Sequence Listing St. 25 format


<110> CSIR
 
<120> Nucleotide sequence encoding 9-lipoxygenase and recombinant constructs comprising the same 

<150> 201611011374

<151> 31/03/2016
 
<160> 11

<170> PatentIn version 3.5

<210> 1
<211> 2526
<212> DNA
<213> Artificial sequence 

<220>
<223> Recombinant DNA 

<400> 1

atggggacag tggtgttgat gaagaagaat tttttggact tcaatgacct cagtgcatcg     60

gttattgatc gtgttgatga actggttggt aaaagagtct ctttgcagct cgttagtgct    120 

tttaactctg accctactgc atcaaatggg ctgcaaggaa aggttggaga gccagcacat    180

ttggaagagt ggattactac aatcactcct ttggtagcag aggaatcgac attcaaggtc    240

acatttgatt gggatgaaca gattggagtt ccaggagcat tcataataaa gaacaatcat    300

cacagtggat tttacttgaa atctctcaca cttgaggatg ttcctaatca gggtcggatt    360

cactttgtat gcaactcctg ggtttatcct gcaaaacgct ataagaaaga tcgcgttttc    420

ttcaccaaca agacatacct tccaggtgaa atgccggcac cattacaata ttatagagag    480

caagaactcc taaacttgag aggagatgga actggagagc ttcaagaatg ggacagagtc    540

tatgactatg cgtactataa tgatttgggt gatccggaca atggcaaacc acgaccagtt    600 

cttggagggt ctactgagta tccttatcct cgtaggggaa gaacaggcag accaccagca    660

aaaacagatc ctgagactga gagcaggctg ccacttctga cgagcttaaa catctatgtt    720

ccaagagatg agcgatttgg tcacataaaa atgtcagatt ttcttggtta tgcactgaaa    780 

tccatatctc aattcattga gccagcgttg gaatctgtat ttgacagcac cccaaatgaa    840
 
tttgacaact ttgctcaaat atacaaactc tacgatgaag ggattcagct tcctaatgac    900 

cattttcttg atgatattag aaataatatc cccttagaat tgctcaagga gatttttcca    960

accaatgagg ataatctctt tgaattccca acaccacagg tgatccaagg ggataggtct   1020 

gcatggagaa ccgatgaaga gtttgcaaga gaaatgctgg ctggaccgaa ccctgtgatc   1080 

attcgccgag ttgaggaatt ccctccaaga agcaagctcg accctgaact atatggtgat   1140

caagatagta agataaccaa acagcacata gagagctact tagatgggct gactgtagag   1200

caggcaattg agaagaacga gctattcata ttggatcacc atgattcact gatgacatac   1260

ttgagaagga taaacactac ttccacaaag acttatgcat ccaggacaat ccttttctta   1320

aaagaggatg gaactttgaa accactggca attgaattga gcaggccaca tcctgatgga   1380 

gatcaatatg gtgccatcag caacgtttac acgccatcag aagatgaagt ggaaggttcc   1440

atatggcagc tggctaaagc ttatgtggct gtaaatgact ctggtgttca tcagctcatc   1500 

agccactggt tgaagactca tgcagcaatt gagccatttg tgatagcaac aaatcggcaa   1560

ctgagtgtgc ttcacccaat ttataagctt ctgcaacctc atttccgtga cacaatgaat   1620

ataaatgcgt ttgctcgtca gatcgtcatt aatgcgggtg gaattctgga aactacggtt   1680

ttccctgcaa agtatgccat ggaaatgtca tctgcaatct acaaagactg gacttttcca   1740

gatcaggcac ttcctgaaga cctcaagaat agaggaatgg cagttgagga ccccaactct   1800

ccacatggtc ttcgcctact gatagcagac tacccatatg ctgttgatgg gcttgaaatc   1860

tggtttgcaa taaaaaactg ggtcaaagac tattgctact tctactacaa aagcgatgaa   1920

atgatgcaaa aggatagtgg actgcaatcc tggtggaagg aactacgcga ggagggtcat   1980 

ggtgacaaga aagatgagcc ctggtggcct aaaatgcaaa atcgtgaaga gctgatagag   2040

gcatgcacca taatcatatg gatagcttcc gctctccatg ctgctgtcaa ttttggacag   2100

tatccttatg caggtttcct ccctaaccgt ccaactatga gtcgaagatt catgcctgaa   2160

aaaggaactc ctgattatga tgagctagag tcgaatttcg acaaagtgtt cctgaaaacg   2220

atcactgctc agcggcagac tcttcttggc attgctctta tagagacttt gtcaaggcat   2280 

tcatcggatg agatgtacct gggacaaaga gacacacctg aatggacatc agataaaatt   2340 

cccttgcaag catttgagga cttcggaaag aaattgggag acattgaagt aagaatcata   2400

acaagaaatc atgacaatat gctcaagaac cgcgttggcc ctgtcaatat gccatacact   2460 

ttgctttatc ctaccagtga aggtggcctt actggcaaag gaattccaaa cagtgtttca   2520 

atttag                                                              2526                  



<210> 2

<211> 841

<212> PRT
<213> Artificial sequence 

<220>

<223> Protein sequence of 7- Dehydrocholestrol reductase (7-DHCR)

