                               SEQUENCE LISTING

<110> STCUBE & CO., INC.
      BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
 
<120> METHODS FOR SELECTING ANTIBODIES THAT SPECIFICALLY BIND 
      GLYCOSYLATED IMMUNE CHECKPOINT PROTEINS

<130> 24258.105006PCT

<140>
<141>

<150> 62/361,298
<151> 2016-07-12

<150> 62/314,940
<151> 2016-03-29

<160> 19    

<170> PatentIn version 3.5

<210> 1
<211> 290
<212> PRT
<213> Homo sapiens

<400> 1
Met Arg Ile Phe Ala Val Phe Ile Phe Met Thr Tyr Trp His Leu Leu 
1               5                   10                  15      


Asn Ala Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
        35                  40                  45              


Asp Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile 
    50                  55                  60                  


Ile Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser 
65                  70                  75                  80  


Tyr Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Arg Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val 
        115                 120                 125             


Lys Val Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val 
    130                 135                 140                 


Asp Pro Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr 
145                 150                 155                 160 


Pro Lys Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser 
                165                 170                 175     


Gly Lys Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn 
            180                 185                 190         


Val Thr Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr 
        195                 200                 205             


Cys Thr Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu 
    210                 215                 220                 


Val Ile Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg Thr His 
225                 230                 235                 240 


Leu Val Ile Leu Gly Ala Ile Leu Leu Cys Leu Gly Val Ala Leu Thr 
                245                 250                 255     


Phe Ile Phe Arg Leu Arg Lys Gly Arg Met Met Asp Val Lys Lys Cys 
            260                 265                 270         


Gly Ile Gln Asp Thr Asn Ser Lys Lys Gln Ser Asp Thr His Leu Glu 
        275                 280                 285             


Glu Thr 
    290 


<210> 2
<211> 288
<212> PRT
<213> Homo sapiens

<400> 2
Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             


Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 


Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 


Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     


Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         


Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             


<210> 3
<211> 289
<212> PRT
<213> Homo sapiens

<400> 3
Met Lys Thr Leu Pro Ala Met Leu Gly Thr Gly Lys Leu Phe Trp Val 
1               5                   10                  15      


Phe Phe Leu Ile Pro Tyr Leu Asp Ile Trp Asn Ile His Gly Lys Glu 
            20                  25                  30          


Ser Cys Asp Val Gln Leu Tyr Ile Lys Arg Gln Ser Glu His Ser Ile 
        35                  40                  45              


Leu Ala Gly Asp Pro Phe Glu Leu Glu Cys Pro Val Lys Tyr Cys Ala 
    50                  55                  60                  


Asn Arg Pro His Val Thr Trp Cys Lys Leu Asn Gly Thr Thr Cys Val 
65                  70                  75                  80  


Lys Tyr Leu Glu Arg Gln Thr Ser Trp Lys Glu Glu Lys Asn Ile Ser 
                85                  90                  95      


Phe Phe Ile Leu His Phe Glu Pro Val Leu Pro Asn Asp Asn Gly Ser 
            100                 105                 110         


Tyr Arg Cys Ser Ala Asn Phe Gln Ser Asn Leu Ile Glu Ser His Ser 
        115                 120                 125             


Thr Thr Leu Tyr Val Thr Asp Val Lys Ser Ala Ser Glu Arg Pro Ser 
    130                 135                 140                 


Lys Asp Glu Met Ala Ser Arg Pro Trp Leu Leu Tyr Arg Leu Leu Pro 
145                 150                 155                 160 


Leu Gly Gly Leu Pro Leu Leu Ile Thr Thr Cys Phe Cys Leu Phe Cys 
                165                 170                 175     


Cys Leu Arg Arg His Gln Gly Lys Gln Asn Glu Leu Ser Asp Thr Ala 
            180                 185                 190         


Gly Arg Glu Ile Asn Leu Val Asp Ala His Leu Lys Ser Glu Gln Thr 
        195                 200                 205             


Glu Ala Ser Thr Arg Gln Asn Ser Gln Val Leu Leu Ser Glu Thr Gly 
    210                 215                 220                 


