                         SEQUENCE LISTING

<110>  Whitehead Institute for Biomedical Research
 
<120>  INCREASING RNAi EFFICIENCY THROUGH SINGLE NUCLEOTIDE MISMATCHES

<130>  WIBR-157-WO1

<140>  PCT/US2017/29972
<141>  2017-04-27

<150>  US 62/328,589
<151>  2016-04-27

<160>  173   

<170>  PatentIn version 3.5

<210>  1
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  zebrafish Ago2 segment

<400>  1

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  2
<211>  873
<212>  PRT
<213>  Artificial

<220>
<223>  zebrafish Ago2 polypeptide (isoform 1)

<400>  2

Met Tyr Pro Ile Gly Ala Ala Gly Ala Thr Glu Leu Phe Gln Gly Arg 
1               5                   10                  15      


Pro Ser Ser Gly Ser Asp Val Ser Ala Pro Ala Ser Pro Pro Ala Pro 
            20                  25                  30          


Gln Glu Tyr Val Phe Lys Pro Pro Gln Arg Pro Asp Phe Gly Thr Met 
        35                  40                  45              


Gly Arg Thr Ile Lys Leu Gln Ala Asn Phe Phe Glu Met Glu Ile Pro 
    50                  55                  60                  


Lys Leu Glu Val Tyr His Tyr Glu Ile Asp Ile Lys Pro Glu Lys Cys 
65                  70                  75                  80  


Pro Arg Gly Val Asn Arg Glu Ile Val Glu His Met Val Gln His Phe 
                85                  90                  95      


Lys Thr Gln Ile Phe Gly Asp Arg Lys Pro Val Tyr Asp Gly Arg Lys 
            100                 105                 110         


Asn Leu Tyr Thr Ala Met Pro Leu Pro Ile Gly Arg Asp Lys Val Glu 
        115                 120                 125             


Leu Glu Val Thr Ile Pro Gly Glu Gly Lys Asp Arg Ser Phe Lys Val 
    130                 135                 140                 


Ala Ile Lys Trp Met Ser Cys Val Ser Leu Gln Ala Leu His Glu Ala 
145                 150                 155                 160 


Leu Ser Gly Arg Leu Pro Asn Ile Pro Phe Glu Thr Ile Gln Ala Leu 
                165                 170                 175     


Asp Val Val Met Arg His Leu Pro Ser Met Arg Tyr Thr Pro Val Gly 
            180                 185                 190         


Arg Ser Phe Phe Thr Pro Ser Glu Gly Cys Ser Asn Pro Leu Gly Gly 
        195                 200                 205             


Gly Arg Glu Val Trp Phe Gly Phe His Gln Ser Val Arg Pro Ser Leu 
    210                 215                 220                 


Trp Lys Met Met Leu Asn Ile Asp Val Ser Ala Thr Ala Phe Tyr Lys 
225                 230                 235                 240 


Ala Gln Pro Val Ile Glu Phe Met Cys Glu Val Leu Asp Phe Lys Ser 
                245                 250                 255     


Ile Glu Glu Gln Gln Lys Pro Leu Thr Asp Ser Gln Arg Val Lys Phe 
            260                 265                 270         


Thr Lys Glu Ile Lys Gly Leu Lys Val Glu Ile Thr His Cys Gly Gln 
        275                 280                 285             


Met Lys Arg Lys Tyr Arg Val Cys Asn Val Thr Arg Arg Pro Ala Ser 
    290                 295                 300                 


His Gln Thr Phe Pro Leu Gln Gln Glu Asn Gly Gln Thr Ile Glu Cys 
305                 310                 315                 320 


Thr Val Ala Gln Tyr Phe Lys Asp Lys Tyr Lys Leu Val Leu Arg Tyr 
                325                 330                 335     


Pro His Leu Pro Cys Leu Gln Val Gly Gln Glu Gln Lys His Thr Tyr 
            340                 345                 350         


Leu Pro Leu Glu Val Cys Asn Ile Val Ala Gly Gln Arg Cys Ile Lys 
        355                 360                 365             


Lys Leu Thr Asp Asn Gln Thr Ser Thr Met Ile Arg Ala Thr Ala Arg 
    370                 375                 380                 


