                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  Improved Sortase

<130>  P33507-WO

<150>  EP16162916.7
<151>  2016-03-30

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  206
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Asp 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Lys Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  2
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S

<400>  2

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  3
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S-E106G

<400>  3

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Gly Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  4
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S-N107W

<400>  4

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Trp Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  5
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S-F144L

<400>  5

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Leu 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  6
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S-G167E

<400>  6

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  7
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S-N107W-F144L

<400>  7

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Trp Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Leu 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  8
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S-F144L-G167E

<400>  8

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Leu 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  9
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S-N107W-G167E

<400>  9

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Trp Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  10
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Staph. aureus Sortase A D160S-K196S-N107W-F144-L-G167E

<400>  10

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Trp Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Leu 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Ser 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  11
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A

<400>  11

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Asp Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Lys Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  12
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S

<400>  12

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  13
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S-E47G

<400>  13

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Gly Asn 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  14
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S-N48W

<400>  14

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Trp 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  15
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S-F85L

<400>  15

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Leu Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  16
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S-G108E

<400>  16

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  17
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S-N48W-F85L

<400>  17

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Trp 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Leu Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  18
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S-F85L-G108E

<400>  18

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Leu Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  19
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S-N48W-G108E

<400>  19

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Trp 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  20
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  shortened Staph. aureus Sortase A D101S-K137S-N48W-F85L-G108E

<400>  20

Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr 
1               5                   10                  15      


Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro 
            20                  25                  30          


Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Trp 
        35                  40                  45              


Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile 
    50                  55                  60                  


Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly 
65                  70                  75                  80  


Ser Met Val Tyr Leu Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met 
                85                  90                  95      


Thr Ser Ile Arg Ser Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu 
            100                 105                 110         


Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr 
        115                 120                 125             


Asn Glu Lys Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala Thr 
    130                 135                 140                 


Glu Val Lys 
145         


<210>  21
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase recognition motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X=any amino acid residue except P

<400>  21

Leu Pro Xaa Thr Gly 
1               5   


<210>  22
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase recognition motif

<400>  22

Leu Pro Glu Thr Gly 
1               5   


<210>  23
<211>  2
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase acceptor motif

<400>  23

Gly Gly 
1       


<210>  24
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase acceptor motif

<400>  24

Gly Gly Gly 
1           


<210>  25
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase acceptor motif

<400>  25

Cys Gly Gly 
1           


<210>  26
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase acceptor motif

<400>  26

Lys Gly Gly 
1           


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Arg-tag

<400>  27

Arg Arg Arg Arg Arg 
1               5   


<210>  28
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Arg-tag 2

<400>  28

Arg Arg Arg Arg Arg Arg 
1               5       


<210>  29
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-tag

<400>  29

His His His His His His 
1               5       


<210>  30
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  30

Lys Asp His Leu Ile His Asn Val His Lys Glu Phe His Ala His Ala 
1               5                   10                  15      


His Asn Lys 
            


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  31

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  32
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  32

Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr 
1               5                   10                  15      


Lys Asp Asp Asp Asp Lys 
            20          


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag#

<400>  33

Ala Trp Arg His Pro Gln Phe Gly Gly 
1               5                   


<210>  34
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  34

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  35
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  35

Met Asp Val Glu Ala Trp Leu Gly Ala Arg 
1               5                   10  


<210>  36
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  36

Met Asp Val Glu Ala Trp Leu Gly Ala Arg Val Pro Leu Val Glu Thr 
1               5                   10                  15      


<210>  37
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  37

Met Asp Glu Lys Thr Thr Gly Trp Arg Gly Gly His Val Val Glu Gly 
1               5                   10                  15      


Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg Leu Glu His His Pro 
            20                  25                  30          


Gln Gly Gln Arg Glu Pro 
        35              


<210>  38
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  38

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  39
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  39

Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
1               5                   10                  15  


<210>  40
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  40

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  41
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid tag

<400>  41

Pro Ala Thr Thr Thr Gly Ser Ser Pro Gly Pro Thr Gln Ser His Tyr 
1               5                   10                  15      


Gly Gln Cys Gly Gly Ile Gly Tyr Ser Gly Pro Thr Val Cys Ala Ser 
            20                  25                  30          


Gly Thr Thr Cys Gln Val Leu Asn Pro Tyr Tyr Ser Gln Cys Leu 
        35                  40                  45          


<210>  42
<211>  32
<212>  PRT
<213>  Butyrivibrio fibrisolvens

<400>  42

Met Asp Trp Asn Ala Asn Ile Ala Pro Gly Asn Ser Val Glu Phe Gly 
1               5                   10                  15      


Ile Gln Gly Ala Gly Ser Val Gly Asn Val Ile Asp Ile Thr Val Glu 
            20                  25                  30          


<210>  43
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chitin-binding-domain

