                         SEQUENCE LISTING

<110>  Autodisplay Biotech GmbH
 
<120>  Autodisplay of antibodies

<130>  61871P EP

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  1344
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(1344)
<223>  DNA sequence of E.coli AIDA-I autotransporter, wild type

<220>
<221>  misc_feature
<222>  (1)..(489)
<223>  linker domain of AIDA-I

<220>
<221>  misc_feature
<222>  (490)..(1344)
<223>  transporter domain of AIDA-I

<400>  1
cttaatccta caaaagaaag tgcaggtaat actcttaccg tgtcaaatta tactgggaca       60

ccgggaagtg ttatttctct tggtggtgtg cttgaaggag ataattcact tacggaccgt      120

ctggtggtga aaggtaatac ctctggtcaa agtgacatcg tttacgtcaa tgaagatggc      180

agtggtggtc agacgagaga tggtattaat attatttctg tagagggaaa ttctgatgca      240

gaattctctc tgaagaaccg cgtagttgcc ggagcttatg attacacact gcagaaagga      300

aacgagagtg ggacagataa taagggatgg tatttaacca gtcatcttcc cacatctgat      360

acccggcaat acagaccgga gaacggaagt tatgctacca atatgacact ggctaactca      420

ctgttcctca tggatttgaa tgagcgtaag caattcaggg ccatgagtga taatacacag      480

cctgagtctg catccgtgtg gatgaggatc actggaggaa gaagctctgg taagcttaat      540

gacgggcaaa ataaaacaac aaccaatcag tttatcaatc agctcggggg ggatatttat      600

aaattccatg ctgaacaact gggtgatttt accttaggga ttatgggagg atacgcgaat      660

gcaaaaggta aaacgataaa ttacacgagc aacaaagctg ccagaaacac actggatggt      720

tattctgtcg gggtatacgg tacgtggtat cagaatgggg aaaatgcaac agggctcttt      780

gctgaaactt ggatgcaata taactggttt aatgcatcag tgaaaggtga cggactggaa      840

gaagaaaaat ataatctgaa tggtttaacc gcttctgcag gtgggggata taacctgaat      900

gtgcacacat ggacatcacc tgaaggaata acaggtgaat tctggttgca gcctcatttg      960

caggctgtct ggatgggggt tacaccggat acacatcagg aggataacgg aacggtggtg     1020

cagggagcag ggaaaaataa tattcagaca aaagcaggta ttcgtgcatc ctggaaggtg     1080

aaaagcaccc tggataagga taccgggcgg gagttcagtc cgtatataga ggcaaactgg     1140

atccataaca ctcatgaatt tggtgttaaa atgagtgatg acagccagtt gttgtcaggt     1200

agccgaaatc agggagagat aaagacaggt attgaagggg tgattactca aaacttgtca     1260

gtgaatggcg gagtcgcata tcaggcagga ggtcacggga gcaatgccat ctccggagca     1320

ctggggataa aatacagctt ctga                                            1344


<210>  2
<211>  447
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<222>  (1)..(447)
<223>  protein sequence of E.coli AIDA-I autotransporter

<220>
<221>  MISC_FEATURE
<222>  (1)..(163)
<223>  linker domain of AIDA-I

<220>
<221>  MISC_FEATURE
<222>  (164)..(447)
<223>  transporter domain of AIDA-I

<400>  2

Leu Asn Pro Thr Lys Glu Ser Ala Gly Asn Thr Leu Thr Val Ser Asn 
1               5                   10                  15      


Tyr Thr Gly Thr Pro Gly Ser Val Ile Ser Leu Gly Gly Val Leu Glu 
            20                  25                  30          


Gly Asp Asn Ser Leu Thr Asp Arg Leu Val Val Lys Gly Asn Thr Ser 
        35                  40                  45              


Gly Gln Ser Asp Ile Val Tyr Val Asn Glu Asp Gly Ser Gly Gly Gln 
    50                  55                  60                  


Thr Arg Asp Gly Ile Asn Ile Ile Ser Val Glu Gly Asn Ser Asp Ala 
65                  70                  75                  80  


Glu Phe Ser Leu Lys Asn Arg Val Val Ala Gly Ala Tyr Asp Tyr Thr 
                85                  90                  95      


Leu Gln Lys Gly Asn Glu Ser Gly Thr Asp Asn Lys Gly Trp Tyr Leu 
            100                 105                 110         


Thr Ser His Leu Pro Thr Ser Asp Thr Arg Gln Tyr Arg Pro Glu Asn 
        115                 120                 125             


Gly Ser Tyr Ala Thr Asn Met Thr Leu Ala Asn Ser Leu Phe Leu Met 
    130                 135                 140                 


Asp Leu Asn Glu Arg Lys Gln Phe Arg Ala Met Ser Asp Asn Thr Gln 
145                 150                 155                 160 


Pro Glu Ser Ala Ser Val Trp Met Arg Ile Thr Gly Gly Arg Ser Ser 
                165                 170                 175     


Gly Lys Leu Asn Asp Gly Gln Asn Lys Thr Thr Thr Asn Gln Phe Ile 
            180                 185                 190         


Asn Gln Leu Gly Gly Asp Ile Tyr Lys Phe His Ala Glu Gln Leu Gly 
        195                 200                 205             


Asp Phe Thr Leu Gly Ile Met Gly Gly Tyr Ala Asn Ala Lys Gly Lys 
    210                 215                 220                 


Thr Ile Asn Tyr Thr Ser Asn Lys Ala Ala Arg Asn Thr Leu Asp Gly 
225                 230                 235                 240 


Tyr Ser Val Gly Val Tyr Gly Thr Trp Tyr Gln Asn Gly Glu Asn Ala 
                245                 250                 255     


Thr Gly Leu Phe Ala Glu Thr Trp Met Gln Tyr Asn Trp Phe Asn Ala 
            260                 265                 270         


Ser Val Lys Gly Asp Gly Leu Glu Glu Glu Lys Tyr Asn Leu Asn Gly 
        275                 280                 285             


Leu Thr Ala Ser Ala Gly Gly Gly Tyr Asn Leu Asn Val His Thr Trp 
    290                 295                 300                 


Thr Ser Pro Glu Gly Ile Thr Gly Glu Phe Trp Leu Gln Pro His Leu 
305                 310                 315                 320 


Gln Ala Val Trp Met Gly Val Thr Pro Asp Thr His Gln Glu Asp Asn 
                325                 330                 335     


Gly Thr Val Val Gln Gly Ala Gly Lys Asn Asn Ile Gln Thr Lys Ala 
            340                 345                 350         


Gly Ile Arg Ala Ser Trp Lys Val Lys Ser Thr Leu Asp Lys Asp Thr 
        355                 360                 365             


Gly Arg Glu Phe Ser Pro Tyr Ile Glu Ala Asn Trp Ile His Asn Thr 
    370                 375                 380                 


His Glu Phe Gly Val Lys Met Ser Asp Asp Ser Gln Leu Leu Ser Gly 
385                 390                 395                 400 


Ser Arg Asn Gln Gly Glu Ile Lys Thr Gly Ile Glu Gly Val Ile Thr 
                405                 410                 415     


Gln Asn Leu Ser Val Asn Gly Gly Val Ala Tyr Gln Ala Gly Gly His 
            420                 425                 430         


Gly Ser Asn Ala Ile Ser Gly Ala Leu Gly Ile Lys Tyr Ser Phe 
        435                 440                 445         


<210>  3
<211>  489
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(489)
<223>  DNA sequence of E.coli AIDA-I linker domain, wild type

<400>  3
cttaatccta caaaagaaag tgcaggtaat actcttaccg tgtcaaatta tactgggaca       60

ccgggaagtg ttatttctct tggtggtgtg cttgaaggag ataattcact tacggaccgt      120

ctggtggtga aaggtaatac ctctggtcaa agtgacatcg tttacgtcaa tgaagatggc      180

agtggtggtc agacgagaga tggtattaat attatttctg tagagggaaa ttctgatgca      240

gaattctctc tgaagaaccg cgtagttgcc ggagcttatg attacacact gcagaaagga      300

aacgagagtg ggacagataa taagggatgg tatttaacca gtcatcttcc cacatctgat      360

acccggcaat acagaccgga gaacggaagt tatgctacca atatgacact ggctaactca      420

ctgttcctca tggatttgaa tgagcgtaag caattcaggg ccatgagtga taatacacag      480

cctgagtct                                                              489


<210>  4
<211>  163
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<222>  (1)..(163)
<223>  amino acid sequence of E.coli AIDA-I linker domain

<400>  4

Leu Asn Pro Thr Lys Glu Ser Ala Gly Asn Thr Leu Thr Val Ser Asn 
1               5                   10                  15      


Tyr Thr Gly Thr Pro Gly Ser Val Ile Ser Leu Gly Gly Val Leu Glu 
            20                  25                  30          


Gly Asp Asn Ser Leu Thr Asp Arg Leu Val Val Lys Gly Asn Thr Ser 
        35                  40                  45              


Gly Gln Ser Asp Ile Val Tyr Val Asn Glu Asp Gly Ser Gly Gly Gln 
    50                  55                  60                  


Thr Arg Asp Gly Ile Asn Ile Ile Ser Val Glu Gly Asn Ser Asp Ala 
65                  70                  75                  80  


Glu Phe Ser Leu Lys Asn Arg Val Val Ala Gly Ala Tyr Asp Tyr Thr 
                85                  90                  95      


Leu Gln Lys Gly Asn Glu Ser Gly Thr Asp Asn Lys Gly Trp Tyr Leu 
            100                 105                 110         


Thr Ser His Leu Pro Thr Ser Asp Thr Arg Gln Tyr Arg Pro Glu Asn 
        115                 120                 125             


Gly Ser Tyr Ala Thr Asn Met Thr Leu Ala Asn Ser Leu Phe Leu Met 
    130                 135                 140                 


Asp Leu Asn Glu Arg Lys Gln Phe Arg Ala Met Ser Asp Asn Thr Gln 
145                 150                 155                 160 


Pro Glu Ser 
            


<210>  5
<211>  855
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(855)
<223>  DNA sequence of E.coli AIDA-I transporter domain, wild type

