                         SEQUENCE LISTING

<110>  Idea Orchard LLC
 
<120>  Engineered FHA Domain

<130>  27549

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  7935
<212>  DNA
<213>  Homo sapiens

<400>  1
ccagctcgcg ctgcccgggc gggcgccggc cgctggcgcc gctactgctg ccgcccccgg       60

ggcgcgagtc cgccgcccgc cgcccgggca cccggcgagg ggcgggggca gctccgaacc      120

ggccccagat ccttcccgct tccgcctcac gcttcccgga aagcttgtcc ctctccgccg      180

agctgctccg ggagccccgc cgcgccgagg gtatctccca gagccccagc tggtgtggcc      240

aggccccagg agtaggatgg ggctccccct acgagggccg gtggcagcca gaactgatac      300

agcccccctg gtctggggcc aggacgccag ctgaggaggg caggagtgtc tggagctatg      360

gctggtgcct cggtgaaagt ggcagtgagg gttcggccct ttaacgcccg tgagaccagc      420

caggatgcca agtgtgtggt cagcatgcag ggcaacacca cctccatcat caatcctaaa      480

cagagcaagg atgcccccaa aagcttcacc tttgactact cctactggtc acacacttcg      540

acggaggacc cccagtttgc atctcagcag caagtgtatc gggacattgg agaagagatg      600

ctgctccacg cctttgaagg ctacaacgtg tgcatctttg cctatgggca gaccggggct      660

gggaaatcct ataccatgat ggggcgacag gagccagggc agcagggcat cgtgccccag      720

ctctgtgagg acctcttctc tcgcgttagt gagaaccaga gtgctcagct atcctactct      780

gtggaggtga gctatatgga gatctactgt gagcgggtac gagacctctt gaaccccaag      840

agtcggggtt ctctgcgggt ccgggagcac cccatcctgg gcccgtacgt gcaggacctg      900

tccaaattgg ctgtgacctc ctacgcagac attgctgacc tcatggactg tggaaataaa      960

gcacggactg tggctgccac caacatgaat gagaccagca gccgttccca tgccgtcttt     1020

accatcgtct tcacacagcg ctgccatgac cagctcacgg ggctggactc ggagaaggtc     1080

agtaagatca gtttggtgga ccttgctggg agtgagcgag ccgactcctc aggggcccgg     1140

ggcatgcgcc tgaaggaagg agccaacatc aataagtccc tgactacact agggaaagtg     1200

atctcggccc ttgcagatat gcaatcaaag aagcgaaagt cggattttat cccctacagg     1260

gactctgtgc tcacctggct gctcaaggaa aatttggggg ggaactcacg cacagccatg     1320

attgcagccc tgagccctgc tgacatcaat tacgaggaga ctctcagcac cctcaggtat     1380

gctgaccgca ccaagcaaat ccgctgcaat gccatcatca acgaggaccc taatgcccgg     1440

ctgattagag agctgcagga ggaagtagcc cggctgcggg aactgctgat ggctcaggga     1500

ctgtcagcct ctgctctgga aggcctgaag acggaagaag ggagtgtcag aggcgccctg     1560

ccagctgtgt catctccccc agctccagtt tcaccctcat cacccaccac acataatggg     1620

gagctggagc cgtcattctc ccccaacacg gagtcccaga ttgggcctga ggaagccatg     1680

gagaggctgc aggagacaga gaagattata gctgagctga acgagacatg ggaggagaag     1740

ctacgcaaga cagaagccct gaggatggag agagaagcat tgctggctga gatgggggtg     1800

gccgtccggg aggatggggg aactgtgggc gtcttctctc caaagaagac tccccacctg     1860

gtgaacctga acgaagaccc tctgatgtct gagtgtctgc tctaccacat caaagatggc     1920

gtcaccaggg tcggccaagt agatatggac atcaagctga ccggacagtt cattcgggag     1980

caacactgtc tgttccggag catcccccag ccagatggag aagtggtggt cactctggag     2040

ccttgtgaag gagctgagac atatgtgaat gggaagcttg tgacggagcc gctggtgctg     2100

aagtcaggga ataggattgt gatgggcaag aaccacgttt tccgcttcaa ccacccggag     2160

caggcaaggc tggaacggga acgaggggtc cccccacccc caggaccgcc ctctgagcca     2220

gtcgactgga actttgccca gaaggaactg ctggagcagc aaggcatcga cataaagctg     2280

