcga ggaggacctg gagcagaagc tgatcagcga ggaggacctg      120

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      180

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      240

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      300

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      360

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      420

acaaggttaa tggtcaaacc ccatatccag aaccctgacc ctgccgtgta ccagctgaga      480

gactctaaat ccagtgacaa gtctgtctgc ctattcaccg attttgattc tcaaacaaat      540

gtgtcacaaa gtaaggattc tgatgtgtat atcacagaca aaactgtgct agacatgagg      600

tctatggact tcaagagcaa cagtgctgtg gcctggagca acaaatctga ctttgcatgt      660

gcaaacgcct tcaacaacag cattattcca gaagacacct tcttccccag cccagaaagt      720

tcctgtgatg tcaagctggt cgagaaaagc tttgaaacag atacgaacct aaactttcaa      780

aacctgtcag tgattgggtt ccgaatcctc ctcctaaaag tggccgggtt taatctgctc      840

atgacgctgc ggctgtggtc cagcagagcc aagagaggca gcggcgccac caacttcagc      900

ctgctgaagc aggccggcga cgtggaagag aaccctggac caatgggaat caggctcctc      960

tgtcgtgtgg ccttttgttt cctggctgta ggcctcgtag atgtgaaagt aacccagagc     1020

tccagatatc tagtcaaaag gacgggagag aaagtttttc tggaatgtgt ccaggatatg     1080

gaccatgaaa atatgttctg gtatcgacaa gacccaggtc tggggctacg gctgatctat     1140

ttctcatatg atgttaaaat gaaagaaaaa ggagatattc ctgaggggta cagtgtctct     1200

agagagaaga aggagcgctt ctccctgatt ctggagtccg ccagcaccaa ccagacatct     1260

atgtacctct gtgccagcag ttctggagtc accggggagc tgttttttgg agaaggctct     1320

aggctgaccg tactggagga cctgaaaaac gtgttcccac ccgaggtcgc tgtgtttgag     1380

ccatcagaag cagagatctc ccacacccaa aaggccacac tggtgtgcct ggccacaggc     1440

ttctaccccg accacgtgga gctgagctgg tgggtgaatg ggaaggaggt gcacagtggg     1500

gtcagcacag acccgcagcc cctcaaggag cagcccgccc tcaatgactc cagatactgc     1560

ctgagcagcc gcctgagggt ctcggccacc ttctggcaga acccccgcaa ccacttccgc     1620

tgtcaagtcc agttctacgg gctctcggag aatgacgagt ggacccagga tagggccaaa     1680

cctgtcaccc agatcgtcag cgccgaggcc tggggtagag cagactgtgg cttcacctcc     1740

gagtcttacc agcaaggggt cctgtctgcc accatcctct atgagatctt gctagggaag     1800

gccaccttgt atgccgtgct ggtcagtgcc ctcgtgctga tggccatggt caagagaaag     1860

gattccagag gctag                                                      1875


<210>  44
<211>  1875
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 36

<400>  44
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

gagcagaagc tgatcagcga ggaggacctg gagcagaagc tgatcagcga ggaggacctg      120

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      180

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      240

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      300

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      360

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      420

acaaggttaa tggtcaaacc ccatatccag aaccctgacc ctgccgtgta ccagctgaga      480

gactctaaat ccagtgacaa gtctgtctgc ctattcaccg attttgattc tcaaacaaat      540

gtgtcacaaa gtaaggattc tgatgtgtat atcacagaca aatgcgtgct agacatgagg      600

tctatggact tcaagagcaa cagtgctgtg gcctggagca acaaatctga ctttgcatgt      660

gcaaacgcct tcaacaacag cattattcca gaagacacct tcttccccag cccagaaagt      720

tcctgtgatg tcaagctggt cgagaaaagc tttgaaacag atacgaacct aaactttcaa      780

aacctgtcag tgattgggtt ccgaatcctc ctcctaaaag tggccgggtt taatctgctc      840

atgacgctgc ggctgtggtc cagcagagcc aagagaggca gcggcgccac caacttcagc      900

ctgctgaagc aggccggcga cgtggaagag aaccctggac caatgggaat caggctcctc      960

tgtcgtgtgg ccttttgttt cctggctgta ggcctcgtag atgtgaaagt aacccagagc     1020

tcgagatatc tagtcaaaag gacgggagag aaagtttttc tggaatgtgt ccaggatatg     1080

gaccatgaaa atatgttctg gtatcgacaa gacccaggtc tggggctacg gctgatctat     1140

ttctcatatg atgttaaaat gaaagaaaaa ggagatattc ctgaggggta cagtgtctct     1200

agagagaaga aggagcgctt ctccctgatt ctggagtccg ccagcaccaa ccagacatct     1260

atgtacctct gtgccagcag ttctggagtc accggggagc tgttttttgg agaaggctct     1320

aggctgaccg tactggagga cctgaaaaac gtgttcccac ccgaggtcgc tgtgtttgag     1380

ccatcagaag cagagatctc ccacacccaa aaggccacac tggtgtgcct ggccacaggc     1440

ttctaccccg accacgtgga gctgagctgg tgggtgaatg ggaaggaggt gcacagtggg     1500

gtctgtacag acccgcagcc cctcaaggag cagcccgccc tcaatgactc cagatactgc     1560

ctgagcagcc gcctgagggt ctcggccacc ttctggcaga acccccgcaa ccacttccgc     1620

tgtcaagtcc agttctacgg gctctcggag aatgacgagt ggacccagga tagggccaaa     1680

cctgtcaccc agatcgtcag cgccgaggcc tggggtagag cagactgtgg cttcacctcc     1740

gagtcttacc agcaaggggt cctgtctgcc accatcctct atgagatctt gctagggaag     1800

gccaccttgt atgccgtgct ggtcagtgcc ctcgtgctga tggccatggt caagagaaag     1860

gattccagag gctag                                                      1875


<210>  45
<211>  1638
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 37

<400>  45
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      120

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      180

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      240

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      300

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      360

acaaggttaa tggtcaaacc catccagaac ccagaacctg ctgtgtacca gttaaaagat      420

cctcggtctc aggacagcac cctctgcctg ttcaccgact ttgactccca aatcaatgtg      480

ccgaaaacca tggaatctgg aacgttcatc actgacaaaa ctgtgctgga catgaaagct      540

atggattcca agagcaatgg ggccattgcc tggagcaacc agacaagctt cacctgccaa      600

