                         SEQUENCE LISTING

<110>  Council of Scientific and Industrial Research
       Rao, Alka
       Nagar, Rupa
 
<120>  A method for O- and S- glycosylation of peptides or polypeptides 
       using a multifunctional glycosyltransferase

<130>  xxx

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  455
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  C-term 6X His tagged recombinant Glycosyltransferase protein

<400>  1

Met Gly Tyr Ser Glu Asn Phe Ile Ala Asn Asp Trp Phe Asn Val Glu 
1               5                   10                  15      


Val Phe Asn Lys Asn Lys Tyr Thr Leu Thr Asn Gln Glu Asn Lys Asp 
            20                  25                  30          


Val Thr Glu Leu Trp Leu Gln Ile Leu Lys Gly Leu Lys Phe Pro Asn 
        35                  40                  45              


Glu Leu Lys Glu Thr Val Ser Tyr Ser Lys Asn Leu Lys Glu Leu Ser 
    50                  55                  60                  


Leu Lys Thr His Ala Glu Val Ser Val Cys Ile Ile Ala Lys Asn Glu 
65                  70                  75                  80  


Gln Asp Ser Ile Arg Lys Cys Ile Asn Ser Ile Tyr Glu Phe Ser Asp 
                85                  90                  95      


Glu Ile Ile Phe Ile Asp Thr Gly Ser Ile Asp Ser Thr Lys Lys Ile 
            100                 105                 110         


Val Lys Glu Ile Ala Ser Glu Lys Val Lys Ile Phe Asp Tyr Thr Trp 
        115                 120                 125             


Gln Asp Asp Phe Ser Asp Ala Arg Asn Tyr Ser Ile Gln Lys Ala Ser 
    130                 135                 140                 


Lys Glu Trp Ile Leu Ile Ile Asp Ala Asp Glu Tyr Val Ser Ser Asp 
145                 150                 155                 160 


Glu Leu Ile Lys Leu Arg Leu Leu Ile Asp Met Leu Asp Arg Phe Lys 
                165                 170                 175     


Phe Lys Asp Ser Leu Arg Val Ser Cys Ala Ile Tyr Gln Leu Asp Asn 
            180                 185                 190         


Val Ile Thr His Gly Gln Ser Arg Leu Phe Arg Asn Asn Asn Lys Ile 
        195                 200                 205             


Lys Tyr Tyr Gly Leu Ile His Glu Glu Leu Arg Asn Asn Lys Gly Leu 
    210                 215                 220                 


Asp Pro Ile Phe Asn Val Glu Ser Glu Ile Thr Phe Phe His Asp Gly 
225                 230                 235                 240 


Tyr Lys Glu Ile Leu Arg Lys Glu Lys Cys Glu Arg Asn Ile Arg Leu 
                245                 250                 255     


Leu Ala Lys Met Leu Glu Lys Glu Pro Asp Asn Val Arg Trp Ala Tyr 
            260                 265                 270         


Leu Tyr Cys Arg Asp Ser Phe Ser Ile Asn Ser Asn Ile Asp Phe Glu 
        275                 280                 285             


Lys Ile Leu Leu Pro Phe Leu Ile Lys Asn Met Asp Glu Ser Ile Ser 
    290                 295                 300                 


Cys Glu Asn Ile Leu Leu Thr Asn Tyr Thr His Leu Ile Leu Phe Leu 
305                 310                 315                 320 


Ile Thr Lys Lys Tyr Ile Ile Asp Gly Lys Ser Ser Leu Ala Ser Lys 
                325                 330                 335     


Cys Ile Glu Val Leu Glu Lys Met Leu Pro Asn Ser Ser Asp Val Thr 
            340                 345                 350         


Phe Tyr Lys Phe Leu Asn Lys Gln His Ser Leu Tyr Glu Gln Gln Phe 
        355                 360                 365             


Glu Phe Leu Lys Glu Val Ile Gln Phe Arg Lys Asn Asn Glu Tyr Asp 
    370                 375                 380                 


Gln Tyr Ser Gln Ile Gly Cys Asn Leu Leu His Tyr Asp Leu Leu Ile 
385                 390                 395                 400 


