                         SEQUENCE LISTING

<110>  Georgia Sate University Research Foundation, Inc.
 
<120>  CALCIUM SENSING RECEPTORS, LIGANDS, COMPOSITIONS, AND METHODS OF 
       USE

<130>  220702-2270

<150>  US 62/314,707
<151>  2016-03-29

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  593
<212>  PRT
<213>  Homo sapiens

<400>  1

Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile Ile Leu Gly 
1               5                   10                  15      


Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp Gln Asp Leu 
            20                  25                  30          


Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn Phe Arg Gly 
        35                  40                  45              


Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu Ile Asn Ser 
    50                  55                  60                  


Ser Pro Ala Leu Leu Pro Asn Leu Thr Leu Gly Tyr Arg Ile Phe Asp 
65                  70                  75                  80  


Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu Ser Phe Val 
                85                  90                  95      


Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe Cys Asn Cys 
            100                 105                 110         


Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala Thr Gly Ser 
        115                 120                 125             


Gly Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe Tyr Ile Pro 
    130                 135                 140                 


Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn Lys Asn Gln 
145                 150                 155                 160 


Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His Gln Ala Thr 
                165                 170                 175     


Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp Val Gly Thr 
            180                 185                 190         


Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu Lys Phe Arg 
        195                 200                 205             


Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser Glu Leu Ile 
    210                 215                 220                 


Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln His Val Val Glu Val Ile 
225                 230                 235                 240 


Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser Gly Pro Asp 
                245                 250                 255     


Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile Thr Gly Lys 
            260                 265                 270         


Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu Ile Ala Met 
        275                 280                 285             


Pro Gln Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe Ala Leu Lys 
    290                 295                 300                 


Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Lys Lys Val His Pro 
305                 310                 315                 320 


Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp Glu Glu Thr 
                325                 330                 335     


Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu Pro Val Asp 
            340                 345                 350         


Thr Phe Leu Arg Gly His Glu Glu Ser Gly Asp Arg Phe Ser Asn Ser 
        355                 360                 365             


Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn Ile Ser Ser 
    370                 375                 380                 


Val Glu Thr Pro Tyr Ile Asp Tyr Thr His Leu Arg Ile Ser Tyr Asn 
385                 390                 395                 400 


Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln Asp Ile Tyr 
                405                 410                 415     


Thr Cys Leu Pro Gly Arg Gly Leu Phe Thr Asn Gly Ser Cys Ala Asp 
            420                 425                 430         


Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu Arg His Leu 
        435                 440                 445             


Asn Phe Thr Asn Asn Met Gly Glu Gln Val Thr Phe Asp Glu Cys Gly 
    450                 455                 460                 


Asp Leu Val Gly Asn Tyr Ser Ile Ile Asn Trp His Leu Ser Pro Glu 
465                 470                 475                 480 


Asp Gly Ser Ile Val Phe Lys Glu Val Gly Tyr Tyr Asn Val Tyr Ala 
                485                 490                 495     


Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Glu Lys Ile Leu Trp Ser 
            500                 505                 510         


Gly Phe Ser Arg Glu Val Pro Phe Ser Asn Cys Ser Arg Asp Cys Leu 
        515                 520                 525             


Ala Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr Cys Cys Phe 
    530                 535                 540                 


Glu Cys Val Glu Cys Pro Asp Gly Glu Tyr Ser Asp Glu Thr Asp Ala 
545                 550                 555                 560 


Ser Ala Cys Asn Lys Cys Pro Asp Asp Phe Trp Ser Asn Glu Asn His 
                565                 570                 575     


Thr Ser Cys Ile Ala Lys Glu Ile Glu Phe Leu Ser Trp Thr Glu Pro 
            580                 585                 590         


Phe 
    


<210>  2
<211>  600
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human CaSR with FLAG Tag

<400>  2

Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile Ile Leu Gly 
1               5                   10                  15      


Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp Gln Asp Leu 
            20                  25                  30          


Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn Phe Arg Gly 
        35                  40                  45              


Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu Ile Asn Ser 
    50                  55                  60                  


Ser Pro Ala Leu Leu Pro Asn Leu Thr Leu Gly Tyr Arg Ile Phe Asp 
65                  70                  75                  80  


Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu Ser Phe Val 
                85                  90                  95      


Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe Cys Asn Cys 
            100                 105                 110         


Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala Thr Gly Ser 
        115                 120                 125             


Gly Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe Tyr Ile Pro 
    130                 135                 140                 


Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn Lys Asn Gln 
145                 150                 155                 160 


Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His Gln Ala Thr 
                165                 170                 175     


Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp Val Gly Thr 
            180                 185                 190         


Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu Lys Phe Arg 
        195                 200                 205             


Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser Glu Leu Ile 
    210                 215                 220                 


Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln His Val Val Glu Val Ile 
225                 230                 235                 240 


Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser Gly Pro Asp 
                245                 250                 255     


Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile Thr Gly Lys 
            260                 265                 270         


Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu Ile Ala Met 
        275                 280                 285             


Pro Gln Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe Ala Leu Lys 
    290                 295                 300                 


Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Lys Lys Val His Pro 
305                 310                 315                 320 


Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp Glu Glu Thr 
                325                 330                 335     


Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu Pro Val Asp 
            340                 345                 350         


Tyr Lys Asp Asp Asp Asp Lys Thr Phe Leu Arg Gly His Glu Glu Ser 
        355                 360                 365             


Gly Asp Arg Phe Ser Asn Ser Ser Thr Ala Phe Arg Pro Leu Cys Thr 
    370                 375                 380                 


Gly Asp Glu Asn Ile Ser Ser Val Glu Thr Pro Tyr Ile Asp Tyr Thr 
385                 390                 395                 400 


His Leu Arg Ile Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala 
                405                 410                 415     


His Ala Leu Gln Asp Ile Tyr Thr Cys Leu Pro Gly Arg Gly Leu Phe 
            420                 425                 430         


Thr Asn Gly Ser Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val 
        435                 440                 445             


Leu Lys His Leu Arg His Leu Asn Phe Thr Asn Asn Met Gly Glu Gln 
    450                 455                 460                 


Val Thr Phe Asp Glu Cys Gly Asp Leu Val Gly Asn Tyr Ser Ile Ile 
465                 470                 475                 480 


Asn Trp His Leu Ser Pro Glu Asp Gly Ser Ile Val Phe Lys Glu Val 
                485                 490                 495     


Gly Tyr Tyr Asn Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn 
            500                 505                 510         


Glu Glu Lys Ile Leu Trp Ser Gly Phe Ser Arg Glu Val Pro Phe Ser 
        515                 520                 525             


Asn Cys Ser Arg Asp Cys Leu Ala Gly Thr Arg Lys Gly Ile Ile Glu 
    530                 535                 540                 


Gly Glu Pro Thr Cys Cys Phe Glu Cys Val Glu Cys Pro Asp Gly Glu 
545                 550                 555                 560 


