                         SEQUENCE LISTING

<110>  UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
       Wu, Xianghao
       Eiteman, Mark
 
<120>  GENETICALLY ENGINEERED MICROBES AND METHODS FOR PRODUCING 
       CITRAMALATE

<130>  235.02640201

<150>  62/311607
<151>  2016-03-22

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  491
<212>  PRT
<213>  Methanococcus jannaschii

<400>  1

Met Met Val Arg Ile Phe Asp Thr Thr Leu Arg Asp Gly Glu Gln Thr 
1               5                   10                  15      


Pro Gly Val Ser Leu Thr Pro Asn Asp Lys Leu Glu Ile Ala Lys Lys 
            20                  25                  30          


Leu Asp Glu Leu Gly Val Asp Val Ile Glu Ala Gly Ser Ala Ile Thr 
        35                  40                  45              


Ser Lys Gly Glu Arg Glu Gly Ile Lys Leu Ile Thr Lys Glu Gly Leu 
    50                  55                  60                  


Asn Ala Glu Ile Cys Ser Phe Val Arg Ala Leu Pro Val Asp Ile Asp 
65                  70                  75                  80  


Ala Ala Leu Glu Cys Asp Val Asp Ser Val His Leu Val Val Pro Thr 
                85                  90                  95      


Ser Pro Ile His Met Lys Tyr Lys Leu Arg Lys Thr Glu Asp Glu Val 
            100                 105                 110         


Leu Glu Thr Ala Leu Lys Ala Val Glu Tyr Ala Lys Glu His Gly Leu 
        115                 120                 125             


Ile Val Glu Leu Ser Ala Glu Asp Ala Thr Arg Ser Asp Val Asn Phe 
    130                 135                 140                 


Leu Ile Lys Leu Phe Asn Glu Gly Glu Lys Val Gly Ala Asp Arg Val 
145                 150                 155                 160 


Cys Val Cys Asp Thr Val Gly Val Leu Thr Pro Gln Lys Ser Gln Glu 
                165                 170                 175     


Leu Phe Lys Lys Ile Thr Glu Asn Val Asn Leu Pro Val Ser Val His 
            180                 185                 190         


Cys His Asn Asp Phe Gly Met Ala Thr Ala Asn Thr Cys Ser Ala Val 
        195                 200                 205             


Leu Gly Gly Ala Val Gln Cys His Val Thr Val Asn Gly Ile Gly Glu 
    210                 215                 220                 


Arg Ala Gly Asn Ala Ser Leu Glu Glu Val Val Ala Ala Leu Lys Ile 
225                 230                 235                 240 


Leu Tyr Gly Tyr Asp Thr Lys Ile Lys Met Glu Lys Leu Tyr Glu Val 
                245                 250                 255     


Ser Arg Ile Val Ser Arg Leu Met Lys Leu Pro Val Pro Pro Asn Lys 
            260                 265                 270         


Ala Ile Val Gly Asp Asn Ala Phe Ala His Glu Ala Gly Ile His Val 
        275                 280                 285             


Asp Gly Leu Ile Lys Asn Thr Glu Thr Tyr Glu Pro Ile Lys Pro Glu 
    290                 295                 300                 


Met Val Gly Asn Arg Arg Arg Ile Ile Leu Gly Lys His Ser Gly Arg 
305                 310                 315                 320 


Lys Ala Leu Lys Tyr Lys Leu Asp Leu Met Gly Ile Asn Val Ser Asp 
                325                 330                 335     


Glu Gln Leu Asn Lys Ile Tyr Glu Arg Val Lys Glu Phe Gly Asp Leu 
            340                 345                 350         


Gly Lys Tyr Ile Ser Asp Ala Asp Leu Leu Ala Ile Val Arg Glu Val 
        355                 360                 365             


Thr Gly Lys Leu Val Glu Glu Lys Ile Lys Leu Asp Glu Leu Thr Val 
    370                 375                 380                 


Val Ser Gly Asn Lys Ile Thr Pro Ile Ala Ser Val Lys Leu His Tyr 
385                 390                 395                 400 


Lys Gly Glu Asp Ile Thr Leu Ile Glu Thr Ala Tyr Gly Val Gly Pro 
                405                 410                 415     


Val Asp Ala Ala Ile Asn Ala Val Arg Lys Ala Ile Ser Gly Val Ala 
            420                 425                 430         


Asp Ile Lys Leu Val Glu Tyr Arg Val Glu Ala Ile Gly Gly Gly Thr 
        435                 440                 445             


Asp Ala Leu Ile Glu Val Val Val Lys Leu Arg Lys Gly Thr Glu Ile 
    450                 455                 460                 


Val Glu Val Arg Lys Ser Asp Ala Asp Ile Ile Arg Ala Ser Val Asp 
465                 470                 475                 480 


Ala Val Met Glu Gly Ile Asn Met Leu Leu Asn 
                485                 490     


<210>  2
<211>  528
<212>  PRT
<213>  Geobacter sulfurreducens

<400>  2

Met Ser Leu Val Lys Leu Tyr Asp Thr Thr Leu Arg Asp Gly Thr Gln 
1               5                   10                  15      


Ala Glu Asp Ile Ser Phe Leu Val Glu Asp Lys Ile Arg Ile Ala His 
            20                  25                  30          


Lys Leu Asp Glu Ile Gly Ile His Tyr Ile Glu Gly Gly Trp Pro Gly 
        35                  40                  45              


Ser Asn Pro Lys Asp Val Ala Phe Phe Lys Asp Ile Lys Lys Glu Lys 
    50                  55                  60                  


Leu Ser Gln Ala Lys Ile Ala Ala Phe Gly Ser Thr Arg Arg Ala Lys 
65                  70                  75                  80  


Val Thr Pro Asp Lys Asp His Asn Leu Lys Thr Leu Ile Gln Ala Glu 
                85                  90                  95      