Ile Tyr Asp Asn Asp Pro Asp Leu Cys Phe Arg Met Gln Glu Gly Ser 
225                 230                 235                 240 


Glu Val Tyr Ser Asn Pro Cys Leu Glu Glu Asn Lys Pro Gly Ile Val 
                245                 250                 255     


Tyr Ala Ser Leu Asn His Ser Val Ile Gly Pro Asn Ser Arg Leu Ala 
            260                 265                 270         


Arg Asn Val Lys Glu Ala Pro Thr Glu Tyr Ala Ser Ile Cys Val Arg 
        275                 280                 285             


Ser 
    


<210> 4
<211> 187
<212> PRT
<213> Homo sapiens

<400> 4
Ser Glu Val Glu Tyr Arg Ala Glu Val Gly Gln Asn Ala Tyr Leu Pro 
1               5                   10                  15      


Cys Phe Tyr Thr Pro Ala Ala Pro Gly Asn Leu Val Pro Val Cys Trp 
            20                  25                  30          


Gly Lys Gly Ala Cys Pro Val Phe Glu Cys Gly Asn Val Val Leu Arg 
        35                  40                  45              


Thr Asp Glu Arg Asp Val Asn Tyr Trp Thr Ser Arg Tyr Trp Leu Asn 
    50                  55                  60                  


Gly Asp Phe Arg Lys Gly Asp Val Ser Leu Thr Ile Glu Asn Val Thr 
65                  70                  75                  80  


Leu Ala Asp Ser Gly Ile Tyr Cys Cys Arg Ile Gln Ile Pro Gly Ile 
                85                  90                  95      


Met Asn Asp Glu Lys Phe Asn Leu Lys Leu Val Ile Lys Pro Ala Lys 
            100                 105                 110         


Val Thr Pro Ala Pro Thr Leu Gln Arg Asp Phe Thr Ala Ala Phe Pro 
        115                 120                 125             


Arg Met Leu Thr Thr Arg Gly His Gly Pro Ala Glu Thr Gln Thr Leu 
    130                 135                 140                 


Gly Ser Leu Pro Asp Ile Asn Leu Thr Gln Ile Ser Thr Leu Ala Asn 
145                 150                 155                 160 


Glu Leu Arg Asp Ser Arg Leu Ala Asn Asp Leu Arg Asp Ser Gly Ala 
                165                 170                 175     


Thr Ile Arg Val Asp His His His His His His 
            180                 185         


<210> 5
<211> 412
<212> PRT
<213> Homo sapiens

<400> 5
Leu Gln Pro Gly Ala Glu Val Pro Val Val Trp Ala Gln Glu Gly Ala 
1               5                   10                  15      


Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile Pro Leu Gln Asp Leu Ser 
            20                  25                  30          


Leu Leu Arg Arg Ala Gly Val Thr Trp Gln His Gln Pro Asp Ser Gly 
        35                  40                  45              


Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His Pro 
    50                  55                  60                  


Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Arg Arg Tyr Thr Val Leu 
65                  70                  75                  80  


Ser Val Gly Pro Gly Gly Leu Arg Ser Gly Arg Leu Pro Leu Gln Pro 
                85                  90                  95      


Arg Val Gln Leu Asp Glu Arg Gly Arg Gln Arg Gly Asp Phe Ser Leu 
            100                 105                 110         


Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala Gly Glu Tyr Arg Ala Ala 
        115                 120                 125             


Val His Leu Arg Asp Arg Ala Leu Ser Cys Arg Leu Arg Leu Arg Leu 
    130                 135                 140                 


Gly Gln Ala Ser Met Thr Ala Ser Pro Pro Gly Ser Leu Arg Ala Ser 
145                 150                 155                 160 


Asp Trp Val Ile Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg Pro Ala 
                165                 170                 175     


Ser Val His Trp Phe Arg Asn Arg Gly Gln Gly Arg Val Pro Val Arg 
            180                 185                 190         


Glu Ser Pro His His His Leu Ala Glu Ser Phe Leu Phe Leu Pro Gln 
        195                 200                 205             