Tyr Ser Asp Glu Thr Asp Ala Ser Ala Cys Asn Lys Cys Pro Asp Asp 
                565                 570                 575     


Phe Trp Ser Asn Glu Asn His Thr Ser Cys Ile Ala Lys Glu Ile Glu 
            580                 585                 590         


Phe Leu Ser Trp Thr Glu Pro Phe 
        595                 600 


<210>  3
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ECD subdomain 1 of CaSR (amino acids 132-300 of SEQ ID NO: 1)

<400>  3

Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala Thr Gly Ser 
1               5                   10                  15      


Gly Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe Tyr Ile Pro 
            20                  25                  30          


Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn Lys Asn Gln 
        35                  40                  45              


Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His Gln Ala Thr 
    50                  55                  60                  


Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp Val Gly Thr 
65                  70                  75                  80  


Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu Lys Phe Arg 
                85                  90                  95      


Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser Glu Leu Ile 
            100                 105                 110         


Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln His Val Val Glu Val Ile 
        115                 120                 125             


Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser Gly Pro Asp 
    130                 135                 140                 


Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile Thr Gly Lys 
145                 150                 155                 160 


Ile Trp Leu Ala Ser Glu Ala Trp Ala 
                165                 


<210>  4
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ECD subdomain 2 of CaSR (amino acids 185-324 of SEQ ID NO: 1)

<400>  4

Arg Thr Ile Pro Asn Asp Glu His Gln Ala Thr Ala Met Ala Asp Ile 
1               5                   10                  15      


Ile Glu Tyr Phe Arg Trp Asn Trp Val Gly Thr Ile Ala Ala Asp Asp 
            20                  25                  30          


Asp Tyr Gly Arg Pro Gly Ile Glu Lys Phe Arg Glu Glu Ala Glu Glu 
        35                  40                  45              


Arg Asp Ile Cys Ile Asp Phe Ser Glu Leu Ile Ser Gln Tyr Ser Asp 
    50                  55                  60                  


Glu Glu Glu Ile Gln His Val Val Glu Val Ile Gln Asn Ser Thr Ala 
65                  70                  75                  80  


Lys Val Ile Val Val Phe Ser Ser Gly Pro Asp Leu Glu Pro Leu Ile 
                85                  90                  95      


Lys Glu Ile Val Arg Arg Asn Ile Thr Gly Lys Ile Trp Leu Ala Ser 
            100                 105                 110         


Glu Ala Trp Ala Ser Ser Ser Leu Ile Ala Met Pro Gln Tyr Phe His 
        115                 120                 125             


Val Val Gly Gly Thr Ile Gly Phe Ala Leu Lys Ala 
    130                 135                 140 


<210>  5
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ECD subdomain 3 of human CaSR (amino acids 340-445 of SEQ ID NO: 
       1)

<400>  5

Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp Glu Glu Thr 
1               5                   10                  15      


Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu Pro Val Asp 
            20                  25                  30          


Thr Phe Leu Arg Gly His Glu Glu Ser Gly Asp Arg Phe Ser Asn Ser 
        35                  40                  45              


Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn Ile Ser Ser 
    50                  55                  60                  


Val Glu Thr Pro Tyr Ile Asp Tyr Thr His Leu Arg Ile Ser Tyr Asn 
65                  70                  75                  80  


Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln Asp Ile Tyr 
                85                  90                  95      


Thr Cys Leu Pro Gly Arg Gly Leu Phe Thr 
            100                 105     


<210>  6
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human CaSR systeine rich domain

<400>  6

Leu Trp Ser Gly Phe Ser Arg Glu Val Pro Phe Ser Asn Cys Ser Arg 
1               5                   10                  15      


Asp Cys Leu Ala Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr 
            20                  25                  30          


Cys Cys Phe Glu Cys Val Glu Cys Pro Asp Gly Glu Tyr Ser Asp Glu 
        35                  40                  45              


Thr Asp Ala Ser Ala Cys Asn Lys Cys Pro Asp Asp Phe Trp Ser Asn 
    50                  55                  60                  


Glu Asn His Thr Ser Cys Ile Ala Lys Glu Ile Glu Phe Leu Ser Trp 
65                  70                  75                  80  


Thr Glu Pro Phe 
                


<210>  7
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Shark CaSR ECD

<400>  7

Met Val Phe Pro Ser Pro Phe Gly Pro Cys Cys Gln Leu Val Leu Arg 
1               5                   10                  15      


Trp Lys Thr Ser Lys Glu Asn Arg Cys Ser Ser Leu Lys Val Ser Thr 
            20                  25                  30          


Thr Glu Ser Ala Pro Thr Ile Thr Glu Thr Ser Lys Lys Ala Gln Thr 
        35                  40                  45              


Phe Val Ser Lys Leu Thr Leu Val Glu Ala Glu Gln Ser Asp Leu Leu 
    50                  55                  60                  


Lys Ala Leu Gly Glu Glu Asp Cys Thr Met Gly Gly His His Tyr Gly 
65                  70                  75                  80  


Leu Leu Ile Leu Gly Phe Thr Leu Leu Gln Ser Tyr Cys Val Ser Glu 
                85                  90                  95      


Tyr Gly Pro Asn Gln Arg Ala Gln Lys Lys Gly Asp Ile Ile Leu Gly 
            100                 105                 110         


Gly Leu Phe Pro Ile His Phe Gly Val Thr Ala Lys Asp Gln Asp Leu 
        115                 120                 125             


Lys Ser Arg Pro Glu Met Thr Lys Cys Phe Arg Tyr Asn Phe Arg Gly 
    130                 135                 140                 


Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu Ile Asn Asn 
145                 150                 155                 160 


Ser Met Ala Phe Leu Pro Asn Ile Thr Leu Gly Tyr Arg Ile Phe Asp 
                165                 170                 175     


Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu Ser Phe Val 
            180                 185                 190         


Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe Cys Asn Cys 
        195                 200                 205             


Ser Asp His Ile Pro Ser Thr Ile Ala Val Val Gly Ala Thr Gly Ser 
    210                 215                 220                 


Gly Ile Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe Tyr Ile Pro 
225                 230                 235                 240 


Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn Lys Asn Gln 
                245                 250                 255     


Tyr Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu Gln Gln Ala Thr 
            260                 265                 270         


Ala Met Ala Asp Ile Ile Gln His Phe Gln Trp Asn Trp Val Gly Thr 
        275                 280                 285             


Leu Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu Lys Phe Arg 
    290                 295                 300                 


Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser Glu Leu Ile 
305                 310                 315                 320 


Ser Gln Tyr Tyr Thr Gln Glu Glu Leu Gln His Val Ala Glu Val Ile 
                325                 330                 335     


Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser Gly Pro Asp 
            340                 345                 350         


Leu Glu Pro Ile Ile Gln Glu Ile Val Arg Arg Asn Ile Thr Gly Arg 
        355                 360                 365             


Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu Ile Ala Lys 
    370                 375                 380                 


Pro Glu Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe Ala Leu Arg 
385                 390                 395                 400 


Ala Gly His Ile Pro Gly Phe Tyr Glu Phe Leu Gln Arg Val His Pro 
                405                 410                 415     


Ser Arg Ser Ser Asp Asn Gly Phe Val Lys Glu Phe Trp Glu Glu Thr 
            420                 425                 430         


Phe Lys Cys Tyr Leu Thr Glu Lys Ser Leu Thr Gln Leu Lys Gln Ser 
        435                 440                 445             


Lys Thr Pro Gly His Asp Gly Ser Thr Val Val Gly Asn Ile Thr Ser 
    450                 455                 460                 


Ser Lys Leu Leu Pro Pro Cys Thr Gly Asp Glu Asn Ile Met Ser Val 
465                 470                 475                 480 


Glu Thr Pro Tyr Leu Asp Tyr Thr His Met Arg Ile Ser Tyr Asn Val 
                485                 490                 495     


Tyr Met Ala Val Tyr Ser Ile Ala His Ala Leu Gln Asp Ile Tyr Thr 
            500                 505                 510         


Cys Thr Pro Gly Lys Gly Ile Phe Glu Asn Gly Ser Cys Ala Asp Ile 
        515                 520                 525             


Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu Lys His Leu Lys 
    530                 535                 540                 


Phe Thr Asn Ser Met Gly Glu Gln Val Asp Phe Asp Asp Gln Gly Asp 
545                 550                 555                 560 


Leu Lys Gly Asn Tyr Thr Ile Ile Asn Trp Gln Leu Ser Ser Glu Asp 
                565                 570                 575     


Gln Ser Val Val Phe Gln Glu Val Gly Asn Tyr Asn Ala Tyr Ala Lys 
            580                 585                 590         


Pro Gly Glu Arg Leu Tyr Ile Asn Glu Ser Lys Val Leu Trp Ser Gly 
        595                 600                 605             


Phe Ser Lys Val Val Pro Phe Ser Asn Cys Thr His Asp Cys Ile Pro 
    610                 615                 620                 


Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr Cys Cys Phe Glu 
625                 630                 635                 640 


Cys Val Ser Cys Ala Glu Gly Glu Tyr Ser Asp Glu Asn Asp Ala Ser 
                645                 650                 655     


Ala Cys Thr Lys Cys Pro Asn Asp Phe Trp Ser Asn Glu Asn His Thr 
            660                 665                 670         


Tyr Cys Ile Glu Lys Glu Ile Glu Tyr Leu Ser Trp Thr Glu Pro Phe 
        675                 680                 685             


<210>  8
<211>  601
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Zebrafish CaSR ECD

<400>  8

Met Arg Phe His Leu Lys Phe Tyr Leu His Tyr Leu Val Leu Leu Gly 
1               5                   10                  15      


Ser Ser Cys Val Ile Ser Thr Tyr Gly Pro Asn Gln Arg Ala Gln Lys 
            20                  25                  30          


Thr Gly Asp Ile Leu Leu Gly Gly Leu Phe Pro Met His Phe Gly Val 
        35                  40                  45              


Ala Ser Lys Asp Gln Asp Leu Ala Ala Arg Pro Glu Ser Thr Glu Cys 
    50                  55                  60                  


Val Arg Tyr Asn Phe Arg Gly Phe Arg Trp Leu Gln Ser Met Ile Phe 
65                  70                  75                  80  


Ala Ile Glu Glu Ile Asn Asn Ser Ser Thr Leu Leu Pro Asn Ile Thr 
                85                  90                  95      


Leu Gly Tyr Arg Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu 
            100                 105                 110         


Glu Ala Ser Leu Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn 
        115                 120                 125             


Leu Asp Glu Phe Cys Asn Cys Thr Gly Asn Ile Pro Ser Thr Ile Ala 
    130                 135                 140                 


Val Val Gly Ala Ser Gly Ser Ala Val Ser Thr Ala Val Ala Asp Leu 
145                 150                 155                 160 


Leu Gly Leu Phe Tyr Ile Pro Gln Ile Ser Tyr Ala Ser Ser Ser Arg 
                165                 170                 175     


Leu Leu Ser Asn Lys Asn Gln Tyr Lys Ser Phe Met Arg Thr Ile Pro 
            180                 185                 190         


Thr Asp Glu Tyr Gln Ala Ile Ala Met Ala Ala Ile Ile Glu His Phe 
        195                 200                 205             


Gln Trp Asn Trp Val Ile Ala Ile Ala Ser Asp Asp Glu Tyr Gly Arg 
    210                 215                 220                 


Pro Gly Ile Glu Lys Phe Glu Asn Glu Met Phe His Arg Asp Ile Cys 
225                 230                 235                 240 


Ile Asp Leu Asn Val Leu Ile Ser Gln Tyr Val Asp Glu Ala Glu Ile 
                245                 250                 255     


Arg Arg Leu Ala Asp Arg Ile Gln Asn Ser Ser Ala Lys Val Ile Val 
            260                 265                 270         


Val Phe Ala Ser Gly Pro Asp Ile Glu Pro Leu Val Lys Glu Met Val 
        275                 280                 285             


Arg Arg Asn Ile Thr Asp Arg Val Trp Leu Ala Ser Glu Ala Trp Ala 
    290                 295                 300                 


Ser Ser Ser Leu Val Ala Lys Pro Glu Tyr Leu Asp Val Met Gly Gly 
305                 310                 315                 320 


Thr Ile Gly Phe Ala Leu Arg Ala Gly His Ile Pro Gly Phe Lys Asp 
                325                 330                 335     


Phe Leu Gln Gln Val His Pro Lys Lys Ser Ser His Asn Glu Phe Val 
            340                 345                 350         


Arg Glu Phe Trp Glu Glu Thr Phe Asn Cys Tyr Leu Glu Asp Ser Pro 
        355                 360                 365             


Arg Asn Ala Asp Ser Glu Asn Gly Ser Thr Ser Phe Arg Pro Leu Cys 
    370                 375                 380                 


Thr Gly Glu Glu Asp Ile Ala Ser Val Glu Thr Pro Tyr Leu Asp Tyr 
385                 390                 395                 400 


Thr His Leu Arg Ile Ser Tyr Asn Val Tyr Val Ala Val Tyr Ala Ile 
                405                 410                 415     


Ala Gln Ala Leu Gln Asp Ile Leu Thr Cys Thr Pro Gly Lys Gly Leu 
            420                 425                 430         


Phe Ser Asn Gly Ser Cys Ala Asp Ile Arg Lys Val Glu Ala Trp Gln 
        435                 440                 445             


Val Leu Lys Gln Leu Arg His Leu Asn Phe Ile Asp Ser Met Gly Glu 
    450                 455                 460                 


