﻿               SEQUENCE LISTING

<110> ethris GmbH

<120> Novel minimal UTR sequences

<130> Y2004 PCT S3

<150> EP 16 16 3264.1
<151> 2016-03-31

<150> EP 16 17 7094.6
<151> 2016-06-30

<160> 45

<170> BiSSAP 1.2

<210> 1
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..7
<223> /mol_type="unassigned DNA"
      /note="Minmal UTR1"
      /organism="Artificial Sequence"

<400> 1
cgccacc                                                        7


<210> 2
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..8
<223> /mol_type="unassigned DNA"
      /note="Minimal UTR2"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 2
<223> /replace="A"
      /replace="T"
      /replace="C"
      /replace="G"

<400> 2
cngccacc                                                           8


<210> 3
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /mol_type="unassigned DNA"
      /note="T7 promoter"
      /organism="Artificial Sequence"

<400> 3
taatacgact cactataggg aga                                            23


<210> 4
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..23
<223> /mol_type="unassigned DNA"
      /note="T3 promoter"
      /organism="Artificial Sequence"

<400> 4
aattaaccct cactaaaggg aga                                            23


<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="SP6 promoter"
      /organism="Artificial Sequence"

<400> 5
atttaggtga cactatagaa g                                              21


<210> 6
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="K11 promoter"
      /organism="Artificial Sequence"

<400> 6
aattagggca cactataggg a                                              21


<210> 7
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..6
<223> /mol_type="unassigned DNA"
      /note="T7 promoter"
      /organism="Artificial Sequence"

<400> 7
gggaga                                                          6


<210> 8
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..6
<223> /mol_type="unassigned DNA"
      /note="T3 promoter"
      /organism="Artificial Sequence"

<400> 8
gggaga                                                        6


<210> 9
<211> 4
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4
<223> /mol_type="unassigned DNA"
      /note="SP6 promoter"
      /organism="Artificial Sequence"

<400> 9
gaag                                                           4


<210> 10
<211> 4
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..4
<223> /mol_type="unassigned DNA"
      /note="K11 promoter"
      /organism="Artificial Sequence"

<400> 10
ggga                                                           4


<210> 11
<211> 16
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..16
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR1"
      /organism="Artificial Sequence"

<400> 11
caugguggcg ucuccc                                                    16


<210> 12
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..17
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR2"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="G"
      /replace="C"
      /replace="U"
      /replace="A"

<400> 12
caugguggcn gucuccc                                                   17


<210> 13
<211> 16
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..16
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR1 including
      TISU element"
      /organism="Artificial Sequence"

<400> 13
caucuuggcg ucuccc                                                    16


<210> 14
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..17
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR2 including
      TISU element"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="G"
      /replace="C"
      /replace="A"
      /replace="U"

<400> 14
caucuuggcn gucuccc                                                   17


<210> 15
<211> 16
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..16
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR1"
      /organism="Artificial Sequence"

<400> 15
cauggcggcg ucuccc                                                    16


<210> 16
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..17
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR2"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="U"
      /replace="A"
      /replace="G"
      /replace="C"

<400> 16
cauggcggcn gucuccc                                                   17


<210> 17
<211> 16
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..16
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR1 including TISU element"
      /organism="Artificial Sequence"

<400> 17
caucucggcg ucuccc                                                    16


<210> 18
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..17
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR2 including TISU element"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="U"
      /replace="G"
      /replace="C"
      /replace="A"

<400> 18
caucucggcn gucuccc                                                   17


<210> 19
<211> 14
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR1"
      /organism="Artificial Sequence"

<400> 19
caugguggcg uccc                                                      14


<210> 20
<211> 15
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..15
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR2"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="A"
      /replace="C"
      /replace="G"
      /replace="U"

<400> 20
caugguggcn guccc                                                     15


<210> 21
<211> 14
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR1 including
      TISU element"
      /organism="Artificial Sequence"

