                         SEQUENCE LISTING

<110>  Danmarks Tekniske Universitet
 
<120>  High throughput in vivo protease inhibitor selection platform

<130>  P2066PC00

<150>  EP16167213.4
<151>  2016-04-27

<160>  80    

<170>  PatentIn version 3.5

<210>  1
<211>  1287
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (97)..(1287)
<223>  wtTetA gene encoding wild type (unmodified) tetracycline 
       resistance protein (tetR)

<400>  1
cggggcgatc gccgctagcg ttgacagctt atcatcgata agctttaatg cggtagttta       60

tcacagttaa attgctaacg cagtcaggca ccgtgt atg aaa tct aac aat gcg        114
                                        Met Lys Ser Asn Asn Ala           
                                        1               5                 

ctc atc gtc atc ctc ggc acc gtc acc ctg gat gct gta ggc ata ggc        162
Leu Ile Val Ile Leu Gly Thr Val Thr Leu Asp Ala Val Gly Ile Gly           
            10                  15                  20                    

ttg gtt atg ccg gta ctg ccg ggc ctc ttg cgg gat atc gtc cat tcc        210
Leu Val Met Pro Val Leu Pro Gly Leu Leu Arg Asp Ile Val His Ser           
        25                  30                  35                        

gac agc atc gcc agt cac tat ggc gtg ctg cta gcg cta tat gcg ttg        258
Asp Ser Ile Ala Ser His Tyr Gly Val Leu Leu Ala Leu Tyr Ala Leu           
    40                  45                  50                            

atg caa ttt cta tgc gca ccc gtt ctc gga gca ctg tcc gac cgc ttt        306
Met Gln Phe Leu Cys Ala Pro Val Leu Gly Ala Leu Ser Asp Arg Phe           
55                  60                  65                  70            

ggc cgc cgc cca gtc ctg ctc gct tcg cta ctt gga gcc act atc gac        354
Gly Arg Arg Pro Val Leu Leu Ala Ser Leu Leu Gly Ala Thr Ile Asp           
                75                  80                  85                

tac gcg atc atg gcg acc aca ccc gtc ctg tgg atc ctc tac gcc gga        402
Tyr Ala Ile Met Ala Thr Thr Pro Val Leu Trp Ile Leu Tyr Ala Gly           
            90                  95                  100                   

cgc atc gtg gcc ggc atc acc ggc gcc aca ggt gcg gtt gct ggc gcc        450
Arg Ile Val Ala Gly Ile Thr Gly Ala Thr Gly Ala Val Ala Gly Ala           
        105                 110                 115                       

tat atc gcc gac atc acc gat ggg gaa gat cgg gct cgc cac ttc ggg        498
Tyr Ile Ala Asp Ile Thr Asp Gly Glu Asp Arg Ala Arg His Phe Gly           
    120                 125                 130                           

ctc atg agc gct tgt ttc ggc gtg ggt atg gtg gca ggc ccc gtg gcc        546
Leu Met Ser Ala Cys Phe Gly Val Gly Met Val Ala Gly Pro Val Ala           
135                 140                 145                 150           

ggg gga ctg ttg ggc gcc atc tcc ttg cat gca cca ttc ctt gcg gcg        594
Gly Gly Leu Leu Gly Ala Ile Ser Leu His Ala Pro Phe Leu Ala Ala           
                155                 160                 165               

gcg gtg ctc aac ggc ctc aac cta cta ctg ggc tgc ttc cta atg cag        642
Ala Val Leu Asn Gly Leu Asn Leu Leu Leu Gly Cys Phe Leu Met Gln           
            170                 175                 180                   

gag tcg cat aag gga gag cgt cga acg atg ccc ttg aga gcc ttc aac        690
Glu Ser His Lys Gly Glu Arg Arg Thr Met Pro Leu Arg Ala Phe Asn           
        185                 190                 195                       

cca gtc agc tcc ttc cgg tgg gcg cgg ggc atg act atc gtc gcc gca        738
Pro Val Ser Ser Phe Arg Trp Ala Arg Gly Met Thr Ile Val Ala Ala           
    200                 205                 210                           

ctt atg act gtc ttc ttt atc atg caa ctc gta gga cag gtg ccg gca        786
Leu Met Thr Val Phe Phe Ile Met Gln Leu Val Gly Gln Val Pro Ala           
215                 220                 225                 230           

gcg ctc tgg gtc att ttc ggc gag gac cgc ttt cgc tgg agc gcg acg        834
Ala Leu Trp Val Ile Phe Gly Glu Asp Arg Phe Arg Trp Ser Ala Thr           
                235                 240                 245               

atg atc ggc ctg tcg ctt gcg gta ttc gga atc ttg cac gcc ctc gct        882
Met Ile Gly Leu Ser Leu Ala Val Phe Gly Ile Leu His Ala Leu Ala           
            250                 255                 260                   

caa gcc ttc gtc act ggt ccc gcc acc aaa cgt ttc ggc gag aag cag        930
Gln Ala Phe Val Thr Gly Pro Ala Thr Lys Arg Phe Gly Glu Lys Gln           
        265                 270                 275                       

gcc att atc gcc ggc atg gcg gcc gac gcg ctg ggc tac gtc ttg ctg        978
Ala Ile Ile Ala Gly Met Ala Ala Asp Ala Leu Gly Tyr Val Leu Leu           
    280                 285                 290                           

gcg ttc gcg acg cga ggc tgg atg gcc ttc ccc att atg att ctt ctc       1026
Ala Phe Ala Thr Arg Gly Trp Met Ala Phe Pro Ile Met Ile Leu Leu           
295                 300                 305                 310           

gct tcc ggc ggc atc ggg atg ccc gcg ttg cag gcc atg ctg tcc agg       1074
Ala Ser Gly Gly Ile Gly Met Pro Ala Leu Gln Ala Met Leu Ser Arg           
                315                 320                 325               

cag gta gat gac gac cat cag gga cag ctt caa gga tcg ctc gcg gct       1122
Gln Val Asp Asp Asp His Gln Gly Gln Leu Gln Gly Ser Leu Ala Ala           
            330                 335                 340                   

ctt acc agc cta act tcg atc act gga ccg ctg atc gtc acg gcg att       1170
Leu Thr Ser Leu Thr Ser Ile Thr Gly Pro Leu Ile Val Thr Ala Ile           
        345                 350                 355                       

tat gcc gcc tcg gcg agc aca tgg aac ggg ttg gca tgg att gta ggc       1218
Tyr Ala Ala Ser Ala Ser Thr Trp Asn Gly Leu Ala Trp Ile Val Gly           
    360                 365                 370                           

gcc gcc cta tac ctt gtc tgc ctc ccc gcg ttg cgt cgc ggt gca tgg       1266
Ala Ala Leu Tyr Leu Val Cys Leu Pro Ala Leu Arg Arg Gly Ala Trp           
375                 380                 385                 390           

agc cgg gcc acc tcg acc tga                                           1287
Ser Arg Ala Thr Ser Thr                                                   
                395                                                       


<210>  2
<211>  396
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Lys Ser Asn Asn Ala Leu Ile Val Ile Leu Gly Thr Val Thr Leu 
1               5                   10                  15      


Asp Ala Val Gly Ile Gly Leu Val Met Pro Val Leu Pro Gly Leu Leu 
            20                  25                  30          


Arg Asp Ile Val His Ser Asp Ser Ile Ala Ser His Tyr Gly Val Leu 
        35                  40                  45              


Leu Ala Leu Tyr Ala Leu Met Gln Phe Leu Cys Ala Pro Val Leu Gly 
    50                  55                  60                  


Ala Leu Ser Asp Arg Phe Gly Arg Arg Pro Val Leu Leu Ala Ser Leu 
65                  70                  75                  80  


Leu Gly Ala Thr Ile Asp Tyr Ala Ile Met Ala Thr Thr Pro Val Leu 
                85                  90                  95      


Trp Ile Leu Tyr Ala Gly Arg Ile Val Ala Gly Ile Thr Gly Ala Thr 
            100                 105                 110         


Gly Ala Val Ala Gly Ala Tyr Ile Ala Asp Ile Thr Asp Gly Glu Asp 
        115                 120                 125             


Arg Ala Arg His Phe Gly Leu Met Ser Ala Cys Phe Gly Val Gly Met 
    130                 135                 140                 


Val Ala Gly Pro Val Ala Gly Gly Leu Leu Gly Ala Ile Ser Leu His 
145                 150                 155                 160 


Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu Asn Leu Leu Leu 
                165                 170                 175     


Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu Arg Arg Thr Met 
            180                 185                 190         


Pro Leu Arg Ala Phe Asn Pro Val Ser Ser Phe Arg Trp Ala Arg Gly 
        195                 200                 205             


Met Thr Ile Val Ala Ala Leu Met Thr Val Phe Phe Ile Met Gln Leu 
    210                 215                 220                 


Val Gly Gln Val Pro Ala Ala Leu Trp Val Ile Phe Gly Glu Asp Arg 
225                 230                 235                 240 


Phe Arg Trp Ser Ala Thr Met Ile Gly Leu Ser Leu Ala Val Phe Gly 
                245                 250                 255     


Ile Leu His Ala Leu Ala Gln Ala Phe Val Thr Gly Pro Ala Thr Lys 
            260                 265                 270         


Arg Phe Gly Glu Lys Gln Ala Ile Ile Ala Gly Met Ala Ala Asp Ala 
        275                 280                 285             


Leu Gly Tyr Val Leu Leu Ala Phe Ala Thr Arg Gly Trp Met Ala Phe 
    290                 295                 300                 


Pro Ile Met Ile Leu Leu Ala Ser Gly Gly Ile Gly Met Pro Ala Leu 
305                 310                 315                 320 


Gln Ala Met Leu Ser Arg Gln Val Asp Asp Asp His Gln Gly Gln Leu 
                325                 330                 335     


Gln Gly Ser Leu Ala Ala Leu Thr Ser Leu Thr Ser Ile Thr Gly Pro 
            340                 345                 350         


Leu Ile Val Thr Ala Ile Tyr Ala Ala Ser Ala Ser Thr Trp Asn Gly 
        355                 360                 365             


Leu Ala Trp Ile Val Gly Ala Ala Leu Tyr Leu Val Cys Leu Pro Ala 
    370                 375                 380                 


Leu Arg Arg Gly Ala Trp Ser Arg Ala Thr Ser Thr 
385                 390                 395     


<210>  3
<211>  795
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(795)
<223>  wtKan gene encoding wild type (unmodified) kanamycin resistance 
       protein

<400>  3
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa ctg cag gac gag gca        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala           
    50                  55                  60                            

gcg cgg cta tcg tgg ctg gcc acg acg ggc gtt cct tgc gca gct gtg        240
Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val           
65                  70                  75                  80            

ctc gac gtt gtc act gaa gcg gga agg gac tgg ctg cta ttg ggc gaa        288
Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu           
                85                  90                  95                

gtg ccg ggg cag gat ctc ctg tca tct cac ctt gct cct gcc gag aaa        336
Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys           
            100                 105                 110                   

gta tcc atc atg gct gat gca atg cgg cgg ctg cat acg ctt gat ccg        384
Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro           
        115                 120                 125                       

gct acc tgc cca ttc gac cac caa gcg aaa cat cgc atc gag cga gca        432
Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala           
    130                 135                 140                           

cgt act cgg atg gaa gcc ggt ctt gtc gat cag gat gat ctg gac gaa        480
Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu           
145                 150                 155                 160           

gag cat cag ggg ctc gcg cca gcc gaa ctg ttc gcc agg ctc aag gcg        528
Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala           
                165                 170                 175               

cgc atg ccc gac ggc gag gat ctc gtc gtg acc cat ggc gat gcc tgc        576
Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys           
            180                 185                 190                   

ttg ccg aat atc atg gtg gaa aat ggc cgc ttt tct gga ttc atc gac        624
Leu Pro Asn Ile Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile Asp           
        195                 200                 205                       

tgt ggc cgg ctg ggt gtg gcg gac cgc tat cag gac ata gcg ttg gct        672
Cys Gly Arg Leu Gly Val Ala Asp Arg Tyr Gln Asp Ile Ala Leu Ala           
    210                 215                 220                           

acc cgt gat att gct gaa gag ctt ggc ggc gaa tgg gct gac cgc ttc        720
Thr Arg Asp Ile Ala Glu Glu Leu Gly Gly Glu Trp Ala Asp Arg Phe           
225                 230                 235                 240           

ctc gtg ctt tac ggt atc gcc gct ccc gat tcg cag cgc atc gcc ttc        768
Leu Val Leu Tyr Gly Ile Ala Ala Pro Asp Ser Gln Arg Ile Ala Phe           
                245                 250                 255               

tat cgc ctt ctt gac gag ttc ttc tga                                    795
Tyr Arg Leu Leu Asp Glu Phe Phe                                           
            260                                                           