Arg Val Arg Phe Asp Asn Gly Ser Glu Leu Ser Ala Asn Tyr Thr Ile 
465                 470                 475                 480 


Ile Asn Trp His Arg Ser Pro Glu Asp Gly Ser Val Val Phe Lys Glu 
                485                 490                 495     


Val Gly Tyr Tyr Ser Ile His Asn Lys Asn Val Ala Lys Leu Ser Ile 
            500                 505                 510         


Asp Lys Ser Lys Ile Leu Trp Asn Gly Arg Leu Thr Glu Val Pro Phe 
        515                 520                 525             


Ser Asn Cys Ser Val Glu Cys Glu Pro Gly Thr Arg Lys Gly Ile Ile 
    530                 535                 540                 


Asp Gly Glu Pro Thr Cys Cys Phe Glu Cys Thr Glu Cys Ser Asp Gly 
545                 550                 555                 560 


Glu Tyr Ser Asp His Lys Asp Ala Ser Phe Cys Val Lys Cys Pro Asn 
                565                 570                 575     


Asn Ser Trp Ser Asn Gly Asn His Thr Ser Cys Phe Leu Lys Gln Ile 
            580                 585                 590         


Glu Phe Leu Ser Trp Thr Glu Pro Phe 
        595                 600     


<210>  9
<211>  597
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Salmon CaSR ECD

<400>  9

Met Arg Phe Tyr Leu Tyr Tyr Leu Val Leu Leu Gly Phe Ser Ser Val 
1               5                   10                  15      


Ile Ser Thr Tyr Gly Pro His Gln Arg Ala Gln Lys Thr Gly Asp Ile 
            20                  25                  30          


Leu Leu Gly Gly Leu Phe Pro Met His Phe Gly Val Thr Ser Lys Asp 
        35                  40                  45              


Gln Asp Leu Ala Ala Arg Pro Glu Ser Thr Glu Cys Val Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Asn Ser Ser Thr Leu Leu Pro Asn Ile Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Thr Asp His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Ser Gly Ser Ala Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Ile Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Phe Lys Ser Phe Met Arg Thr Ile Pro Thr Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Asp Tyr Phe Gln Trp Asn Trp 
        195                 200                 205             


Val Ile Ala Val Ala Ser Asp Asp Glu Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Glu Lys Glu Met Glu Glu Arg Asp Ile Cys Ile His Leu Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Phe Glu Glu Trp Gln Ile Gln Gly Leu Val 
                245                 250                 255     


Asp Arg Ile Glu Asn Ser Ser Ala Lys Val Ile Val Val Phe Ala Ser 
            260                 265                 270         


Gly Pro Asp Ile Glu Pro Leu Ile Lys Glu Met Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Asp Arg Ile Trp Leu Ala Ser Glu Ala Trp Ala Thr Thr Ser Leu 
    290                 295                 300                 


Ile Ala Lys Pro Glu Tyr Leu Asp Val Val Val Gly Thr Ile Gly Phe 
305                 310                 315                 320 


Ala Leu Arg Ala Gly Glu Ile Pro Gly Phe Lys Asp Phe Leu Gln Glu 
                325                 330                 335     


Val Thr Pro Lys Lys Ser Ser His Asn Glu Phe Val Arg Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys Tyr Leu Glu Asp Ser Gln Arg Leu Arg Asp 
        355                 360                 365             


Ser Glu Asn Gly Ser Thr Ser Phe Arg Pro Leu Cys Thr Gly Glu Glu 
    370                 375                 380                 


Asp Ile Met Gly Ala Glu Thr Pro Tyr Leu Asp Tyr Thr His Leu Arg 
385                 390                 395                 400 


Ile Ser Tyr Asn Val Tyr Val Ala Val His Ser Ile Ala Gln Ala Leu 
                405                 410                 415     


Gln Asp Ile Leu Thr Cys Ile Pro Gly Arg Gly Leu Phe Ser Asn Asn 
            420                 425                 430         


Ser Cys Ala Asp Ile Lys Lys Ile Glu Ala Trp Gln Val Leu Lys Gln 
        435                 440                 445             


Leu Arg His Leu Asn Phe Ser Asn Ser Met Gly Glu Lys Val His Phe 
    450                 455                 460                 


Asp Glu Asn Ala Asp Pro Ser Gly Asn Tyr Thr Ile Ile Asn Trp His 
465                 470                 475                 480 


Arg Ser Pro Glu Asp Gly Ser Val Val Phe Glu Glu Val Gly Phe Tyr 
                485                 490                 495     


Asn Met Arg Ala Lys Arg Gly Val Gln Leu Phe Ile Asp Asn Thr Lys 
            500                 505                 510         


Ile Leu Trp Asn Gly Tyr Asn Thr Glu Val Pro Phe Ser Asn Cys Ser 
        515                 520                 525             


Glu Asp Cys Glu Pro Gly Thr Arg Lys Gly Ile Ile Glu Ser Met Pro 
    530                 535                 540                 


Thr Cys Cys Phe Glu Cys Thr Glu Cys Ser Glu Gly Glu Tyr Ser Asp 
545                 550                 555                 560 


His Lys Asp Ala Ser Val Cys Thr Lys Cys Pro Asn Asp Ser Trp Ser 
                565                 570                 575     


Asn Glu Asn His Thr Ser Cys Phe Leu Lys Glu Ile Glu Phe Leu Ser 
            580                 585                 590         


Trp Thr Glu Pro Phe 
        595         


<210>  10
<211>  611
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chicken CaSR ECD

<400>  10

Met Thr Leu Tyr Ser Cys Cys Leu Ile Leu Leu Leu Phe Thr Trp Asn 
1               5                   10                  15      


Thr Ala Ala Tyr Gly Pro Asn Gln Arg Ala Gln Lys Lys Gly Asp Ile 
            20                  25                  30          


Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp 
        35                  40                  45              


Gln Asp Leu Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Asn Ser Pro Asn Leu Leu Pro Asn Met Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Thr Gly Ser Gly Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp 
        195                 200                 205             


Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln Gln Val Val 
                245                 250                 255     


Glu Val Ile Gln Asn Ser Thr Ala Arg Val Ile Val Val Phe Ser Ser 
            260                 265                 270         


Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Gly Lys Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu 
    290                 295                 300                 


Ile Ala Met Pro Glu Phe Phe Arg Val Ile Gly Ser Thr Ile Gly Phe 
305                 310                 315                 320 


Ala Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Gln Lys 
                325                 330                 335     


Val His Pro Lys Lys Ser Ala Asn Asn Gly Phe Ala Lys Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys Tyr Leu Pro Ser Glu Ser Lys Asn Ser Pro 
        355                 360                 365             


Ala Ser Ala Ser Phe His Lys Ala His Glu Glu Gly Leu Gly Ala Gly 
    370                 375                 380                 


Asn Gly Thr Ala Ala Phe Arg Pro Pro Cys Thr Gly Asp Glu Asn Ile 
385                 390                 395                 400 