Ser Ala Pro Asp Arg Gln Asp Glu Ile Ser Lys Leu Met Arg Ser Ala 
385                 390                 395                 400 


Asn Phe Asn Thr Asp Pro Tyr Val Arg Glu Phe Gly Val Met Val Arg 
                405                 410                 415     


Asp Asp Met Thr Glu Val Asn Gly Arg Val Leu Gln Ala Pro Ser Ile 
            420                 425                 430         


Leu Tyr Gly Gly Arg Asn Lys Ala Ile Ala Thr Pro Val Gln Gly Val 
        435                 440                 445             


Trp Asp Met Arg Asn Lys Gln Phe His Thr Gly Ile Glu Ile Lys Val 
    450                 455                 460                 


Trp Ala Ile Ala Cys Phe Ala Pro Gln Arg Gln Cys Thr Glu Leu Leu 
465                 470                 475                 480 


Leu Lys Ala Phe Thr Asp Gln Leu Arg Lys Ile Ser Arg Asp Ala Gly 
                485                 490                 495     


Met Pro Ile Gln Gly Gln Pro Cys Phe Cys Lys Tyr Ala Gln Gly Ala 
            500                 505                 510         


Asp Ser Val Glu Pro Met Phe Lys His Leu Lys Tyr Thr Tyr Gln Gly 
        515                 520                 525             


Leu Gln Leu Val Val Val Ile Leu Pro Gly Lys Thr Pro Val Tyr Ala 
    530                 535                 540                 


Glu Val Lys Arg Val Gly Asp Thr Val Leu Gly Met Ala Thr Gln Cys 
545                 550                 555                 560 


Val Gln Val Lys Asn Val Gln Lys Thr Thr Pro Gln Thr Leu Ser Asn 
                565                 570                 575     


Leu Cys Leu Lys Ile Asn Val Lys Leu Gly Gly Val Asn Asn Ile Leu 
            580                 585                 590         


Leu Pro Gln Gly Arg Pro Leu Val Phe Gln Gln Pro Val Ile Phe Leu 
        595                 600                 605             


Gly Ala Asp Val Thr His Pro Pro Ala Gly Asp Gly Lys Lys Pro Ser 
    610                 615                 620                 


Ile Ala Ala Val Val Gly Ser Met Asp Ala His Pro Ser Arg Tyr Cys 
625                 630                 635                 640 


Ala Thr Val Arg Val Gln Gln His Arg Gln Asp Ile Ile Gln Asp Leu 
                645                 650                 655     


Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe Tyr Lys Ser Thr Arg 
            660                 665                 670         


Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp Gly Ile Ser Glu Gly 
        675                 680                 685             


Gln Phe Asn Gln Val Leu Gln His Glu Leu Leu Ala Ile Arg Glu Ala 
    690                 695                 700                 


Cys Ile Lys Leu Glu Lys Asp Tyr Gln Pro Gly Ile Thr Phe Val Val 
705                 710                 715                 720 


Val Gln Lys Arg His His Thr Arg Leu Phe Cys Met Asp Arg Asn Glu 
                725                 730                 735     


Arg Val Gly Lys Ser Gly Asn Ile Pro Ala Gly Thr Thr Val Asp Thr 
            740                 745                 750         


Lys Ile Thr His Pro Phe Glu Phe Asp Phe Tyr Leu Cys Ser His Ala 
        755                 760                 765             


Gly Ile Gln Gly Thr Ser Arg Pro Ser His Tyr His Val Leu Trp Asp 
    770                 775                 780                 


Asp Asn His Phe Thr Ser Asp Glu Leu Gln Val Leu Thr Tyr Gln Leu 
785                 790                 795                 800 


Cys His Thr Tyr Val Arg Cys Thr Arg Ser Val Ser Ile Pro Ala Pro 
                805                 810                 815     


Ala Tyr Tyr Ala His Leu Val Ala Phe Arg Ala Arg Tyr His Leu Val 
            820                 825                 830         


Asp Lys Glu His Asp Ser Ala Glu Gly Ser His Thr Ser Gly Gln Ser 
        835                 840                 845             