<210>  4
<211>  264
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu 
                85                  90                  95      


Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys 
            100                 105                 110         


Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro 
        115                 120                 125             


Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala 
    130                 135                 140                 


Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu 
145                 150                 155                 160 


Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala 
                165                 170                 175     


Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys 
            180                 185                 190         


Leu Pro Asn Ile Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile Asp 
        195                 200                 205             


Cys Gly Arg Leu Gly Val Ala Asp Arg Tyr Gln Asp Ile Ala Leu Ala 
    210                 215                 220                 


Thr Arg Asp Ile Ala Glu Glu Leu Gly Gly Glu Trp Ala Asp Arg Phe 
225                 230                 235                 240 


Leu Val Leu Tyr Gly Ile Ala Ala Pro Asp Ser Gln Arg Ile Ala Phe 
                245                 250                 255     


Tyr Arg Leu Leu Asp Glu Phe Phe 
            260                 


<210>  5
<211>  637
<212>  DNA
<213>  Human rhinovirus


<220>
<221>  CDS
<222>  (83)..(637)
<223>  HRP gene encoding Human Rhinovirus protease

<400>  5
ttgattattt gcacggcgtc acacttttac agctagctca gtcctaggta ttatgctagc       60

agagaaagag gagaaatact ag atg ggt ccg gaa gaa gaa ttt ggt cgt agc       112
                         Met Gly Pro Glu Glu Glu Phe Gly Arg Ser          
                         1               5                   10           

att ctg aaa aac aat acc tgc gtt att acc acc gat aac ggc aaa ttt        160
Ile Leu Lys Asn Asn Thr Cys Val Ile Thr Thr Asp Asn Gly Lys Phe           
                15                  20                  25                

acc ggt ctg ggt att tat gat cgt acc ctg att att ccg acc cat gca        208
Thr Gly Leu Gly Ile Tyr Asp Arg Thr Leu Ile Ile Pro Thr His Ala           
            30                  35                  40                    

gat ccg ggt cgt gaa gtt cag gtt aat ggt att cat acc aaa gtg ctg        256
Asp Pro Gly Arg Glu Val Gln Val Asn Gly Ile His Thr Lys Val Leu           
        45                  50                  55                        

gat agc tat gat ctg tat aat cgt gat ggt gtg aaa ctg gaa atc acc        304
Asp Ser Tyr Asp Leu Tyr Asn Arg Asp Gly Val Lys Leu Glu Ile Thr           
    60                  65                  70                            

gtt att cag ctg gat cgc aat gaa aaa ttt cgc gat atc cgc aaa tat        352
Val Ile Gln Leu Asp Arg Asn Glu Lys Phe Arg Asp Ile Arg Lys Tyr           
75                  80                  85                  90            

atc ccg gaa acc gaa gat gat tat ccg gaa tgt aat ctg gca ctg agc        400
Ile Pro Glu Thr Glu Asp Asp Tyr Pro Glu Cys Asn Leu Ala Leu Ser           
                95                  100                 105               

gca aat cag gtt gaa ccg acc att atc aaa gtt ggt gat gtt gtg agc        448
Ala Asn Gln Val Glu Pro Thr Ile Ile Lys Val Gly Asp Val Val Ser           
            110                 115                 120                   

tat ggc aat att ctg ctg agc ggt aat cag acc gca cgt atg ctg aaa        496
Tyr Gly Asn Ile Leu Leu Ser Gly Asn Gln Thr Ala Arg Met Leu Lys           
        125                 130                 135                       

tac aat tat ccg acc aaa agc ggt tat tgt ggt ggt gtt ctg tat aaa        544
Tyr Asn Tyr Pro Thr Lys Ser Gly Tyr Cys Gly Gly Val Leu Tyr Lys           
    140                 145                 150                           

atc ggt cag att ctg ggc att cat gtt ggt ggt aat ggt cgt gat ggc        592
Ile Gly Gln Ile Leu Gly Ile His Val Gly Gly Asn Gly Arg Asp Gly           
155                 160                 165                 170           

ttt agc gca atg ctg ctg cgt agc tat ttt acc gat acc cag taa            637
Phe Ser Ala Met Leu Leu Arg Ser Tyr Phe Thr Asp Thr Gln                   
                175                 180                                   


<210>  6
<211>  184
<212>  PRT
<213>  Human rhinovirus

<400>  6

Met Gly Pro Glu Glu Glu Phe Gly Arg Ser Ile Leu Lys Asn Asn Thr 
1               5                   10                  15      


Cys Val Ile Thr Thr Asp Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr 
            20                  25                  30          


Asp Arg Thr Leu Ile Ile Pro Thr His Ala Asp Pro Gly Arg Glu Val 
        35                  40                  45              


Gln Val Asn Gly Ile His Thr Lys Val Leu Asp Ser Tyr Asp Leu Tyr 
    50                  55                  60                  


Asn Arg Asp Gly Val Lys Leu Glu Ile Thr Val Ile Gln Leu Asp Arg 
65                  70                  75                  80  


Asn Glu Lys Phe Arg Asp Ile Arg Lys Tyr Ile Pro Glu Thr Glu Asp 
                85                  90                  95      


Asp Tyr Pro Glu Cys Asn Leu Ala Leu Ser Ala Asn Gln Val Glu Pro 
            100                 105                 110         


Thr Ile Ile Lys Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu 
        115                 120                 125             


Ser Gly Asn Gln Thr Ala Arg Met Leu Lys Tyr Asn Tyr Pro Thr Lys 
    130                 135                 140                 


Ser Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Ile Leu Gly 
145                 150                 155                 160 


Ile His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ala Met Leu Leu 
                165                 170                 175     


Arg Ser Tyr Phe Thr Asp Thr Gln 
            180                 


<210>  7
<211>  35
<212>  DNA
<213>  Synthetic


<220>
<221>  promoter
<222>  (1)..(35)
<223>  JM23101 promoter synthetic sequence

<400>  7
tttacagcta gctcagtcct aggtattatg ctagc                                  35


<210>  8
<211>  7
<212>  PRT
<213>  synthetic


<220>
<221>  PEPTIDE
<222>  (1)..(7)
<223>  Recognition site amino acid sequence cleavable by Human rhino 
       protease 

Xaa = Threonine or valine or alanine

<400>  8

Xaa Leu Phe Gln Gly Pro Val 
1               5           


<210>  9
<211>  9
<212>  PRT
<213>  Synthetic


<220>
<221>  PEPTIDE
<222>  (1)..(9)
<223>  Recognition site amino acid sequence cleavable by Human rhino 
       protease

<400>  9

Glu Val Leu Phe Gln Gly Pro Val Tyr 
1               5                   


<210>  10
<211>  424
<212>  DNA
<213>  Human immunodeficiency virus


<220>
<221>  CDS
<222>  (92)..(424)
<223>  HIV protease gene encoding human immunodeficiency virus protease

<400>  10
gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccg tttttttggg       60

ctagctacta gagaaagagg agaaatacta g atg gcc gat aga caa gga act         112
                                   Met Ala Asp Arg Gln Gly Thr            
                                   1               5                      

gta tcc ttt aac ttc cct cag atc act ctt tgg caa cga ccc ctc gtc        160
Val Ser Phe Asn Phe Pro Gln Ile Thr Leu Trp Gln Arg Pro Leu Val           
        10                  15                  20                        

aca ata aag ata ggg ggg caa cta aag gaa gct cta tta gat aca gga        208
Thr Ile Lys Ile Gly Gly Gln Leu Lys Glu Ala Leu Leu Asp Thr Gly           
    25                  30                  35                            

gca gat gat aca gta tta gaa gaa atg agt ttg cca gga aga tgg aaa        256
Ala Asp Asp Thr Val Leu Glu Glu Met Ser Leu Pro Gly Arg Trp Lys           
40                  45                  50                  55            

cca aaa atg ata ggg gga att gga ggt ttt atc aaa gta aga cag tat        304
Pro Lys Met Ile Gly Gly Ile Gly Gly Phe Ile Lys Val Arg Gln Tyr           
                60                  65                  70                

gat cag ata ctc ata gaa atc tgt gga cat aaa gct ata ggt aca gta        352
Asp Gln Ile Leu Ile Glu Ile Cys Gly His Lys Ala Ile Gly Thr Val           
            75                  80                  85                    

tta gta gga cct aca cct gtc aac ata att gga aga aat ctg ttg act        400
Leu Val Gly Pro Thr Pro Val Asn Ile Ile Gly Arg Asn Leu Leu Thr           
        90                  95                  100                       

cag att ggt tgc act tta aat ttt                                        424
Gln Ile Gly Cys Thr Leu Asn Phe                                           
    105                 110                                               


<210>  11
<211>  111
<212>  PRT
<213>  Human immunodeficiency virus

<400>  11

Met Ala Asp Arg Gln Gly Thr Val Ser Phe Asn Phe Pro Gln Ile Thr 
1               5                   10                  15      


Leu Trp Gln Arg Pro Leu Val Thr Ile Lys Ile Gly Gly Gln Leu Lys 
            20                  25                  30          


Glu Ala Leu Leu Asp Thr Gly Ala Asp Asp Thr Val Leu Glu Glu Met 
        35                  40                  45              


Ser Leu Pro Gly Arg Trp Lys Pro Lys Met Ile Gly Gly Ile Gly Gly 
    50                  55                  60                  


Phe Ile Lys Val Arg Gln Tyr Asp Gln Ile Leu Ile Glu Ile Cys Gly 
65                  70                  75                  80  


His Lys Ala Ile Gly Thr Val Leu Val Gly Pro Thr Pro Val Asn Ile 
                85                  90                  95      


Ile Gly Arg Asn Leu Leu Thr Gln Ile Gly Cys Thr Leu Asn Phe 
            100                 105                 110     


<210>  12
<211>  7
<212>  PRT
<213>  Synthetic


<220>
<221>  PEPTIDE
<222>  (1)..(7)
<223>  Recognition site amino acid sequence cleavable by Human 
       Immunodeficiency Virus protease

<400>  12

Ser Gln Asn Tyr Pro Ile Val 
1               5           


<210>  13
<211>  4
<212>  PRT
<213>  Synthetic


<220>
<221>  PEPTIDE
<222>  (1)..(4)
<223>  Recognition site amino acid sequence cleavable by herpes simplex 
       virus protease

<220>
<221>  MISC_FEATURE
<222>  (1)..()
<223>  Xaa = valine or leucine or isoleucine (1st amino acid residue)

<220>
<221>  MISC_FEATURE
<222>  (2)..()
<223>  Xaa = serine or threonine or asparagine or glutamine or cysteine 
       or tyrosine (2nd amino acid residue)

<400>  13

Xaa Xaa Ala Ser 
1               


<210>  14
<211>  1000
<212>  DNA
<213>  human herpes simplex virus 2


<220>
<221>  CDS
<222>  (1)..(999)
<223>  HSV-2 gene encoding Herpes Simplex Virus 2 protease fused to 
       capsid protein (ICP35)