Thr Ser Val Glu Thr Pro Tyr Met Asp Phe Thr His Leu Arg Ile Ser 
                405                 410                 415     


Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln Asp 
            420                 425                 430         


Ile Tyr Thr Cys Thr Pro Gly Lys Gly Leu Phe Thr Asn Gly Ser Cys 
        435                 440                 445             


Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu Arg 
    450                 455                 460                 


His Leu Asn Phe Thr Ser Asn Met Gly Glu Gln Val Asp Phe Asp Glu 
465                 470                 475                 480 


Phe Gly Asp Leu Val Gly Asn Tyr Ser Ile Ile Asn Trp His Leu Ser 
                485                 490                 495     


Pro Glu Asp Gly Ser Val Val Phe Glu Glu Val Gly His Tyr Asn Val 
            500                 505                 510         


Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Asn Lys Ile Leu 
        515                 520                 525             


Trp Ser Gly Phe Ser Lys Glu Val Pro Phe Ser Asn Cys Ser Arg Asp 
    530                 535                 540                 


Cys Leu Pro Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr Cys 
545                 550                 555                 560 


Cys Phe Glu Cys Val Asp Cys Pro Asp Gly Glu Tyr Ser Asp Glu Thr 
                565                 570                 575     


Asp Ala Ser Ala Cys Asp Lys Cys Pro Glu Asp Tyr Trp Ser Asn Glu 
            580                 585                 590         


Asn His Thr Ser Cys Ile Pro Lys Gln Ile Glu Phe Leu Ser Trp Thr 
        595                 600                 605             


Glu Pro Phe 
    610     


<210>  11
<211>  612
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CaSR ECD

<400>  11

Met Ala Trp Phe Gly Tyr Cys Leu Ala Leu Leu Ala Leu Thr Trp His 
1               5                   10                  15      


Ser Ser Ala Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile 
            20                  25                  30          


Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ser Ala Lys Asp 
        35                  40                  45              


Gln Asp Leu Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Ser Ser Pro Ala Leu Leu Pro Asn Met Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Thr Gly Ser Gly Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp 
        195                 200                 205             


Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln Gln Val Val 
                245                 250                 255     


Glu Val Ile Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser 
            260                 265                 270         


Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Gly Arg Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu 
    290                 295                 300                 


Ile Ala Met Pro Glu Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe 
305                 310                 315                 320 


Gly Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Gln Lys 
                325                 330                 335     


Val His Pro Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys His Leu Gln Asp Gly Ala Lys Gly Pro Leu 
        355                 360                 365             


Pro Val Asp Thr Phe Val Arg Ser His Glu Glu Gly Gly Asn Arg Leu 
    370                 375                 380                 


Leu Asn Ser Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn 
385                 390                 395                 400 


Ile Asn Ser Val Glu Thr Pro Tyr Met Asp Tyr Glu His Leu Arg Ile 
                405                 410                 415     


Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln 
            420                 425                 430         


Asp Ile Tyr Thr Cys Leu Pro Gly Arg Gly Leu Phe Thr Asn Gly Ser 
        435                 440                 445             


Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu 
    450                 455                 460                 


Arg His Leu Asn Phe Thr Asn Asn Met Gly Glu Gln Val Thr Phe Asp 
465                 470                 475                 480 


Glu Cys Gly Asp Leu Val Gly Asn Tyr Ser Ile Ile Asn Trp His Leu 
                485                 490                 495     


Ser Pro Glu Asp Gly Ser Ile Val Phe Lys Glu Val Gly Tyr Tyr Asn 
            500                 505                 510         


Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Gly Lys Ile 
        515                 520                 525             


Leu Trp Ser Gly Phe Ser Arg Glu Val Pro Phe Ser Asn Cys Ser Arg 
    530                 535                 540                 


Asp Cys Gln Ala Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr 
545                 550                 555                 560 


Cys Cys Phe Glu Cys Ala Glu Cys Pro Asp Gly Glu Tyr Ser Gly Glu 
                565                 570                 575     


Thr Asp Ala Ser Ala Cys Asp Lys Cys Pro Asp Asp Phe Trp Ser Asn 
            580                 585                 590         


Glu Asn His Thr Ser Cys Ile Ala Lys Glu Ile Glu Phe Leu Ala Trp 
        595                 600                 605             


Thr Glu Pro Phe 
    610         


<210>  12
<211>  612
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rat CaSR ECD

<400>  12

Met Ala Ser Tyr Ser Cys Cys Leu Ala Leu Leu Ala Leu Ala Trp His 
1               5                   10                  15      


Ser Ser Ala Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile 
            20                  25                  30          


Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp 
        35                  40                  45              


Gln Asp Leu Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Ser Ser Pro Ser Leu Leu Pro Asn Met Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Thr Gly Ser Gly Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Tyr Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp 
        195                 200                 205             


Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln Gln Val Val 
                245                 250                 255     


Glu Val Ile Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser 
            260                 265                 270         


Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Gly Arg Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu 
    290                 295                 300                 


Ile Ala Met Pro Glu Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe 
305                 310                 315                 320 


Gly Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Gln Lys 
                325                 330                 335     


Val His Pro Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu 
        355                 360                 365             


Pro Val Asp Thr Phe Val Arg Ser His Glu Glu Gly Gly Asn Arg Leu 
    370                 375                 380                 


Leu Asn Ser Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn 
385                 390                 395                 400 


Ile Asn Ser Val Glu Thr Pro Tyr Met Asp Tyr Glu His Leu Arg Ile 
                405                 410                 415     


Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln 
            420                 425                 430         


Asp Ile Tyr Thr Cys Leu Pro Gly Arg Gly Leu Phe Thr Asn Gly Ser 
        435                 440                 445             


Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu 
    450                 455                 460                 


Arg His Leu Asn Phe Thr Asn Asn Met Gly Glu Gln Val Thr Phe Asp 
465                 470                 475                 480 


Glu Cys Gly Asp Leu Val Gly Asn Tyr Ser Ile Ile Asn Trp His Leu 
                485                 490                 495     


Ser Pro Glu Asp Gly Ser Ile Val Phe Lys Glu Val Gly Tyr Tyr Asn 
            500                 505                 510         


Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Glu Lys Ile 
        515                 520                 525             


Leu Trp Ser Gly Phe Ser Arg Glu Val Pro Phe Ser Asn Cys Ser Arg 
    530                 535                 540                 


Asp Cys Gln Ala Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr 
545                 550                 555                 560 


Cys Cys Phe Glu Cys Val Glu Cys Pro Asp Gly Glu Tyr Ser Gly Glu 
                565                 570                 575     


Thr Asp Ala Ser Ala Cys Asp Lys Cys Pro Asp Asp Phe Trp Ser Asn 
            580                 585                 590         


Glu Asn His Thr Ser Cys Ile Ala Lys Glu Ile Glu Phe Leu Ala Trp 
        595                 600                 605             


