                         SEQUENCE LISTING

<110>  ACADEMIA SINICA
 
<120>  INHIBITION OF SCUBE2, A NOVEL VEGFR2 CO-RECEPTOR, SUPPRESSES 
       TUMOR ANGIOGENESIS

<130>  10011-065PCT

<150>  62322626
<151>  2016-04-14

<160>  102   

<170>  PatentIn version 3.5

<210>  1
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VH  domain

<400>  1

Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Arg Lys Leu Ser Cys Val Ala Ser Gly Phe Thr Ile Ser Ser Phe 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Glu Arg Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Ser Asn Thr Ile Tyr Tyr Ala Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Pro Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Thr Ser Leu Trp Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Thr Leu Asp Gly Trp Phe Thr Tyr Trp Gly Arg Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Thr 
        115                 


<210>  2
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VL domain

<400>  2

Asp Ile Val Met Thr Gln Pro Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Leu His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Leu Phe Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Asn Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Ala Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Leu Gln Gly 
                85                  90                  95      


Ser His Val Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VH  CDR1

<400>  3

Gly Phe Thr Ile Ser Ser Phe Gly Met His 
1               5                   10  


<210>  4
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VH  CDR2

<400>  4

Tyr Ile Ser Ser Gly Ser Asn Thr Ile Tyr Tyr Ala Asp Thr Val Lys 
1               5                   10                  15      


Gly 
    


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VH CDR3

<400>  5

Ser Gly Thr Leu Asp Gly Trp Phe Thr Tyr 
1               5                   10  


<210>  6
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VL  CDR1

<400>  6

Arg Ser Ser Gln Ser Ile Leu His Ser Asn Gly Asn Thr Tyr Leu Glu 
1               5                   10                  15      


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VL CDR2

<400>  7

Lys Leu Phe Asn Arg Phe Ser 
1               5           


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VL CDR3

<400>  8

Leu Gln Gly Ser His Val Pro Pro Thr 
1               5                   


<210>  9
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VH FW1

<400>  9

Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Arg Lys Leu Ser Cys Val Ala Ser 
            20                  25  


<210>  10
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VH FW2

<400>  10

Trp Val Arg Gln Ala Pro Glu Arg Gly Leu Glu Trp Val Ala 
1               5                   10                  


<210>  11
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VH FW3

<400>  11

Arg Phe Thr Ile Ser Arg Asp Asn Pro Lys Asn Thr Leu Phe Leu Gln 
1               5                   10                  15      


Met Thr Ser Leu Trp Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Arg 
            20                  25                  30          


<210>  12
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VH  FW4

<400>  12

Trp Gly Arg Gly Thr Leu Val Thr Val Ser Thr 
1               5                   10      


<210>  13
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VL  FW1

<400>  13

Asp Ile Val Met Thr Gln Pro Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys 
            20              


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VL FW2

<400>  14

Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  15
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VL  FW3

<400>  15

Gly Val Pro Asp Arg Phe Asn Gly Ser Gly Ser Gly Thr Asp Phe Thr 
1               5                   10                  15      


Leu Lys Ile Ser Arg Ala Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys 
            20                  25                  30          


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb EGF-C3 VL FW4

<400>  16

Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 
1               5                   10  


<210>  17
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1 /B1 VH  domain

<400>  17

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met Tyr Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Asn Asn Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met His Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Tyr Arg Tyr Leu Ala Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala 
        115                 


<210>  18
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1 /B1 VL  domain

<400>  18

Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Thr Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Arg Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Ile Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VH CDR1

<400>  19

Gly Tyr Ala Phe Thr Ser Tyr Asn Met Tyr 
1               5                   10  


<210>  20
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VH CDR2

<400>  20

Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Asn Asn Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VH  CDR3

<400>  21

Ser Tyr Arg Tyr Leu Ala Trp Phe Ala Tyr 
1               5                   10  


<210>  22
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1VL  CDR1

<400>  22

Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu 
1               5                   10                  15      


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VL  CDR2

<400>  23

Thr Val Ser Asn Arg Phe Ser 
1               5           


<210>  24
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VL  CDR3

<400>  24

Phe Gln Gly Ser His Ile Pro Tyr Thr 
1               5                   


<210>  25
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VH  FW1

<400>  25

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser 
            20                  25  