<400> 2

Met Gly Thr Val Val Leu Met Lys Lys Asn Phe Leu Asp Phe Asn Asp

Leu Ser Ala Ser Val Ile Asp Arg Val Asp Glu Leu Val Gly Lys Arg 

Val Ser Leu Gln Leu Val Ser Ala Phe Asn Ser Asp Pro Thr Ala Ser

Asn Gly Leu Gln Gly Lys Val Gly Glu Pro Ala His Leu Glu Glu Trp 

Ile Thr Thr Ile Thr Pro Leu Val Ala Glu Glu Ser Thr Phe Lys Val 

Thr Phe Asp Trp Asp Glu Gln Ile Gly Val Pro Gly Ala Phe Ile Ile 

Lys Asn Asn His His Ser Gly Phe Tyr Leu Lys Ser Leu Thr Leu Glu 

Asp Val Pro Asn Gln Gly Arg Ile His Phe Val Cys Asn Ser Trp Val 

Tyr Pro Ala Lys Arg Tyr Lys Lys Asp Arg Val Phe Phe Thr Asn Lys 

Thr Tyr Leu Pro Gly Glu Met Pro Ala Pro Leu Gln Tyr Tyr Arg Glu

Gln Glu Leu Leu Asn Leu Arg Gly Asp Gly Thr Gly Glu Leu Gln Glu 

Trp Asp Arg Val Tyr Asp Tyr Ala Tyr Tyr Asn Asp Leu Gly Asp Pro 

Asp Asn Gly Lys Pro Arg Pro Val Leu Gly Gly Ser Thr Glu Tyr Pro 

Tyr Pro Arg Arg Gly Arg Thr Gly Arg Pro Pro Ala Lys Thr Asp Pro 

Glu Thr Glu Ser Arg Leu Pro Leu Leu Thr Ser Leu Asn Ile Tyr Val

Pro Arg Asp Glu Arg Phe Gly His Ile Lys Met Ser Asp Phe Leu Gly 

Tyr Ala Leu Lys Ser Ile Ser Gln Phe Ile Glu Pro Ala Leu Glu Ser 

Val Phe Asp Ser Thr Pro Asn Glu Phe Asp Asn Phe Ala Gln Ile Tyr 

Lys Leu Tyr Asp Glu Gly Ile Gln Leu Pro Asn Asp His Phe Leu Asp 

Asp Ile Arg Asn Asn Ile Pro Leu Glu Leu Leu Lys Glu Ile Phe Pro 

Thr Asn Glu Asp Asn Leu Phe Glu Phe Pro Thr Pro Gln Val Ile Gln 

Gly Asp Arg Ser Ala Trp Arg Thr Asp Glu Glu Phe Ala Arg Glu Met 

Leu Ala Gly Pro Asn Pro Val Ile Ile Arg Arg Val Glu Glu Phe Pro 

Pro Arg Ser Lys Leu Asp Pro Glu Leu Tyr Gly Asp Gln Asp Ser Lys 

Ile Thr Lys Gln His Ile Glu Ser Tyr Leu Asp Gly Leu Thr Val Glu 

Gln Ala Ile Glu Lys Asn Glu Leu Phe Ile Leu Asp His His Asp Ser 

Leu Met Thr Tyr Leu Arg Arg Ile Asn Thr Thr Ser Thr Lys Thr Tyr 

Ala Ser Arg Thr Ile Leu Phe Leu Lys Glu Asp Gly Thr Leu Lys Pro 

Leu Ala Ile Glu Leu Ser Arg Pro His Pro Asp Gly Asp Gln Tyr Gly 

Ala Ile Ser Asn Val Tyr Thr Pro Ser Glu Asp Glu Val Glu Gly Ser 

Ile Trp Gln Leu Ala Lys Ala Tyr Val Ala Val Asn Asp Ser Gly Val 

His Gln Leu Ile Ser His Trp Leu Lys Thr His Ala Ala Ile Glu Pro 

Phe Val Ile Ala Thr Asn Arg Gln Leu Ser Val Leu His Pro Ile Tyr 

Lys Leu Leu Gln Pro His Phe Arg Asp Thr Met Asn Ile Asn Ala Phe
 
Ala Arg Gln Ile Val Ile Asn Ala Gly Gly Ile Leu Glu Thr Thr Val

Phe Pro Ala Lys Tyr Ala Met Glu Met Ser Ser Ala Ile Tyr Lys Asp 

Trp Thr Phe Pro Asp Gln Ala Leu Pro Glu Asp Leu Lys Asn Arg Gly 

Met Ala Val Glu Asp Pro Asn Ser Pro His Gly Leu Arg Leu Leu Ile 

Ala Asp Tyr Pro Tyr Ala Val Asp Gly Leu Glu Ile Trp Phe Ala Ile 

