                         SEQUENCE LISTING

<110>  ZuvaSyntha Limited
 
<120>  Modified Enzyme

<130>  JWJ02205WO

<150>  1605354.8
<151>  2016-03-30

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  211
<212>  PRT
<213>  Consensus Sequence

<400>  1

Met Ala Lys Leu Ile Asp His Thr Leu Leu Lys Pro Asp Ala Thr Asp 
1               5                   10                  15      


Glu Asp Ile Ile Lys Leu Cys His Glu Ala Lys Glu Tyr Asn Phe Ala 
            20                  25                  30          


Ser Val Cys Val Asn Pro Arg Phe Val Pro Leu Ala Ala Glu Thr Leu 
        35                  40                  45              


Glu Gly Asp Val Lys Val Cys Thr Val Ile Gly Phe Pro Leu Gly Ala 
    50                  55                  60                  


Asp Thr Thr Ala Val Lys Ala Phe Glu Thr Lys Ala Ala Ile Ala Asn 
65                  70                  75                  80  


Gly Ala Asp Glu Ile Asp Met Val Ile Asn Ile Gly Ala Leu Lys Ala 
                85                  90                  95      


Gly Asn Glu Asp Val Val Glu Glu Asp Ile Lys Ala Val Val Glu Ala 
            100                 105                 110         


Cys Gly Val Leu Leu Lys Val Ile Ile Glu Thr Cys Leu Leu Thr Asp 
        115                 120                 125             


Glu Glu Ile Val Lys Ala Cys Glu Ile Ser Ile Lys Ala Gly Ala Asp 
    130                 135                 140                 


Phe Val Lys Thr Ser Thr Gly Phe Ser Thr Gly Gly Ala Thr Val Glu 
145                 150                 155                 160 


Asp Val Arg Leu Met Arg Glu Thr Val Gly Pro Asp Val Gly Val Lys 
                165                 170                 175     


Ala Ser Gly Gly Val Arg Thr Ala Glu Asp Ala Leu Ala Met Ile Glu 
            180                 185                 190         


Ala Gly Ala Thr Arg Ile Gly Ala Ser Ala Gly Val Ala Ile Val Lys 
        195                 200                 205             


Ser Gly Tyr 
    210     


<210>  2
<211>  259
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Thr Asp Leu Lys Ala Ser Ser Leu Arg Ala Leu Lys Leu Met Asp 
1               5                   10                  15      


Leu Thr Thr Leu Asn Asp Asp Asp Thr Asp Glu Lys Val Ile Ala Leu 
            20                  25                  30          


Cys His Gln Ala Lys Thr Pro Val Gly Asn Thr Ala Ala Ile Cys Ile 
        35                  40                  45              


Tyr Pro Arg Phe Ile Pro Ile Ala Arg Lys Thr Leu Lys Glu Gln Gly 
    50                  55                  60                  


Thr Pro Glu Ile Arg Thr Ala Thr Val Thr Asn Phe Pro His Gly Asn 
65                  70                  75                  80  


Asp Asp Ile Glu Ile Ala Leu Ala Glu Thr Arg Ala Ala Ile Ala Tyr 
                85                  90                  95      


Gly Ala Asp Glu Val Asp Val Val Phe Pro Tyr Arg Ala Leu Met Ala 
            100                 105                 110         


Gly Asn Glu Gln Val Gly Phe Asp Leu Val Lys Ala Cys Lys Glu Ala 
        115                 120                 125             


Cys Ala Ala Ala Asn Val Leu Leu Lys Val Ile Ile Glu Thr Gly Glu 
    130                 135                 140                 


Leu Lys Asp Glu Ala Leu Ile Arg Lys Ala Ser Glu Ile Ser Ile Lys 
145                 150                 155                 160 


Ala Gly Ala Asp Phe Ile Lys Thr Ser Thr Gly Lys Val Ala Val Asn 
                165                 170                 175     


Ala Thr Pro Glu Ser Ala Arg Ile Met Met Glu Val Ile Arg Asp Met 
            180                 185                 190         


Gly Val Glu Lys Thr Val Gly Phe Lys Pro Ala Gly Gly Val Arg Thr 
        195                 200                 205             