gaaatggaga agaggctgca ggatctggag aatcagtacc ggaaagaaaa ggaagaagcc     2340

gatcttctgc tggagcagca gcgactgtat gcagactcgg acagcgggga tgactctgac     2400

aagcgctctt gtgaagagag ctggaggctc atctcctcct tgcgggagca gctgccgccc     2460

accacggtcc agaccattgt caaacgctgt ggtctgccca gcagtggcaa gcgcagggcc     2520

cctcgcaggg tttatcagat cccccagcga cgcaggctgc agggcaaaga cccccgctgg     2580

gccaccatgg ctgacctgaa gatgcaggcg gtgaaggaga tctgctacga ggtggccctg     2640

gctgacttcc gccacgggcg ggctgagatt gaggccctgg ccgccctcaa gatgcgggag     2700

ctgtgtcgca cctatggcaa gccagacggc cccggagacg cctggagggc tgtggcccgg     2760

gatgtctggg acactgtagg cgaggaggaa ggaggtggag ctggcagtgg tggtggcagt     2820

gaggagggag cccgaggggc ggaggtggag gacctccggg cccacatcga caagctgacg     2880

gggattctgc aggaggtgaa gctgcagaac agcagcaagg accgggagct gcaggccctg     2940

cgggaccgca tgctccgcat ggagagggtc atccccctgg cccaggatca tgaggatgag     3000

aatgaagaag gtggtgaggt cccctgggcc ccgcctgaag gatcagaggc agcagaggag     3060

gcagccccca gtgaccgcat gccgtcagcc cggcccccct cgccaccact gtcaagctgg     3120

gagcgggtgt cacggctcat ggaggaggac cctgccttcc gtcgtggtcg tcttcgctgg     3180

ctcaagcagg agcagctacg gctgcaggga ctgcagggct ctgggggccg gggcgggggg     3240

ctgcgcaggc ccccagcccg ctttgtgccc cctcacgact gcaagctacg cttccccttc     3300

aagagcaacc cccagcaccg ggagtcttgg ccagggatgg ggagcgggga ggctccaact     3360

ccgctccaac cccctgagga ggtcactccc catccagcca cccctgcccg ccggcctccg     3420

agtccccgaa ggtcccacca tccccgcagg aactccctgg atggaggggg ccgatcccgg     3480

ggagcgggtt ctgcacagcc tgaaccccag cacttccagc ccaaaaagca caactcttat     3540

ccccagccac cccaacccta cccagcccag cggcccccag ggccccgcta ccccccatac     3600

actactcccc cacgaatgag acggcagcgt tctgcccctg acctcaagga gagtggggca     3660

gctgtgtgag tcccacatcc tgggcagagg gcctggtggg gccccttgct aggagaaggg     3720

aagacgcccg agacgctgct tccccagaag tgctggggca gggaggccca ggagatgaga     3780

gagaaggtcc gagtaggtga tagaagacaa gggggagacc gagccggagg ctgaggaaag     3840

gaagagggca cggagttgcc aggagcaaac caaagtgaag agagagatag gaagctgcct     3900

cggggccacc ccttgcaaag ggggtgtgtc ccacaaacgc tgctatgggt ggggtggggg     3960

gctggggtgc tgcgtagcca gtgtttgact ttcttttcaa gtgggggaaa gtgggagagg     4020

actgagagtg aggcaagttc tccccagccc ctgtccgtct gtctgtctgt ctgtggtggt     4080

ttctgtttct tgggaggcat ggtaggatca taagtcattc ccctcccctt ccaggcctcc     4140

tgctatattt gggggacctg actggtttgg ctggagtccc atgaggatgt gggcccttta     4200

ataaaggata gcaaacaggg agcttgtggc ctgtttgttt tgggttttca tggaggtgta     4260

ggttatataa ggcaatggca caggtcttaa gcatacttat cagtgaagta ttgtatgtgt     4320

gctctgtgca ggcaccaccc agatctggat ataagaatgt ttccatcttg tcttcctgaa     4380

cttcaccctc ctgtctcttc cttcagggtg cgcagcccga tcttttcccc gctttttttt     4440

tttgggagac agggtcttgc tttgttgccc aggctggagg tacagtcttg gctcactgca     4500

gcctccgcct cctgagtagc tgggattaca ggcatgtgcc accacgcccg gctcattact     4560

gttttttttg tagtgacgag gtttcaccat attggccagg ctggtctcga actcctgatg     4620

acctcaagtg atccgcccac cttggcctcc caaagtggtg ggattacagg tgtgagccac     4680