Ser Gly Leu Leu Phe Asp Val Lys Ser Tyr Asp Tyr Ser Tyr Gln Tyr 
                405                 410                 415     


Phe Leu Lys Leu Asp Leu Ala Asn Tyr Phe Ser Glu Leu Glu Ile Pro 
            420                 425                 430         


Asp Glu Tyr Lys Met Leu Ile Asn Lys Tyr Arg Glu Asn Glu Ser Leu 
        435                 440                 445             


Glu His His His His His His 
    450                 455 


<210>  2
<211>  469
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  MISC_FEATURE
<223>  N-term 6X His tagged recombinant Glycosyltransferase protein

<400>  2

Met His His His His His His Ser Ser Gly Val Asp Leu Gly Thr Glu 
1               5                   10                  15      


Asn Leu Tyr Phe Gln Ser Met Gly Tyr Ser Glu Asn Phe Ile Ala Asn 
            20                  25                  30          


Asp Trp Phe Asn Val Glu Val Phe Asn Lys Asn Lys Tyr Thr Leu Thr 
        35                  40                  45              


Asn Gln Glu Asn Lys Asp Val Thr Glu Leu Trp Leu Gln Ile Leu Lys 
    50                  55                  60                  


Gly Leu Lys Phe Pro Asn Glu Leu Lys Glu Thr Val Ser Tyr Ser Lys 
65                  70                  75                  80  


Asn Leu Lys Glu Leu Ser Leu Lys Thr His Ala Glu Val Ser Val Cys 
                85                  90                  95      


Ile Ile Ala Lys Asn Glu Gln Asp Ser Ile Arg Lys Cys Ile Asn Ser 
            100                 105                 110         


Ile Tyr Glu Phe Ser Asp Glu Ile Ile Phe Ile Asp Thr Gly Ser Ile 
        115                 120                 125             


Asp Ser Thr Lys Lys Ile Val Lys Glu Ile Ala Ser Glu Lys Val Lys 
    130                 135                 140                 


Ile Phe Asp Tyr Thr Trp Gln Asp Asp Phe Ser Asp Ala Arg Asn Tyr 
145                 150                 155                 160 


Ser Ile Gln Lys Ala Ser Lys Glu Trp Ile Leu Ile Ile Asp Ala Asp 
                165                 170                 175     


Glu Tyr Val Ser Ser Asp Glu Leu Ile Lys Leu Arg Leu Leu Ile Asp 
            180                 185                 190         


Met Leu Asp Arg Phe Lys Phe Lys Asp Ser Leu Arg Val Ser Cys Ala 
        195                 200                 205             


Ile Tyr Gln Leu Asp Asn Val Ile Thr His Gly Gln Ser Arg Leu Phe 
    210                 215                 220                 


Arg Asn Asn Asn Lys Ile Lys Tyr Tyr Gly Leu Ile His Glu Glu Leu 
225                 230                 235                 240 


Arg Asn Asn Lys Gly Leu Asp Pro Ile Phe Asn Val Glu Ser Glu Ile 
                245                 250                 255     


Thr Phe Phe His Asp Gly Tyr Lys Glu Ile Leu Arg Lys Glu Lys Cys 
            260                 265                 270         


Glu Arg Asn Ile Arg Leu Leu Ala Lys Met Leu Glu Lys Glu Pro Asp 
        275                 280                 285             


Asn Val Arg Trp Ala Tyr Leu Tyr Cys Arg Asp Ser Phe Ser Ile Asn 
    290                 295                 300                 


Ser Asn Ile Asp Phe Glu Lys Ile Leu Leu Pro Phe Leu Ile Lys Asn 
305                 310                 315                 320 


Met Asp Glu Ser Ile Ser Cys Glu Asn Ile Leu Leu Thr Asn Tyr Thr 
                325                 330                 335     


His Leu Ile Leu Phe Leu Ile Thr Lys Lys Tyr Ile Ile Asp Gly Lys 
            340                 345                 350         


Ser Ser Leu Ala Ser Lys Cys Ile Glu Val Leu Glu Lys Met Leu Pro 
        355                 360                 365             