<210>  26
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VH  FW2

<400>  26

Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile Gly 
1               5                   10                  


<210>  27
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VH  FW3

<400>  27

Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met His 
1               5                   10                  15      


Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg 
            20                  25                  30          


<210>  28
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VH  FW4

<400>  28

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
1               5                   10      


<210>  29
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VL  FW1

<400>  29

Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys 
            20              


<210>  30
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VL  FW2

<400>  30

Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  31
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VL  FW3

<400>  31

Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
1               5                   10                  15      


Leu Arg Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys 
            20                  25                  30          


<210>  32
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-A1/B1 VL  FW4

<400>  32

Phe Gly Gly Gly Thr Lys Leu Glu Leu Lys 
1               5                   10  


<210>  33
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VH  domain

<400>  33

Glu Val Gln Arg Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Arg Asn Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Thr Thr Glu Lys Lys Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Asn Asp Val Gly Ser Tyr Thr Tyr Phe Pro Ala Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Thr Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Val Thr Ser Ser Asp Thr Val Pro His His His Ala Leu Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Ser Ser Val Thr Val Ser Ser 
        115                 120         


<210>  34
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VL  domain

<400>  34

Asp Ile Val Met Thr Gln Pro Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Val Phe Cys Arg Ala Ser Gln Asn Val Asp Ser Arg 
            20                  25                  30          


Gly Ile Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Arg Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Ser Val 
                85                  90                  95      


Glu Asp Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110 


<210>  35
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VH  CDR1

<400>  35

Gly Phe Thr Phe Arg Asn Tyr Ala Met Ser 
1               5                   10  


<210>  36
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VH  CDR2

<400>  36

Thr Ile Asn Asp Val Gly Ser Tyr Thr Tyr Phe Pro Ala Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  37
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VH  CDR3

<400>  37

Ser Ser Asp Thr Val Pro His His His Ala Leu Asp Tyr 
1               5                   10              


<210>  38
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VL CDR1

<400>  38

Arg Ala Ser Gln Asn Val Asp Ser Arg Gly Ile Ser Phe Met His 
1               5                   10                  15  


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VL CDR2

<400>  39

Ala Ala Ser Asn Leu Glu Ser 
1               5           


<210>  40
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VL CDR3

<400>  40

Gln Gln Ser Val Glu Asp Leu Thr 
1               5               


<210>  41
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VH  FW1

<400>  41

Glu Val Gln Arg Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser 
            20                  25  


<210>  42
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VH  FW2

<400>  42

Trp Val Arg Gln Thr Thr Glu Lys Lys Leu Glu Trp Val Ala 
1               5                   10                  


<210>  43
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VH  FW3

<400>  43

Arg Phe Thr Val Ser Arg Asp Asn Thr Lys Asn Thr Leu Tyr Leu Gln 
1               5                   10                  15      


Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Val Thr 
            20                  25                  30          


<210>  44
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VH  FW4

<400>  44

Trp Gly Gln Gly Ser Ser Val Thr Val Ser Ser 
1               5                   10      


<210>  45
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VL FW1

<400>  45

Asp Ile Val Met Thr Gln Pro Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Val Phe Cys 
            20              


<210>  46
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VL FW2

<400>  46

Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  47
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VL  FW3

<400>  47

Gly Ile Pro Ala Arg Phe Arg Gly Ser Gly Ser Gly Thr Asp Phe Thr 
1               5                   10                  15      


Leu Asn Ile His Pro Val Glu Glu Glu Asp Val Ala Thr Tyr Tyr Cys 
            20                  25                  30          


<210>  48
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb SP-B2 VL FW4

<400>  48

Phe Gly Gly Gly Thr Lys Leu Glu Leu Lys 
1               5                   10  


<210>  49
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VH  domain

<400>  49

Glu Val Gln Arg Val Glu Ser Gly Gly Asp Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Thr Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Arg Asn Tyr 
            20                  25                  30          


Gly Met Ser Trp Val Arg Gln Thr Pro Asp Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Ser Gly Gly Val Tyr Ile Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Ser Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Asp Tyr Asp Gly Arg Gly Tyr Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Thr Leu Thr Val Ser Ser 
        115                 120 


<210>  50
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VL  domain

<400>  50

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Arg Thr Arg Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Lys Gln 
                85                  90                  95      