cgcgcccggc ctcccctgct ttcatgtttg cttacccagt gtctcagtct gtgccagcag     4740

caccactgtc tgttatggac aaagcacaga agcggggatg cgaggggagg tagagggacc     4800

gccagcctgt caatgcttaa ctggctgttg ctgacagata cagaaggatt tgtggggtac     4860

agaggagttc tgctttaagt cacctaactt acttgaaagg tctcccaagc agccagtttc     4920

tcttgatagt gaatgggttg ggcaagcata ctgcgcttac ctgattgttt tcatcctggt     4980

tgtgttacct gccttctggc aggctacatc attgttccca gtcgttattt tatttgtgtt     5040

aagactgttt gttggccagg cgcagtggtt cacgcctgta atgccagcac tgggaggccg     5100

aggcaggtgg atcacgaggt caggagatcg agaccatcct ggctatcatg gtgaaacccc     5160

atctctacta aaaataaaaa taaaaaaatt agccgggcat ggtggcgggg gcctgtagtc     5220

ccagctactc aggaggctga ggcaggagaa tggcatgaac ctgggaggcg gagcttgcag     5280

tgagctgaga ttgcgccact gcactccagc ctgggcgaca gagcgagact ccatttcaag     5340

aaaaattaaa aaactgtttg ttgcctgctt gttttaatgt tctggcttga ggcagcgagc     5400

ccttgactat gccacattgc caggattttg caggttagat tgtactacag cactgccttt     5460

ggcttgccag actctggagt ccccacattt tcatcctgtt ctcaggaaaa cactttgacc     5520

cacttgaagc tctgagctac tgcttcacag cttcctgggg tcagtctcca gccaaaacca     5580

tagatatccc aactgcagcc aaaccacggc tctgggcgaa ggaacgatta ggtttactct     5640

aggtttccac accctgatgc tcctgccttt actttgacac ctctgactgc caggttttca     5700

cagacctgtt gacagtgact caagatctgg aatgtaatgg atggtttggc aacagtgttt     5760

gcttgagcag tttaaaatct ggccagggag actcaatgtg agcaagaaaa tgatagaata     5820

ccaagttttc tggaggtcag agggagctga gttgagtgtt accgagaatg ctgtggggtt     5880

tgagatgaga aaaatagctt gctaatttaa aagacggatt atttttccct aaagaccttg     5940

acttatgtaa atgtatatta tccataattt taaaaatcca cttatggctg ggcacagaag     6000

ctcacacctg taatcccagc actttgggag gctgaggcag gaggatcagt tgagtccagg     6060

agttcgagac cagcctgggc aacatagtga aaccctgtat ctacaaaaca tagaaaaact     6120

agttgggtgt ggtggtgtgt acctgtagtt ccagctacta gggaggtagg aagatcacct     6180

gagcccatgg agattgaggc tgtggtgagc tgtgaaggca ccacagcact ctggcctggg     6240

caagagtggg actctctcaa aaaaaaaaaa aaaaaaaaat ccacatatcc agccttccaa     6300

gtcagtcttt aataatgtgc acgggccagg cacagtggct cacacccgta atcccaacac     6360

tttggaatat tagggtggga gcatcacttg aggccaggaa tttgagacca gcctgggcaa     6420

catagtgaga ctgccccccc catctctaca aaaaataaaa atcaaaaata atactgtggg     6480

caaactttac ttagatttta ttcagagtag tgcctgttta tttttcatct gtgctacctg     6540

tgccggcatt ccatatgcat ttgatttagt ctttaaagta ccccggtaaa ataggtcttt     6600

tgtctgcatt tttcagatgg gaagactgag gttttgttta tttattttta tttatttatt     6660

ttttgagacg gagtctcgct ctgttgccta ggctggagtg cgatggcaca atcttggctc     6720

actacaacct ccgcttcctg ggttcaagcg attctcctgc ctcagcctcc tgagtagcta     6780

ggattacagg cgcccacaac cgcacccggc taattttttg tatctttagt agagacaggg     6840

tttcactatg ttgtccaagc tggtttcgaa ttcctgacct caggtgatcc acccgcctct     6900

gcctcccaaa atgttgggat tacaggcgtg agccaccacg cccagcctat ttatttattt     6960

ttttagaatc aagatcttgc tatgttgccc aggctgaact cgaactcctg ggctcaagca     7020

atcctgcctc ccaagccaag cagctggtac tgcaagcatg tgccaccgtg cctggctcct     7080

gaagactgag gttggtttag attaatgagt tgcccaaatt cagacagttc ttaagtggca     7140