<400>  5
gcatccgtgt ggatgaggat cactggagga agaagctctg gtaagcttaa tgacgggcaa       60

aataaaacaa caaccaatca gtttatcaat cagctcgggg gggatattta taaattccat      120

gctgaacaac tgggtgattt taccttaggg attatgggag gatacgcgaa tgcaaaaggt      180

aaaacgataa attacacgag caacaaagct gccagaaaca cactggatgg ttattctgtc      240

ggggtatacg gtacgtggta tcagaatggg gaaaatgcaa cagggctctt tgctgaaact      300

tggatgcaat ataactggtt taatgcatca gtgaaaggtg acggactgga agaagaaaaa      360

tataatctga atggtttaac cgcttctgca ggtgggggat ataacctgaa tgtgcacaca      420

tggacatcac ctgaaggaat aacaggtgaa ttctggttgc agcctcattt gcaggctgtc      480

tggatggggg ttacaccgga tacacatcag gaggataacg gaacggtggt gcagggagca      540

gggaaaaata atattcagac aaaagcaggt attcgtgcat cctggaaggt gaaaagcacc      600

ctggataagg ataccgggcg ggagttcagt ccgtatatag aggcaaactg gatccataac      660

actcatgaat ttggtgttaa aatgagtgat gacagccagt tgttgtcagg tagccgaaat      720

cagggagaga taaagacagg tattgaaggg gtgattactc aaaacttgtc agtgaatggc      780

ggagtcgcat atcaggcagg aggtcacggg agcaatgcca tctccggagc actggggata      840

aaatacagct tctga                                                       855


<210>  6
<211>  284
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<222>  (1)..(284)
<223>  amino acid sequence of E.coli AIDA-I transporter domain

<400>  6

Ala Ser Val Trp Met Arg Ile Thr Gly Gly Arg Ser Ser Gly Lys Leu 
1               5                   10                  15      


Asn Asp Gly Gln Asn Lys Thr Thr Thr Asn Gln Phe Ile Asn Gln Leu 
            20                  25                  30          


Gly Gly Asp Ile Tyr Lys Phe His Ala Glu Gln Leu Gly Asp Phe Thr 
        35                  40                  45              


Leu Gly Ile Met Gly Gly Tyr Ala Asn Ala Lys Gly Lys Thr Ile Asn 
    50                  55                  60                  


Tyr Thr Ser Asn Lys Ala Ala Arg Asn Thr Leu Asp Gly Tyr Ser Val 
65                  70                  75                  80  


Gly Val Tyr Gly Thr Trp Tyr Gln Asn Gly Glu Asn Ala Thr Gly Leu 
                85                  90                  95      


Phe Ala Glu Thr Trp Met Gln Tyr Asn Trp Phe Asn Ala Ser Val Lys 
            100                 105                 110         


Gly Asp Gly Leu Glu Glu Glu Lys Tyr Asn Leu Asn Gly Leu Thr Ala 
        115                 120                 125             


Ser Ala Gly Gly Gly Tyr Asn Leu Asn Val His Thr Trp Thr Ser Pro 
    130                 135                 140                 


Glu Gly Ile Thr Gly Glu Phe Trp Leu Gln Pro His Leu Gln Ala Val 
145                 150                 155                 160 


Trp Met Gly Val Thr Pro Asp Thr His Gln Glu Asp Asn Gly Thr Val 
                165                 170                 175     


Val Gln Gly Ala Gly Lys Asn Asn Ile Gln Thr Lys Ala Gly Ile Arg 
            180                 185                 190         


Ala Ser Trp Lys Val Lys Ser Thr Leu Asp Lys Asp Thr Gly Arg Glu 
        195                 200                 205             


Phe Ser Pro Tyr Ile Glu Ala Asn Trp Ile His Asn Thr His Glu Phe 
    210                 215                 220                 


Gly Val Lys Met Ser Asp Asp Ser Gln Leu Leu Ser Gly Ser Arg Asn 
225                 230                 235                 240 


Gln Gly Glu Ile Lys Thr Gly Ile Glu Gly Val Ile Thr Gln Asn Leu 
                245                 250                 255     


Ser Val Asn Gly Gly Val Ala Tyr Gln Ala Gly Gly His Gly Ser Asn 
            260                 265                 270         


Ala Ile Ser Gly Ala Leu Gly Ile Lys Tyr Ser Phe 
        275                 280                 


<210>  7
<211>  1467
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(612)
<223>  linker domain of EhaA

<220>
<221>  misc_feature
<222>  (1)..(1467)
<223>  DNA sequence of E.coli EhaA autotransporter, wild type

<220>
<221>  misc_feature
<222>  (613)..(1467)
<223>  transporter domain of EhaA

<400>  7
ctgaccaaca acggtacgct gatgaccgga atgtcaggac agcaagctgg caatgtgtta       60

gtggtgaagg ggaactacca cggtaataac ggtcaactag taatgaatac ggtactgaat      120

ggcgatgact cagtaaccga taaattggtt gtcgagggcg atactagcgg cacgactgcc      180

gttacggtga ataacgctgg cggtacaggt gcgaaaaccc ttaacggtat cgaacttatc      240

catgtagacg gtaagtctga gggcgaattt gttcaggctg ggcgtatcgt tgcgggggcg      300

tatgactaca ctctcgcgcg tggacaaggg gcaaatagtg gtaactggta tctgaccagc      360

ggcagtgatt ctcctgaact gcagccggag ccagacccga tgccgaatcc agagccaaac      420

ccgaatccag agccgaaccc taacccgaca cctacgccgg gtccggatct gaatgtggat      480

aatgacctgc gaccggaggc gggtagctac attgcgaacc ttgcagcagc gaataccatg      540

ttcaccacgc gtctgcatga gcgtctgggt aatacgtact ataccgacat ggtgacgggt      600

gagcagaaac aaaccactat gtggatgcgc catgaaggtg gtcataataa atggcgtgat      660

ggcagcggcc agctgaaaac ccaaagcaat cgctatgttc tgcaactggg aggcgatgtc      720

gcgcagtgga gccaaaacgg cagcgaccgc tggcatgttg gggtcatggc gggatatggc      780

aacagcgaca gcaaaaccat ttcctcgcga accggttatc gtgcaaaagc gagtgtgaac      840

ggatatagca caggcctcta tgccacctgg tatgccgatg acgagtcgcg taatggcgcg      900

tatctcgaca gttgggcgca gtacagctgg tttgataaca cagtgaaagg ggatgactta      960

caaagtgaat cctataaatc aaaaggattt accgcttcac tggaagctgg atacaaacac     1020

aaattagctg aatttaatgg cagccaggga acgcgtaatg aatggtatgt tcagccgcaa     1080

gcacaggtta cctggatggg agtcaaagcc gataagcacc gcgaaagcaa cggaaccctc     1140

gttcatagca acggtgatgg caatgttcaa acccgacttg gcgtaaaaac ctggctgaag     1200

agccaccata aaatggatga cggtaaatcc cgcgagttcc agccgtttgt agaagtgaac     1260

tggctacata acagtaagga tttcagcacc agtatggatg gcgtgtctgt cactcaggat     1320

ggagcccgaa atattgctga gataaaaacc ggggtggaag gacagctaaa tgccaacctg     1380

aatgtctggg ggaatgtggg cgttcaggtt gccgataggg gatataatga cacctctgca     1440

atggttggca ttaagtggca attctga                                         1467


<210>  8
<211>  1349
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<222>  (1)..(1349)
<223>  Protein sequence of E.coli EhaA autotransporter, 

       Escherichia coli, EhaA protein, accession number YP_003498036, 
       genbank identifier GI:291281218

<220>
<221>  MISC_FEATURE
<222>  (862)..(1065)
<223>  linker domain of EhaA

<220>
<221>  MISC_FEATURE
<222>  (1066)..(1439)
<223>  transporter domain of EhaA

<400>  8

Met Ala Phe Asn Ala Leu Leu Phe Met Gln Ser Trp Phe Tyr Leu Asp 
1               5                   10                  15      


Val Leu Leu Glu Ile Val Met Asn Lys Ile Tyr Arg Leu Lys Trp Asn 
            20                  25                  30          


Arg Ser Arg Asn Cys Trp Ser Val Cys Ser Glu Leu Gly Ser Arg Val 
        35                  40                  45              


Lys Gly Lys Lys Ser Arg Ala Val Leu Ile Ser Ala Ile Ser Leu Tyr 
    50                  55                  60                  


Ser Ser Leu Val Phe Ala Asp Asp Val Ile Val Asn Gln Asp Lys Thr 
65                  70                  75                  80  


Ile Asp Phe Gly Lys Glu Asn Gln Ser Ile Asp Tyr Arg Ile Thr Val 
                85                  90                  95      


Thr Asp Asn Ala Asn Leu Val Ile Asn Ala Thr Asp Thr Ser Arg Pro 
            100                 105                 110         


Arg Leu Thr Leu Ala Ser Gly Gly Gly Leu Asp Ile Thr Gly Gly Lys 
        115                 120                 125             


Val Thr Ile Asn Gly Pro Leu Asn Phe Leu Leu Lys Gly Thr Gly Phe 
    130                 135                 140                 


Leu Asn Val Ser Asn Ala Gly Ser Glu Leu Tyr Ala Asp Asp Leu Tyr 
145                 150                 155                 160 


Glu Ser Asn Ser Gly Met Arg His Asp Arg Gly Tyr Phe Asn Val Ser 
                165                 170                 175     


Asn Gly Gly Lys Ile His Val Lys Gly Thr Ser Arg Leu Thr Tyr Leu 
            180                 185                 190         


Gln Gly Asn Val Ser Gly Glu Gly Ser Gln Val Asn Ser Glu Thr Phe 
        195                 200                 205             


Phe Met Gly Val Tyr Gly Ser Tyr Gly Gly Asn Gln Tyr Leu Ser Val 
    210                 215                 220                 


Asn Asn Gly Gly Glu Val Asn Ala Arg Lys Gln Ile Ser Leu Gly Tyr 
225                 230                 235                 240 


Tyr Asp Gln Val Ser Asp Thr Thr Leu Ala Val Ser Glu Gly Gly Lys 
                245                 250                 255     


Ile Ser Ala Pro Thr Ile Ser Leu Ser Thr Asn Ser Glu Leu Ala Leu 
            260                 265                 270         


Gly Ala Gln Glu Gly Ser Ala Ala Lys Ala Ala Gly Ile Ile Asp Ala 
        275                 280                 285             


Glu Lys Ile Glu Phe Val Trp Ala Lys Thr Ser Glu Lys Lys Ile Thr 
    290                 295                 300                 


Leu Asn His Thr Asp Lys Asp Ala Thr Ile Ser Ala Asp Ile Val Ser 
305                 310                 315                 320 


Gly Ser Glu Gly Leu Gly Tyr Ile Asn Ala Leu Asn Gly Thr Thr Tyr 
                325                 330                 335     


Leu Thr Gly Asp Asn Ser Ala Phe Ser Gly Lys Val Lys Ile Glu Gln 
            340                 345                 350         


Asn Gly Ala Leu Gly Ile Thr Gln Asn Ile Gly Thr Ala Glu Ile Asn 
        355                 360                 365             


Asn Arg Gly Lys Leu His Leu Lys Ala Asp Asp Ser Met Thr Phe Ala 
    370                 375                 380                 


Asn Lys Ile Ser Gly Asn Gly Thr Ile Ser Ile Asp Ser Gly Thr Val 
385                 390                 395                 400 


Glu Leu Thr Gly Asn Asn Tyr Ala Phe Ser Gly Tyr Ile Asp Val Ala 
                405                 410                 415     