Ser Tyr Asn Leu Pro Thr Phe Gly Ser Gly Thr Lys Leu Asp Ile Lys 
            100                 105                 110         


<210>  51
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VH CDR1

<400>  51

Gly Phe Thr Phe Arg Asn Tyr Gly Met Ser 
1               5                   10  


<210>  52
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VH  CDR2

<400>  52

Thr Ile Ser Ser Gly Gly Val Tyr Ile Tyr Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  53
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VH  CDR3

<400>  53

Ala Arg Asp Asp Tyr Asp Gly Arg Gly Tyr Phe Asp Tyr 
1               5                   10              


<210>  54
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VL  CDR1

<400>  54

Lys Ser Ser Gln Ser Leu Leu Asn Ser Arg Thr Arg Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  55
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VL  CDR2

<400>  55

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  56
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VL  CDR3

<400>  56

Lys Gln Ser Tyr Asn Leu Pro Thr 
1               5               


<210>  57
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VH  FW1

<400>  57

Glu Val Gln Arg Val Glu Ser Gly Gly Asp Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Thr Leu Lys Leu Ser Cys Ala Ala Ser 
            20                  25  


<210>  58
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VH  FW2

<400>  58

Trp Val Arg Gln Thr Pro Asp Lys Arg Leu Glu Trp Val Ala 
1               5                   10                  


<210>  59
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VH  FW3

<400>  59

Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Phe Leu Gln 
1               5                   10                  15      


Met Ser Ser Leu Arg Ser Glu Asp Ser Ala Met Tyr Tyr Cys 
            20                  25                  30  


<210>  60
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VH  FW4

<400>  60

Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 
1               5                   10      


<210>  61
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VL  FW1

<400>  61

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Ala Val Ser Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys 
            20              


<210>  62
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VL  FW2

<400>  62

Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  63
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VL  FW3

<400>  63

Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr 
1               5                   10                  15      


Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys 
            20                  25                  30          


<210>  64
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb CR-#5 VL  FW4

<400>  64

Phe Gly Ser Gly Thr Lys Leu Asp Ile Lys 
1               5                   10  


<210>  65
<211>  3086
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE2 cDNA

<400>  65
atgggggtcg cgggccgcaa ccgtcccggg gcggcctggg cggtgctgct gctgctgctg       60