aagttaggat tccaacccag gtcaacataa ctaaagccca aaccccatct cacccctcta     7200

ggccatcaag tattggctgg gtgcggtggt ttatgtctgt aatcccagca ctttgggagg     7260

cggaggcggg aggactgttt gagctcagga gtttgagacc agcctgggca acataacaag     7320

accctgtctc tacaaaaata tataaattag ccgggtgtac tggctcacac ctgtagctac     7380

ttgggaggct gaggtgggag gatcgcttga gcctgggagg tcaaggctgc ggtgacctgt     7440

gattgcacca ccaggagacc ctgtcttaaa aatgtatata taaaaataaa aagaacatca     7500

gacatcacca tcacctcgct tagaatccat ggggccttct cctcaaattc aatcttccca     7560

ggcatttcat gaacttgcgt ctagtttctg ttccctctca aattgcccag gctgtcctgt     7620

gagtgacagc agctatttcg tgggcctcct ctggaatcat gagaagtcac ccaaacaatc     7680

tcagttttct agctcactcc gtcttgacat ttctacactg tcatccttgg ttttcttgga     7740

aattaatttg cttttcttca ttgtctttct ttggagctgc tttccttttg ttggttacta     7800

ttttattttt agcttctcac accataccga catatgttgg ttattctttt agacatgttt     7860

tttgttgttg ttgtcacctg gaacttttgt atcttgaata aatttgggga tcaaataaaa     7920

aaaaaaaaaa aaaaa                                                      7935


<210>  2
<211>  350
<212>  DNA
<213>  artificial sequence

<220>
<223>  Circular permutant of the 2G1L FHA domain

<400>  2
aaatttacca tgggcctgat gagcgaatgt ctgctgtatc atattaaaga tggtgttacc       60

cgtgttggtc aggtggatat ggatattaaa ctgacaggcc agtttattcg tgaacagcat      120

tgtctgtttc gtagcattcc gcagccggat ggtgaagttg ttgttaccct ggaaccgtgt      180

gaaggtgcag aaacctatgt taatggtaaa ctggttaccg aaccgctggt tctgaaaagc      240

ggtaatcgta ttgttatggg caaaaaccat gtgtttcgct ttaatcatac cggcaccggt      300

agcacaccgc atctggttaa tctgaatgaa gatccggcgg ccgcaattaa                 350


<210>  3
<211>  350
<212>  DNA
<213>  artificial sequence

<220>
<223>  Circular Permutant of 2G1L

<400>  3
aaatttacca tgggcgcaga aacctatgtg aatggtaaac tggttaccga accgctggtt       60

ctgaaaagcg gtaatcgtat tgttatgggc aaaaaccatg tgtttcgctt taatcatacc      120

ggcaccggta gcacaccgca tctggttaat ctgaatgaag atccgctgat gagcgaatgt      180

ctgctgtatc atattaaaga tggtgttacc cgtgttggtc aggtggatat ggatattaaa      240

ctgacaggcc agtttattcg tgaacagcat tgtctgtttc gtagcattcc gcagccggat      300

ggtgaagttg ttgttaccct ggaaccgtgt gaaggtgcgg ccgcaattaa                 350


<210>  4
<211>  350
<212>  DNA
<213>  artificial sequence

<220>
<223>  Circular permutant of 2G1L

<400>  4
aaatttacca tgggcattcg tgaacagcat tgtctgtttc gtagcattcc gcagccggat       60

ggtgaagttg ttgttaccct ggaaccgtgt gaaggtgcag aaacctatgt taatggtaaa      120

ctggttaccg aaccgctggt tctgaaaagc ggtaatcgta ttgttatggg caaaaaccat      180

gtgtttcgct ttaatcatac cggcaccggt agcacaccgc atctggttaa tctgaatgaa      240

gatccgctga tgagcgaatg tctgctgtat catattaaag atggtgttac ccgtgttggt      300

caggtggata tggatattaa actgacaggc cagtttgcgg ccgcaattaa                 350


<210>  5
<211>  57
<212>  DNA
<213>  artificial sequence

<220>
<223>  Mutagenesis primer of 2G1L and circular permutants of 2G1L


<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (38)..(39)
<223>  n is a, c, g, or t

<400>  5
ggttccaggg taacaacasn nsnnsnnsnn snnsnnsnna tgctacgaaa cagacaa          57


