                         SEQUENCE LISTING

<110>  Novozymes A/S
       Blazej, Robert
       Toriello, Nicholas
       Emrich, Charles
 
<120>  Methods for Selecting Enzymes Having Protease Activity

<130>  13103-WO-PCT

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  1156
<212>  DNA
<213>  Bacillus lentus

<400>  1
cggtttcttg gcctccatat actaatacga ctcactatag ggagaccaca agaaggagat       60

atacatatgg ctgaagaagc aaaagaaaaa tatttaattg gctttaatga gcaggaagct      120

gtcagtgagt ttgtagaaca agtagaggca aatgacgagg tcgccattct ctctgaggaa      180

gaggaagtcg aaattgaatt gcttcatgaa tttgaaacga ttcctgtttt atccgttgag      240

ttaagcccag aagatgtgga cgcgcttgaa ctcgatccag cgatttctta tattgaagag      300

gatgcagaag taacgacaat ggcgcaatcg gtaccatggg gaattagccg tgtgcaagcc      360

ccagctgccc ataaccgtgg attgacaggt tctggtgtaa aagttgctgt cctcgataca      420

gggatatcca ctcatccaga tctaaatatt cgtggtggcg caagctttgt accaggggaa      480

ccgtcgactc aagatgggaa tgggcatggc acgcatgtgg ccgggacgat cgctgcttta      540

aacaattcga ttggcgttct tggcgtagct cctagcgctg agctatacgc tgttaaagtc      600

ctaggggcga gcggttcagg ttcggtcagc tcgattgccc aaggattgga atgggcaggg      660

aacaatggca tgcacgttgc taatttgagt ttaggaagcc cttcgccaag tgccacactc      720

gagcaagctg ttaatagcgc gacttctaga ggcgttcttg ttgtagcggc atctgggaat      780

tcaggtgcag gctcaatcag ctatccggcg cgctatgcga acgcaatggc agtcggagct      840

actgatcaaa acaacaaccg cgctagcttt tcacagtatg gcgcaggcct tgacattgtc      900

gcacccgggg taaacgtgca gagcacatac ccaggttcaa catatgccag cttaaacggt      960

acatcgatgg ctactcctca tgttgcaggt gcggccgccc ttgttaaaca aaagaaccca     1020

tcttggtcta atgtacaaat tcgaaatcat ctaaagaata cggcaactag tttaggaagc     1080

acgaacttgt atggaagcgg acttgttaac gcagaagcgg caacgcgtta ataactataa     1140

gcgcacacgt actgac                                                     1156


<210>  2
<211>  1065
<212>  DNA
<213>  Bacillus lentus

<400>  2
atggctgaag aagcaaaaga aaaatattta attggcttta atgagcagga agctgtcagt       60

