SEQUENCE LISTING

<110> CSIR
 
<120> A nucleotide sequence encoding epoxide hydrolase 2 and recombinant constructs comprising the same

<150>201611011975

<151> 05-03-2016

<160>  26

<210> 1
<211> 957
<212> cDNA
<213> Artificial sequence 
<220>
<223> cDNA 

<400> 1

atggaagata tacagcacag aattgtgaat gtcaatggct taaacatgca cgtggcagag      60
aaaggcgaag gtccagtcat tctcttcatt cacggttttc ccgaactgtg gtactcctgg     120
cggcatcaga tcatcgcctt ggcttccctc ggctaccgag ccattgctcc ggatctacgt     180
ggcttcggtg atactgacgc gccgccgtct gtctcgagtt acacgtgttt ccacgtggtg     240
ggggacctca ttggacttct cgacgtcgtt gcctctgatc gggataaggt tttcgtggtg     300
ggccatgatt ggggtgctct tattgcttgg tacttgtgct tgtttagacc ggataaggtc     360
aaagctttgg tcaacttgag tgttgcgttt aatccctgga accccaagag gaagccactt     420
gagggtttga aagctgttta tggtgacgat tattacatga tcagatttca ggagcctggt     480
gagatagaag ctgagtttgc ccagattggt actgagacag ttgtcaagga attctttaca     540
tacaggacac ctggtcctct ttttctccct acaggtaaag gatttggaca tcccccaaat     600
gctgaaattg tcttaccctc ttggctatca gaggatgatg ttaaaaacta caccagcaaa     660
tttgagaaag gctttacagg aggagtgaac tattaccgta atataaacgt gaactgggaa     720
cttacagctc cttgggccgg gagtcaaata aaggttcctg ttaagttcat cgtgggtgac     780
ctggacctga catattatat gccaggagtc aaggattaca tacacaaagg tgggttcaag     840
cgagatgtgc cattattgga agaagtgatt gtaatggaag gtgtaggtca cttcattaat     900
ggagaaaagg ctgatgcaat cagtgagcac atatacaact tttttcagaa gttctga        957

<210> 2
<211> 1875
<212> DNA
<213> Mangifera indica
<220>
<223> Nucleotide sequence encoding epoxide hydrolase 2

<400> 2

atggaagata tacagcacag aattgtgaat gtcaatggct taaacatgca cgtggcagag      60
aaaggcgaag gtccagtcat tctcttcatt cacggttttc ccgaactgtg gtactcctgg     120
cggcatcaga tcatcgcctt ggcttccctc ggctaccgag ccattgctcc ggatctacgt     180
ggcttcggtg atactgacgc gccgccgtct gtctcgagtt acacgtgttt ccacgtggtg     240
ggggacctca ttggacttct cgacgtcgtt gcctctgatc gggataaggt tttcgtggtg     300
ggccatgatt ggggtgctct tattgcttgg tacttgtgct tgtttagacc ggataaggtc     360
aaagctttgg tcaacttgag tgttgcgttt aatccctgga accccaagag gaagccactt     420
gagggtttga aagctgttta tggtgacgat tattacatga tcagatttca ggttcatttt     480
tcaacttatt ttagttttgt aattgttttt tctaactgct ttgggggttt tgcttctgct     540
gctaattaag ttatcaatga atgattgatt tgctgcaaag ttttgacctt tgatttctta     600
tttgctttga tccttgaatg tttcatcaga tctttactga aacaacatga attttagcga     660
acctggaaag taggaagctc tgtatagata ttgctcgttg gtttagaatt tctactatag     720
tgctaaagct agcttttgaa atggagagtt taccgccacc tctacagcgg tgatggctca     780
ctgttagcct tgggtaaaca ctgtaaaagt tagttattga aattagaaaa tctaaaacta     840
tataaaccct aaatcaaaga ccatattttt tgatgtagaa atcaaatctc gtagcataca     900
cttggagtga taacatttca gtctgtttga agtagggttc tttgatcaca tcatcttgca     960
catgtcatag catctttaat ttgagcatgt gaaagataaa gttagaagtt tacatttatt    1020
aatcatagat tcagttgatc ttgttgccca tgaacttaaa ttttttattt gttccaaagt    1080
tttaaggcag ttctactgtc attccataac atgccagagc attttgccct gtagctggag    1140
gttctacatc aattcattta ctgatgaata tcaatcatag agcacaattt cgcattttga    1200
taaaatcaaa atagctcacc taatgaaccg attgcttgac gggattgtag gagcctggtg    1260
agatagaagc tgagtttgcc cagattggta ctgagacagt tgtcaaggaa ttctttacat    1320
acaggacacc tggtcctctt tttctcccta caggtaaagg atttggacat cccccaaatg    1380
ctgaaattgt cttaccctct tggctatcag aggatgatgt taaaaactac accagcaaat    1440
ttgagaaagg ctttacagga ggagtgaact attaccgtaa tataaacgtg tatgtctcct    1500
tccaagtcat atgagcagct atatctgttt tgcttgatcc aatctggcat gaatttgatt    1560
atctagtggt gatcttttac tcaattatat ggcattgtca ctcatgttta ctctcctttt    1620
tgctgcagga actgggaact tacagctcct tgggccggga gtcaaataaa ggttcctgtt    1680
aagttcatcg tgggtgacct ggacctgaca tattatatgc caggaaggaa ggattacata    1740
cacaaaggtg ggttcaagcg agatgtgcca ttattggaag aagtgattgt aatggaaggt    1800
gtaggtcact tcattaatgg agaaaaggct gatgcaatca gtgagcacat atacaacttt    1860
tttcagaagt tctga                                                     1875