Ser Gly Ala Val Ala Val Ile Ser Glu Asp Lys Asn Ile Gly Arg Ala 
            420                 425                 430         


Glu Leu Asp Val Asp Gly Lys Leu Gln Ile Asn Ala Asn Lys Asp Trp 
        435                 440                 445             


Val Phe Asp Asn Asp Leu Glu Gly Arg Gly Ile Val Glu Ile Asn Met 
    450                 455                 460                 


Gly Asn His Glu Phe Ser Phe Asp Glu Phe Ala Tyr Thr Asp Trp Phe 
465                 470                 475                 480 


Gln Gly Ser Leu Ala Phe Gln Asn Thr Thr Phe Asn Leu Glu Lys Asn 
                485                 490                 495     


Ala Glu Phe Leu Gln Lys Gly Gly Ile Thr Ala Gly Gln Gly Ser Leu 
            500                 505                 510         


Val Thr Val Gly Lys Gly Ala His Ser Ile Ser Thr Leu Gly Phe Ser 
        515                 520                 525             


Gly Gly Thr Val Asp Phe Gly Ala Leu Thr Ala Gly Ala Gln Met Thr 
    530                 535                 540                 


Glu Gly Thr Val Asn Val Ser Lys Thr Leu Asp Leu Arg Gly Glu Gly 
545                 550                 555                 560 


Val Ile Gln Val Ser Asp Ser Asp Val Val Arg Ser Val Ser Arg Asp 
                565                 570                 575     


Ile Asp Ser Ala Leu Ser Leu Thr Glu Val Asp Asp Gly Asn Ser Thr 
            580                 585                 590         


Ile Lys Leu Val Asp Ala Gln Gly Ala Glu Val Leu Gly Asp Ala Gly 
        595                 600                 605             


Asn Leu Gln Leu Gln Asp Lys Asn Gly Gln Ile Leu Ser Ser Ser Ala 
    610                 615                 620                 


Gln Arg Asp Ile Gln Gln Asn Gly Gln Lys Ala Ala Val Gly Thr Tyr 
625                 630                 635                 640 


Asp Tyr Arg Leu Thr Ser Gly Val Asn Asn Asp Gly Leu Tyr Ile Gly 
                645                 650                 655     


Tyr Gly Leu Thr Gln Leu Asp Leu His Ala Thr Asp Ser Asp Ala Leu 
            660                 665                 670         


Val Leu Ser Ser Asn Gly Lys Ser Glu Asn Ala Ala Asp Leu Ser Ala 
        675                 680                 685             


Lys Ile Thr Gly Ser Gly Asp Leu Ala Phe Ser Ser Gln Lys Gly Gln 
    690                 695                 700                 


Thr Val Ser Leu Ser Asn Lys Asp Asn Asp Tyr Thr Gly Val Thr Asp 
705                 710                 715                 720 


Leu Arg Ser Gly Thr Leu Leu Leu Asn Asn Asp Asn Val Leu Gly Asn 
                725                 730                 735     


Thr His Glu Leu Arg Leu Ala Ala Glu Thr Glu Leu Asp Met Asn Gly 
            740                 745                 750         


His Ser Gln Thr Val Gly Thr Leu Asn Gly Ser Ala Asp Ser Leu Leu 
        755                 760                 765             


Ser Leu Asn Gly Gly Ser Leu Thr Val Thr Asn Gly Gly Thr Ser Thr 
    770                 775                 780                 


Gly Ser Leu Thr Gly Ser Gly Glu Leu Asn Ile Gln Gly Gly Thr Leu 
785                 790                 795                 800 


Asp Ile Ala Gly Asp Asn Ser Asn Leu Thr Ala Asn Val Asn Ile Ala 
                805                 810                 815     


Asn Ser Ala Asn Val Leu Val Ser His Ala Gln Gly Leu Gly Ser Ala 
            820                 825                 830         


Asn Val Glu Asn Asn Gly Thr Leu Ala Leu Asn Asn Ser Ala Glu Lys 
        835                 840                 845             


Arg Ala Ala Ala Ser Val Asn Tyr Ala Leu Gly Gly Asn Leu Thr Asn 
    850                 855                 860                 


Asn Gly Thr Leu Met Thr Gly Met Ser Gly Gln Gln Ala Gly Asn Val 
865                 870                 875                 880 


Leu Val Val Lys Gly Asn Tyr His Gly Asn Asn Gly Gln Leu Val Met 
                885                 890                 895     


Asn Thr Val Leu Asn Gly Asp Asp Ser Val Thr Asp Lys Leu Val Val 
            900                 905                 910         


Glu Gly Asp Thr Ser Gly Thr Thr Ala Val Thr Val Asn Asn Ala Gly 
        915                 920                 925             


Gly Thr Gly Ala Lys Thr Leu Asn Gly Ile Glu Leu Ile His Val Asp 
    930                 935                 940                 


Gly Lys Ser Glu Gly Glu Phe Val Gln Ala Gly Arg Ile Val Ala Gly 
945                 950                 955                 960 


Ala Tyr Asp Tyr Thr Leu Ala Arg Gly Gln Gly Ala Asn Ser Gly Asn 
                965                 970                 975     


Trp Tyr Leu Thr Ser Gly Ser Asp Ser Pro Glu Leu Gln Pro Glu Pro 
            980                 985                 990         


Asp Pro Met Pro Asn Pro Glu Pro  Asn Pro Asn Pro Glu  Pro Asn Pro 
        995                 1000                 1005             


Asn Pro  Thr Pro Thr Pro Gly  Pro Asp Leu Asn Val  Asp Asn Asp 
    1010                 1015                 1020             


Leu Arg  Pro Glu Ala Gly Ser  Tyr Ile Ala Asn Leu  Ala Ala Ala 
    1025                 1030                 1035             


Asn Thr  Met Phe Thr Thr Arg  Leu His Glu Arg Leu  Gly Asn Thr 
    1040                 1045                 1050             


Tyr Tyr  Thr Asp Met Val Thr  Gly Glu Gln Lys Gln  Thr Thr Met 
    1055                 1060                 1065             


Trp Met  Arg His Glu Gly Gly  His Asn Lys Trp Arg  Asp Gly Ser 
    1070                 1075                 1080             


Gly Gln  Leu Lys Thr Gln Ser  Asn Arg Tyr Val Leu  Gln Leu Gly 
    1085                 1090                 1095             


Gly Asp  Val Ala Gln Trp Ser  Gln Asn Gly Ser Asp  Arg Trp His 
    1100                 1105                 1110             


Val Gly  Val Met Ala Gly Tyr  Gly Asn Ser Asp Ser  Lys Thr Ile 
    1115                 1120                 1125             


Ser Ser  Arg Thr Gly Tyr Arg  Ala Lys Ala Ser Val  Asn Gly Tyr 
    1130                 1135                 1140             


Ser Thr  Gly Leu Tyr Ala Thr  Trp Tyr Ala Asp Asp  Glu Ser Arg 
    1145                 1150                 1155             


Asn Gly  Ala Tyr Leu Asp Ser  Trp Ala Gln Tyr Ser  Trp Phe Asp 
    1160                 1165                 1170             


Asn Thr  Val Lys Gly Asp Asp  Leu Gln Ser Glu Ser  Tyr Lys Ser 
    1175                 1180                 1185             


Lys Gly  Phe Thr Ala Ser Leu  Glu Ala Gly Tyr Lys  His Lys Leu 
    1190                 1195                 1200             


Ala Glu  Phe Asn Gly Ser Gln  Gly Thr Arg Asn Glu  Trp Tyr Val 
    1205                 1210                 1215             


Gln Pro  Gln Ala Gln Val Thr  Trp Met Gly Val Lys  Ala Asp Lys 
    1220                 1225                 1230             


His Arg  Glu Ser Asn Gly Thr  Leu Val His Ser Asn  Gly Asp Gly 
    1235                 1240                 1245             


Asn Val  Gln Thr Arg Leu Gly  Val Lys Thr Trp Leu  Lys Ser His 
    1250                 1255                 1260             


His Lys  Met Asp Asp Gly Lys  Ser Arg Glu Phe Gln  Pro Phe Val 
    1265                 1270                 1275             


Glu Val  Asn Trp Leu His Asn  Ser Lys Asp Phe Ser  Thr Ser Met 
    1280                 1285                 1290             


Asp Gly  Val Ser Val Thr Gln  Asp Gly Ala Arg Asn  Ile Ala Glu 
    1295                 1300                 1305             


Ile Lys  Thr Gly Val Glu Gly  Gln Leu Asn Ala Asn  Leu Asn Val 
    1310                 1315                 1320             


Trp Gly  Asn Val Gly Val Gln  Val Ala Asp Arg Gly  Tyr Asn Asp 
    1325                 1330                 1335             


Thr Ser  Ala Met Val Gly Ile  Lys Trp Gln Phe 
    1340                 1345                 


<210>  9
<211>  612
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(612)
<223>  DNA sequence of E.coli EhaA linker domain, wild type

<400>  9
ctgaccaaca acggtacgct gatgaccgga atgtcaggac agcaagctgg caatgtgtta       60

gtggtgaagg ggaactacca cggtaataac ggtcaactag taatgaatac ggtactgaat      120

ggcgatgact cagtaaccga taaattggtt gtcgagggcg atactagcgg cacgactgcc      180

gttacggtga ataacgctgg cggtacaggt gcgaaaaccc ttaacggtat cgaacttatc      240

catgtagacg gtaagtctga gggcgaattt gttcaggctg ggcgtatcgt tgcgggggcg      300

tatgactaca ctctcgcgcg tggacaaggg gcaaatagtg gtaactggta tctgaccagc      360

ggcagtgatt ctcctgaact gcagccggag ccagacccga tgccgaatcc agagccaaac      420

ccgaatccag agccgaaccc taacccgaca cctacgccgg gtccggatct gaatgtggat      480

aatgacctgc gaccggaggc gggtagctac attgcgaacc ttgcagcagc gaataccatg      540

ttcaccacgc gtctgcatga gcgtctgggt aatacgtact ataccgacat ggtgacgggt      600

gagcagaaac aa                                                          612


<210>  10
<211>  612
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of E.coli EhaA linker domain, codon optimized


<220>
<221>  misc_feature
<222>  (1)..(612)
<223>  DNA sequence of E.coli EhaA linker domain, codon optimized

