                         SEQUENCE LISTING

<110>  Genia Technologies, Inc.
 
<120>  SITE-SPECIFIC BIO-CONJUGATION METHODS AND COMPOSITIONS USEFUL FOR
        NANOPORE SYSTEMS

<130>  P33506-WO

<150>  US 62/313,086
<151>  2016-03-24

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  1

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  2
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  2

Ala His Ile Val Met Val Asp Ala Tyr Lys 
1               5                   10  


<210>  3
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide

<400>  3

Ala His Ile Val Met Val Asp Ala 
1               5               


<210>  4
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  X is 2-aminobutyric acid

<400>  4

Xaa Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10                  


<210>  5
<211>  143
<212>  PRT
<213>  Artificial sequence

<220>
<223>  143 aa fragment of CnaB2 domain of the Streptococcus pycogenes 
       fibronectin binding protein, FbaB

<400>  5

Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr Thr 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser Gly Leu 
            20                  25                  30          


Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu Asp Ser 
        35                  40                  45              


Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu 
    50                  55                  60                  


Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser 
65                  70                  75                  80  


Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly 
                85                  90                  95      


Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala 
            100                 105                 110         


Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn 
        115                 120                 125             


Gly Lys Ala Thr Lys Gly Asp Ala His Ile Val Met Val Asp Ala 
    130                 135                 140             


<210>  6
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  129 aa N-terminal CnaB2 domain sequence fragment of the 
       Streptococcus pycogenes fibronectin binding protein, FbaB

<400>  6

Asp Tyr Asp Ile Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met 
1               5                   10                  15      


Val Asp Thr Leu Ser Gly Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp 
            20                  25                  30          


Met Thr Ile Glu Glu Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg 
        35                  40                  45              


Asp Glu Asp Gly Lys Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp 
    50                  55                  60                  


Ser Ser Gly Lys Thr Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys 
65                  70                  75                  80  


Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala 
                85                  90                  95      


Pro Asp Gly Tyr Glu Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu 
            100                 105                 110         


Gln Gly Gln Val Thr Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His 
        115                 120                 125             


Ile 
    


<210>  7
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  138 aa N-terminal CnaB2 domain sequence fragment of the 
       Streptococcus pycogenes fibronectin binding protein, FbaB

<400>  7

Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr Thr 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser Gly Leu 
            20                  25                  30          


Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu Asp Ser 
        35                  40                  45              


Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu 
    50                  55                  60                  


Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser 
65                  70                  75                  80  


Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly 
                85                  90                  95      


Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala 
            100                 105                 110         


Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn 
        115                 120                 125             


Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
    130                 135             


<210>  8
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion of fragment of CnaB2 domain of the Streptococcus pycogenes
       fibronectin binding protein, FbaB with N-terminal His tag and 
       C-terminal GGS linker

<400>  8

Met His His His His His His His His Ser Gly Asp Tyr Asp Ile Pro 
1               5                   10                  15      


Thr Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser 
            20                  25                  30          


Gly Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu 
        35                  40                  45              


Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
    50                  55                  60                  


Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
65                  70                  75                  80  


Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
                85                  90                  95      


Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
            100                 105                 110         


Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
        115                 120                 125             


Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile Gly Gly Ser 
    130                 135                 140             


<210>  9
<211>  726
<212>  PRT
<213>  Clostridium phage phiCPV4

<400>  9

Asp Lys His Thr Gln Tyr Val Lys Glu His Ser Phe Asn Tyr Asp Glu 
1               5                   10                  15      


Tyr Lys Lys Ala Asn Phe Asp Lys Ile Glu Cys Leu Ile Phe Asp Thr 
            20                  25                  30          


Glu Ser Cys Thr Asn Tyr Glu Asn Asp Asn Thr Gly Ala Arg Val Tyr 
        35                  40                  45              


Gly Trp Gly Leu Gly Val Thr Arg Asn His Asn Met Ile Tyr Gly Gln 
    50                  55                  60                  


Asn Leu Asn Gln Phe Trp Glu Val Cys Gln Asn Ile Phe Asn Asp Trp 
65                  70                  75                  80  