Ala Glu Asp Ala Gln Lys Tyr Leu Ala Ile Ala Asp Glu Leu Phe Gly 
    210                 215                 220                 


Ala Asp Trp Ala Asp Ala Arg His Tyr Arg Phe Gly Ala Ser Ser Leu 
225                 230                 235                 240 


Leu Ala Ser Leu Leu Lys Ala Leu Gly His Gly Asp Gly Lys Ser Ala 
                245                 250                 255     


Ser Ser Tyr 
            


<210>  3
<211>  275
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Ala His Asn Arg Gly Thr Glu Leu Asp Leu Ser Trp Ile Ser 
1               5                   10                  15      


Lys Ile Gln Val Asn His Pro Ala Val Leu Arg Arg Ala Glu Gln Ile 
            20                  25                  30          


Gln Ala Arg Arg Thr Val Lys Lys Glu Trp Gln Ala Ala Trp Leu Leu 
        35                  40                  45              


Lys Ala Val Thr Phe Ile Asp Leu Thr Thr Leu Ser Gly Asp Asp Thr 
    50                  55                  60                  


Ser Ser Asn Ile Gln Arg Leu Cys Tyr Lys Ala Lys Tyr Pro Ile Arg 
65                  70                  75                  80  


Glu Asp Leu Leu Lys Ala Leu Asn Met His Asp Lys Gly Ile Thr Thr 
                85                  90                  95      


Ala Ala Val Cys Val Tyr Pro Ala Arg Val Cys Asp Ala Val Lys Ala 
            100                 105                 110         


Leu Lys Ala Ala Gly Cys Asn Ile Pro Val Ala Ser Val Ala Ala Gly 
        115                 120                 125             


Phe Pro Ala Gly Gln Thr His Leu Lys Thr Arg Leu Glu Glu Ile Arg 
    130                 135                 140                 


Leu Ala Val Glu Asp Gly Ala Thr Glu Ile Asp Val Val Ile Asn Arg 
145                 150                 155                 160 


Ser Leu Val Leu Thr Gly Gln Trp Glu Gly Ser Asp Phe Ile Lys Thr 
                165                 170                 175     


Ser Thr Gly Lys Glu Thr Val Asn Ala Thr Phe Pro Val Ala Ile Val 
            180                 185                 190         


Met Leu Arg Ala Ile Arg Asp Phe Phe Trp Lys Thr Gly Asn Lys Ile 
        195                 200                 205             


Gly Phe Lys Pro Ala Gly Gly Ile Arg Ser Ala Lys Asp Ser Leu Ala 
    210                 215                 220                 


Trp Leu Ser Leu Val Lys Glu Glu Leu Gly Asp Glu Trp Leu Lys Pro 
225                 230                 235                 240 


Glu Leu Phe Arg Ile Gly Ala Ser Thr Leu Leu Ser Asp Ile Glu Arg 
                245                 250                 255     


Gln Ile Tyr His His Val Thr Gly Arg Tyr Ala Ala Tyr His Asp Leu 
            260                 265                 270         


Pro Met Ser 
        275 


<210>  4
<211>  263
<212>  PRT
<213>  Plasmodium falciparum

<400>  4

Met Ala Asn Tyr Ala Glu Thr Phe Ser Ala Trp Thr Ser Ile Cys Leu 
1               5                   10                  15      


Thr Asp His Thr Leu Leu Gly Glu Asn Asp Thr Glu Asp Asp Ile Arg 
            20                  25                  30          


Glu Leu Cys Glu Glu Ser Val Lys Thr Cys Pro Phe Ala Ala Ala Val 
        35                  40                  45              


Cys Val Lys Pro Glu Phe Val Lys Phe Ile Asn Asp Thr Ile Lys Lys 
    50                  55                  60                  


Glu Ile Cys Pro Phe Lys Pro Lys Val Ala Cys Val Ile Asn Phe Pro 
65                  70                  75                  80  


His Gly Thr Asp Val Val Glu Lys Val Leu Glu Asp Thr Lys Lys Val 
                85                  90                  95      


Leu Glu Asp Gly Val Asp Glu Ile Asp Leu Val Ile Asn Tyr Lys Lys 
            100                 105                 110         