Val Ser Pro Met Asp Ser Gly Pro Trp Gly Cys Ile Leu Thr Tyr Arg 
    210                 215                 220                 


Asp Gly Phe Asn Val Ser Ile Met Tyr Asn Leu Thr Val Leu Gly Leu 
225                 230                 235                 240 


Glu Pro Pro Thr Pro Leu Thr Val Tyr Ala Gly Ala Gly Ser Arg Val 
                245                 250                 255     


Gly Leu Pro Cys Arg Leu Pro Ala Gly Val Gly Thr Arg Ser Phe Leu 
            260                 265                 270         


Thr Ala Lys Trp Thr Pro Pro Gly Gly Gly Pro Asp Leu Leu Val Thr 
        275                 280                 285             


Gly Asp Asn Gly Asp Phe Thr Leu Arg Leu Glu Asp Val Ser Gln Ala 
    290                 295                 300                 


Gln Ala Gly Thr Tyr Thr Cys His Ile His Leu Gln Glu Gln Gln Leu 
305                 310                 315                 320 


Asn Ala Thr Val Thr Leu Ala Ile Ile Thr Val Thr Pro Lys Ser Phe 
                325                 330                 335     


Gly Ser Pro Gly Ser Leu Gly Lys Leu Leu Cys Glu Val Thr Pro Val 
            340                 345                 350         


Ser Gly Gln Glu Arg Phe Val Trp Ser Ser Leu Asp Thr Pro Ser Gln 
        355                 360                 365             


Arg Ser Phe Ser Gly Pro Trp Leu Glu Ala Gln Glu Ala Gln Leu Leu 
    370                 375                 380                 


Ser Gln Pro Trp Gln Cys Gln Leu Tyr Gln Gly Glu Arg Leu Leu Gly 
385                 390                 395                 400 


Ala Ala Val Tyr Phe Thr Glu Leu Ser Ser Pro Gly 
                405                 410         


<210> 6
<211> 526
<212> PRT
<213> Homo sapiens

<400> 6
Met Ala Val Phe Pro Ser Ser Gly Leu Pro Arg Cys Leu Leu Thr Leu 
1               5                   10                  15      


Ile Leu Leu Gln Leu Pro Lys Leu Asp Ser Ala Pro Phe Asp Val Ile 
            20                  25                  30          


Gly Pro Pro Glu Pro Ile Leu Ala Val Val Gly Glu Asp Ala Lys Leu 
        35                  40                  45              


Pro Cys Arg Leu Ser Pro Asn Ala Ser Ala Glu His Leu Glu Leu Arg 
    50                  55                  60                  


Trp Phe Arg Lys Lys Val Ser Pro Ala Val Leu Val His Arg Asp Gly 
65                  70                  75                  80  


Arg Glu Gln Glu Ala Glu Gln Met Pro Glu Tyr Arg Gly Arg Ala Thr 
                85                  90                  95      


Leu Val Gln Asp Gly Ile Ala Lys Gly Arg Val Ala Leu Arg Ile Arg 
            100                 105                 110         


Gly Val Arg Val Ser Asp Asp Gly Glu Tyr Thr Cys Phe Phe Arg Glu 
        115                 120                 125             


Asp Gly Ser Tyr Glu Glu Ala Leu Val His Leu Lys Val Ala Ala Leu 
    130                 135                 140                 


Gly Ser Asp Pro His Ile Ser Met Gln Val Gln Glu Asn Gly Glu Ile 
145                 150                 155                 160 


Cys Leu Glu Cys Thr Ser Val Gly Trp Tyr Pro Glu Pro Gln Val Gln 
                165                 170                 175     


Trp Arg Thr Ser Lys Gly Glu Lys Phe Pro Ser Thr Ser Glu Ser Arg 
            180                 185                 190         


Asn Pro Asp Glu Glu Gly Leu Phe Thr Val Ala Ala Ser Val Ile Ile 
        195                 200                 205             


Arg Asp Thr Ser Ala Lys Asn Val Ser Cys Tyr Ile Gln Asn Leu Leu 
    210                 215                 220                 