Asn Ser Ser Asp Val Thr Phe Tyr Lys Phe Leu Asn Lys Gln His Ser 
    370                 375                 380                 


Leu Tyr Glu Gln Gln Phe Glu Phe Leu Lys Glu Val Ile Gln Phe Arg 
385                 390                 395                 400 


Lys Asn Asn Glu Tyr Asp Gln Tyr Ser Gln Ile Gly Cys Asn Leu Leu 
                405                 410                 415     


His Tyr Asp Leu Leu Ile Ser Gly Leu Leu Phe Asp Val Lys Ser Tyr 
            420                 425                 430         


Asp Tyr Ser Tyr Gln Tyr Phe Leu Lys Leu Asp Leu Ala Asn Tyr Phe 
        435                 440                 445             


Ser Glu Leu Glu Ile Pro Asp Glu Tyr Lys Met Leu Ile Asn Lys Tyr 
    450                 455                 460                 


Arg Glu Asn Glu Ser 
465                 


<210>  3
<211>  819
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  N-term MBP-Glycosyltransferase fusion protein

<400>  3

Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys 
1               5                   10                  15      


Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr 
            20                  25                  30          


Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe 
        35                  40                  45              


Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala 
    50                  55                  60                  


His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile 
65                  70                  75                  80  


Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp 
                85                  90                  95      


Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu 
            100                 105                 110         


Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys 
        115                 120                 125             


Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly 
    130                 135                 140                 


Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 
145                 150                 155                 160 


Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys 
                165                 170                 175     


Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly 
            180                 185                 190         


Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp 
        195                 200                 205             


Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala 
    210                 215                 220                 


Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 
225                 230                 235                 240 


Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser 
                245                 250                 255     


Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro 
            260                 265                 270         


Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp 
        275                 280                 285             


Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala 
    290                 295                 300                 


Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 
305                 310                 315                 320 


Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln 
                325                 330                 335     


Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala 
            340                 345                 350         


Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Ala Ala Ala Gln Thr Asn 
        355                 360                 365             


Ala Ala His Met Tyr Ser Glu Asn Phe Ile Ala Asn Asp Trp Phe Asn 
    370                 375                 380                 


Val Glu Val Phe Asn Lys Asn Lys Tyr Thr Leu Thr Asn Gln Glu Asn 
385                 390                 395                 400 


Lys Asp Val Thr Glu Leu Trp Leu Gln Ile Leu Lys Gly Leu Lys Phe 
                405                 410                 415     


Pro Asn Glu Leu Lys Glu Thr Val Ser Tyr Ser Lys Asn Leu Lys Glu 
            420                 425                 430         


Leu Ser Leu Lys Thr His Ala Glu Val Ser Val Cys Ile Ile Ala Lys 
        435                 440                 445             


Asn Glu Gln Asp Ser Ile Arg Lys Cys Ile Asn Ser Ile Tyr Glu Phe 
    450                 455                 460                 


Ser Asp Glu Ile Ile Phe Ile Asp Thr Gly Ser Ile Asp Ser Thr Lys 
465                 470                 475                 480 


Lys Ile Val Lys Glu Ile Ala Ser Glu Lys Val Lys Ile Phe Asp Tyr 
                485                 490                 495     


Thr Trp Gln Asp Asp Phe Ser Asp Ala Arg Asn Tyr Ser Ile Gln Lys 
            500                 505                 510         


Ala Ser Lys Glu Trp Ile Leu Ile Ile Asp Ala Asp Glu Tyr Val Ser 
        515                 520                 525             


Ser Asp Glu Leu Ile Lys Leu Arg Leu Leu Ile Asp Met Leu Asp Arg 
    530                 535                 540                 


Phe Lys Phe Lys Asp Ser Leu Arg Val Ser Cys Ala Ile Tyr Gln Leu 
545                 550                 555                 560 


Asp Asn Val Ile Thr His Gly Gln Ser Arg Leu Phe Arg Asn Asn Asn 
                565                 570                 575     


Lys Ile Lys Tyr Tyr Gly Leu Ile His Glu Glu Leu Arg Asn Asn Lys 
            580                 585                 590         


Gly Leu Asp Pro Ile Phe Asn Val Glu Ser Glu Ile Thr Phe Phe His 
        595                 600                 605             