<400>  10
ctgaccaaca atggcacgct gatgacgggt atgagcggtc aacaagcggg taacgttctg       60

gttgttaagg gcaattacca tggcaataac ggccagctgg tcatgaacac ggttctgaac      120

ggcgatgata gcgtgaccga caagctggtg gtcgagggcg acacctctgg tacgaccgca      180

gtgacggtga ataatgcagg cggtacgggt gccaaaaccc tgaacggtat tgagttgatc      240

cacgttgacg gtaagagcga gggcgagttt gtgcaggcag gccgcattgt tgctggcgct      300

tatgactata cgctggcccg tggtcagggc gcgaatagcg gtaactggta tctgaccagc      360

ggctccgact ccccggaact gcaaccggag cctgatccga tgccgaatcc ggagccaaac      420

ccgaacccgg aaccgaaccc aaatccgacc ccgactccgg gtccggactt gaacgttgat      480

aacgacctgc gtccggaggc cggttcgtac atcgcgaacc tggcagcggc caatacgatg      540

tttacgaccc gtctgcacga acgcctgggt aatacctact ataccgatat ggtcactggt      600

gaacagaaac aa                                                          612


<210>  11
<211>  204
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<222>  (1)..(204)
<223>  amino acid sequence of E.coli EhaA linker domain

<400>  11

Leu Thr Asn Asn Gly Thr Leu Met Thr Gly Met Ser Gly Gln Gln Ala 
1               5                   10                  15      


Gly Asn Val Leu Val Val Lys Gly Asn Tyr His Gly Asn Asn Gly Gln 
            20                  25                  30          


Leu Val Met Asn Thr Val Leu Asn Gly Asp Asp Ser Val Thr Asp Lys 
        35                  40                  45              


Leu Val Val Glu Gly Asp Thr Ser Gly Thr Thr Ala Val Thr Val Asn 
    50                  55                  60                  


Asn Ala Gly Gly Thr Gly Ala Lys Thr Leu Asn Gly Ile Glu Leu Ile 
65                  70                  75                  80  


His Val Asp Gly Lys Ser Glu Gly Glu Phe Val Gln Ala Gly Arg Ile 
                85                  90                  95      


Val Ala Gly Ala Tyr Asp Tyr Thr Leu Ala Arg Gly Gln Gly Ala Asn 
            100                 105                 110         


Ser Gly Asn Trp Tyr Leu Thr Ser Gly Ser Asp Ser Pro Glu Leu Gln 
        115                 120                 125             


Pro Glu Pro Asp Pro Met Pro Asn Pro Glu Pro Asn Pro Asn Pro Glu 
    130                 135                 140                 


Pro Asn Pro Asn Pro Thr Pro Thr Pro Gly Pro Asp Leu Asn Val Asp 
145                 150                 155                 160 


Asn Asp Leu Arg Pro Glu Ala Gly Ser Tyr Ile Ala Asn Leu Ala Ala 
                165                 170                 175     


Ala Asn Thr Met Phe Thr Thr Arg Leu His Glu Arg Leu Gly Asn Thr 
            180                 185                 190         


Tyr Tyr Thr Asp Met Val Thr Gly Glu Gln Lys Gln 
        195                 200                 


<210>  12
<211>  855
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(855)
<223>  DNA sequence of E.coli EhaA transporter domain, wild type

<400>  12
accactatgt ggatgcgcca tgaaggtggt cataataaat ggcgtgatgg cagcggccag       60

ctgaaaaccc aaagcaatcg ctatgttctg caactgggag gcgatgtcgc gcagtggagc      120

caaaacggca gcgaccgctg gcatgttggg gtcatggcgg gatatggcaa cagcgacagc      180

aaaaccattt cctcgcgaac cggttatcgt gcaaaagcga gtgtgaacgg atatagcaca      240

ggcctctatg ccacctggta tgccgatgac gagtcgcgta atggcgcgta tctcgacagt      300

tgggcgcagt acagctggtt tgataacaca gtgaaagggg atgacttaca aagtgaatcc      360

tataaatcaa aaggatttac cgcttcactg gaagctggat acaaacacaa attagctgaa      420

tttaatggca gccagggaac gcgtaatgaa tggtatgttc agccgcaagc acaggttacc      480

tggatgggag tcaaagccga taagcaccgc gaaagcaacg gaaccctcgt tcatagcaac      540

ggtgatggca atgttcaaac ccgacttggc gtaaaaacct ggctgaagag ccaccataaa      600

atggatgacg gtaaatcccg cgagttccag ccgtttgtag aagtgaactg gctacataac      660

agtaaggatt tcagcaccag tatggatggc gtgtctgtca ctcaggatgg agcccgaaat      720

attgctgaga taaaaaccgg ggtggaagga cagctaaatg ccaacctgaa tgtctggggg      780

aatgtgggcg ttcaggttgc cgatagggga tataatgaca cctctgcaat ggttggcatt      840

aagtggcaat tctga                                                       855


<210>  13
<211>  855
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of E.coli EhaAI transporter domain, codon optimized


<220>
<221>  misc_feature
<222>  (1)..(855)
<223>  DNA sequence of E.coli EhaA transporter domain, codon optimized

<400>  13
accaccatgt ggatgcgcca cgagggtggt cacaataagt ggcgcgacgg tagcggccag       60

ttgaaaaccc agagcaatcg ctacgttctg caattgggcg gtgatgtggc gcaatggagc      120

caaaacggca gcgaccgttg gcatgtcggt gtgatggcag gttacggcaa cagcgacagc      180

aagaccatct ccagccgtac cggttaccgt gcgaaggcaa gcgtcaacgg ttacagcacc      240

ggcctgtatg ccacctggta tgctgatgat gagagccgca acggtgctta cttggacagc      300

tgggcacagt attcttggtt cgataatacg gtgaaaggcg acgacctgca gagcgaaagc      360

tacaaatcga aaggtttcac cgcgagcctg gaagccggct ataagcacaa actggcggaa      420

ttcaatggca gccagggtac tcgtaacgaa tggtacgttc aaccgcaggc gcaagtcact      480

tggatgggcg ttaaggcgga taaacaccgt gagagcaacg gtacgttggt gcatagcaac      540

ggtgatggta atgtccaaac ccgtctgggt gtgaaaacgt ggctgaagtc ccatcacaaa      600

atggacgacg gtaaatctcg tgaatttcag ccgttcgtgg aagttaactg gctgcataat      660

agcaaggatt tcagcacgag catggatggt gtctccgtta cccaggacgg cgcacgtaac      720

attgcggaga tcaagaccgg cgtcgagggt cagctgaatg cgaatctgaa tgtttggggt      780

aacgtgggtg ttcaagtagc ggaccgtggt tacaatgata ccagcgcgat ggtgggtatt      840

aagtggcagt tttaa                                                       855


<210>  14
<211>  284
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<222>  (1)..(284)
<223>  amino acid sequence of E.coli EhaA transporter domain

<400>  14

Thr Thr Met Trp Met Arg His Glu Gly Gly His Asn Lys Trp Arg Asp 
1               5                   10                  15      


Gly Ser Gly Gln Leu Lys Thr Gln Ser Asn Arg Tyr Val Leu Gln Leu 
            20                  25                  30          


Gly Gly Asp Val Ala Gln Trp Ser Gln Asn Gly Ser Asp Arg Trp His 
        35                  40                  45              


Val Gly Val Met Ala Gly Tyr Gly Asn Ser Asp Ser Lys Thr Ile Ser 
    50                  55                  60                  


Ser Arg Thr Gly Tyr Arg Ala Lys Ala Ser Val Asn Gly Tyr Ser Thr 
65                  70                  75                  80  


Gly Leu Tyr Ala Thr Trp Tyr Ala Asp Asp Glu Ser Arg Asn Gly Ala 
                85                  90                  95      


Tyr Leu Asp Ser Trp Ala Gln Tyr Ser Trp Phe Asp Asn Thr Val Lys 
            100                 105                 110         


Gly Asp Asp Leu Gln Ser Glu Ser Tyr Lys Ser Lys Gly Phe Thr Ala 
        115                 120                 125             


Ser Leu Glu Ala Gly Tyr Lys His Lys Leu Ala Glu Phe Asn Gly Ser 
    130                 135                 140                 


Gln Gly Thr Arg Asn Glu Trp Tyr Val Gln Pro Gln Ala Gln Val Thr 
145                 150                 155                 160 


Trp Met Gly Val Lys Ala Asp Lys His Arg Glu Ser Asn Gly Thr Leu 
                165                 170                 175     


Val His Ser Asn Gly Asp Gly Asn Val Gln Thr Arg Leu Gly Val Lys 
            180                 185                 190         


Thr Trp Leu Lys Ser His His Lys Met Asp Asp Gly Lys Ser Arg Glu 
        195                 200                 205             


Phe Gln Pro Phe Val Glu Val Asn Trp Leu His Asn Ser Lys Asp Phe 
    210                 215                 220                 


Ser Thr Ser Met Asp Gly Val Ser Val Thr Gln Asp Gly Ala Arg Asn 
225                 230                 235                 240 


Ile Ala Glu Ile Lys Thr Gly Val Glu Gly Gln Leu Asn Ala Asn Leu 
                245                 250                 255     


Asn Val Trp Gly Asn Val Gly Val Gln Val Ala Asp Arg Gly Tyr Asn 
            260                 265                 270         


Asp Thr Ser Ala Met Val Gly Ile Lys Trp Gln Phe 
        275                 280                 


<210>  15
<211>  2094
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of  fusion construct comprising a signal peptide, 
       the light chain and the AIDA-I autotransporter (linker domain and
       autotransporter domain)


<220>
<221>  misc_feature
<222>  (1)..(87)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (88)..(750)
<223>  light chain T84.66

<220>
<221>  misc_feature
<222>  (751)..(2094)
<223>  AIDA-I autotransporter: linker domain and transporter domain