<210> 3
<211> 318
<212> PRT
<213> Mangifera indica
<220>
<223> PRT sequence of epoxide hydrolase 

<400> 3
Met Glu Asp Ile Gln His Arg Ile Val Asn Val Asn Gly Leu Asn Met 
His Val Ala Glu Lys Gly Glu Gly Pro Val Ile Leu Phe Ile His Gly 
Phe Pro Glu Leu Trp Tyr Ser Trp Arg His Gln Ile Ile Ala Leu Ala
Ser Leu Gly Tyr Arg Ala Ile Ala Pro Asp Leu Arg Gly Phe Gly Asp
Thr Asp Ala Pro Pro Ser Val Ser Ser Tyr Thr Cys Phe His Val Val
Gly Asp Leu Ile Gly Leu Leu Asp Val Val Ala Ser Asp Arg Asp Lys
Val Phe Val Val Gly His Asp Trp Gly Ala Leu Ile Ala Trp Tyr Leu
Cys Leu Phe Arg Pro Asp Lys Val Lys Ala Leu Val Asn Leu Ser Val
Ala Phe Asn Pro Trp Asn Pro Lys Arg Lys Pro Leu Glu Gly Leu Lys 
Ala Val Tyr Gly Asp Asp Tyr Tyr Met Ile Arg Phe Gln Glu Pro Gly
Glu Ile Glu Ala Glu Phe Ala Gln Ile Gly Thr Glu Thr Val Val Lys 
Glu Phe Phe Thr Tyr Arg Thr Pro Gly Pro Leu Phe Leu Pro Thr Gly 
Lys Gly Phe Gly His Pro Pro Asn Ala Glu Ile Val Leu Pro Ser Trp
Leu Ser Glu Asp Asp Val Lys Asn Tyr Thr Ser Lys Phe Glu Lys Gly
Phe Thr Gly Gly Val Asn Tyr Tyr Arg Asn Ile Asn Val Asn Trp Glu
Leu Thr Ala Pro Trp Ala Gly Ser Gln Ile Lys Val Pro Val Lys Phe 
Ile Val Gly Asp Leu Asp Leu Thr Tyr Tyr Met Pro Gly Val Lys Asp
Tyr Ile His Lys Gly Gly Phe Lys Arg Asp Val Pro Leu Leu Glu Glu
Val Ile Val Met Glu Gly Val Gly His Phe Ile Asn Gly Glu Lys Ala 
Asp Ala Ile Ser Glu His Ile Tyr Asn Phe Phe Gln Lys Phe

<210> 4
<211> 708
<212> cDNA
<213> Artificial sequence
<220>
<223> cDNA sequence encoding peroxygenase

<400> 4
atggacgggg atgcaatggc aaccgaggct ccttatgcac cagtcactta tgagagaaga       60
gtccccactg acttggatga caaaattccc aagccttata tgccaagagc actggaagct      120
ccagatccga gccatccaaa tggaacacaa gggcacaata atcacaacct gagtgtgctt      180
cagcagcatg tagccttttt tgaccaagat gacaatggga tcatttaccc ttgggagact      240
tatacaggac taagagcaat tggtttcaac atgatagttt ctgtgatatt ggcctttgtc      300
atcaacttgg cactgagtta tcctactctc cccgggtgga tcccatctcc tttcttaccc      360
atttacatac acaacataca caaagcaaag catggcagtg actcaggaac atatgacacg      420
gaaggaaggt acatgcctgc aaacctggaa ttcatattta gcaaatattc tcatacagtg      480
cctggtaagt taacaatagg agagctttgg gacatgactg agggaaaccg tgtagcaatg      540
gacttctttg gctggattgc aagtaaaggg gaatggcttg ttttatacat tttggcgagg      600
gacgaggaag gtttcctatc aaaagaagct gtgagacgct gttacgatgg tagtttgttt      660
gagtactgtg caaaactgaa tgcaggcgct gcagctaaga tgatttaa                   708