Asp Gly Tyr Lys Glu Ile Leu Arg Lys Glu Lys Cys Glu Arg Asn Ile 
    610                 615                 620                 


Arg Leu Leu Ala Lys Met Leu Glu Lys Glu Pro Asp Asn Val Arg Trp 
625                 630                 635                 640 


Ala Tyr Leu Tyr Cys Arg Asp Ser Phe Ser Ile Asn Ser Asn Ile Asp 
                645                 650                 655     


Phe Glu Lys Ile Leu Leu Pro Phe Leu Ile Lys Asn Met Asp Glu Ser 
            660                 665                 670         


Ile Ser Cys Glu Asn Ile Leu Leu Thr Asn Tyr Thr His Leu Ile Leu 
        675                 680                 685             


Phe Leu Ile Thr Lys Lys Tyr Ile Ile Asp Gly Lys Ser Ser Leu Ala 
    690                 695                 700                 


Ser Lys Cys Ile Glu Val Leu Glu Lys Met Leu Pro Asn Ser Ser Asp 
705                 710                 715                 720 


Val Thr Phe Tyr Lys Phe Leu Asn Lys Gln His Ser Leu Tyr Glu Gln 
                725                 730                 735     


Gln Phe Glu Phe Leu Lys Glu Val Ile Gln Phe Arg Lys Asn Asn Glu 
            740                 745                 750         


Tyr Asp Gln Tyr Ser Gln Ile Gly Cys Asn Leu Leu His Tyr Asp Leu 
        755                 760                 765             


Leu Ile Ser Gly Leu Leu Phe Asp Val Lys Ser Tyr Asp Tyr Ser Tyr 
    770                 775                 780                 


Gln Tyr Phe Leu Lys Leu Asp Leu Ala Asn Tyr Phe Ser Glu Leu Glu 
785                 790                 795                 800 


Ile Pro Asp Glu Tyr Lys Met Leu Ile Asn Lys Tyr Arg Glu Asn Glu 
                805                 810                 815     


Ser Leu Glu 
            


<210>  4
<211>  48
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 1

<400>  4

Met Ser Lys Arg Asp Cys Asn Leu Met Lys Ala Cys Cys Ala Gly Gln 
1               5                   10                  15      


Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
            20                  25                  30          


Ser Ser Asp Pro Ala Gly Cys Asn Asp Ile Val Arg Lys Tyr Cys Lys 
        35                  40                  45              


<210>  5
<211>  48
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 2

<400>  5

Met Ser Lys Arg Asp Cys Asn Leu Met Lys Ala Cys Cys Ala Gly Gln 
1               5                   10                  15      


Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
            20                  25                  30          


Cys Ser Asp Pro Ala Gly Cys Asn Asp Ile Val Arg Lys Tyr Cys Lys 
        35                  40                  45              


<210>  6
<211>  36
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 3

<400>  6

Met Ser Lys Arg Asp Cys Asn Leu Met Lys Ala Cys Cys Ala Gly Gln 
1               5                   10                  15      


Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
            20                  25                  30          


Ser Ser Asp Pro 
        35      


<210>  7
<211>  32
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 4

<400>  7

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
1               5                   10                  15      


Ser Ser Asp Pro Ala Gly Cys Asn Asp Ile Val Arg Lys Tyr Cys Lys 
            20                  25                  30          


<210>  8
<211>  20
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 5

<400>  8

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
1               5                   10                  15      


Ser Ser Asp Pro 
            20  


<210>  9
<211>  20
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 6

<400>  9

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
1               5                   10                  15      


Thr Ser Asp Pro 
            20  


<210>  10
<211>  20
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 7

<400>  10

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Glu 
1               5                   10                  15      


Ser Ser Asp Pro 
            20  


<210>  11
<211>  19
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 8

<400>  11

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
1               5                   10                  15      


Ser Ala Pro 
            


<210>  12
<211>  19
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 9

<400>  12

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
1               5                   10                  15      


Ser Ser Pro 
            


<210>  13
<211>  19
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 10

<400>  13

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
1               5                   10                  15      