Pro Asp Val Cys Thr Ile Phe Gly Lys Thr Trp Asp Phe His Val His 
            100                 105                 110         


Glu Ala Leu Arg Ile Ser Leu Glu Glu Asn Leu Glu Leu Ile Phe Asp 
        115                 120                 125             


Ser Leu Glu Tyr Leu Lys Ala Asn Val Pro Glu Val Phe Tyr Asp Ala 
    130                 135                 140                 


Glu His Phe Phe Asp Gly Tyr Lys Ala Asn Pro Asp Tyr Ala Ile Lys 
145                 150                 155                 160 


Thr Leu Lys Ala Ala Gln Asp Ala Lys Ala Asp Cys Ile Val Leu Cys 
                165                 170                 175     


Asp Thr Asn Gly Gly Thr Met Pro Phe Glu Leu Val Glu Ile Ile Arg 
            180                 185                 190         


Glu Val Arg Lys His Ile Thr Ala Pro Leu Gly Ile His Thr His Asn 
        195                 200                 205             


Asp Ser Glu Cys Ala Val Ala Asn Ser Leu His Ala Val Ser Glu Gly 
    210                 215                 220                 


Ile Val Gln Val Gln Gly Thr Ile Asn Gly Phe Gly Glu Arg Cys Gly 
225                 230                 235                 240 


Asn Ala Asn Leu Cys Ser Ile Ile Pro Ala Leu Lys Leu Lys Met Lys 
                245                 250                 255     


Arg Glu Cys Ile Gly Asp Asp Gln Leu Arg Lys Leu Arg Asp Leu Ser 
            260                 265                 270         


Arg Phe Val Tyr Glu Leu Ala Asn Leu Ser Pro Asn Lys His Gln Ala 
        275                 280                 285             


Tyr Val Gly Asn Ser Ala Phe Ala His Lys Gly Gly Val His Val Ser 
    290                 295                 300                 


Ala Ile Gln Arg His Pro Glu Thr Tyr Glu His Leu Arg Pro Glu Leu 
305                 310                 315                 320 


Val Gly Asn Met Thr Arg Val Leu Val Ser Asp Leu Ser Gly Arg Ser 
                325                 330                 335     


Asn Ile Leu Ala Lys Ala Glu Glu Phe Asn Ile Lys Met Asp Ser Lys 
            340                 345                 350         


Asp Pro Val Thr Leu Glu Ile Leu Glu Asn Ile Lys Glu Met Glu Asn 
        355                 360                 365             


Arg Gly Tyr Gln Phe Glu Gly Ala Glu Ala Ser Phe Glu Leu Leu Met 
    370                 375                 380                 


Lys Arg Ala Leu Gly Thr His Arg Lys Phe Phe Ser Val Ile Gly Phe 
385                 390                 395                 400 


Arg Val Ile Asp Glu Lys Arg His Glu Asp Gln Lys Pro Leu Ser Glu 
                405                 410                 415     


Ala Thr Ile Met Val Lys Val Gly Gly Lys Ile Glu His Thr Ala Ala 
            420                 425                 430         


Glu Gly Asn Gly Pro Val Asn Ala Leu Asp Asn Ala Leu Arg Lys Ala 
        435                 440                 445             


Leu Glu Lys Phe Tyr Pro Arg Leu Lys Glu Val Lys Leu Leu Asp Tyr 
    450                 455                 460                 


Lys Val Arg Val Leu Pro Ala Gly Gln Gly Thr Ala Ser Ser Ile Arg 
465                 470                 475                 480 


Val Leu Ile Glu Ser Gly Asp Lys Glu Ser Arg Trp Gly Thr Val Gly 
                485                 490                 495     


Val Ser Glu Asn Ile Val Asp Ala Ser Tyr Gln Ala Leu Leu Asp Ser 
            500                 505                 510         


Val Glu Tyr Lys Leu His Lys Ser Glu Glu Ile Glu Gly Ser Lys Lys 
        515                 520                 525             


<210>  3
<211>  520
<212>  PRT
<213>  Thermoanaerobacter sp

<400>  3

Met Arg Asn Ile Arg Ile Tyr Asp Thr Thr Leu Arg Asp Gly Val Gln 
1               5                   10                  15      


Gly Gln Gly Ile Ser Phe Thr Val Glu Asp Lys Leu Lys Ile Val Lys 
            20                  25                  30          


Val Leu Asp Glu Phe Gly Val Ala Tyr Ile Glu Ala Gly Asn Pro Gly 
        35                  40                  45              


Ser Asn Pro Lys Asp Ile Glu Phe Phe Glu Arg Val Lys Asn Ile Lys 
    50                  55                  60                  


Leu Lys Asn Ala Lys Leu Ile Ala Phe Gly Ser Thr Arg Arg Ala Asn 
65                  70                  75                  80  


Thr Thr Thr Glu Glu Asp Ala Asn Val Ile Ser Leu Leu Asn Ala Asp 
                85                  90                  95      


Thr Glu Val Val Thr Ile Phe Gly Lys Ser Trp Asp Phe Gln Val Thr 
            100                 105                 110         


Glu Ile Leu Lys Thr Thr Leu Glu Glu Asn Leu Lys Met Ile Tyr Asp 
        115                 120                 125             


Thr Val Lys Phe Phe Lys Asp Lys Gly Lys Ser Val Ile Phe Asp Ala 
    130                 135                 140                 


Glu His Phe Phe Asp Gly Tyr Lys Gln Asn Pro Glu Tyr Ala Leu Lys 
145                 150                 155                 160 


Thr Leu Glu Val Ala Leu Glu Ala Gly Val Asp Ser Val Cys Leu Cys 
                165                 170                 175     


Asp Thr Lys Gly Gly Ala Phe Pro Met Glu Val Tyr Asp Ile Thr Lys 
            180                 185                 190         


Thr Val Val Asp Lys Phe Asn Thr Glu Val Gly Ile His Cys His Asn 
        195                 200                 205             