gatatcttca aagagaccaa cgccacctac cccagttcag acgttccctg tgatgccacg      660

ttgactgaga aaagctttga aacagatatg aacctaaact ttcaaaacct gtcagttatg      720

ggactccgaa tcctcctgct gaaagtagcc ggatttaacc tgctcatgac gctgaggctg      780

tggtccagta gagccaagag aggcagcggc gccaccaact tcagcctgct gaagcaggcc      840

ggcgacgtgg aagagaaccc tggaccaatg ggaatcaggc tcctctgtcg tgtggccttt      900

tgtttcctgg ctgtaggcct cgtagatgtg aaagtaaccc agagctcgag atatctagtc      960

aaaaggacgg gagagaaagt ttttctggaa tgtgtccagg atatggacca tgaaaatatg     1020

ttctggtatc gacaagaccc aggtctgggg ctacggctga tctatttctc atatgatgtt     1080

aaaatgaaag aaaaaggaga tattcctgag gggtacagtg tctctagaga gaagaaggag     1140

cgcttctccc tgattctgga gtccgccagc accaaccaga catctatgta cctctgtgcc     1200

agcagttctg gagtcaccgg ggagctgttt tttggagaag gctctaggct gaccgtactg     1260

gaggatctga gaaatgtgac tccacccaag gtctccttgt ttgagccatc aaaagcagag     1320

attgcaaaca aacaaaaggc taccctcgtg tgcttggcca ggggcttctt ccctgaccac     1380

gtggagctga gctggtgggt gaatggcaag gaggtccaca gtggggtcag cacggaccct     1440

caggcctaca aggagagcaa ttatagctac tgcctgagca gccgcctgag ggtctctgct     1500

accttctggc acaatcctcg aaaccacttc cgctgccaag tgcagttcca tgggctttca     1560

gaggaggaca agtggccaga gggctcaccc aaacctgtca cacagaacat cagtgcagag     1620

gcctggggcc gagcatag                                                   1638


<210>  46
<211>  1638
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 38

<400>  46
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      120

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      180

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      240

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      300

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      360

acaaggttaa tggtcaaacc catccagaac ccagaacctg ctgtgtacca gttaaaagat      420

cctcggtctc aggacagcac cctctgcctg ttcaccgact ttgactccca aatcaatgtg      480

ccgaaaacca tggaatctgg aacgttcatc actgacaaat gcgtgctgga catgaaagct      540

atggattcca agagcaatgg ggccattgcc tggagcaacc agacaagctt cacctgccaa      600

gatatcttca aagagaccaa cgccacctac cccagttcag acgttccctg tgatgccacg      660

ttgactgaga aaagctttga aacagatatg aacctaaact ttcaaaacct gtcagttatg      720

ggactccgaa tcctcctgct gaaagtagcc ggatttaacc tgctcatgac gctgaggctg      780

tggtccagta gagccaagag aggcagcggc gccaccaact tcagcctgct gaagcaggcc      840

ggcgacgtgg aagagaaccc tggaccaatg ggaatcaggc tcctctgtcg tgtggccttt      900

tgtttcctgg ctgtaggcct cgtagatgtg aaagtaaccc agagctcgag atatctagtc      960

aaaaggacgg gagagaaagt ttttctggaa tgtgtccagg atatggacca tgaaaatatg     1020

ttctggtatc gacaagaccc aggtctgggg ctacggctga tctatttctc atatgatgtt     1080

aaaatgaaag aaaaaggaga tattcctgag gggtacagtg tctctagaga gaagaaggag     1140

cgcttctccc tgattctgga gtccgccagc accaaccaga catctatgta cctctgtgcc     1200

agcagttctg gagtcaccgg ggagctgttt tttggagaag gctctaggct gaccgtactg     1260

gaggatctga gaaatgtgac tccacccaag gtctccttgt ttgagccatc aaaagcagag     1320

attgcaaaca aacaaaaggc taccctcgtg tgcttggcca ggggcttctt ccctgaccac     1380

gtggagctga gctggtgggt gaatggcaag gaggtccaca gtggggtctg tacggaccct     1440

caggcctaca aggagagcaa ttatagctac tgcctgagca gccgcctgag ggtctctgct     1500

accttctggc acaatcctcg aaaccacttc cgctgccaag tgcagttcca tgggctttca     1560

gaggaggaca agtggccaga gggctcaccc aaacctgtca cacagaacat cagtgcagag     1620

gcctggggcc gagcatag                                                   1638


<210>  47
<211>  1698
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 39

<400>  47
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

gagcagaagc tgatcagcga ggaggacctg gagcagaagc tgatcagcga ggaggacctg      120

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      180

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      240

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      300

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      360

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      420

acaaggttaa tggtcaaacc catccagaac ccagaacctg ctgtgtacca gttaaaagat      480

cctcggtctc aggacagcac cctctgcctg ttcaccgact ttgactccca aatcaatgtg      540

ccgaaaacca tggaatctgg aacgttcatc actgacaaaa ctgtgctgga catgaaagct      600

atggattcca agagcaatgg ggccattgcc tggagcaacc agacaagctt cacctgccaa      660

gatatcttca aagagaccaa cgccacctac cccagttcag acgttccctg tgatgccacg      720

ttgactgaga aaagctttga aacagatatg aacctaaact ttcaaaacct gtcagttatg      780

ggactccgaa tcctcctgct gaaagtagcc ggatttaacc tgctcatgac gctgaggctg      840

tggtccagta gagccaagag aggcagcggc gccaccaact tcagcctgct gaagcaggcc      900

ggcgacgtgg aagagaaccc tggaccaatg ggaatcaggc tcctctgtcg tgtggccttt      960

tgtttcctgg ctgtaggcct cgtagatgtg aaagtaaccc agagctcgag atatctagtc     1020

aaaaggacgg gagagaaagt ttttctggaa tgtgtccagg atatggacca tgaaaatatg     1080

ttctggtatc gacaagaccc aggtctgggg ctacggctga tctatttctc atatgatgtt     1140

aaaatgaaag aaaaaggaga tattcctgag gggtacagtg tctctagaga gaagaaggag     1200

cgcttctccc tgattctgga gtccgccagc accaaccaga catctatgta cctctgtgcc     1260

agcagttctg gagtcaccgg ggagctgttt tttggagaag gctctaggct gaccgtactg     1320

gaggatctga gaaatgtgac tccacccaag gtctccttgt ttgagccatc aaaagcagag     1380

attgcaaaca aacaaaaggc taccctcgtg tgcttggcca ggggcttctt ccctgaccac     1440

gtggagctga gctggtgggt gaatggcaag gaggtccaca gtggggtcag cacggaccct     1500

caggcctaca aggagagcaa ttatagctac tgcctgagca gccgcctgag ggtctctgct     1560

accttctggc acaatcctcg aaaccacttc cgctgccaag tgcagttcca tgggctttca     1620

