                         SEQUENCE LISTING

<110>  GENIA TECHNOLOGIES, INC.
 
<120>  NANOPORE PROTEIN CONJUGATES AND USES THEREOF

<130>  P33379-WO

<140>
<141>

<150>  61/316,236
<151>  2016-03-31

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  1023
<212>  DNA
<213>  Staphylococcus aureus

<400>  1
atggcagatc tcgatcccgc gaaattaata cgactcacta tagggaggcc acaacggttt       60

ccctctagaa ataattttgt ttaactttaa gaaggagata tacaaatgga ttcagatatt      120

aatattaaaa caggtacaac agatattggt tcaaatacaa cagtaaaaac tggtgattta      180

gtaacttatg ataaagaaaa tggtatgcat aaaaaagtat tttattcttt tattgatgat      240

aaaaatcata ataaaaaatt gttagttatt cgtacaaaag gtactattgc aggtcaatat      300

agagtatata gtgaagaagg tgctaataaa agtggtttag catggccatc tgcttttaaa      360

gttcaattac aattacctga taatgaagta gcacaaattt cagattatta tccacgtaat      420

agtattgata caaaagaata tatgtcaaca ttaacttatg gttttaatgg taatgtaaca      480

ggtgatgata ctggtaaaat tggtggttta attggtgcta atgtttcaat tggtcataca      540

ttaaaatatg tacaaccaga ttttaaaaca attttagaaa gtcctactga taaaaaagtt      600

ggttggaaag taatttttaa taatatggtt aatcaaaatt ggggtcctta tgatcgtgat      660

agttggaatc ctgtatatgg taatcaatta tttatgaaaa caagaaatgg ttctatgaaa      720

gcagctgata atttcttaga tccaaataaa gcatcaagtt tattatcttc aggtttttct      780

cctgattttg caacagttat tactatggat agaaaagcat caaaacaaca aacaaatatt      840

gatgttattt atgaacgtgt aagagatgat tatcaattac attggacatc aactaattgg      900

aaaggtacaa atactaaaga taaatggaca gatagaagtt cagaaagata taaaattgat      960

tgggaaaaag aagaaatgac aaatggtctc agcgcttgga gccacccgca gttcgaaaaa     1020

taa                                                                   1023


<210>  2
<211>  64
<212>  PRT
<213>  Sulfolobus solfataricus

<400>  2

Met Ala Thr Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp 
1               5                   10                  15      


Ile Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe 
            20                  25                  30          


Thr Tyr Asp Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu 
        35                  40                  45              


Lys Asp Ala Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys 
    50                  55                  60                  


<210>  3
<211>  305
<212>  PRT
<213>  Staphylococcus aureus

<400>  3

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 
    290                 295                 300                 


Lys 
305 


<210>  4
<211>  293
<212>  PRT
<213>  Staphylococcus aureus

<400>  4

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  5
<211>  362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alpha-HL/Sso7d conjugate

<400>  5

Met Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly 
1               5                   10                  15      


Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu 
            20                  25                  30          


Asn Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn 
        35                  40                  45              


His Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly 
    50                  55                  60                  


Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala 
65                  70                  75                  80  


Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val 
                85                  90                  95      


Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu 
            100                 105                 110         


Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp 
        115                 120                 125             


Asp Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly 
    130                 135                 140                 


Ala Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser 
145                 150                 155                 160 


Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val 
                165                 170                 175     


Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr 
            180                 185                 190         


Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala 
        195                 200                 205             


Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly 
    210                 215                 220                 


Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser 
225                 230                 235                 240 


Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp 
                245                 250                 255     


Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys 
            260                 265                 270         


Asp Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu 
        275                 280                 285             


Lys Glu Glu Met Thr Asn Gly Leu Ser Ala Met Ala Thr Val Lys Phe 
    290                 295                 300                 


Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp Ile Ser Lys Ile Lys Lys 
305                 310                 315                 320 


Val Trp Arg Val Gly Lys Met Ile Ser Phe Thr Tyr Asp Glu Gly Gly 
                325                 330                 335     


Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys Asp Ala Pro Lys Glu 
            340                 345                 350         


Leu Leu Gln Met Leu Glu Lys Gln Lys Lys 
        355                 360         


<210>  6
<211>  1086
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  alpha-HL/Sso7d conjugate coding sequence

