                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Certo, Michael
       Leung, Wai-Hang
 
<120>  CHIMERIC ANTIGEN RECEPTOR T CELL COMPOSITIONS

<130>  BLBD-067/02WO

<150>  US 62/322,547
<151>  2016-04-14

<150>  US 62/319,703
<151>  2016-04-07

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  909
<212>  DNA
<213>  Ophiostoma novo-ulmi

<400>  1
atggcataca tgtcgcgcag agagtccatc aacccatgga ttctgactgg tttcgctgat       60

gccgaaggat ccttcttgct gagaatccga aacaataaca agagctccgt gggttactct      120

accgagttgg gctttcaaat cactctgcac aacaaggaca aatcgattct ggagaatatc      180

cagtcgactt ggaaggtcgg cgtgattgct aactcaggcg acaatgccgt cagtctgaaa      240

gttacgcgtt tcgaagattt gaaagtgatt atcgaccact tcgagaaata tccgctgatt      300

acccagaaat tgggcgatta caagttgttt aaacaggcat tcagcgtcat ggagaacaaa      360

gaacatctta aggagaatgg gattaaggag ctcgtacgaa tcaaagctaa gatgaattgg      420

ggtctcactg acgaattgaa aaaagcattt ccagagaaca ttagcaaaga gcgccccctt      480

atcaataaga acattccgaa tttcaaatgg ctggctggat tcacatctgg tgaaggctgc      540

ttctttgtga acttgatcaa gtccaaatct aagctgggtg tacaggttca attggtcttc      600

agcattactc agcacatcag agacaagaac ctgatgaatt cattgataac atacctaggc      660

tgtggttaca tcaaagagaa gaacaagtcc gagttcagtt ggctcgactt tgtggttacc      720

aaattcagcg atatcaacga caagatcatt ccggtattcc aggaaaatac tctgattggc      780

gtcaaactcg aggactttga agattggtgc aaggttgcca aattgatcga agagaagaaa      840

cacctgaccg aatccggttt ggatgagatt aagaaaatca agctgaacat gaacaaaggt      900

cgtgtcttc                                                              909


<210>  2
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  2

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  3
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  3
tgtctgccta ttcaccgatt tt                                                22


<210>  4
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  4
ctagcacagt tttgtctgtg at                                                22


<210>  5
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered I-OnuI variant

<400>  5

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
            20                  25                  30          


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Ser Gly Asp Arg Ala Val Met Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Asn Ala 
            180                 185                 190         


Lys Val Tyr Val Gly Leu Arg Phe Ser Ile Thr Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
    210                 215                 220                 


Arg Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Glu Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  6
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered I-OnuI variant

<400>  6

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
            20                  25                  30          


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Ser Gly Asp Arg Ala Val Met Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Asn Ala 
            180                 185                 190         


Lys Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
    210                 215                 220                 


Trp Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Glu Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  7
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered I-OnuI variant

<400>  7

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
            20                  25                  30          


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala 
                165                 170                 175     


Gly Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala 
            180                 185                 190         


Lys Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
    210                 215                 220                 


Trp Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  8
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized polypeptide of anti-CD19 CAR

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg 
                325                 330                 335     


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
            340                 345                 350         


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
        355                 360                 365             


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            420                 425                 430         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        435                 440                 445             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    450                 455                 460                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
465                 470                 475                 480 


Gln Ala Leu Pro Pro Arg 
                485     


<210>  9
<211>  8324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized plasmid pBW1021