Thr Glu Pro Phe 
    610         


<210>  13
<211>  612
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human CasR ECD

<400>  13

Met Ala Phe Tyr Ser Cys Cys Trp Val Leu Leu Ala Leu Thr Trp His 
1               5                   10                  15      


Thr Ser Ala Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile 
            20                  25                  30          


Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp 
        35                  40                  45              


Gln Asp Leu Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Ser Ser Pro Ala Leu Leu Pro Asn Leu Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Thr Gly Ser Gly Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp 
        195                 200                 205             


Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln His Val Val 
                245                 250                 255     


Glu Val Ile Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser 
            260                 265                 270         


Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Gly Lys Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu 
    290                 295                 300                 


Ile Ala Met Pro Gln Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe 
305                 310                 315                 320 


Ala Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Lys Lys 
                325                 330                 335     


Val His Pro Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu 
        355                 360                 365             


Pro Val Asp Thr Phe Leu Arg Gly His Glu Glu Ser Gly Asp Arg Phe 
    370                 375                 380                 


Ser Asn Ser Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn 
385                 390                 395                 400 


Ile Ser Ser Val Glu Thr Pro Tyr Ile Asp Tyr Thr His Leu Arg Ile 
                405                 410                 415     


Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln 
            420                 425                 430         


Asp Ile Tyr Thr Cys Leu Pro Gly Arg Gly Leu Phe Thr Asn Gly Ser 
        435                 440                 445             


Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu 
    450                 455                 460                 


Arg His Leu Asn Phe Thr Asn Asn Met Gly Glu Gln Val Thr Phe Asp 
465                 470                 475                 480 


Glu Cys Gly Asp Leu Val Gly Asn Tyr Ser Ile Ile Asn Trp His Leu 
                485                 490                 495     


Ser Pro Glu Asp Gly Ser Ile Val Phe Lys Glu Val Gly Tyr Tyr Asn 
            500                 505                 510         


Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Glu Lys Ile 
        515                 520                 525             


Leu Trp Ser Gly Phe Ser Arg Glu Val Pro Phe Ser Asn Cys Ser Arg 
    530                 535                 540                 


Asp Cys Leu Ala Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr 
545                 550                 555                 560 


Cys Cys Phe Glu Cys Val Glu Cys Pro Asp Gly Glu Tyr Ser Asp Glu 
                565                 570                 575     


Thr Asp Ala Ser Ala Cys Asn Lys Cys Pro Asp Asp Phe Trp Ser Asn 
            580                 585                 590         


Glu Asn His Thr Ser Cys Ile Ala Lys Glu Ile Glu Phe Leu Ser Trp 
        595                 600                 605             


Thr Glu Pro Phe 
    610         


<210>  14
<211>  622
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gibbon CaSR ECD

<400>  14

Met Ala Phe Tyr Ser Cys Cys Trp Val Leu Leu Ala Leu Thr Trp His 
1               5                   10                  15      


Thr Ser Ala Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile 
            20                  25                  30          


Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp 
        35                  40                  45              


Gln Asp Leu Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Ser Ser Pro Ala Leu Leu Pro Asn Leu Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Thr Gly Ser Gly Val Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp 
        195                 200                 205             


Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln His Val Val 
                245                 250                 255     


Glu Val Ile Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser 
            260                 265                 270         


Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Gly Lys Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu 
    290                 295                 300                 


Ile Ala Met Pro Gln Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe 
305                 310                 315                 320 


Ala Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Lys Lys 
                325                 330                 335     


Val His Pro Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu 
        355                 360                 365             


Pro Val Asp Thr Phe Leu Arg Gly His Glu Glu Ser Gly Gly Arg Phe 
    370                 375                 380                 


Ser Asn Ser Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn 
385                 390                 395                 400 


Ile Ser Ser Val Glu Thr Pro Tyr Ile Asp Tyr Thr His Leu Arg Ile 
                405                 410                 415     


Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln 
            420                 425                 430         


Asp Ile Tyr Thr Cys Leu Pro Gly Arg Gly Leu Phe Thr Asn Gly Ser 
        435                 440                 445             


Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu 
    450                 455                 460                 


Arg His Leu Asn Phe Thr Asn Asn Met Gly Glu Gln Val Thr Phe Asp 
465                 470                 475                 480 


Glu Cys Gly Asp Leu Val Gly Asn Tyr Ser Ile Ile Asn Trp His Leu 
                485                 490                 495     


Ser Ala Glu Asp Gly Ser Ile Val Phe Lys Glu Val Gly Tyr Tyr Asn 
            500                 505                 510         


Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Glu Lys Ile 
        515                 520                 525             


Leu Trp Ser Gly Phe Ser Arg Glu Pro Leu Thr Phe Val Leu Ser Val 
    530                 535                 540                 


Pro Gln Val Pro Phe Ser Asn Cys Ser Arg Asp Cys Leu Ala Gly Thr 
545                 550                 555                 560 


Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr Cys Cys Phe Glu Cys Val 
                565                 570                 575     


Glu Cys Pro Asp Gly Glu Tyr Ser Asp Glu Thr Asp Ala Ser Ala Cys 
            580                 585                 590         


Asn Lys Cys Pro Asp Asp Phe Trp Ser Asn Glu Asn His Thr Ser Cys 
        595                 600                 605             


Ile Ala Lys Glu Ile Glu Phe Leu Ser Trp Thr Glu Pro Phe 
    610                 615                 620         


<210>  15
<211>  612
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dog CaSR ECD

<400>  15

Met Ala Phe His Ser Cys Ser Leu Ile Leu Leu Ala Ile Thr Trp Cys 
1               5                   10                  15      


Thr Ser Ala Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile 
            20                  25                  30          


Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp 
        35                  40                  45              


Gln Asp Leu Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Ser Ser Pro Ala Leu Leu Pro Asn Met Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Thr Gly Ser Gly Ile Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp 
        195                 200                 205             


Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln Gln Val Val 
                245                 250                 255     


Glu Val Ile Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser 
            260                 265                 270         


Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Gly Arg Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu 
    290                 295                 300                 


Ile Ala Met Pro Glu Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe 
305                 310                 315                 320 


Ala Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Gln Lys 
                325                 330                 335     


Val His Pro Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu 
        355                 360                 365             


Ser Met Asp Thr Phe Leu Arg Gly His Glu Glu Gly Gly Gly Arg Ile 
    370                 375                 380                 


Ser Asn Ser Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn 
385                 390                 395                 400 


Ile Ser Ser Val Glu Thr Pro Tyr Met Asp Tyr Thr His Leu Arg Ile 
                405                 410                 415     


Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln 
            420                 425                 430         


Asp Ile Tyr Thr Cys Leu Pro Gly Arg Gly Leu Phe Thr Asn Gly Ser 
        435                 440                 445             


Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu 
    450                 455                 460                 


Arg His Leu Asn Phe Thr Asn Asn Met Gly Glu Gln Val Thr Phe Asp 
465                 470                 475                 480 