ctgctgccgc cactgctgct gctggcgggg gccgtcccgc cgggtcgggg ccgtgccgcg      120

gggccgcagg aggatgtaga tgagtgtgcc caagggctag atgactgcca tgccgacgcc      180

ctgtgtcaga acacacccac ctcctacaag tgctcctgca agcctggcta ccaaggggaa      240

ggcaggcagt gtgaggacat cgatgaatgt ggaaatgagc tcaatggagg ctgtgtccat      300

gactgtttga atattccagg caattatcgt tgcacttgtt ttgatggctt catgttggct      360

catgacggtc ataattgtct tgatgtggac gagtgcctgg agaacaatgg cggctgccag      420

catacctgtg tcaacgtcat ggggagctat gagtgctgct gcaaggaggg gtttttcctg      480

agtgacaatc agcacacctg cattcaccgc tcggaagagg gcctgagctg catgaataag      540

gatcacggct gtagtcacat ctgcaaggag gccccaaggg gcagcgtcgc ctgtgagtgc      600

aggcctggtt ttgagctggc caagaaccag agagactgca tcttgacctg taaccatggg      660

aacggtgggt gccagcactc ctgtgacgat acagccgatg gcccagagtg cagctgccat      720

ccacagtaca agatgcacac agatgggagg agctgccttg agcgagagga cactgtcctg      780

gaggtgacag agagcaacac cacatcagtg gtggatgggg ataaacgggt gaaacggcgg      840

ctgctcatgg aaacgtgtgc tgtcaacaat ggaggctgtg accgcacctg taaggatact      900

tcgacaggtg tccactgcag ttgtcctgtt ggattcactc tccagttgga tgggaagaca      960

tgtaaagata ttgatgagtg ccagacccgc aatggaggtt gtgatcattt ctgcaaaaac     1020

atcgtgggca gttttgactg cggctgcaag aaaggattta aattattaac agatgagaag     1080

tcttgccaag atgtggatga gtgctctttg gataggacct gtgaccacag ctgcatcaac     1140

caccctggca catttgcttg tgcttgcaac cgagggtaca ccctgtatgg cttcacccac     1200

tgtggagaca ccaatgagtg cagcatcaac aacggaggct gtcagcaggt ctgtgtgaac     1260

acagtgggca gctatgaatg ccagtgccac cctgggtaca agctccactg gaataaaaaa     1320

gactgtgtgg aagtgaaggg gctcctgccc acaagtgtgt caccccgtgt gtccctgcac     1380

tgcggtaaga gtggtggagg agacgggtgc ttcctcagat gtcactctgg cattcacctc     1440

tcttcaggac tgcaaggggc ctactctgtc acctgtggct cttcctctcc tctcaggaac     1500

aaacaacaaa aatcaaatga ctctgctttt ggggatgtca ccaccatcag gacaagtgta     1560

acctttaagc taaatgaagg caagtgtagt ttgaaaaatg ctgagctgtt tcccgagggt     1620

ctgcgaccag cactaccaga gaagcacagc tcagtaaaag agagcttccg ctacgtaaac     1680

cttacatgca gctctggcaa gcaagtccca ggagcccctg gccgaccaag cacccctaag     1740

gaaatgttta tcactgttga gtttgagctt gaaactaacc aaaaggaggt gacagcttct     1800

tgtgacctga gctgcatcgt aaagcgaacc gagaagcggc tccgtaaagc catccgcacg     1860

ctcagaaagg ccgtccacag ggagcagttt cacctccagc tctcaggcat gaacctcgac     1920

gtggctaaaa agcctcccag aacatctgaa cgccaggcag agtcctgtgg agtgggccag     1980

ggtcatgcag aaaaccaatg tgtcagttgc agggctggga cctattatga tggagcacga     2040

gaacgctgca ttttatgtcc aaatggaacc ttccaaaatg aggaaggaca aatgacttgt     2100

gaaccatgcc caagaccagg aaattctggg gccctgaaga ccccagaagc ttggaatatg     2160

tctgaatgtg gaggtctgtg tcaacctggt gaatattctg cagatggctt tgcaccttgc     2220

cagctctgtg ccctgggcac gttccagcct gaagctggtc gaacttcctg cttcccctgt     2280

ggaggaggcc ttgccaccaa acatcaggga gctacttcct ttcaggactg tgaaaccaga     2340

gttcaatgtt cacctggaca tttctacaac accaccactc accgatgtat tcgttgccca     2400

gtgggaacat accagcctga atttggaaaa aataattgtg tttcttgccc aggaaatact     2460

acgactgact ttgatggctc cacaaacata acccagtgta aaaacagaag atgtggaggg     2520

gagctgggag atttcactgg gtacattgaa tccccaaact acccaggcaa ttacccagcc     2580

aacaccgagt gtacgtggac catcaaccca ccccccaagc gccgcatcct gatcgtggtc     2640

cctgagatct tcctgcccat agaggacgac tgtggggact atctggtgat gcggaaaacc     2700

tcttctccaa ttctgtgaca acatatgaaa cctgccagac ctacgaacgc cccatcgcct     2760

tcacctccag gtcaaagaag ctgtggattc agttcaagtc caatgaaggg aacagcgcta     2820

gagggttcca ggtcccatac gtgacatatg atgaggacta ccaggaactc attgaagaca     2880

tagttcgaga tggcaggctc tatgcatctg agaaccatca ggaaatactt aaggataaga     2940

aacttatcaa ggctctgttt gatgtcctgg cccatcccca gaactatttc aagtacacag     3000

cccaggagtc ccgagagatg tttccaagat cgttcatccg attgctacgt tccaaagtgt     3060

ccaggttttt gagaccttac aaatga                                          3086


<210>  66
<211>  1028
<212>  PRT
<213>  Homo sapiens

<400>  66

Met Gly Val Ala Gly Arg Asn Arg Pro Gly Ala Ala Trp Ala Val Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Leu Pro Pro Leu Leu Leu Leu Ala Gly Ala Val 
            20                  25                  30          


Pro Pro Gly Arg Gly Arg Ala Ala Gly Pro Gln Glu Asp Val Asp Glu 
        35                  40                  45              


Cys Ala Gln Gly Leu Asp Asp Cys His Ala Asp Ala Leu Cys Gln Asn 
    50                  55                  60                  


