                         SEQUENCE LISTING



<110>  Universitat Pompeu Fabra

 

<120>  CONTROL OF CANCER PROGRESSION BY RETINOBLASTOMA PHOSPHORYLATION



<130>  PC10162PC00



<160>  59    



<170>  PatentIn version 3.5



<210>  1

<211>  36

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer for PCR amplification of GST-RB



<400>  1

catcatcatg gatccatgcc gcccaaaacc ccccga                                 36





<210>  2

<211>  33

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer for PCR amplification of GST-RB



<400>  2

catcatcatg gatcctcatt gttggatagt ggt                                    33





<210>  3

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer for PCR amplification of full-length RB from pGEX2TK-RB



<400>  3

catggatcca tgccgcccaa aaccccc                                           27





<210>  4

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Primer for PCR amplification of full-length RB from pGEX2TK-RB



<400>  4

catctcgagt catttctctt ccttgtt                                           27





<210>  5

<211>  30

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair T5A



<400>  5

atgccgccca aagccccccg aaaaacgtac                                        30





<210>  6

<211>  24

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair T5A



<400>  6

ggcgggtttc ggggggcttt ttgc                                              24





<210>  7

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair S230A



<400>  7

tttattaaac tcgcacctcc catgttg                                           27





<210>  8

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair S230A



<400>  8

aaataatttg agcgtggagg gtacaac                                           27





<210>  9

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair S249A



<400>  9

cccattaatg gtgcacctcg aacaccc                                           27





<210>  10

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair S249A



<400>  10

gggtaattac cacgtggagc ttgtggg                                           27





<210>  11

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair T252A



<400>  11

ggttcacctc gagcacccag gcgaggt                                           27





<210>  12

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair T252A



<400>  12

ccaagtggag ctcgtgggtc cgctcca                                           27





<210>  13

<211>  48

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair S249AT252A



<400>  13

gttataccca ttaatggtgc acctcgagca cccaggcgag gtcagaac                    48





<210>  14

<211>  48

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair S249AT252A



<400>  14

caatatgggt aattaccacg tggagctcgt gggtccgctc cagtcttg                    48





<210>  15

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair 249A252E



<400>  15

ggtgcacctc gagaacccag gcgaggt                                           27





<210>  16

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair 249A252E



<400>  16

ccacgtggag ctcttgggtc cgctcca                                           27





<210>  17

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair 249E252E



<400>  17

cccattaatg gtgaacctcg agaaccc                                           27





<210>  18

<211>  27

<212>  DNA

<213>  Artificial sequence



<220>

<223>  RB mutagenesis primer of pair 249E252E



<400>  18

gggtaattac cacttggagc tcttggg                                           27





<210>  19

<211>  30

<212>  DNA

<213>  Artificial sequence



<220>

<223>  primer for PCR amplification of p38alpha from pMT3-HA-p38



<400>  19

gctagtggat tcatgtctca ggagaggccc                                        30





<210>  20

<211>  30

<212>  DNA

<213>  Artificial sequence



<220>

<223>  primer for PCR amplification of p38alpha from pMT3-HA-p38



<400>  20

gtcgatctcg agtcaggact ccatctcttc                                        30





<210>  21

<211>  15

<212>  PRT

<213>  Artificial sequence



<220>

<223>  Fragment of human RB not phosphorylated at S249/T252





<220>

<221>  source

<222>  (1)..(15)

<223>  Homo sapiens, RB protein



<220>

<221>  note

<222>  (3)..(3)

<223>  Phosphorylatable Serine



<220>

<221>  note

<222>  (6)..(6)

<223>  Phosphorylatable Threonine



<400>  21



Asn Gly Ser Pro Arg Thr Pro Arg Arg Gly Gln Asn Arg Ser Cys 

1               5                   10                  15  





<210>  22

<211>  15

<212>  PRT

<213>  Artificial sequence



<220>

<223>  peptide used for generating antibodies against human RB 

       phosphorylated at Ser249/Thr252





<220>

<221>  source

<222>  (1)..(15)

<223>  Homo sapiens, RB protein



<220>

<221>  note

<222>  (3)..(3)

<223>  phosphorylated serine residue



<220>

<221>  note

<222>  (6)..(6)

<223>  phosphorylated threonine residue



<400>  22



Asn Gly Ser Pro Arg Thr Pro Arg Arg Gly Gln Asn Arg Ser Cys 

1               5                   10                  15  





<210>  23

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for mad2



<400>  23

aggatgaaat tcgctcag                                                     18





<210>  24

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for mad2



<400>  24

cattgacagg ggttttgt                                                     18





<210>  25

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for E2F1



<400>  25

ggatctggag actgacca                                                     18





<210>  26

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for E2F1



<400>  26

ctccaggaca ttggtgat                                                     18





<210>  27

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for BRCA1



<400>  27

actgaaaggc atccagaa                                                     18





<210>  28

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for BRCA1



<400>  28

gaacctgcct gtcgttac                                                     18





<210>  29

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for CycA2



<400>  29

gtccttcatg gaaagcag                                                     18





<210>  30

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for CycA2



<400>  30

acgttcactg gcttgtct                                                     18





<210>  31

<211>  20

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for CycE1



<400>  31

cctccaaagt tgcaccagtt                                                   20





<210>  32

<211>  20

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for CycE1



<400>  32

ggacgcacag gtctagaagc                                                   20





<210>  33

<211>  21

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for GAPDH



<400>  33

aattcaacgg cacagtcaag g                                                 21





<210>  34

<211>  21

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Real-time PCR primer for GAPDH



<400>  34

ggatgcaggg atgatgttct g                                                 21





<210>  35

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  PCR primer for human CycA2



<400>  35

ccccagccag tttgtttc                                                     18





<210>  36

<211>  12

<212>  DNA

<213>  Artificial sequence



<220>

<223>  PCR primer for human CycA2



<400>  36

tcaagttcat ag                                                           12





<210>  37

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  PCR primer for human E2F1



<400>  37

gcgttaaagc caatagga                                                     18





<210>  38

<211>  17

<212>  DNA

<213>  Artificial sequence



<220>

<223>  PCR primer for human E2F1



<400>  38

cttttacgcg ccaaatc                                                      17





<210>  39

<211>  19

<212>  DNA

<213>  Artificial sequence



<220>

<223>  PCR primer for mouse CycA2



<400>  39

ctcccgccct gtaagattc                                                    19





<210>  40

<211>  20

<212>  DNA

<213>  Artificial sequence



<220>

<223>  PCR primer for mouse CycA2



<400>  40

agttcaagta tcccgcgact                                                   20





<210>  41

<211>  18

<212>  DNA

<213>  Artificial sequence



<220>

<223>  PCR primer for mouse CycE1



<400>  41

cgcaggccct gacatcta                                                     18





<210>  42

<211>  17

<212>  DNA

<213>  Artificial sequence



<220>

<223>  PCR primer for mouse CycE1



<400>  42

accagcgagg agctctg                                                      17





<210>  43

<211>  10

<212>  DNA

<213>  Artificial sequence



<220>

<223>  E2F binding sequence, including two additional nucleotides that 

       can follow it





<220>

<221>  note

<222>  (4)..(5)

<223>  S can be G or C



<220>

<221>  note

<222>  (9)..(9)

<223>  N can be A, T, G or C (added for incluiding it in the sequence 

       listing)



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  n is a, c, g, or t



<220>

<221>  note

<222>  (10)..(10)

<223>  N can be A, T, G or C (added for including the motif in the 

       sequence listing)