gagtttgtag aacaagtaga ggcaaatgac gaggtcgcca ttctctctga ggaagaggaa      120

gtcgaaattg aattgcttca tgaatttgaa acgattcctg ttttatccgt tgagttaagc      180

ccagaagatg tggacgcgct tgaactcgat ccagcgattt cttatattga agaggatgca      240

gaagtaacga caatggcgca atcggtacca tggggaatta gccgtgtgca agccccagct      300

gcccataacc gtggattgac aggttctggt gtaaaagttg ctgtcctcga tacagggata      360

tccactcatc cagatctaaa tattcgtggt ggcgcaagct ttgtaccagg ggaaccgtcg      420

actcaagatg ggaatgggca tggcacgcat gtggccggga cgatcgctgc tttaaacaat      480

tcgattggcg ttcttggcgt agctcctagc gctgagctat acgctgttaa agtcctaggg      540

gcgagcggtt caggttcggt cagctcgatt gcccaaggat tggaatgggc agggaacaat      600

ggcatgcacg ttgctaattt gagtttagga agcccttcgc caagtgccac actcgagcaa      660

gctgttaata gcgcgacttc tagaggcgtt cttgttgtag cggcatctgg gaattcaggt      720

gcaggctcaa tcagctatcc ggcgcgctat gcgaacgcaa tggcagtcgg agctactgat      780

caaaacaaca accgcgctag cttttcacag tatggcgcag gccttgacat tgtcgcaccc      840

ggggtaaacg tgcagagcac atacccaggt tcaacatatg ccagcttaaa cggtacatcg      900

atggctactc ctcatgttgc aggtgcggcc gcccttgtta aacaaaagaa cccatcttgg      960

tctaatgtac aaattcgaaa tcatctaaag aatacggcaa ctagtttagg aagcacgaac     1020

ttgtatggaa gcggacttgt taacgcagaa gcggcaacgc gttaa                     1065


<210>  3
<211>  354
<212>  PRT
<213>  Bacillus lentus

<400>  3

Met Ala Glu Glu Ala Lys Glu Lys Tyr Leu Ile Gly Phe Asn Glu Gln 
1               5                   10                  15      


Glu Ala Val Ser Glu Phe Val Glu Gln Val Glu Ala Asn Asp Glu Val 
            20                  25                  30          


Ala Ile Leu Ser Glu Glu Glu Glu Val Glu Ile Glu Leu Leu His Glu 
        35                  40                  45              


Phe Glu Thr Ile Pro Val Leu Ser Val Glu Leu Ser Pro Glu Asp Val 
    50                  55                  60                  


Asp Ala Leu Glu Leu Asp Pro Ala Ile Ser Tyr Ile Glu Glu Asp Ala 
65                  70                  75                  80  


Glu Val Thr Thr Met Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val 
                85                  90                  95      


Gln Ala Pro Ala Ala His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys 
            100                 105                 110         


Val Ala Val Leu Asp Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile 
        115                 120                 125             


Arg Gly Gly Ala Ser Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly 
    130                 135                 140                 


Asn Gly His Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn 
145                 150                 155                 160 


Ser Ile Gly Val Leu Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val 
                165                 170                 175     


Lys Val Leu Gly Ala Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln 
            180                 185                 190         


Gly Leu Glu Trp Ala Gly Asn Asn Gly Met His Val Ala Asn Leu Ser 
        195                 200                 205             


Leu Gly Ser Pro Ser Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser 
    210                 215                 220                 


Ala Thr Ser Arg Gly Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly 
225                 230                 235                 240 


Ala Gly Ser Ile Ser Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val 
                245                 250                 255     


Gly Ala Thr Asp Gln Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly 
            260                 265                 270         


Ala Gly Leu Asp Ile Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr 
        275                 280                 285             


Pro Gly Ser Thr Tyr Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro 
    290                 295                 300                 


His Val Ala Gly Ala Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp 
305                 310                 315                 320 


Ser Asn Val Gln Ile Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu 
                325                 330                 335     


Gly Ser Thr Asn Leu Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala 
            340                 345                 350         


Thr Arg 
        


<210>  4
<211>  1156
<212>  DNA
<213>  Bacillus lentus

<400>  4
cggtttcttg gcctccatat actaatacga ctcactatag ggagaccaca agaaggagat       60

