                         SEQUENCE LISTING

<110>  FUNDACIN CIENCIA PARA LA VIDA
 
<120>  ATTENUATION OF NEURODEGENERATION ASSOCIATED WITH PARKINSON'S 
       DISEASE BY INHIBITION OF THE DOPAMINE D3 RECEPTOR IN CD4+ T CELLS

<130>  73978-PCT

<140>  PCT/IB2017/051868
<141>  2017-07-19

<150>  PCT/IB2017/051868
<151>  2017-03-31

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  small hairpin RNA (shRNA) against dopamine receptor D3 (DRD3)

<400>  1
tgccctctcc tctttggttt caacacaac                                         29


<210>  2
<211>  1373
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Region inserted into retroviral vector pBullet: 5'-CCATGGCC 
       (additional 5' NcoI restriction site sequence) / Green 
       fluorescent protein (GFP) / U6 promoter / shRNA against DRD3 / H1
       promoter / GTCGAC-3' (additional 3' SalI restriction site 
       

<400>  2
ccatggccgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc       60

tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca      120

cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc      180

ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca      240

tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca      300

tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca      360

ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg      420

ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga      480

agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc      540

tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca      600

accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca      660

tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca      720

agtaaagcgc tttatttagc gggcagtgca ccaactacgc cctgctgaag ctggccggcg      780

acgtggagag caaccccggc cccggatcca tcgccaaggt cgggcaggaa gagggcctat      840

ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa      900

ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat      960

ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg     1020

taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga caaaaatagt     1080

cttctctgcc ctctcctctt tggtttcaac acaacgaaga cagctttttt tggtgtttcg     1140

tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt ttatggtgat     1200

ttcccagaac acatagcgac atgcaaatat tgcagggcgc cactcccctg tccctcacag     1260

ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca ctgggcccgc     1320

gattccttgg agcgggttga tgacgtcagc gttctcaatt gagcgctgtc gac            1373


<210>  3
<211>  5296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Retroviral vector pBullet sequence: 5'-GTCGAC (additional 5' 
       sequence corresponding to SalI restriction site) / Retroviral 
       vector pBullet / CCATGG-3' (additional 3' sequence corresponding 
       to NcoI restriction site)

<400>  3
gtcgacgaga ccggattagt ccaatttgtt aaagacaggt atcagtggtc caggctctag       60

ttttgactaa caatatcacc agctgaagcc tatagtagag ccatagataa aataaaagat      120

tttattagtc tccagaaaaa gggggaatga aagacccacc tgtaggtttg gcaagctagc      180

ttaataacgc cattttgcaa ggcatggaaa aatcataact gagaatagag aagttcagat      240

caggtcagga acagatggaa cagctgaata tggccaaaca ggatatctgt ggtaagcagt      300

tcctgccccg gctcagggcc aagaacagat gaacagctga atatgggcca aacaggatac      360

tgtggtaagc agttcctgcc ccggctcagg ccaagaacag atggtcccca gatgcggcca      420

gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat      480

gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt      540

ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga      600

ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact      660

tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg      720

gtctttcaca ccatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtattaca      780

ttaaatggcc atagtctgct cgatcaggag ctttttgcaa aagcctaggc ctccaaaagc      840

ctcttcacta cttctggaat agccagaggc cgaggcggcc tcggcctctg caaaataaaa      900

tagaaattag tcagccatgc aggggcggag aatgggcgga actgggcgga ttaggggcgg      960

gatgggcgga gttaggggcg gactatggtt gctgactaat tgctgcataa tgaatcggcc     1020

aacgcgcggg gagaggcgtt tgcgtattgg gcgctcttcc gcttcccgct cactgactcg     1080

ctgcgctcgg tcgttggctg cggcgagcgg tatcagctca ctcaaggcgg taatacggtt     1140

atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaggccag caaaaggcca     1200