<400>  43

tttsscgcnn                                                              10





<210>  44

<211>  51

<212>  DNA

<213>  Artificial sequence



<220>

<223>  TATA oligonucleotide used for the construction of an 

       E2F-dependent promoter



<400>  44

gatctacttg ggcataaaag gcagagcagg ggcagctgct gcttacactt a                51





<210>  45

<211>  64

<212>  DNA

<213>  Artificial sequence



<220>

<223>  3xE2F oligonucleotide used for the construction of an 

       E2F-dependent promoter



<400>  45

ctgcaatttc gcgccaaact tgtgcaattt cgcgccaaac ttgtgcaatt tcgcgccaac       60



ttgc                                                                    64





<210>  46

<211>  1314

<212>  DNA

<213>  Homo sapiens





<220>

<221>  CDS

<222>  (1)..(1314)

<223>  Coding sequence of E2F1 human protein



<400>  46

atg gcc ttg gcc ggg gcc cct gcg ggc ggc cca tgc gcg ccg gcg ctg         48

Met Ala Leu Ala Gly Ala Pro Ala Gly Gly Pro Cys Ala Pro Ala Leu           

1               5                   10                  15                



gag gcc ctg ctc ggg gcc ggc gcg ctg cgg ctg ctc gac tcc tcg cag         96

Glu Ala Leu Leu Gly Ala Gly Ala Leu Arg Leu Leu Asp Ser Ser Gln           

            20                  25                  30                    



atc gtc atc atc tcc gcc gcg cag gac gcc agc gcc ccg ccg gct ccc        144

Ile Val Ile Ile Ser Ala Ala Gln Asp Ala Ser Ala Pro Pro Ala Pro           

        35                  40                  45                        



acc ggc ccc gcg gcg ccc gcc gcc ggc ccc tgc gac cct gac ctg ctg        192

Thr Gly Pro Ala Ala Pro Ala Ala Gly Pro Cys Asp Pro Asp Leu Leu           

    50                  55                  60                            



ctc ttc gcc aca ccg cag gcg ccc cgg ccc aca ccc agt gcg ccg cgg        240

Leu Phe Ala Thr Pro Gln Ala Pro Arg Pro Thr Pro Ser Ala Pro Arg           

65                  70                  75                  80            



ccc gcg ctc ggc cgc ccg ccg gtg aag cgg agg ctg gac ctg gaa act        288

Pro Ala Leu Gly Arg Pro Pro Val Lys Arg Arg Leu Asp Leu Glu Thr           

                85                  90                  95                



gac cat cag tac ctg gcc gag agc agt ggg cca gct cgg ggc aga ggc        336

Asp His Gln Tyr Leu Ala Glu Ser Ser Gly Pro Ala Arg Gly Arg Gly           

            100                 105                 110                   



cgc cat cca gga aaa ggt gtg aaa tcc ccg ggg gag aag tca cgc tat        384

Arg His Pro Gly Lys Gly Val Lys Ser Pro Gly Glu Lys Ser Arg Tyr           

        115                 120                 125                       



gag acc tca ctg aat ctg acc acc aag cgc ttc ctg gag ctg ctg agc        432

Glu Thr Ser Leu Asn Leu Thr Thr Lys Arg Phe Leu Glu Leu Leu Ser           

    130                 135                 140                           



cac tcg gct gac ggt gtc gtc gac ctg aac tgg gct gcc gag gtg ctg        480

His Ser Ala Asp Gly Val Val Asp Leu Asn Trp Ala Ala Glu Val Leu           

145                 150                 155                 160           



aag gtg cag aag cgg cgc atc tat gac atc acc aac gtc ctt gag ggc        528

Lys Val Gln Lys Arg Arg Ile Tyr Asp Ile Thr Asn Val Leu Glu Gly           

                165                 170                 175               



atc cag ctc att gcc aag aag tcc aag aac cac atc cag tgg ctg ggc        576

Ile Gln Leu Ile Ala Lys Lys Ser Lys Asn His Ile Gln Trp Leu Gly           

            180                 185                 190                   



agc cac acc aca gtg ggc gtc ggc gga cgg ctt gag ggg ttg acc cag        624

Ser His Thr Thr Val Gly Val Gly Gly Arg Leu Glu Gly Leu Thr Gln           

        195                 200                 205                       



gac ctc cga cag ctg cag gag agc gag cag cag ctg gac cac ctg atg        672

Asp Leu Arg Gln Leu Gln Glu Ser Glu Gln Gln Leu Asp His Leu Met           

    210                 215                 220                           



aat atc tgt act acg cag ctg cgc ctg ctc tcc gag gac act gac agc        720

Asn Ile Cys Thr Thr Gln Leu Arg Leu Leu Ser Glu Asp Thr Asp Ser           

225                 230                 235                 240           



cag cgc ctg gcc tac gtg acg tgt cag gac ctt cgt agc att gca gac        768

Gln Arg Leu Ala Tyr Val Thr Cys Gln Asp Leu Arg Ser Ile Ala Asp           

                245                 250                 255               



cct gca gag cag atg gtt atg gtg atc aaa gcc cct cct gag acc cag        816

Pro Ala Glu Gln Met Val Met Val Ile Lys Ala Pro Pro Glu Thr Gln           

            260                 265                 270                   



ctc caa gcc gtg gac tct tcg gag aac ttt cag atc tcc ctt aag agc        864

Leu Gln Ala Val Asp Ser Ser Glu Asn Phe Gln Ile Ser Leu Lys Ser           

        275                 280                 285                       



aaa caa ggc ccg atc gat gtt ttc ctg tgc cct gag gag acc gta ggt        912

Lys Gln Gly Pro Ile Asp Val Phe Leu Cys Pro Glu Glu Thr Val Gly           

    290                 295                 300                           



ggg atc agc cct ggg aag acc cca tcc cag gag gtc act tct gag gag        960

Gly Ile Ser Pro Gly Lys Thr Pro Ser Gln Glu Val Thr Ser Glu Glu           

305                 310                 315                 320           



gag aac agg gcc act gac tct gcc acc ata gtg tca cca cca cca tca       1008

Glu Asn Arg Ala Thr Asp Ser Ala Thr Ile Val Ser Pro Pro Pro Ser           

                325                 330                 335               



tct ccc ccc tca tcc ctc acc aca gat ccc agc cag tct cta ctc agc       1056

Ser Pro Pro Ser Ser Leu Thr Thr Asp Pro Ser Gln Ser Leu Leu Ser           

            340                 345                 350                   



ctg gag caa gaa ccg ctg ttg tcc cgg atg ggc agc ctg cgg gct ccc       1104

Leu Glu Gln Glu Pro Leu Leu Ser Arg Met Gly Ser Leu Arg Ala Pro           

        355                 360                 365                       



gtg gac gag gac cgc ctg tcc ccg ctg gtg gcg gcc gac tcg ctc ctg       1152

Val Asp Glu Asp Arg Leu Ser Pro Leu Val Ala Ala Asp Ser Leu Leu           

    370                 375                 380                           



gag cat gtg cgg gag gac ttc tcc ggc ctc ctc cct gag gag ttc atc       1200

Glu His Val Arg Glu Asp Phe Ser Gly Leu Leu Pro Glu Glu Phe Ile           

385                 390                 395                 400           



agc ctt tcc cca ccc cac gag gcc ctc gac tac cac ttc ggc ctc gag       1248

Ser Leu Ser Pro Pro His Glu Ala Leu Asp Tyr His Phe Gly Leu Glu           

                405                 410                 415               



gag ggc gag ggc atc aga gac ctc ttc gac tgt gac ttt ggg gac ctc       1296

Glu Gly Glu Gly Ile Arg Asp Leu Phe Asp Cys Asp Phe Gly Asp Leu           

            420                 425                 430                   



acc ccc ctg gat ttc tga                                               1314

Thr Pro Leu Asp Phe                                                       

        435                                                               





<210>  47

<211>  437

<212>  PRT

<213>  Homo sapiens



<400>  47



Met Ala Leu Ala Gly Ala Pro Ala Gly Gly Pro Cys Ala Pro Ala Leu 

1               5                   10                  15      





Glu Ala Leu Leu Gly Ala Gly Ala Leu Arg Leu Leu Asp Ser Ser Gln 

            20                  25                  30          





Ile Val Ile Ile Ser Ala Ala Gln Asp Ala Ser Ala Pro Pro Ala Pro 

        35                  40                  45              





Thr Gly Pro Ala Ala Pro Ala Ala Gly Pro Cys Asp Pro Asp Leu Leu 

    50                  55                  60                  





Leu Phe Ala Thr Pro Gln Ala Pro Arg Pro Thr Pro Ser Ala Pro Arg 

65                  70                  75                  80  





Pro Ala Leu Gly Arg Pro Pro Val Lys Arg Arg Leu Asp Leu Glu Thr 

                85                  90                  95      





Asp His Gln Tyr Leu Ala Glu Ser Ser Gly Pro Ala Arg Gly Arg Gly 

            100                 105                 110         





Arg His Pro Gly Lys Gly Val Lys Ser Pro Gly Glu Lys Ser Arg Tyr 

        115                 120                 125             





Glu Thr Ser Leu Asn Leu Thr Thr Lys Arg Phe Leu Glu Leu Leu Ser 

    130                 135                 140                 





His Ser Ala Asp Gly Val Val Asp Leu Asn Trp Ala Ala Glu Val Leu 

145                 150                 155                 160 