Asp Asn Gly Met Ala Val Ala Asp Ser Ile Met Ala Val Gln Ala Gly 
    210                 215                 220                 


Ala Ile Gln Leu Gln Gly Thr Ile Asn Gly Tyr Gly Glu Arg Cys Gly 
225                 230                 235                 240 


Asn Ala Asn Leu Cys Thr Leu Ile Pro Asn Leu Gln Leu Leu Met Gly 
                245                 250                 255     


Tyr Lys Cys Val Pro Asp Glu Asn Leu Lys Gln Leu Thr His Leu Ala 
            260                 265                 270         


Arg Phe Val Ser Glu Ile Ala Asn Val Thr Tyr Asp Glu Arg Ala Pro 
        275                 280                 285             


Tyr Val Gly Lys Asn Ala Phe Ser His Lys Ala Gly Met His Ala Asp 
    290                 295                 300                 


Ala Val Asn Lys Asn Thr Tyr Ser Tyr Glu Leu Ile Asp Pro Ser Leu 
305                 310                 315                 320 


Val Gly Asn Ser Arg Thr Phe Leu Ile Ser Glu Val Ala Gly Arg Gly 
                325                 330                 335     


Ala Val Leu Asn Ala Ile Asn Glu Ile Asp Pro Thr Ile Thr Lys Asp 
            340                 345                 350         


Ser Pro Glu Thr Lys Leu Ile Leu Asp Lys Leu Lys Glu Met Glu Tyr 
        355                 360                 365             


Leu Gly Tyr Gln Tyr Glu Asn Ala Gly Gly Ser Leu Glu Leu Leu Ile 
    370                 375                 380                 


Arg Lys Val Leu Gly Lys Tyr Lys Pro Ala Phe Asn Leu Lys Glu Phe 
385                 390                 395                 400 


Lys Val Ile Val Asn Glu Pro Ser Val Asn Ser Val Asn Ser Ser Ala 
                405                 410                 415     


Leu Ile Lys Val Glu Val Asp Ser Ile Glu Glu Ile Ala Ala Ala Glu 
            420                 425                 430         


Gly Asp Gly Pro Val His Ala Leu Asp Asn Ala Val Arg Arg Val Leu 
        435                 440                 445             


Glu Arg Phe Tyr Pro Gln Ile Lys Glu Met Arg Leu Thr Asp Tyr Lys 
    450                 455                 460                 


Val Arg Val Leu Asp Ser Asn Ser Ala Thr Ala Ala Lys Val Arg Val 
465                 470                 475                 480 


Ile Ile Glu Ser Thr Asp Gly Lys Asp Ser Trp Ser Thr Ile Gly Val 
                485                 490                 495     


Ser Thr Asp Ile Ile Glu Ala Ser Trp Arg Ala Leu Val Asp Ser Ile 
            500                 505                 510         


Glu Tyr Lys Leu Asn Lys Glu Ser 
        515                 520 


<210>  4
<211>  373
<212>  PRT
<213>  Artificial

<220>
<223>  citramalate synthase enzyme CimA3.7

<400>  4

Met Met Val Arg Ile Phe Asp Thr Thr Leu Arg Asp Gly Glu Gln Thr 
1               5                   10                  15      


Pro Gly Val Ser Leu Thr Pro Asn Asp Lys Leu Glu Ile Ala Lys Lys 
            20                  25                  30          


Leu Asp Glu Leu Gly Val Asp Val Ile Glu Ala Gly Ser Ala Val Thr 
        35                  40                  45              


Ser Lys Gly Glu Arg Glu Gly Ile Lys Leu Ile Thr Lys Glu Gly Leu 
    50                  55                  60                  


Asn Ala Glu Ile Cys Ser Phe Val Arg Ala Leu Pro Val Asp Ile Asp 
65                  70                  75                  80  


Ala Ala Leu Glu Cys Asp Val Asp Ser Val His Leu Val Val Pro Thr 
                85                  90                  95      


Ser Pro Ile His Met Lys Tyr Lys Leu Arg Lys Thr Glu Asp Glu Val 
            100                 105                 110         


Leu Val Thr Ala Leu Lys Ala Val Glu Tyr Ala Lys Glu Gln Gly Leu 
        115                 120                 125             


Ile Val Glu Leu Ser Ala Glu Asp Ala Thr Arg Ser Asp Val Asn Phe 
    130                 135                 140                 


Leu Ile Lys Leu Phe Asn Glu Gly Glu Lys Val Gly Ala Asp Arg Val 
145                 150                 155                 160 


Cys Val Cys Asp Thr Val Gly Val Leu Thr Pro Gln Lys Ser Gln Glu 
                165                 170                 175     


Leu Phe Lys Lys Ile Thr Glu Asn Val Asn Leu Pro Val Ser Val His 
            180                 185                 190         


Cys His Asn Asp Phe Gly Met Ala Thr Ala Asn Ala Cys Ser Ala Val 
        195                 200                 205             


Leu Gly Gly Ala Val Gln Cys His Val Thr Val Asn Gly Ile Gly Glu 
    210                 215                 220                 


Arg Ala Gly Asn Ala Ser Leu Glu Glu Val Val Ala Ala Ser Lys Ile 
225                 230                 235                 240 


Leu Tyr Gly Tyr Asp Thr Lys Ile Lys Met Glu Lys Leu Tyr Glu Val 
                245                 250                 255     


Ser Arg Ile Val Ser Arg Leu Met Lys Leu Pro Val Pro Pro Asn Lys 
            260                 265                 270         


Ala Ile Val Gly Asp Asn Ala Phe Ala His Glu Ala Gly Ile His Val 
        275                 280                 285             


Asp Gly Leu Ile Lys Asn Thr Glu Thr Tyr Glu Pro Ile Lys Pro Glu 
    290                 295                 300                 


Met Val Gly Asn Arg Arg Arg Ile Ile Leu Gly Lys His Ser Gly Arg 
305                 310                 315                 320 


