                         SEQUENCE LISTING

<110>  Universitaet Basel
 
<120>  A method for evaluating a candidate compound (T) for treating 
       Myotonic Dystrophy Type I (DM1)

<130>  uz233wo

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  253
<212>  PRT
<213>  Homo sapiens (MBNL1)

<400>  1

Met Ala Val Ser Val Thr Pro Ile Arg Asp Thr Lys Trp Leu Thr Leu 
1               5                   10                  15      


Glu Val Cys Arg Glu Phe Gln Arg Gly Thr Cys Ser Arg Pro Asp Thr 
            20                  25                  30          


Glu Cys Lys Phe Ala His Pro Ser Lys Ser Cys Gln Val Glu Asn Gly 
        35                  40                  45              


Arg Val Ile Ala Cys Phe Asp Ser Leu Lys Gly Arg Cys Ser Arg Glu 
    50                  55                  60                  


Asn Cys Lys Tyr Leu His Pro Pro Pro His Leu Lys Thr Gln Leu Glu 
65                  70                  75                  80  


Ile Asn Gly Arg Asn Asn Leu Ile Gln Gln Lys Asn Met Ala Met Leu 
                85                  90                  95      


Ala Gln Gln Met Gln Leu Ala Asn Ala Met Met Pro Gly Ala Pro Leu 
            100                 105                 110         


Gln Pro Val Pro Met Phe Ser Val Ala Pro Ser Leu Ala Thr Asn Ala 
        115                 120                 125             


Ser Ala Ala Ala Phe Asn Pro Tyr Leu Gly Pro Val Ser Pro Ser Leu 
    130                 135                 140                 


Val Pro Ala Glu Ile Leu Pro Thr Ala Pro Met Leu Val Thr Gly Asn 
145                 150                 155                 160 


Pro Gly Val Pro Val Pro Ala Ala Ala Ala Ala Ala Ala Gln Lys Leu 
                165                 170                 175     


Met Arg Thr Asp Arg Leu Glu Val Cys Arg Glu Tyr Gln Arg Gly Asn 
            180                 185                 190         


Cys Asn Arg Gly Glu Asn Asp Cys Arg Phe Ala His Pro Ala Asp Ser 
        195                 200                 205             


Thr Met Ile Asp Thr Asn Asp Asn Thr Val Thr Val Cys Met Asp Tyr 
    210                 215                 220                 


Ile Lys Gly Arg Cys Ser Arg Glu Lys Cys Lys Tyr Phe His Pro Pro 
225                 230                 235                 240 


Ala His Leu Gln Ala Lys Ile Lys Ala Ala Gln Tyr Gln 
                245                 250             


<210>  2
<211>  234
<212>  DNA
<213>  Homo sapiens

<400>  2
ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg       60

ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg      120

ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg      180

ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctg            234


<210>  3
<211>  234
<212>  RNA
<213>  Homo sapiens

<400>  3
cugcugcugc ugcugcugcu gcugcugcug cugcugcugc ugcugcugcu gcugcugcug       60

cugcugcugc ugcugcugcu gcugcugcug cugcugcugc ugcugcugcu gcugcugcug      120

cugcugcugc ugcugcugcu gcugcugcug cugcugcugc ugcugcugcu gcugcugcug      180

cugcugcugc ugcugcugcu gcugcugcug cugcugcugc ugcugcugcu gcug            234


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for the DMPK gene from homo sapiens

<400>  4
cagctccagt cctgtgatcc                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for the DMPK gene from homo sapiens

<400>  5
ctggccgaaa gaaagaaatg                                                   20


<210>  6
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 CUG forward primer

<400>  6
taatacgact cactataggc agctccagtc ctgtgatcc                              39


<210>  7
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 CUG reverse primer

<400>  7
taatacgact cactataggc tggccgaaag aaagaaatg                              39


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (RT-PCR) for INSR from homo sapiens

<400>  8
ccaaagacag actctcagat                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (RT-PCR) for INSR from homo sapiens

<400>  9
aacatcgcca agggacctgc                                                   20


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (RT-PCR) for TNNT2 from homo sapiens

<400>  10
atagaagagg tggtggaaga gtac                                              24


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (RT-PCR) for TNNT2 from homo sapiens

<400>  11
gtctcagcct ctgcttcagc atcc                                              24


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (RT-qPCR) for INSR from homo sapiens

<400>  12
gacctggtct ccaccattcg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (exon) (RT-qPCR) for INSR from homo sapiens

<400>  13
caccagtgcc tgaagaggtt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (pan) (RT-qPCR) for INSR from homo sapiens

<400>  14
acgaaaacca cgttgtgcag                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (RT-qPCR) for TNNT2 from homo sapiens

<400>  15
ctgctgttct gagggagagc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (exon) (RT-qPCR) for TNNT2 from homo sapiens

<400>  16
tcgtcctctc tccagtcctc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (pan) (RT-qPCR) for TNNT2 from homo sapiens

<400>  17
ctgctcctcc tcctcgtact                                                   20


<210>  18
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (RT-qPCR) for beta-actin from homo sapiens

<400>  18
ccaaccgcga gaagatga                                                     18


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (RT-qPCR) for beta-actin from homo sapiens

<400>  19
ccagaggcgt acagggatag                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (RT-qPCR) for ATE1 from homo sapiens

<400>  20
gggtttccag gctcaaggtc                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (exon) (RT-qPCR) for ATE1 from homo sapiens

<400>  21
tgaactgcga acttggtgga                                                   20


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (pan) (RT-qPCR) for ATE1 from homo sapiens

<400>  22
tgtgtgatgc attctctggt aa                                                22