Asn Gly Arg Asp Gln Gln Ala Leu Ala Lys Ala Val Gln Ile His Gln 
    850                 855                 860                 


Asp Thr Leu Arg Thr Met Tyr Phe Ala 
865                 870             


<210>  3
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Chimera N_for

<400>  3
gctacttgtt ctttttgcag gatcc                                             25


<210>  4
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  drAgo2 N_rev

<400>  4
cctcatggat ggcaagtgcc tcatgacaac atccagagcc                             40


<210>  5
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  hsAGO2 N_rev

<400>  5
cctcatagag ggcaaatgtc tcatgaccac gtccagggcc                             40


<210>  6
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  drAgo2 body_for

<400>  6
tggacggcta ccaaacatcc                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  hsAGO2 body_for

<400>  7
tgcctagcgt cccttttgag                                                   20


<210>  8
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Chimera body_rev

<400>  8
tggtttgtcc aaactcatca a                                                 21


<210>  9
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  drAgo2 D683A_sense

<400>  9
catcatctac tacagagccg gcatctctga aggcc                                  35


<210>  10
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  drAgo2 D683A_antisense

<400>  10
ggccttcaga gatgccggct ctgtagtaga tgatg                                  35


<210>  11
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  drAgo2 D651E_sense

<400>  11
cagcaccggc aggagatcat tcaggatctg                                        30


<210>  12
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  drAgo2 D651E_antisense

<400>  12
cagatcctga atgatctcct gccggtgctg                                        30


<210>  13
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  drAgo2 Y680F_sense

<400>  13
ccaacacgca tcatcttcta cagagacggc atc                                    33


<210>  14
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  drAgo2 Y680F_antisense

<400>  14
gatgccgtct ctgtagaaga tgatgcgtgt tgg                                    33


<210>  15
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  hsAGO2 D669A_sense

<400>  15
tcatcttcta ccgcgccggt gtctctgaag g                                      31


<210>  16
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  hsAGO2 D669A_antisense

<400>  16
ccttcagaga caccggcgcg gtagaagatg a                                      31


<210>  17
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  hsAGO2 E637D_sense

<400>  17
gcagcaccgg caggatatca tacaagacct g                                      31


<210>  18
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  hsAGO2 E637D_antisense

<400>  18
caggtcttgt atgatatcct gccggtgctg c                                      31


<210>  19
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  hsAGO2_F666Y_sense

<400>  19
cccacccgca tcatctatta ccgcgacggt gt                                     32


<210>  20
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  hsAGO2 F666Y_antisense

<400>  20
acaccgtcgc ggtaatagat gatgcgggtg gg                                     32


<210>  21
<211>  73
<212>  DNA
<213>  Artificial

<220>
<223>  Zeocin target_miR-430_for

<400>  21
actgactcga gcctctagaa ataagctacc ccaacttgat agcactttat aagctatagt       60

gagtcgtatt acg                                                          73


<210>  22
<211>  73
<212>  DNA
<213>  Artificial

<220>
<223>  Zeocin target_miR-430_rev

<400>  22
cgtaatacga ctcactatag cttataaagt gctatcaagt tggggtagct tatttctaga       60

ggctcgagtc agt                                                          73


<210>  23
<211>  73
<212>  DNA
<213>  Artificial

<220>
<223>  Zeocin_miR-430_10-11 mm_for

<400>  23
actgactcga gcctctagaa ataagctacc ccaacttctt agcactttat aagctatagt       60

gagtcgtatt acg                                                          73


<210>  24
<211>  73
<212>  DNA
<213>  Artificial

<220>
<223>  Zeocin miR-430_10-11 mm_rev

<400>  24
cgtaatacga ctcactatag cttataaagt gctaagaagt tggggtagct tatttctaga       60

ggctcgagtc agt                                                          73


<210>  25
<211>  73
<212>  DNA
<213>  Artificial

<220>
<223>  Zeocin_miR-430_G-G_for

<400>  25
actgactcga gcctctagaa ataagctacc ccaacttgat aggactttat aagctatagt       60

gagtcgtatt acg                                                          73


<210>  26
<211>  73
<212>  DNA
<213>  Artificial

<220>
<223>  Zeocin_miR-430_G-G_rev

<400>  26
cgtaatacga ctcactatag cttataaagt cctatcaagt tggggtagct tatttctaga       60

ggctcgagtc agt                                                          73


<210>  27
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Zeocin probe_for

<400>  27
attcaggatc atggccaagt tg                                                22