Thr Pro Thr Ser Tyr Lys Cys Ser Cys Lys Pro Gly Tyr Gln Gly Glu 
65                  70                  75                  80  


Gly Arg Gln Cys Glu Asp Ile Asp Glu Cys Gly Asn Glu Leu Asn Gly 
                85                  90                  95      


Gly Cys Val His Asp Cys Leu Asn Ile Pro Gly Asn Tyr Arg Cys Thr 
            100                 105                 110         


Cys Phe Asp Gly Phe Met Leu Ala His Asp Gly His Asn Cys Leu Asp 
        115                 120                 125             


Val Asp Glu Cys Leu Glu Asn Asn Gly Gly Cys Gln His Thr Cys Val 
    130                 135                 140                 


Asn Val Met Gly Ser Tyr Glu Cys Cys Cys Lys Glu Gly Phe Phe Leu 
145                 150                 155                 160 


Ser Asp Asn Gln His Thr Cys Ile His Arg Ser Glu Glu Gly Leu Ser 
                165                 170                 175     


Cys Met Asn Lys Asp His Gly Cys Ser His Ile Cys Lys Glu Ala Pro 
            180                 185                 190         


Arg Gly Ser Val Ala Cys Glu Cys Arg Pro Gly Phe Glu Leu Ala Lys 
        195                 200                 205             


Asn Gln Arg Asp Cys Ile Leu Thr Cys Asn His Gly Asn Gly Gly Cys 
    210                 215                 220                 


Gln His Ser Cys Asp Asp Thr Ala Asp Gly Pro Glu Cys Ser Cys His 
225                 230                 235                 240 


Pro Gln Tyr Lys Met His Thr Asp Gly Arg Ser Cys Leu Glu Arg Glu 
                245                 250                 255     


Asp Thr Val Leu Glu Val Thr Glu Ser Asn Thr Thr Ser Val Val Asp 
            260                 265                 270         


Gly Asp Lys Arg Val Lys Arg Arg Leu Leu Met Glu Thr Cys Ala Val 
        275                 280                 285             


Asn Asn Gly Gly Cys Asp Arg Thr Cys Lys Asp Thr Ser Thr Gly Val 
    290                 295                 300                 


His Cys Ser Cys Pro Val Gly Phe Thr Leu Gln Leu Asp Gly Lys Thr 
305                 310                 315                 320 


Cys Lys Asp Ile Asp Glu Cys Gln Thr Arg Asn Gly Gly Cys Asp His 
                325                 330                 335     


Phe Cys Lys Asn Ile Val Gly Ser Phe Asp Cys Gly Cys Lys Lys Gly 
            340                 345                 350         


Phe Lys Leu Leu Thr Asp Glu Lys Ser Cys Gln Asp Val Asp Glu Cys 
        355                 360                 365             


Ser Leu Asp Arg Thr Cys Asp His Ser Cys Ile Asn His Pro Gly Thr 
    370                 375                 380                 


Phe Ala Cys Ala Cys Asn Arg Gly Tyr Thr Leu Tyr Gly Phe Thr His 
385                 390                 395                 400 


Cys Gly Asp Thr Asn Glu Cys Ser Ile Asn Asn Gly Gly Cys Gln Gln 
                405                 410                 415     


Val Cys Val Asn Thr Val Gly Ser Tyr Glu Cys Gln Cys His Pro Gly 
            420                 425                 430         


Tyr Lys Leu His Trp Asn Lys Lys Asp Cys Val Glu Val Lys Gly Leu 
        435                 440                 445             


Leu Pro Thr Ser Val Ser Pro Arg Val Ser Leu His Cys Gly Lys Ser 
    450                 455                 460                 


Gly Gly Gly Asp Gly Cys Phe Leu Arg Cys His Ser Gly Ile His Leu 
465                 470                 475                 480 


Ser Ser Gly Leu Gln Gly Ala Tyr Ser Val Thr Cys Gly Ser Ser Ser 
                485                 490                 495     


Pro Leu Arg Asn Lys Gln Gln Lys Ser Asn Asp Ser Ala Phe Gly Asp 
            500                 505                 510         


Val Thr Thr Ile Arg Thr Ser Val Thr Phe Lys Leu Asn Glu Gly Lys 
        515                 520                 525             