<210>  6
<211>  51
<212>  DNA
<213>  artificial sequence

<220>
<223>  Mutagenesis primer for 2G1L and circular permutant of 2G1L


<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<400>  6
accagatgcg gtgtgctasn nsnnsnnsnn snntgattaa agcgaaacac a                51


<210>  7
<211>  102
<212>  PRT
<213>  homo sapien

<400>  7

Thr Pro His Leu Val Asn Leu Asn Glu Asp Pro Leu Met Ser Glu Cys 
1               5                   10                  15      


Leu Leu Tyr His Ile Lys Asp Gly Val Thr Arg Val Gly Gln Val Asp 
            20                  25                  30          


Met Asp Ile Lys Leu Thr Gly Gln Phe Ile Arg Glu Gln His Cys Leu 
        35                  40                  45              


Phe Arg Ser Ile Pro Gln Pro Asp Gly Glu Val Val Val Thr Leu Glu 
    50                  55                  60                  


Pro Cys Glu Gly Ala Glu Thr Tyr Val Asn Gly Lys Leu Val Thr Glu 
65                  70                  75                  80  


Pro Leu Val Leu Lys Ser Gly Asn Arg Ile Val Met Gly Lys Asn His 
                85                  90                  95      


Val Phe Arg Phe Asn His 
            100         


<210>  8
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  Circular permutation of 2G1L

<400>  8

Leu Met Ser Glu Cys Leu Leu Tyr His Ile Lys Asp Gly Val Thr Arg 
1               5                   10                  15      


Val Gly Gln Val Asp Met Asp Ile Lys Leu Thr Gly Gln Phe Ile Arg 
            20                  25                  30          


Glu Gln His Cys Leu Phe Arg Ser Ile Pro Gln Pro Asp Gly Glu Val 
        35                  40                  45              


Val Val Thr Leu Glu Pro Cys Glu Gly Ala Glu Thr Tyr Val Asn Gly 
    50                  55                  60                  


Lys Leu Val Thr Glu Pro Leu Val Leu Lys Ser Gly Asn Arg Ile Val 
65                  70                  75                  80  


Met Gly Lys Asn His Val Phe Arg Phe Asn His Thr Gly Thr Gly Ser 
                85                  90                  95      


Thr Pro His Leu Val Asn Leu Asn Glu Asp Pro 
            100                 105         


<210>  9
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  Circular permutation of 2G1L

<400>  9

Ala Glu Thr Tyr Val Asn Gly Lys Leu Val Thr Glu Pro Leu Val Leu 
1               5                   10                  15      


Lys Ser Gly Asn Arg Ile Val Met Gly Lys Asn His Val Phe Arg Phe 
            20                  25                  30          


Asn His Thr Gly Thr Gly Ser Thr Pro His Leu Val Asn Leu Asn Glu 
        35                  40                  45              


Asp Pro Leu Met Ser Glu Cys Leu Leu Tyr His Ile Lys Asp Gly Val 
    50                  55                  60                  


Thr Arg Val Gly Gln Val Asp Met Asp Ile Lys Leu Thr Gly Gln Phe 
65                  70                  75                  80  


Ile Arg Glu Gln His Cys Leu Phe Arg Ser Ile Pro Gln Pro Asp Gly 
                85                  90                  95      


Glu Val Val Val Thr Leu Glu Pro Cys Glu Gly 
            100                 105         


<210>  10
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Circular Permutant of 2G1L

<400>  10

Ile Arg Glu Gln His Cys Leu Phe Arg Ser Ile Pro Gln Pro Asp Gly 
1               5                   10                  15      


Glu Val Val Val Thr Leu Glu Pro Cys Glu Gly Ala Glu Thr Tyr Val 
            20                  25                  30          


Asn Gly Lys Leu Val Thr Glu Pro Leu Val Leu Lys Ser Gly Asn Arg 
        35                  40                  45              


Ile Val Met Gly Lys Asn His Val Phe Arg Phe Asn His Thr Gly Thr 
    50                  55                  60                  


Gly Ser Thr Pro His Leu Val Asn Leu Asn Glu Asp Pro Leu Met Ser 
65                  70                  75                  80  


Glu Cys Leu Leu Tyr His Ile Lys Asp Gly Val Thr Arg Val Gly Gln 
                85                  90                  95      


Val Asp Met Asp Ile Lys Leu Thr Gly Gln Phe 
            100                 105         