Tyr His Asp Asn Lys His Thr Ile Lys Ile Thr Lys Thr Lys Lys Gly 
                85                  90                  95      


Phe Pro Lys Arg Lys Tyr Ile Lys Phe Pro Ile Ala Val His Asn Leu 
            100                 105                 110         


Gly Trp Asp Val Glu Phe Leu Lys Tyr Ser Leu Val Glu Asn Gly Phe 
        115                 120                 125             


Asn Tyr Asp Lys Gly Leu Leu Lys Thr Val Phe Ser Lys Gly Ala Pro 
    130                 135                 140                 


Tyr Gln Thr Val Thr Asp Val Glu Glu Pro Lys Thr Phe His Ile Val 
145                 150                 155                 160 


Gln Asn Asn Asn Ile Val Tyr Gly Cys Asn Val Tyr Met Asp Lys Phe 
                165                 170                 175     


Phe Glu Val Glu Asn Lys Asp Gly Ser Thr Thr Glu Ile Gly Leu Cys 
            180                 185                 190         


Leu Asp Phe Phe Asp Ser Tyr Lys Ile Ile Thr Cys Ala Glu Ser Gln 
        195                 200                 205             


Phe His Asn Tyr Val His Asp Val Asp Pro Met Phe Tyr Lys Met Gly 
    210                 215                 220                 


Glu Glu Tyr Asp Tyr Asp Thr Trp Arg Ser Pro Thr His Lys Gln Thr 
225                 230                 235                 240 


Thr Leu Glu Leu Arg Tyr Gln Tyr Asn Asp Ile Tyr Met Leu Arg Glu 
                245                 250                 255     


Val Ile Glu Gln Phe Tyr Ile Asp Gly Leu Cys Gly Gly Glu Leu Pro 
            260                 265                 270         


Leu Thr Gly Met Arg Thr Ala Ser Ser Ile Ala Phe Asn Val Leu Lys 
        275                 280                 285             


Lys Met Thr Phe Gly Glu Glu Lys Thr Glu Glu Gly Tyr Ile Asn Tyr 
    290                 295                 300                 


Phe Glu Leu Asp Lys Lys Thr Lys Phe Glu Phe Leu Arg Lys Arg Ile 
305                 310                 315                 320 


Glu Met Glu Ser Tyr Thr Gly Gly Tyr Thr His Ala Asn His Lys Ala 
                325                 330                 335     


Val Gly Lys Thr Ile Asn Lys Ile Gly Cys Ser Leu Asp Ile Asn Ser 
            340                 345                 350         


Ser Tyr Pro Ser Gln Met Ala Tyr Lys Val Phe Pro Tyr Gly Lys Pro 
        355                 360                 365             


Val Arg Lys Thr Trp Gly Arg Lys Pro Lys Thr Glu Lys Asn Glu Val 
    370                 375                 380                 


Tyr Leu Ile Glu Val Gly Phe Asp Phe Val Glu Pro Lys His Glu Glu 
385                 390                 395                 400 


Tyr Ala Leu Asp Ile Phe Lys Ile Gly Ala Val Asn Ser Lys Ala Leu 
                405                 410                 415     


Ser Pro Ile Thr Gly Ala Val Ser Gly Gln Glu Tyr Phe Cys Thr Asn 
            420                 425                 430         


Ile Lys Asp Gly Lys Ala Ile Pro Val Tyr Lys Glu Leu Lys Asp Thr 
        435                 440                 445             


Lys Leu Thr Thr Asn Tyr Asn Val Val Leu Thr Ser Val Glu Tyr Glu 
    450                 455                 460                 


Phe Trp Ile Lys His Phe Asn Phe Gly Val Phe Lys Lys Asp Glu Tyr 
465                 470                 475                 480 


Asp Cys Phe Glu Val Asp Asn Leu Glu Phe Thr Gly Leu Lys Ile Gly 
                485                 490                 495     


Ser Ile Leu Tyr Tyr Lys Ala Glu Lys Gly Lys Phe Lys Pro Tyr Val 
            500                 505                 510         