Lys Ala Leu Lys Tyr Lys Leu Asp Leu Met Gly Ile Asn Val Ser Asp 
                325                 330                 335     


Glu Gln Leu Asn Lys Ile Tyr Glu Arg Val Lys Glu Phe Gly Asp Leu 
            340                 345                 350         


Gly Lys Tyr Ile Ser Asp Ala Asp Leu Leu Ala Ile Val Arg Glu Val 
        355                 360                 365             


Thr Gly Lys Leu Val 
    370             


<210>  5
<211>  426
<212>  PRT
<213>  Escherichia coli

<400>  5

Ala Asp Thr Lys Ala Lys Leu Thr Leu Asn Gly Asp Thr Ala Val Glu 
1               5                   10                  15      


Leu Asp Val Leu Lys Gly Thr Leu Gly Gln Asp Val Ile Asp Ile Arg 
            20                  25                  30          


Thr Leu Gly Ser Lys Gly Val Phe Thr Phe Asp Pro Gly Phe Thr Ser 
        35                  40                  45              


Thr Ala Ser Cys Glu Ser Lys Ile Thr Phe Ile Asp Gly Asp Glu Gly 
    50                  55                  60                  


Ile Leu Leu His Arg Gly Phe Pro Ile Asp Gln Leu Ala Thr Asp Ser 
65                  70                  75                  80  


Asn Tyr Leu Glu Val Cys Tyr Ile Leu Leu Asn Gly Glu Lys Pro Thr 
                85                  90                  95      


Gln Glu Gln Tyr Asp Glu Phe Lys Thr Thr Val Thr Arg His Thr Met 
            100                 105                 110         


Ile His Glu Gln Ile Thr Arg Leu Phe His Ala Phe Arg Arg Asp Ser 
        115                 120                 125             


His Pro Met Ala Val Met Cys Gly Ile Thr Gly Ala Leu Ala Ala Phe 
    130                 135                 140                 


Tyr His Asp Ser Leu Asp Val Asn Asn Pro Arg His Arg Glu Ile Ala 
145                 150                 155                 160 


Ala Phe Arg Leu Leu Ser Lys Met Pro Thr Met Ala Ala Met Cys Tyr 
                165                 170                 175     


Lys Tyr Ser Ile Gly Gln Pro Phe Val Tyr Pro Arg Asn Asp Leu Ser 
            180                 185                 190         


Tyr Ala Gly Asn Phe Leu Asn Met Met Phe Ser Thr Pro Cys Glu Pro 
        195                 200                 205             


Tyr Glu Val Asn Pro Ile Leu Glu Arg Ala Met Asp Arg Ile Leu Ile 
    210                 215                 220                 


Leu His Ala Asp His Glu Gln Asn Ala Ser Thr Ser Thr Val Arg Thr 
225                 230                 235                 240 


Ala Gly Ser Ser Gly Ala Asn Pro Phe Ala Cys Ile Ala Ala Gly Ile 
                245                 250                 255     


Ala Ser Leu Trp Gly Pro Ala His Gly Gly Ala Asn Glu Ala Ala Leu 
            260                 265                 270         


Lys Met Leu Glu Glu Ile Ser Ser Val Lys His Ile Pro Glu Phe Val 
        275                 280                 285             


Arg Arg Ala Lys Asp Lys Asn Asp Ser Phe Arg Leu Met Gly Phe Gly 
    290                 295                 300                 


His Arg Val Tyr Lys Asn Tyr Asp Pro Arg Ala Thr Val Met Arg Glu 
305                 310                 315                 320 


Thr Cys His Glu Val Leu Lys Glu Leu Gly Thr Lys Asp Asp Leu Leu 
                325                 330                 335     


Glu Val Ala Met Glu Leu Glu Asn Ile Ala Leu Asn Asp Pro Tyr Phe 
            340                 345                 350         


Ile Glu Lys Lys Leu Tyr Pro Asn Val Asp Phe Tyr Ser Gly Ile Ile 
        355                 360                 365             


Leu Lys Ala Met Gly Ile Pro Ser Ser Met Phe Thr Val Ile Phe Ala 
    370                 375                 380                 


Met Ala Arg Thr Val Gly Trp Ile Ala His Trp Ser Glu Met His Ser 
385                 390                 395                 400 


Asp Gly Met Lys Ile Ala Arg Pro Arg Gln Leu Tyr Thr Gly Tyr Glu 
                405                 410                 415     


Lys Arg Asp Phe Lys Ser Asp Ile Lys Arg 
            420                 425     


<210>  6
<211>  427
<212>  PRT
<213>  Klebsiella pneumoniae

<400>  6

Met Ser Asp Ala Lys Ala Lys Ile Thr Leu Gly Gly Asp Thr Ala Ile 
1               5                   10                  15      


Glu Leu Asp Val Leu Lys Gly Thr Leu Gly Gln Asp Val Ile Asp Ile 
            20                  25                  30          


Arg Ser Leu Gly Ser Lys Gly Val Phe Thr Phe Asp Pro Gly Phe Thr 
        35                  40                  45              


Ser Thr Ala Ser Cys Glu Ser Lys Ile Thr Phe Ile Asp Gly Asp Glu 
    50                  55                  60                  


Gly Ile Leu Leu His Arg Gly Phe Pro Ile Asp Gln Leu Ala Thr Glu 
65                  70                  75                  80  


Ser Asn Tyr Leu Glu Val Cys Tyr Ile Leu Leu Tyr Gly Glu Lys Pro 
                85                  90                  95      


Thr Gln Ala Glu Tyr Asp Glu Phe Lys Thr Thr Val Thr Arg His Thr 
            100                 105                 110         


Met Ile His Glu Gln Ile Thr Arg Leu Phe His Ala Phe Arg Arg Asp 
        115                 120                 125             


Ser His Pro Met Ala Val Met Cys Gly Ile Thr Gly Ala Leu Ala Ala 
    130                 135                 140                 