<210>  28
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  Zeocin probe_rev

<400>  28
ttctaatacg actcactata gggagaagga ggtttctaga ggctcgagtc agtc             54


<210>  29
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  GFP probe

<400>  29
gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat       60


<210>  30
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  168-nt target_for

<400>  30
gcgtaatacg actcactata gggtcacatc tcatctacct cc                          42


<210>  31
<211>  90
<212>  DNA
<213>  Artificial

<220>
<223>  168-nt target_miR-430_perfect_rev

<400>  31
cccatttaca tcgcgttgag tgtagaacgg ttgtataaaa ggtaaagtgc tatcaagttg       60

gggtagatcc agaggaattc attatcagtg                                        90


<210>  32
<211>  90
<212>  DNA
<213>  Artificial

<220>
<223>  168-nt target_miR-430_10-11 mm_rev

<400>  32
cccatttaca tcgcgttgag tgtagaacgg ttgtataaaa ggtaaagtgc taagaagttg       60

gggtagatcc agaggaattc attatcagtg                                        90


<210>  33
<211>  90
<212>  DNA
<213>  Artificial

<220>
<223>  168-nt target_miR-430_G-G_rev

<400>  33
cccatttaca tcgcgttgag tgtagaacgg ttgtataaaa ggtaaagtcc tatcaagttg       60

gggtagatcc agaggaattc attatcagtg                                        90


<210>  34
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  80-nt target_miR-430

<400>  34
ttgttgttgt tgttgttgtt aaagtgctat caagttgggg tagtgttgtt gttgttgttg       60

ttgttgttgt tgttgctccc tatagtgagt cgtattagaa                            100


<210>  35
<211>  100
<212>  DNA
<213>  Artificial

<220>
<223>  80-nt target_miR-430_G-G

<400>  35
ttgttgttgt tgttgttgtt aaagtcctat caagttgggg tagtgttgtt gttgttgttg       60

ttgttgttgt tgttgctccc tatagtgagt cgtattagaa                            100


<210>  36
<211>  74
<212>  DNA
<213>  Artificial

<220>
<223>  miR-430 capture

<400>  36
mumcmumumc mcmumcmcmg mcmamcmcma mcmamcmamg mcmamcmumu mamamcmcmu       60

mumamcmamc mamc                                                         74


<210>  37
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  miR-430 competitor

<400>  37
aaggttaagt gctgtgtggt gcggaggaag a                                      31


<210>  38
<211>  23
<212>  RNA
<213>  Artificial

<220>
<223>  miR-430b guide

<400>  38
aaagugcuau caaguugggg uag                                               23


<210>  39
<211>  22
<212>  RNA
<213>  Artificial

<220>
<223>  miR-430b passenger

<400>  39
acccuaacuu uagcaucuuu cu                                                22


<210>  40
<211>  42
<212>  RNA
<213>  Artificial

<220>
<223>  pre-miR-451 (ancestral)

<400>  40
aaaccguuac cauuacugag uuuaguaaug guaaggguuc ug                          42


<210>  41
<211>  42
<212>  RNA
<213>  Artificial

<220>
<223>  pre-miR-451 (amniote)

<400>  41
aaaccguuac cauuacugag uuuaguaaug guaacgguuc ug                          42


<210>  42
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  42

Pro Asn Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Phe Tyr Arg Asp 
        35                  40                  45              


Gly Val Ser Glu Gly Gln Phe Gln Gln Val Leu 
    50                  55                  


<210>  43
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  43

Pro Asn Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Phe Tyr Arg Asp 
        35                  40                  45              


Gly Val Ser Glu Gly Gln Phe Gln Gln Val Leu 
    50                  55                  


<210>  44
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  44

Pro Asn Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Phe Tyr Arg Asp 
        35                  40                  45              


Gly Val Ser Glu Gly Gln Phe Gln Gln Val Leu 
    50                  55                  


<210>  45
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  45

Pro Asn Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ser Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Phe Tyr Arg Asp 
        35                  40                  45              