<400>  15
atggttaaat taaaatttgg tgtttttttt acagttttac tatcttcagc atatgcacat       60

ggaacacctc aaaatattac tgatttgctc gaggatattg ttctgaccca gagtccggca      120

agcctggcag ttagcctggg tcagcgtgca accatgagct gtcgtgccgg tgaaagcgtt      180

gatatttttg gtgttggttt tctgcattgg tatcagcaga aaccgggtca gcctccgaaa      240

ctgctgattt atcgtgcaag caatctggaa agcggtattc cggttcgttt tagcggcacc      300

ggtagccgta ccgattttac cctgattatt gatccggttg aagcagatga tgttgccacc      360

tattattgtc agcagaccaa tgaagatccg tatacctttg gtggtggcac caaactggaa      420

atcaaaaccg ttgcagcacc gagcgttttt atctttccgc ctagtgatga acagctgaaa      480

agcggtacag caagcgttgt ttgtctgctg aataactttt atccgcgtga agcaaaagtt      540

cagtggaaag ttgataatgc actgcagagc ggtaatagcc aagaaagcgt taccgaacag      600

gatagcaaag atagcaccta tagcctgagc agtaccctga ccctgagcaa agcagattat      660

gaaaaacaca aagtgtatgc ctgcgaagtt acccatcagg gtctgagcag tccggttacc      720

aaaagcttta atcgtggtga atgtggtacc cttaatccta caaaagaaag tgcaggtaat      780

actcttaccg tgtcaaatta tactgggaca ccgggaagtg ttatttctct tggtggtgtg      840

cttgaaggag ataattcact tacggaccgt ctggtggtga aaggtaatac ctctggtcaa      900

agtgacatcg tttatgtcaa tgaagatggc agtggtggtc agacgagaga tggtattaat      960

attatttctg tagagggaaa ttctgatgca gaattctctc tgaagaaccg cgtagttgcc     1020

ggagcttatg attacacact gcagaaagga aacgagagtg ggacagataa taagggatgg     1080

tatttaacca gtcatcttcc cacatctgat acccggcaat acagaccgga gaacggaagt     1140

tatgctacca atatggcact ggctaactca ctgttcctca tggatttgaa tgagcgtaag     1200

caattcaggg ccatgagtga taatacacag cctgagtctg catccgtgtg gatgaagatc     1260

actggaggaa taagctctgg taagcttaat gacgggcaaa ataaaacaac aaccaatcag     1320

tttatcaatc agctcggggg ggatatttat aaattccatg ctgaacaact gggtgatttt     1380

accttaggga ttatgggagg atacgcgaat gcaaaaggta aaacgataaa ttacacgagc     1440

aacaaagctg ccagaaacac actggatggt tattctgtcg gggtatacgg tacgtggtat     1500

cagaatgggg aaaatgcaac agggctcttt gctgaaactt ggatgcaata taactggttt     1560

aatgcatcag tgaaaggtga cggactggaa gaagaaaaat ataatctgaa tggtttaacc     1620

gcttctgcag gtgggggata taacctgaat gtgcacacat ggacatcacc tgaaggaata     1680

acaggtgaat tctggttaca gcctcatttg caggctgtct ggatgggggt tacaccggat     1740

acacatcagg aggataacgg aacggtggtg cagggagcag ggaaaaataa tattcagaca     1800

aaagcaggta ttcgtgcatc ctggaaggtg aaaagcaccc tggataagga taccgggcgg     1860

aggttccgtc cgtatataga ggcaaactgg atccataaca ctcatgaatt tggtgttaaa     1920

atgagtgatg acagccagtt gttgtcaggt agccgaaatc agggagagat aaagacaggt     1980

attgaagggg tgattactca aaacttgtca gtgaatggcg gagtcgcata tcaggcagga     2040

ggtcacggga gcaatgccat ctccggagca ctggggataa aatacagctt ctga           2094


<210>  16
<211>  695
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of  fusion construct comprising a signal 
       peptide, the light chain and the AIDA-I autotransporter (linker 
       domain and autotransporter domain)


<220>
<221>  MISC_FEATURE
<222>  (1)..(29)
<223>  signal peptide

<220>
<221>  MISC_FEATURE
<222>  (30)..(250)
<223>  light chain T84.66

<220>
<221>  MISC_FEATURE
<222>  (251)..(695)
<223>  AIDA-I autotransporter: linker domain and transporter domain

<400>  16

Met Val Lys Leu Lys Phe Gly Val Phe Phe Thr Val Leu Leu Ser Ser 
1               5                   10                  15      


Ala Tyr Ala His Gly Thr Pro Gln Asn Ile Thr Asp Leu Leu Glu Asp 
            20                  25                  30          


Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln 
        35                  40                  45              


Arg Ala Thr Met Ser Cys Arg Ala Gly Glu Ser Val Asp Ile Phe Gly 
    50                  55                  60                  


Val Gly Phe Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 
65                  70                  75                  80  


Leu Leu Ile Tyr Arg Ala Ser Asn Leu Glu Ser Gly Ile Pro Val Arg 
                85                  90                  95      


Phe Ser Gly Thr Gly Ser Arg Thr Asp Phe Thr Leu Ile Ile Asp Pro 
            100                 105                 110         


Val Glu Ala Asp Asp Val Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Glu 
        115                 120                 125             


Asp Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Thr Val 
    130                 135                 140                 


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
145                 150                 155                 160 


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
                165                 170                 175     


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
            180                 185                 190         


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
        195                 200                 205             


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
    210                 215                 220                 


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
225                 230                 235                 240 


Lys Ser Phe Asn Arg Gly Glu Cys Gly Thr Leu Asn Pro Thr Lys Glu 
                245                 250                 255     


Ser Ala Gly Asn Thr Leu Thr Val Ser Asn Tyr Thr Gly Thr Pro Gly 
            260                 265                 270         


Ser Val Ile Ser Leu Gly Gly Val Leu Glu Gly Asp Asn Ser Leu Thr 
        275                 280                 285             


Asp Arg Leu Val Val Lys Gly Asn Thr Ser Gly Gln Ser Asp Ile Val 
    290                 295                 300                 


Tyr Val Asn Glu Asp Gly Ser Gly Gly Gln Thr Arg Asp Gly Ile Asn 
305                 310                 315                 320 


Ile Ile Ser Val Glu Gly Asn Ser Asp Ala Glu Phe Ser Leu Lys Asn 
                325                 330                 335     


Arg Val Val Ala Gly Ala Tyr Asp Tyr Thr Leu Gln Lys Gly Asn Glu 
            340                 345                 350         


Ser Gly Thr Asp Asn Lys Gly Trp Tyr Leu Thr Ser His Leu Pro Thr 
        355                 360                 365             


Ser Asp Thr Arg Gln Tyr Arg Pro Glu Asn Gly Ser Tyr Ala Thr Asn 
    370                 375                 380                 


Met Ala Leu Ala Asn Ser Leu Phe Leu Met Asp Leu Asn Glu Arg Lys 
385                 390                 395                 400 


Gln Phe Arg Ala Met Ser Asp Asn Thr Gln Pro Glu Ser Ala Ser Val 
                405                 410                 415     


Trp Met Lys Ile Thr Gly Gly Ile Ser Ser Gly Lys Leu Asn Asp Gly 
            420                 425                 430         


Gln Asn Lys Thr Thr Thr Asn Gln Phe Ile Asn Gln Leu Gly Gly Asp 
        435                 440                 445             


Ile Tyr Lys Phe His Ala Glu Gln Leu Gly Asp Phe Thr Leu Gly Ile 
    450                 455                 460                 


Met Gly Gly Tyr Ala Asn Ala Lys Gly Lys Thr Ile Asn Tyr Thr Ser 
465                 470                 475                 480 


Asn Lys Ala Ala Arg Asn Thr Leu Asp Gly Tyr Ser Val Gly Val Tyr 
                485                 490                 495     


Gly Thr Trp Tyr Gln Asn Gly Glu Asn Ala Thr Gly Leu Phe Ala Glu 
            500                 505                 510         


Thr Trp Met Gln Tyr Asn Trp Phe Asn Ala Ser Val Lys Gly Asp Gly 
        515                 520                 525             


Leu Glu Glu Glu Lys Tyr Asn Leu Asn Gly Leu Thr Ala Ser Ala Gly 
    530                 535                 540                 


Gly Gly Tyr Asn Leu Asn Val His Thr Trp Thr Ser Pro Glu Gly Ile 
545                 550                 555                 560 


Thr Gly Glu Phe Trp Leu Gln Pro His Leu Gln Ala Val Trp Met Gly 
                565                 570                 575     


Val Thr Pro Asp Thr His Gln Glu Asp Asn Gly Thr Val Val Gln Gly 
            580                 585                 590         


Ala Gly Lys Asn Asn Ile Gln Thr Lys Ala Gly Ile Arg Ala Ser Trp 
        595                 600                 605             


Lys Val Lys Ser Thr Leu Asp Lys Asp Thr Gly Arg Arg Phe Arg Pro 
    610                 615                 620                 


Tyr Ile Glu Ala Asn Trp Ile His Asn Thr His Glu Phe Gly Val Lys 
625                 630                 635                 640 


Met Ser Asp Asp Ser Gln Leu Leu Ser Gly Ser Arg Asn Gln Gly Glu 
                645                 650                 655     


Ile Lys Thr Gly Ile Glu Gly Val Ile Thr Gln Asn Leu Ser Val Asn 
            660                 665                 670         


Gly Gly Val Ala Tyr Gln Ala Gly Gly His Gly Ser Asn Ala Ile Ser 
        675                 680                 685             


Gly Ala Leu Gly Ile Lys Tyr 
    690                 695 


<210>  17
<211>  2784
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of  fusion construct comprising a signal peptide, 
       the heavy chain and the AIDA-I autotransporter (linker domain and
       autotransporter domain)


<220>
<221>  misc_feature
<222>  (1)..(66)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (67)..(1440)
<223>  heavy chain T84.66

<220>
<221>  misc_feature
<222>  (1441)..(2784)
<223>  AIDA-I autotransporter: linker domain and transporter domain

<400>  17
atggttaaat taaaatttgg tgtttttttt acagttttac tatcttcagc atatgcacat       60