Val Leu Gln Asn Val Glu Glu Gly Leu Lys Glu Ala Thr Glu Leu Thr 
        115                 120                 125             


Lys Lys Val Lys Asn Leu Met Lys Glu Lys Val Leu Lys Val Ile Ile 
    130                 135                 140                 


Glu Thr Gly Glu Leu Lys Asn Asp Glu Leu Ile Ile Lys Thr Thr Leu 
145                 150                 155                 160 


Ala Val Leu Glu Gly Asn Ala Asp Phe Val Lys Thr Ser Ser Gly Lys 
                165                 170                 175     


Val Pro Val Asn Ala Thr Pro Gly Ala Val Lys Ala Ile Ile Glu Ala 
            180                 185                 190         


Met Lys Gln Tyr Val Glu Lys Asn Pro Gln Lys Lys Asp Lys Ile Gly 
        195                 200                 205             


Leu Lys Val Ala Gly Gly Val Gly Asp Leu Asn Ser Ala Ser Tyr Tyr 
    210                 215                 220                 


Ile Leu Leu Ala Arg Arg Phe Leu Ser Ala Leu Ala Cys His Pro Asn 
225                 230                 235                 240 


Asn Phe Arg Ile Gly Ser Ser Ser Leu Val Pro Lys Leu Arg Lys Ile 
                245                 250                 255     


Ile Ala Gln Asn Pro Ala Val 
            260             


<210>  5
<211>  226
<212>  PRT
<213>  Pyrobaculum aerophilum

<400>  5

Met Ile His Leu Val Asp Tyr Ala Leu Leu Lys Pro Tyr Leu Thr Val 
1               5                   10                  15      


Asp Glu Ala Val Ala Gly Ala Arg Lys Ala Glu Glu Leu Gly Val Ala 
            20                  25                  30          


Ala Tyr Cys Val Asn Pro Ile Tyr Ala Pro Val Val Arg Pro Leu Leu 
        35                  40                  45              


Arg Lys Val Lys Leu Cys Val Val Ala Asp Phe Pro Phe Gly Ala Leu 
    50                  55                  60                  


Pro Thr Ala Ser Arg Ile Ala Leu Val Ser Arg Leu Ala Glu Val Ala 
65                  70                  75                  80  


Asp Glu Ile Asp Val Val Ala Pro Ile Gly Leu Val Lys Ser Arg Arg 
                85                  90                  95      


Trp Ala Glu Val Arg Arg Asp Leu Ile Ser Val Val Gly Ala Ala Gly 
            100                 105                 110         


Gly Arg Val Val Lys Val Ile Thr Glu Glu Pro Tyr Leu Arg Asp Glu 
        115                 120                 125             


Glu Arg Tyr Thr Leu Tyr Asp Ile Ile Ala Glu Ala Gly Ala His Phe 
    130                 135                 140                 


Ile Lys Ser Ser Thr Gly Phe Ala Glu Glu Ala Tyr Ala Ala Arg Gln 
145                 150                 155                 160 


Gly Asn Pro Val His Ser Thr Pro Glu Arg Ala Ala Ala Ile Ala Arg 
                165                 170                 175     


Tyr Ile Lys Glu Lys Gly Tyr Arg Leu Gly Val Lys Met Ala Gly Gly 
            180                 185                 190         


Ile Arg Thr Arg Glu Gln Ala Lys Ala Ile Val Asp Ala Ile Gly Trp 
        195                 200                 205             


Gly Glu Asp Pro Ala Arg Val Arg Leu Gly Thr Ser Thr Pro Glu Ala 
    210                 215                 220                 


Leu Leu 
225     


<210>  6
<211>  223
<212>  PRT
<213>  Geobacillus thermoglucosidasius

<400>  6

Met Glu Asn Asn Ile Ala Lys Met Ile Asp His Thr Leu Leu Lys Ala 
1               5                   10                  15      


Asp Thr Thr Lys Ala Gln Ile Val Lys Leu Cys Glu Glu Ala Lys Gln 
            20                  25                  30          