Lys Val Gln Lys Arg Arg Ile Tyr Asp Ile Thr Asn Val Leu Glu Gly 

                165                 170                 175     





Ile Gln Leu Ile Ala Lys Lys Ser Lys Asn His Ile Gln Trp Leu Gly 

            180                 185                 190         





Ser His Thr Thr Val Gly Val Gly Gly Arg Leu Glu Gly Leu Thr Gln 

        195                 200                 205             





Asp Leu Arg Gln Leu Gln Glu Ser Glu Gln Gln Leu Asp His Leu Met 

    210                 215                 220                 





Asn Ile Cys Thr Thr Gln Leu Arg Leu Leu Ser Glu Asp Thr Asp Ser 

225                 230                 235                 240 





Gln Arg Leu Ala Tyr Val Thr Cys Gln Asp Leu Arg Ser Ile Ala Asp 

                245                 250                 255     





Pro Ala Glu Gln Met Val Met Val Ile Lys Ala Pro Pro Glu Thr Gln 

            260                 265                 270         





Leu Gln Ala Val Asp Ser Ser Glu Asn Phe Gln Ile Ser Leu Lys Ser 

        275                 280                 285             





Lys Gln Gly Pro Ile Asp Val Phe Leu Cys Pro Glu Glu Thr Val Gly 

    290                 295                 300                 





Gly Ile Ser Pro Gly Lys Thr Pro Ser Gln Glu Val Thr Ser Glu Glu 

305                 310                 315                 320 





Glu Asn Arg Ala Thr Asp Ser Ala Thr Ile Val Ser Pro Pro Pro Ser 

                325                 330                 335     





Ser Pro Pro Ser Ser Leu Thr Thr Asp Pro Ser Gln Ser Leu Leu Ser 

            340                 345                 350         





Leu Glu Gln Glu Pro Leu Leu Ser Arg Met Gly Ser Leu Arg Ala Pro 

        355                 360                 365             





Val Asp Glu Asp Arg Leu Ser Pro Leu Val Ala Ala Asp Ser Leu Leu 

    370                 375                 380                 





Glu His Val Arg Glu Asp Phe Ser Gly Leu Leu Pro Glu Glu Phe Ile 

385                 390                 395                 400 





Ser Leu Ser Pro Pro His Glu Ala Leu Asp Tyr His Phe Gly Leu Glu 

                405                 410                 415     





Glu Gly Glu Gly Ile Arg Asp Leu Phe Asp Cys Asp Phe Gly Asp Leu 

            420                 425                 430         





Thr Pro Leu Asp Phe 

        435         





<210>  48

<211>  9

<212>  PRT

<213>  Artificial sequence



<220>

<223>  HA-tag, from human influenza hemagglutinin



<400>  48



Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 

1               5                   





<210>  49

<211>  10

<212>  PRT

<213>  Artificial sequence



<220>

<223>  Myc-tag



<400>  49



Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 

1               5                   10  





<210>  50

<211>  723

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Coding sequence of GST tag





<220>

<221>  CDS

<222>  (1)..(723)

<223>  glutathione S-transferase



<400>  50

atg tcc cct ata cta ggt tat tgg aaa att aag ggc ctt gtg caa ccc         48

Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro           

1               5                   10                  15                



act cga ctt ctt ttg gaa tat ctt gaa gaa aaa tat gaa gag cat ttg         96

Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu           

            20                  25                  30                    



tat gag cgc gat gaa ggt gat aaa tgg cga aac aaa aag ttt gaa ttg        144

Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu           

        35                  40                  45                        



ggt ttg gag ttt ccc aat ctt cct tat tat att gat ggt gat gtt aaa        192

Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys           

    50                  55                  60                            



tta aca cag tct atg gcc atc ata cgt tat ata gct gac aag cac aac        240

Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn           

65                  70                  75                  80            



atg ttg ggt ggt tgt cca aaa gag cgt gca gag att tca atg ctt gaa        288

Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu           

                85                  90                  95                



gga gcg gtt ttg gat att aga tac ggt gtt tcg aga att gca tat agt        336

Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser           

            100                 105                 110                   



aaa gac ttt gaa act ctc aaa gtt gat ttt ctt agc aag cta cct gaa        384

Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu           

        115                 120                 125                       



atg ctg aaa atg ttc gaa gat cgt tta tgt cat aaa aca tat tta aat        432

Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn           

    130                 135                 140                           



ggt gat cat gta acc cat cct gac ttc atg ttg tat gac gct ctt gat        480

Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp           

145                 150                 155                 160           



gtt gtt tta tac atg gac cca atg tgc ctg gat gcg ttc cca aaa tta        528

Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu           

                165                 170                 175               



gtt tgt ttt aaa aaa cgt att gaa gct atc cca caa att gat aag tac        576

Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr           

            180                 185                 190                   



ttg aaa tcc agc aag tat ata gca tgg cct ttg cag ggc tgg caa gcc        624

Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala           

        195                 200                 205                       



acg ttt ggt ggt ggc gac cat cct cca aaa tcg gat ctg gtt ccg cgt        672

Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Val Pro Arg           

    210                 215                 220                           



gga tct cgt cgt gca tct gtt gga tcc ccg gga att cat cgt gac tga        720

Gly Ser Arg Arg Ala Ser Val Gly Ser Pro Gly Ile His Arg Asp               

225                 230                 235                               



ctg                                                                    723

Leu                                                                       

240                                                                       





<210>  51

<211>  239

<212>  PRT

<213>  Artificial sequence



<220>

<223>  Synthetic Construct



<400>  51



Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 

1               5                   10                  15      





Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 

            20                  25                  30          





Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 

        35                  40                  45              





Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 

    50                  55                  60                  





Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 

65                  70                  75                  80  





Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 

                85                  90                  95      





Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 

            100                 105                 110         





Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 

        115                 120                 125             





Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 

    130                 135                 140                 





Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 

145                 150                 155                 160 





Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 

                165                 170                 175     





Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 

            180                 185                 190         





Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 

        195                 200                 205             





Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Val Pro Arg 

    210                 215                 220                 





Gly Ser Arg Arg Ala Ser Val Gly Ser Pro Gly Ile His Arg Asp 

225                 230                 235                 





<210>  52

<211>  30

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Oligonucleotide for amplifying MKK6DD