ggaacactcg aggaagttca gctgcagcag agcggtgcag aactggttga accgggtgca      120

agcgttaaac tgagctgtac cgcaagcggt tttaacatca aagataccta tatgcattgg      180

gtgaaacagc gtccggaaca gggtctggaa tggattggtc gtattgatcc ggcaaatggc      240

aatagcaaat atgtgccgaa atttcagggt aaagcaacca ttaccgcaga taccagcagc      300

aataccgcat atctgcaact gaccagcctg accagtgaag ataccgcagt ttattactgt      360

gcaccgtttg gttattacgt tagcgattat gcaatggcat attggggtca gggcaccagc      420

gttaccgtta gcagcgcaag caccaaaggt ccgagcgttt ttccgctggc accgagcagc      480

aaaagcacca gcggtggcac cgcagcactg ggttgtctgg ttaaagatta ttttccggaa      540

ccggttaccg tgagctggaa tagcggtgcg ctgacctcag gtgttcatac ctttccggca      600

gtgctgcaga gcagcggtct gtatagcctg agcagcgttg tgaccgttcc gagcagtagc      660

ctgggcaccc agacctatat ttgtaatgtt aatcataaac cgagcaacac caaagtggat      720

aaaaaagtgg aaccgaaaag cccgaaaagt tgcgataaaa cccatacctg tccgccttgt      780

ccggcaccgg aactgctggg tggtccgtca gtttttctgt ttccgcctaa accgaaagat      840

accctgatga ttagccgtac accggaagtt acctgtgttg ttgttgatgt tagccatgaa      900

gatccggaag tgaaatttaa ctggtatgtt gatggtgtgg aagtgcataa tgcaaaaacc      960

aaaccgcgtg aagaacagta taattcaacc tatcgtgttg ttagcgtgct gaccgttctg     1020

catcaggatt ggctgaatgg taaagaatac aaatgcaaag tgagcaacaa agcactgcct     1080

gcaccgattg aaaaaaccat tagcaaagca aaaggtcagc ctcgtgaacc gcaggtttat     1140

accctgcctc cgagccgtga tgaactgacc aaaaatcagg ttagcctgac ctgtctggtg     1200

aaaggttttt atccgagcga tattgcagtt gaatgggaaa gcaatggtca gccggaaaac     1260

aactataaaa ccacccctcc ggttctggat agtgatggta gttttttcct gtatagcaaa     1320

ctgaccgttg ataaaagccg ttggcagcag ggtaatgttt ttagctgtag cgttatgcat     1380

gaagccctgc ataatcatta tacccagaaa agcctgagcc tgagtccggg taaaggtacc     1440

cttaatccta caaaagaaag tgcaggtaat actcttaccg tgtcaaatta tactgggaca     1500

ccgggaagtg ttatttctct tggtggtgtg cttgaaggag ataattcact tacggaccgt     1560

ctggtggtga aaggtaatac ctctggtcaa agtgacatcg tttatgtcaa tgaagatggc     1620

agtggtggtc agacgagaga tggtattaat attatttctg tagagggaaa ttctgatgca     1680

gaattctctc tgaagaaccg cgtagttgcc ggagcttatg attacacact gcagaaagga     1740

aacgagagtg ggacagataa taagggatgg tatttaacca gtcatcttcc cacatctgat     1800

acccggcaat acagaccgga gaacggaagt tatgctacca atatggcact ggctaactca     1860

ctgttcctca tggatttgaa tgagcgtaag caattcaggg ccatgagtga taatacacag     1920

cctgagtctg catccgtgtg gatgaagatc actggaggaa taagctctgg taagcttaat     1980

gacgggcaaa ataaaacaac aaccaatcag tttatcaatc agctcggggg ggatatttat     2040

aaattccatg ctgaacaact gggtgatttt accttaggga ttatgggagg atacgcgaat     2100

gcaaaaggta aaacgataaa ttacacgagc aacaaagctg ccagaaacac actggatggt     2160

tattctgtcg gggtatacgg tacgtggtat cagaatgggg aaaatgcaac agggctcttt     2220

gctgaaactt ggatgcaata taactggttt aatgcatcag tgaaaggtga cggactggaa     2280

gaagaaaaat ataatctgaa tggtttaacc gcttctgcag gtgggggata taacctgaat     2340

gtgcacacat ggacatcacc tgaaggaata acaggtgaat tctggttaca gcctcatttg     2400

caggctgtct ggatgggggt tacaccggat acacatcagg aggataacgg aacggtggtg     2460

cagggagcag ggaaaaataa tattcagaca aaagcaggta ttcgtgcatc ctggaaggtg     2520

aaaagcaccc tggataagga taccgggcgg aggttccgtc cgtatataga ggcaaactgg     2580

atccataaca ctcatgaatt tggtgttaaa atgagtgatg acagccagtt gttgtcaggt     2640

agccgaaatc agggagagat aaagacaggt attgaagggg tgattactca aaacttgtca     2700

gtgaatggcg gagtcgcata tcaggcagga ggtcacggga gcaatgccat ctccggagca     2760

ctggggataa aatacagctt ctga                                            2784


<210>  18
<211>  927
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of  fusion constructcomprising a signal 
       peptide, the heavy chain and the AIDA-I autotransporter (linker 
       domain and autotransporter domain)


<220>
<221>  MISC_FEATURE
<222>  (1)..(22)
<223>  signal peptide

<220>
<221>  MISC_FEATURE
<222>  (23)..(480)
<223>  heavy chain T84.66

<220>
<221>  MISC_FEATURE
<222>  (481)..(927)
<223>  AIDA-I autotransporter: linker domain and transporter domain

<400>  18

Met Val Lys Leu Lys Phe Gly Val Phe Phe Thr Val Leu Leu Ser Ser 
1               5                   10                  15      


Ala Tyr Ala His Gly Thr Leu Glu Glu Val Gln Leu Gln Gln Ser Gly 
            20                  25                  30          


Ala Glu Leu Val Glu Pro Gly Ala Ser Val Lys Leu Ser Cys Thr Ala 
        35                  40                  45              


Ser Gly Phe Asn Ile Lys Asp Thr Tyr Met His Trp Val Lys Gln Arg 
    50                  55                  60                  


Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile Asp Pro Ala Asn Gly 
65                  70                  75                  80  


Asn Ser Lys Tyr Val Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr Ala 
                85                  90                  95      


Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu Thr Ser Leu Thr Ser 
            100                 105                 110         


Glu Asp Thr Ala Val Tyr Tyr Cys Ala Pro Phe Gly Tyr Tyr Val Ser 
        115                 120                 125             


Asp Tyr Ala Met Ala Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
    130                 135                 140                 


Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
145                 150                 155                 160 


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
                165                 170                 175     


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
            180                 185                 190         


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
        195                 200                 205             


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
    210                 215                 220                 


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
225                 230                 235                 240 


Lys Lys Val Glu Pro Lys Ser Pro Lys Ser Cys Asp Lys Thr His Thr 
                245                 250                 255     


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
            260                 265                 270         


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
        275                 280                 285             


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
    290                 295                 300                 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
305                 310                 315                 320 


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
                325                 330                 335     


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
            340                 345                 350         


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
        355                 360                 365             


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
    370                 375                 380                 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
385                 390                 395                 400 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
                405                 410                 415     


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
            420                 425                 430         


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
        435                 440                 445             


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
    450                 455                 460                 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Thr 
465                 470                 475                 480 


Leu Asn Pro Thr Lys Glu Ser Ala Gly Asn Thr Leu Thr Val Ser Asn 
                485                 490                 495     


Tyr Thr Gly Thr Pro Gly Ser Val Ile Ser Leu Gly Gly Val Leu Glu 
            500                 505                 510         


Gly Asp Asn Ser Leu Thr Asp Arg Leu Val Val Lys Gly Asn Thr Ser 
        515                 520                 525             


Gly Gln Ser Asp Ile Val Tyr Val Asn Glu Asp Gly Ser Gly Gly Gln 
    530                 535                 540                 


Thr Arg Asp Gly Ile Asn Ile Ile Ser Val Glu Gly Asn Ser Asp Ala 
545                 550                 555                 560 


Glu Phe Ser Leu Lys Asn Arg Val Val Ala Gly Ala Tyr Asp Tyr Thr 
                565                 570                 575     


Leu Gln Lys Gly Asn Glu Ser Gly Thr Asp Asn Lys Gly Trp Tyr Leu 
            580                 585                 590         


Thr Ser His Leu Pro Thr Ser Asp Thr Arg Gln Tyr Arg Pro Glu Asn 
        595                 600                 605             


Gly Ser Tyr Ala Thr Asn Met Ala Leu Ala Asn Ser Leu Phe Leu Met 
    610                 615                 620                 


Asp Leu Asn Glu Arg Lys Gln Phe Arg Ala Met Ser Asp Asn Thr Gln 
625                 630                 635                 640 


Pro Glu Ser Ala Ser Val Trp Met Lys Ile Thr Gly Gly Ile Ser Ser 
                645                 650                 655     


Gly Lys Leu Asn Asp Gly Gln Asn Lys Thr Thr Thr Asn Gln Phe Ile 
            660                 665                 670         


Asn Gln Leu Gly Gly Asp Ile Tyr Lys Phe His Ala Glu Gln Leu Gly 
        675                 680                 685             


Asp Phe Thr Leu Gly Ile Met Gly Gly Tyr Ala Asn Ala Lys Gly Lys 
    690                 695                 700                 


Thr Ile Asn Tyr Thr Ser Asn Lys Ala Ala Arg Asn Thr Leu Asp Gly 
705                 710                 715                 720 


Tyr Ser Val Gly Val Tyr Gly Thr Trp Tyr Gln Asn Gly Glu Asn Ala 
                725                 730                 735     


Thr Gly Leu Phe Ala Glu Thr Trp Met Gln Tyr Asn Trp Phe Asn Ala 
            740                 745                 750         


Ser Val Lys Gly Asp Gly Leu Glu Glu Glu Lys Tyr Asn Leu Asn Gly 
        755                 760                 765             


Leu Thr Ala Ser Ala Gly Gly Gly Tyr Asn Leu Asn Val His Thr Trp 
    770                 775                 780                 


Thr Ser Pro Glu Gly Ile Thr Gly Glu Phe Trp Leu Gln Pro His Leu 
785                 790                 795                 800 


Gln Ala Val Trp Met Gly Val Thr Pro Asp Thr His Gln Glu Asp Asn 
                805                 810                 815     


Gly Thr Val Val Gln Gly Ala Gly Lys Asn Asn Ile Gln Thr Lys Ala 
            820                 825                 830         


Gly Ile Arg Ala Ser Trp Lys Val Lys Ser Thr Leu Asp Lys Asp Thr 
        835                 840                 845             


Gly Arg Arg Phe Arg Pro Tyr Ile Glu Ala Asn Trp Ile His Asn Thr 
    850                 855                 860                 


His Glu Phe Gly Val Lys Met Ser Asp Asp Ser Gln Leu Leu Ser Gly 
865                 870                 875                 880 


Ser Arg Asn Gln Gly Glu Ile Lys Thr Gly Ile Glu Gly Val Ile Thr 
                885                 890                 895     


Gln Asn Leu Ser Val Asn Gly Gly Val Ala Tyr Gln Ala Gly Gly His 
            900                 905                 910         


Gly Ser Asn Ala Ile Ser Gly Ala Leu Gly Ile Lys Tyr Ser Phe 
        915                 920                 925         


<210>  19
<211>  2199
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of  fusion construct comprising a signal peptide, 
       the light chain and the EhaA autotransporter (linker domain and 
       autotransporter domain)


<220>
<221>  misc_feature
<222>  (1)..(81)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (82)..(732)
<223>  light chain T84.66

<220>
<221>  misc_feature
<222>  (733)..(2199)
<223>  EhaA autotransporter: linker domain and transporter domain