Tyr Gly Phe Ala Ser Val Cys Val Asn Pro Thr Trp Val Ala Thr Ala 
        35                  40                  45              


Ala Glu Leu Leu Lys Gly Thr Asp Val Lys Val Cys Thr Val Ile Gly 
    50                  55                  60                  


Phe Pro Leu Gly Ala Asn Thr Pro Glu Thr Lys Ala Phe Glu Thr Lys 
65                  70                  75                  80  


Asn Ala Ile Glu Asn Gly Ala Thr Glu Val Asp Met Val Ile Asn Ile 
                85                  90                  95      


Gly Ala Leu Lys Asp Gly Asn Asp Asp Leu Val Glu Arg Asp Ile Arg 
            100                 105                 110         


Ala Val Val Glu Ala Ala Lys Gly Lys Ala Leu Val Lys Val Ile Ile 
        115                 120                 125             


Glu Thr Cys Leu Leu Thr Glu Glu Glu Lys Val Arg Ala Cys Gln Leu 
    130                 135                 140                 


Ala Val Lys Ala Gly Ala Asp Tyr Val Lys Thr Ser Thr Gly Phe Ser 
145                 150                 155                 160 


Thr Gly Gly Ala Thr Thr Glu Asp Val Ala Leu Met Arg Lys Thr Val 
                165                 170                 175     


Gly Gln Asn Ile Gly Val Lys Ala Ser Gly Gly Val Arg Asp Ile Lys 
            180                 185                 190         


Ser Ala Met Ala Met Val Glu Ala Gly Ala Thr Arg Ile Gly Thr Ser 
        195                 200                 205             


Ser Gly Ala Ala Ile Val Ala Gly Lys Thr Ala Ala Ser Asp Tyr 
    210                 215                 220             


<210>  7
<211>  221
<212>  PRT
<213>  Acetobacterium woodii

<400>  7

Met Thr Asn Lys Glu Ile Leu Ser His Ile Asp His Thr Leu Leu Lys 
1               5                   10                  15      


Gln Phe Ala Thr Trp Glu Glu Ile Lys Val Ile Cys Glu Asp Ala Ile 
            20                  25                  30          


Glu Tyr Gln Thr Ala Ser Val Cys Ile Pro Pro Ala Tyr Val Lys Lys 
        35                  40                  45              


Ile Lys Lys Thr Tyr Gly Asp Lys Leu Asn Ile Cys Thr Val Ile Gly 
    50                  55                  60                  


Phe Pro Leu Gly Tyr Asn Thr Thr Ala Thr Lys Val Phe Glu Ala Val 
65                  70                  75                  80  


Asp Ala Val Lys Asn Gly Ala Ser Glu Ile Asp Met Val Ile Asn Ile 
                85                  90                  95      


Thr Ala Leu Lys Asn Lys Asp Tyr Asp Leu Val Gln Asn Glu Ile Ala 
            100                 105                 110         


Glu Ile Lys Lys Ala Ile Gly Ala Lys Ile Leu Lys Val Ile Val Glu 
        115                 120                 125             


Thr Cys Phe Leu Thr Ala Ala Glu Lys Val Lys Val Cys Glu Leu Val 
    130                 135                 140                 


Thr Asn Ala Gly Ala Asp Tyr Ile Lys Thr Ser Thr Gly Phe Gly Thr 
145                 150                 155                 160 


Ala Gly Ala Thr Ile Glu Asp Ile Arg Leu Phe Lys Ala His Ile Gly 
                165                 170                 175     


Pro Asn Val Lys Met Lys Ala Ala Gly Gly Val Lys Thr Val Asp Asp 
            180                 185                 190         


Leu Ile Ala Phe Ile Asn Glu Gly Cys Ala Arg Ile Gly Thr Ser Ser 
        195                 200                 205             


Ala Val Lys Leu Leu Lys Gly Glu Ala Val Ser Gly Tyr 
    210                 215                 220     


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Sequence

<400>  8
ggggatccaa tgatacacca atcagtgc                                          28


<210>  9
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  9
ggtctagatt gaacccgtct ccttacg                                           27


<210>  10
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  10
gggaattcat gtcgatcata ttgaagg                                           27