<400>  52

gctagtgaat tcatgtctca gtcgaaaggc                                        30





<210>  53

<211>  30

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Oligonucleotide for amplifying MKK6DD



<400>  53

gtcgatgtcg acttagtctc caagaatcag                                        30





<210>  54

<211>  48

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Oligonucleotide encoding HA epitope



<400>  54

agcttaccat ggcctacccc tacgacgtgc ccgactacgc ctccctcg                    48





<210>  55

<211>  48

<212>  DNA

<213>  Artificial sequence



<220>

<223>  Oligonucleotide encoding HA epitope



<400>  55

gatccgaggg aggcgtagtc gggcacgtcg taggggtagg ccatggta                    48





<210>  56

<211>  2787

<212>  DNA

<213>  Homo sapiens





<220>

<221>  CDS

<222>  (1)..(2787)

<223>  RB



<400>  56

atg ccg ccc aaa acc ccc cga aaa acg gcc gcc acc gcc gcc gct gcc         48

Met Pro Pro Lys Thr Pro Arg Lys Thr Ala Ala Thr Ala Ala Ala Ala           

1               5                   10                  15                



gcc gcg gaa ccc ccg gca ccg ccg ccg ccg ccc cct cct gag gag gac         96

Ala Ala Glu Pro Pro Ala Pro Pro Pro Pro Pro Pro Pro Glu Glu Asp           

            20                  25                  30                    



cca gag cag gac agc ggc ccg gag gac ctg cct ctc gtc agg ctt gag        144

Pro Glu Gln Asp Ser Gly Pro Glu Asp Leu Pro Leu Val Arg Leu Glu           

        35                  40                  45                        



ttt gaa gaa aca gaa gaa cct gat ttt act gca tta tgt cag aaa tta        192

Phe Glu Glu Thr Glu Glu Pro Asp Phe Thr Ala Leu Cys Gln Lys Leu           

    50                  55                  60                            



aag ata cca gat cat gtc aga gag aga gct tgg tta act tgg gag aaa        240

Lys Ile Pro Asp His Val Arg Glu Arg Ala Trp Leu Thr Trp Glu Lys           

65                  70                  75                  80            



gtt tca tct gtg gat gga gta ttg gga ggt tat att caa aag aaa aag        288

Val Ser Ser Val Asp Gly Val Leu Gly Gly Tyr Ile Gln Lys Lys Lys           

                85                  90                  95                



gaa ctg tgg gga atc tgt atc ttt att gca gca gtt gac cta gat gag        336

Glu Leu Trp Gly Ile Cys Ile Phe Ile Ala Ala Val Asp Leu Asp Glu           

            100                 105                 110                   



atg tcg ttc act ttt act gag cta cag aaa aac ata gaa atc agt gtc        384

Met Ser Phe Thr Phe Thr Glu Leu Gln Lys Asn Ile Glu Ile Ser Val           

        115                 120                 125                       



cat aaa ttc ttt aac tta cta aaa gaa att gat acc agt acc aaa gtt        432

His Lys Phe Phe Asn Leu Leu Lys Glu Ile Asp Thr Ser Thr Lys Val           

    130                 135                 140                           



gat aat gct atg tca aga ctg ttg aag aag tat gat gta ttg ttt gca        480

Asp Asn Ala Met Ser Arg Leu Leu Lys Lys Tyr Asp Val Leu Phe Ala           

145                 150                 155                 160           



ctc ttc agc aaa ttg gaa agg aca tgt gaa ctt ata tat ttg aca caa        528

Leu Phe Ser Lys Leu Glu Arg Thr Cys Glu Leu Ile Tyr Leu Thr Gln           

                165                 170                 175               



ccc agc agt tcg ata tct act gaa ata aat tct gca ttg gtg cta aaa        576

Pro Ser Ser Ser Ile Ser Thr Glu Ile Asn Ser Ala Leu Val Leu Lys           

            180                 185                 190                   



gtt tct tgg atc aca ttt tta tta gct aaa ggg gaa gta tta caa atg        624

Val Ser Trp Ile Thr Phe Leu Leu Ala Lys Gly Glu Val Leu Gln Met           

        195                 200                 205                       



gaa gat gat ctg gtg att tca ttt cag tta atg cta tgt gtc ctt gac        672

Glu Asp Asp Leu Val Ile Ser Phe Gln Leu Met Leu Cys Val Leu Asp           

    210                 215                 220                           



tat ttt att aaa ctc tca cct ccc atg ttg ctc aaa gaa cca tat aaa        720

Tyr Phe Ile Lys Leu Ser Pro Pro Met Leu Leu Lys Glu Pro Tyr Lys           

225                 230                 235                 240           



aca gct gtt ata ccc att aat ggt tca cct cga aca ccc agg cga ggt        768

Thr Ala Val Ile Pro Ile Asn Gly Ser Pro Arg Thr Pro Arg Arg Gly           

                245                 250                 255               



cag aac agg agt gca cgg ata gca aaa caa cta gaa aat gat aca aga        816

Gln Asn Arg Ser Ala Arg Ile Ala Lys Gln Leu Glu Asn Asp Thr Arg           

            260                 265                 270                   



att att gaa gtt ctc tgt aaa gaa cat gaa tgt aat ata gat gag gtg        864

Ile Ile Glu Val Leu Cys Lys Glu His Glu Cys Asn Ile Asp Glu Val           

        275                 280                 285                       



aaa aat gtt tat ttc aaa aat ttt ata cct ttt atg aat tct ctt gga        912

Lys Asn Val Tyr Phe Lys Asn Phe Ile Pro Phe Met Asn Ser Leu Gly           

    290                 295                 300                           



ctt gta aca tct aat gga ctt cca gag gtt gaa aat ctt tct aaa cga        960

Leu Val Thr Ser Asn Gly Leu Pro Glu Val Glu Asn Leu Ser Lys Arg           

305                 310                 315                 320           



tac gaa gaa att tat ctt aaa aat aaa gat cta gat gca aga tta ttt       1008

Tyr Glu Glu Ile Tyr Leu Lys Asn Lys Asp Leu Asp Ala Arg Leu Phe           

                325                 330                 335               



ttg gat cat gat aaa act ctt cag act gat tct ata gac agt ttt gaa       1056

Leu Asp His Asp Lys Thr Leu Gln Thr Asp Ser Ile Asp Ser Phe Glu           

            340                 345                 350                   



aca cag aga aca cca cga aaa agt aac ctt gat gaa gag gtg aat gta       1104

Thr Gln Arg Thr Pro Arg Lys Ser Asn Leu Asp Glu Glu Val Asn Val           

        355                 360                 365                       



att cct cca cac act cca gtt agg act gtt atg aac act atc caa caa       1152

Ile Pro Pro His Thr Pro Val Arg Thr Val Met Asn Thr Ile Gln Gln           

    370                 375                 380                           



tta atg atg att tta aat tca gca agt gat caa cct tca gaa aat ctg       1200

Leu Met Met Ile Leu Asn Ser Ala Ser Asp Gln Pro Ser Glu Asn Leu           

385                 390                 395                 400           



att tcc tat ttt aac aac tgc aca gtg aat cca aaa gaa agt ata ctg       1248

Ile Ser Tyr Phe Asn Asn Cys Thr Val Asn Pro Lys Glu Ser Ile Leu           

                405                 410                 415               



aaa aga gtg aag gat ata gga tac atc ttt aaa gag aaa ttt gct aaa       1296

Lys Arg Val Lys Asp Ile Gly Tyr Ile Phe Lys Glu Lys Phe Ala Lys           

            420                 425                 430                   



gct gtg gga cag ggt tgt gtc gaa att gga tca cag cga tac aaa ctt       1344

Ala Val Gly Gln Gly Cys Val Glu Ile Gly Ser Gln Arg Tyr Lys Leu           

        435                 440                 445                       



gga gtt cgc ttg tat tac cga gta atg gaa tcc atg ctt aaa tca gaa       1392

Gly Val Arg Leu Tyr Tyr Arg Val Met Glu Ser Met Leu Lys Ser Glu           

    450                 455                 460                           