<210> 5
<211> 1515
<212> DNA
<213> Mangifera indica
<220>
<223> DNA sequence encoding peroxygenase

<400> 5

atggacgggg atgcaatggc aaccgaggct ccttatgcac cagtcactta tgagagaaga      60
gtccccactg acttggatga caaaattccc aagccttgta tgctttttcg cttccatttc     120
cactgctcta tttattattt atttttaatc ataaattgta acatatctag gcaaatccta     180
tttggacaaa atataggaga gatctcttat gttttgtgta tatatgaata ttccataccg     240
ttgaaaatgt taaaaaagat tgtttatctt aagttcttta aaaaagactg attattatgg     300
atatcttgga aaaaaaaaaa agacaaacct tattgtctta tacatgtcaa gaagatatgt     360
tttatatgta tgaacatctc atatgaatga taaaatggtt gaataatgtt ctaaattata     420
gagatgtctg atagactata tttaaagtag ataatgtcat aatagtggac taagttatta     480
aactagaata ttacataaat caaccattat gacagaatag taggagaaaa aaaagataaa     540
tcttattgtt aagttggttt ctagattgta aatttgtgag aaaattaagc tgtaaatttt     600
attcgatctg tctgatttat gttcaactta gatatgccaa gagcactgga agctccagat     660
ccgagccatc caaatggaac acaagggcac aataatcaca acctgagtgt gcttcagcag     720
catgtagcct tttttgacca agatgacaat gggatcattt acccttggga gacttataca     780
ggactaagag caattggttt caacatgata gtttctgtga tattggcctt tgtcatcaac     840
ttggcactga gttatcctac tctccccggg tggatcccat ctcctttctt acccatttac     900
atacacaaca tacacaaagc aaagcatggc agtgactcag gaacatatga cacggaagga     960
aggtacatgc ctgcaaacct ggaattcata tttagcaaat attctcatac agtgcctggt    1020
aagttaacaa taggagagct ttgggacatg actgagggaa accgtgtagc aatggacttc    1080
tttggctggt aagttgttaa tcaatgttgt atagctagtt tcagtagttg gatcactatg    1140
aaaatatggg ctgtgaaatt aaaacacctc ttaacttcac aatctattta agtaatcttg    1200
aatattttat atatatatct aacatctttc ccatattttg taaatgtaaa atttgtttaa    1260
aactattaca aacgcaccca tttcaatgaa ttgaactgtc atttttaact taaaagaaat    1320
gcactgaata agagtaaggg ttggttgcat atggattgca ggattgcaag taaaggggaa    1380
tggcttgttt tatacatttt ggcgagggac gaggaaggtt tcctatcaaa agaagctgtg    1440
agacgctgtt acgatggtag tttgtttgag tactgtgcaa aactgaatgc aggcgctgca    1500
gctaagatga tttaa                                                     1515


<210> 6
<211> 235
<212> PRT
<213> Mangifera indica
<220>
<223> polypeptide sequence of peroxygenase

<400> 6
Met Asp Gly Asp Ala Met Ala Thr Glu Ala Pro Tyr Ala Pro Val Thr
Tyr Glu Arg Arg Val Pro Thr Asp Leu Asp Asp Lys Ile Pro Lys Pro
Tyr Met Pro Arg Ala Leu Glu Ala Pro Asp Pro Ser His Pro Asn Gly
Thr Gln Gly His Asn Asn His Asn Leu Ser Val Leu Gln Gln His Val
Ala Phe Phe Asp Gln Asp Asp Asn Gly Ile Ile Tyr Pro Trp Glu Thr
Tyr Thr Gly Leu Arg Ala Ile Gly Phe Asn Met Ile Val Ser Val Ile
Leu Ala Phe Val Ile Asn Leu Ala Leu Ser Tyr Pro Thr Leu Pro Gly
Trp Ile Pro Ser Pro Phe Leu Pro Ile Tyr Ile His Asn Ile His Lys
Ala Lys His Gly Ser Asp Ser Gly Thr Tyr Asp Thr Glu Gly Arg Tyr 
Met Pro Ala Asn Leu Glu Phe Ile Phe Ser Lys Tyr Ser His Thr Val
Pro Gly Lys Leu Thr Ile Gly Glu Leu Trp Asp Met Thr Glu Gly Asn  
Arg Val Ala Met Asp Phe Phe Gly Trp Ile Ala Ser Lys Gly Glu Trp
Leu Val Leu Tyr Ile Leu Ala Arg Asp Glu Glu Gly Phe Leu Ser Lys
Glu Ala Val Arg Arg Cys Tyr Asp Gly Ser Leu Phe Glu Tyr Cys Ala
Lys Leu Asn Ala Gly Ala Ala Ala Lys Met Ile