gaggaggaca agtggccaga gggctcaccc aaacctgtca cacagaacat cagtgcagag     1680

gcctggggcc gagcatag                                                   1698


<210>  48
<211>  1698
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 40

<400>  48
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

gagcagaagc tgatcagcga ggaggacctg gagcagaagc tgatcagcga ggaggacctg      120

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      180

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      240

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      300

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      360

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      420

acaaggttaa tggtcaaacc catccagaac ccagaacctg ctgtgtacca gttaaaagat      480

cctcggtctc aggacagcac cctctgcctg ttcaccgact ttgactccca aatcaatgtg      540

ccgaaaacca tggaatctgg aacgttcatc actgacaaat gcgtgctgga catgaaagct      600

atggattcca agagcaatgg ggccattgcc tggagcaacc agacaagctt cacctgccaa      660

gatatcttca aagagaccaa cgccacctac cccagttcag acgttccctg tgatgccacg      720

ttgactgaga aaagctttga aacagatatg aacctaaact ttcaaaacct gtcagttatg      780

ggactccgaa tcctcctgct gaaagtagcc ggatttaacc tgctcatgac gctgaggctg      840

tggtccagta gagccaagag aggcagcggc gccaccaact tcagcctgct gaagcaggcc      900

ggcgacgtgg aagagaaccc tggaccaatg ggaatcaggc tcctctgtcg tgtggccttt      960

tgtttcctgg ctgtaggcct cgtagatgtg aaagtaaccc agagctcgag atatctagtc     1020

aaaaggacgg gagagaaagt ttttctggaa tgtgtccagg atatggacca tgaaaatatg     1080

ttctggtatc gacaagaccc aggtctgggg ctacggctga tctatttctc atatgatgtt     1140

aaaatgaaag aaaaaggaga tattcctgag gggtacagtg tctctagaga gaagaaggag     1200

cgcttctccc tgattctgga gtccgccagc accaaccaga catctatgta cctctgtgcc     1260

agcagttctg gagtcaccgg ggagctgttt tttggagaag gctctaggct gaccgtactg     1320

gaggatctga gaaatgtgac tccacccaag gtctccttgt ttgagccatc aaaagcagag     1380

attgcaaaca aacaaaaggc taccctcgtg tgcttggcca ggggcttctt ccctgaccac     1440

gtggagctga gctggtgggt gaatggcaag gaggtccaca gtggggtctg tacggaccct     1500

caggcctaca aggagagcaa ttatagctac tgcctgagca gccgcctgag ggtctctgct     1560

accttctggc acaatcctcg aaaccacttc cgctgccaag tgcagttcca tgggctttca     1620

gaggaggaca agtggccaga gggctcaccc aaacctgtca cacagaacat cagtgcagag     1680

gcctggggcc gagcatag                                                   1698


<210>  49
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  49

Ser Leu Val Arg Leu Ser Ser Cys Val Pro Val Ala Leu Met Ser Ala 
1               5                   10                  15      


Met Thr Thr Ser Ser Ser Gln 
            20              


<210>  50
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  50

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg 
            20  


<210>  51
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  51

Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala Val 
1               5                   10                  15      


<210>  52
<211>  8
<212>  PRT
<213>  picornavirus


<220>
<221>  X
<222>  (2)..(2)
<223>  Val or Ile

<220>
<221>  X
<222>  (4)..(4)
<223>  Any amino acid

<400>  52

Asp Xaa Glu Xaa Asn Pro Gly Pro 
1               5               


<210>  53
<211>  10
<212>  DNA
<213>  Homo sapiens

<400>  53
aataaa                                                              10


<210>  54
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  54

Ile Leu Ala Lys Phe Leu His Trp Leu 
1               5                   


<210>  55
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  55

Lys Ser Leu Val Arg Leu Ser Ser Cys Val Pro Val Ala Leu Met Ser 
1               5                   10                  15      


Ala Met Thr 
            


<210>  56
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  56

Glu Ala Ala Gly Ile Gly Ile Leu Thr Val 
1               5                   10  


<210>  57
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Site-directed mutagenesis forward primer

<400>  57
gaggaccaga cccagaaggc ggagctcgtg gagac                                  35


<210>  58
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Site-directed mutagenesis reverse primer

<400>  58
gtctccacga gctccgcctt ctgggtctgg tcctc                                  35


<210>  59
<211>  15
<212>  PRT
<213>  Escherichia coli

<400>  59

Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His 
1               5                   10                  15  


<210>  60
<211>  95
<212>  PRT
<213>  Homo sapiens

<400>  60

His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys 
1               5                   10                  15      


Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr 
            20                  25                  30          


Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr 
        35                  40                  45              


Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala 
    50                  55                  60                  


Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser 
65                  70                  75                  80  


Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
                85                  90                  95  


<210>  61
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified truncated Radium-1 alpha-chain constant region

<400>  61

His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys 