atacatatgg ctgaagaagc aaaagaaaaa tatttaattg gctttaatga gcaggaagct      120

gtcagtgagt ttgtagaaca agtagaggca aatgacgagg tcgccattct ctctgaggaa      180

gaggaagtcg aaattgaatt gcttcatgaa tttgaaacga ttcctgtttt atccgttgag      240

ttaagcccag aagatgtgga cgcgcttgaa ctcgatccag cgatttctta tattgaagag      300

gatgcagaag taacgacaat ggcgcaatcg gtaccatggg gaattagccg tgtgcaagcc      360

ccagctgccc ataaccgtgg attgacaggt tctggtgtaa aagttgctgt cctcgataca      420

gggatatcca ctcatccaga tctaaatatt cgtggtggcg caagctttgt accaggggaa      480

ccgtcgactc aagatgggaa tgggcatggc acgcatgtgg ccgggacgat cgctgcttta      540

aacaattcga ttggcgttct tggcgtagct cctagcgctg agctatacgc tgttaaagtc      600

ctaggggcga gcggttcagg ttcggtcagc tcgattgccc aaggattgga atgggcaggg      660

aacaatggca tgcacgttgc taatttgagt ttaggaagcc cttcgccaag tgccacactc      720

gagcaagctg ttaatagcgc gacttctaga ggcgttcttg ttgtagcggc atctgggaat      780

tcaggtgcag gctcaatcag ctatccggcg cgctatgcga acgcaatggc agtcggagct      840

actgatcaaa acaacaaccg cgctagcttt tcacagtatg gcgcaggcct tgacattgtc      900

gcacccgggg taaacgtgca gagcacatac ccaggttcaa catatgccag cttaaacggt      960

acagcaatgg ctactcctca tgttgcaggt gcggccgccc ttgttaaaca aaagaaccca     1020

tcttggtcta atgtacaaat tcgaaatcat ctaaagaata cggcaactag tttaggaagc     1080

acgaacttgt atggaagcgg acttgttaac gcagaagcgg caacgcgtta ataactataa     1140

gcgcacacgt actgac                                                     1156


<210>  5
<211>  941
<212>  DNA
<213>  Bacillus lentus

<400>  5
atggctgaag aagcaaaaga aaaatattta attggcttta atgagcagga agctgtcagt       60

gagtttgtag aacaagtaga ggcaaatgac gaggtcgcca ttctctctga ggaagaggaa      120

gtcgaaattg aattgcttca tgaatttgaa acgattcctg ttttatccgt tgagttaagc      180

ccagaagatg tggacgcgct tgaactcgat ccagcgattt cttatattga agaggatgca      240

gaagtaacga caatggcgca atcggtacca tggggaatta gccgtgtgca agccccagct      300

gcccataacc gtggattgac aggttctggt gtaaaagttg ctgtcctcga tacagggata      360

tccactcatc cagatctaaa tattcgtggt ggcgcaagct ttgtaccagg ggaaccgtcg      420

actcaagatg ggaatgggca tggcacgcat gtggccggga cgatcgctgc tttaaacaat      480

tcgattggcg ttcttggcgt agctcctagc gctgagctat acgctgttaa agtcctaggg      540

gcgagcggtt caggttcggt cagctcgatt gcccaaggat tggaatgggc agggaacaat      600

ggcatgcacg ttgctaattt gagtttagga agcccttcgc caagtgccac actcgagcaa      660

gctgttaata gcgcgacttc tagaggcgtt cttgttgtag cggcatctgg gaattcaggt      720

gcaggctcaa tcagctatcc ggcgcgctat gcgaacgcaa tggcagtcgg agctactgat      780

caaaacaaca accgcgctag cttttcacag tatggcgcag gccttgacat tgtcgcaccc      840

ggggtaaacg tgcagagcac atacccaggt tcaacatatg ccagcttaaa cggtacagca      900

atggctactc ctcatgttgc aggtgcggcc gcccttgtta a                          941


<210>  6
<211>  312
<212>  PRT
<213>  Bacillus lentus

<400>  6

Met Ala Glu Glu Ala Lys Glu Lys Tyr Leu Ile Gly Phe Asn Glu Gln 
1               5                   10                  15      


Glu Ala Val Ser Glu Phe Val Glu Gln Val Glu Ala Asn Asp Glu Val 
            20                  25                  30          


Ala Ile Leu Ser Glu Glu Glu Glu Val Glu Ile Glu Leu Leu His Glu 
        35                  40                  45              


Phe Glu Thr Ile Pro Val Leu Ser Val Glu Leu Ser Pro Glu Asp Val 
    50                  55                  60                  


Asp Ala Leu Glu Leu Asp Pro Ala Ile Ser Tyr Ile Glu Glu Asp Ala 
65                  70                  75                  80  