Asp His Phe Thr Lys Met Lys Val Glu Asn Lys Lys Leu Gly Asn Lys 
        515                 520                 525             


Pro Leu Thr Asn Gln Ala Lys Leu Ile Leu Asn Gly Ala Tyr Gly Lys 
    530                 535                 540                 


Phe Gly Thr Lys Gln Asn Lys Glu Glu Lys Asp Leu Ile Met Asp Lys 
545                 550                 555                 560 


Asn Gly Leu Leu Thr Phe Thr Gly Ser Val Thr Glu Tyr Glu Gly Lys 
                565                 570                 575     


Glu Phe Tyr Arg Pro Tyr Ala Ser Phe Val Thr Ala Tyr Gly Arg Leu 
            580                 585                 590         


Gln Leu Trp Asn Ala Ile Ile Tyr Ala Val Gly Val Glu Asn Phe Leu 
        595                 600                 605             


Tyr Cys Asp Thr Asp Ser Ile Tyr Cys Asn Arg Glu Val Asn Ser Leu 
    610                 615                 620                 


Ile Glu Asp Met Asn Ala Ile Gly Glu Thr Ile Asp Lys Thr Ile Leu 
625                 630                 635                 640 


Gly Lys Trp Asp Val Glu His Val Phe Asp Lys Phe Lys Val Leu Gly 
                645                 650                 655     


Gln Lys Lys Tyr Met Tyr His Asp Cys Lys Glu Asp Lys Thr Asp Leu 
            660                 665                 670         


Lys Cys Cys Gly Leu Pro Ser Asp Ala Arg Lys Ile Ile Ile Gly Gln 
        675                 680                 685             


Gly Phe Asp Glu Phe Tyr Leu Gly Lys Asn Val Glu Gly Lys Lys Gln 
    690                 695                 700                 


Arg Lys Lys Val Ile Gly Gly Cys Leu Leu Leu Asp Thr Leu Phe Thr 
705                 710                 715                 720 


Ile Lys Lys Ile Met Phe 
                725     


<210>  10
<211>  869
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic fusion protein (Pol6-SpyCatcher)

<400>  10

Met His His His His His His His His Ser Gly Asp Tyr Asp Ile Pro 
1               5                   10                  15      


Thr Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser 
            20                  25                  30          


Gly Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu 
        35                  40                  45              


Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
    50                  55                  60                  


Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
65                  70                  75                  80  


Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
                85                  90                  95      


Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
            100                 105                 110         


Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
        115                 120                 125             


Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile Gly Gly Ser Asp 
    130                 135                 140                 


Lys His Thr Gln Tyr Val Lys Glu His Ser Phe Asn Tyr Asp Glu Tyr 
145                 150                 155                 160 


Lys Lys Ala Asn Phe Asp Lys Ile Glu Cys Leu Ile Phe Asp Thr Glu 
                165                 170                 175     


Ser Cys Thr Asn Tyr Glu Asn Asp Asn Thr Gly Ala Arg Val Tyr Gly 
            180                 185                 190         


Trp Gly Leu Gly Val Thr Arg Asn His Asn Met Ile Tyr Gly Gln Asn 
        195                 200                 205             


Leu Asn Gln Phe Trp Glu Val Cys Gln Asn Ile Phe Asn Asp Trp Tyr 
    210                 215                 220                 


His Asp Asn Lys His Thr Ile Lys Ile Thr Lys Thr Lys Lys Gly Phe 
225                 230                 235                 240 


Pro Lys Arg Lys Tyr Ile Lys Phe Pro Ile Ala Val His Asn Leu Gly 
                245                 250                 255     


Trp Asp Val Glu Phe Leu Lys Tyr Ser Leu Val Glu Asn Gly Phe Asn 
            260                 265                 270         


Tyr Asp Lys Gly Leu Leu Lys Thr Val Phe Ser Lys Gly Ala Pro Tyr 
        275                 280                 285             


Gln Thr Val Thr Asp Val Glu Glu Pro Lys Thr Phe His Ile Val Gln 
    290                 295                 300                 