1               5                   10                  15      


Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr 
            20                  25                  30          


Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys 
        35                  40                  45              


Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala 
    50                  55                  60                  


Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser 
65                  70                  75                  80  


Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
                85                  90                  95  


<210>  62
<211>  131
<212>  PRT
<213>  Homo sapiens

<400>  62

Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp Cys 
    130     


<210>  63
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified truncated Radium-1 beta-chain constant region

<400>  63

Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro 
1               5                   10                  15      


Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          


Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              


Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  


Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  


Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      


Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         


Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             


Ala Asp Cys 
    130     


<210>  64
<211>  222
<212>  PRT
<213>  Homo sapiens

<400>  64

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His 
        115                 120                 125             


Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
    130                 135                 140                 


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
145                 150                 155                 160 


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
                165                 170                 175     


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
            180                 185                 190         


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
        195                 200                 205             


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
    210                 215                 220         


<210>  65
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified truncated Radium-1 alpha-chain

<400>  65

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His 
        115                 120                 125             


Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
    130                 135                 140                 


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
145                 150                 155                 160 


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val 
                165                 170                 175     


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
            180                 185                 190         


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
        195                 200                 205             


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
    210                 215                 220         


<210>  66
<211>  262
<212>  PRT
<213>  Homo sapiens

<400>  66

Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala Val 
1               5                   10                  15      


Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
            20                  25                  30          


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
        35                  40                  45              


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
    50                  55                  60                  


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
65                  70                  75                  80  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
                85                  90                  95      


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
        115                 120                 125             


Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val 
    130                 135                 140                 


Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu 
145                 150                 155                 160 


Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp 
                165                 170                 175     


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln 
            180                 185                 190         


Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser 
        195                 200                 205             


Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His 
    210                 215                 220                 


Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp 
225                 230                 235                 240 


Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala 
                245                 250                 255     


Trp Gly Arg Ala Asp Cys 
            260         


<210>  67
<211>  262
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cysteine-modified truncated Radium-1 beta-chain