Glu Val Thr Thr Met Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val 
                85                  90                  95      


Gln Ala Pro Ala Ala His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys 
            100                 105                 110         


Val Ala Val Leu Asp Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile 
        115                 120                 125             


Arg Gly Gly Ala Ser Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly 
    130                 135                 140                 


Asn Gly His Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn 
145                 150                 155                 160 


Ser Ile Gly Val Leu Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val 
                165                 170                 175     


Lys Val Leu Gly Ala Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln 
            180                 185                 190         


Gly Leu Glu Trp Ala Gly Asn Asn Gly Met His Val Ala Asn Leu Ser 
        195                 200                 205             


Leu Gly Ser Pro Ser Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser 
    210                 215                 220                 


Ala Thr Ser Arg Gly Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly 
225                 230                 235                 240 


Ala Gly Ser Ile Ser Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val 
                245                 250                 255     


Gly Ala Thr Asp Gln Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly 
            260                 265                 270         


Ala Gly Leu Asp Ile Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr 
        275                 280                 285             


Pro Gly Ser Thr Tyr Ala Ser Leu Asn Gly Thr His Gly Tyr Ser Ser 
    290                 295                 300                 


Cys Cys Arg Cys Gly Arg Pro Cys 
305                 310         


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  7
cggtttcttg gcctccatat ac                                                22


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  8
aagtcagtac gtgtgcgctt atag                                              24


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  9
gtcagtacgt gtgcgcttat ag                                                22


<210>  10
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  10
cagaacgtca ctctctcttc actaatacga ctcactatag ggagaccaca agaaggagat       60

atacatatgg ctgaagaagc aaaagaaaaa                                        90


<210>  11
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  11
cagaacgtca ctctctcttc actaatacga ctcactatag ggagaccaca agaaggagat       60

atacatatgg cgcaatcggt accatgg                                           87


<210>  12
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  12
gctcacctgg gctatgtatt agttattaac gcgttgccgc ttctgc                      46


<210>  13
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  13
cagaacgtca ctctctcttc ac                                                22


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  14
aaaaaacgga gcgaaccact tatc                                              24


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  15
ggcatgcacg ttgctaattt                                                   20


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  16
gctacaacaa gaacgcctct a                                                 21


<210>  17
<211>  275
<212>  PRT
<213>  Bacillus amyloliquefaciens

<400>  17

Ala Gln Ser Val Pro Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      


His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          


Ser Gly Ile Asp Ser Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala 
        35                  40                  45              


Ser Met Val Pro Ser Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  


Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      


Gly Ala Asp Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         


Trp Ala Ile Ala Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             


Pro Ser Gly Ser Ala Ala Leu Lys Ala Ala Val Asp Lys Ala Val Ala 
    130                 135                 140                 


Ser Gly Val Val Val Val Ala Ala Ala Gly Asn Glu Gly Thr Ser Gly 
145                 150                 155                 160 


Ser Ser Ser Thr Val Gly Tyr Pro Gly Lys Tyr Pro Ser Val Ile Ala 
                165                 170                 175     


Val Gly Ala Val Asp Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Val 
            180                 185                 190         


Gly Pro Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             


Leu Pro Gly Asn Lys Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Ser 
    210                 215                 220                 


Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn 
225                 230                 235                 240 


Trp Thr Asn Thr Gln Val Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys 
                245                 250                 255     


Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         


Ala Ala Gln 
        275 


<210>  18
<211>  275
<212>  PRT
<213>  Bacillus subtilis

<400>  18

Ala Gln Ser Val Pro Tyr Gly Ile Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      


His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          


Ser Gly Ile Asp Ser Ser His Pro Asp Leu Asn Val Arg Gly Gly Ala 
        35                  40                  45              


Ser Phe Val Pro Ser Glu Thr Asn Pro Tyr Gln Asp Gly Ser Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  