<400>  43

Thr Asn Pro Gly Val Ser Ala Trp Gln Val Asn Thr Ala Tyr Thr Ala 
1               5                   10                  15      


Gly Gln Leu Val Thr Tyr Asn Gly Lys Thr Tyr Lys Cys Leu Gln Pro 
            20                  25                  30          


His Thr Ser Leu Ala Gly Trp Glu Pro Ser Asn Val Pro Ala Leu Trp 
        35                  40                  45              


Gln Leu Gln 
    50      


<210>  44
<211>  209
<212>  PRT
<213>  Chondrus crispus

<400>  44

Met Pro Glu Ile Lys Leu Thr Tyr Phe Asp Met Arg Gly Arg Ala Glu 
1               5                   10                  15      


Ala Ser Arg Leu Ala Leu Val Val Gly Glu Ile Pro Phe Glu Asp Glu 
            20                  25                  30          


Arg Val Val Phe Asp His Trp Lys Glu Ala Lys Pro Lys Thr Pro Tyr 
        35                  40                  45              


Ala Ala Leu Pro Met Leu Thr Val Asp Gly Met Gln Val Ala Gln Ser 
    50                  55                  60                  


Asp Ala Ile Leu Arg Tyr Cys Gly Lys Leu Ala Gly Leu Tyr Pro Ser 
65                  70                  75                  80  


Asp Pro Leu Glu Ala Ala Lys Val Asp Glu Val Gly Gly Val Ile Asp 
                85                  90                  95      


Asp Val Thr His Ala Met Tyr Arg Tyr Arg Gly Asp Asp Lys Asp Lys 
            100                 105                 110         


Leu Arg Glu Glu Arg Asp Lys Phe Ser Lys Val Asp Val Pro Arg Tyr 
        115                 120                 125             


Val Gly Ala Leu Glu Lys Arg Leu Glu Ala Phe Gly Asp Gly Pro Trp 
    130                 135                 140                 


Ala Val Gly Gly Asn Met Thr Ile Ala Asp Leu His Ile Cys His Leu 
145                 150                 155                 160 


Val Thr Asn Ile Arg Cys Gly Met Leu Asp Phe Val Asp Lys Asp Leu 
                165                 170                 175     


Leu Glu Gly Tyr Val Arg Ile Val Lys Ser Tyr Ser Ala Val Met Glu 
            180                 185                 190         


His Pro Lys Val Thr Glu Trp Tyr Glu Lys Lys Pro Val Lys Met Phe 
        195                 200                 205             


Ser 
    


<210>  45
<211>  396
<212>  PRT
<213>  Escherichia coli

<400>  45

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Lys Ile Glu Glu Gly Lys 
            20                  25                  30          


Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu 
        35                  40                  45              


Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu 
    50                  55                  60                  


His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly 
65                  70                  75                  80  


Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr 
                85                  90                  95      


Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln 
            100                 105                 110         


Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys 
        115                 120                 125             


Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn 
    130                 135                 140                 


Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala 
145                 150                 155                 160 


Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn 
                165                 170                 175     


Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly 
            180                 185                 190         


Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly 
        195                 200                 205             


Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu 
    210                 215                 220                 


Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu 
225                 230                 235                 240 


Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp 
                245                 250                 255     


Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val 
            260                 265                 270         


Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu 
        275                 280                 285             


Ser Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu 
    290                 295                 300                 


Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn 
305                 310                 315                 320 


Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu 
                325                 330                 335     


Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys 
            340                 345                 350         


Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala 
        355                 360                 365             


Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp 
    370                 375                 380                 


Glu Ala Leu Lys Asp Ala Gln Thr Arg Ile Thr Lys 
385                 390                 395     


<210>  46
<211>  206
<212>  PRT
<213>  Staphylococcus aureus

<400>  46

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Asp 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Glu Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Lys Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  47
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase recognition motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X=any amino acid residue except P

<400>  47

Leu Pro Xaa Thr 
1               


<210>  48
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase recognition motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X=D, E, A, N, Q, K, R

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X=A,G

<400>  48

Leu Pro Xaa Thr Xaa 
1               5   


<210>  49
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase recognition motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X=D, E, A, N, Q, K, R

<400>  49

Leu Pro Xaa Thr Ala 
1               5   


<210>  50
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase recognition motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X=D, E, A, N, Q, K, R

<400>  50

Leu Pro Xaa Thr Gly 
1               5   


<210>  51
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  51

Met Arg Gly Ser His His His His His His Gly Ser 
1               5                   10          


<210>  52
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated Sortase from US 9,267,127

<400>  52

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Arg Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu 
        35                  40                  45              


Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys 
65                  70                  75                  80  


Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Asn Val Lys Pro Thr Ala Val Glu Val Leu Asp 
            100                 105                 110         


Glu Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Glu Glu Thr Gly Val Trp Glu Lys Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys 
145             