Cys Ser Leu Lys Asn Ala Glu Leu Phe Pro Glu Gly Leu Arg Pro Ala 
    530                 535                 540                 


Leu Pro Glu Lys His Ser Ser Val Lys Glu Ser Phe Arg Tyr Val Asn 
545                 550                 555                 560 


Leu Thr Cys Ser Ser Gly Lys Gln Val Pro Gly Ala Pro Gly Arg Pro 
                565                 570                 575     


Ser Thr Pro Lys Glu Met Phe Ile Thr Val Glu Phe Glu Leu Glu Thr 
            580                 585                 590         


Asn Gln Lys Glu Val Thr Ala Ser Cys Asp Leu Ser Cys Ile Val Lys 
        595                 600                 605             


Arg Thr Glu Lys Arg Leu Arg Lys Ala Ile Arg Thr Leu Arg Lys Ala 
    610                 615                 620                 


Val His Arg Glu Gln Phe His Leu Gln Leu Ser Gly Met Asn Leu Asp 
625                 630                 635                 640 


Val Ala Lys Lys Pro Pro Arg Thr Ser Glu Arg Gln Ala Glu Ser Cys 
                645                 650                 655     


Gly Val Gly Gln Gly His Ala Glu Asn Gln Cys Val Ser Cys Arg Ala 
            660                 665                 670         


Gly Thr Tyr Tyr Asp Gly Ala Arg Glu Arg Cys Ile Leu Cys Pro Asn 
        675                 680                 685             


Gly Thr Phe Gln Asn Glu Glu Gly Gln Met Thr Cys Glu Pro Cys Pro 
    690                 695                 700                 


Arg Pro Gly Asn Ser Gly Ala Leu Lys Thr Pro Glu Ala Trp Asn Met 
705                 710                 715                 720 


Ser Glu Cys Gly Gly Leu Cys Gln Pro Gly Glu Tyr Ser Ala Asp Gly 
                725                 730                 735     


Phe Ala Pro Cys Gln Leu Cys Ala Leu Gly Thr Phe Gln Pro Glu Ala 
            740                 745                 750         


Gly Arg Thr Ser Cys Phe Pro Cys Gly Gly Gly Leu Ala Thr Lys His 
        755                 760                 765             


Gln Gly Ala Thr Ser Phe Gln Asp Cys Glu Thr Arg Val Gln Cys Ser 
    770                 775                 780                 


Pro Gly His Phe Tyr Asn Thr Thr Thr His Arg Cys Ile Arg Cys Pro 
785                 790                 795                 800 


Val Gly Thr Tyr Gln Pro Glu Phe Gly Lys Asn Asn Cys Val Ser Cys 
                805                 810                 815     


Pro Gly Asn Thr Thr Thr Asp Phe Asp Gly Ser Thr Asn Ile Thr Gln 
            820                 825                 830         


Cys Lys Asn Arg Arg Cys Gly Gly Glu Leu Gly Asp Phe Thr Gly Tyr 
        835                 840                 845             


Ile Glu Ser Pro Asn Tyr Pro Gly Asn Tyr Pro Ala Asn Thr Glu Cys 
    850                 855                 860                 


Thr Trp Thr Ile Asn Pro Pro Pro Lys Arg Arg Ile Leu Ile Val Val 
865                 870                 875                 880 


Pro Glu Ile Phe Leu Pro Ile Glu Asp Asp Cys Gly Asp Tyr Leu Val 
                885                 890                 895     


Met Arg Lys Thr Ser Ser Ser Asn Ser Val Thr Thr Tyr Glu Thr Cys 
            900                 905                 910         


Gln Thr Tyr Glu Arg Pro Ile Ala Phe Thr Ser Arg Ser Lys Lys Leu 
        915                 920                 925             


Trp Ile Gln Phe Lys Ser Asn Glu Gly Asn Ser Ala Arg Gly Phe Gln 
    930                 935                 940                 


Val Pro Tyr Val Thr Tyr Asp Glu Asp Tyr Gln Glu Leu Ile Glu Asp 
945                 950                 955                 960 


Ile Val Arg Asp Gly Arg Leu Tyr Ala Ser Glu Asn His Gln Glu Ile 
                965                 970                 975     