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  11
aaggatccct taaacgacca tcc                                               23


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  12
cctctagagt gagatgatgg ataac                                             25


<210>  13
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  13
gcaagcttct gcagtcatcc aaatgtgctt aacaacc                                37


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  14
aatctagatt agtcggtgcc tgtg                                              24


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  15
ggaagctttc tttacgttct acg                                               23


<210>  16
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  16
aaactagtgg cgattccaac ggcgat                                            26


<210>  17
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  17
aactcgagct acatctgaca gacttttttc gg                                     32


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  18
cgatttccga ttgattgctt                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  19
aatcccaaat gagccaacag                                                   20


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  20
ggactagttg ttatttggcg atcagc                                            26


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  21
ggctgcagcg cacccataca aagc                                              24


<210>  22
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  22
aacatcaaca tgcggccgca cttaccaaat tatctgcgtc g                           41


<210>  23
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  23
aatttggtaa gtgcggccgc atgttgatgt tattctcatg c                           41


<210>  24
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  24
gagtcgacgc agtatcttaa aattttgtat aataggaatt gaagttaaat tagatgctaa       60

aaatttgtaa ttaagaagga gtgattacat gttacgtcct gtagaaacc                  109


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  25
ttgcatgctc attgtttgcc tccc                                              24


<210>  26
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  26
agctttcgag cgcggaac                                                     18


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  27
gagttccatg tggtctacca tac                                               23


<210>  28
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  28
catggaggtt aaggctgagt tgacgataca ctgtc                                  35


<210>  29
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  29
gtcaactcag ccttaacctc catgacgacc ag                                     32


<210>  30
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  30
gacgagcaag gcaagaccgg gatccgacag taaccgtagg taccttcg                    48


<210>  31
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  31
ccagtgatct ctttatcgac ctcc                                              24


<210>  32
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  32
ggtgtgcatg tgcaaggaca cgcacctttt ctag                                   34


<210>  33
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  33
tgcgtgtcct tgcacatgca caccgatgag g                                      31


<210>  34
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  34
gacgagcaag gcaagaccgg gatccgcttc aacccaagct tgtagc                      46


<210>  35
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  35
gccatatggc atccgacacc atcc                                              24


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  36
ccggatcctc agtcccgtgc c                                                 21


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  37
gccatatgtc agttttcgtt tcagg                                             25


<210>  38
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  38
cggatcctta tattctgccc tc                                                22


<210>  39
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  39
aatcaacagg atattgaaca ggtggtg                                           27


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  40
gtagctgctg gcgctcttac                                                   20


<210>  41
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  41
atgacggtga atattgctaa aatgatcg                                          28


<210>  42
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence

<400>  42
ttaatagtca gcgccgccgg tttg                                              24


<210>  43
<211>  672
<212>  DNA
<213>  Geobacillus thermodenitrificans

<400>  43
atgacggtga atattgctaa aatgatcgat catacgttgc ttaagccaga agcgacggaa       60

gagcaaatca ttcaactatg cgacgaagca aagcaacacg gcttcgcctc ggtgtgcgtc      120

aacccagcgt gggtgaaaac agcggcacgc gagctttccg acactgatgt ccgcgtctgc      180

acggtcatcg gctttccgct tggggcgacg acgccggaaa caaaggcgtt tgaaacgaac      240

aacgctatcg aaaacggcgc ccgcgaagtc gatatggtaa tcaacatcgg cgcgttaaaa      300

agtggtaacg atgaactcgt tgagcgcgac attcgtgcgg ttgttgaggc ggcgtccggg      360

aaagcgcttg tgaaagtgat catcgaaacg gccttgttga ctgatgagga aaaagtgcgc      420

gcctgccaat tggcggtgaa agcgggcgcc gattacgtaa aaacgtcgac cggattctca      480

ggcggcggag cgacggtcga agacgtggcg ctgatgcgcc ggacagttgg cgataaagca      540

ggtgtcaaag cctcaggagg cgtccgcgac cgaaaaacag ccgaagcgat gattgaagct      600

ggggccacgc gcattgggac gagctccggg gtggcgatcg tcagcggcca aaccggcggc      660

gctgactatt aa                                                          672