<400> 21
caucuuggcg uccc                                                      14


<210> 22
<211> 15
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..15
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR2 including
      TISU element"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="A"
      /replace="C"
      /replace="G"
      /replace="U"

<400> 22
caucuuggcn guccc                                                     15


<210> 23
<211> 14
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR1"
      /organism="Artificial Sequence"

<400> 23
cauggcggcg uccc                                                      14


<210> 24
<211> 15
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..15
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR2"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="A"
      /replace="C"
      /replace="G"
      /replace="U"

<400> 24
cauggcggcn guccc                                                     15


<210> 25
<211> 14
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR1 including TISU element"
      /organism="Artificial Sequence"

<400> 25
caucucggcg uccc                                                      14


<210> 26
<211> 15
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..15
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR2 including TISU element"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="A"
      /replace="C"
      /replace="G"
      /replace="U"

<400> 26
caucucggcn guccc                                                     15


<210> 27
<211> 14
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR1"
      /organism="Artificial Sequence"

<400> 27
caugguggcg cuuc                                                      14


<210> 28
<211> 15
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..15
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR2"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="A"
      /replace="C"
      /replace="G"
      /replace="U"

<400> 28
caugguggcn gcuuc                                                       15


<210> 29
<211> 14
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR1 including
      TISU element"
      /organism="Artificial Sequence"

<400> 29
caucuuggcg cuuc                                                      14


<210> 30
<211> 15
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..15
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of minimal UTR2 including
      TISU element"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="A"
      /replace="C"
      /replace="G"
      /replace="U"

<400> 30
caucuuggcn gcuuc                                                     15


<210> 31
<211> 14
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR1"
      /organism="Artificial Sequence"

<400> 31
cauggcggcg cuuc                                                      14


<210> 32
<211> 15
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..15
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR2"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="A"
      /replace="C"
      /replace="G"
      /replace="U"

<400> 32
cauggcggcn gcuuc                                                      15


<210> 33
<211> 14
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..14
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR1 including TISU element"
      /organism="Artificial Sequence"

<400> 33
caucucggcg cuuc                                                      14


<210> 34
<211> 15
<212> RNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..15
<223> /mol_type="unassigned RNA"
      /note="Reverse complementary sequence of the alternative minimal
      UTR2 including TISU element"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 10
<223> /replace="A"
      /replace="C"
      /replace="G"
      /replace="U"

<400> 34
caucucggcn gcuuc                                                     15


<210> 35
<211> 637
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..637
<223> /mol_type="unassigned DNA"
      /note="T7-TISU-hEPO (codon optimized)"
      /organism="Artificial Sequence"

<400> 35
gtgactagat cttaatacga ctcactatag ggagactgcc aagatgggcg tgcacgaatg      60

tcctgcttgg ctgtggctgc tgctgagcct gctgtctctg cctctgggac tgcctgtgct     120

gggagcccct cctagactga tctgcgacag ccgggtgctg gaaagatacc tgctggaagc     180

caaagaggcc gagaacatca ccaccggctg cgccgagcac tgcagcctga acgagaatat     240

caccgtgccc gacaccaaag tgaacttcta cgcctggaag cggatggaag tgggccagca     300

ggctgtggaa gtgtggcagg gactggccct gctgagcgaa gctgtgctga gaggacaggc     360

tctgctcgtg aacagcagcc agccttggga gcctctgcag ctgcacgtgg acaaggccgt     420

gtctggcctg agaagcctga ccacactgct gagagccctg ggggcccaga aagaggccat     480

ctctccacct gatgccgcct ctgccgcccc tctgagaacc atcaccgccg acaccttcag     540

aaagctgttc cgggtgtaca gcaacttcct gcggggcaag ctgaagctgt acacaggcga     600

ggcctgccgg accggcgata gataattcga agtgact                              637


<210> 36
<211> 1120
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1120
<223> /mol_type="unassigned DNA"
      /note="T7-TISU-hOTC (codon optimized)"
      /organism="Artificial Sequence"