Leu Lys Asp Lys Lys Leu Ile Lys Ala Leu Phe Asp Val Leu Ala His 
            980                 985                 990         


Pro Gln Asn Tyr Phe Lys Tyr Thr  Ala Gln Glu Ser Arg  Glu Met Phe 
        995                 1000                 1005             


Pro Arg  Ser Phe Ile Arg Leu  Leu Arg Ser Lys Val  Ser Arg Phe 
    1010                 1015                 1020             


Leu Arg  Pro Tyr Lys 
    1025             


<210>  67
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGF cDNA Forward primer

<400>  67
ccgctcgagg cacccatggc agaagga                                           27


<210>  68
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VEGF cDNA Reverse primer

<400>  68
gctctagatt atcaccgcct cggcttgtca ca                                     32


<210>  69
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE1 Forward

<400>  69
tgcggcggcg agcttggtga c                                                 21


<210>  70
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE1 Reverse

<400>  70
tttggagcgc agcagtttga tgaa                                              24


<210>  71
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE2 Forward

<400>  71
tcttgcccag gaaatactac gact                                              24


<210>  72
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE2 Reverse

<400>  72
tgggccagga catcaaacag ag                                                22


<210>  73
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE3 Forward

<400>  73
tggcccaatg caagaatcgt cagt                                              24


<210>  74
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE3 Reverse

<400>  74
tgggctagca cctcaaagaa g                                                 21


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH Forward

<400>  75
gccaaaaggg tcatcatctc                                                   20


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH Reverse

<400>  76
accacctggt gctcagtgta                                                   20


<210>  77
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Scube1 Forward

<400>  77
cggcggcgaa cttggtgact aca                                               23


<210>  78
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Scube1 Reverse

<400>  78
ttgataaagg accgggggaa cat                                               23


<210>  79
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Scube2 Forward

<400>  79
tgactacctg gtgatgcgga aaac                                              24


<210>  80
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Scube2 Reverse

<400>  80
cagtggcgtg tgggaagagt ca                                                22


<210>  81
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Scube3 Forward

<400>  81
tgctccccgg gccactacta t                                                 21


<210>  82
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Scube3 Reverse

<400>  82
agcgctgttg gcctcactgg tctt                                              24


<210>  83
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE2 Forward

<400>  83
gcacacgcac gcgcgcacac a                                                 21


<210>  84
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE2 Reverse

<400>  84
gaagggtgca gagggtgtgc t                                                 21


<210>  85
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Gapdh Forward

<400>  85
atcatccctg catccactgg tgctg                                             25


<210>  86
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mouse Gapdh Reverse

<400>  86
tgatggcatt caagagagta gggag                                             25


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE1 Forward

<400>  87
aacatcccgg ggaactacag                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE1 Reverse

<400>  88
gcagccacca ttattgtcct                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE2 Forward

<400>  89
caggcagagt cctgtggagt                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE2 Reverse

<400>  90
taaaatgcag cgttctcgtg                                                   20


<210>  91
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE3 Forward

<400>  91
cctgcttgtc ctgctggt                                                     18


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCUBE3 Reverse

<400>  92
tcgatgtggc agttgtcagt                                                   20


<210>  93
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH Forward

<400>  93
tgaaggtcgg agtcaacgg                                                    19


<210>  94
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH Reverse

<400>  94
agagttaaaa gcagccctgg tg                                                22


<210>  95
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scube2-5'Flox F1

<400>  95
atagtcgact gttgtccagt atctgttgc                                         29


<210>  96
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scube2-5'Flox R1

<400>  96
atagcggccg ctacgacacc ctgggataaa g                                      31


<210>  97
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scube2-3'Flox F2

<400>  97
ggccatgtcc ctgaagaaaa cta                                               23


<210>  98
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scube2-3'Flox R2

<400>  98
ttatggggcc aagacactca aa                                                22


<210>  99
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scube2-Null F3

<400>  99
ctggggcctc tgggacacta tt                                                22


<210>  100
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scube2-Null R3

<400>  100
gttatggggc caagacactc aaa                                               23


<210>  101
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cre Forward

<400>  101
ttaccggtcg atgcaacgag tgatg                                             25


<210>  102
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cre Reverse

<400>  102
gtgaaacagc attgctgtca ctt                                               23