Leu Gly Gln Glu Lys Lys Val Glu Ile Ser Ile Pro Ala Ser Ser Leu 
225                 230                 235                 240 


Pro Arg Leu Thr Pro Trp Ile Val Ala Val Ala Val Ile Leu Met Val 
                245                 250                 255     


Leu Gly Leu Leu Thr Ile Gly Ser Ile Phe Phe Thr Trp Arg Leu Tyr 
            260                 265                 270         


Asn Glu Arg Pro Arg Glu Arg Arg Asn Glu Phe Ser Ser Lys Glu Arg 
        275                 280                 285             


Leu Leu Glu Glu Leu Lys Trp Lys Lys Ala Thr Leu His Ala Val Asp 
    290                 295                 300                 


Val Thr Leu Asp Pro Asp Thr Ala His Pro His Leu Phe Leu Tyr Glu 
305                 310                 315                 320 


Asp Ser Lys Ser Val Arg Leu Glu Asp Ser Arg Gln Lys Leu Pro Glu 
                325                 330                 335     


Lys Thr Glu Arg Phe Asp Ser Trp Pro Cys Val Leu Gly Arg Glu Thr 
            340                 345                 350         


Phe Thr Ser Gly Arg His Tyr Trp Glu Val Glu Val Gly Asp Arg Thr 
        355                 360                 365             


Asp Trp Ala Ile Gly Val Cys Arg Glu Asn Val Met Lys Lys Gly Phe 
    370                 375                 380                 


Asp Pro Met Thr Pro Glu Asn Gly Phe Trp Ala Val Glu Leu Tyr Gly 
385                 390                 395                 400 


Asn Gly Tyr Trp Ala Leu Thr Pro Leu Arg Thr Pro Leu Pro Leu Ala 
                405                 410                 415     


Gly Pro Pro Arg Arg Val Gly Ile Phe Leu Asp Tyr Glu Ser Gly Asp 
            420                 425                 430         


Ile Ser Phe Tyr Asn Met Asn Asp Gly Ser Asp Ile Tyr Thr Phe Ser 
        435                 440                 445             


Asn Val Thr Phe Ser Gly Pro Leu Arg Pro Phe Phe Cys Leu Trp Ser 
    450                 455                 460                 


Ser Gly Lys Lys Pro Leu Thr Ile Cys Pro Ile Ala Asp Gly Pro Glu 
465                 470                 475                 480 


Arg Val Thr Val Ile Ala Asn Ala Gln Asp Leu Ser Lys Glu Ile Pro 
                485                 490                 495     


Leu Ser Pro Met Gly Glu Asp Ser Ala Pro Arg Asp Ala Asp Thr Leu 
            500                 505                 510         


His Ser Lys Leu Ile Pro Thr Gln Pro Ser Gln Gly Ala Pro 
        515                 520                 525     


<210> 7
<211> 468
<212> PRT
<213> Homo sapiens

<400> 7
Met Gly His Leu Ser Ala Pro Leu His Arg Val Arg Val Pro Trp Gln 
1               5                   10                  15      


Gly Leu Leu Leu Thr Ala Ser Leu Leu Thr Phe Trp Asn Pro Pro Thr 
            20                  25                  30          


Thr Ala Gln Leu Thr Thr Glu Ser Met Pro Phe Asn Val Ala Glu Gly 
        35                  40                  45              


Lys Glu Val Leu Leu Leu Val His Asn Leu Pro Gln Gln Leu Phe Gly 
    50                  55                  60                  


Tyr Ser Trp Tyr Lys Gly Glu Arg Val Asp Gly Asn Arg Gln Ile Val 
65                  70                  75                  80  


Gly Tyr Ala Ile Gly Thr Gln Gln Ala Thr Pro Gly Pro Ala Asn Ser 
                85                  90                  95      


Gly Arg Glu Thr Ile Tyr Pro Asn Ala Ser Leu Leu Ile Gln Asn Val 
            100                 105                 110         


Thr Gln Asn Asp Thr Gly Phe Tyr Thr Leu Gln Val Ile Lys Ser Asp 
        115                 120                 125             