<400>  9
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca     3900

gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg     3960

cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag     4020

ggagtggcca actccatcac taggggttcc tgcggccgct acggctgggg cttagacgca     4080

ggtgttctga tttatagttc aaaacctcta tcaatgagag agcaatctcc tggtaatgtg     4140

atagatttcc caacttaatg ccaacatacc ataaacctcc cattctgcta atgcccagcc     4200

taagttgggg agaccactcc agattccaag atgtacagtt tgctttgctg ggcctttttc     4260

ccatgcctgc ctttactctg ccagagttat attgctgggg ttttgaagaa gatcctatta     4320

aataaaagaa taagcagtat tattaagtag ccctgcattt caggtttcct tgagtggcag     4380

gccaggcctg gcgtgaacgt tcactgaaat catggcctct tggccaagat tgatagcttg     4440

tgcctgtccc tgagtcccag tccatcacga gcagctggtt tctaagatgc tatttcccgt     4500

ataaagcatg agaccgtgac ttgccagccc cacagagccc cgcccttgtc catcactggc     4560

atctggactc cagcctgggt tggggcaaag agggaaatga gatcatgtcc taaccctgat     4620

cctcttgtcc cacagatatc cagaaccctg accctgccgt gtaccagctg agagactcta     4680

aatccagtga caagtctgtc tgcctatacg cgtgatccat cgattagtcc aatttgttaa     4740

agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc     4800

tatagagtac gagccataga tagaataaaa gattttattt agtctccaga aaaagggggg     4860

aatgaaagac cccacctgta ggtttggcaa gctaggatca aggttaggaa cagagagaca     4920

gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     4980

agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc     5040

cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta     5100

gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt     5160

gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa     5220

taaaagagcc cacaacccct cactcggcgc gacgcgtcat agccaccatg gccttaccag     5280

tgaccgcctt gctcctgccg ctggccttgc tgctccacgc cgccaggccg gacatccaga     5340

tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc atcagttgca     5400

gggcaagtca ggacattagt aaatatttaa attggtatca gcagaaacca gatggaactg     5460

ttaaactcct gatctaccat acatcaagat tacactcagg agtcccatca aggttcagtg     5520

gcagtgggtc tggaacagat tattctctca ccattagcaa cctggagcaa gaagatattg     5580

ccacttactt ttgccaacag ggtaatacgc ttccgtacac gttcggaggg gggaccaagc     5640

tggagatcac aggtggcggt ggctccggcg gtggtgggtc tggtggcggc ggaagcgagg     5700

tgaaactgca ggagtcagga cctggcctgg tggcgccctc acagagcctg tccgtcacat     5760

gcactgtctc aggggtctca ttacccgact atggtgtaag ctggattcgc cagcctccac     5820

gaaagggtct ggagtggctg ggagtaatat ggggtagtga aaccacatac tataattcag     5880

ctctcaaatc cagactgacc atcatcaagg acaactccaa gagccaagtt ttcttaaaaa     5940

tgaacagtct gcaaactgat gacacagcca tttactactg tgccaaacat tattactacg     6000

gtggtagcta tgctatggac tactggggtc aaggaacctc ggtcaccgtc tcctcaacca     6060

cgacgccagc gccgcgacca ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc     6120

tgcgcccaga ggcgtgccgg ccagcggcgg ggggcgcagt gcacacgagg gggctggact     6180

tcgcctgtga tatctacatc tgggcgccct tggccgggac ttgtggggtc cttctcctgt     6240

cactggtgat caccctttac tgcaaacggg gcagaaagaa actcctgtat atattcaaac     6300

aaccatttat gagaccagta caaactactc aagaggaaga tggctgtagc tgccgatttc     6360

cagaagaaga agaaggagga tgtgaactga gagtgaagtt cagcaggagc gcagacgccc     6420

ccgcgtacca gcagggccag aaccagctct ataacgagct caatctagga cgaagagagg     6480

agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga aagccgagaa     6540

ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg gcggaggcct     6600

acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat ggcctttacc     6660

agggtctcag tacagccacc aaggacacct acgacgccct tcacatgcag gccctgcccc     6720

ctcgctaagc ggccgcgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc     6780

attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt     6840

cagggggaga tgtgggaggt tttttaaagc tcaccggttt tgattctcaa acaaatgtgt     6900

cacaaagtaa ggattctgat gtgtatatca cagacaaaac tgtgctagac atgaggtcta     6960

tggacttcaa gagcaacagt gctgtggcct ggagcaacaa atctgacttt gcatgtgcaa     7020

acgccttcaa caacagcatt attccagaag acaccttctt ccccagccca ggtaagggca     7080

gctttggtgc cttcgcaggc tgtttccttg cttcaggaat ggccaggttc tgcccagagc     7140

tctggtcaat gatgtctaaa actcctctga ttggtggtct cggccttatc cattgccacc     7200

aaaaccctct ttttactaag aaacagtgag ccttgttctg gcagtccaga gaatgacacg     7260

ggaaaaaagc agatgaagag aaggtggcag gagagggcac gtggcccagc ctcagtctct     7320

ccaactgagt tcctgcctgc ctgcctttgc tcagactgtt tgccccttac tgctcttcta     7380

ggcctcattc taagcccctt ctccaagttg cctctcctta tttctccctg tctgccaaaa     7440

aatctttccc agctcactaa gtcagtctca cgcagtcact cattaaccca ccaatcactg     7500

attgtgccgg cacatgaatg caccaggtag cggccgcagg aacccctagt gatggagttg     7560

gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga     7620

cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcaggaa     7680

gctgtaagct tgtcgagaag tactagagga tcataatcag ccataccaca tttgtagagg     7740

ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg     7800

caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca     7860

tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac     7920

tcatcaatgt atcttatcat gtctggatct gatcactgat atcgcctagg agatccgaac     7980

cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac     8040

gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc     8100

catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc atttttcttc     8160

ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc atcttcggct     8220

actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt aatgtttgta     8280

attgactgaa tatcaacgct tatttgcagc ctgaatggcg aatg                      8324


<210>  10
<211>  39
<212>  DNA
<213>  Homo sapiens

<400>  10
aaatccagtg acaagtctgt ctgcctattc accgatttt                              39


<210>  11
<211>  887
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized polypeptide - illustrative example of a TCRalpha 
       I-OnuI megaTAL

<400>  11

Met Gly Ser Cys Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Pro Pro 
1               5                   10                  15      


Lys Lys Lys Arg Lys Val Val Asp Leu Arg Thr Leu Gly Tyr Ser Gln 
            20                  25                  30          


Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val Ala Gln 
        35                  40                  45              


His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His Ile Val 
    50                  55                  60                  


Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val Thr Tyr 
65                  70                  75                  80  


Gln His Ile Ile Thr Ala Leu Pro Glu Ala Thr His Glu Asp Ile Val 
                85                  90                  95      


Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala Leu Leu 
            100                 105                 110         


Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp Thr Gly 
        115                 120                 125             


Gln Leu Val Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Met Glu Ala 
    130                 135                 140                 


Val His Ala Ser Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn Leu Thr 
145                 150                 155                 160 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
                165                 170                 175     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            180                 185                 190         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
        195                 200                 205             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    210                 215                 220                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly 
225                 230                 235                 240 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                245                 250                 255     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            260                 265                 270         


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        275                 280                 285             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    290                 295                 300                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
305                 310                 315                 320 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                325                 330                 335     


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            340                 345                 350         


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
        355                 360                 365             


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
    370                 375                 380                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
385                 390                 395                 400 


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
                405                 410                 415     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
            420                 425                 430         


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala 
        435                 440                 445             


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
    450                 455                 460                 


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
465                 470                 475                 480 


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
                485                 490                 495     


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly 
            500                 505                 510         


Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp 
        515                 520                 525             


Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys 
    530                 535                 540                 


Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys Lys Gly Leu Pro His 
545                 550                 555                 560 


Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg Ile Gly Glu Arg Thr 
                565                 570                 575     


Ser His Arg Val Ala Ile Ser Arg Val Gly Gly Ser Ser Arg Arg Glu 
            580                 585                 590         


Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
        595                 600                 605             


Phe Ile Leu Asp Ile Arg Asn Arg Asn Asn Glu Ser Asn Arg Tyr Arg 
    610                 615                 620                 


Thr Ser Leu Arg Phe Gln Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
625                 630                 635                 640 


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Thr Asn Ser 
                645                 650                 655     


Gly Asp Arg Ala Val Met Leu Arg Val Thr Arg Phe Glu Asp Leu Lys 
            660                 665                 670         


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
        675                 680                 685             


Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn Lys 
    690                 695                 700                 


Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
705                 710                 715                 720 


Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala Phe Pro Glu 
                725                 730                 735     


Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Phe 
            740                 745                 750         


Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Tyr Phe Gly Val Asn 
        755                 760                 765             


Leu Lys Lys Val Lys Gly Asn Ala Lys Val Tyr Val Gly Leu Arg Phe 
    770                 775                 780                 


Ser Ile Ser Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile 
785                 790                 795                 800 


Thr Tyr Leu Gly Cys Gly Ser Ile Trp Glu Lys Asn Lys Ser Glu Phe 
                805                 810                 815     


Ser Trp Leu Glu Phe Val Val Thr Lys Phe Ser Asp Ile Asn Asp Lys 
            820                 825                 830         


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
        835                 840                 845             


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
    850                 855                 860                 


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
865                 870                 875                 880 


Met Asn Lys Gly Arg Val Phe 
                885         


<210>  12
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized polypeptide - illustrative example of an anti-BCMA 
       CAR