<400> 36
gtgactgcta gctaatacga ctcactatag ggagactgcc aagatgctgt tcaacctgcg      60

gatcctgctg aacaacgccg ccttccggaa cggccacaac ttcatggtgc gcaacttcag     120

atgcggccag cccctgcaga acaaggtgca gctgaagggc agggacctgc tgaccctgaa     180

gaacttcacc ggcgaagaga tcaagtacat gctgtggctg agcgccgacc tgaagttccg     240

gatcaagcag aagggcgagt acctgcccct gctgcagggc aagtctctgg gcatgatctt     300

cgagaagcgg agcacccgga cccggctgtc taccgagaca ggatttgccc tgctgggcgg     360

ccacccttgc tttctgacca cccaggatat ccacctgggc gtgaacgaga gcctgaccga     420

cacagccaga gtgctgagca gcatggccga tgccgtgctg gccagagtgt acaagcagag     480

cgacctggac accctggcca aagaggccag catccccatc atcaacggcc tgtccgacct     540

gtaccacccc atccagatcc tggccgacta cctgaccctg caggaacact acagctccct     600

gaagggcctg acactgagct ggatcggcga cggcaacaac atcctgcact ctatcatgat     660

gagcgccgcc aagttcggca tgcatctgca ggccgccacc cccaagggct atgagcctga     720

tgccagcgtg accaagctgg ccgagcagta cgccaaagag aacggcacca agctgctgct     780

gaccaacgac cctctggaag ccgcccacgg cggcaatgtg ctgatcaccg atacctggat     840

cagcatgggc caggaagagg aaaagaagaa gcggctgcag gccttccagg gctaccaagt     900

gaccatgaag accgccaaag tggccgccag cgactggacc ttcctgcact gcctgcccag     960

aaagcccgaa gaggtggacg acgaggtgtt ctacagcccc cggtccctgg tgtttcccga    1020

ggccgagaac cggaagtgga ccatcatggc tgtgatggtg tctctgctga ccgactactc    1080

cccccagctg cagaagccca agttctgaag cgctgtgact                          1120


<210> 37
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /mol_type="unassigned DNA"
      /note="T7Luc2"
      /organism="Artificial Sequence"

<400> 37
tatagggaga cgccaccatg                                                20


<210> 38
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..19
<223> /mol_type="unassigned DNA"
      /note="T7Luc2 (ΔC)"
      /organism="Artificial Sequence"

<400> 38
tatagggaga gccaccatg                                                 19


<210> 39
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="T7Luc2 (+8+A)"
      /organism="Artificial Sequence"

<400> 39
tatagggaga cagccaccat g                                              21


<210> 40
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="T7Luc2 (+8+T)"
      /organism="Artificial Sequence"

<400> 40
tatagggaga ctgccaccat g                                              21


<210> 41
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="T7Luc2 (+8+G)"
      /organism="Artificial Sequence"

<400> 41
tatagggaga cggccaccat g                                              21


<210> 42
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="T7Luc2 (+8+C)"
      /organism="Artificial Sequence"

<400> 42
tatagggaga ccgccaccat g                                              21


<210> 43
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="T7Luc2 (+8+Sp30)"
      /organism="Artificial Sequence"

<220> 
<221> variation
<222> 12
<223> /replace="sequence of a random sequence of 30 nucleotides"

<400> 43
tatagggaga cngccaccat g                                              21


<210> 44
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..50
<223> /mol_type="unassigned DNA"
      /note="T7Luc2 (+8+hAg)"
      /organism="Artificial Sequence"

<400> 44
tatagggaga ctcttctggt ccccacagac tcagagagaa cgccaccatg                50


<210> 45
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /mol_type="unassigned DNA"
      /note="T7Luc2 (+8+T)+TISU"
      /organism="Artificial Sequence"

<400> 45
tatagggaga ctgccaagat g                                              21