<400>  19
atgatcaaac tgaaattcgg cgtcttcttc accgtactgc tgtcctctgc ttacgctcac       60

ggtactccgc agaacatcac cgatattgtt ctgacccaga gtccggcaag cctggcagtt      120

agcctgggtc agcgtgcaac catgagctgt cgtgccggtg aaagcgttga tatttttggt      180

gttggttttc tgcattggta tcagcagaaa ccgggtcagc ctccgaaact gctgatttat      240

cgtgcaagca atctggaaag cggtattccg gttcgtttta gcggcaccgg tagccgtacc      300

gattttaccc tgattattga tccggttgaa gcagatgatg ttgccaccta ttattgtcag      360

cagaccaatg aagatccgta tacctttggt ggtggcacca aactggaaat caaaaccgtt      420

gcagcaccga gcgtttttat ctttccgcct agtgatgaac agctgaaaag cggtacagca      480

agcgttgttt gtctgctgaa taacttttat ccgcgtgaag caaaagttca gtggaaagtt      540

gataatgcac tgcagagcgg taatagccaa gaaagcgtta ccgaacagga tagcaaagat      600

agcacctata gcctgagcag taccctgacc ctgagcaaag cagattatga aaaacacaaa      660

gtgtatgcct gcgaagttac ccatcagggt ctgagcagtc cggttaccaa aagctttaat      720

cgtggtgaat gtctgaccaa caatggcacg ctgatgacgg gtatgagcgg tcaacaagcg      780

ggtaacgttc tggttgttaa gggcaattac catggcaata acggccagct ggtcatgaac      840

acggttctga acggcgatga tagcgtgacc gacaagctgg tggtcgaggg cgacacctct      900

ggtacgaccg cagtgacggt gaataatgca ggcggtacgg gtgccaaaac cctgaacggt      960

attgagttga tccacgttga cggtaagagc gagggcgagt ttgtgcaggc aggccgcatt     1020

gttgctggcg cttatgacta tacgctggcc cgtggtcagg gcgcgaatag cggtaactgg     1080

tatctgacca gcggctccga ctccccggaa ctgcaaccgg agcctgatcc gatgccgaat     1140

ccggagccaa acccgaaccc ggaaccgaac ccaaatccga ccccgactcc gggtccggac     1200

ttgaacgttg ataacgacct gcgtccggag gccggttcgt acatcgcgaa cctggcagcg     1260

gccaatacga tgtttacgac ccgtctgcac gaacgcctgg gtaataccta ctataccgat     1320

atggtcactg gtgaacagaa acaaaccacc atgtggatgc gccacgaggg tggtcacaat     1380

aagtggcgcg acggtagcgg ccagttgaaa acccagagca atcgctacgt tctgcaattg     1440

ggcggtgatg tggcgcaatg gagccaaaac ggcagcgacc gttggcatgt cggtgtgatg     1500

gcaggttacg gcaacagcga cagcaagacc atctccagcc gtaccggtta ccgtgcgaag     1560

gcaagcgtca acggttacag caccggcctg tatgccacct ggtatgctga tgatgagagc     1620

cgcaacggtg cttacttgga cagctgggca cagtattctt ggttcgataa tacggtgaaa     1680

ggcgacgacc tgcagagcga aagctacaaa tcgaaaggtt tcaccgcgag cctggaagcc     1740

ggctataagc acaaactggc ggaattcaat ggcagccagg gtactcgtaa cgaatggtac     1800

gttcaaccgc aggcgcaagt cacttggatg ggcgttaagg cggataaaca ccgtgagagc     1860

aacggtacgt tggtgcatag caacggtgat ggtaatgtcc aaacccgtct gggtgtgaaa     1920

acgtggctga agtcccatca caaaatggac gacggtaaat ctcgtgaatt tcagccgttc     1980

gtggaagtta actggctgca taatagcaag gatttcagca cgagcatgga tggtgtctcc     2040

gttacccagg acggcgcacg taacattgcg gagatcaaga ccggcgtcga gggtcagctg     2100

aatgcgaatc tgaatgtttg gggtaacgtg ggtgttcaag tagcggaccg tggttacaat     2160

gataccagcg cgatggtggg tattaagtgg cagttttaa                            2199


<210>  20
<211>  732
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of  fusion construct comprising a signal 
       peptide, the light chain and the EhaA autotransporter (linker 
       domain and autotransporter domain)


<220>
<221>  MISC_FEATURE
<223>  EhaA autotransporter: linker domain and transporter domain

<220>
<221>  MISC_FEATURE
<222>  (1)..(27)
<223>  signal peptide

<220>
<221>  MISC_FEATURE
<222>  (28)..(244)
<223>  light chain T84.66

<220>
<221>  MISC_FEATURE
<222>  (245)..(732)
<223>  EhaA autotransporter: linker domain and transporter domain

<400>  20

Met Ile Lys Leu Lys Phe Gly Val Phe Phe Thr Val Leu Leu Ser Ser 
1               5                   10                  15      


Ala Tyr Ala His Gly Thr Pro Gln Asn Ile Thr Asp Ile Val Leu Thr 
            20                  25                  30          


Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Met 
        35                  40                  45              


Ser Cys Arg Ala Gly Glu Ser Val Asp Ile Phe Gly Val Gly Phe Leu 
    50                  55                  60                  


His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
65                  70                  75                  80  


Arg Ala Ser Asn Leu Glu Ser Gly Ile Pro Val Arg Phe Ser Gly Thr 
                85                  90                  95      


Gly Ser Arg Thr Asp Phe Thr Leu Ile Ile Asp Pro Val Glu Ala Asp 
            100                 105                 110         


Asp Val Ala Thr Tyr Tyr Cys Gln Gln Thr Asn Glu Asp Pro Tyr Thr 
        115                 120                 125             


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Thr Val Ala Ala Pro Ser 
    130                 135                 140                 


Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala 
145                 150                 155                 160 


Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val 
                165                 170                 175     


Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser 
            180                 185                 190         


Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr 
        195                 200                 205             


Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys 
    210                 215                 220                 


Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn 
225                 230                 235                 240 


Arg Gly Glu Cys Leu Thr Asn Asn Gly Thr Leu Met Thr Gly Met Ser 
                245                 250                 255     


Gly Gln Gln Ala Gly Asn Val Leu Val Val Lys Gly Asn Tyr His Gly 
            260                 265                 270         


Asn Asn Gly Gln Leu Val Met Asn Thr Val Leu Asn Gly Asp Asp Ser 
        275                 280                 285             


Val Thr Asp Lys Leu Val Val Glu Gly Asp Thr Ser Gly Thr Thr Ala 
    290                 295                 300                 


Val Thr Val Asn Asn Ala Gly Gly Thr Gly Ala Lys Thr Leu Asn Gly 
305                 310                 315                 320 


Ile Glu Leu Ile His Val Asp Gly Lys Ser Glu Gly Glu Phe Val Gln 
                325                 330                 335     


Ala Gly Arg Ile Val Ala Gly Ala Tyr Asp Tyr Thr Leu Ala Arg Gly 
            340                 345                 350         


Gln Gly Ala Asn Ser Gly Asn Trp Tyr Leu Thr Ser Gly Ser Asp Ser 
        355                 360                 365             


Pro Glu Leu Gln Pro Glu Pro Asp Pro Met Pro Asn Pro Glu Pro Asn 
    370                 375                 380                 


Pro Asn Pro Glu Pro Asn Pro Asn Pro Thr Pro Thr Pro Gly Pro Asp 
385                 390                 395                 400 


Leu Asn Val Asp Asn Asp Leu Arg Pro Glu Ala Gly Ser Tyr Ile Ala 
                405                 410                 415     


Asn Leu Ala Ala Ala Asn Thr Met Phe Thr Thr Arg Leu His Glu Arg 
            420                 425                 430         


Leu Gly Asn Thr Tyr Tyr Thr Asp Met Val Thr Gly Glu Gln Lys Gln 
        435                 440                 445             


Thr Thr Met Trp Met Arg His Glu Gly Gly His Asn Lys Trp Arg Asp 
    450                 455                 460                 


Gly Ser Gly Gln Leu Lys Thr Gln Ser Asn Arg Tyr Val Leu Gln Leu 
465                 470                 475                 480 


Gly Gly Asp Val Ala Gln Trp Ser Gln Asn Gly Ser Asp Arg Trp His 
                485                 490                 495     


Val Gly Val Met Ala Gly Tyr Gly Asn Ser Asp Ser Lys Thr Ile Ser 
            500                 505                 510         


Ser Arg Thr Gly Tyr Arg Ala Lys Ala Ser Val Asn Gly Tyr Ser Thr 
        515                 520                 525             


Gly Leu Tyr Ala Thr Trp Tyr Ala Asp Asp Glu Ser Arg Asn Gly Ala 
    530                 535                 540                 


Tyr Leu Asp Ser Trp Ala Gln Tyr Ser Trp Phe Asp Asn Thr Val Lys 
545                 550                 555                 560 


Gly Asp Asp Leu Gln Ser Glu Ser Tyr Lys Ser Lys Gly Phe Thr Ala 
                565                 570                 575     


Ser Leu Glu Ala Gly Tyr Lys His Lys Leu Ala Glu Phe Asn Gly Ser 
            580                 585                 590         


Gln Gly Thr Arg Asn Glu Trp Tyr Val Gln Pro Gln Ala Gln Val Thr 
        595                 600                 605             


Trp Met Gly Val Lys Ala Asp Lys His Arg Glu Ser Asn Gly Thr Leu 
    610                 615                 620                 


Val His Ser Asn Gly Asp Gly Asn Val Gln Thr Arg Leu Gly Val Lys 
625                 630                 635                 640 


Thr Trp Leu Lys Ser His His Lys Met Asp Asp Gly Lys Ser Arg Glu 
                645                 650                 655     


Phe Gln Pro Phe Val Glu Val Asn Trp Leu His Asn Ser Lys Asp Phe 
            660                 665                 670         


Ser Thr Ser Met Asp Gly Val Ser Val Thr Gln Asp Gly Ala Arg Asn 
        675                 680                 685             


Ile Ala Glu Ile Lys Thr Gly Val Glu Gly Gln Leu Asn Ala Asn Leu 
    690                 695                 700                 


Asn Val Trp Gly Asn Val Gly Val Gln Val Ala Asp Arg Gly Tyr Asn 
705                 710                 715                 720 


Asp Thr Ser Ala Met Val Gly Ile Lys Trp Gln Phe 
                725                 730         


<210>  21
<211>  2910
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of  fusion construct comprising a signal peptide, 
       the heavy chain and the EhaA autotransporter (linker domain and 
       autotransporter domain)


<220>
<221>  misc_feature
<222>  (1)..(81)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (82)..(1443)
<223>  heavy chain T84.66

<220>
<221>  misc_feature
<222>  (1444)..(2910)
<223>  EhaA autotransporter: linker domain and transporter domain