Glu Cys Gly Asp Leu Met Gly Asn Tyr Ser Ile Ile Asn Trp His Leu 
                485                 490                 495     


Ser Pro Glu Asp Gly Ser Ile Val Phe Lys Glu Val Gly Tyr Tyr Asn 
            500                 505                 510         


Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Glu Lys Ile 
        515                 520                 525             


Leu Trp Ser Gly Phe Ser Arg Glu Met Pro Phe Ser Asn Cys Ser Arg 
    530                 535                 540                 


Asp Cys Leu Ala Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr 
545                 550                 555                 560 


Cys Cys Phe Glu Cys Val Glu Cys Pro Asp Gly Glu Tyr Ser Asp Glu 
                565                 570                 575     


Thr Asp Ala Ser Ala Cys Asp Lys Cys Pro Asp Asp Phe Trp Ser Asn 
            580                 585                 590         


Glu Asn His Thr Ser Cys Ile Ala Lys Glu Ile Glu Phe Leu Ser Trp 
        595                 600                 605             


Thr Glu Pro Phe 
    610         


<210>  16
<211>  553
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bovine CaSR ECD

<400>  16

Met Ala Leu Tyr Ser Cys Cys Trp Ile Leu Leu Ala Phe Ser Thr Trp 
1               5                   10                  15      


Cys Thr Ser Ala Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp 
            20                  25                  30          


Ile Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ala Val Lys 
        35                  40                  45              


Asp Gln Asp Leu Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr 
    50                  55                  60                  


Asn Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu 
65                  70                  75                  80  


Glu Ile Asn Ser Ser Pro Ala Leu Leu Pro Asn Met Thr Leu Gly Tyr 
                85                  90                  95      


Arg Ile Phe Asp Thr Cys Asn Thr Gln Val Ser Tyr Ala Ser Ser Ser 
            100                 105                 110         


Arg Leu Leu Ser Asn Lys Asn Gln Phe Lys Ser Phe Leu Arg Thr Ile 
        115                 120                 125             


Pro Asn Asp Glu His Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr 
    130                 135                 140                 


Phe Arg Trp Asn Trp Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly 
145                 150                 155                 160 


Arg Pro Gly Ile Glu Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile 
                165                 170                 175     


Cys Ile Asp Phe Ser Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Lys 
            180                 185                 190         


Ile Gln Gln Val Val Glu Val Ile Gln Asn Ser Thr Ala Lys Val Ile 
        195                 200                 205             


Val Val Phe Ser Ser Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile 
    210                 215                 220                 


Val Arg Arg Asn Ile Thr Gly Arg Ile Trp Leu Ala Ser Glu Ala Trp 
225                 230                 235                 240 


Ala Ser Ser Ser Leu Ile Ala Met Pro Glu Tyr Phe His Val Val Gly 
                245                 250                 255     


Gly Thr Ile Gly Phe Gly Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg 
            260                 265                 270         


Glu Phe Leu Gln Lys Val His Pro Arg Lys Ser Val His Asn Gly Phe 
        275                 280                 285             


Ala Lys Glu Phe Trp Glu Glu Thr Phe Asn Cys His Leu Gln Glu Gly 
    290                 295                 300                 


Ala Lys Gly Pro Leu Pro Val Asp Thr Phe Leu Arg Gly His Glu Glu 
305                 310                 315                 320 


Gly Gly Ala Arg Leu Ser Asn Ser Pro Thr Ala Phe Arg Pro Leu Cys 
                325                 330                 335     


Thr Gly Glu Glu Asn Ile Ser Ser Val Glu Thr Pro Tyr Met Asp Tyr 
            340                 345                 350         


Thr His Leu Arg Ile Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile 
        355                 360                 365             


Ala His Ala Leu Gln Asp Ile Tyr Thr Cys Ile Pro Gly Arg Gly Leu 
    370                 375                 380                 


Phe Thr Asn Gly Ser Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln 
385                 390                 395                 400 


Val Leu Lys His Leu Arg His Leu Asn Phe Thr Ser Asn Met Gly Glu 
                405                 410                 415     


Gln Val Thr Phe Asp Glu Cys Gly Asp Leu Ala Gly Asn Tyr Ser Ile 
            420                 425                 430         


Ile Asn Trp His Leu Ser Pro Glu Asp Gly Ser Ile Val Phe Lys Glu 
        435                 440                 445             


Val Gly Tyr Tyr Asn Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile 
    450                 455                 460                 


Asn Asp Glu Lys Ile Leu Trp Ser Gly Phe Ser Arg Glu Val Pro Phe 
465                 470                 475                 480 


Ser Asn Cys Ser Arg Asp Cys Leu Ala Gly Thr Arg Lys Gly Ile Ile 
                485                 490                 495     


Glu Gly Glu Pro Thr Cys Cys Phe Glu Cys Val Glu Cys Pro Asp Gly 
            500                 505                 510         


Glu Tyr Ser Asp Glu Thr Asp Ala Ser Ala Cys Asp Lys Cys Pro Asp 
        515                 520                 525             


Asp Phe Trp Ser Asn Glu Asn His Thr Ser Cys Ile Ala Lys Glu Ile 
    530                 535                 540                 


Glu Phe Leu Ser Trp Thr Glu Pro Phe 
545                 550             


<210>  17
<211>  612
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Alpaca CaSR ECD

<400>  17

Met Ala Ser Tyr Ser Cys Cys Trp Ile Leu Leu Ala Phe Ala Trp Cys 
1               5                   10                  15      


Ala Ser Ala Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile 
            20                  25                  30          


Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp 
        35                  40                  45              


Gln Asp Leu Lys Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Ser Ser Pro Ala Leu Leu Pro Asn Met Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Thr Gly Ser Gly Ile Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp 
        195                 200                 205             


Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln Gln Val Val 
                245                 250                 255     


Glu Val Ile Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser 
            260                 265                 270         


Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Gly Arg Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu 
    290                 295                 300                 


Ile Ala Met Pro Glu Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe 
305                 310                 315                 320 


Ala Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Gln Lys 
                325                 330                 335     


Val His Pro Arg Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu 
        355                 360                 365             


Pro Val Asp Asn Phe Leu Arg Gly His Glu Glu Gly Gly Gly Arg Thr 
    370                 375                 380                 


Ser Asn Ser Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn 
385                 390                 395                 400 


Ile Ser Ser Val Glu Thr Pro Tyr Met Asp Tyr Thr His Leu Arg Ile 
                405                 410                 415     


Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln 
            420                 425                 430         


Asp Ile Tyr Thr Cys Ile Pro Gly Arg Gly Leu Phe Thr Asn Gly Ser 
        435                 440                 445             


Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu 
    450                 455                 460                 


Arg His Leu Asn Phe Thr Asn Asn Met Gly Glu Gln Val Thr Phe Asp 
465                 470                 475                 480 