<400>  67

Met Gly Ile Arg Leu Leu Cys Arg Val Ala Phe Cys Phe Leu Ala Val 
1               5                   10                  15      


Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
            20                  25                  30          


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
        35                  40                  45              


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
    50                  55                  60                  


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
65                  70                  75                  80  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
                85                  90                  95      


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
            100                 105                 110         


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
        115                 120                 125             


Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val 
    130                 135                 140                 


Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu 
145                 150                 155                 160 


Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp 
                165                 170                 175     


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln 
            180                 185                 190         


Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser 
        195                 200                 205             


Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His 
    210                 215                 220                 


Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp 
225                 230                 235                 240 


Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala 
                245                 250                 255     


Trp Gly Arg Ala Asp Cys 
            260         


<210>  68
<211>  510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Soluble cysteine-modified Radium-1 scTCR with 2A linker

<400>  68

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His 
        115                 120                 125             


Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
    130                 135                 140                 


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
145                 150                 155                 160 


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val 
                165                 170                 175     


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
            180                 185                 190         


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
        195                 200                 205             


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Arg Ala 
    210                 215                 220                 


Lys Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly 
225                 230                 235                 240 


Asp Val Glu Glu Asn Pro Gly Pro Met Gly Ile Arg Leu Leu Cys Arg 
                245                 250                 255     


Val Ala Phe Cys Phe Leu Ala Val Gly Leu Val Asp Val Lys Val Thr 
            260                 265                 270         


Gln Ser Ser Arg Tyr Leu Val Lys Arg Thr Gly Glu Lys Val Phe Leu 
        275                 280                 285             


Glu Cys Val Gln Asp Met Asp His Glu Asn Met Phe Trp Tyr Arg Gln 
    290                 295                 300                 


Asp Pro Gly Leu Gly Leu Arg Leu Ile Tyr Phe Ser Tyr Asp Val Lys 
305                 310                 315                 320 


Met Lys Glu Lys Gly Asp Ile Pro Glu Gly Tyr Ser Val Ser Arg Glu 
                325                 330                 335     


Lys Lys Glu Arg Phe Ser Leu Ile Leu Glu Ser Ala Ser Thr Asn Gln 
            340                 345                 350         


Thr Ser Met Tyr Leu Cys Ala Ser Ser Ser Gly Val Thr Gly Glu Leu 
        355                 360                 365             


Phe Phe Gly Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu Lys Asn 
    370                 375                 380                 


Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro Ser Glu Ala Glu Ile 
385                 390                 395                 400 


Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr 
                405                 410                 415     


Pro Asp His Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val His 
            420                 425                 430         


Ser Gly Val Cys Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu 
        435                 440                 445             


Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala Thr 
    450                 455                 460                 


Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe Tyr 
465                 470                 475                 480 


Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys Pro Val 
                485                 490                 495     


Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys 
            500                 505                 510 


<210>  69
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Soluble cysteine-modified Radium-1 scTCR with C-terminal His-tag 
       and linker

<400>  69

Met Lys Arg Ile Leu Gly Ala Leu Leu Gly Leu Leu Ser Ala Gln Val 
1               5                   10                  15      


Cys Cys Val Arg Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile 
            20                  25                  30          


Leu Gln Glu Gly Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser 
        35                  40                  45              


Val Asn Asn Leu Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile 
    50                  55                  60                  


Asn Leu Phe Tyr Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser 
65                  70                  75                  80  


Ala Thr Thr Val Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser 
                85                  90                  95      


Ser Gln Thr Thr Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly 
            100                 105                 110         


Asn Met Leu Thr Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His 
        115                 120                 125             


Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
    130                 135                 140                 


Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
145                 150                 155                 160 


Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val 
                165                 170                 175     


Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
            180                 185                 190         


Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
        195                 200                 205             


Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Arg Ala 
    210                 215                 220                 


Lys Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly 
225                 230                 235                 240 


Asp Val Glu Glu Asn Pro Gly Pro Met Gly Ile Arg Leu Leu Cys Arg 
                245                 250                 255     


Val Ala Phe Cys Phe Leu Ala Val Gly Leu Val Asp Val Lys Val Thr 
            260                 265                 270         


Gln Ser Ser Arg Tyr Leu Val Lys Arg Thr Gly Glu Lys Val Phe Leu 
        275                 280                 285             


Glu Cys Val Gln Asp Met Asp His Glu Asn Met Phe Trp Tyr Arg Gln 
    290                 295                 300                 


Asp Pro Gly Leu Gly Leu Arg Leu Ile Tyr Phe Ser Tyr Asp Val Lys 
305                 310                 315                 320 


Met Lys Glu Lys Gly Asp Ile Pro Glu Gly Tyr Ser Val Ser Arg Glu 
                325                 330                 335     


Lys Lys Glu Arg Phe Ser Leu Ile Leu Glu Ser Ala Ser Thr Asn Gln 
            340                 345                 350         


Thr Ser Met Tyr Leu Cys Ala Ser Ser Ser Gly Val Thr Gly Glu Leu 
        355                 360                 365             


Phe Phe Gly Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu Lys Asn 
    370                 375                 380                 


Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro Ser Glu Ala Glu Ile 
385                 390                 395                 400 


Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu Ala Thr Gly Phe Tyr 
                405                 410                 415     


Pro Asp His Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val His 
            420                 425                 430         


Ser Gly Val Cys Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro Ala Leu 
        435                 440                 445             


Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala Thr 
    450                 455                 460                 


Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe Tyr 
465                 470                 475                 480 


Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys Pro Val 
                485                 490                 495     


Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly Gly 
            500                 505                 510         


Gly His His His His His His 
        515                 


<210>  70
<211>  1530
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 69

<400>  70
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      120

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      180

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      240

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      300

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      360

acaaggttaa tggtcaaacc ccatatccag aaccctgacc ctgccgtgta ccagctgaga      420

gactctaaat ccagtgacaa gtctgtctgc ctattcaccg attttgattc tcaaacaaat      480

gtgtcacaaa gtaaggattc tgatgtgtat atcacagaca aatgcgtgct agacatgagg      540

tctatggact tcaagagcaa cagtgctgtg gcctggagca acaaatctga ctttgcatgt      600

gcaaacgcct tcaacaacag cattattcca gaagacacct tcttccccag cccagaaagt      660

tcctgtagag ccaagagagg cagcggcgcc accaacttca gcctgctgaa gcaggccggc      720

gacgtggaag agaaccctgg accaatggga atcaggctcc tctgtcgtgt ggccttttgt      780

ttcctggctg taggcctcgt agatgtgaaa gtaacccaga gctcgagata tctagtcaaa      840

aggacgggag agaaagtttt tctggaatgt gtccaggata tggaccatga aaatatgttc      900

tggtatcgac aagacccagg tctggggcta cggctgatct atttctcata tgatgttaaa      960

atgaaagaaa aaggagatat tcctgagggg tacagtgtct ctagagagaa gaaggagcgc     1020

ttctccctga ttctggagtc cgccagcacc aaccagacat ctatgtacct ctgtgccagc     1080

agttctggag tcaccgggga gctgtttttt ggagaaggct ctaggctgac cgtactggag     1140

gacctgaaaa acgtgttccc acccgaggtc gctgtgtttg agccatcaga agcagagatc     1200

tcccacaccc aaaaggccac actggtgtgc ctggccacag gcttctaccc cgaccacgtg     1260

gagctgagct ggtgggtgaa tgggaaggag gtgcacagtg gggtctgtac agacccgcag     1320

cccctcaagg agcagcccgc cctcaatgac tccagatact gcctgagcag ccgcctgagg     1380

gtctcggcca ccttctggca gaacccccgc aaccacttcc gctgtcaagt ccagttctac     1440

gggctctcgg agaatgacga gtggacccag gatagggcca aacctgtcac ccagatcgtc     1500

agcgccgagg cctggggtag agcagactgt                                      1530


<210>  71
<211>  1557
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Encodes SEQ ID NO: 69

<400>  71
atgaagagga tattgggagc tctgctgggg ctcttgagtg cccaggtttg ctgtgtgaga       60