gaa gaa cga tta tcc att caa aat ttt agc aaa ctt ctg aat gac aac       1440

Glu Glu Arg Leu Ser Ile Gln Asn Phe Ser Lys Leu Leu Asn Asp Asn           

465                 470                 475                 480           



att ttt cat atg tct tta ttg gcg tgc gct ctt gag gtt gta atg gcc       1488

Ile Phe His Met Ser Leu Leu Ala Cys Ala Leu Glu Val Val Met Ala           

                485                 490                 495               



aca tat agc aga agt aca tct cag aat ctt gat tct gga aca gat ttg       1536

Thr Tyr Ser Arg Ser Thr Ser Gln Asn Leu Asp Ser Gly Thr Asp Leu           

            500                 505                 510                   



tct ttc cca tgg att ctg aat gtg ctt aat tta aaa gcc ttt gat ttt       1584

Ser Phe Pro Trp Ile Leu Asn Val Leu Asn Leu Lys Ala Phe Asp Phe           

        515                 520                 525                       



tac aaa gtg atc gaa agt ttt atc aaa gca gaa ggc aac ttg aca aga       1632

Tyr Lys Val Ile Glu Ser Phe Ile Lys Ala Glu Gly Asn Leu Thr Arg           

    530                 535                 540                           



gaa atg ata aaa cat tta gaa cga tgt gaa cat cga atc atg gaa tcc       1680

Glu Met Ile Lys His Leu Glu Arg Cys Glu His Arg Ile Met Glu Ser           

545                 550                 555                 560           



ctt gca tgg ctc tca gat tca cct tta ttt gat ctt att aaa caa tca       1728

Leu Ala Trp Leu Ser Asp Ser Pro Leu Phe Asp Leu Ile Lys Gln Ser           

                565                 570                 575               



aag gac cga gaa gga cca act gat cac ctt gaa tct gct tgt cct ctt       1776

Lys Asp Arg Glu Gly Pro Thr Asp His Leu Glu Ser Ala Cys Pro Leu           

            580                 585                 590                   



aat ctt cct ctc cag aat aat cac act gca gca gat atg tat ctt tct       1824

Asn Leu Pro Leu Gln Asn Asn His Thr Ala Ala Asp Met Tyr Leu Ser           

        595                 600                 605                       



cct gta aga tct cca aag aaa aaa ggt tca act acg cgt gta aat tct       1872

Pro Val Arg Ser Pro Lys Lys Lys Gly Ser Thr Thr Arg Val Asn Ser           

    610                 615                 620                           



act gca aat gca gag aca caa gca acc tca gcc ttc cag acc cag aag       1920

Thr Ala Asn Ala Glu Thr Gln Ala Thr Ser Ala Phe Gln Thr Gln Lys           

625                 630                 635                 640           



cca ttg aaa tct acc tct ctt tca ctg ttt tat aaa aaa gtg tat cgg       1968

Pro Leu Lys Ser Thr Ser Leu Ser Leu Phe Tyr Lys Lys Val Tyr Arg           

                645                 650                 655               



cta gcc tat ctc cgg cta aat aca ctt tgt gaa cgc ctt ctg tct gag       2016

Leu Ala Tyr Leu Arg Leu Asn Thr Leu Cys Glu Arg Leu Leu Ser Glu           

            660                 665                 670                   



cac cca gaa tta gaa cat atc atc tgg acc ctt ttc cag cac acc ctg       2064

His Pro Glu Leu Glu His Ile Ile Trp Thr Leu Phe Gln His Thr Leu           

        675                 680                 685                       



cag aat gag tat gaa ctc atg aga gac agg cat ttg gac caa att atg       2112

Gln Asn Glu Tyr Glu Leu Met Arg Asp Arg His Leu Asp Gln Ile Met           

    690                 695                 700                           



atg tgt tcc atg tat ggc ata tgc aaa gtg aag aat ata gac ctt aaa       2160

Met Cys Ser Met Tyr Gly Ile Cys Lys Val Lys Asn Ile Asp Leu Lys           

705                 710                 715                 720           



ttc aaa atc att gta aca gca tac aag gat ctt cct cat gct gtt cag       2208

Phe Lys Ile Ile Val Thr Ala Tyr Lys Asp Leu Pro His Ala Val Gln           

                725                 730                 735               



gag aca ttc aaa cgt gtt ttg atc aaa gaa gag gag tat gat tct att       2256

Glu Thr Phe Lys Arg Val Leu Ile Lys Glu Glu Glu Tyr Asp Ser Ile           

            740                 745                 750                   



ata gta ttc tat aac tcg gtc ttc atg cag aga ctg aaa aca aat att       2304

Ile Val Phe Tyr Asn Ser Val Phe Met Gln Arg Leu Lys Thr Asn Ile           

        755                 760                 765                       



ttg cag tat gct tcc acc agg ccc cct acc ttg tca cca ata cct cac       2352

Leu Gln Tyr Ala Ser Thr Arg Pro Pro Thr Leu Ser Pro Ile Pro His           

    770                 775                 780                           



att cct cga agc cct tac aag ttt cct agt tca ccc tta cgg att cct       2400

Ile Pro Arg Ser Pro Tyr Lys Phe Pro Ser Ser Pro Leu Arg Ile Pro           

785                 790                 795                 800           



gga ggg aac atc tat att tca ccc ctg aag agt cca tat aaa att tca       2448

Gly Gly Asn Ile Tyr Ile Ser Pro Leu Lys Ser Pro Tyr Lys Ile Ser           

                805                 810                 815               



gaa ggt ctg cca aca cca aca aaa atg act cca aga tca aga atc tta       2496

Glu Gly Leu Pro Thr Pro Thr Lys Met Thr Pro Arg Ser Arg Ile Leu           

            820                 825                 830                   



gta tca att ggt gaa tca ttc ggg act tct gag aag ttc cag aaa ata       2544

Val Ser Ile Gly Glu Ser Phe Gly Thr Ser Glu Lys Phe Gln Lys Ile           

        835                 840                 845                       



aat cag atg gta tgt aac agc gac cgt gtg ctc aaa aga agt gct gaa       2592

Asn Gln Met Val Cys Asn Ser Asp Arg Val Leu Lys Arg Ser Ala Glu           

    850                 855                 860                           



gga agc aac cct cct aaa cca ctg aaa aaa cta cgc ttt gat att gaa       2640

Gly Ser Asn Pro Pro Lys Pro Leu Lys Lys Leu Arg Phe Asp Ile Glu           

865                 870                 875                 880           



gga tca gat gaa gca gat gga agt aaa cat ctc cca gga gag tcc aaa       2688

Gly Ser Asp Glu Ala Asp Gly Ser Lys His Leu Pro Gly Glu Ser Lys           

                885                 890                 895               



ttt cag cag aaa ctg gca gaa atg act tct act cga aca cga atg caa       2736

Phe Gln Gln Lys Leu Ala Glu Met Thr Ser Thr Arg Thr Arg Met Gln           

            900                 905                 910                   



aag cag aaa atg aat gat agc atg gat acc tca aac aag gaa gag aaa       2784

Lys Gln Lys Met Asn Asp Ser Met Asp Thr Ser Asn Lys Glu Glu Lys           

        915                 920                 925                       



tga                                                                   2787





<210>  57

<211>  928

<212>  PRT

<213>  Homo sapiens



<400>  57



Met Pro Pro Lys Thr Pro Arg Lys Thr Ala Ala Thr Ala Ala Ala Ala 

1               5                   10                  15      





Ala Ala Glu Pro Pro Ala Pro Pro Pro Pro Pro Pro Pro Glu Glu Asp 

            20                  25                  30          





Pro Glu Gln Asp Ser Gly Pro Glu Asp Leu Pro Leu Val Arg Leu Glu 

        35                  40                  45              





Phe Glu Glu Thr Glu Glu Pro Asp Phe Thr Ala Leu Cys Gln Lys Leu 

    50                  55                  60                  





Lys Ile Pro Asp His Val Arg Glu Arg Ala Trp Leu Thr Trp Glu Lys 