Ser Arg Pro 
            


<210>  14
<211>  19
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 11

<400>  14

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
1               5                   10                  15      


Ser Thr Pro 
            


<210>  15
<211>  18
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 12

<400>  15

Ala Val Thr Tyr Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp 
1               5                   10                  15      


Ser Ser 
        


<210>  16
<211>  16
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 13

<400>  16

Ala Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp Ser Ser Asp Pro 
1               5                   10                  15      


<210>  17
<211>  15
<212>  PRT
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Acceptor Substrate 14

<400>  17

Ile His Ser Leu Leu Asn Arg Leu Gly Gly Asp Ser Ser Asp Pro 
1               5                   10                  15  


<210>  18
<211>  1343
<212>  DNA
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Expression cassette encoding  GT-CTH

<400>  18
catgggctat tctgaaaatt ttattgctaa tgactggttt aatgtagagg tatttaataa       60

aaataagtat actttaacga accaagagaa taaagatgta acagaattat ggttacaaat      120

tttaaaaggg ctaaagttcc ccaacgaatt aaaggaaact gtcagttact ctaaaaattt      180

aaaagaatta tctttaaaaa ctcacgcaga agtatctgta tgtattattg ctaagaatga      240

acaggattca ataagaaaat gtattaatag tatctatgaa ttttcagatg aaattatatt      300

tattgataca ggatcaattg attcgacaaa aaaaatagta aaagaaatag caagcgaaaa      360

agtaaaaata tttgattata cttggcaaga tgatttttca gatgcgagaa attattcaat      420

acaaaaagca agtaaagaat ggatattaat tattgatgca gatgaatatg tatcttcaga      480

tgagcttatc aaattaaggc tcttaataga tatgttagac aggtttaaat ttaaagattc      540

attaagagtt agttgtgcaa tatatcaatt agataatgtt atcacacatg gccaaagtcg      600

attatttaga aacaataata aaattaagta ttatggtcta atacatgaag agttgaggaa      660

caacaaagga ttagatccaa tttttaacgt tgaaagtgag attacttttt tccatgacgg      720

ttacaaagaa atacttagga aagagaagtg tgaaagaaac ataaggctac tagctaagat      780

gttagaaaaa gagccagaca atgttagatg ggcatacttg tattgtagag attcattttc      840

tataaattcc aacattgatt ttgaaaaaat tctacttcca tttttaataa agaatatgga      900

tgaaagtata tcatgtgaga atatcctact tacaaactat actcatttaa tcctatttct      960

tattactaag aaatatataa ttgatgggaa aagctcactt gcaagtaaat gtatagaggt     1020

gttagaaaaa atgctaccta actcttctga tgttactttt tacaaatttt taaataaaca     1080

gcatagtttg tatgaacaac aatttgaatt tttaaaagaa gtaattcaat ttagaaaaaa     1140

taatgaatat gatcaatata gccaaatagg gtgtaattta ttacactatg atttattaat     1200

ttcaggatta ctttttgatg ttaagtctta tgattattca tatcaatact ttttaaaatt     1260

agatttagct aactattttt ctgaattaga gattcctgat gaatacaaaa tgttaataaa     1320

taagtatcgg gagaatgaat cac                                             1343


<210>  19
<211>  1346
<212>  DNA
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Expression cassette encoding MBP-NTH

<400>  19
catgggctat tctgaaaatt ttattgctaa tgactggttt aatgtagagg tatttaataa       60