<400>  14
atg gcg tcg gcg gaa atg cgc gag cgg ttg gag gcg cct ctg ccc gac         48
Met Ala Ser Ala Glu Met Arg Glu Arg Leu Glu Ala Pro Leu Pro Asp           
1               5                   10                  15                

cgg gcg gtg ccc atc tac gtg gcc ggg ttt ttg gcc ctg tac gac agc         96
Arg Ala Val Pro Ile Tyr Val Ala Gly Phe Leu Ala Leu Tyr Asp Ser           
            20                  25                  30                    

ggg gac tcc ggc gag ctg gcc ctg gac cca gac acg gtg cgt gcg gcc        144
Gly Asp Ser Gly Glu Leu Ala Leu Asp Pro Asp Thr Val Arg Ala Ala           
        35                  40                  45                        

ctg cct ccg gag aac ccc ctg ccg atc aac gta gac cac cgc gct cgg        192
Leu Pro Pro Glu Asn Pro Leu Pro Ile Asn Val Asp His Arg Ala Arg           
    50                  55                  60                            

tgc gag gtg ggc cgg gtg ctc gcc gtg gtc aac gac cct cgg ggg ccg        240
Cys Glu Val Gly Arg Val Leu Ala Val Val Asn Asp Pro Arg Gly Pro           
65                  70                  75                  80            

ttt ttt gtg ggg ctg atc gcg tgc gtg cag ctg gag cgc gtc ctc gag        288
Phe Phe Val Gly Leu Ile Ala Cys Val Gln Leu Glu Arg Val Leu Glu           
                85                  90                  95                

acg gcc gcc agc gcc gct att ttt gag cgc cgc gga ccc gcg ctc tcc        336
Thr Ala Ala Ser Ala Ala Ile Phe Glu Arg Arg Gly Pro Ala Leu Ser           
            100                 105                 110                   

cgg gag gag cgt ctg ctg tac ctg atc acc aac tac ctg cca tcg gtc        384
Arg Glu Glu Arg Leu Leu Tyr Leu Ile Thr Asn Tyr Leu Pro Ser Val           
        115                 120                 125                       

tcg ctg tcc aca aaa cgc cgg ggg gac gag gtt ccg ccc gac ccc acc        432
Ser Leu Ser Thr Lys Arg Arg Gly Asp Glu Val Pro Pro Asp Pro Thr           
    130                 135                 140                           

ctg ttt gcg cac gtg gcc ctg tgc gcc atc ggg cgt cgc ctt gga acc        480
Leu Phe Ala His Val Ala Leu Cys Ala Ile Gly Arg Arg Leu Gly Thr           
145                 150                 155                 160           

atc gtc acc tac gac acc agc cta gac gcg gcc atc gct ccg ttt cgc        528
Ile Val Thr Tyr Asp Thr Ser Leu Asp Ala Ala Ile Ala Pro Phe Arg           
                165                 170                 175               

cac ctg gac ccg gcg acg cgc gag ggg gtg cga cgc gag gcc gcc gag        576
His Leu Asp Pro Ala Thr Arg Glu Gly Val Arg Arg Glu Ala Ala Glu           
            180                 185                 190                   

gcc gag ctc gcg ctg gcc ggg cgc acc tgg gcc ccc ggc gtg gag gcg        624
Ala Glu Leu Ala Leu Ala Gly Arg Thr Trp Ala Pro Gly Val Glu Ala           
        195                 200                 205                       

ctc aca cac acg ctg ctc tcc acc gcc gtc aac aac atg atg ctg cgt        672
Leu Thr His Thr Leu Leu Ser Thr Ala Val Asn Asn Met Met Leu Arg           
    210                 215                 220                           

gac cgc tgg agc ctc gtg gcc gag cgg cgg cgg cag gcc ggg atc gcc        720
Asp Arg Trp Ser Leu Val Ala Glu Arg Arg Arg Gln Ala Gly Ile Ala           
225                 230                 235                 240           

gga cac acg tac ctt cag gcg agc gaa aaa ttt aaa ata tgg ggg gcg        768
Gly His Thr Tyr Leu Gln Ala Ser Glu Lys Phe Lys Ile Trp Gly Ala           
                245                 250                 255               

gag tct gcc cct gcg ccg gag cgc ggg tat aaa acc ggc gcc ccg ggt        816
Glu Ser Ala Pro Ala Pro Glu Arg Gly Tyr Lys Thr Gly Ala Pro Gly           
            260                 265                 270                   

gcc atg gac aca tcc ccc gcc gcg agc gtt ccc gcg ccg cag gtc gcc        864
Ala Met Asp Thr Ser Pro Ala Ala Ser Val Pro Ala Pro Gln Val Ala           
        275                 280                 285                       

gtc cgt gcg cgt caa gtc gcg tcg tcg tcg tct tct tct ttt ccg gca        912
Val Arg Ala Arg Gln Val Ala Ser Ser Ser Ser Ser Ser Phe Pro Ala           
    290                 295                 300                           

ccg gcc gat atg aac ccc gtt tcg gca tcg ggc gcc ccg gcc cct ccg        960
Pro Ala Asp Met Asn Pro Val Ser Ala Ser Gly Ala Pro Ala Pro Pro           
305                 310                 315                 320           

ccg ccc ggc gac ggg agt tat ttg tgg atc ccc gcc tct c                 1000
Pro Pro Gly Asp Gly Ser Tyr Leu Trp Ile Pro Ala Ser                       
                325                 330                                   


<210>  15
<211>  333
<212>  PRT
<213>  human herpes simplex virus 2

<400>  15

Met Ala Ser Ala Glu Met Arg Glu Arg Leu Glu Ala Pro Leu Pro Asp 
1               5                   10                  15      


Arg Ala Val Pro Ile Tyr Val Ala Gly Phe Leu Ala Leu Tyr Asp Ser 
            20                  25                  30          


Gly Asp Ser Gly Glu Leu Ala Leu Asp Pro Asp Thr Val Arg Ala Ala 
        35                  40                  45              


Leu Pro Pro Glu Asn Pro Leu Pro Ile Asn Val Asp His Arg Ala Arg 
    50                  55                  60                  


Cys Glu Val Gly Arg Val Leu Ala Val Val Asn Asp Pro Arg Gly Pro 
65                  70                  75                  80  


Phe Phe Val Gly Leu Ile Ala Cys Val Gln Leu Glu Arg Val Leu Glu 
                85                  90                  95      


Thr Ala Ala Ser Ala Ala Ile Phe Glu Arg Arg Gly Pro Ala Leu Ser 
            100                 105                 110         


Arg Glu Glu Arg Leu Leu Tyr Leu Ile Thr Asn Tyr Leu Pro Ser Val 
        115                 120                 125             


Ser Leu Ser Thr Lys Arg Arg Gly Asp Glu Val Pro Pro Asp Pro Thr 
    130                 135                 140                 


Leu Phe Ala His Val Ala Leu Cys Ala Ile Gly Arg Arg Leu Gly Thr 
145                 150                 155                 160 


Ile Val Thr Tyr Asp Thr Ser Leu Asp Ala Ala Ile Ala Pro Phe Arg 
                165                 170                 175     


His Leu Asp Pro Ala Thr Arg Glu Gly Val Arg Arg Glu Ala Ala Glu 
            180                 185                 190         


Ala Glu Leu Ala Leu Ala Gly Arg Thr Trp Ala Pro Gly Val Glu Ala 
        195                 200                 205             


Leu Thr His Thr Leu Leu Ser Thr Ala Val Asn Asn Met Met Leu Arg 
    210                 215                 220                 


Asp Arg Trp Ser Leu Val Ala Glu Arg Arg Arg Gln Ala Gly Ile Ala 
225                 230                 235                 240 


Gly His Thr Tyr Leu Gln Ala Ser Glu Lys Phe Lys Ile Trp Gly Ala 
                245                 250                 255     


Glu Ser Ala Pro Ala Pro Glu Arg Gly Tyr Lys Thr Gly Ala Pro Gly 
            260                 265                 270         


Ala Met Asp Thr Ser Pro Ala Ala Ser Val Pro Ala Pro Gln Val Ala 
        275                 280                 285             


Val Arg Ala Arg Gln Val Ala Ser Ser Ser Ser Ser Ser Phe Pro Ala 
    290                 295                 300                 


Pro Ala Asp Met Asn Pro Val Ser Ala Ser Gly Ala Pro Ala Pro Pro 
305                 310                 315                 320 


Pro Pro Gly Asp Gly Ser Tyr Leu Trp Ile Pro Ala Ser 
                325                 330             


<210>  16
<211>  8
<212>  PRT
<213>  Synthetic


<220>
<221>  PEPTIDE
<222>  (1)..(8)
<223>  Recognition site amino acid sequence cleavable by herpes simplex 
       virus protease 2

<400>  16

Tyr Leu Gln Ala Ser Glu Leu Phe 
1               5               


<210>  17
<211>  913
<212>  DNA
<213>  Escherichia coli


<220>
<221>  rep_origin
<222>  (1)..(913)
<223>  p15A ori

<400>  17
gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg       60

cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga      120

tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg      180

aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg      240

aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca      300

cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc      360

gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt      420

cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg      480

cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct      540

tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag      600

cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa      660

actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag      720

ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag      780

caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa      840

tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata      900

cgatataagt tgt                                                         913


<210>  18
<211>  1011
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1011)
<223>  SpecR gene encoding spectomycin resistance protein

<400>  18
atg cgc tca cgc aac tgg tcc aga acc ttg acc gaa cgc agc ggt ggt         48
Met Arg Ser Arg Asn Trp Ser Arg Thr Leu Thr Glu Arg Ser Gly Gly           
1               5                   10                  15                

aac ggc gca gtg gcg gtt ttc atg gct tgt tat gac tgt ttt ttt ggg         96
Asn Gly Ala Val Ala Val Phe Met Ala Cys Tyr Asp Cys Phe Phe Gly           
            20                  25                  30                    

gta cag tct atg cct cgg gca tcc aag cag caa gcg cgt tac gcc gtg        144
Val Gln Ser Met Pro Arg Ala Ser Lys Gln Gln Ala Arg Tyr Ala Val           
        35                  40                  45                        

ggt cga tgt ttg atg tta tgg agc agc aac gat gtt acg cag cag ggc        192
Gly Arg Cys Leu Met Leu Trp Ser Ser Asn Asp Val Thr Gln Gln Gly           
    50                  55                  60                            

agt cgc cct aaa aca aag tta aac atc atg agg gaa gcg gtg atc gcc        240
Ser Arg Pro Lys Thr Lys Leu Asn Ile Met Arg Glu Ala Val Ile Ala           
65                  70                  75                  80            

gaa gta tcg act caa cta tca gag gta gtt ggc gtc atc gag cgc cat        288
Glu Val Ser Thr Gln Leu Ser Glu Val Val Gly Val Ile Glu Arg His           
                85                  90                  95                

ctc gaa ccg acg ttg ctg gcc gta cat ttg tac ggc tcc gca gtg gat        336
Leu Glu Pro Thr Leu Leu Ala Val His Leu Tyr Gly Ser Ala Val Asp           
            100                 105                 110                   

ggc ggc ctg aag cca cac agt gat att gat ttg ctg gtt acg gtg acc        384
Gly Gly Leu Lys Pro His Ser Asp Ile Asp Leu Leu Val Thr Val Thr           
        115                 120                 125                       

gta agg ctt gat gaa aca acg cgg cga gct ttg atc aac gac ctt ttg        432
Val Arg Leu Asp Glu Thr Thr Arg Arg Ala Leu Ile Asn Asp Leu Leu           
    130                 135                 140                           

gaa act tcg gct tcc cct gga gag agc gag att ctc cgc gct gta gaa        480
Glu Thr Ser Ala Ser Pro Gly Glu Ser Glu Ile Leu Arg Ala Val Glu           
145                 150                 155                 160           

gtc acc att gtt gtg cac gac gac atc att ccg tgg cgt tat cca gct        528
Val Thr Ile Val Val His Asp Asp Ile Ile Pro Trp Arg Tyr Pro Ala           
                165                 170                 175               

aag cgc gaa ctg caa ttt gga gaa tgg cag cgc aat gac att ctt gca        576
Lys Arg Glu Leu Gln Phe Gly Glu Trp Gln Arg Asn Asp Ile Leu Ala           
            180                 185                 190                   