65                  70                  75                  80  





Val Ser Ser Val Asp Gly Val Leu Gly Gly Tyr Ile Gln Lys Lys Lys 

                85                  90                  95      





Glu Leu Trp Gly Ile Cys Ile Phe Ile Ala Ala Val Asp Leu Asp Glu 

            100                 105                 110         





Met Ser Phe Thr Phe Thr Glu Leu Gln Lys Asn Ile Glu Ile Ser Val 

        115                 120                 125             





His Lys Phe Phe Asn Leu Leu Lys Glu Ile Asp Thr Ser Thr Lys Val 

    130                 135                 140                 





Asp Asn Ala Met Ser Arg Leu Leu Lys Lys Tyr Asp Val Leu Phe Ala 

145                 150                 155                 160 





Leu Phe Ser Lys Leu Glu Arg Thr Cys Glu Leu Ile Tyr Leu Thr Gln 

                165                 170                 175     





Pro Ser Ser Ser Ile Ser Thr Glu Ile Asn Ser Ala Leu Val Leu Lys 

            180                 185                 190         





Val Ser Trp Ile Thr Phe Leu Leu Ala Lys Gly Glu Val Leu Gln Met 

        195                 200                 205             





Glu Asp Asp Leu Val Ile Ser Phe Gln Leu Met Leu Cys Val Leu Asp 

    210                 215                 220                 





Tyr Phe Ile Lys Leu Ser Pro Pro Met Leu Leu Lys Glu Pro Tyr Lys 

225                 230                 235                 240 





Thr Ala Val Ile Pro Ile Asn Gly Ser Pro Arg Thr Pro Arg Arg Gly 

                245                 250                 255     





Gln Asn Arg Ser Ala Arg Ile Ala Lys Gln Leu Glu Asn Asp Thr Arg 

            260                 265                 270         





Ile Ile Glu Val Leu Cys Lys Glu His Glu Cys Asn Ile Asp Glu Val 

        275                 280                 285             





Lys Asn Val Tyr Phe Lys Asn Phe Ile Pro Phe Met Asn Ser Leu Gly 

    290                 295                 300                 





Leu Val Thr Ser Asn Gly Leu Pro Glu Val Glu Asn Leu Ser Lys Arg 

305                 310                 315                 320 





Tyr Glu Glu Ile Tyr Leu Lys Asn Lys Asp Leu Asp Ala Arg Leu Phe 

                325                 330                 335     





Leu Asp His Asp Lys Thr Leu Gln Thr Asp Ser Ile Asp Ser Phe Glu 

            340                 345                 350         





Thr Gln Arg Thr Pro Arg Lys Ser Asn Leu Asp Glu Glu Val Asn Val 

        355                 360                 365             





Ile Pro Pro His Thr Pro Val Arg Thr Val Met Asn Thr Ile Gln Gln 

    370                 375                 380                 





Leu Met Met Ile Leu Asn Ser Ala Ser Asp Gln Pro Ser Glu Asn Leu 

385                 390                 395                 400 





Ile Ser Tyr Phe Asn Asn Cys Thr Val Asn Pro Lys Glu Ser Ile Leu 

                405                 410                 415     





Lys Arg Val Lys Asp Ile Gly Tyr Ile Phe Lys Glu Lys Phe Ala Lys 

            420                 425                 430         





Ala Val Gly Gln Gly Cys Val Glu Ile Gly Ser Gln Arg Tyr Lys Leu 

        435                 440                 445             





Gly Val Arg Leu Tyr Tyr Arg Val Met Glu Ser Met Leu Lys Ser Glu 

    450                 455                 460                 





Glu Glu Arg Leu Ser Ile Gln Asn Phe Ser Lys Leu Leu Asn Asp Asn 

465                 470                 475                 480 





Ile Phe His Met Ser Leu Leu Ala Cys Ala Leu Glu Val Val Met Ala 

                485                 490                 495     





Thr Tyr Ser Arg Ser Thr Ser Gln Asn Leu Asp Ser Gly Thr Asp Leu 

            500                 505                 510         





Ser Phe Pro Trp Ile Leu Asn Val Leu Asn Leu Lys Ala Phe Asp Phe 

        515                 520                 525             





Tyr Lys Val Ile Glu Ser Phe Ile Lys Ala Glu Gly Asn Leu Thr Arg 

    530                 535                 540                 





Glu Met Ile Lys His Leu Glu Arg Cys Glu His Arg Ile Met Glu Ser 

545                 550                 555                 560 





Leu Ala Trp Leu Ser Asp Ser Pro Leu Phe Asp Leu Ile Lys Gln Ser 

                565                 570                 575     





Lys Asp Arg Glu Gly Pro Thr Asp His Leu Glu Ser Ala Cys Pro Leu 

            580                 585                 590         





Asn Leu Pro Leu Gln Asn Asn His Thr Ala Ala Asp Met Tyr Leu Ser 

        595                 600                 605             





Pro Val Arg Ser Pro Lys Lys Lys Gly Ser Thr Thr Arg Val Asn Ser 

    610                 615                 620                 





Thr Ala Asn Ala Glu Thr Gln Ala Thr Ser Ala Phe Gln Thr Gln Lys 

625                 630                 635                 640 





Pro Leu Lys Ser Thr Ser Leu Ser Leu Phe Tyr Lys Lys Val Tyr Arg 

                645                 650                 655     





Leu Ala Tyr Leu Arg Leu Asn Thr Leu Cys Glu Arg Leu Leu Ser Glu 

            660                 665                 670         





His Pro Glu Leu Glu His Ile Ile Trp Thr Leu Phe Gln His Thr Leu 

        675                 680                 685             





Gln Asn Glu Tyr Glu Leu Met Arg Asp Arg His Leu Asp Gln Ile Met 

    690                 695                 700                 





Met Cys Ser Met Tyr Gly Ile Cys Lys Val Lys Asn Ile Asp Leu Lys 

705                 710                 715                 720 





Phe Lys Ile Ile Val Thr Ala Tyr Lys Asp Leu Pro His Ala Val Gln 

                725                 730                 735     





Glu Thr Phe Lys Arg Val Leu Ile Lys Glu Glu Glu Tyr Asp Ser Ile 

            740                 745                 750         





Ile Val Phe Tyr Asn Ser Val Phe Met Gln Arg Leu Lys Thr Asn Ile 

        755                 760                 765             





Leu Gln Tyr Ala Ser Thr Arg Pro Pro Thr Leu Ser Pro Ile Pro His 

    770                 775                 780                 





Ile Pro Arg Ser Pro Tyr Lys Phe Pro Ser Ser Pro Leu Arg Ile Pro 

785                 790                 795                 800 





Gly Gly Asn Ile Tyr Ile Ser Pro Leu Lys Ser Pro Tyr Lys Ile Ser 

                805                 810                 815     





Glu Gly Leu Pro Thr Pro Thr Lys Met Thr Pro Arg Ser Arg Ile Leu 

            820                 825                 830         





Val Ser Ile Gly Glu Ser Phe Gly Thr Ser Glu Lys Phe Gln Lys Ile 

        835                 840                 845             





Asn Gln Met Val Cys Asn Ser Asp Arg Val Leu Lys Arg Ser Ala Glu 

    850                 855                 860                 





Gly Ser Asn Pro Pro Lys Pro Leu Lys Lys Leu Arg Phe Asp Ile Glu 

865                 870                 875                 880 





Gly Ser Asp Glu Ala Asp Gly Ser Lys His Leu Pro Gly Glu Ser Lys 

                885                 890                 895     





Phe Gln Gln Lys Leu Ala Glu Met Thr Ser Thr Arg Thr Arg Met Gln 

            900                 905                 910         





Lys Gln Lys Met Asn Asp Ser Met Asp Thr Ser Asn Lys Glu Glu Lys 

        915                 920                 925             





<210>  58

<211>  921

<212>  PRT

<213>  Mus musculus





<220>

<221>  PEPTIDE

<222>  (1)..(921)

<223>  RB_MOUSE Retinoblastoma-associated protein OS=Mus musculus GN=Rb1

       PE=1 SV=2 (P13405)



