                               SEQUENCE LISTING

<110> PURDUE RESEARCH FOUNDATION
      ENDOCYTE, INC.
 
<120> METHODS AND COMPOSITIONS FOR CAR T CELL THERAPY

<130> 3220-264637

<140> PCT/US2017/026618
<141> 2017-04-07

<150> 62/323,971
<151> 2016-04-18

<150> 62/320,183
<151> 2016-04-08

<160> 4     

<170> PatentIn version 3.5

<210> 1
<211> 1554
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 1
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggatgtcg tgatgaccca gacccccctc agcctcccag tgtccctcgg tgaccaggct      120

tctattagtt gcagatccag ccagtccctc gtgcactcta acggtaatac ctacctgaga      180

tggtatctcc agaagcccgg acagagccct aaggtgctga tctacaaagt ctccaaccgg      240

gtgtctggag tccctgaccg cttctcaggg agcggttccg gcaccgactt caccctgaag      300

atcaaccggg tggaggccga agacctcggc gtctatttct gctctcagag tacacatgtg      360

ccctggacct tcggcggagg gaccaagctg gagatcaaaa gctccgcaga cgatgccaag      420

aaagatgccg ctaagaaaga cgatgctaag aaagacgatg caaagaaaga cggtggcgtg      480

aagctggatg aaaccggagg aggtctcgtc cagccaggag gagccatgaa gctgagttgc      540

gtgaccagcg gattcacctt tgggcactac tggatgaact gggtgcgaca gtccccagag      600

aaggggctcg aatgggtcgc tcagttcagg aacaaaccct acaattatga gacatactat      660

tcagacagcg tgaagggcag gtttactatc agtagagacg attccaaatc tagcgtgtac      720

ctgcagatga acaatctcag ggtcgaagat acaggcatct actattgcac aggggcatcc      780

tatggtatgg agtatctcgg tcaggggaca agcgtcacag tcagtttcgt gccggtcttc      840

ctgccagcga agcccaccac gacgccagcg ccgcgaccac caacaccggc gcccaccatc      900

gcgtcgcagc ccctgtccct gcgcccagag gcgtgccggc cagcggcggg gggcgcagtg      960

cacacgaggg ggctggactt cgcctgtgat atctacatct gggcgccctt ggccgggact     1020

tgtggggtcc ttctcctgtc actggttatc accctttact gcaaccacag gaaccgtttc     1080

tctgttgtta aacggggcag aaagaaactc ctgtatatat tcaaacaacc atttatgaga     1140

ccagtacaaa ctactcaaga ggaagatggc tgtagctgcc gatttccaga agaagaagaa     1200

ggaggatgtg aactgagagt gaagttcagc aggagcgcag acgcccccgc gtaccagcag     1260

ggccagaacc agctctataa cgagctcaat ctaggacgaa gagaggagta cgatgttttg     1320

gacaagagac gtggccggga ccctgagatg gggggaaagc cgagaaggaa gaaccctcag     1380

gaaggcctgt acaatgaact gcagaaagat aagatggcgg aggcctacag tgagattggg     1440

atgaaaggcg agcgccggag gggcaagggg cacgatggcc tttaccaggg tctcagtaca     1500

gccaccaagg acacctacga cgcccttcac atgcaggccc tgccccctcg ctaa           1554


<210> 2
<211> 517
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu 
            20                  25                  30          


Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln 
        35                  40                  45              


Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu Arg Trp Tyr Leu Gln 
    50                  55                  60                  


Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr Lys Val Ser Asn Arg 
65                  70                  75                  80  


Val Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                85                  90                  95      


Phe Thr Leu Lys Ile Asn Arg Val Glu Ala Glu Asp Leu Gly Val Tyr 
            100                 105                 110         


Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr 
        115                 120                 125             


Lys Leu Glu Ile Lys Ser Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala 
    130                 135                 140                 


Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Gly Gly Val 
145                 150                 155                 160 


Lys Leu Asp Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly Ala Met 
                165                 170                 175     


Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Gly His Tyr Trp Met 
            180                 185                 190         


Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Gln 
        195                 200                 205             


Phe Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp Ser Val 
    210                 215                 220                 


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr 
225                 230                 235                 240 


Leu Gln Met Asn Asn Leu Arg Val Glu Asp Thr Gly Ile Tyr Tyr Cys 
                245                 250                 255     


Thr Gly Ala Ser Tyr Gly Met Glu Tyr Leu Gly Gln Gly Thr Ser Val 
            260                 265                 270         


Thr Val Ser Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr 
        275                 280                 285             


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
    290                 295                 300                 


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
305                 310                 315                 320 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
                325                 330                 335     


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
            340                 345                 350         


Tyr Cys Asn His Arg Asn Arg Phe Ser Val Val Lys Arg Gly Arg Lys 
        355                 360                 365             


Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
    370                 375                 380                 


Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
385                 390                 395                 400 


Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
                405                 410                 415     


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
            420                 425                 430         


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
        435                 440                 445             


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
    450                 455                 460                 


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
465                 470                 475                 480 


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
                485                 490                 495     