<400>  12

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Val Leu Thr Gln Ser Pro Pro Ser Leu 
            20                  25                  30          


Ala Met Ser Leu Gly Lys Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu 
        35                  40                  45              


Ser Val Thr Ile Leu Gly Ser His Leu Ile His Trp Tyr Gln Gln Lys 
    50                  55                  60                  


Pro Gly Gln Pro Pro Thr Leu Leu Ile Gln Leu Ala Ser Asn Val Gln 
65                  70                  75                  80  


Thr Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe 
                85                  90                  95      


Thr Leu Thr Ile Asp Pro Val Glu Glu Asp Asp Val Ala Val Tyr Tyr 
            100                 105                 110         


Cys Leu Gln Ser Arg Thr Ile Pro Arg Thr Phe Gly Gly Gly Thr Lys 
        115                 120                 125             


Leu Glu Ile Lys Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly 
    130                 135                 140                 


Glu Gly Ser Thr Lys Gly Gln Ile Gln Leu Val Gln Ser Gly Pro Glu 
145                 150                 155                 160 


Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly 
                165                 170                 175     


Tyr Thr Phe Thr Asp Tyr Ser Ile Asn Trp Val Lys Arg Ala Pro Gly 
            180                 185                 190         


Lys Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Arg Glu Pro 
        195                 200                 205             


Ala Tyr Ala Tyr Asp Phe Arg Gly Arg Phe Ala Phe Ser Leu Glu Thr 
    210                 215                 220                 


Ser Ala Ser Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Tyr Glu Asp 
225                 230                 235                 240 


Thr Ala Thr Tyr Phe Cys Ala Leu Asp Tyr Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ala Ala Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490             


<210>  13
<211>  8619
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthesized polynucleotide - plasmid pBW400

<400>  13
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca     3900

gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg     3960

cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag     4020

ggagtggcca actccatcac taggggttcc tgcggccgct acggctgggg cttagacgca     4080

ggtgttctga tttatagttc aaaacctcta tcaatgagag agcaatctcc tggtaatgtg     4140

atagatttcc caacttaatg ccaacatacc ataaacctcc cattctgcta atgcccagcc     4200

taagttgggg agaccactcc agattccaag atgtacagtt tgctttgctg ggcctttttc     4260

ccatgcctgc ctttactctg ccagagttat attgctgggg ttttgaagaa gatcctatta     4320

aataaaagaa taagcagtat tattaagtag ccctgcattt caggtttcct tgagtggcag     4380

gccaggcctg gcgtgaacgt tcactgaaat catggcctct tggccaagat tgatagcttg     4440

tgcctgtccc tgagtcccag tccatcacga gcagctggtt tctaagatgc tatttcccgt     4500

ataaagcatg agaccgtgac ttgccagccc cacagagccc cgcccttgtc catcactggc     4560

atctggactc cagcctgggt tggggcaaag agggaaatga gatcatgtcc taaccctgat     4620

cctcttgtcc cacagatatc cagaaccctg accctgccgt gtaccagctg agagactcta     4680

aatccagtga caagtctgtc tgcctatacg cgtgatccat cgattagtcc aatttgttaa     4740

agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc     4800

tatagagtac gagccataga tagaataaaa gattttattt agtctccaga aaaagggggg     4860

aatgaaagac cccacctgta ggtttggcaa gctaggatca aggttaggaa cagagagaca     4920

gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     4980

agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc     5040

cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta     5100

gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt     5160

gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa     5220

taaaagagcc cacaacccct cactcggcgc gacgcgtcat agccaccatg gccttaccag     5280

tgaccgcctt gctcctgccg ctggccttgc tgctccacgc cgccaggccg gacatccaga     5340

tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc atcagttgca     5400

gggcaagtca ggacattagt aaatatttaa attggtatca gcagaaacca gatggaactg     5460

ttaaactcct gatctaccat acatcaagat tacactcagg agtcccatca aggttcagtg     5520

gcagtgggtc tggaacagat tattctctca ccattagcaa cctggagcaa gaagatattg     5580

ccacttactt ttgccaacag ggtaatacgc ttccgtacac gttcggaggg gggaccaagc     5640

tggagatcac aggtggcggt ggctccggcg gtggtgggtc tggtggcggc ggaagcgagg     5700

tgaaactgca ggagtcagga cctggcctgg tggcgccctc acagagcctg tccgtcacat     5760

gcactgtctc aggggtctca ttacccgact atggtgtaag ctggattcgc cagcctccac     5820

gaaagggtct ggagtggctg ggagtaatat ggggtagtga aaccacatac tataattcag     5880

ctctcaaatc cagactgacc atcatcaagg acaactccaa gagccaagtt ttcttaaaaa     5940

tgaacagtct gcaaactgat gacacagcca tttactactg tgccaaacat tattactacg     6000

gtggtagcta tgctatggac tactggggtc aaggaacctc ggtcaccgtc tcctcaacca     6060

cgacgccagc gccgcgacca ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc     6120