<400>  58



Met Pro Pro Lys Ala Pro Arg Arg Ala Ala Ala Ala Glu Pro Pro Pro 

1               5                   10                  15      





Pro Pro Pro Pro Pro Pro Arg Glu Asp Asp Pro Ala Gln Asp Ser Gly 

            20                  25                  30          





Pro Glu Glu Leu Pro Leu Ala Arg Leu Glu Phe Glu Glu Ile Glu Glu 

        35                  40                  45              





Pro Glu Phe Ile Ala Leu Cys Gln Lys Leu Lys Val Pro Asp His Val 

    50                  55                  60                  





Arg Glu Arg Ala Trp Leu Thr Trp Glu Lys Val Ser Ser Val Asp Gly 

65                  70                  75                  80  





Ile Leu Glu Gly Tyr Ile Gln Lys Lys Lys Glu Leu Trp Gly Ile Cys 

                85                  90                  95      





Ile Phe Ile Ala Ala Val Asp Leu Asp Glu Met Pro Phe Thr Phe Thr 

            100                 105                 110         





Glu Leu Gln Lys Ser Ile Glu Thr Ser Val Tyr Lys Phe Phe Asp Leu 

        115                 120                 125             





Leu Lys Glu Ile Asp Thr Ser Thr Lys Val Asp Asn Ala Met Ser Arg 

    130                 135                 140                 





Leu Leu Lys Lys Tyr Asn Val Leu Cys Ala Leu Tyr Ser Lys Leu Glu 

145                 150                 155                 160 





Arg Thr Cys Glu Leu Ile Tyr Leu Thr Gln Pro Ser Ser Ala Leu Ser 

                165                 170                 175     





Thr Glu Ile Asn Ser Met Leu Val Leu Lys Ile Ser Trp Ile Thr Phe 

            180                 185                 190         





Leu Leu Ala Lys Gly Glu Val Leu Gln Met Glu Asp Asp Leu Val Ile 

        195                 200                 205             





Ser Phe Gln Leu Met Leu Cys Val Val Asp Tyr Phe Ile Lys Phe Ser 

    210                 215                 220                 





Pro Pro Ala Leu Leu Arg Glu Pro Tyr Lys Thr Ala Ala Ile Pro Ile 

225                 230                 235                 240 





Asn Gly Ser Pro Arg Thr Pro Arg Arg Gly Gln Asn Arg Ser Ala Arg 

                245                 250                 255     





Ile Ala Lys Gln Leu Glu Asn Asp Thr Arg Ile Ile Glu Val Leu Cys 

            260                 265                 270         





Lys Glu His Glu Cys Asn Ile Asp Glu Val Lys Asn Val Tyr Phe Lys 

        275                 280                 285             





Asn Phe Ile Pro Phe Ile Asn Ser Leu Gly Ile Val Ser Ser Asn Gly 

    290                 295                 300                 





Leu Pro Glu Val Glu Ser Leu Ser Lys Arg Tyr Glu Glu Val Tyr Leu 

305                 310                 315                 320 





Lys Asn Lys Asp Leu Asp Ala Arg Leu Phe Leu Asp His Asp Lys Thr 

                325                 330                 335     





Leu Gln Thr Asp Pro Ile Asp Ser Phe Glu Thr Glu Arg Thr Pro Arg 

            340                 345                 350         





Lys Asn Asn Pro Asp Glu Glu Ala Asn Val Val Thr Pro His Thr Pro 

        355                 360                 365             





Val Arg Thr Val Met Asn Thr Ile Gln Gln Leu Met Val Ile Leu Asn 

    370                 375                 380                 





Ser Ala Ser Asp Gln Pro Ser Glu Asn Leu Ile Ser Tyr Phe Asn Asn 

385                 390                 395                 400 





Cys Thr Val Asn Pro Lys Glu Asn Ile Leu Lys Arg Val Lys Asp Val 

                405                 410                 415     





Gly His Ile Phe Lys Glu Lys Phe Ala Asn Ala Val Gly Gln Gly Cys 

            420                 425                 430         





Val Asp Ile Gly Val Gln Arg Tyr Lys Leu Gly Val Arg Leu Tyr Tyr 

        435                 440                 445             





Arg Val Met Glu Ser Met Leu Lys Ser Glu Glu Glu Arg Leu Ser Ile 

    450                 455                 460                 





Gln Asn Phe Ser Lys Leu Leu Asn Asp Asn Ile Phe His Met Ser Leu 

465                 470                 475                 480 





Leu Ala Cys Ala Leu Glu Val Val Met Ala Thr Tyr Ser Arg Ser Thr 

                485                 490                 495     





Leu Gln His Leu Asp Ser Gly Thr Asp Leu Ser Phe Pro Trp Ile Leu 

            500                 505                 510         





Asn Val Leu Asn Leu Lys Ala Phe Asp Phe Tyr Lys Val Ile Glu Ser 

        515                 520                 525             





Phe Ile Lys Val Glu Ala Asn Leu Thr Arg Glu Met Ile Lys His Leu 

    530                 535                 540                 





Glu Arg Cys Glu His Arg Ile Met Glu Ser Leu Ala Trp Leu Ser Asp 

545                 550                 555                 560 





Ser Pro Leu Phe Asp Leu Ile Lys Gln Ser Lys Asp Gly Glu Gly Pro 

                565                 570                 575     





Asp Asn Leu Glu Pro Ala Cys Pro Leu Ser Leu Pro Leu Gln Gly Asn 

            580                 585                 590         





His Thr Ala Ala Asp Met Tyr Leu Ser Pro Leu Arg Ser Pro Lys Lys 

        595                 600                 605             





Arg Thr Ser Thr Thr Arg Val Asn Ser Ala Ala Asn Thr Glu Thr Gln 

    610                 615                 620                 





Ala Ala Ser Ala Phe His Thr Gln Lys Pro Leu Lys Ser Thr Ser Leu 

625                 630                 635                 640 





Ala Leu Phe Tyr Lys Lys Val Tyr Arg Leu Ala Tyr Leu Arg Leu Asn 

                645                 650                 655     





Thr Leu Cys Ala Arg Leu Leu Ser Asp His Pro Glu Leu Glu His Ile 

            660                 665                 670         





Ile Trp Thr Leu Phe Gln His Thr Leu Gln Asn Glu Tyr Glu Leu Met 

