
                                SEQUENCE LISTING

<110> President and Fellows of Harvard College

<120> METHODS FOR INCREASING EFFICIENCY OF
  NUCLEASE-MEDIATED GENE EDITING IN INDUCED PLURIPOTENT STEM
  CELLS

<130> 010498.00772

<160> 32

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> THY1 coding exon sgRNA

<400> 1
gggtcaggct gaactcgtac tgg                                              23

<210> 2
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> THY1 truncated sgRNA

<400> 2
gtcaggctga actcgtactg g                                                21

<210> 3
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> POU5F1 coding exon sgRNA

<400> 3
gctggagcaa aacccggagg agg                                              23

<210> 4
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> negative sgRNA #1

<400> 4
gcttcaaaga acggcgctaa c                                                21

<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> negative sgRNA #2

<400> 5
ggagacgatt aatgcgtctc g                                                21

<210> 6
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD147 coding exon sgRNA

<400> 6
gtcgtcagaa cacatcaacg agg                                              23

<210> 7
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD147 truncated sgRNA

<400> 7
gcgtcagaac acatcaacga gg                                               22

<210> 8
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> THY1 left sgRNA

<400> 8
gcacagtctc agaaaagcgc agg                                              23

<210> 9
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> THY1 right sgRNA

<400> 9
gttagtagca acgctacccc agg                                              23

<210> 10
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD147 left sgRNA

<400> 10
gatttcctgc gctgaatcgg gtgg                                             24

<210> 11
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD147 right sgRNA

<400> 11
gtggctcctg tctgtgcctg acgg                                             24

<210> 12
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Jak2 sgRNA

<400> 12
gaattatgga gtatgtgtct gtgg                                             24

<210> 13
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> HLA-A sgRNA

<400> 13
ggacctgcgc tcttggaccg cgg                                              23

<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> HLA-A trunctated sgRNA

<400> 14
gacctgcgct cttggaccgc gg                                               22

<210> 15
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> HLA-B or C sgRNA

<400> 15
gggccgcctc ccacttgcgc tgg                                              23

<210> 16
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> HLA-B or C truncated sgRNA

<400> 16
gccgcctccc acttgcgctg g                                                21

<210> 17
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> Y28A primer

<400> 17
gtcgcagagg ggcgccgagt gggatgcg                                         28

<210> 18
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> Y28A primer

<400> 18
cgcatcccac tcggcgcccc tctgcgac                                         28

<210> 19
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> G145A primer

<400> 19
aggccacaat cctcgcccag ttcaccccg                                        29

<210> 20
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> G145A primer

<400> 20
cggggtgaac tgggcgagga ttgtggcct                                        29

<210> 21
<211> 109
<212> DNA
<213> Artificial Sequence

<220> 
<223> ssODN encoding Jak2 V617 mutation (plus)

<400> 21
aagtatgatg agcaagcttt ctcacaagca tttggtttta aattatggag tatgttctgt      60
ggagacgaga gtaagtaaaa ctacaggctt tctaatgcct ttctcagag                 109

<210> 22
<211> 110
<212> DNA
<213> Artificial Sequence

<220> 
<223> ssODN encoding Jak2 V617 mutation (minus)

<400> 22
ctctgagaaa ggcattagaa agcctgtagt tttacttact ctcgtctcca cagaaacata      60
ctccataatt taaaaccaaa tgcttgtgag aaagcttgct catcatactt                110

<210> 23
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 23
gtgcaggctt tcaacaatta ctttg                                            25

<210> 24
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 24
tctcgtctcc acagaaacat actccataat ttaaaacc                              38

<210> 25
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 25
attatggagt atgtttctgt ggagacgaga gtaagtaaaa ctacag                     46

<210> 26
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 26
atcttgctct agattgggct ttgg                                             24

<210> 27
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 27
cttgagccaa agcccaatct agagcaagat tagacgtcag gtggcacttt tc              52

<210> 28
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 28
ctttacaaag taattgttga aagcctgcac gtttgcgtat tgggcgctct tc              52

<210> 29
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 29
ctttccctac acgacgctct tccgatcttt atggacaaca gtcaaacaac aatt            54

<210> 30
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 30
ggagttcaga cgtgtgctct tccgatctac acctagctgt gatcctgaaa ctg             53

<210> 31
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> peptide V5

<400> 31
Val Pro Met Leu Lys
 1               5

<210> 32
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> control peptide

<400> 32
Ile Pro Met Ile Lys
 1               5