Gly Val Ser Glu Gly Gln Phe Gln Gln Val Leu 
    50                  55                  


<210>  46
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  46

Pro Asn Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Phe Tyr Arg Asp 
        35                  40                  45              


Gly Val Ser Glu Gly Gln Phe Gln Gln Val Leu 
    50                  55                  


<210>  47
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  47

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  48
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  48

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Asn Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  49
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  49

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  50
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  50

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  51
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  51

Pro Asn Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ser Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Phe Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  52
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  52

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln His His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Gly Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Phe Tyr Arg Asp 
        35                  40                  45              


Gly Val Ser Glu Gly Gln Phe Gln Gln Val Leu 
    50                  55                  


<210>  53
<211>  21
<212>  RNA
<213>  Artificial

<220>
<223>  ancestral pre-miR-451 sequence

<400>  53
uuuaguaaug guaagggucu g                                                 21


<210>  54
<211>  20
<212>  RNA
<213>  Artificial

<220>
<223>  ancestral pre-miR-451 sequence

<400>  54
gagucauuac cauugccaaa                                                   20


<210>  55
<211>  22
<212>  RNA
<213>  Artificial

<220>
<223>  amniote pre-miR-451 sequence

<400>  55
uuuaguaaug guaacgguuc ug                                                22


<210>  56
<211>  20
<212>  RNA
<213>  Artificial

<220>
<223>  amniote pre-miR-451 sequence

<400>  56
gagucauuac cauugccaaa                                                   20


<210>  57
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  57

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ser Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Phe Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  58
<211>  58
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  58

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe His Gln Ala 
    50                  55              


<210>  59
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  59

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  60
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  60

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  61
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  61

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  62
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  62

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  63
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  63

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  64
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  64

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  65
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  65

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  66
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  66

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  67
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  67

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  68
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  68

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  69
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  69

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  70
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  70

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Ala Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  71
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  71

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  72
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  72

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  73
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  73

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  74
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  74

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  75
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  75

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Glu 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  76
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  76

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Asn Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  77
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  77

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Asn Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  78
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  78

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Asn Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  79
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  79

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Asn Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  80
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  80

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Asn Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  81
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  81

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  82
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  82

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  83
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  83

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  84
<211>  59
<212>  PRT
<213>  Artificial

<220>
<223>  Ago2 PIWI domain

<400>  84

Pro Ser Arg Tyr Cys Ala Thr Val Arg Val Gln Gln His Arg Gln Asp 
1               5                   10                  15      


Ile Ile Gln Asp Leu Ala Thr Met Val Arg Glu Leu Leu Ile Gln Phe 
            20                  25                  30          


Tyr Lys Ser Thr Arg Phe Lys Pro Thr Arg Ile Ile Tyr Tyr Arg Asp 
        35                  40                  45              


Gly Ile Ser Glu Gly Gln Phe Asn Gln Val Leu 
    50                  55                  


<210>  85
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  85
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  86
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  86
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  87
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  87
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  88
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  88
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  89
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  89
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  90
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  90
aaaccgttac cattactgag tttagtaatg gtaagggttc tc                          42


<210>  91
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  91
aaaccgttac cattactgag tttagtaatg gtaagggttc tc                          42


<210>  92
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  92
aaaccgttac cattactgag tttagtaatg gtaagggttc tc                          42


<210>  93
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  93
aaaccgttac cattactgag tttagtaatg gtaagggttc tc                          42


<210>  94
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  94
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  95
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  95
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  96
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  96
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  97
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  97
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  98
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  98
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  99
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  99
aaaccgttac cattactgag ttagtaatgg taacggttct c                           41


<210>  100
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  100
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  101
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  101
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  102
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  102
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  103
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  103
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  104
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  104
aaaccgttac cattactgag tttagtaatg gtgacggttc tc                          42


<210>  105
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  105
aaaccgttac cattactgag tttagtaatg gtgacggttc tc                          42


<210>  106
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  106
aaaccgttac cattactgag tttagtaatg gtgacggttc tc                          42


<210>  107
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  107
aaaccgttac cattactgag tttagtaatg gtgacggttc tc                          42


<210>  108
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  108
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  109
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  109
aaaccgttac cattacttag tttagtaatg gtaacggttc tc                          42