Phe Tyr His Asp Ser Leu Asp Val Asn Asn Pro Arg His Arg Glu Ile 
145                 150                 155                 160 


Ala Ala Tyr Arg Leu Leu Ser Lys Met Pro Thr Met Ala Ala Met Cys 
                165                 170                 175     


Tyr Lys Tyr Ser Ile Gly Gln Pro Phe Val Tyr Pro Arg Asn Asp Leu 
            180                 185                 190         


Ser Tyr Ala Gly Asn Phe Leu Arg Met Met Phe Ala Thr Pro Cys Glu 
        195                 200                 205             


Glu Tyr Glu Val Asn Pro Val Leu Glu Arg Ala Met Asp Arg Ile Leu 
    210                 215                 220                 


Ile Leu His Ala Asp His Glu Gln Asn Ala Ser Thr Ser Thr Val Arg 
225                 230                 235                 240 


Thr Ala Gly Ser Ser Gly Ala Asn Pro Phe Ala Cys Ile Ala Ala Gly 
                245                 250                 255     


Ile Ala Ser Leu Trp Gly Pro Ala His Gly Gly Ala Asn Glu Ala Ala 
            260                 265                 270         


Leu Lys Met Leu Glu Glu Ile Ser Ser Val Glu His Ile Pro Glu Phe 
        275                 280                 285             


Val Arg Arg Ala Lys Asp Lys Asn Asp Ser Phe Arg Leu Met Gly Phe 
    290                 295                 300                 


Gly His Arg Val Tyr Lys Asn Tyr Asp Pro Arg Ala Thr Val Met Arg 
305                 310                 315                 320 


Glu Thr Cys His Glu Val Leu Lys Glu Leu Gly Thr Lys Asp Asp Leu 
                325                 330                 335     


Leu Gln Val Ala Met Glu Leu Glu His Ile Ala Leu Asn Asp Pro Tyr 
            340                 345                 350         


Phe Ile Glu Lys Lys Leu Tyr Pro Asn Val Asp Phe Tyr Ser Gly Ile 
        355                 360                 365             


Ile Leu Lys Ala Met Gly Ile Pro Ser Ser Met Phe Thr Val Ile Phe 
    370                 375                 380                 


Ala Met Ala Arg Thr Val Gly Trp Ile Ala His Trp Asn Glu Met His 
385                 390                 395                 400 


Ser Asp Gly Met Lys Ile Ala Arg Pro Arg Gln Leu Tyr Thr Gly Tyr 
                405                 410                 415     


Ala Lys Arg Asp Phe Gln Ser Asp Ile Lys Arg 
            420                 425         


<210>  7
<211>  429
<212>  PRT
<213>  Pseudomonas putida

<400>  7

Met Ala Asp Lys Lys Ala Gln Leu Val Ile Glu Gly Ala Ala Pro Val 
1               5                   10                  15      


Glu Leu Pro Ile Leu Thr Gly Thr Val Gly Pro Asp Val Ile Asp Val 
            20                  25                  30          


Arg Gly Leu Gly Ala Thr Gly His Phe Thr Phe Asp Pro Gly Phe Met 
        35                  40                  45              


Ala Thr Ala Ser Cys Glu Ser Lys Ile Thr Tyr Ile Asp Gly Asp Lys 
    50                  55                  60                  


Gly Ile Leu Leu His Arg Gly Tyr Pro Ile Glu Gln Leu Ala Glu Gln 
65                  70                  75                  80  


Ser Asp Tyr Leu Glu Thr Cys Tyr Leu Leu Leu Asn Gly Glu Leu Pro 
                85                  90                  95      


Asn Ala Glu Gln Lys Ala Gln Phe Val Ser Thr Val Lys Asn His Thr 
            100                 105                 110         


Met Val His Glu Gln Leu Lys Ser Phe Phe Asn Gly Phe Arg Arg Asp 
        115                 120                 125             


Ala His Pro Met Ala Val Met Cys Gly Val Val Gly Ala Leu Ser Ala 
    130                 135                 140                 


Phe Tyr His Asp Ser Leu Asp Ile Asn Asn Pro Gln His Arg Glu Ile 
145                 150                 155                 160 


Ser Ala Val Arg Leu Val Ala Lys Met Pro Thr Leu Ala Ala Met Val 
                165                 170                 175     


Tyr Lys Tyr Ser Met Gly Gln Pro Met Met Tyr Pro Arg Asn Asp Leu 
            180                 185                 190         


Ser Tyr Ala Glu Asn Phe Leu His Met Met Phe Asn Thr Pro Cys Glu 
        195                 200                 205             


Ile Lys Pro Ile Ser Pro Val Leu Ala Lys Ala Met Asp Arg Ile Phe 
    210                 215                 220                 


Ile Leu His Ala Asp His Glu Gln Asn Ala Ser Thr Ser Thr Val Arg 
225                 230                 235                 240 


Leu Ala Gly Ser Ser Gly Ala Asn Pro Phe Ala Cys Ile Ala Ala Gly 
                245                 250                 255     


Ile Ala Ala Leu Trp Gly Pro Ala His Gly Gly Ala Asn Glu Ala Val 
            260                 265                 270         


Leu Thr Met Leu Asp Glu Ile Gly Asp Val Ser Asn Ile Asp Lys Phe 
        275                 280                 285             


Ile Ala Lys Ala Lys Asp Lys Asn Asp Pro Phe Lys Leu Met Gly Phe 
    290                 295                 300                 


Gly His Arg Val Tyr Lys Asn Arg Asp Pro Arg Ala Thr Val Met Lys 
305                 310                 315                 320 


Gln Thr Cys Asp Glu Val Leu Arg Glu Leu Gly Ile Lys Asn Asp Pro 
                325                 330                 335     


Gln Leu Glu Leu Ala Met Arg Leu Glu Glu Ile Ala Leu Thr Asp Pro 
            340                 345                 350         