ggaatacaag tggagcagag tcctccagac ctgattctcc aggagggagc caattccacg      120

ctgcggtgca atttttctga ctctgtgaac aatttgcagt ggtttcatca aaacccttgg      180

ggacagctca tcaacctgtt ttacattccc tcagggacaa aacagaatgg aagattaagc      240

gccacgactg tcgctacgga acgctacagc ttattgtaca tttcctcttc ccagaccaca      300

gactcaggcg tttatttctg tgctgtgaat gcaggcaaca tgctcacctt tggaggggga      360

acaaggttaa tggtcaaacc ccatatccag aaccctgacc ctgccgtgta ccagctgaga      420

gactctaaat ccagtgacaa gtctgtctgc ctattcaccg attttgattc tcaaacaaat      480

gtgtcacaaa gtaaggattc tgatgtgtat atcacagaca aatgcgtgct agacatgagg      540

tctatggact tcaagagcaa cagtgctgtg gcctggagca acaaatctga ctttgcatgt      600

gcaaacgcct tcaacaacag cattattcca gaagacacct tcttccccag cccagaaagt      660

tcctgtagag ccaagagagg cagcggcgcc accaacttca gcctgctgaa gcaggccggc      720

gacgtggaag agaaccctgg accaatggga atcaggctcc tctgtcgtgt ggccttttgt      780

ttcctggctg taggcctcgt agatgtgaaa gtaacccaga gctcgagata tctagtcaaa      840

aggacgggag agaaagtttt tctggaatgt gtccaggata tggaccatga aaatatgttc      900

tggtatcgac aagacccagg tctggggcta cggctgatct atttctcata tgatgttaaa      960

atgaaagaaa aaggagatat tcctgagggg tacagtgtct ctagagagaa gaaggagcgc     1020

ttctccctga ttctggagtc cgccagcacc aaccagacat ctatgtacct ctgtgccagc     1080

agttctggag tcaccgggga gctgtttttt ggagaaggct ctaggctgac cgtactggag     1140

gacctgaaaa acgtgttccc acccgaggtc gctgtgtttg agccatcaga agcagagatc     1200

tcccacaccc aaaaggccac actggtgtgc ctggccacag gcttctaccc cgaccacgtg     1260

gagctgagct ggtgggtgaa tgggaaggag gtgcacagtg gggtctgtac agacccgcag     1320

cccctcaagg agcagcccgc cctcaatgac tccagatact gcctgagcag ccgcctgagg     1380

gtctcggcca ccttctggca gaacccccgc aaccacttcc gctgtcaagt ccagttctac     1440

gggctctcgg agaatgacga gtggacccag gatagggcca aacctgtcac ccagatcgtc     1500

agcgccgagg cctggggtag agcagactgt ggtggcggtc atcaccatca ccatcac        1557


<210>  72
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  72

Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile Leu Gln Glu Gly 
1               5                   10                  15      


Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser Val Asn Asn Leu 
            20                  25                  30          


Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile Asn Leu Phe Tyr 
        35                  40                  45              


Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser Ala Thr Thr Val 
    50                  55                  60                  


Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser Ser Gln Thr Thr 
65                  70                  75                  80  


Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly Asn Met Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro 
            100                 105         


<210>  73
<211>  202
<212>  PRT
<213>  Homo sapiens

<400>  73

Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile Leu Gln Glu Gly 
1               5                   10                  15      


Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser Val Asn Asn Leu 
            20                  25                  30          


Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile Asn Leu Phe Tyr 
        35                  40                  45              


Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser Ala Thr Thr Val 
    50                  55                  60                  


Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser Ser Gln Thr Thr 
65                  70                  75                  80  


Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly Asn Met Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His Ile Gln Asn Pro 
            100                 105                 110         


Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser Ser Asp Lys Ser 
        115                 120                 125             


Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn Val Ser Gln Ser 
    130                 135                 140                 


Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val Leu Asp Met Arg 
145                 150                 155                 160 


Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp Ser Asn Lys Ser 
                165                 170                 175     


Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile Ile Pro Glu Asp 
            180                 185                 190         


Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
        195                 200         


<210>  74
<211>  202
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature truncated cysteine-modified Radium-1 alpha-chain

<400>  74

Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile Leu Gln Glu Gly 
1               5                   10                  15      


Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser Val Asn Asn Leu 
            20                  25                  30          


Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile Asn Leu Phe Tyr 
        35                  40                  45              


Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser Ala Thr Thr Val 
    50                  55                  60                  


Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser Ser Gln Thr Thr 
65                  70                  75                  80  


Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly Asn Met Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His Ile Gln Asn Pro 
            100                 105                 110         


Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser Ser Asp Lys Ser 
        115                 120                 125             


Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn Val Ser Gln Ser 
    130                 135                 140                 


Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val Leu Asp Met Arg 
145                 150                 155                 160 


Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp Ser Asn Lys Ser 
                165                 170                 175     


Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile Ile Pro Glu Asp 
            180                 185                 190         


Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
        195                 200         


<210>  75
<211>  115
<212>  PRT
<213>  Homo sapiens

<400>  75

Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
1               5                   10                  15      


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
            20                  25                  30          


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
        35                  40                  45              


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
    50                  55                  60                  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
65                  70                  75                  80  


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
                85                  90                  95      


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
            100                 105                 110         


Thr Val Leu 
        115 


<210>  76
<211>  246
<212>  PRT
<213>  Homo sapiens

<400>  76

Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
1               5                   10                  15      


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
            20                  25                  30          


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
        35                  40                  45              


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
    50                  55                  60                  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
65                  70                  75                  80  


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
                85                  90                  95      


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
            100                 105                 110         


Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val 
        115                 120                 125             


Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu 
    130                 135                 140                 


Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp 
145                 150                 155                 160 


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln 
                165                 170                 175     


Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser 
            180                 185                 190         


Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His 
        195                 200                 205             


Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp 
    210                 215                 220                 


Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala 
225                 230                 235                 240 


Trp Gly Arg Ala Asp Cys 
                245     


<210>  77
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature truncated cysteine-modified Radium-1 beta-chain

<400>  77

Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
1               5                   10                  15      


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
            20                  25                  30          


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
        35                  40                  45              


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
    50                  55                  60                  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
65                  70                  75                  80  


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
                85                  90                  95      


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
            100                 105                 110         


Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val 
        115                 120                 125             


Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu 
    130                 135                 140                 


Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp 
145                 150                 155                 160 


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln 
                165                 170                 175     


Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser 
            180                 185                 190         


Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His 
        195                 200                 205             


Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp 
    210                 215                 220                 


Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala 
225                 230                 235                 240 


Trp Gly Arg Ala Asp Cys 
                245     


<210>  78
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature truncated cysteine-modified Radium-1 alpha-chain with 
       linker and His-tag

<400>  78

Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile Leu Gln Glu Gly 
1               5                   10                  15      


Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser Val Asn Asn Leu 
            20                  25                  30          


Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile Asn Leu Phe Tyr 
        35                  40                  45              


Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser Ala Thr Thr Val 
    50                  55                  60                  


Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser Ser Gln Thr Thr 
65                  70                  75                  80  


Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly Asn Met Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His Ile Gln Asn Pro 
            100                 105                 110         


Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser Ser Asp Lys Ser 
        115                 120                 125             


Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn Val Ser Gln Ser 
    130                 135                 140                 


Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val Leu Asp Met Arg 
145                 150                 155                 160 


Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp Ser Asn Lys Ser 
                165                 170                 175     


Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile Ile Pro Glu Asp 
            180                 185                 190         


Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Gly Gly Gly His His His 
        195                 200                 205             


His His His 
    210     


<210>  79
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature truncated cysteine-modified Radium-1 beta-chain with 
       linker and His-tag

<400>  79

Gly Leu Val Asp Val Lys Val Thr Gln Ser Ser Arg Tyr Leu Val Lys 
1               5                   10                  15      


Arg Thr Gly Glu Lys Val Phe Leu Glu Cys Val Gln Asp Met Asp His 
            20                  25                  30          


Glu Asn Met Phe Trp Tyr Arg Gln Asp Pro Gly Leu Gly Leu Arg Leu 
        35                  40                  45              


Ile Tyr Phe Ser Tyr Asp Val Lys Met Lys Glu Lys Gly Asp Ile Pro 
    50                  55                  60                  


Glu Gly Tyr Ser Val Ser Arg Glu Lys Lys Glu Arg Phe Ser Leu Ile 
65                  70                  75                  80  


Leu Glu Ser Ala Ser Thr Asn Gln Thr Ser Met Tyr Leu Cys Ala Ser 
                85                  90                  95      


Ser Ser Gly Val Thr Gly Glu Leu Phe Phe Gly Glu Gly Ser Arg Leu 
            100                 105                 110         


Thr Val Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val 
        115                 120                 125             


Phe Glu Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu 
    130                 135                 140                 


Val Cys Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp 
145                 150                 155                 160 


Trp Val Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln 
                165                 170                 175     


Pro Leu Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser 
            180                 185                 190         


Ser Arg Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His 
        195                 200                 205             


Phe Arg Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp 
    210                 215                 220                 


Thr Gln Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala 
225                 230                 235                 240 


Trp Gly Arg Ala Asp Cys Gly Gly Gly His His His His His His 
                245                 250                 255 


<210>  80
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MART-1 26-35 peptide analogue

<400>  80

Glu Leu Ala Gly Ile Gly Ile Leu Thr Val 
1               5                   10  


<210>  81
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature truncated Radium-1 alpha-chain with 2A peptide-derived 
       residues at C-terminus

<400>  81

Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile Leu Gln Glu Gly 
1               5                   10                  15      


Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser Val Asn Asn Leu 
            20                  25                  30          


Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile Asn Leu Phe Tyr 
        35                  40                  45              


Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser Ala Thr Thr Val 
    50                  55                  60                  


Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser Ser Gln Thr Thr 
65                  70                  75                  80  


Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly Asn Met Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His Ile Gln Asn Pro 
            100                 105                 110         


Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser Ser Asp Lys Ser 
        115                 120                 125             


Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn Val Ser Gln Ser 
    130                 135                 140                 


Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val Leu Asp Met Arg 
145                 150                 155                 160 


Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp Ser Asn Lys Ser 
                165                 170                 175     


Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile Ile Pro Glu Asp 
            180                 185                 190         


Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Arg Ala Lys Arg Gly Ser 
        195                 200                 205             


Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
    210                 215                 220                 


Asn Pro Gly 
225         


<210>  82
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature truncated cysteine-modified Radium-1 alpha-chain with 2A 
       peptide-derived residues at C-terminus

<400>  82

Gly Ile Gln Val Glu Gln Ser Pro Pro Asp Leu Ile Leu Gln Glu Gly 
1               5                   10                  15      


Ala Asn Ser Thr Leu Arg Cys Asn Phe Ser Asp Ser Val Asn Asn Leu 
            20                  25                  30          


Gln Trp Phe His Gln Asn Pro Trp Gly Gln Leu Ile Asn Leu Phe Tyr 
        35                  40                  45              


Ile Pro Ser Gly Thr Lys Gln Asn Gly Arg Leu Ser Ala Thr Thr Val 
    50                  55                  60                  


Ala Thr Glu Arg Tyr Ser Leu Leu Tyr Ile Ser Ser Ser Gln Thr Thr 
65                  70                  75                  80  


Asp Ser Gly Val Tyr Phe Cys Ala Val Asn Ala Gly Asn Met Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Arg Leu Met Val Lys Pro His Ile Gln Asn Pro 
            100                 105                 110         


Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser Ser Asp Lys Ser 
        115                 120                 125             


Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn Val Ser Gln Ser 
    130                 135                 140                 


Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val Leu Asp Met Arg 
145                 150                 155                 160 


Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp Ser Asn Lys Ser 
                165                 170                 175     


Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile Ile Pro Glu Asp 
            180                 185                 190         


Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Arg Ala Lys Arg Gly Ser 
        195                 200                 205             


Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu 
    210                 215                 220                 


Asn Pro Gly 
225         