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (RT-qPCR) for FHL1 from homo sapiens

<400>  23
atgccgattg ctttgtgtgt                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (exon) (RT-qPCR) for FHL1 from homo sapiens

<400>  24
ctgggtggct cactcttgac                                                   20


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (pan) RT-qPCR) for FHL1 from homo sapiens

<400>  25
tcttgcatcc agcacacttc t                                                 21


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forwar primer (RT-PCR) for CLCN1 from mus musculus

<400>  26
ggaatacctc acactcaagg cc                                                22


<210>  27
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (RT-PCR) for CLCN1 from mus musculus

<400>  27
cacggaacac aaaggcactg aatgt                                             25


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward rpimer (RT-PCR) for SERCA1 from mus musculus

<400>  28
gctcatggtc ctcaagatct cac                                               23


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (RT-PCR) for SERCA1 from mus musculus

<400>  29
gggtcagtgc ctcagctttg                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (exon) (RT-qPCR) for CLCN1 from mus musculus

<400>  30
gggcgtggga tgctactttg                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (pan) (RT-qPCR) for CLCN1 from mus musculus

<400>  31
ctgacatcct gacagtgggc                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (RT-qPCR) for CLCN1 from mus musculus

<400>  32
aggacacgga acacaaaggc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (RT-qPCR) for SERCA1 from mus musculus

<400>  33
gccctggact ttacccagtg                                                   20


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (exon) (RT-qPCR) for SERCA1 from mus musculus

<400>  34
acggttcaaa gacatggagg a                                                 21


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (pan) (RT-qPCR) for SERCA1 from mus musculus

<400>  35
cctccagata gttccgagca                                                   20


<210>  36
<211>  9
<212>  RNA
<213>  Homo sapiens

<400>  36
cugcugcug                                                                9


<210>  37
<211>  388
<212>  PRT
<213>  Homo sapiens (MBNL1, Q9NR56)

<400>  37

Met Ala Val Ser Val Thr Pro Ile Arg Asp Thr Lys Trp Leu Thr Leu 
1               5                   10                  15      


Glu Val Cys Arg Glu Phe Gln Arg Gly Thr Cys Ser Arg Pro Asp Thr 
            20                  25                  30          


Glu Cys Lys Phe Ala His Pro Ser Lys Ser Cys Gln Val Glu Asn Gly 
        35                  40                  45              


Arg Val Ile Ala Cys Phe Asp Ser Leu Lys Gly Arg Cys Ser Arg Glu 
    50                  55                  60                  


Asn Cys Lys Tyr Leu His Pro Pro Pro His Leu Lys Thr Gln Leu Glu 
65                  70                  75                  80  


Ile Asn Gly Arg Asn Asn Leu Ile Gln Gln Lys Asn Met Ala Met Leu 
                85                  90                  95      


Ala Gln Gln Met Gln Leu Ala Asn Ala Met Met Pro Gly Ala Pro Leu 
            100                 105                 110         


Gln Pro Val Pro Met Phe Ser Val Ala Pro Ser Leu Ala Thr Asn Ala 
        115                 120                 125             


Ser Ala Ala Ala Phe Asn Pro Tyr Leu Gly Pro Val Ser Pro Ser Leu 
    130                 135                 140                 


Val Pro Ala Glu Ile Leu Pro Thr Ala Pro Met Leu Val Thr Gly Asn 
145                 150                 155                 160 


Pro Gly Val Pro Val Pro Ala Ala Ala Ala Ala Ala Ala Gln Lys Leu 
                165                 170                 175     


Met Arg Thr Asp Arg Leu Glu Val Cys Arg Glu Tyr Gln Arg Gly Asn 
            180                 185                 190         


Cys Asn Arg Gly Glu Asn Asp Cys Arg Phe Ala His Pro Ala Asp Ser 
        195                 200                 205             


Thr Met Ile Asp Thr Asn Asp Asn Thr Val Thr Val Cys Met Asp Tyr 
    210                 215                 220                 


Ile Lys Gly Arg Cys Ser Arg Glu Lys Cys Lys Tyr Phe His Pro Pro 
225                 230                 235                 240 


Ala His Leu Gln Ala Lys Ile Lys Ala Ala Gln Tyr Gln Val Asn Gln 
                245                 250                 255     


Ala Ala Ala Ala Gln Ala Ala Ala Thr Ala Ala Ala Met Thr Gln Ser 
            260                 265                 270         


Ala Val Lys Ser Leu Lys Arg Pro Leu Glu Ala Thr Phe Asp Leu Gly 
        275                 280                 285             


Ile Pro Gln Ala Val Leu Pro Pro Leu Pro Lys Arg Pro Ala Leu Glu 
    290                 295                 300                 


Lys Thr Asn Gly Ala Thr Ala Val Phe Asn Thr Gly Ile Phe Gln Tyr 
305                 310                 315                 320 


Gln Gln Ala Leu Ala Asn Met Gln Leu Gln Gln His Thr Ala Phe Leu 
                325                 330                 335     


Pro Pro Val Pro Met Val His Gly Ala Thr Pro Ala Thr Val Ser Ala 
            340                 345                 350         


Ala Thr Thr Ser Ala Thr Ser Val Pro Phe Ala Ala Thr Ala Thr Ala 
        355                 360                 365             


Asn Gln Ile Pro Ile Ile Ser Ala Glu His Leu Thr Ser His Lys Tyr 
    370                 375                 380                 


Val Thr Gln Met 
385             


<210>  38
<211>  9
<212>  DNA
<213>  Homo sapiens

<400>  38
ctgctgctg                                                                9