Tyr Phe Ile Glu Arg Ser Leu Tyr Pro Asn Val Asp Phe Tyr Ser Gly 
        355                 360                 365             


Ile Ile Leu Lys Ala Ile Gly Ile Pro Thr Ser Met Phe Thr Val Ile 
    370                 375                 380                 


Phe Ala Leu Ala Arg Thr Val Gly Trp Ile Ser His Trp Lys Glu Met 
385                 390                 395                 400 


Leu Ser Ser Pro Tyr Lys Ile Gly Arg Pro Arg Gln Leu Tyr Thr Gly 
                405                 410                 415     


Glu Gln Lys Arg Asp Ile Val Ala Leu Lys Asp Arg Lys 
            420                 425                 


<210>  8
<211>  437
<212>  PRT
<213>  Corynebacterium glutamicum

<400>  8

Met Phe Glu Arg Asp Ile Val Ala Thr Asp Asn Asn Lys Ala Val Leu 
1               5                   10                  15      


His Tyr Pro Gly Gly Glu Phe Glu Met Asp Ile Ile Glu Ala Ser Glu 
            20                  25                  30          


Gly Asn Asn Gly Val Val Leu Gly Lys Met Leu Ser Glu Thr Gly Leu 
        35                  40                  45              


Ile Thr Phe Asp Pro Gly Tyr Val Ser Thr Gly Ser Thr Glu Ser Lys 
    50                  55                  60                  


Ile Thr Tyr Ile Asp Gly Asp Ala Gly Ile Leu Arg Tyr Arg Gly Tyr 
65                  70                  75                  80  


Asp Ile Ala Asp Leu Ala Glu Asn Ala Thr Phe Asn Glu Val Ser Tyr 
                85                  90                  95      


Leu Leu Ile Asn Gly Glu Leu Pro Thr Pro Asp Glu Leu His Lys Phe 
            100                 105                 110         


Asn Asp Glu Ile Arg His His Thr Leu Leu Asp Glu Asp Phe Lys Ser 
        115                 120                 125             


Gln Phe Asn Val Phe Pro Arg Asp Ala His Pro Met Ala Thr Leu Ala 
    130                 135                 140                 


Ser Ser Val Asn Ile Leu Ser Thr Tyr Tyr Gln Asp Gln Leu Asn Pro 
145                 150                 155                 160 


Leu Asp Glu Ala Gln Leu Asp Lys Ala Thr Val Arg Leu Met Ala Lys 
                165                 170                 175     


Val Pro Met Leu Ala Ala Tyr Ala His Arg Ala Arg Lys Gly Ala Pro 
            180                 185                 190         


Tyr Met Tyr Pro Asp Asn Ser Leu Asn Ala Arg Glu Asn Phe Leu Arg 
        195                 200                 205             


Met Met Phe Gly Tyr Pro Thr Glu Pro Tyr Glu Ile Asp Pro Ile Met 
    210                 215                 220                 


Val Lys Ala Leu Asp Lys Leu Leu Ile Leu His Ala Asp His Glu Gln 
225                 230                 235                 240 


Asn Cys Ser Thr Ser Thr Val Arg Met Ile Gly Ser Ala Gln Ala Asn 
                245                 250                 255     


Met Phe Val Ser Ile Ala Gly Gly Ile Asn Ala Leu Ser Gly Pro Leu 
            260                 265                 270         


His Gly Gly Ala Asn Gln Ala Val Leu Glu Met Leu Glu Asp Ile Lys 
        275                 280                 285             


Ser Asn His Gly Gly Asp Ala Thr Glu Phe Met Asn Lys Val Lys Asn 
    290                 295                 300                 


Lys Glu Asp Gly Val Arg Leu Met Gly Phe Gly His Arg Val Tyr Lys 
305                 310                 315                 320 


Asn Tyr Asp Pro Arg Ala Ala Ile Val Lys Glu Thr Ala His Glu Ile 
                325                 330                 335     


Leu Glu His Leu Gly Gly Asp Asp Leu Leu Asp Leu Ala Ile Lys Leu 
            340                 345                 350         


Glu Glu Ile Ala Leu Ala Asp Asp Tyr Phe Ile Ser Arg Lys Leu Tyr 
        355                 360                 365             


Pro Asn Val Asp Phe Tyr Thr Gly Leu Ile Tyr Arg Ala Met Gly Phe 
    370                 375                 380                 


Pro Thr Asp Phe Phe Thr Val Leu Phe Ala Ile Gly Arg Leu Pro Gly 
385                 390                 395                 400 


Trp Ile Ala His Tyr Arg Glu Gln Leu Gly Ala Ala Gly Asn Lys Ile 
                405                 410                 415     


Asn Arg Pro Arg Gln Val Tyr Thr Gly Asn Glu Ser Arg Lys Leu Val 
            420                 425                 430         


Pro Arg Glu Glu Arg 
        435         


<210>  9
<211>  366
<212>  PRT
<213>  Bacillus subtilis

<400>  9

Met Val His Tyr Gly Leu Lys Gly Ile Thr Cys Val Glu Thr Ser Ile 
1               5                   10                  15      


Ser His Ile Asp Gly Glu Lys Gly Arg Leu Ile Tyr Arg Gly His His 
            20                  25                  30          


Ala Lys Asp Ile Ala Leu Asn His Ser Phe Glu Glu Ala Ala Tyr Leu 
        35                  40                  45              


Ile Leu Phe Gly Lys Leu Pro Ser Thr Glu Glu Leu Gln Val Phe Lys 
    50                  55                  60                  


Asp Lys Leu Ala Ala Glu Arg Asn Leu Pro Glu His Ile Glu Arg Leu 
65                  70                  75                  80  


Ile Gln Ser Leu Pro Asn Asn Met Asp Asp Met Ser Val Leu Arg Thr 
                85                  90                  95      