ggt atc ttc gag cca gcc acg atc gac att gat ctg gct atc ttg ctg        624
Gly Ile Phe Glu Pro Ala Thr Ile Asp Ile Asp Leu Ala Ile Leu Leu           
        195                 200                 205                       

aca aaa gca aga gaa cat agc gtt gcc ttg gta ggt cca gcg gcg gag        672
Thr Lys Ala Arg Glu His Ser Val Ala Leu Val Gly Pro Ala Ala Glu           
    210                 215                 220                           

gaa ctc ttt gat ccg gtt cct gaa cag gat cta ttt gag gcg cta aat        720
Glu Leu Phe Asp Pro Val Pro Glu Gln Asp Leu Phe Glu Ala Leu Asn           
225                 230                 235                 240           

gaa acc tta acg cta tgg aac tcg ccg ccc gac tgg gct ggc gat gag        768
Glu Thr Leu Thr Leu Trp Asn Ser Pro Pro Asp Trp Ala Gly Asp Glu           
                245                 250                 255               

cga aat gta gtg ctt acg ttg tcc cgc att tgg tac agc gca gta acc        816
Arg Asn Val Val Leu Thr Leu Ser Arg Ile Trp Tyr Ser Ala Val Thr           
            260                 265                 270                   

ggc aaa atc gcg ccg aag gat gtc gct gcc gac tgg gca atg gag cgc        864
Gly Lys Ile Ala Pro Lys Asp Val Ala Ala Asp Trp Ala Met Glu Arg           
        275                 280                 285                       

ctg ccg gcc cag tat cag ccc gtc ata ctt gaa gct aga cag gct tat        912
Leu Pro Ala Gln Tyr Gln Pro Val Ile Leu Glu Ala Arg Gln Ala Tyr           
    290                 295                 300                           

ctt gga caa gaa gaa gat cgc ttg gcc tcg cgc gca gat cag ttg gaa        960
Leu Gly Gln Glu Glu Asp Arg Leu Ala Ser Arg Ala Asp Gln Leu Glu           
305                 310                 315                 320           

gaa ttt gtc cac tac gtg aaa ggc gag atc acc aag gta gtc ggc aaa       1008
Glu Phe Val His Tyr Val Lys Gly Glu Ile Thr Lys Val Val Gly Lys           
                325                 330                 335               

taa                                                                   1011


<210>  19
<211>  336
<212>  PRT
<213>  Escherichia coli

<400>  19

Met Arg Ser Arg Asn Trp Ser Arg Thr Leu Thr Glu Arg Ser Gly Gly 
1               5                   10                  15      


Asn Gly Ala Val Ala Val Phe Met Ala Cys Tyr Asp Cys Phe Phe Gly 
            20                  25                  30          


Val Gln Ser Met Pro Arg Ala Ser Lys Gln Gln Ala Arg Tyr Ala Val 
        35                  40                  45              


Gly Arg Cys Leu Met Leu Trp Ser Ser Asn Asp Val Thr Gln Gln Gly 
    50                  55                  60                  


Ser Arg Pro Lys Thr Lys Leu Asn Ile Met Arg Glu Ala Val Ile Ala 
65                  70                  75                  80  


Glu Val Ser Thr Gln Leu Ser Glu Val Val Gly Val Ile Glu Arg His 
                85                  90                  95      


Leu Glu Pro Thr Leu Leu Ala Val His Leu Tyr Gly Ser Ala Val Asp 
            100                 105                 110         


Gly Gly Leu Lys Pro His Ser Asp Ile Asp Leu Leu Val Thr Val Thr 
        115                 120                 125             


Val Arg Leu Asp Glu Thr Thr Arg Arg Ala Leu Ile Asn Asp Leu Leu 
    130                 135                 140                 


Glu Thr Ser Ala Ser Pro Gly Glu Ser Glu Ile Leu Arg Ala Val Glu 
145                 150                 155                 160 


Val Thr Ile Val Val His Asp Asp Ile Ile Pro Trp Arg Tyr Pro Ala 
                165                 170                 175     


Lys Arg Glu Leu Gln Phe Gly Glu Trp Gln Arg Asn Asp Ile Leu Ala 
            180                 185                 190         


Gly Ile Phe Glu Pro Ala Thr Ile Asp Ile Asp Leu Ala Ile Leu Leu 
        195                 200                 205             


Thr Lys Ala Arg Glu His Ser Val Ala Leu Val Gly Pro Ala Ala Glu 
    210                 215                 220                 


Glu Leu Phe Asp Pro Val Pro Glu Gln Asp Leu Phe Glu Ala Leu Asn 
225                 230                 235                 240 


Glu Thr Leu Thr Leu Trp Asn Ser Pro Pro Asp Trp Ala Gly Asp Glu 
                245                 250                 255     


Arg Asn Val Val Leu Thr Leu Ser Arg Ile Trp Tyr Ser Ala Val Thr 
            260                 265                 270         


Gly Lys Ile Ala Pro Lys Asp Val Ala Ala Asp Trp Ala Met Glu Arg 
        275                 280                 285             


Leu Pro Ala Gln Tyr Gln Pro Val Ile Leu Glu Ala Arg Gln Ala Tyr 
    290                 295                 300                 


Leu Gly Gln Glu Glu Asp Arg Leu Ala Ser Arg Ala Asp Gln Leu Glu 
305                 310                 315                 320 


Glu Phe Val His Tyr Val Lys Gly Glu Ile Thr Lys Val Val Gly Lys 
                325                 330                 335     


<210>  20
<211>  30
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(30)
<223>  Primer for insertion of HRP cleavage site into TetR protein

<400>  20
tcgagaagtt ctgtttcagg gtccggttta                                        30


<210>  21
<211>  30
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(30)
<223>  Primer for insertion of HRP cleavage site into TetR protein

<400>  21
tcgataaacc ggaccctgaa acagaacttc                                        30


<210>  22
<211>  24
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(24)
<223>  Primer for  of HRV 3Cpro gene amplification

<400>  22
gaattcatta aagaggagaa aggt                                              24


<210>  23
<211>  25
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(25)
<223>  Primer for  of HRV 3Cpro gene amplification

<400>  23
gtccacattg attatttgca cggcg                                             25


<210>  24
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 0 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues  55-56

<400>  24
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gag gtt tta ttc cag ggc cct gtg tac        192
Val Lys Thr Asp Leu Ser Gly Glu Val Leu Phe Gln Gly Pro Val Tyr           
    50                  55                  60                            

gcc ctg aat gaa ctg cag gac gag gca gcg cgg cta tcg tgg ctg gcc        240
Ala Leu Asn Glu Leu Gln Asp Glu Ala Ala Arg Leu Ser Trp Leu Ala           
65                  70                  75                  80            

acg acg ggc gtt cct tgc gca gct gtg ctc gac gtt gtc act gaa gcg        288
Thr Thr Gly Val Pro Cys Ala Ala Val Leu Asp Val Val Thr Glu Ala           
                85                  90                  95                

gga agg gac tgg ctg cta ttg ggc gaa gtg ccg ggg cag gat ctc ctg        336
Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu           
            100                 105                 110                   

tca tct cac ctt gct cct gcc gag aaa gta tcc atc atg gct gat gca        384
Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala           
        115                 120                 125                       

atg cgg cgg ctg cat acg ctt gat ccg gct acc tgc cca ttc gac cac        432
Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His           
    130                 135                 140                           

caa gcg aaa cat cgc atc gag cga gca cgt act cgg atg gaa gcc ggt        480
Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly           
145                 150                 155                 160           

ctt gtc gat cag gat gat ctg gac gaa gag cat cag ggg ctc gcg cca        528
Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro           
                165                 170                 175               

gcc gaa ctg ttc gcc agg ctc aag gcg cgc atg ccc gac ggc gag gat        576
Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp           
            180                 185                 190                   

ctc gtc gtg acc cat ggc gat gcc tgc ttg ccg aat atc atg gtg gaa        624
Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu           
        195                 200                 205                       

aat ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg        672
Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala           
    210                 215                 220                           

gac cgc tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag        720
Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu           
225                 230                 235                 240           

ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  25
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  25

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Glu Val Leu Phe Gln Gly Pro Val Tyr 
    50                  55                  60                  


Ala Leu Asn Glu Leu Gln Asp Glu Ala Ala Arg Leu Ser Trp Leu Ala 
65                  70                  75                  80  


Thr Thr Gly Val Pro Cys Ala Ala Val Leu Asp Val Val Thr Glu Ala 
                85                  90                  95      


Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu 
            100                 105                 110         


Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala 
        115                 120                 125             


Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His 
    130                 135                 140                 


Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly 
145                 150                 155                 160 


Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro 
                165                 170                 175     


Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp 
            180                 185                 190         


Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu 
        195                 200                 205             


Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala 
    210                 215                 220                 


Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu 
225                 230                 235                 240 


Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  26
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 1 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues 59-60-.

<400>  26
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa gag gtt tta ttc cag        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Glu Val Leu Phe Gln           
    50                  55                  60                            

ggc cct gtg tac ctg cag gac gag gca gcg cgg cta tcg tgg ctg gcc        240
Gly Pro Val Tyr Leu Gln Asp Glu Ala Ala Arg Leu Ser Trp Leu Ala           
65                  70                  75                  80            

acg acg ggc gtt cct tgc gca gct gtg ctc gac gtt gtc act gaa gcg        288
Thr Thr Gly Val Pro Cys Ala Ala Val Leu Asp Val Val Thr Glu Ala           
                85                  90                  95                

gga agg gac tgg ctg cta ttg ggc gaa gtg ccg ggg cag gat ctc ctg        336
Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu           
            100                 105                 110                   

tca tct cac ctt gct cct gcc gag aaa gta tcc atc atg gct gat gca        384
Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala           
        115                 120                 125                       

atg cgg cgg ctg cat acg ctt gat ccg gct acc tgc cca ttc gac cac        432
Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His           
    130                 135                 140                           

caa gcg aaa cat cgc atc gag cga gca cgt act cgg atg gaa gcc ggt        480
Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly           
145                 150                 155                 160           

ctt gtc gat cag gat gat ctg gac gaa gag cat cag ggg ctc gcg cca        528
Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro           
                165                 170                 175               

gcc gaa ctg ttc gcc agg ctc aag gcg cgc atg ccc gac ggc gag gat        576
Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp           
            180                 185                 190                   

ctc gtc gtg acc cat ggc gat gcc tgc ttg ccg aat atc atg gtg gaa        624
Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu           
        195                 200                 205                       

aat ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg        672
Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala           
    210                 215                 220                           

gac cgc tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag        720
Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu           
225                 230                 235                 240           

ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  27
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  27

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Glu Val Leu Phe Gln 
    50                  55                  60                  


Gly Pro Val Tyr Leu Gln Asp Glu Ala Ala Arg Leu Ser Trp Leu Ala 
65                  70                  75                  80  


Thr Thr Gly Val Pro Cys Ala Ala Val Leu Asp Val Val Thr Glu Ala 
                85                  90                  95      


Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu 
            100                 105                 110         


Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala 
        115                 120                 125             


Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His 
    130                 135                 140                 


Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly 
145                 150                 155                 160 


Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro 
                165                 170                 175     


Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp 
            180                 185                 190         


Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu 
        195                 200                 205             


Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala 
    210                 215                 220                 


Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu 
225                 230                 235                 240 


Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  28
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 2 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues 74-75.