Leu Val Asn Glu Glu Ala Thr Gly Gln Phe His Val Tyr Pro Glu Leu 
    130                 135                 140                 


Pro Lys Pro Ser Ile Ser Ser Asn Asn Ser Asn Pro Val Glu Asp Lys 
145                 150                 155                 160 


Asp Ala Val Ala Phe Thr Cys Glu Pro Glu Thr Gln Asp Thr Thr Tyr 
                165                 170                 175     


Leu Trp Trp Ile Asn Asn Gln Ser Leu Pro Val Ser Pro Arg Leu Gln 
            180                 185                 190         


Leu Ser Asn Gly Asn Arg Thr Leu Thr Leu Leu Ser Val Thr Arg Asn 
        195                 200                 205             


Asp Thr Gly Pro Tyr Glu Cys Glu Ile Gln Asn Pro Val Ser Ala Asn 
    210                 215                 220                 


Arg Ser Asp Pro Val Thr Leu Asn Val Thr Tyr Gly Pro Asp Thr Pro 
225                 230                 235                 240 


Thr Ile Ser Pro Ser Asp Thr Tyr Tyr Arg Pro Gly Ala Asn Leu Ser 
                245                 250                 255     


Leu Ser Cys Tyr Ala Ala Ser Asn Pro Pro Ala Gln Tyr Ser Trp Leu 
            260                 265                 270         


Ile Asn Gly Thr Phe Gln Gln Ser Thr Gln Glu Leu Phe Ile Pro Asn 
        275                 280                 285             


Ile Thr Val Asn Asn Ser Gly Ser Tyr Thr Cys His Ala Asn Asn Ser 
    290                 295                 300                 


Val Thr Gly Cys Asn Arg Thr Thr Val Lys Thr Ile Ile Val Thr Glu 
305                 310                 315                 320 


Leu Ser Pro Val Val Ala Lys Pro Gln Ile Lys Ala Ser Lys Thr Thr 
                325                 330                 335     


Val Thr Gly Asp Lys Asp Ser Val Asn Leu Thr Cys Ser Thr Asn Asp 
            340                 345                 350         


Thr Gly Ile Ser Ile Arg Trp Phe Phe Lys Asn Gln Ser Leu Pro Ser 
        355                 360                 365             


Ser Glu Arg Met Lys Leu Ser Gln Gly Asn Thr Thr Leu Ser Ile Asn 
    370                 375                 380                 


Pro Val Lys Arg Glu Asp Ala Gly Thr Tyr Trp Cys Glu Val Phe Asn 
385                 390                 395                 400 


Pro Ile Ser Lys Asn Gln Ser Asp Pro Ile Met Leu Asn Val Asn Tyr 
                405                 410                 415     


Asn Ala Leu Pro Gln Glu Asn Gly Leu Ser Pro Gly Ala Ile Ala Gly 
            420                 425                 430         


Ile Val Ile Gly Val Val Ala Leu Val Ala Leu Ile Ala Val Ala Leu 
        435                 440                 445             


Ala Cys Phe Leu His Phe Gly Lys Thr Gly Arg Thr Thr Pro Met Thr 
    450                 455                 460                 


His Leu Thr Arg 
465             


<210> 8
<211> 862
<212> PRT
<213> Homo sapiens

<400> 8
Met Arg Met Cys Thr Pro Ile Arg Gly Leu Leu Met Ala Leu Ala Val 
1               5                   10                  15      


Met Phe Gly Thr Ala Met Ala Phe Ala Pro Ile Pro Arg Ile Thr Trp 
            20                  25                  30          


Glu His Arg Glu Val His Leu Val Gln Phe His Glu Pro Asp Ile Tyr 
        35                  40                  45              


Asn Tyr Ser Ala Leu Leu Leu Ser Glu Asp Lys Asp Thr Leu Tyr Ile 
    50                  55                  60                  


Gly Ala Arg Glu Ala Val Phe Ala Val Asn Ala Leu Asn Ile Ser Glu 
65                  70                  75                  80  