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
            500                 505                 510         


Ala Leu Pro Pro Arg 
        515         


<210> 3
<211> 1560
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 3
gccaccatgg ccttaccagt gaccgccttg ctcctgccgc tggccttgct gctccacgcc       60

gccaggccgg atgtcgtgat gacccagacc cccctcagcc tcccagtgtc cctcggtgac      120

caggcttcta ttagttgcag atccagccag tccctcgtgc actctaacgg taatacctac      180

ctgagatggt atctccagaa gcccggacag agccctaagg tgctgatcta caaagtctcc      240

aaccgggtgt ctggagtccc tgaccgcttc tcagggagcg gttccggcac cgacttcacc      300

ctgaagatca accgggtgga ggccgaagac ctcggcgtct atttctgctc tcagagtaca      360

catgtgccct ggaccttcgg cggagggacc aagctggaga tcaaaagctc cgcagacgat      420

gccaagaaag atgccgctaa gaaagacgat gctaagaaag acgatgcaaa gaaagacggt      480

ggcgtgaagc tggatgaaac cggaggaggt ctcgtccagc caggaggagc catgaagctg      540

agttgcgtga ccagcggatt cacctttggg cactactgga tgaactgggt gcgacagtcc      600

ccagagaagg ggctcgaatg ggtcgctcag ttcaggaaca aaccctacaa ttatgagaca      660

tactattcag acagcgtgaa gggcaggttt actatcagta gagacgattc caaatctagc      720

gtgtacctgc agatgaacaa tctcagggtc gaagatacag gcatctacta ttgcacaggg      780

gcatcctatg gtatggagta tctcggtcag gggacaagcg tcacagtcag tttcgtgccg      840

gtcttcctgc cagcgaagcc caccacgacg ccagcgccgc gaccaccaac accggcgccc      900

accatcgcgt cgcagcccct gtccctgcgc ccagaggcgt gccggccagc ggcggggggc      960

gcagtgcaca cgagggggct ggacttcgcc tgtgatatct acatctgggc gcccttggcc     1020

gggacttgtg gggtccttct cctgtcactg gttatcaccc tttactgcaa ccacaggaac     1080

cgtttctctg ttgttaaacg gggcagaaag aaactcctgt atatattcaa acaaccattt     1140

atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa     1200

gaagaaggag gatgtgaact gagagtgaag ttcagcagga gcgcagacgc ccccgcgtac     1260

cagcagggcc agaaccagct ctataacgag ctcaatctag gacgaagaga ggagtacgat     1320

gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgag aaggaagaac     1380

cctcaggaag gcctgtacaa tgaactgcag aaagataaga tggcggaggc ctacagtgag     1440

attgggatga aaggcgagcg ccggaggggc aaggggcacg atggccttta ccagggtctc     1500

agtacagcca ccaaggacac ctacgacgcc cttcacatgc aggccctgcc ccctcgctaa     1560


<210> 4
<211> 519
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Ala Thr Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu 
1               5                   10                  15      


Leu Leu His Ala Ala Arg Pro Asp Val Val Met Thr Gln Thr Pro Leu 
            20                  25                  30          


Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser 
        35                  40                  45              


Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu Arg Trp Tyr 
    50                  55                  60                  


Leu Gln Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr Lys Val Ser 
65                  70                  75                  80  


Asn Arg Val Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly 
                85                  90                  95      


Thr Asp Phe Thr Leu Lys Ile Asn Arg Val Glu Ala Glu Asp Leu Gly 
            100                 105                 110         


Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly 
        115                 120                 125             


Gly Thr Lys Leu Glu Ile Lys Ser Ser Ala Asp Asp Ala Lys Lys Asp 
    130                 135                 140                 


Ala Ala Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Gly 
145                 150                 155                 160 


Gly Val Lys Leu Asp Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly 
                165                 170                 175     


Ala Met Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Gly His Tyr 
            180                 185                 190         


Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 
        195                 200                 205             


Ala Gln Phe Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp 
    210                 215                 220                 


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser 
225                 230                 235                 240 


Val Tyr Leu Gln Met Asn Asn Leu Arg Val Glu Asp Thr Gly Ile Tyr 
                245                 250                 255     


Tyr Cys Thr Gly Ala Ser Tyr Gly Met Glu Tyr Leu Gly Gln Gly Thr 
            260                 265                 270         


Ser Val Thr Val Ser Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr 
        275                 280                 285             


Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser 
    290                 295                 300                 


Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly 
305                 310                 315                 320 


Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp 
                325                 330                 335     


Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile 
            340                 345                 350         


Thr Leu Tyr Cys Asn His Arg Asn Arg Phe Ser Val Val Lys Arg Gly 
        355                 360                 365             


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
    370                 375                 380                 


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
385                 390                 395                 400 


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
                405                 410                 415     


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
            420                 425                 430         


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
        435                 440                 445             


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
    450                 455                 460                 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
465                 470                 475                 480 


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
                485                 490                 495     


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
            500                 505                 510         


Met Gln Ala Leu Pro Pro Arg 
        515                 