<400>  28
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa ctg cag gac gag gca        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala           
    50                  55                  60                            

gcg cgg cta tcg tgg ctg gcc acg acg ggc gag gtt tta ttc cag ggc        240
Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Glu Val Leu Phe Gln Gly           
65                  70                  75                  80            

cct gtg tac gtt cct tgc gca gct gtg ctc gac gtt gtc act gaa gcg        288
Pro Val Tyr Val Pro Cys Ala Ala Val Leu Asp Val Val Thr Glu Ala           
                85                  90                  95                

gga agg gac tgg ctg cta ttg ggc gaa gtg ccg ggg cag gat ctc ctg        336
Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu           
            100                 105                 110                   

tca tct cac ctt gct cct gcc gag aaa gta tcc atc atg gct gat gca        384
Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala           
        115                 120                 125                       

atg cgg cgg ctg cat acg ctt gat ccg gct acc tgc cca ttc gac cac        432
Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His           
    130                 135                 140                           

caa gcg aaa cat cgc atc gag cga gca cgt act cgg atg gaa gcc ggt        480
Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly           
145                 150                 155                 160           

ctt gtc gat cag gat gat ctg gac gaa gag cat cag ggg ctc gcg cca        528
Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro           
                165                 170                 175               

gcc gaa ctg ttc gcc agg ctc aag gcg cgc atg ccc gac ggc gag gat        576
Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp           
            180                 185                 190                   

ctc gtc gtg acc cat ggc gat gcc tgc ttg ccg aat atc atg gtg gaa        624
Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu           
        195                 200                 205                       

aat ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg        672
Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala           
    210                 215                 220                           

gac cgc tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag        720
Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu           
225                 230                 235                 240           

ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  29
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  29

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Glu Val Leu Phe Gln Gly 
65                  70                  75                  80  


Pro Val Tyr Val Pro Cys Ala Ala Val Leu Asp Val Val Thr Glu Ala 
                85                  90                  95      


Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu 
            100                 105                 110         


Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala 
        115                 120                 125             


Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His 
    130                 135                 140                 


Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly 
145                 150                 155                 160 


Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro 
                165                 170                 175     


Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp 
            180                 185                 190         


Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu 
        195                 200                 205             


Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala 
    210                 215                 220                 


Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu 
225                 230                 235                 240 


Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  30
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 3 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues 87-88.

<400>  30
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa ctg cag gac gag gca        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala           
    50                  55                  60                            

gcg cgg cta tcg tgg ctg gcc acg acg ggc gtt cct tgc gca gct gtg        240
Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val           
65                  70                  75                  80            

ctc gac gtt gtc act gaa gcg gag gtt tta ttc cag ggc cct gtg tac        288
Leu Asp Val Val Thr Glu Ala Glu Val Leu Phe Gln Gly Pro Val Tyr           
                85                  90                  95                

gga agg gac tgg ctg cta ttg ggc gaa gtg ccg ggg cag gat ctc ctg        336
Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu           
            100                 105                 110                   

tca tct cac ctt gct cct gcc gag aaa gta tcc atc atg gct gat gca        384
Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala           
        115                 120                 125                       

atg cgg cgg ctg cat acg ctt gat ccg gct acc tgc cca ttc gac cac        432
Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His           
    130                 135                 140                           

caa gcg aaa cat cgc atc gag cga gca cgt act cgg atg gaa gcc ggt        480
Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly           
145                 150                 155                 160           

ctt gtc gat cag gat gat ctg gac gaa gag cat cag ggg ctc gcg cca        528
Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro           
                165                 170                 175               

gcc gaa ctg ttc gcc agg ctc aag gcg cgc atg ccc gac ggc gag gat        576
Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp           
            180                 185                 190                   

ctc gtc gtg acc cat ggc gat gcc tgc ttg ccg aat atc atg gtg gaa        624
Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu           
        195                 200                 205                       

aat ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg        672
Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala           
    210                 215                 220                           

gac cgc tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag        720
Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu           
225                 230                 235                 240           

ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  31
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  31

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Glu Val Leu Phe Gln Gly Pro Val Tyr 
                85                  90                  95      


Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu 
            100                 105                 110         


Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala 
        115                 120                 125             


Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His 
    130                 135                 140                 


Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly 
145                 150                 155                 160 


Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro 
                165                 170                 175     


Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp 
            180                 185                 190         


Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu 
        195                 200                 205             


Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala 
    210                 215                 220                 


Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu 
225                 230                 235                 240 


Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  32
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 4 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues 107-108.

<400>  32
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa ctg cag gac gag gca        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala           
    50                  55                  60                            

gcg cgg cta tcg tgg ctg gcc acg acg ggc gtt cct tgc gca gct gtg        240
Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val           
65                  70                  75                  80            

ctc gac gtt gtc act gaa gcg gga agg gac tgg ctg cta ttg ggc gaa        288
Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu           
                85                  90                  95                

gtg ccg ggg cag gat ctc ctg tca tct cac ctt gag gtt tta ttc cag        336
Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Glu Val Leu Phe Gln           
            100                 105                 110                   

ggc cct gtg tac gct cct gcc gag aaa gta tcc atc atg gct gat gca        384
Gly Pro Val Tyr Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala           
        115                 120                 125                       

atg cgg cgg ctg cat acg ctt gat ccg gct acc tgc cca ttc gac cac        432
Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His           
    130                 135                 140                           

caa gcg aaa cat cgc atc gag cga gca cgt act cgg atg gaa gcc ggt        480
Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly           
145                 150                 155                 160           

ctt gtc gat cag gat gat ctg gac gaa gag cat cag ggg ctc gcg cca        528
Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro           
                165                 170                 175               

gcc gaa ctg ttc gcc agg ctc aag gcg cgc atg ccc gac ggc gag gat        576
Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp           
            180                 185                 190                   

ctc gtc gtg acc cat ggc gat gcc tgc ttg ccg aat atc atg gtg gaa        624
Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu           
        195                 200                 205                       

aat ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg        672
Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala           
    210                 215                 220                           

gac cgc tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag        720
Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu           
225                 230                 235                 240           

ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  33
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  33

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu 
                85                  90                  95      


Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Glu Val Leu Phe Gln 
            100                 105                 110         


Gly Pro Val Tyr Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala 
        115                 120                 125             


Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His 
    130                 135                 140                 


Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly 
145                 150                 155                 160 


Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro 
                165                 170                 175     


Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp 
            180                 185                 190         


Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu 
        195                 200                 205             


Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala 
    210                 215                 220                 


Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu 
225                 230                 235                 240 


Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  34
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 5 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues 129-130.

<400>  34
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa ctg cag gac gag gca        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala           
    50                  55                  60                            

gcg cgg cta tcg tgg ctg gcc acg acg ggc gtt cct tgc gca gct gtg        240
Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val           
65                  70                  75                  80            

ctc gac gtt gtc act gaa gcg gga agg gac tgg ctg cta ttg ggc gaa        288
Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu           
                85                  90                  95                

gtg ccg ggg cag gat ctc ctg tca tct cac ctt gct cct gcc gag aaa        336
Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys           
            100                 105                 110                   

gta tcc atc atg gct gat gca atg cgg cgg ctg cat acg ctt gat ccg        384
Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro           
        115                 120                 125                       

gct gag gtt tta ttc cag ggc cct gtg tac acc tgc cca ttc gac cac        432
Ala Glu Val Leu Phe Gln Gly Pro Val Tyr Thr Cys Pro Phe Asp His           
    130                 135                 140                           

caa gcg aaa cat cgc atc gag cga gca cgt act cgg atg gaa gcc ggt        480
Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly           
145                 150                 155                 160           

ctt gtc gat cag gat gat ctg gac gaa gag cat cag ggg ctc gcg cca        528
Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro           
                165                 170                 175               

gcc gaa ctg ttc gcc agg ctc aag gcg cgc atg ccc gac ggc gag gat        576
Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp           
            180                 185                 190                   

ctc gtc gtg acc cat ggc gat gcc tgc ttg ccg aat atc atg gtg gaa        624
Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu           
        195                 200                 205                       

aat ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg        672
Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala           
    210                 215                 220                           

gac cgc tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag        720
Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu           
225                 230                 235                 240           

ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  35
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  35

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu 
                85                  90                  95      


Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys 
            100                 105                 110         


Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro 
        115                 120                 125             


Ala Glu Val Leu Phe Gln Gly Pro Val Tyr Thr Cys Pro Phe Asp His 
    130                 135                 140                 


Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly 
145                 150                 155                 160 


Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro 
                165                 170                 175     


Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp 
            180                 185                 190         


Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu 
        195                 200                 205             


Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala 
    210                 215                 220                 


Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu 
225                 230                 235                 240 


Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  36
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 6 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues 181-182

<400>  36
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa ctg cag gac gag gca        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala           
    50                  55                  60                            

gcg cgg cta tcg tgg ctg gcc acg acg ggc gtt cct tgc gca gct gtg        240
Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val           
65                  70                  75                  80            

ctc gac gtt gtc act gaa gcg gga agg gac tgg ctg cta ttg ggc gaa        288
Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu           
                85                  90                  95                

gtg ccg ggg cag gat ctc ctg tca tct cac ctt gct cct gcc gag aaa        336
Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys           
            100                 105                 110                   

gta tcc atc atg gct gat gca atg cgg cgg ctg cat acg ctt gat ccg        384
Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro           
        115                 120                 125                       

gct acc tgc cca ttc gac cac caa gcg aaa cat cgc atc gag cga gca        432
Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala           
    130                 135                 140                           

cgt act cgg atg gaa gcc ggt ctt gtc gat cag gat gat ctg gac gaa        480
Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu           
145                 150                 155                 160           

gag cat cag ggg ctc gcg cca gcc gaa ctg ttc gcc agg ctc aag gcg        528
Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala           
                165                 170                 175               

cgc atg ccc gac ggc gag gtt tta ttc cag ggc cct gtg tac gag gat        576
Arg Met Pro Asp Gly Glu Val Leu Phe Gln Gly Pro Val Tyr Glu Asp           
            180                 185                 190                   

ctc gtc gtg acc cat ggc gat gcc tgc ttg ccg aat atc atg gtg gaa        624
Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu           
        195                 200                 205                       

aat ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg        672
Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala           
    210                 215                 220                           

gac cgc tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag        720
Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu           
225                 230                 235                 240           

ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  37
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  37

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu 
                85                  90                  95      


Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys 
            100                 105                 110         


Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro 
        115                 120                 125             


Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala 
    130                 135                 140                 


Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu 
145                 150                 155                 160 


Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala 
                165                 170                 175     


Arg Met Pro Asp Gly Glu Val Leu Phe Gln Gly Pro Val Tyr Glu Asp 
            180                 185                 190         


Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu 
        195                 200                 205             


Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala 
    210                 215                 220                 


Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu 
225                 230                 235                 240 


Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  38
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 7 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues 200-201

<400>  38
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa ctg cag gac gag gca        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala           
    50                  55                  60                            

gcg cgg cta tcg tgg ctg gcc acg acg ggc gtt cct tgc gca gct gtg        240
Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val           
65                  70                  75                  80            

ctc gac gtt gtc act gaa gcg gga agg gac tgg ctg cta ttg ggc gaa        288
Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu           
                85                  90                  95                

gtg ccg ggg cag gat ctc ctg tca tct cac ctt gct cct gcc gag aaa        336
Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys           
            100                 105                 110                   

gta tcc atc atg gct gat gca atg cgg cgg ctg cat acg ctt gat ccg        384
Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro           
        115                 120                 125                       

gct acc tgc cca ttc gac cac caa gcg aaa cat cgc atc gag cga gca        432
Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala           
    130                 135                 140                           

cgt act cgg atg gaa gcc ggt ctt gtc gat cag gat gat ctg gac gaa        480
Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu           
145                 150                 155                 160           

gag cat cag ggg ctc gcg cca gcc gaa ctg ttc gcc agg ctc aag gcg        528
Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala           
                165                 170                 175               

cgc atg ccc gac ggc gag gat ctc gtc gtg acc cat ggc gat gcc tgc        576
Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys           
            180                 185                 190                   

ttg ccg aat atc atg gtg gaa aat gag gtt tta ttc cag ggc cct gtg        624
Leu Pro Asn Ile Met Val Glu Asn Glu Val Leu Phe Gln Gly Pro Val           
        195                 200                 205                       

tac ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg        672
Tyr Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala           
    210                 215                 220                           

gac cgc tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag        720
Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu           
225                 230                 235                 240           

ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  39
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  39

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu 
                85                  90                  95      


Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys 
            100                 105                 110         


Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro 
        115                 120                 125             


Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala 
    130                 135                 140                 


Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu 
145                 150                 155                 160 


Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala 
                165                 170                 175     


Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys 
            180                 185                 190         


Leu Pro Asn Ile Met Val Glu Asn Glu Val Leu Phe Gln Gly Pro Val 
        195                 200                 205             