Val Leu Gly Val Ser Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      


Asp Ser Thr Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         


Trp Ala Ile Ser Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             


Pro Thr Gly Ser Thr Ala Leu Lys Thr Val Val Asp Lys Ala Val Ser 
    130                 135                 140                 


Ser Gly Ile Val Val Ala Ala Ala Ala Gly Asn Glu Gly Ser Ser Gly 
145                 150                 155                 160 


Ser Thr Ser Thr Val Gly Tyr Pro Ala Lys Tyr Pro Ser Thr Ile Ala 
                165                 170                 175     


Val Gly Ala Val Asn Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Ala 
            180                 185                 190         


Gly Ser Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             


Leu Pro Gly Gly Thr Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Thr 
    210                 215                 220                 


Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Thr 
225                 230                 235                 240 


Trp Thr Asn Ala Gln Val Arg Asp Arg Leu Glu Ser Thr Ala Thr Tyr 
                245                 250                 255     


Leu Gly Asn Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         


Ala Ala Gln 
        275 


<210>  19
<211>  274
<212>  PRT
<213>  Bacillus subtilis

<400>  19

Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      


Gln Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Gly Ile Ile Asp 
            20                  25                  30          


Thr Gly Ile Ala Ala Ser His Thr Asp Leu Lys Val Val Gly Gly Ala 
        35                  40                  45              


Ser Phe Val Ser Gly Glu Ser Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  


Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  


Leu Gly Val Ala Pro Asn Val Ser Leu Tyr Ala Ile Lys Val Leu Asn 
                85                  90                  95      


Ser Ser Gly Ser Gly Thr Tyr Ser Ala Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         


Ala Thr Gln Asn Gly Leu Asp Val Ile Asn Met Ser Leu Gly Gly Pro 
        115                 120                 125             


Ser Gly Ser Thr Ala Leu Lys Gln Ala Val Asp Lys Ala Tyr Ala Ser 
    130                 135                 140                 


Gly Ile Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Ser 
145                 150                 155                 160 


Gln Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     


Gly Ala Val Asp Ser Asn Lys Asn Arg Ala Ser Phe Ser Ser Val Gly 
            180                 185                 190         


Ala Glu Leu Glu Val Met Ala Pro Gly Val Ser Val Tyr Ser Thr Tyr 
        195                 200                 205             


Pro Ser Asn Thr Tyr Thr Ser Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 


His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys Tyr Pro Thr Leu 
225                 230                 235                 240 


Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Asn Leu 
                245                 250                 255     


Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         


Ala Gln 
        


<210>  20
<211>  274
<212>  PRT
<213>  Bacillus licheniformis

<400>  20

Ala Gln Thr Val Pro Tyr Gly Ile Pro Leu Ile Lys Ala Asp Lys Val 
1               5                   10                  15      


Gln Ala Gln Gly Phe Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Gln Ala Ser His Pro Asp Leu Asn Val Val Gly Gly Ala 
        35                  40                  45              


Ser Phe Val Ala Gly Glu Ala Tyr Asn Thr Asp Gly Asn Gly His Gly 
    50                  55                  60                  


Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Thr Thr Gly Val 
65                  70                  75                  80  


Leu Gly Val Ala Pro Ser Val Ser Leu Tyr Ala Val Lys Val Leu Asn 
                85                  90                  95      


Ser Ser Gly Ser Gly Thr Tyr Ser Gly Ile Val Ser Gly Ile Glu Trp 
            100                 105                 110         


Ala Thr Thr Asn Gly Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro 
        115                 120                 125             


Ser Gly Ser Thr Ala Met Lys Gln Ala Val Asp Asn Ala Tyr Ala Arg 
    130                 135                 140                 


Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly Asn 
145                 150                 155                 160 


Thr Asn Thr Ile Gly Tyr Pro Ala Lys Tyr Asp Ser Val Ile Ala Val 
                165                 170                 175     


Gly Ala Val Asp Ser Asn Ser Asn Arg Ala Ser Phe Ser Ser Val Gly 
            180                 185                 190         