<400>  21
atgatcaaac tgaaattcgg cgtcttcttc accgtactgc tgtcctctgc ttacgctcac       60

ggtactccgc agaacatcac cgaagttcag ctgcagcaga gcggtgcaga actggttgaa      120

ccgggtgcaa gcgttaaact gagctgtacc gcaagcggtt ttaacatcaa agatacctat      180

atgcattggg tgaaacagcg tccggaacag ggtctggaat ggattggtcg tattgatccg      240

gcaaatggca atagcaaata tgtgccgaaa tttcagggta aagcaaccat taccgcagat      300

accagcagca ataccgcata tctgcaactg accagcctga ccagtgaaga taccgcagtt      360

tattactgtg caccgtttgg ttattacgtt agcgattatg caatggcata ttggggtcag      420

ggcaccagcg ttaccgttag cagcgcaagc accaaaggtc cgagcgtttt tccgctggca      480

ccgagcagca aaagcaccag cggtggcacc gcagcactgg gttgtctggt taaagattat      540

tttccggaac cggttaccgt gagctggaat agcggtgcgc tgacctcagg tgttcatacc      600

tttccggcag tgctgcagag cagcggtctg tatagcctga gcagcgttgt gaccgttccg      660

agcagtagcc tgggcaccca gacctatatt tgtaatgtta atcataaacc gagcaacacc      720

aaagtggata aaaaagtgga accgaaaagc ccgaaaagtt gcgataaaac ccatacctgt      780

ccgccttgtc cggcaccgga actgctgggt ggtccgtcag tttttctgtt tccgcctaaa      840

ccgaaagata ccctgatgat tagccgtaca ccggaagtta cctgtgttgt tgttgatgtt      900

agccatgaag atccggaagt gaaatttaac tggtatgttg atggtgtgga agtgcataat      960

gcaaaaacca aaccgcgtga agaacagtat aattcaacct atcgtgttgt tagcgtgctg     1020

accgttctgc atcaggattg gctgaatggt aaagaataca aatgcaaagt gagcaacaaa     1080

gcactgcctg caccgattga aaaaaccatt agcaaagcaa aaggtcagcc tcgtgaaccg     1140

caggtttata ccctgcctcc gagccgtgat gaactgacca aaaatcaggt tagcctgacc     1200

tgtctggtga aaggttttta tccgagcgat attgcagttg aatgggaaag caatggtcag     1260

ccggaaaaca actataaaac cacccctccg gttctggata gtgatggtag ttttttcctg     1320

tatagcaaac tgaccgttga taaaagccgt tggcagcagg gtaatgtttt tagctgtagc     1380

gttatgcatg aagccctgca taatcattat acccagaaaa gcctgagcct gagtccgggt     1440

aaactgacca acaatggcac gctgatgacg ggtatgagcg gtcaacaagc gggtaacgtt     1500

ctggttgtta agggcaatta ccatggcaat aacggccagc tggtcatgaa cacggttctg     1560

aacggcgatg atagcgtgac cgacaagctg gtggtcgagg gcgacacctc tggtacgacc     1620

gcagtgacgg tgaataatgc aggcggtacg ggtgccaaaa ccctgaacgg tattgagttg     1680

atccacgttg acggtaagag cgagggcgag tttgtgcagg caggccgcat tgttgctggc     1740

gcttatgact atacgctggc ccgtggtcag ggcgcgaata gcggtaactg gtatctgacc     1800

agcggctccg actccccgga actgcaaccg gagcctgatc cgatgccgaa tccggagcca     1860

aacccgaacc cggaaccgaa cccaaatccg accccgactc cgggtccgga cttgaacgtt     1920

gataacgacc tgcgtccgga ggccggttcg tacatcgcga acctggcagc ggccaatacg     1980

atgtttacga cccgtctgca cgaacgcctg ggtaatacct actataccga tatggtcact     2040

ggtgaacaga aacaaaccac catgtggatg cgccacgagg gtggtcacaa taagtggcgc     2100

gacggtagcg gccagttgaa aacccagagc aatcgctacg ttctgcaatt gggcggtgat     2160

gtggcgcaat ggagccaaaa cggcagcgac cgttggcatg tcggtgtgat ggcaggttac     2220

ggcaacagcg acagcaagac catctccagc cgtaccggtt accgtgcgaa ggcaagcgtc     2280

aacggttaca gcaccggcct gtatgccacc tggtatgctg atgatgagag ccgcaacggt     2340

gcttacttgg acagctgggc acagtattct tggttcgata atacggtgaa aggcgacgac     2400

ctgcagagcg aaagctacaa atcgaaaggt ttcaccgcga gcctggaagc cggctataag     2460

cacaaactgg cggaattcaa tggcagccag ggtactcgta acgaatggta cgttcaaccg     2520

caggcgcaag tcacttggat gggcgttaag gcggataaac accgtgagag caacggtacg     2580

ttggtgcata gcaacggtga tggtaatgtc caaacccgtc tgggtgtgaa aacgtggctg     2640

aagtcccatc acaaaatgga cgacggtaaa tctcgtgaat ttcagccgtt cgtggaagtt     2700

aactggctgc ataatagcaa ggatttcagc acgagcatgg atggtgtctc cgttacccag     2760

gacggcgcac gtaacattgc ggagatcaag accggcgtcg agggtcagct gaatgcgaat     2820

ctgaatgttt ggggtaacgt gggtgttcaa gtagcggacc gtggttacaa tgataccagc     2880

gcgatggtgg gtattaagtg gcagttttaa                                      2910


<210>  22
<211>  969
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of  fusion construct comprising a signal 
       peptide, the heavy chain and the EhaA autotransporter (linker 
       domain and autotransporter domain)


<220>
<221>  MISC_FEATURE
<222>  (1)..(27)
<223>  signal peptide

<220>
<221>  MISC_FEATURE
<222>  (28)..(481)
<223>  heavy chain T84.66

<220>
<221>  MISC_FEATURE
<222>  (482)..(969)
<223>  EhaA autotransporter: linker domain and transporter domain

<400>  22

Met Ile Lys Leu Lys Phe Gly Val Phe Phe Thr Val Leu Leu Ser Ser 
1               5                   10                  15      


Ala Tyr Ala His Gly Thr Pro Gln Asn Ile Thr Glu Val Gln Leu Gln 
            20                  25                  30          


Gln Ser Gly Ala Glu Leu Val Glu Pro Gly Ala Ser Val Lys Leu Ser 
        35                  40                  45              


Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr Met His Trp Val 
    50                  55                  60                  


Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Arg Ile Asp Pro 
65                  70                  75                  80  


Ala Asn Gly Asn Ser Lys Tyr Val Pro Lys Phe Gln Gly Lys Ala Thr 
                85                  90                  95      


Ile Thr Ala Asp Thr Ser Ser Asn Thr Ala Tyr Leu Gln Leu Thr Ser 
            100                 105                 110         


Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Pro Phe Gly Tyr 
        115                 120                 125             


Tyr Val Ser Asp Tyr Ala Met Ala Tyr Trp Gly Gln Gly Thr Ser Val 
    130                 135                 140                 


Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
145                 150                 155                 160 


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
                165                 170                 175     


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
            180                 185                 190         


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
        195                 200                 205             


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
    210                 215                 220                 


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
225                 230                 235                 240 


Lys Val Asp Lys Lys Val Glu Pro Lys Ser Pro Lys Ser Cys Asp Lys 
                245                 250                 255     


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
            260                 265                 270         


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
        275                 280                 285             


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
    290                 295                 300                 


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
305                 310                 315                 320 


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
                325                 330                 335     


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
            340                 345                 350         


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
        355                 360                 365             


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
    370                 375                 380                 


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
385                 390                 395                 400 


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
                405                 410                 415     


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
            420                 425                 430         


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
        435                 440                 445             


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
    450                 455                 460                 


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
465                 470                 475                 480 


Lys Leu Thr Asn Asn Gly Thr Leu Met Thr Gly Met Ser Gly Gln Gln 
                485                 490                 495     


Ala Gly Asn Val Leu Val Val Lys Gly Asn Tyr His Gly Asn Asn Gly 
            500                 505                 510         


Gln Leu Val Met Asn Thr Val Leu Asn Gly Asp Asp Ser Val Thr Asp 
        515                 520                 525             


Lys Leu Val Val Glu Gly Asp Thr Ser Gly Thr Thr Ala Val Thr Val 
    530                 535                 540                 


Asn Asn Ala Gly Gly Thr Gly Ala Lys Thr Leu Asn Gly Ile Glu Leu 
545                 550                 555                 560 


Ile His Val Asp Gly Lys Ser Glu Gly Glu Phe Val Gln Ala Gly Arg 
                565                 570                 575     


Ile Val Ala Gly Ala Tyr Asp Tyr Thr Leu Ala Arg Gly Gln Gly Ala 
            580                 585                 590         


Asn Ser Gly Asn Trp Tyr Leu Thr Ser Gly Ser Asp Ser Pro Glu Leu 
        595                 600                 605             


Gln Pro Glu Pro Asp Pro Met Pro Asn Pro Glu Pro Asn Pro Asn Pro 
    610                 615                 620                 


Glu Pro Asn Pro Asn Pro Thr Pro Thr Pro Gly Pro Asp Leu Asn Val 
625                 630                 635                 640 


Asp Asn Asp Leu Arg Pro Glu Ala Gly Ser Tyr Ile Ala Asn Leu Ala 
                645                 650                 655     


Ala Ala Asn Thr Met Phe Thr Thr Arg Leu His Glu Arg Leu Gly Asn 
            660                 665                 670         


Thr Tyr Tyr Thr Asp Met Val Thr Gly Glu Gln Lys Gln Thr Thr Met 
        675                 680                 685             


Trp Met Arg His Glu Gly Gly His Asn Lys Trp Arg Asp Gly Ser Gly 
    690                 695                 700                 


Gln Leu Lys Thr Gln Ser Asn Arg Tyr Val Leu Gln Leu Gly Gly Asp 
705                 710                 715                 720 


Val Ala Gln Trp Ser Gln Asn Gly Ser Asp Arg Trp His Val Gly Val 
                725                 730                 735     


Met Ala Gly Tyr Gly Asn Ser Asp Ser Lys Thr Ile Ser Ser Arg Thr 
            740                 745                 750         


Gly Tyr Arg Ala Lys Ala Ser Val Asn Gly Tyr Ser Thr Gly Leu Tyr 
        755                 760                 765             


Ala Thr Trp Tyr Ala Asp Asp Glu Ser Arg Asn Gly Ala Tyr Leu Asp 
    770                 775                 780                 


Ser Trp Ala Gln Tyr Ser Trp Phe Asp Asn Thr Val Lys Gly Asp Asp 
785                 790                 795                 800 


Leu Gln Ser Glu Ser Tyr Lys Ser Lys Gly Phe Thr Ala Ser Leu Glu 
                805                 810                 815     


Ala Gly Tyr Lys His Lys Leu Ala Glu Phe Asn Gly Ser Gln Gly Thr 
            820                 825                 830         


Arg Asn Glu Trp Tyr Val Gln Pro Gln Ala Gln Val Thr Trp Met Gly 
        835                 840                 845             


Val Lys Ala Asp Lys His Arg Glu Ser Asn Gly Thr Leu Val His Ser 
    850                 855                 860                 


Asn Gly Asp Gly Asn Val Gln Thr Arg Leu Gly Val Lys Thr Trp Leu 
865                 870                 875                 880 


Lys Ser His His Lys Met Asp Asp Gly Lys Ser Arg Glu Phe Gln Pro 
                885                 890                 895     


Phe Val Glu Val Asn Trp Leu His Asn Ser Lys Asp Phe Ser Thr Ser 
            900                 905                 910         


Met Asp Gly Val Ser Val Thr Gln Asp Gly Ala Arg Asn Ile Ala Glu 
        915                 920                 925             


Ile Lys Thr Gly Val Glu Gly Gln Leu Asn Ala Asn Leu Asn Val Trp 
    930                 935                 940                 


Gly Asn Val Gly Val Gln Val Ala Asp Arg Gly Tyr Asn Asp Thr Ser 
945                 950                 955                 960 


Ala Met Val Gly Ile Lys Trp Gln Phe 
                965                 