Tyr Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala 
    210                 215                 220                 


Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu 
225                 230                 235                 240 


Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  40
<211>  822
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(822)
<223>  mKanR - 8 gene encoding kanamycin resistance protein comprising 
       rhinovirus protease cleavable recognition site amino acid 
       sequence inserted between amino acid residues 233-234

<400>  40
atg att gaa caa gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg         48
Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val           
1               5                   10                  15                

gag agg cta ttc ggc tat gac tgg gca caa cag aca atc ggc tgc tct         96
Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser           
            20                  25                  30                    

gat gcc gcc gtg ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt        144
Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe           
        35                  40                  45                        

gtc aag acc gac ctg tcc ggt gcc ctg aat gaa ctg cag gac gag gca        192
Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala           
    50                  55                  60                            

gcg cgg cta tcg tgg ctg gcc acg acg ggc gtt cct tgc gca gct gtg        240
Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val           
65                  70                  75                  80            

ctc gac gtt gtc act gaa gcg gga agg gac tgg ctg cta ttg ggc gaa        288
Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu           
                85                  90                  95                

gtg ccg ggg cag gat ctc ctg tca tct cac ctt gct cct gcc gag aaa        336
Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys           
            100                 105                 110                   

gta tcc atc atg gct gat gca atg cgg cgg ctg cat acg ctt gat ccg        384
Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro           
        115                 120                 125                       

gct acc tgc cca ttc gac cac caa gcg aaa cat cgc atc gag cga gca        432
Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala           
    130                 135                 140                           

cgt act cgg atg gaa gcc ggt ctt gtc gat cag gat gat ctg gac gaa        480
Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu           
145                 150                 155                 160           

gag cat cag ggg ctc gcg cca gcc gaa ctg ttc gcc agg ctc aag gcg        528
Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala           
                165                 170                 175               

cgc atg ccc gac ggc gag gat ctc gtc gtg acc cat ggc gat gcc tgc        576
Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys           
            180                 185                 190                   

ttg ccg aat atc atg gtg gaa aat ggc cgc ttt tct gga ttc atc gac        624
Leu Pro Asn Ile Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile Asp           
        195                 200                 205                       

tgt ggc cgg ctg ggt gtg gcg gac cgc tat cag gac ata gcg ttg gct        672
Cys Gly Arg Leu Gly Val Ala Asp Arg Tyr Gln Asp Ile Ala Leu Ala           
    210                 215                 220                           

acc cgt gat att gct gaa gag ctt ggc gag gtt tta ttc cag ggc cct        720
Thr Arg Asp Ile Ala Glu Glu Leu Gly Glu Val Leu Phe Gln Gly Pro           
225                 230                 235                 240           

gtg tac ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc        768
Val Tyr Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala           
                245                 250                 255               

gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc        816
Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe           
            260                 265                 270                   

ttc tga                                                                822
Phe                                                                       
                                                                          


<210>  41
<211>  273
<212>  PRT
<213>  Escherichia coli

<400>  41

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
    50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu 
                85                  90                  95      


Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys 
            100                 105                 110         


Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro 
        115                 120                 125             


Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala 
    130                 135                 140                 


Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu 
145                 150                 155                 160 


Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala 
                165                 170                 175     


Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys 
            180                 185                 190         


Leu Pro Asn Ile Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile Asp 
        195                 200                 205             


Cys Gly Arg Leu Gly Val Ala Asp Arg Tyr Gln Asp Ile Ala Leu Ala 
    210                 215                 220                 


Thr Arg Asp Ile Ala Glu Glu Leu Gly Glu Val Leu Phe Gln Gly Pro 
225                 230                 235                 240 


Val Tyr Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala 
                245                 250                 255     


Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe 
            260                 265                 270         


Phe 
    


<210>  42
<211>  45
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(45)
<223>  Primer EH76  for encoding insertion of HRP cleavage site into 
       KanR protein

<400>  42
agggcccugg aataaaacct caccggacag gtcggtcttg acaaa                       45


<210>  43
<211>  37
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(37)
<223>  Primer EH77  for encoding insertion of HRP cleavage site into 
       KanR protein

<400>  43
agggcccugt gtacgccctg aatgaactgc aggacga                                37


<210>  44
<211>  45
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(45)
<223>  Primer EH78  for encoding insertion of HRP cleavage site into 
       KanR protein

<400>  44
agggcccugg aataaaacct cttcattcag ggcaccggac aggtc                       45


<210>  45
<211>  32
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(32)
<223>  Primer EH79  for encoding insertion of HRP cleavage site into 
       KanR protein

<400>  45
agggcccugt gtacctgcag gacgaggcag cg                                     32


<210>  46
<211>  40
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  Primer EH80  for encoding insertion of HRP cleavage site into 
       KanR protein

<400>  46
agggcccugg aataaaacct cgcccgtcgt ggccagccac                             40


<210>  47
<211>  35
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(35)
<223>  Primer EH81 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  47
agggcccugt gtacgttcct tgcgcagctg tgctc                                  35


<210>  48
<211>  43
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(43)
<223>  Primer EH82 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  48
agggcccugg aataaaacct ccgcttcagt gacaacgtcg agc                         43


<210>  49
<211>  37
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(37)
<223>  Primer EH83 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  49
agggcccugt gtacggaagg gactggctgc tattggg                                37


<210>  50
<211>  45
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(45)
<223>  Primer EH84 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  50
agggcccugg aataaaacct caaggtgaga tgacaggaga tcctg                       45


<210>  51
<211>  38
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(38)
<223>  Primer EH85 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  51
agggcccugt gtacgctcct gccgagaaag tatccatc                               38


<210>  52
<211>  42
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(42)
<223>  Primer EH86 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  52
agggcccugg aataaaacct cagccggatc aagcgtatgc ag                          42


<210>  53
<211>  37
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(37)
<223>  Primer EH87 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  53
agggcccugt gtacacctgc ccattcgacc accaagc                                37


<210>  54
<211>  39
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(39)
<223>  Primer EH88 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  54
agggcccugg aataaaacct cgccgtcggg catgcgcgc                              39


<210>  55
<211>  38
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(38)
<223>  Primer EH89 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  55
agggcccugt gtacgaggat ctcgtcgtga cccatggc                               38


<210>  56
<211>  45
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(45)
<223>  Primer EH90 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  56
agggcccugg aataaaacct cattttccac catgatattc ggcaa                       45


<210>  57
<211>  35
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(35)
<223>  Primer EH91 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  57
agggcccugt gtacggccgc ttttctggat tcatc                                  35


<210>  58
<211>  43
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(43)
<223>  Primer EH92 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  58
agggcccugg aataaaacct cgccaagctc ttcagcaata tca                         43


<210>  59
<211>  32
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(32)
<223>  Primer EH93 for encoding insertion of HRP cleavage site into KanR
       protein

<400>  59
agggcccugt gtacggcgaa tgggctgacc gc                                     32


<210>  60
<211>  25
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(25)
<223>  Primer EH97 for amplifying each of the nine modified KanR

<400>  60
aagctcucga accccagagt cccgc                                             25


<210>  61
<211>  26
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(26)
<223>  Primer EH98 for amplifying each of the nine modified KanR

<400>  61
acagacgucg gaattgccag ctgggg                                            26


<210>  62
<211>  32
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(32)
<223>  Primer EH99 for cloning into pEH500

<400>  62
acgtctguca gaccaagttt actcgaactt cc                                     32


<210>  63
<211>  25
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(25)
<223>  Primer pEH100 for cloning into pEH500 plasmid

<400>  63
agagctuccg cgcacatttc cccga                                             25


<210>  64
<211>  30
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(30)
<223>  Primer EH101 for amplifying mKan55 gene

<400>  64
accccagagu cccgctcaga agaactcgtc                                        30


<210>  65
<211>  29
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  Primer EH102 for amplifying mKan55 gene

<400>  65
atggttcuta gacgtcggaa ttgccagct                                         29


<210>  66
<211>  32
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(32)
<223>  Primer EH103

<400>  66
agaaccaugg aaaaaatcct tagctttcgc ta                                     32


<210>  67
<211>  34
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(34)
<223>  Primer EH104

<400>  67
actctggggu tcgacaggtc gcagacgttt tgca                                   34


<210>  68
<211>  26
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(26)
<223>  Primer EH105

<400>  68
aggggcguat gggctgcagc cgcctt                                            26


<210>  69
<211>  32
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(32)
<223>  Primer EH106

<400>  69
aatgccaugt aatactgcag tcacactttt ac                                     32


<210>  70
<211>  27
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(27)
<223>  Primer EH107

<400>  70
atggcatugg cggccttgct gttcttc                                           27


<210>  71
<211>  29
<212>  DNA
<213>  Synthetic


<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  Primer EH108

<400>  71
acgccccuga cgatgcgtgg agaccgaaa                                         29


<210>  72
<211>  1294
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (77)..(1294)
<223>  mtetR gene encoding modified tetracycline resistance protein 
       comprising recognition site sequence cleavable by HRV protease

<400>  72
ttgacagctt atcatcgata agctttaatg cggtagttta tcacagttaa attgctaacg       60

cagtcaggca ccgtgt atg aaa tct aac aat gcg ctc atc gtc atc ctc ggc      112
                  Met Lys Ser Asn Asn Ala Leu Ile Val Ile Leu Gly         
                  1               5                   10                  

acc gtc acc ctg gat gct gta ggc ata ggc ttg gtt atg ccg gta ctg        160
Thr Val Thr Leu Asp Ala Val Gly Ile Gly Leu Val Met Pro Val Leu           
        15                  20                  25                        

ccg ggc ctc ttg cgg gat atc gtc cat tcc gac agc atc gcc agt cac        208
Pro Gly Leu Leu Arg Asp Ile Val His Ser Asp Ser Ile Ala Ser His           
    30                  35                  40                            

tat ggc gtg ctg cta gcg cta tat gcg ttg atg caa ttt cta tgc gca        256
Tyr Gly Val Leu Leu Ala Leu Tyr Ala Leu Met Gln Phe Leu Cys Ala           
45                  50                  55                  60            

ccc gtt ctc gga gca ctg tcc gac cgc ttt ggc cgc cgc cca gtc ctg        304
Pro Val Leu Gly Ala Leu Ser Asp Arg Phe Gly Arg Arg Pro Val Leu           
                65                  70                  75                

ctc gct tcg cta ctt gga gcc act atc gac tac gcg atc atg gcg acc        352
Leu Ala Ser Leu Leu Gly Ala Thr Ile Asp Tyr Ala Ile Met Ala Thr           
            80                  85                  90                    

aca ccc gtc ctg tgg atc ctc tac gcc gga cgc atc gtg gcc ggc atc        400
Thr Pro Val Leu Trp Ile Leu Tyr Ala Gly Arg Ile Val Ala Gly Ile           
        95                  100                 105                       

acc ggc gcc aca ggt gcg gtt gct ggc gcc tat atc gcc gac atc acc        448
Thr Gly Ala Thr Gly Ala Val Ala Gly Ala Tyr Ile Ala Asp Ile Thr           
    110                 115                 120                           

gat ggg gaa gat cgg gct cgc cac ttc ggg ctc atg agc gct tgt ttc        496
Asp Gly Glu Asp Arg Ala Arg His Phe Gly Leu Met Ser Ala Cys Phe           
125                 130                 135                 140           

ggc gtg ggt atg gtg gca ggc ccc gtg gcc ggg gga ctg ttg ggc gcc        544
Gly Val Gly Met Val Ala Gly Pro Val Ala Gly Gly Leu Leu Gly Ala           
                145                 150                 155               

atc tcc ttg cat gca cca ttc ctt gcg gcg gcg gtg ctc aac ggc ctc        592
Ile Ser Leu His Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu           
            160                 165                 170                   

aac cta cta ctg ggc tgc ttc cta atg cag gag tcg cat aag gga gag        640
Asn Leu Leu Leu Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu           
        175                 180                 185                       

cgt gaa gtt ctg ttt cag ggt ccg gtt tat cga acg atg ccc ttg aga        688
Arg Glu Val Leu Phe Gln Gly Pro Val Tyr Arg Thr Met Pro Leu Arg           
    190                 195                 200                           