<210> 7
<211> 17
<212> DNA
<213> Artificial sequence 
<220>
<223> EH DeF1primer 

<400> 7
ctytggtayt cvtggcg


<210> 8
<211> 14
<212> DNA
<213> Artificial sequence 
<220>
<223> EH DeR4 primer 

<400> 8
cchryccatg ghsc 

<210> 9
<211> 22
<212> DNA
<213> Artificial sequence 
<220>
<223> EHRCF2 primer 

<400> 9

gtggcttcgg tgatactgac gc
 
<210> 10
<211> 22
<212> DNA
<213> Artificial sequence 
<220>
<223> EHRCR1 primer 
<400> 10

cctgatcaga ggcaacgacg tc

<210> 11
<211> 27
<212> DNA
<213> Artificial sequence 

<220>
<223> EHtrF1 primer 

<400> 11

atggaagata tacagcacag aattgtg


<210> 12
<211> 28
<212> DNA
<213> Artificial sequence 

<220>
<223> EHtrR1 primer 
<400> 12

tcagaacttc tgaaaaaagt tgtatatg

<210> 13
<211> 24
<212> DNA
<213> Artificial sequence 
<220>
<223> EHTOPO_F1 primer

<400> 13
atggaagata tacagcacag aatt

<210> 14
<211> 26
<212> DNA
<213> Artificial sequence 
<220>
<223> EHTOPO_R1 primer
<400> 14

gaacttctg aaaaaagttg tatatg

<210> 15
<211> 39
<212> DNA
<213> Artificial sequence 
<220>
<223> EHpBI121F1 primer

<400> 15

aaaaaaggat ccatggaaga tatacagcac agaattgtg


<210> 16
<211> 43
<212> DNA
<213> Artificial sequence 
<220>
<223> EHpBI121R1 primer

<400> 16

aaaaaaggat cctcagaact tctgaaaaaa gttgtatatg tgc 


<210> 17
<211> 25
<212> DNA
<213> Artificial sequence 
<220>
<223> EHRTF4 primer

<400> 17

ccttgggccg ggagtcaaat aaagg


<210> 18
<211> 25
<212> DNA
<213> Artificial sequence 
<220>
<223> EHRTR4 primer

<400> 18

aatggcacat ctcgcttgaa cccac


<210> 19
<211> 22
<212> DNA
<213> Artificial sequence 
<220>
<223> PGX_De_F primer

<400> 19

mwgagygtbc tkcarcagca tg

<210> 20
<211> 19
<212> DNA
<213> Artificial sequence 
<220>
<223> PGX_De_R primer

<400> 20

amtcraacaa rctmccatc


<210> 21
<211> 23
<212> DNA
<213> Artificial sequence 
<220>
<223> PGX_RC_F primer

<400> 21

gggatcattt acccttggga gac


<210> 22
<211> 22
<212> DNA
<213> Artificial sequence 
<220>
<223> PGX_RC_R primer

<400> 22

cccctttact tgcaatccag cc


<210> 23
<211> 24
<212> DNA
<213> Artificial sequence 
<220>
<223> MiPGX_Tr_F primer

<400> 23

atggacgggg atgcaatggc aacc 


<210> 24
<211> 27
<212> DNA
<213> Artificial sequence 
<220>
<223> MiPGX_Tr_R primer

<400> 24

ttaaatcatc ttagctgcag cgcctgc


<210> 25
<211> 25
<212> DNA
<213> Artificial sequence 
<220>
<223> MiPGX1_RT_F1 primer

<400> 25

aaggaaggta catgcctgca aacct
 

<210> 26
<211> 25
<212> DNA
<213> Artificial sequence 
<220>
<223> MiPGX1_RT_R1 primer

<400> 26

cggtttccct cagtcatgtc ccaaa 
 