Ala Glu Leu Glu Val Met Ala Pro Gly Ala Gly Val Tyr Ser Thr Tyr 
        195                 200                 205             


Pro Thr Ser Thr Tyr Ala Thr Leu Asn Gly Thr Ser Met Ala Ser Pro 
    210                 215                 220                 


His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn Leu 
225                 230                 235                 240 


Ser Ala Ser Gln Val Arg Asn Arg Leu Ser Ser Thr Ala Thr Tyr Leu 
                245                 250                 255     


Gly Ser Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Glu Ala Ala 
            260                 265                 270         


Ala Gln 
        


<210>  21
<211>  268
<212>  PRT
<213>  Bacillus lentus

<400>  21

Gln Thr Val Pro Trp Gly Ile Ser Phe Ile Asn Thr Gln Gln Ala His 
1               5                   10                  15      


Asn Arg Gly Ile Phe Gly Asn Gly Ala Arg Val Ala Val Leu Asp Thr 
            20                  25                  30          


Gly Ile Ala Ser His Pro Asp Leu Arg Ile Ala Gly Gly Ala Ser Phe 
        35                  40                  45              


Ile Ser Ser Glu Pro Ser Tyr His Asp Asn Asn Gly His Gly Thr His 
    50                  55                  60                  


Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu Gly 
65                  70                  75                  80  


Val Arg Pro Ser Ala Asp Leu Tyr Ala Leu Lys Val Leu Asp Arg Asn 
                85                  90                  95      


Gly Ser Gly Ser Leu Ala Ser Val Ala Gln Gly Ile Glu Trp Ala Ile 
            100                 105                 110         


Asn Asn Asn Met His Ile Ile Asn Met Ser Leu Gly Ser Thr Ser Gly 
        115                 120                 125             


Ser Ser Thr Leu Glu Leu Ala Val Asn Arg Ala Asn Asn Ala Gly Ile 
    130                 135                 140                 


Leu Leu Val Gly Ala Ala Gly Asn Thr Gly Arg Gln Gly Val Asn Tyr 
145                 150                 155                 160 


Pro Ala Arg Tyr Ser Gly Val Met Ala Val Ala Ala Val Asp Gln Asn 
                165                 170                 175     


Gly Gln Arg Ala Ser Phe Ser Thr Tyr Gly Pro Glu Ile Glu Ile Ser 
            180                 185                 190         


Ala Pro Gly Val Asn Val Asn Ser Thr Tyr Thr Gly Asn Arg Tyr Val 
        195                 200                 205             


Ser Leu Ser Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val Ala 
    210                 215                 220                 


Ala Leu Val Lys Ser Arg Tyr Pro Ser Tyr Thr Asn Asn Gln Ile Arg 
225                 230                 235                 240 


Gln Arg Ile Asn Gln Thr Ala Thr Tyr Leu Gly Ser Pro Ser Leu Tyr 
                245                 250                 255     


Gly Asn Gly Leu Val His Ala Gly Arg Ala Thr Gln 
            260                 265             


<210>  22
<211>  269
<212>  PRT
<213>  Bacillus alcalophilus

<400>  22

Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Asn Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  23
<211>  268
<212>  PRT
<213>  Bacillus YaB

<400>  23

Gln Thr Val Pro Trp Gly Ile Asn Arg Val Gln Ala Pro Ile Ala Gln 
1               5                   10                  15      


Ser Arg Gly Phe Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp Thr 
            20                  25                  30          


Gly Ile Ser Asn His Ala Asp Leu Arg Ile Arg Gly Gly Ala Ser Phe 
        35                  40                  45              


Val Pro Gly Glu Pro Asn Ile Ser Asp Gly Asn Gly His Gly Thr Gln 
    50                  55                  60                  


Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu Gly 
65                  70                  75                  80  


Val Ala Pro Asn Val Asp Leu Tyr Gly Val Lys Val Leu Gly Ala Ser 
                85                  90                  95      