<400>  6
atggcagatt cagatattaa tattaaaaca ggtacaacag atattggttc aaatacaaca       60

gtaaaaactg gtgatttagt aacttatgat aaagaaaatg gtatgcataa aaaagtattt      120

tattctttta ttgatgataa aaatcataat aaaaaattgt tagttattcg tacaaaaggt      180

actattgcag gtcaatatag agtatatagt gaagaaggtg ctaataaaag tggtttagca      240

tggccatctg cttttaaagt tcaattacaa ttacctgata atgaagtagc acaaatttca      300

gattattatc cacgtaatag tattgataca aaagaatata tgtcaacatt aacttatggt      360

tttaatggta atgtaacagg tgatgatact ggtaaaattg gtggtttaat tggtgctaat      420

gtttcaattg gtgcgacatt aaaatatgta caaccagatt ttaaaacaat tttagaaagt      480

cctactgata aaaaagttgg ttggaaagta atttttaata atatggttaa tcaaaattgg      540

ggtccttatg atcgtgatag ttggaatcct gtatatggta atcaattatt tatgaaaaca      600

agaaatggtt ctatgaaagc agctgataat ttcttagatc caaataaagc atcaagttta      660

ttatcttcag gtttttctcc tgattttgca acagttatta ctatggatag aaaagcatca      720

aaacaacaaa caaatattga tgttatttat gaacgtgtaa gagatgatta tcaattacat      780

tggacatcaa ctaattggaa aggtacaaat actaaagata aatggacaga tagaagttca      840

gaaagatata aaattgattg ggaaaaagaa gaaatgacaa atggtctcag cgctatggct      900

accgttaaat tcaaatacaa aggtgaagaa aaagaagttg acatctctaa aatcaagaaa      960

gtgtggcgtg ttggtaaaat gatctctttc acctacgacg aaggtggtgg taaaaccggt     1020

cgtggtgctg tttctgaaaa agacgctccg aaagaactgc tgcagatgct ggaaaaacag     1080

aaaaaa                                                                1086


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GLSA linker

<400>  7

Gly Leu Ser Ala 
1               


<210>  8
<211>  305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N17K alpha-HL

<400>  8

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Lys Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 
    290                 295                 300                 


Lys 
305 


<210>  9
<211>  305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N17R alpha-HL

<400>  9

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Arg Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 
    290                 295                 300                 


Lys 
305 


<210>  10
<211>  305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T12K alpha-HL

<400>  10

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Lys Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 
    290                 295                 300                 


Lys 
305 


<210>  11
<211>  305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T12R alpha-HL

<400>  11

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Arg Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 
    290                 295                 300                 


Lys 
305 


<210>  12
<211>  1125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  alpha-HL/Sso7d coding sequence with His-Tag

<400>  12
atggcagatt cagatattaa tattaaaaca ggtacaacag atattggttc aaatacaaca       60

gtaaaaactg gtgatttagt aacttatgat aaagaaaatg gtatgcataa aaaagtattt      120

tattctttta ttgatgataa aaatcataat aaaaaattgt tagttattcg tacaaaaggt      180

actattgcag gtcaatatag agtatatagt gaagaaggtg ctaataaaag tggtttagca      240

tggccatctg cttttaaagt tcaattacaa ttacctgata atgaagtagc acaaatttca      300

gattattatc cacgtaatag tattgataca aaagaatata tgtcaacatt aacttatggt      360

tttaatggta atgtaacagg tgatgatact ggtaaaattg gtggtttaat tggtgctaat      420

gtttcaattg gtgcgacatt aaaatatgta caaccagatt ttaaaacaat tttagaaagt      480

cctactgata aaaaagttgg ttggaaagta atttttaata atatggttaa tcaaaattgg      540

ggtccttatg atcgtgatag ttggaatcct gtatatggta atcaattatt tatgaaaaca      600

agaaatggtt ctatgaaagc agctgataat ttcttagatc caaataaagc atcaagttta      660

ttatcttcag gtttttctcc tgattttgca acagttatta ctatggatag aaaagcatca      720

aaacaacaaa caaatattga tgttatttat gaacgtgtaa gagatgatta tcaattacat      780

tggacatcaa ctaattggaa aggtacaaat actaaagata aatggacaga tagaagttca      840

gaaagatata aaattgattg ggaaaaagaa gaaatgacaa atggtctcag cgctatggct      900

accgttaaat tcaaatacaa aggtgaagaa aaagaagttg acatctctaa aatcaagaaa      960

gtgtggcgtg ttggtaaaat gatctctttc acctacgacg aaggtggtgg taaaaccggt     1020

cgtggtgctg tttctgaaaa agacgctccg aaagaactgc tgcagatgct ggaaaaacag     1080

aaaaaagaaa acctgtattt ccagggtcat caccatcacc atcac                     1125


