                         SEQUENCE LISTING

<110>  JBS Science Inc.
       SONG, Wei
       JAIN, Surbhi
       STEFFEN, Jamin D
       WANG, Jeremy
 
<120>  KIT AND METHOD FOR DETECTING MUTATIONS IN CTNNB1 AND HTERT, AND 
       USE THEREOF IN HCC DETECTION AND DISEASE MANAGEMENT

<130>  JBS0012-1

<140>  PCT/US2017/28668
<141>  2017-04-20

<150>  US 62/325,457
<151>  2016-04-20

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  DNA
<213>  Homo sapiens

<400>  1
gcagcaacag tcttacct                                                     18


<210>  2
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  non-complementary accessory tag

<220>
<221>  misc_feature
<222>  (25)..(40)
<223>  CTNNB1 sequence

<400>  2
ctgtgtgctc ttcgtgtgtg gtgtctgtgg tagtggcacc                             40


<210>  3
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BNA clamp


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  phosphorylation modification

<400>  3
ggactctgga atccattc                                                     18


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hydrolysis probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  fluorescent tag 6FAM

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  fluorescent tag BHQ1

<400>  4
ggactctgga atccattctg gtgcca                                            26


<210>  5
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  5
gagtgaagga ctgagaaaa                                                    19


<210>  6
<211>  18
<212>  DNA
<213>  Homo sapiens

<400>  6
ctgatttgat ggagttgg                                                     18


<210>  7
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  non-complementary accessory tag

<220>
<221>  misc_feature
<222>  (34)..(51)
<223>  CTNNB1 sequence

<400>  7
tcgtcggcag cgtcagatgt gtataagaga caggcagcaa cagtcttacc t                51


<210>  8
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized


<220>
<221>  misc_feature
<222>  (1)..(34)
<223>  non-complementary accessory tag

<220>
<221>  misc_feature
<222>  (35)..(58)
<223>  CTNNB1 sequence

<400>  8
gtctcgtggg ctcggagatg tgtataagag acagctgtgt gctcttcgtg tgtggtgt         58


<210>  9
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  9
tcgtcggcag cgtc                                                         14


<210>  10
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized

<400>  10
gtctcgtggg ctcgga                                                       16


<210>  11
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  non-complementary accessory tag

<220>
<221>  misc_feature
<222>  (34)..(51)
<223>  hTERT sequence

<400>  11
tcgtcggcag cgtcagatgt gtataagaga cagaggggct gggagggccc g                51


<210>  12
<211>  18
<212>  DNA
<213>  Homo sapiens

<400>  12
gacccctccc gggtcccc                                                     18


<210>  13
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BNA clamp


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  bridged nucleic acid

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  phosphorylation modification

<400>  13
gccccctccg g                                                            11


<210>  14
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  14
ccgacccctc ccggg                                                        15


<210>  15
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hydrolysis probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  fluorescent tag 6FAM

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  fluorescent tag BHQ1

<400>  15
cggagggggc tgggccgg                                                     18


<210>  16
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hydrolysis probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  fluorescent tag 6FAM

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  fluorescent tag BHQ1

<400>  16
cggaaggggc tgggccgg                                                     18