Glu Cys Gly Asp Leu Ala Gly Asn Tyr Ser Ile Ile Asn Trp His Leu 
                485                 490                 495     


Ser Pro Glu Asp Gly Ser Ile Val Phe Lys Glu Val Gly Tyr Tyr Asn 
            500                 505                 510         


Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Glu Lys Ile 
        515                 520                 525             


Leu Trp Ser Gly Phe Ser Arg Glu Val Pro Phe Ser Asn Cys Ser Arg 
    530                 535                 540                 


Asp Cys Leu Ala Gly Thr Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr 
545                 550                 555                 560 


Cys Cys Phe Glu Cys Val Glu Cys Pro Asp Gly Glu Tyr Ser Asp Glu 
                565                 570                 575     


Thr Asp Ala Ser Ala Cys Asp Lys Cys Pro Asp Asp Phe Trp Ser Asn 
            580                 585                 590         


Glu Asn His Thr Ser Cys Ile Ala Lys Glu Ile Glu Phe Leu Ser Trp 
        595                 600                 605             


Thr Glu Pro Phe 
    610         


<210>  18
<211>  622
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pig CaSR ECD

<400>  18

Met Ala Phe Ser Ser Cys Cys Trp Ile Leu Leu Ala Leu Thr Trp Cys 
1               5                   10                  15      


Thr Ser Ala Tyr Gly Pro Asp Gln Arg Ala Gln Lys Lys Gly Asp Ile 
            20                  25                  30          


Ile Leu Gly Gly Leu Phe Pro Ile His Phe Gly Val Ala Ala Lys Asp 
        35                  40                  45              


Gln Asn Leu Glu Ser Arg Pro Glu Ser Val Glu Cys Ile Arg Tyr Asn 
    50                  55                  60                  


Phe Arg Gly Phe Arg Trp Leu Gln Ala Met Ile Phe Ala Ile Glu Glu 
65                  70                  75                  80  


Ile Asn Ser Ser Pro Ala Leu Leu Pro Asn Met Thr Leu Gly Tyr Arg 
                85                  90                  95      


Ile Phe Asp Thr Cys Asn Thr Val Ser Lys Ala Leu Glu Ala Thr Leu 
            100                 105                 110         


Ser Phe Val Ala Gln Asn Lys Ile Asp Ser Leu Asn Leu Asp Glu Phe 
        115                 120                 125             


Cys Asn Cys Ser Glu His Ile Pro Ser Thr Ile Ala Val Val Gly Ala 
    130                 135                 140                 


Thr Gly Ser Gly Ile Ser Thr Ala Val Ala Asn Leu Leu Gly Leu Phe 
145                 150                 155                 160 


Tyr Ile Pro Gln Val Ser Tyr Ala Ser Ser Ser Arg Leu Leu Ser Asn 
                165                 170                 175     


Lys Asn Gln Phe Lys Ser Phe Leu Arg Thr Ile Pro Asn Asp Glu His 
            180                 185                 190         


Gln Ala Thr Ala Met Ala Asp Ile Ile Glu Tyr Phe Arg Trp Asn Trp 
        195                 200                 205             


Val Gly Thr Ile Ala Ala Asp Asp Asp Tyr Gly Arg Pro Gly Ile Glu 
    210                 215                 220                 


Lys Phe Arg Glu Glu Ala Glu Glu Arg Asp Ile Cys Ile Asp Phe Ser 
225                 230                 235                 240 


Glu Leu Ile Ser Gln Tyr Ser Asp Glu Glu Glu Ile Gln Gln Val Val 
                245                 250                 255     


Glu Val Ile Gln Asn Ser Thr Ala Lys Val Ile Val Val Phe Ser Ser 
            260                 265                 270         


Gly Pro Asp Leu Glu Pro Leu Ile Lys Glu Ile Val Arg Arg Asn Ile 
        275                 280                 285             


Thr Gly Lys Ile Trp Leu Ala Ser Glu Ala Trp Ala Ser Ser Ser Leu 
    290                 295                 300                 


Ile Ala Met Pro Glu Tyr Phe His Val Val Gly Gly Thr Ile Gly Phe 
305                 310                 315                 320 


Ala Leu Lys Ala Gly Gln Ile Pro Gly Phe Arg Glu Phe Leu Gln Lys 
                325                 330                 335     


Val His Pro Ser Lys Ser Val His Asn Gly Phe Ala Lys Glu Phe Trp 
            340                 345                 350         


Glu Glu Thr Phe Asn Cys His Leu Gln Glu Gly Ala Lys Gly Pro Leu 
        355                 360                 365             


Thr Thr Asp Thr Phe Leu Arg Gly His Glu Glu Gly Gly Gly Arg Ile 
    370                 375                 380                 


Ser Asn Ser Ser Thr Ala Phe Arg Pro Leu Cys Thr Gly Asp Glu Asn 
385                 390                 395                 400 


Ile Ser Ser Val Glu Thr Pro Tyr Met Asp Tyr Thr His Leu Arg Ile 
                405                 410                 415     


Ser Tyr Asn Val Tyr Leu Ala Val Tyr Ser Ile Ala His Ala Leu Gln 
            420                 425                 430         


Asp Ile Tyr Thr Cys Ile Pro Gly Arg Gly Leu Phe Thr Asn Gly Ser 
        435                 440                 445             


Cys Ala Asp Ile Lys Lys Val Glu Ala Trp Gln Val Leu Lys His Leu 
    450                 455                 460                 


Arg His Leu Asn Phe Thr Ser Asn Met Gly Glu Gln Val Thr Phe Asp 
465                 470                 475                 480 


Glu Tyr Gly Asp Leu Ala Gly Asn Tyr Ser Ile Ile Asn Trp His Leu 
                485                 490                 495     


Ser Pro Glu Asp Gly Ser Ile Val Phe Lys Glu Val Gly Tyr Tyr Asn 
            500                 505                 510         


Val Tyr Ala Lys Lys Gly Glu Arg Leu Phe Ile Asn Glu Glu Lys Ile 
        515                 520                 525             


Leu Trp Ser Gly Phe Ser Arg Glu Pro Leu Thr Val Val Leu Pro Ile 
    530                 535                 540                 


Leu Gln Val Pro Phe Ser Asn Cys Ser Arg Asp Cys Leu Ala Gly Thr 
545                 550                 555                 560 


Arg Lys Gly Ile Ile Glu Gly Glu Pro Thr Cys Cys Phe Glu Cys Val 
                565                 570                 575     


Glu Cys Pro Asp Gly Glu Tyr Ser Asp Glu Thr Asp Ala Ser Ala Cys 
            580                 585                 590         


Asp Lys Cys Pro Asp Asp Phe Trp Ser Asn Glu Asn His Thr Ser Cys 
        595                 600                 605             


Ile Ala Lys Glu Ile Glu Phe Leu Ser Trp Thr Glu Pro Phe 
    610                 615                 620         