Lys Asn Trp Val Lys Asp Tyr Cys Tyr Phe Tyr Tyr Lys Ser Asp Glu 

Met Met Gln Lys Asp Ser Gly Leu Gln Ser Trp Trp Lys Glu Leu Arg 

Glu Glu Gly His Gly Asp Lys Lys Asp Glu Pro Trp Trp Pro Lys Met 

Gln Asn Arg Glu Glu Leu Ile Glu Ala Cys Thr Ile Ile Ile Trp Ile 

Ala Ser Ala Leu His Ala Ala Val Asn Phe Gly Gln Tyr Pro Tyr Ala 

Gly Phe Leu Pro Asn Arg Pro Thr Met Ser Arg Arg Phe Met Pro Glu 

Lys Gly Thr Pro Asp Tyr Asp Glu Leu Glu Ser Asn Phe Asp Lys Val 

Phe Leu Lys Thr Ile Thr Ala Gln Arg Gln Thr Leu Leu Gly Ile Ala 

Leu Ile Glu Thr Leu Ser Arg His Ser Ser Asp Glu Met Tyr Leu Gly 

Gln Arg Asp Thr Pro Glu Trp Thr Ser Asp Lys Ile Pro Leu Gln Ala 

Phe Glu Asp Phe Gly Lys Lys Leu Gly Asp Ile Glu Val Arg Ile Ile 

Thr Arg Asn His Asp Asn Met Leu Lys Asn Arg Val Gly Pro Val Asn 

Met Pro Tyr Thr Leu Leu Tyr Pro Thr Ser Glu Gly Gly Leu Thr Gly 

Lys Gly Ile Pro Asn Ser Val Ser Ile



<210> 3

<211> 4499

<212> DNA

<213> Mangifera indica
<220>

<223> DNA 

<400> 1

atggggacag tggtgttgat gaagaagaat tttttggact tcaatgacct cagtgcatcg        60

gttattgatc gtgttgatga actggttggt aaaagagtct ctttgcagct cgttagtgct       120

tttaactctg accctactgc atgtgagttc ttcttctttt ctctttactt gcaccttttt       180

cttttttaga tctctggttt cttctgctct ttttcttggg tttgatgctg ctgtttgtgg       240

ccacaaaaaa agaaagcctg cagcttcctc accatgcaat aactattcat gtgtgaaaag       300

ggaaaaatgt gcagaaactt gaagaacaaa cttcttttct atttgtcttt ggtttgaaaa       360

tattacaagt agtaagttag tttgatgtgg gcatttaaaa ctcacctttt cagttagttt       420

ttgggattga gagagactcg atattacttt tcgaggcctt aacacagtcc ttgtggttcg       480

aggtcgaact atagtaaaat cccaaaccag ggacggtcgt ttcttatgat atccaacgtt       540

ttctctatga atttatagca tatctctata tttgactttg gggaagtttt gtgttatctc       600

tatatatatg atgaggaata agagtttggc ctatagtttt tactgtcttc tgtcctcttt       660

tgggtaaaaa ggacagatga gtcatgataa aaatggctat gtaaattgaa caccgtaata       720

tgttcacaga gatatagtta tggcagttca taaatatgag ctttaacaag ttttttccat       780

taatgaattg tgttaccaaa agaagattcg gcagaagcag ggcagggtct gaactgtttc       840

actaattgct tgtttgtttt taggtgacaa ttgaaagttg gaggtctccc aagtctcaaa       900

cagaaaaaag ttatcattta atcggccttt ttctttgttt cttattttaa agtcgactag       960

aatattacca agtctttgtt tttttacttg taatctaact tgacaaatgg ggcatgacct      1020

atttgacatt ttcttatgtt ttcaaaacac aattcatcaa aactaacaaa tgttataatt      1080

taatataatt tcaaggttaa accatatatt aggtaaaact ccctatctta aaaacctaaa      1140

tgccaaatct gaacaggatg atgattaaaa tcctttttaa ttattaaatc atattgactt      1200

aaaattaaat tgattggata gtattaaatc ataattcaat tgttgagtta gattgatctg      1260