Lys Gln His Glu Val Tyr Trp Lys Val Ser Glu Asp Lys Lys Ala Lys 
                85                  90                  95      


Cys Ala Glu Lys Gly Lys Ser Lys Gln Thr Glu Cys Leu Asn Tyr Ile 
            100                 105                 110         


Arg Val Leu Gln Pro Leu Ser Ala Thr Ser Leu Tyr Val Cys Gly Thr 
        115                 120                 125             


Asn Ala Phe Gln Pro Ala Cys Asp His Leu Asn Leu Thr Ser Phe Lys 
    130                 135                 140                 


Phe Leu Gly Lys Asn Glu Asp Gly Lys Gly Arg Cys Pro Phe Asp Pro 
145                 150                 155                 160 


Ala His Ser Tyr Thr Ser Val Met Val Asp Gly Glu Leu Tyr Ser Gly 
                165                 170                 175     


Thr Ser Tyr Asn Phe Leu Gly Ser Glu Pro Ile Ile Ser Arg Asn Ser 
            180                 185                 190         


Ser His Ser Pro Leu Arg Thr Glu Arg Ala Ala Asn Tyr Thr Ser Ser 
        195                 200                 205             


Leu Asn Leu Pro Asp Lys Thr Leu Gln Phe Val Lys Asp His Pro Leu 
    210                 215                 220                 


Met Ser His Thr Lys Trp Val Arg Tyr Asn Gly Pro Val Pro Lys Pro 
225                 230                 235                 240 


Arg Pro Gly Ala Cys Ile Asp Ser Glu Ala Cys Ala Tyr Asn Leu Ser 
                245                 250                 255     


Thr Ala Glu Glu Val Phe Ser His Gly Lys Tyr Met Gln Ser Thr Thr 
            260                 265                 270         


Val Glu Gln Ser Pro Gly Leu Lys Val Pro Val Phe Tyr Ala Leu Phe 
        275                 280                 285             


Thr Pro Gln Leu Asn Asn Val Gly Leu Ser Ala Val Lys Ala Arg Leu 
    290                 295                 300                 


Ile Cys Ser Arg Pro Asp Ser Gly Leu Val Phe Asn Val Leu Arg Asp 
305                 310                 315                 320 


Val Phe Val Leu Arg Pro Arg Ile Ala Arg Val Cys Lys Gly Asp Gln 
                325                 330                 335     


Gly Gly Leu Arg Thr Leu Gln Lys Lys Trp Thr Ser Phe Leu Gly Glu 
            340                 345                 350         


Asp Asp Arg Val Tyr Phe Phe Phe Thr Glu Val Ser Val Glu Tyr Glu 
        355                 360                 365             


Phe Val Phe Arg Val Leu Ile Tyr Ala Ile Pro Trp Leu Asn Glu Pro 
    370                 375                 380                 


Ser Phe Val Phe Ala Asp Val Ile Arg Lys Ser Pro Asp Ser Pro Asp 
385                 390                 395                 400 


Asp Asp Ser Val Thr Pro Ile Asp Asn Arg Pro Arg Leu Ile Lys Lys 
                405                 410                 415     


Asp Val Asn Tyr Thr Gln Ile Val Val Asp Arg Thr Gln Ala Leu Asp 
            420                 425                 430         


Gly Thr Val Tyr Asp Val Met Phe Val Ser Thr Asp Arg Gly Ala Leu 
        435                 440                 445             


His Lys Ala Ile Ser Leu Glu His Ala Val His Ile Ile Glu Glu Thr 
    450                 455                 460                 


Gln Leu Phe Gln Asp Phe Glu Pro Val Gln Thr Leu Leu Leu Ser Ser 
465                 470                 475                 480 


Lys Lys Gly Asn Arg Phe Val Tyr Ala Gly Ser Asn Ser Gly Val Val 
                485                 490                 495     


Gln Ala Pro Leu Ala Phe Cys Gly Lys His Gly Thr Cys Glu Asp Cys 
            500                 505                 510         


Val Leu Ala Arg Asp Pro Tyr Cys Ala Trp Ser Pro Pro Thr Ala Thr 
        515                 520                 525             