aaataagtat actttaacga accaagagaa taaagatgta acagaattat ggttacaaat      120

tttaaaaggg ctaaagttcc ccaacgaatt aaaggaaact gtcagttact ctaaaaattt      180

aaaagaatta tctttaaaaa ctcacgcaga agtatctgta tgtattattg ctaagaatga      240

acaggattca ataagaaaat gtattaatag tatctatgaa ttttcagatg aaattatatt      300

tattgataca ggatcaattg attcgacaaa aaaaatagta aaagaaatag caagcgaaaa      360

agtaaaaata tttgattata cttggcaaga tgatttttca gatgcgagaa attattcaat      420

acaaaaagca agtaaagaat ggatattaat tattgatgca gatgaatatg tatcttcaga      480

tgagcttatc aaattaaggc tcttaataga tatgttagac aggtttaaat ttaaagattc      540

attaagagtt agttgtgcaa tatatcaatt agataatgtt atcacacatg gccaaagtcg      600

attatttaga aacaataata aaattaagta ttatggtcta atacatgaag agttgaggaa      660

caacaaagga ttagatccaa tttttaacgt tgaaagtgag attacttttt tccatgacgg      720

ttacaaagaa atacttagga aagagaagtg tgaaagaaac ataaggctac tagctaagat      780

gttagaaaaa gagccagaca atgttagatg ggcatacttg tattgtagag attcattttc      840

tataaattcc aacattgatt ttgaaaaaat tctacttcca tttttaataa agaatatgga      900

tgaaagtata tcatgtgaga atatcctact tacaaactat actcatttaa tcctatttct      960

tattactaag aaatatataa ttgatgggaa aagctcactt gcaagtaaat gtatagaggt     1020

gttagaaaaa atgctaccta actcttctga tgttactttt tacaaatttt taaataaaca     1080

gcatagtttg tatgaacaac aatttgaatt tttaaaagaa gtaattcaat ttagaaaaaa     1140

taatgaatat gatcaatata gccaaatagg gtgtaattta ttacactatg atttattaat     1200

ttcaggatta ctttttgatg ttaagtctta tgattattca tatcaatact ttttaaaatt     1260

agatttagct aactattttt ctgaattaga gattcctgat gaatacaaaa tgttaataaa     1320

taagtatcgg gagaatgaat catgac                                          1346


<210>  20
<211>  1340
<212>  DNA
<213>  SYNTHETIC


<220>
<221>  misc_feature
<223>  Expression cassette encoding MBP-GT

<400>  20
tatgtattct gaaaatttta ttgctaatga ctggtttaat gtagaggtat ttaataaaaa       60

taagtatact ttaacgaacc aagagaataa agatgtaaca gaattatggt tacaaatttt      120

aaaagggcta aagttcccca acgaattaaa ggaaactgtc agttactcta aaaatttaaa      180

agaattatct ttaaaaactc acgcagaagt atctgtatgt attattgcta agaatgaaca      240

ggattcaata agaaaatgta ttaatagtat ctatgaattt tcagatgaaa ttatatttat      300

tgatacagga tcaattgatt cgacaaaaaa aatagtaaaa gaaatagcaa gcgaaaaagt      360

aaaaatattt gattatactt ggcaagatga tttttcagat gcgagaaatt attcaataca      420

aaaagcaagt aaagaatgga tattaattat tgatgcagat gaatatgtat cttcagatga      480

gcttatcaaa ttaaggctct taatagatat gttagacagg tttaaattta aagattcatt      540

aagagttagt tgtgcaatat atcaattaga taatgttatc acacatggcc aaagtcgatt      600

atttagaaac aataataaaa ttaagtatta tggtctaata catgaagagt tgaggaacaa      660

caaaggatta gatccaattt ttaacgttga aagtgagatt acttttttcc atgacggtta      720

caaagaaata cttaggaaag agaagtgtga aagaaacata aggctactag ctaagatgtt      780

agaaaaagag ccagacaatg ttagatgggc atacttgtat tgtagagatt cattttctat      840

aaattccaac attgattttg aaaaaattct acttccattt ttaataaaga atatggatga      900

aagtatatca tgtgagaata tcctacttac aaactatact catttaatcc tatttcttat      960

tactaagaaa tatataattg atgggaaaag ctcacttgca agtaaatgta tagaggtgtt     1020

agaaaaaatg ctacctaact cttctgatgt tactttttac aaatttttaa ataaacagca     1080

tagtttgtat gaacaacaat ttgaattttt aaaagaagta attcaattta gaaaaaataa     1140

tgaatatgat caatatagcc aaatagggtg taatttatta cactatgatt tattaatttc     1200

aggattactt tttgatgtta agtcttatga ttattcatat caatactttt taaaattaga     1260

tttagctaac tatttttctg aattagagat tcctgatgaa tacaaaatgt taataaataa     1320

gtatcgggag aatgaatcac                                                 1340