gcc ttc aac cca gtc agc tcc ttc cgg tgg gcg cgg ggc atg act atc        736
Ala Phe Asn Pro Val Ser Ser Phe Arg Trp Ala Arg Gly Met Thr Ile           
205                 210                 215                 220           

gtc gcc gca ctt atg act gtc ttc ttt atc atg caa ctc gta gga cag        784
Val Ala Ala Leu Met Thr Val Phe Phe Ile Met Gln Leu Val Gly Gln           
                225                 230                 235               

gtg ccg gca gcg ctc tgg gtc att ttc ggc gag gac cgc ttt cgc tgg        832
Val Pro Ala Ala Leu Trp Val Ile Phe Gly Glu Asp Arg Phe Arg Trp           
            240                 245                 250                   

agc gcg acg atg atc ggc ctg tcg ctt gcg gta ttc gga atc ttg cac        880
Ser Ala Thr Met Ile Gly Leu Ser Leu Ala Val Phe Gly Ile Leu His           
        255                 260                 265                       

gcc ctc gct caa gcc ttc gtc act ggt ccc gcc acc aaa cgt ttc ggc        928
Ala Leu Ala Gln Ala Phe Val Thr Gly Pro Ala Thr Lys Arg Phe Gly           
    270                 275                 280                           

gag aag cag gcc att atc gcc ggc atg gcg gcc gac gcg ctg ggc tac        976
Glu Lys Gln Ala Ile Ile Ala Gly Met Ala Ala Asp Ala Leu Gly Tyr           
285                 290                 295                 300           

gtc ttg ctg gcg ttc gcg acg cga ggc tgg atg gcc ttc ccc att atg       1024
Val Leu Leu Ala Phe Ala Thr Arg Gly Trp Met Ala Phe Pro Ile Met           
                305                 310                 315               

att ctt ctc gct tcc ggc ggc atc ggg atg ccc gcg ttg cag gcc atg       1072
Ile Leu Leu Ala Ser Gly Gly Ile Gly Met Pro Ala Leu Gln Ala Met           
            320                 325                 330                   

ctg tcc agg cag gta gat gac gac cat cag gga cag ctt caa gga tcg       1120
Leu Ser Arg Gln Val Asp Asp Asp His Gln Gly Gln Leu Gln Gly Ser           
        335                 340                 345                       

ctc gcg gct ctt acc agc cta act tcg atc act gga ccg ctg atc gtc       1168
Leu Ala Ala Leu Thr Ser Leu Thr Ser Ile Thr Gly Pro Leu Ile Val           
    350                 355                 360                           

acg gcg att tat gcc gcc tcg gcg agc aca tgg aac ggg ttg gca tgg       1216
Thr Ala Ile Tyr Ala Ala Ser Ala Ser Thr Trp Asn Gly Leu Ala Trp           
365                 370                 375                 380           

att gta ggc gcc gcc cta tac ctt gtc tgc ctc ccc gcg ttg cgt cgc       1264
Ile Val Gly Ala Ala Leu Tyr Leu Val Cys Leu Pro Ala Leu Arg Arg           
                385                 390                 395               

ggt gca tgg agc cgg gcc acc tcg acc tga                               1294
Gly Ala Trp Ser Arg Ala Thr Ser Thr                                       
            400                 405                                       


<210>  73
<211>  405
<212>  PRT
<213>  Escherichia coli

<400>  73

Met Lys Ser Asn Asn Ala Leu Ile Val Ile Leu Gly Thr Val Thr Leu 
1               5                   10                  15      


Asp Ala Val Gly Ile Gly Leu Val Met Pro Val Leu Pro Gly Leu Leu 
            20                  25                  30          


Arg Asp Ile Val His Ser Asp Ser Ile Ala Ser His Tyr Gly Val Leu 
        35                  40                  45              


Leu Ala Leu Tyr Ala Leu Met Gln Phe Leu Cys Ala Pro Val Leu Gly 
    50                  55                  60                  


Ala Leu Ser Asp Arg Phe Gly Arg Arg Pro Val Leu Leu Ala Ser Leu 
65                  70                  75                  80  


Leu Gly Ala Thr Ile Asp Tyr Ala Ile Met Ala Thr Thr Pro Val Leu 
                85                  90                  95      


Trp Ile Leu Tyr Ala Gly Arg Ile Val Ala Gly Ile Thr Gly Ala Thr 
            100                 105                 110         


Gly Ala Val Ala Gly Ala Tyr Ile Ala Asp Ile Thr Asp Gly Glu Asp 
        115                 120                 125             


Arg Ala Arg His Phe Gly Leu Met Ser Ala Cys Phe Gly Val Gly Met 
    130                 135                 140                 


Val Ala Gly Pro Val Ala Gly Gly Leu Leu Gly Ala Ile Ser Leu His 
145                 150                 155                 160 


Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu Asn Leu Leu Leu 
                165                 170                 175     


Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu Arg Glu Val Leu 
            180                 185                 190         


Phe Gln Gly Pro Val Tyr Arg Thr Met Pro Leu Arg Ala Phe Asn Pro 
        195                 200                 205             


Val Ser Ser Phe Arg Trp Ala Arg Gly Met Thr Ile Val Ala Ala Leu 
    210                 215                 220                 


Met Thr Val Phe Phe Ile Met Gln Leu Val Gly Gln Val Pro Ala Ala 
225                 230                 235                 240 


Leu Trp Val Ile Phe Gly Glu Asp Arg Phe Arg Trp Ser Ala Thr Met 
                245                 250                 255     


Ile Gly Leu Ser Leu Ala Val Phe Gly Ile Leu His Ala Leu Ala Gln 
            260                 265                 270         


Ala Phe Val Thr Gly Pro Ala Thr Lys Arg Phe Gly Glu Lys Gln Ala 
        275                 280                 285             


Ile Ile Ala Gly Met Ala Ala Asp Ala Leu Gly Tyr Val Leu Leu Ala 
    290                 295                 300                 


Phe Ala Thr Arg Gly Trp Met Ala Phe Pro Ile Met Ile Leu Leu Ala 
305                 310                 315                 320 


Ser Gly Gly Ile Gly Met Pro Ala Leu Gln Ala Met Leu Ser Arg Gln 
                325                 330                 335     


Val Asp Asp Asp His Gln Gly Gln Leu Gln Gly Ser Leu Ala Ala Leu 
            340                 345                 350         


Thr Ser Leu Thr Ser Ile Thr Gly Pro Leu Ile Val Thr Ala Ile Tyr 
        355                 360                 365             


Ala Ala Ser Ala Ser Thr Trp Asn Gly Leu Ala Trp Ile Val Gly Ala 
    370                 375                 380                 


Ala Leu Tyr Leu Val Cys Leu Pro Ala Leu Arg Arg Gly Ala Trp Ser 
385                 390                 395                 400 


Arg Ala Thr Ser Thr 
                405 


<210>  74
<211>  652
<212>  DNA
<213>  Human rhinovirus


<220>
<221>  gene
<222>  (1)..(652)
<223>  gbEH2 reverse complement sequence

<400>  74
ctgggtatcg gtaaaatagc tacgcagcag cattgcgcta aagccatcac gaccattacc       60

accaacatga atgcccagaa tctgaccgat tttatacaga acaccaccac aataaccgct      120

tttggtcgga taattgtatt tcagcatacg tgcggtctga ttaccgctca gcagaatatt      180

gccatagctc acaacatcac caactttgat aatggtcggt tcaacctgat ttgcgctcag      240

tgccagatta cattccggat aatcatcttc ggtttccggg atatatttgc ggatatcgcg      300

aaatttttca ttgcgatcca gctgaataac ggtgatttcc agtttcacac catcacgatt      360

atacagatca tagctatcca gcactttggt atgaatacca ttaacctgaa cttcacgacc      420

cggatctgca tgggtcggaa taatcagggt acgatcataa atacccagac cggtaaattt      480

gccgttatcg gtggtaataa cgcaggtatt gtttttcaga atgctacgac caaattcttc      540

ttccggaccc atctagtatt tctcctcttt ctctgctagc ataataccta ggactgagct      600

agctgtaaaa gtgtgactgc agtattacat ggcattggcg gccttgctgt tc              652


<210>  75
<211>  1291
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (77)..(1291)
<223>  mTetR gene encoding tetracyline resistance protein comprising 
       recognition site sequence cleabable by HIV protease

<400>  75
ttgacagctt atcatcgata agctttaatg cggtagttta tcacagttaa attgctaacg       60

cagtcaggca ccgtgt atg aaa tct aac aat gcg ctc atc gtc atc ctc ggc      112
                  Met Lys Ser Asn Asn Ala Leu Ile Val Ile Leu Gly         
                  1               5                   10                  

acc gtc acc ctg gat gct gta ggc ata ggc ttg gtt atg ccg gta ctg        160
Thr Val Thr Leu Asp Ala Val Gly Ile Gly Leu Val Met Pro Val Leu           
        15                  20                  25                        

ccg ggc ctc ttg cgg gat atc gtc cat tcc gac agc atc gcc agt cac        208
Pro Gly Leu Leu Arg Asp Ile Val His Ser Asp Ser Ile Ala Ser His           
    30                  35                  40                            

tat ggc gtg ctg cta gcg cta tat gcg ttg atg caa ttt cta tgc gca        256
Tyr Gly Val Leu Leu Ala Leu Tyr Ala Leu Met Gln Phe Leu Cys Ala           
45                  50                  55                  60            

ccc gtt ctc gga gca ctg tcc gac cgc ttt ggc cgc cgc cca gtc ctg        304
Pro Val Leu Gly Ala Leu Ser Asp Arg Phe Gly Arg Arg Pro Val Leu           
                65                  70                  75                

ctc gct tcg cta ctt gga gcc act atc gac tac gcg atc atg gcg acc        352
Leu Ala Ser Leu Leu Gly Ala Thr Ile Asp Tyr Ala Ile Met Ala Thr           
            80                  85                  90                    

aca ccc gtc ctg tgg atc ctc tac gcc gga cgc atc gtg gcc ggc atc        400
Thr Pro Val Leu Trp Ile Leu Tyr Ala Gly Arg Ile Val Ala Gly Ile           
        95                  100                 105                       

acc ggc gcc aca ggt gcg gtt gct ggc gcc tat atc gcc gac atc acc        448
Thr Gly Ala Thr Gly Ala Val Ala Gly Ala Tyr Ile Ala Asp Ile Thr           
    110                 115                 120                           

gat ggg gaa gat cgg gct cgc cac ttc ggg ctc atg agc gct tgt ttc        496
Asp Gly Glu Asp Arg Ala Arg His Phe Gly Leu Met Ser Ala Cys Phe           
125                 130                 135                 140           

ggc gtg ggt atg gtg gca ggc ccc gtg gcc ggg gga ctg ttg ggc gcc        544
Gly Val Gly Met Val Ala Gly Pro Val Ala Gly Gly Leu Leu Gly Ala           
                145                 150                 155               

atc tcc ttg cat gca cca ttc ctt gcg gcg gcg gtg ctc aac ggc ctc        592
Ile Ser Leu His Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu           
            160                 165                 170                   

aac cta cta ctg ggc tgc ttc cta atg cag gag tcg cat aag gga gag        640
Asn Leu Leu Leu Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu           
        175                 180                 185                       

cgt cga agt cag aac tac ccg atc gtt cga ccg atg ccc ttg aga gcc        688
Arg Arg Ser Gln Asn Tyr Pro Ile Val Arg Pro Met Pro Leu Arg Ala           
    190                 195                 200                           

ttc aac cca gtc agc tcc ttc cgg tgg gcg cgg ggc atg act atc gtc        736
Phe Asn Pro Val Ser Ser Phe Arg Trp Ala Arg Gly Met Thr Ile Val           
205                 210                 215                 220           

gcc gca ctt atg act gtc ttc ttt atc atg caa ctc gta gga cag gtg        784
Ala Ala Leu Met Thr Val Phe Phe Ile Met Gln Leu Val Gly Gln Val           
                225                 230                 235               