<210>  110
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  110
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  111
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  111
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  112
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  112
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  113
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  113
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  114
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  114
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  115
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  115
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  116
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  116
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  117
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  117
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  118
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  118
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  119
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  119
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  120
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  120
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  121
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  121
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  122
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  122
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  123
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  123
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  124
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  124
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  125
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  125
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  126
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  126
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  127
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  127
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  128
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  128
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  129
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  129
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  130
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  130
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  131
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  131
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  132
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  132
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  133
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  133
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  134
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  134
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  135
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  135
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  136
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  136
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  137
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  137
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  138
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  138
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  139
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  139
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  140
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  140
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  141
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  141
aaaccgttac cattactgag tttagtaatg gtaacggttc tc                          42


<210>  142
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  142
aaaccgttac cattactgtg tttagtaatg gtaagggttc tc                          42


<210>  143
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  143
aaaccgttac cattactgtg cttagtaatg gtaagggttc tc                          42


<210>  144
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  144
aaaccgttac cattactgtg tttagtaatg gtaacggttc tc                          42


<210>  145
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  145
aaaccgtttc aattactgag tttagtaatg gtaacggttc tc                          42


<210>  146
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  146
aaaccgttac cattactgag tttagtaatg gtaatggttc t                           41


<210>  147
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  147
aaaccgttac cattactgag tttagtaatg gtaacggttc t                           41


<210>  148
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  148
aaaccgttac cattactgag tttagtaatg gtaacggttc t                           41


<210>  149
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  149
aaaccgttac cattactgag tttagtaatg gtaacggttc t                           41


<210>  150
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  150
aaaccgttac cattactgag tttagtaatg gtaacggttc t                           41


<210>  151
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  151
aaaccgttac cattactgag tttagtaatg gtaacggttc t                           41


<210>  152
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  152
aaaccgttac cattactgag tttagtaatg gtaacggttc t                           41


<210>  153
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  153
aaaccgttac cattactgag tttagtaatg gtaacggttc t                           41


<210>  154
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  154
aaaccgttac cattactgag tttagtaatg gtaacggttc t                           41


<210>  155
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  155
aaaccgttac cattactgag tttagtaatg gtaatggttc t                           41


<210>  156
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  156
aaaccgttac cattactgac tttagtaatg gtaacggttc t                           41


<210>  157
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  157
aaaccgttac cattactgac tttagtaatg gtaacggttc t                           41


<210>  158
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  158
aaaccgttac cattactgag tttagtaatg gtaagggttc tg                          42


<210>  159
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  159
aaaccgttac cattactgaa tttagtaatg gtaagggttc tg                          42


<210>  160
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  160
aaaccgttac cattactgag tttagtaatg gtaagggttc                             40


<210>  161
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  161
aaaccgttac cattactgag tttagtaatg gtaagggttc tc                          42


<210>  162
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  162
aaaccgttac cattactgag tttagtaatg gtaagggttc tt                          42


<210>  163
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  163
aaaccgttac cattactgag tttagtaatg gtaagggttc t                           41


<210>  164
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  164
aaaccgttac cattactgag tttagtaatg gtaagggttc t                           41


<210>  165
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  165
aaaccgttac cattactgag tttagtaatg gtaagggttc t                           41


<210>  166
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  166
aaaccgttac cattactgag tttagtaatg gtaagggttc t                           41


<210>  167
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  167
aaaccgttac cattactgag tttagtaatg gtaagggttc ct                          42


<210>  168
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  168
aaaccgttac cattactgag tttagtaatg gtaagggttc tt                          42


<210>  169
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  169
aaaccgttac cattactgag tttagttatg gtaagggtta t                           41


<210>  170
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  170
aaaccgttac cattactgag tttagtaatg gtaagggttc ct                          42


<210>  171
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  171
aaaccgttac cattactgag tttagtaatg gtaagggttc tg                          42


<210>  172
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  172
aaaccgttac cattactgag tttagtaatg gtaagggttc tc                          42


<210>  173
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  pre-miR-451 DNA sequence

<400>  173
aaaccgttac cattactgag cttagtaatg gtaagggtta tg                          42