atcgttacaa tgatgtcatt cggatgctac ataattaaaa tttattttaa aactttgttg      1320

attgatcctt tgaccaagtc aacgttgatc caatttaaaa acatggggta aattatatta      1380

attttcctga taattaaacc tgggtcaata attttggcag ttcattgatt gttaagaagt      1440

tgtaatgaag tgatgtaatt catttcatca aagccttaat agttagagaa taaaaaatag      1500

gaacttgaag agggaatgaa gaatttttta ccttttcaat gaatatgcca ttaatttatt      1560

tttgaactat cacctaattt gaaaatgaaa ttaatgagtg tgtcaatgaa tatttgatgt      1620

gtgtgtgtgg atgcagcaaa tgggctgcaa ggaaaggttg gagagccagc acatttggaa      1680

gagtggatta ctacaatcac tcctttggta gcagaggaat cgacattcaa ggtcacattt      1740

gattgggatg aacagattgg agttccagga gcattcataa taaagaacaa tcatcacagt      1800

ggattttact tgaaatctct cacacttgag gatgttccta atcagggtcg gattcacttt      1860

gtatgcaact cctgggttta tcctgcaaaa cgctataaga aagatcgcgt tttcttcacc      1920

aacaaggcaa gtatagtttt gatacaatta atttcctaca agtgattatg agtcttgtac      1980

taagtttatg tcaactattt ttctgcagac ataccttcca ggtgaaatgc cggcaccatt      2040

acaatattat agagagcaag aactcctaaa cttgagagga gatggaactg gagagcttca      2100

agaatgggac agagtctatg actatgcgta ctataatgat ttgggtgatc cggacaatgg      2160

caaaccacga ccagttcttg gagggtctac tgagtatcct tatcctcgta ggggaagaac      2220

aggcagacca ccagcaaaaa caggtaccgt tccattggta tgtttggaga aatattaagt      2280

cttatcagtg caattggcat tgtcattgac agtttggtta ttgttgtaac agatcctgag      2340

actgagagca ggctgccact tctgacgagc ttaaacatct atgtcccaag agatgagcga      2400

tttggtcaca taaaaatgtc agattttctt ggttatgcac tgaaatccat atctcaattc      2460

attgagccag cgttggaatc tgtatttgac agcaccccaa atgaatttga caactttgct      2520

caaatataca aactctacga tgaagggatt cagcttccta atgaccattt tcttgatgat      2580

attagaaata atatcccctt agaattgctc aaggagattt ttccaaccaa tgaggataat      2640

ctctttgaat tcccaacacc acaggtgatc caaggtaatg tgagacattt ctagccgatc      2700

ctctatttgc catataacag atttaagttt gtgcttctat acatttttca ttttcctgtt      2760

tttaatgttt taataccttg gagcagggga taggtctgca tggagaaccg atgaagagtt      2820

tgcaagagaa atgctggctg gaccgaaccc tgtcatcatt cgccgagttg aggtaaagat      2880

tttgtaacaa gttttctttc tctcattttc ttcttcacca agaaagtaga tgccttgtga      2940

tattttctga taaaaattac taaaataatt gcagcaattc cctccaagaa gcaagctcga      3000

ccctgaacta tatggtgatc aagatagtaa gataaccaaa cagcacatag agagctactt      3060

agatgggctg actgtagagc aggtacccca acagtcttgt gatccttttg tgattgtaat      3120

ttcttaatat gagtctttaa ccaaacaaat taacaagtga tttcgatttg gtataggcaa      3180

ttgagaagaa caagctattc atattggatc accatgattc actgatgaca tacttgagaa      3240