        675                 680                 685             





Arg Asp Arg His Leu Asp Gln Ile Met Met Cys Ser Met Tyr Gly Ile 

    690                 695                 700                 





Cys Lys Val Lys Asn Ile Asp Leu Lys Phe Lys Ile Ile Val Thr Ala 

705                 710                 715                 720 





Tyr Lys Asp Leu Pro His Ala Ala Gln Glu Thr Phe Lys Arg Val Leu 

                725                 730                 735     





Ile Arg Glu Glu Glu Phe Asp Ser Ile Ile Val Phe Tyr Asn Ser Val 

            740                 745                 750         





Phe Met Gln Arg Leu Lys Thr Asn Ile Leu Gln Tyr Ala Ser Thr Arg 

        755                 760                 765             





Pro Pro Thr Leu Ser Pro Ile Pro His Ile Pro Arg Ser Pro Tyr Lys 

    770                 775                 780                 





Phe Ser Ser Ser Pro Leu Arg Ile Pro Gly Gly Asn Ile Tyr Ile Ser 

785                 790                 795                 800 





Pro Leu Lys Ser Pro Tyr Lys Ile Ser Glu Gly Leu Pro Thr Pro Thr 

                805                 810                 815     





Lys Met Thr Pro Arg Ser Arg Ile Leu Val Ser Ile Gly Glu Ser Phe 

            820                 825                 830         





Gly Thr Ser Glu Lys Phe Gln Lys Ile Asn Gln Met Val Cys Asn Ser 

        835                 840                 845             





Asp Arg Val Leu Lys Arg Ser Ala Glu Gly Gly Asn Pro Pro Lys Pro 

    850                 855                 860                 





Leu Lys Lys Leu Arg Phe Asp Ile Glu Gly Ala Asp Glu Ala Asp Gly 

865                 870                 875                 880 





Ser Lys His Leu Pro Ala Glu Ser Lys Phe Gln Gln Lys Leu Ala Glu 

                885                 890                 895     





Met Thr Ser Thr Arg Thr Arg Met Gln Lys Gln Arg Met Asn Glu Ser 

            900                 905                 910         





Lys Asp Val Ser Asn Lys Glu Glu Lys 

        915                 920     





<210>  59

<211>  410

<212>  PRT

<213>  Mus musculus





<220>

<221>  peptide

<222>  (1)..(410)

<223>  TFDP1_MOUSE Transcription factor Dp-1 OS=Mus musculus GN=Tfdp1 

       PE=1 SV=1 (Q08639)



<400>  59



Met Ala Lys Asp Ala Ser Leu Ile Glu Ala Asn Gly Glu Leu Lys Val 

1               5                   10                  15      





Phe Ile Asp Gln Asn Leu Ser Pro Gly Lys Gly Val Val Ser Leu Val 

            20                  25                  30          





Ala Val His Pro Ser Thr Val Asn Thr Leu Gly Lys Gln Leu Leu Pro 

        35                  40                  45              





Lys Thr Phe Gly Gln Ser Asn Val Asn Ile Thr Gln Gln Val Val Ile 

    50                  55                  60                  





Gly Thr Pro Gln Arg Pro Ala Ala Ser Asn Thr Ile Val Val Gly Ser 

65                  70                  75                  80  





Pro His Thr Pro Asn Thr His Phe Val Ser Gln Asn Gln Thr Ser Asp 

                85                  90                  95      





Ser Ser Pro Trp Ser Ala Gly Lys Arg Asn Arg Lys Gly Glu Lys Asn 

            100                 105                 110         





Gly Lys Gly Leu Arg His Phe Ser Met Lys Val Cys Glu Lys Val Gln 

        115                 120                 125             





Arg Lys Gly Thr Thr Ser Tyr Asn Glu Val Ala Asp Glu Leu Val Ala 

    130                 135                 140                 





Glu Phe Ser Ala Ala Asp Asn His Ile Leu Pro Asn Glu Ser Ala Tyr 

145                 150                 155                 160 





Asp Gln Lys Asn Ile Arg Arg Arg Val Tyr Asp Ala Leu Asn Val Leu 

                165                 170                 175     





Met Ala Met Asn Ile Ile Ser Lys Glu Lys Lys Glu Ile Lys Trp Ile 

            180                 185                 190         





Gly Leu Pro Thr Asn Ser Ala Gln Glu Cys Gln Asn Leu Glu Val Glu 

        195                 200                 205             





Arg Gln Arg Arg Leu Glu Arg Ile Lys Gln Lys Gln Ser Gln Leu Gln 

    210                 215                 220                 





Glu Leu Ile Leu Gln Gln Ile Ala Phe Lys Asn Leu Val Gln Arg Asn 

225                 230                 235                 240 





Arg Gln Ala Glu Gln Gln Ala Arg Arg Pro Pro Pro Pro Asn Ser Val 

                245                 250                 255     





Ile His Leu Pro Phe Ile Ile Val Asn Thr Ser Arg Lys Thr Val Ile 

            260                 265                 270         





Asp Cys Ser Ile Ser Asn Asp Lys Phe Glu Tyr Leu Phe Asn Phe Asp 

        275                 280                 285             





Asn Thr Phe Glu Ile His Asp Asp Ile Glu Val Leu Lys Arg Met Gly 

    290                 295                 300                 





Met Ala Cys Gly Leu Glu Ser Gly Asn Cys Ser Ala Glu Asp Leu Lys 

305                 310                 315                 320 





Val Ala Arg Ser Leu Val Pro Lys Ala Leu Glu Pro Tyr Val Thr Glu 

                325                 330                 335     





Met Ala Gln Gly Ser Ile Gly Gly Val Phe Val Thr Thr Thr Gly Ser 

            340                 345                 350         





Thr Ser Asn Gly Thr Arg Leu Ser Ala Ser Asp Leu Ser Asn Gly Ala 

        355                 360                 365             





Asp Gly Met Leu Ala Thr Ser Ser Asn Gly Ser Gln Tyr Ser Gly Ser 

    370                 375                 380                 





Arg Val Glu Thr Pro Val Ser Tyr Val Gly Glu Asp Asp Asp Asp Asp 

385                 390                 395                 400 





Asp Asp Phe Asn Glu Asn Asp Glu Glu Asp 

                405                 410 