tgcgcccaga ggcgtgccgg ccagcggcgg ggggcgcagt gcacacgagg gggctggact     6180

tcgcctgtga tatctacatc tgggcgccct tggccgggac ttgtggggtc cttctcctgt     6240

cactggtgat caccctttac tgcaaacggg gcagaaagaa actcctgtat atattcaaac     6300

aaccatttat gagaccagta caaactactc aagaggaaga tggctgtagc tgccgatttc     6360

cagaagaaga agaaggagga tgtgaactga gagtgaagtt cagcaggagc gcagacgccc     6420

ccgcgtacca gcagggccag aaccagctct ataacgagct caatctagga cgaagagagg     6480

agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga aagccgagaa     6540

ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg gcggaggcct     6600

acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat ggcctttacc     6660

agggtctcag tacagccacc aaggacacct acgacgccct tcacatgcag gccctgcccc     6720

ctcgctaagc ggccgctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta     6780

ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc     6840

atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc     6900

ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt     6960

tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg     7020

taaccattct agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat     7080

aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg     7140

ggagatgtgg gaggtttttt aaagctcacc ggttttgatt ctcaaacaaa tgtgtcacaa     7200

agtaaggatt ctgatgtgta tatcacagac aaaactgtgc tagacatgag gtctatggac     7260

ttcaagagca acagtgctgt ggcctggagc aacaaatctg actttgcatg tgcaaacgcc     7320

ttcaacaaca gcattattcc agaagacacc ttcttcccca gcccaggtaa gggcagcttt     7380

ggtgccttcg caggctgttt ccttgcttca ggaatggcca ggttctgccc agagctctgg     7440

tcaatgatgt ctaaaactcc tctgattggt ggtctcggcc ttatccattg ccaccaaaac     7500

cctcttttta ctaagaaaca gtgagccttg ttctggcagt ccagagaatg acacgggaaa     7560

aaagcagatg aagagaaggt ggcaggagag ggcacgtggc ccagcctcag tctctccaac     7620

tgagttcctg cctgcctgcc tttgctcaga ctgtttgccc cttactgctc ttctaggcct     7680

cattctaagc cccttctcca agttgcctct ccttatttct ccctgtctgc caaaaaatct     7740

ttcccagctc actaagtcag tctcacgcag tcactcatta acccaccaat cactgattgt     7800

gccggcacat gaatgcacca ggtagcggcc gcaggaaccc ctagtgatgg agttggccac     7860

tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc     7920

gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggaagctgt     7980

aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta     8040

cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt     8100

gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca     8160

aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc     8220

aatgtatctt atcatgtctg gatctgatca ctgatatcgc ctaggagatc cgaaccagat     8280

aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc     8340

tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt     8400

ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt     8460

atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt     8520

ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga     8580

ctgaatatca acgcttattt gcagcctgaa tggcgaatg                            8619


<210>  14
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  14

Gly Gly Gly 
1           


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  15

Asp Gly Gly Gly Ser 
1               5   


<210>  16
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  16

Thr Gly Glu Lys Pro 
1               5   


<210>  17
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  17

Gly Gly Arg Arg 
1               


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  18

Gly Gly Gly Gly Ser 
1               5   


<210>  19
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  19

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  20
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  20

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  21

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  22
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  22

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  23

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  24
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  24

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  25

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  26

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  27

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  28
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  28

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  29
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  29

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  30
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  30

Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10                  


<210>  31
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  31

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  32
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  32

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  33
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  33

Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10              


<210>  34
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  34

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  35
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  35

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  36
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  36

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  37
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  37

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  38
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  38

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  39
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  39

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  40
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  40

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  41
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  41

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  42
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  42

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  43
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  43

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  44
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  44

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  45
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  45

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  46
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  46

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  47
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  47

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  48
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  48

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  49
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  49

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  50
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus Kozak sequence

<400>  50
gccrccatgg                                                              10