Val Val Ser Ala Leu Gly Glu Asn Thr Tyr Thr Phe His Pro Lys Thr 
            100                 105                 110         


Glu Glu Ala Ile Arg Leu Ile Ala Ile Thr Pro Ser Ile Ile Ala Tyr 
        115                 120                 125             


Arg Lys Arg Trp Thr Arg Gly Glu Gln Ala Ile Ala Pro Ser Ser Gln 
    130                 135                 140                 


Tyr Gly His Val Glu Asn Tyr Tyr Tyr Met Leu Thr Gly Glu Gln Pro 
145                 150                 155                 160 


Ser Glu Ala Lys Lys Lys Ala Leu Glu Thr Tyr Met Ile Leu Ala Thr 
                165                 170                 175     


Glu His Gly Met Asn Ala Ser Thr Phe Ser Ala Arg Val Thr Leu Ser 
            180                 185                 190         


Thr Glu Ser Asp Leu Val Ser Ala Val Thr Ala Ala Leu Gly Thr Met 
        195                 200                 205             


Lys Gly Pro Leu His Gly Gly Ala Pro Ser Ala Val Thr Lys Met Leu 
    210                 215                 220                 


Glu Asp Ile Gly Glu Lys Glu His Ala Glu Ala Tyr Leu Lys Glu Lys 
225                 230                 235                 240 


Leu Glu Lys Gly Glu Arg Leu Met Gly Phe Gly His Arg Val Tyr Lys 
                245                 250                 255     


Thr Lys Asp Pro Arg Ala Glu Ala Leu Arg Gln Lys Ala Glu Glu Val 
            260                 265                 270         


Ala Gly Asn Asp Arg Asp Leu Asp Leu Ala Leu His Val Glu Ala Glu 
        275                 280                 285             


Ala Ile Arg Leu Leu Glu Ile Tyr Lys Pro Gly Arg Lys Leu Tyr Thr 
    290                 295                 300                 


Asn Val Glu Phe Tyr Ala Ala Ala Val Met Arg Ala Ile Asp Phe Asp 
305                 310                 315                 320 


Asp Glu Leu Phe Thr Pro Thr Phe Ser Ala Ser Arg Met Val Gly Trp 
                325                 330                 335     


Cys Ala His Val Leu Glu Gln Ala Glu Asn Asn Met Ile Phe Arg Pro 
            340                 345                 350         


Ser Ala Gln Tyr Thr Gly Ala Ile Pro Glu Glu Val Leu Ser 
        355                 360                 365     


<210>  10
<211>  372
<212>  PRT
<213>  Geobacillus stearothermophilus

<400>  10

Met Thr Val Thr Arg Gly Leu Glu Gly Val Val Ala Thr Thr Ser Ser 
1               5                   10                  15      


Ile Ser Ser Ile Ile Asp Asp Thr Leu Thr Tyr Val Gly Tyr Asp Ile 
            20                  25                  30          


Asp Asp Leu Ala Glu Asn Ala Ser Phe Glu Glu Val Val Tyr Leu Leu 
        35                  40                  45              


Trp His Arg Glu Leu Pro Thr Lys Glu Gln Leu Glu Glu Leu Lys Lys 
    50                  55                  60                  


Gln Leu Ala Glu Asn Ala Glu Ile Pro Asn Glu Ile Ile Glu His Phe 
65                  70                  75                  80  


Lys Leu Tyr Pro Ile Asp Lys Val His Pro Met Ala Ala Leu Arg Thr 
                85                  90                  95      


Ala Val Ser Leu Leu Gly Leu Tyr Asp Glu Glu Ala Asp Val Met Thr 
            100                 105                 110         


Lys Glu Ala Asn Tyr Arg Lys Ala Ile Arg Leu Gln Ala Lys Ile Pro 
        115                 120                 125             


Thr Ile Val Thr Ala Phe Ala Arg Val Arg Lys Gly Leu Glu Pro Val 
    130                 135                 140                 


Ala Pro Arg Lys Asp Leu Ser Phe Ala Ala Asn Phe Leu Tyr Met Leu 
145                 150                 155                 160 


Thr Gly Lys Glu Pro Asp Asp Ile Ala Thr Glu Ala Phe Asn Lys Ala 
                165                 170                 175     


Leu Val Leu His Ala Asp His Glu Leu Asn Ala Ser Thr Phe Thr Ala 
            180                 185                 190         


Arg Val Cys Val Ala Thr Leu Ser Asp Ile Tyr Ser Gly Ile Thr Ala 
        195                 200                 205             


Ala Ile Gly Ala Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Ala 
    210                 215                 220                 


Val Met Lys Met Leu Thr Glu Ile Gly Thr Val Asp Asn Val Glu Pro 
225                 230                 235                 240 


Tyr Ile Arg Arg Lys Leu Ala Asn Lys Glu Lys Ile Met Gly Phe Gly 
                245                 250                 255     


His Arg Val Tyr Arg Lys Gly Asp Pro Arg Ala Lys His Leu Lys Lys 
            260                 265                 270         


Met Ser Glu Lys Leu Thr Lys Leu Val Gly Glu Pro His Trp Tyr Glu 
        275                 280                 285             


Met Ser Thr Lys Ile Glu Glu Ile Val Thr Ser Glu Lys Ala Leu Pro 
    290                 295                 300                 


Pro Asn Val Asp Phe Tyr Ser Ala Ser Val Tyr His Cys Leu Gly Ile 
305                 310                 315                 320 


Asp His Asp Leu Phe Thr Pro Ile Phe Ala Val Ser Arg Thr Ser Gly 
                325                 330                 335     


Trp Leu Ala His Ile Leu Glu Gln Tyr Asp Asn Asn Arg Leu Ile Arg 
            340                 345                 350         


Pro Arg Ala Glu Tyr Thr Gly Pro Gly Lys Arg Ala Tyr Val Pro Ile 
        355                 360                 365             