Gly Ser Gly Ser Ile Ser Gly Ile Ala Gln Gly Leu Gln Trp Ala Ala 
            100                 105                 110         


Asn Asn Gly Met His Ile Ala Asn Met Ser Leu Gly Ser Ser Ala Gly 
        115                 120                 125             


Ser Ala Thr Met Glu Gln Ala Val Asn Gln Ala Thr Ala Ser Gly Val 
    130                 135                 140                 


Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Asn Val Gly Phe 
145                 150                 155                 160 


Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln Asn 
                165                 170                 175     


Asn Asn Arg Ala Thr Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile Val 
            180                 185                 190         


Ala Pro Gly Val Gly Val Gln Ser Thr Val Pro Gly Asn Gly Tyr Ala 
        195                 200                 205             


Ser Phe Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val Ala 
    210                 215                 220                 


Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile Arg 
225                 230                 235                 240 


Asn His Leu Lys Asn Thr Ala Thr Asn Leu Gly Asn Thr Thr Gln Phe 
                245                 250                 255     


Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265             


<210>  24
<211>  272
<212>  PRT
<213>  Bacillus sp. NKS-21

<400>  24

Gln Thr Val Pro Trp Gly Ile Pro Tyr Ile Tyr Ser Asp Val Val His 
1               5                   10                  15      


Arg Gln Gly Tyr Phe Gly Asn Gly Val Lys Val Ala Val Leu Asp Thr 
            20                  25                  30          


Gly Val Ala Pro His Pro Asp Leu His Ile Arg Gly Gly Val Ser Phe 
        35                  40                  45              


Ile Ser Thr Glu Asn Thr Tyr Val Asp Tyr Asn Gly His Gly Thr His 
    50                  55                  60                  


Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Tyr Gly Val Leu Gly 
65                  70                  75                  80  


Val Ala Pro Gly Ala Glu Leu Tyr Ala Val Lys Val Leu Asp Arg Asn 
                85                  90                  95      


Gly Ser Gly Ser His Ala Ser Ile Ala Gln Gly Ile Glu Trp Ala Met 
            100                 105                 110         


Asn Asn Gly Met Asp Ile Ala Asn Met Ser Leu Gly Ser Pro Ser Gly 
        115                 120                 125             


Ser Thr Thr Leu Gln Leu Ala Ala Asp Arg Ala Arg Asn Ala Gly Val 
    130                 135                 140                 


Leu Leu Ile Gly Ala Ala Gly Asn Ser Gly Gln Gln Gly Gly Ser Asn 
145                 150                 155                 160 


Asn Met Gly Tyr Pro Ala Arg Tyr Ala Ser Val Met Ala Val Gly Ala 
                165                 170                 175     


Val Asp Gln Asn Gly Asn Arg Ala Asn Phe Ser Ser Tyr Gly Ser Glu 
            180                 185                 190         


Leu Glu Ile Met Ala Pro Gly Val Asn Ile Asn Ser Thr Tyr Leu Asn 
        195                 200                 205             


Asn Gly Tyr Arg Ser Leu Asn Gly Thr Ser Met Ala Ser Pro His Val 
    210                 215                 220                 


Ala Gly Val Ala Ala Leu Val Lys Gln Lys His Pro His Leu Thr Ala 
225                 230                 235                 240 


Ala Gln Ile Arg Asn Arg Met Asn Gln Thr Ala Ile Pro Leu Gly Asn 
                245                 250                 255     


Ser Thr Tyr Tyr Gly Asn Gly Leu Val Asp Ala Glu Tyr Ala Ala Gln 
            260                 265                 270         


<210>  25
<211>  269
<212>  PRT
<213>  Bacillus sp. G-825-6

<400>  25

Asn Gln Val Thr Pro Trp Gly Ile Thr Arg Val Gln Ala Pro Thr Ala 
1               5                   10                  15      


Trp Thr Arg Gly Tyr Thr Gly Thr Gly Val Arg Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Val Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Tyr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Val 
65                  70                  75                  80  