ggaaccgtaa aaggccgcgt tgctggcgtt tttccatagg tccgcccccc tgacgagcat     1260

cacaaaaatg acgctcaagt cagaggtggc gaaacccgca ggactataaa gataccaagg     1320

cgtttcccct ggaagctccc tcgtgcgctc tcctgtccga ccctgccgct taccggatac     1380

ctgtcgcctt tctcccttcg ggaagcgtgg cgcttctcat agctcacgct gtaggtatct     1440

cagtcggtgt aggtcgttcg ctccaagctg ggtgtgtgca cgaacccccc gttcagcccg     1500

acgctgcgcc ttatccggta actatcgtct gagtccaacc cggtaagaca cgacttatcc     1560

cactggcagc agccactggt aacaggatag cagagcgagg tatgtaggcg gtgctacgag     1620

ttcttgaagt ggtggcctaa ctacggtaca ctagaaggac agtatttggt atctggctct     1680

gctgaagcca gttaccttcg gaaaagagtt ggtagctctt gatccggcaa acaaccaccg     1740

ctggtagcgg tggttttttt gttgcaagca gcagattacg cgcagaaaaa aaggatctca     1800

agaagatcct ttgatctttt cacggggtct gacgctcagt ggaacgaaaa tcacgttaag     1860

ggattttggt catgagattt caaaaaggat cttcacctag atccttttaa ttaaaaatga     1920

agttttaaat caatctaagt atatatgagt aaacttggtc tgacagtacc aatgcttaat     1980

cagtgaggca cctattcagc gatctgtcta tttcgttcat ccatgttgcc tgactccccg     2040

tcgtgtagat aacacgatac gggagggctt accatctggc ccagtgctgc aatgataccg     2100

cgagacccac gtcaccggct ccagatttat cagcaataaa cagccagccg gaagggccga     2160

gcgcagaagg gtcctgcaac tttatccgcc tccatccatc tattaattgt tgccgggaag     2220

ctagagtagt agttcgccag ttaatagttt gcgcaagttg ttgccattgc tacaggcatc     2280

gtggttcacg ctcgtcgttt ggtatggctt cattagctcc ggttcccaac gatcaaggcg     2340

agtacatgat cccccatgtt gtgcaaaaaa gcgttagctc cttcggtcct ccgatcgttg     2400

tagaagtaag ttggccgcag tgttatcact atggttatgg cagcactgca taattctcta     2460

ctgtcatgcc atccgtaaga tgcttttcgt gactggtgag tactcaacca agtcatttga     2520

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg     2580

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc     2640

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga     2700

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat     2760

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt     2820

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt     2880

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac     2940

gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc     3000

tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccgaga     3060

cggtcacagc ttgtctgtaa gcggagccgg gagcagacaa gcccgtcagg gcgctcagcg     3120

ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga     3180

gtgcaccatt cgacgctctc cctatgcgac tcctgcatag gaagcagccc agagtaggtt     3240

gaggccgttg agcaccgccg ccgcaaggaa tggtctggct tatcgaaatt aatacgactc     3300

actataggga gaccggaatt cccatcgata tcgatgggct agagtccgtt acataactta     3360

cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga     3420

cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt     3480

tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta     3540

ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg     3600

actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg catggtgatg     3660

cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt     3720

ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca     3780

aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag     3840

gtctatataa gcagagctcg ttagtgaacc ggcgccagtc ctccgattga ctgagtcgcc     3900

cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt     3960

tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg     4020

ggctcgtccg ggatcgggag acccctgccc aggaccaccg acccaccacc gggaggtaag     4080

ctggccagca acttattctg tgtctgtccg attgtctagt gtctatgact gattttatgc     4140

gcctgcgtcg gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg     4200

acgagttcgg aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt     4260

tttgtggccc gacctgagtc taaaatcccg atcgtttagg actctttggt gcacccccct     4320

tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt     4380

ctgaattttt gctttcggtt tgggaccgaa gccgcgcgtc ttgtctgctg cagcatcgtt     4440

ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggctag     4500

cctgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct     4560

cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg     4620

ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag     4680

gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggc ccctacatcg     4740

tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta     4800

agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga     4860

ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc     4920

catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga     4980

caagagttac taacagcccc tctctccaac tcacttacag gctctctact tagtccagca     5040

cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc     5100

tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacaa gaacctagaa     5160

cctcgctgga aaggacctac acagtcctgc tgaccacccc accgccctca aagtagacgg     5220

catcgcagct tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc     5280

ctctagactg ccatgg                                                     5296