Asp Glu Arg Gly 
    370         


<210>  11
<211>  432
<212>  PRT
<213>  Lactococcus lactis

<400>  11

Met Ile Lys Asn Ser Gln Ile Pro Ser Glu Phe Tyr Lys Lys Tyr Asn 
1               5                   10                  15      


Val Lys Lys Gly Leu Arg Asp Ile Asn Gly Lys Gly Val Leu Ala Gly 
            20                  25                  30          


Leu Thr Asn Ile Ser Ala Ile His Ser Phe Asp Lys Glu Gly Asn Gln 
        35                  40                  45              


Ile Pro Gly Ile Leu Glu Tyr Arg Ala Tyr Asn Ile Lys Asp Ile Ile 
    50                  55                  60                  


Asn Asp Leu Arg Lys Glu Asn Arg Phe Gly Phe Glu Glu Met Thr Tyr 
65                  70                  75                  80  


Leu Leu Leu Phe Gly Glu Leu Pro Thr Ala Asn Glu Leu Gln Glu Phe 
                85                  90                  95      


Gln Asn Leu Leu Ala Ser Arg Arg Thr Leu Pro Glu Phe Phe Ile Arg 
            100                 105                 110         


Glu Thr Ile Leu Thr Asn Pro Ser Ser Asp Val Met Asn Ser Met Ser 
        115                 120                 125             


Arg Cys Ile Leu Ala Leu Ala Ser Tyr Asp Glu Lys Val Ser Asp Ile 
    130                 135                 140                 


Ser Ile Glu Asn Val Leu Glu Gln Ser Phe Gly Leu Ile Ala Asp Phe 
145                 150                 155                 160 


Pro Leu Leu Ala Ile Tyr Ser Tyr Gln Ser Tyr Val His Tyr Phe Lys 
                165                 170                 175     


Lys Glu Ser Leu Tyr Ile His Tyr Pro Asp Pro Lys Met Thr Thr Ala 
            180                 185                 190         


Glu Asn Ile Leu Arg Met Leu Arg Pro Asp Cys His Tyr Thr Glu Val 
        195                 200                 205             


Glu Ala Lys Val Leu Asp Ile Ala Leu Ile Leu His Met Glu His Gly 
    210                 215                 220                 


Gly Gly Asn Asn Ser Thr Phe Thr Thr His Val Val Thr Ser Ser Gly 
225                 230                 235                 240 


Thr Asp Thr Tyr Ala Thr Ile Ala Ala Ala Leu Ser Ser Leu Lys Gly 
                245                 250                 255     


Pro Lys His Gly Gly Ala Asn Ile Lys Ala Ala Lys Met Leu Glu Asn 
            260                 265                 270         


Ile Lys Glu Asn Ile Ser Asn Tyr Glu Asp Asp Ala Glu Ile Glu Lys 
        275                 280                 285             


Tyr Leu His Lys Ile Leu Asn Lys Glu Val Phe Asp Lys Gln Gly Leu 
    290                 295                 300                 


Ile Tyr Gly Ile Gly His Ala Ile Tyr Ser Leu Ser Asp Pro Arg Phe 
305                 310                 315                 320 


Glu Val Phe Lys Ser Tyr Val Glu Thr Leu Val Lys Glu Lys Gly Leu 
                325                 330                 335     


Glu Glu Glu Phe Lys Leu Tyr Glu Lys Val Ala Arg Leu Ala Pro Lys 
            340                 345                 350         


Val Ile Ser Glu Asn Arg Lys Ile Tyr Lys Thr Ile Cys Pro Asn Val 
        355                 360                 365             


Asp Phe Tyr Ser Gly Phe Val Tyr Arg Ile Leu Glu Ile Pro Gln Glu 
    370                 375                 380                 


Leu Phe Thr Pro Leu Phe Ala Ile Ala Arg Ile Val Gly Trp Leu Ala 
385                 390                 395                 400 


His Arg Ile Glu Glu Leu Ile Asn Met Asn Lys Ile Ile Arg Pro Ala 
                405                 410                 415     


Tyr Glu Ser Val Leu Glu Ser Lys Asn Tyr Ile Lys Leu Gly Glu Arg 
            420                 425                 430         


<210>  12
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  12
gggaaaggta ccatgatggt gcgtatcttt gacacgac                               38


<210>  13
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  13
gggaaactca gatcacacca gtttgcccgt cac                                    33


<210>  14
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  14
tcatgcaaaa cactgcttcc agatg                                             25


<210>  15
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  15
agagtagaat tcgacgttcg ggtacag                                           27


<210>  16
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  16
tgtacccgaa cgtcgaattc tactctg                                           27


<210>  17
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  17
cgtgccattg caataatgac ggtgaac                                           27


<210>  18
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  18
gttcaccgtc attattgcaa tggcacg                                           27


<210>  19
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  19
gccattgcca gaatgacggt gaacatg                                           27


<210>  20
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  20
ccgtcattct ggcaatggca cgtacc                                            26


<210>  21
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  21
gtgccattgc cataatgacg gtgaacatg                                         29


<210>  22
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  22
cgtcattatg gcaatggcac gtac                                              24


<210>  23
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  23
gtgccattgc cacaatgacg gtgaac                                            26


<210>  24
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  24
cgtcattgtg gcaatggcac gtac                                              24


<210>  25
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  25
gaagcagctc cagcctacac caacttagca atcaaccatt aacgc                       45


<210>  26
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  26
gcgttaatgg ttgattgcta agttggtgta ggctggagct gcttc                       45


<210>  27
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  27
catatgaacg gcgggttaaa atatttaatg ggaattagcc atggtccata tg               52


<210>  28
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  28
catatggacc atggctaatt cccattaaat attttaaccc gccgttcata tg               52


<210>  29
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  29
gttgtcgtga cttgtccaag atctatg                                           27


<210>  30
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  30
actacgggca cagaggttaa ctttc                                             25


<210>  31
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  31
ctgcctcgtc ctgcagttca ttc                                               23