Gly Val Ala Pro Asn Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Asn Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Gln Trp Thr 
            100                 105                 110         


Ala Gln Asn Asn Ile His Val Ala Asn Leu Ser Leu Gly Ser Pro Val 
        115                 120                 125             


Gly Ser Gln Thr Leu Glu Leu Ala Val Asn Gln Ala Thr Asn Ala Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Thr Gly Asn Asn Gly Ser Gly Thr Val Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Leu Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Thr Gly Leu Asn Ile 
            180                 185                 190         


Val Ala Pro Gly Val Gly Ile Gln Ser Thr Tyr Pro Gly Asn Arg Tyr 
        195                 200                 205             


Ala Ser Leu Ser Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Val 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Thr Gln Ile 
225                 230                 235                 240 


Arg Gln His Leu Thr Ser Thr Ala Thr Ser Leu Gly Asn Ser Asn Gln 
                245                 250                 255     


Phe Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  26
<211>  279
<212>  PRT
<213>  Thermoactinomyces vulgaris

<400>  26

Tyr Thr Pro Asn Asp Pro Tyr Phe Ser Ser Arg Gln Tyr Gly Pro Gln 
1               5                   10                  15      


Lys Ile Gln Ala Pro Gln Ala Trp Asp Ile Ala Glu Gly Ser Gly Ala 
            20                  25                  30          


Lys Ile Ala Ile Val Asp Thr Gly Val Gln Ser Asn His Pro Asp Leu 
        35                  40                  45              


Ala Gly Lys Val Val Gly Gly Trp Asp Phe Val Asp Asn Asp Ser Thr 
    50                  55                  60                  


Pro Gln Asn Gly Asn Gly His Gly Thr His Cys Ala Gly Ile Ala Ala 
65                  70                  75                  80  


Ala Val Thr Asn Asn Ser Thr Gly Ile Ala Gly Thr Ala Pro Lys Ala 
                85                  90                  95      


Ser Ile Leu Ala Val Arg Val Leu Asp Asn Ser Gly Ser Gly Thr Trp 
            100                 105                 110         


Thr Ala Val Ala Asn Gly Ile Thr Tyr Ala Ala Asp Gln Gly Ala Lys 
        115                 120                 125             


Val Ile Ser Leu Ser Leu Gly Gly Thr Val Gly Asn Ser Gly Leu Gln 
    130                 135                 140                 


Gln Ala Val Asn Tyr Ala Trp Asn Lys Gly Ser Val Val Val Ala Ala 
145                 150                 155                 160 


Ala Gly Asn Ala Gly Asn Thr Ala Pro Asn Tyr Pro Ala Tyr Tyr Ser 
                165                 170                 175     


Asn Ala Ile Ala Val Ala Ser Thr Asp Gln Asn Asp Asn Lys Ser Ser 
            180                 185                 190         


Phe Ser Thr Tyr Gly Ser Val Val Asp Val Ala Ala Pro Gly Ser Trp 
        195                 200                 205             


Ile Tyr Ser Thr Tyr Pro Thr Ser Thr Tyr Ala Ser Leu Ser Gly Thr 
    210                 215                 220                 


Ser Met Ala Thr Pro His Val Ala Gly Val Ala Gly Leu Leu Ala Ser 
225                 230                 235                 240 


Gln Gly Arg Ser Ala Ser Asn Ile Arg Ala Ala Ile Glu Asn Thr Ala 
                245                 250                 255     


Asp Lys Ile Ser Gly Thr Gly Thr Tyr Trp Ala Lys Gly Arg Val Asn 
            260                 265                 270         


Ala Tyr Lys Ala Val Gln Tyr 
        275                 


<210>  27
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  27
gttcaacata tgccagctt                                                    19


<210>  28
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  28
cgcacctgca acatga                                                       16


<210>  29
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial DNA Primer

<400>  29
acggtacatc gatggc                                                       16