ggataaacac tacttccaca aagacttatg catccaggac aatccttttc ttaaaagagg      3300

atggaacttt gaaaccactg gcaattgaat tgagcaggcc acatcctgat ggagatcaat      3360

atggtgccat cagcaacgtt tacacgccat cagaagatga agtggaaggt tccatatggc      3420

agctggctaa agcttatgtg gctgtaaatg actctggtgt tcatcagctc atcagccact      3480

ggttgaagac tcatgcagca attgagccat ttgtgatagc aacaaatcgg caactgagtg      3540

tgcttcaccc aatttataag cttctgcaac ctcatttccg tgacacaatg aatataaatg      3600

cgtttgctcg tcagatcgtc attaatgcgg gtggaattct ggaaactacg gttttccctg      3660

caaagtatgc catggaaatg tcatctgcaa tctacaaaga ctggactttt ccagatcagg      3720

cacttcctga agacctcaag aatagaggaa tggcagttga ggaccccaac tctccacatg      3780

gtcttcgcct actgatagca gactacccat atgctgttga tgggcttgaa atctggtttg      3840

caataaaaaa ctgggtcaaa gactattgct acttctacta caaaagcgat gaaatgatgc      3900

aaaaggatag tggactgcaa tcctggtgga aggaactacg cgaggagggt catggtgaca      3960

agaaagatga gccctggtgg cctaaaatgc aaaatcgtga agagctgata gaggcatgca      4020

ccataatcat atggatagct tccgctctcc atgctgctgt caattttgga cagtatcctt      4080

atgcaggttt cctccctaac cgtccaacta tgagtcgaag attcatgcct gaaaaaggaa      4140

ctcctgatta tgatgagcta gagtcgaatt tcgacaaagt gttcctgaaa acgatcactg      4200

ctcagcggca gactcttctt ggcattgctc ttatagagac tttgtcaagg cattcatcgg      4260

atgagatgta cctgggacaa agagacacac ctgaatggac atcagataaa atccccttgc      4320

aagcattcga ggacttcgga aagaaattgg gagacattga agtaagaatc ataacaagaa      4380

atcatgacaa tatgctcaag aaccgcgttg gccctgtcaa tgtgccatac actttgcttt      4440

atcctaccag tgaaggtggc cttactggca aaggaattcc aaacagtgtt tcaatttag       4499



<210> 4

<211> 22
<212> DNA
<213> Artificial sequence 
<220>
<223> LF1_primer 
<400> 4

gggatccgga caatggcaaa cc


<210> 5

<211> 19
<212> DNA
<213> Artificial sequence 
<220>
<223> LR3_primer 
<400> 19

cctccaagaa ctggtcgtg


<210> 6

<211> 24
<212> DNA
<213> Artificial sequence 
<220>
<223> LOX_TF1_primer 
<400> 24

atggggacag tggtgttgat gaag 


<210> 7

<211> 27
<212> DNA
<213> Artificial sequence 
<220>
<223> LOX_TR1_primer 
<400> 27

ctaaattgaa acactgtttg gaattcc


<210> 8

<211> 28
<212> DNA
<213> Artificial sequence 
<220>
<223> LOX_ pET101D F1
<400> 28

caccatgggg acagtggtgt tgatgaag


<210> 9

<211> 28
<212> DNA
<213> Artificial sequence 
<223> LOX_ pET101D R1
<400> 28

aattgaaaca ctgtttggaa ttcctttg


<210> 10

<211> 36

<212> DNA

<213> Artificial sequence

<223> LOXpBI121_F1

<400> 36

aaaaaaggat ccatggggac agtggtgttg atgaag


<210> 11

<211> 39

<212> DNA

<213> Artificial sequence

<223> LOXpBI121_R1

<400> 39
aaaaaaggat ccctaaattg aaacactgtt tggaattcc