Cys Val Ala Leu His Gln Thr Glu Ser Pro Ser Arg Gly Leu Ile Gln 
    530                 535                 540                 


Glu Met Ser Gly Asp Ala Ser Val Cys Pro Asp Lys Ser Lys Gly Ser 
545                 550                 555                 560 


Tyr Arg Gln His Phe Phe Lys His Gly Gly Thr Ala Glu Leu Lys Cys 
                565                 570                 575     


Ser Gln Lys Ser Asn Leu Ala Arg Val Phe Trp Lys Phe Gln Asn Asp 
            580                 585                 590         


Val Leu Lys Ala Glu Ser Pro Lys Tyr Gly Leu Met Gly Arg Lys Asn 
        595                 600                 605             


Leu Leu Ile Phe Asn Leu Ser Glu Gly Asp Ser Gly Val Tyr Gln Cys 
    610                 615                 620                 


Leu Ser Glu Glu Arg Val Lys Asn Lys Thr Val Phe Gln Val Val Ala 
625                 630                 635                 640 


Lys His Val Leu Glu Val Lys Val Val Pro Lys Pro Val Val Ala Pro 
                645                 650                 655     


Thr Leu Ser Val Val Gln Thr Glu Gly Ser Arg Ile Ala Thr Lys Val 
            660                 665                 670         


Leu Val Ala Ser Thr Gln Gly Ser Ser Pro Pro Thr Pro Ala Val Gln 
        675                 680                 685             


Ala Thr Ser Ser Gly Ala Ile Thr Leu Pro Pro Lys Pro Ala Pro Thr 
    690                 695                 700                 


Gly Thr Ser Cys Glu Pro Lys Ile Val Ile Asn Thr Val Pro Gln Leu 
705                 710                 715                 720 


His Ser Glu Lys Thr Met Tyr Leu Lys Ser Ser Asp Asn Arg Leu Leu 
                725                 730                 735     


Met Ser Leu Phe Leu Phe Phe Phe Val Leu Phe Leu Cys Leu Phe Phe 
            740                 745                 750         


Tyr Asn Cys Tyr Lys Gly Tyr Leu Pro Arg Gln Cys Leu Lys Phe Arg 
        755                 760                 765             


Ser Ala Leu Leu Ile Gly Lys Lys Lys Pro Lys Ser Asp Phe Cys Asp 
    770                 775                 780                 


Arg Glu Gln Ser Leu Lys Glu Thr Leu Val Glu Pro Gly Ser Phe Ser 
785                 790                 795                 800 


Gln Gln Asn Gly Glu His Pro Lys Pro Ala Leu Asp Thr Gly Tyr Glu 
                805                 810                 815     


Thr Glu Gln Asp Thr Ile Thr Ser Lys Val Pro Thr Asp Arg Glu Asp 
            820                 825                 830         


Ser Gln Arg Ile Asp Asp Leu Ser Ala Arg Asp Lys Pro Phe Asp Val 
        835                 840                 845             


Lys Cys Glu Leu Lys Phe Ala Asp Ser Asp Ala Asp Gly Asp 
    850                 855                 860         


<210> 9
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 9
tcaattgtca tattgctac                                                    19


<210> 10
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
ttgactccat ctttcttca                                                    19


<210> 11
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
gtggtagagt atggtagcca aatgacaatt gaatgcaaa                              39


<210> 12
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
tttgcattca attgtcattt ggctaccata ctctaccac                              39


<210> 13
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
gagaggagaa gcttttccag gtgaccagca cactgag                                37


<210> 14
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
ctcagtgtgc tggtcacctg gaaaagcttc tcctctc                                37


<210> 15
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
gaccagcaca ctgagaatcc agacaacaac taatgagat                              39


<210> 16
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
atctcattag ttgttgtctg gattctcagt gtgctggtc                              39


<210> 17
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
gagagaggag aagcttttcc aagtgaccag cacactgaga                             40


<210> 18
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
tctcagtgtg ctggtcactt ggaaaagctt ctcctctctc                             40


<210> 19
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 19
His His His His His His 
1               5       