ccg gca gcg ctc tgg gtc att ttc ggc gag gac cgc ttt cgc tgg agc        832
Pro Ala Ala Leu Trp Val Ile Phe Gly Glu Asp Arg Phe Arg Trp Ser           
            240                 245                 250                   

gcg acg atg atc ggc ctg tcg ctt gcg gta ttc gga atc ttg cac gcc        880
Ala Thr Met Ile Gly Leu Ser Leu Ala Val Phe Gly Ile Leu His Ala           
        255                 260                 265                       

ctc gct caa gcc ttc gtc act ggt ccc gcc acc aaa cgt ttc ggc gag        928
Leu Ala Gln Ala Phe Val Thr Gly Pro Ala Thr Lys Arg Phe Gly Glu           
    270                 275                 280                           

aag cag gcc att atc gcc ggc atg gcg gcc gac gcg ctg ggc tac gtc        976
Lys Gln Ala Ile Ile Ala Gly Met Ala Ala Asp Ala Leu Gly Tyr Val           
285                 290                 295                 300           

ttg ctg gcg ttc gcg acg cga ggc tgg atg gcc ttc ccc att atg att       1024
Leu Leu Ala Phe Ala Thr Arg Gly Trp Met Ala Phe Pro Ile Met Ile           
                305                 310                 315               

ctt ctc gct tcc ggc ggc atc ggg atg ccc gcg ttg cag gcc atg ctg       1072
Leu Leu Ala Ser Gly Gly Ile Gly Met Pro Ala Leu Gln Ala Met Leu           
            320                 325                 330                   

tcc agg cag gta gat gac gac cat cag gga cag ctt caa gga tcg ctc       1120
Ser Arg Gln Val Asp Asp Asp His Gln Gly Gln Leu Gln Gly Ser Leu           
        335                 340                 345                       

gcg gct ctt acc agc cta act tcg atc act gga ccg ctg atc gtc acg       1168
Ala Ala Leu Thr Ser Leu Thr Ser Ile Thr Gly Pro Leu Ile Val Thr           
    350                 355                 360                           

gcg att tat gcc gcc tcg gcg agc aca tgg aac ggg ttg gca tgg att       1216
Ala Ile Tyr Ala Ala Ser Ala Ser Thr Trp Asn Gly Leu Ala Trp Ile           
365                 370                 375                 380           

gta ggc gcc gcc cta tac ctt gtc tgc ctc ccc gcg ttg cgt cgc ggt       1264
Val Gly Ala Ala Leu Tyr Leu Val Cys Leu Pro Ala Leu Arg Arg Gly           
                385                 390                 395               

gca tgg agc cgg gcc acc tcg acc tga                                   1291
Ala Trp Ser Arg Ala Thr Ser Thr                                           
            400                                                           


<210>  76
<211>  404
<212>  PRT
<213>  Escherichia coli

<400>  76

Met Lys Ser Asn Asn Ala Leu Ile Val Ile Leu Gly Thr Val Thr Leu 
1               5                   10                  15      


Asp Ala Val Gly Ile Gly Leu Val Met Pro Val Leu Pro Gly Leu Leu 
            20                  25                  30          


Arg Asp Ile Val His Ser Asp Ser Ile Ala Ser His Tyr Gly Val Leu 
        35                  40                  45              


Leu Ala Leu Tyr Ala Leu Met Gln Phe Leu Cys Ala Pro Val Leu Gly 
    50                  55                  60                  


Ala Leu Ser Asp Arg Phe Gly Arg Arg Pro Val Leu Leu Ala Ser Leu 
65                  70                  75                  80  


Leu Gly Ala Thr Ile Asp Tyr Ala Ile Met Ala Thr Thr Pro Val Leu 
                85                  90                  95      


Trp Ile Leu Tyr Ala Gly Arg Ile Val Ala Gly Ile Thr Gly Ala Thr 
            100                 105                 110         


Gly Ala Val Ala Gly Ala Tyr Ile Ala Asp Ile Thr Asp Gly Glu Asp 
        115                 120                 125             


Arg Ala Arg His Phe Gly Leu Met Ser Ala Cys Phe Gly Val Gly Met 
    130                 135                 140                 


Val Ala Gly Pro Val Ala Gly Gly Leu Leu Gly Ala Ile Ser Leu His 
145                 150                 155                 160 


Ala Pro Phe Leu Ala Ala Ala Val Leu Asn Gly Leu Asn Leu Leu Leu 
                165                 170                 175     


Gly Cys Phe Leu Met Gln Glu Ser His Lys Gly Glu Arg Arg Ser Gln 
            180                 185                 190         


Asn Tyr Pro Ile Val Arg Pro Met Pro Leu Arg Ala Phe Asn Pro Val 
        195                 200                 205             


Ser Ser Phe Arg Trp Ala Arg Gly Met Thr Ile Val Ala Ala Leu Met 
    210                 215                 220                 


Thr Val Phe Phe Ile Met Gln Leu Val Gly Gln Val Pro Ala Ala Leu 
225                 230                 235                 240 


Trp Val Ile Phe Gly Glu Asp Arg Phe Arg Trp Ser Ala Thr Met Ile 
                245                 250                 255     


Gly Leu Ser Leu Ala Val Phe Gly Ile Leu His Ala Leu Ala Gln Ala 
            260                 265                 270         


Phe Val Thr Gly Pro Ala Thr Lys Arg Phe Gly Glu Lys Gln Ala Ile 
        275                 280                 285             


Ile Ala Gly Met Ala Ala Asp Ala Leu Gly Tyr Val Leu Leu Ala Phe 
    290                 295                 300                 


Ala Thr Arg Gly Trp Met Ala Phe Pro Ile Met Ile Leu Leu Ala Ser 
305                 310                 315                 320 


Gly Gly Ile Gly Met Pro Ala Leu Gln Ala Met Leu Ser Arg Gln Val 
                325                 330                 335     


Asp Asp Asp His Gln Gly Gln Leu Gln Gly Ser Leu Ala Ala Leu Thr 
            340                 345                 350         


Ser Leu Thr Ser Ile Thr Gly Pro Leu Ile Val Thr Ala Ile Tyr Ala 
        355                 360                 365             


Ala Ser Ala Ser Thr Trp Asn Gly Leu Ala Trp Ile Val Gly Ala Ala 
    370                 375                 380                 


Leu Tyr Leu Val Cys Leu Pro Ala Leu Arg Arg Gly Ala Trp Ser Arg 
385                 390                 395                 400 


Ala Thr Ser Thr 
                


<210>  77
<211>  1010
<212>  DNA
<213>  Escherichia coli


<220>
<221>  gene
<222>  (1)..(1010)
<223>  Reverse complement sequence of the modified kanR gene (KanR55)

<400>  77
tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat       60

accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg      120

ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa      180

tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac      240

gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc      300

gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt      360

acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag      420

cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg      480

agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc      540

agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg      600

cgctgcctcg tcctgcagtt cattcagggc gtacacaggg ccctggaata aaacctcacc      660

ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc      720

ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca      780

agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc      840

tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat      900

ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga      960

cgtctgtcag accaagttta ctcgaacttc catatgaggc ctaaaaaaaa                1010


<210>  78
<211>  247
<212>  PRT
<213>  Herpes simplex virus


<220>
<221>  PEPTIDE
<222>  (1)..(247)
<223>  Human Herpes Simplex Virus protease domain (Q69527)

<400>  78

Met Ala Ser Ala Glu Met Arg Glu Arg Leu Glu Ala Pro Leu Pro Asp 
1               5                   10                  15      


Arg Ala Val Pro Ile Tyr Val Ala Gly Phe Leu Ala Leu Tyr Asp Ser 
            20                  25                  30          


Gly Asp Pro Gly Glu Leu Ala Leu Asp Pro Asp Thr Val Arg Ala Ala 
        35                  40                  45              


Leu Pro Pro Glu Asn Pro Leu Pro Ile Asn Val Asp His Arg Ala Arg 
    50                  55                  60                  


Cys Glu Val Gly Arg Val Leu Ala Val Val Asn Asp Pro Arg Gly Pro 
65                  70                  75                  80  


Phe Phe Val Gly Leu Ile Ala Cys Val Gln Leu Glu Arg Val Leu Glu 
                85                  90                  95      


Thr Ala Ala Ser Ala Ala Ile Phe Glu Arg Arg Gly Pro Ala Leu Ser 
            100                 105                 110         


Arg Glu Glu Arg Leu Leu Tyr Leu Ile Thr Asn Tyr Leu Pro Ser Val 
        115                 120                 125             


Ser Leu Ser Thr Lys Arg Arg Gly Asp Glu Val Pro Pro Asp Arg Thr 
    130                 135                 140                 


Leu Phe Ala His Val Ala Leu Cys Ala Ile Gly Arg Arg Leu Gly Thr 
145                 150                 155                 160 


Ile Val Thr Tyr Asp Thr Ser Leu Asp Ala Ala Ile Ala Pro Phe Arg 
                165                 170                 175     


His Leu Asp Pro Ala Thr Arg Glu Gly Val Arg Arg Glu Ala Ala Glu 
            180                 185                 190         


Ala Glu Leu Ala Leu Ala Gly Arg Thr Trp Ala Pro Gly Val Glu Ala 
        195                 200                 205             


Leu Thr His Thr Leu Leu Ser Thr Ala Val Asn Asn Met Met Leu Arg 
    210                 215                 220                 


Asp Arg Trp Ser Leu Val Ala Glu Arg Arg Arg Gln Ala Gly Ile Ala 
225                 230                 235                 240 


Gly His Thr Tyr Leu Gln Ala 
                245         


<210>  79
<211>  711
<212>  DNA
<213>  Anaplasma marginale


<220>
<221>  CDS
<222>  (1)..(711)
<223>  mCherry gene encoding mCherry fluorescent protein

<400>  79
atg gtc agc aag gga gag gaa gat aat atg gcg atc atc aaa gag ttt         48
Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe           
1               5                   10                  15                

atg aga ttt aag gtg cac atg gaa gga agc gtt aat ggt cat gag ttt         96
Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe           
            20                  25                  30                    

gaa atc gaa ggc gaa ggc gaa gga aga ccg tat gaa ggc aca cag acg        144
Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr           
        35                  40                  45                        

gct aaa ctt aag gtc aca aaa ggc gga ccg ctt cca ttc gcg tgg gat        192
Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp           
    50                  55                  60                            

att ctt tca ccg caa ttt atg tat ggt tct aaa gcc tat gtg aaa cat        240
Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His           
65                  70                  75                  80            

cct gcg gat att cct gac tac ctt aaa ctg tct ttc ccg gaa gga ttt        288
Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe           
                85                  90                  95                

aaa tgg gaa cgc gtc atg aac ttc gaa gat ggc ggc gtt gtt acg gtg        336
Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val           
            100                 105                 110                   

acg cag gat tca tca ctg caa gat gga gaa ttt att tat aaa gtt aaa        384
Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys           
        115                 120                 125                       

ctg cgc ggc aca aac ttt ccg tca gac gga cct gtc atg cag aag aaa        432
Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys           
    130                 135                 140                           

acg atg ggc tgg gaa gcc agc agc gag aga atg tac ccg gag gac gga        480
Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly           
145                 150                 155                 160           

gca ctt aaa ggc gaa atc aag caa cgc ctg aag ctg aaa gat gga ggc        528
Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly           
                165                 170                 175               

cat tat gat gcc gag gtc aag acg aca tac aaa gct aag aaa ccg gta        576
His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val           
            180                 185                 190                   

caa tta cct gga gca tac aac gtc aat atc aag ctg gat att acg tca        624
Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser           
        195                 200                 205                       

cat aat gaa gac tat acg att gta gag caa tat gaa aga gca gag gga        672
His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly           
    210                 215                 220                           

aga cac tct aca ggt gga atg gac gaa tta tac aaa tag                    711
Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys                           
225                 230                 235                               


<210>  80
<211>  236
<212>  PRT
<213>  Anaplasma marginale

<400>  80

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     