Asn Asn Asn Ile Val Tyr Gly Cys Asn Val Tyr Met Asp Lys Phe Phe 
305                 310                 315                 320 


Glu Val Glu Asn Lys Asp Gly Ser Thr Thr Glu Ile Gly Leu Cys Leu 
                325                 330                 335     


Asp Phe Phe Asp Ser Tyr Lys Ile Ile Thr Cys Ala Glu Ser Gln Phe 
            340                 345                 350         


His Asn Tyr Val His Asp Val Asp Pro Met Phe Tyr Lys Met Gly Glu 
        355                 360                 365             


Glu Tyr Asp Tyr Asp Thr Trp Arg Ser Pro Thr His Lys Gln Thr Thr 
    370                 375                 380                 


Leu Glu Leu Arg Tyr Gln Tyr Asn Asp Ile Tyr Met Leu Arg Glu Val 
385                 390                 395                 400 


Ile Glu Gln Phe Tyr Ile Asp Gly Leu Cys Gly Gly Glu Leu Pro Leu 
                405                 410                 415     


Thr Gly Met Arg Thr Ala Ser Ser Ile Ala Phe Asn Val Leu Lys Lys 
            420                 425                 430         


Met Thr Phe Gly Glu Glu Lys Thr Glu Glu Gly Tyr Ile Asn Tyr Phe 
        435                 440                 445             


Glu Leu Asp Lys Lys Thr Lys Phe Glu Phe Leu Arg Lys Arg Ile Glu 
    450                 455                 460                 


Met Glu Ser Tyr Thr Gly Gly Tyr Thr His Ala Asn His Lys Ala Val 
465                 470                 475                 480 


Gly Lys Thr Ile Asn Lys Ile Gly Cys Ser Leu Asp Ile Asn Ser Ser 
                485                 490                 495     


Tyr Pro Ser Gln Met Ala Tyr Lys Val Phe Pro Tyr Gly Lys Pro Val 
            500                 505                 510         


Arg Lys Thr Trp Gly Arg Lys Pro Lys Thr Glu Lys Asn Glu Val Tyr 
        515                 520                 525             


Leu Ile Glu Val Gly Phe Asp Phe Val Glu Pro Lys His Glu Glu Tyr 
    530                 535                 540                 


Ala Leu Asp Ile Phe Lys Ile Gly Ala Val Asn Ser Lys Ala Leu Ser 
545                 550                 555                 560 


Pro Ile Thr Gly Ala Val Ser Gly Gln Glu Tyr Phe Cys Thr Asn Ile 
                565                 570                 575     


Lys Asp Gly Lys Ala Ile Pro Val Tyr Lys Glu Leu Lys Asp Thr Lys 
            580                 585                 590         


Leu Thr Thr Asn Tyr Asn Val Val Leu Thr Ser Val Glu Tyr Glu Phe 
        595                 600                 605             


Trp Ile Lys His Phe Asn Phe Gly Val Phe Lys Lys Asp Glu Tyr Asp 
    610                 615                 620                 


Cys Phe Glu Val Asp Asn Leu Glu Phe Thr Gly Leu Lys Ile Gly Ser 
625                 630                 635                 640 


Ile Leu Tyr Tyr Lys Ala Glu Lys Gly Lys Phe Lys Pro Tyr Val Asp 
                645                 650                 655     


His Phe Thr Lys Met Lys Val Glu Asn Lys Lys Leu Gly Asn Lys Pro 
            660                 665                 670         


Leu Thr Asn Gln Ala Lys Leu Ile Leu Asn Gly Ala Tyr Gly Lys Phe 
        675                 680                 685             


Gly Thr Lys Gln Asn Lys Glu Glu Lys Asp Leu Ile Met Asp Lys Asn 
    690                 695                 700                 


Gly Leu Leu Thr Phe Thr Gly Ser Val Thr Glu Tyr Glu Gly Lys Glu 
705                 710                 715                 720 


Phe Tyr Arg Pro Tyr Ala Ser Phe Val Thr Ala Tyr Gly Arg Leu Gln 
                725                 730                 735     


Leu Trp Asn Ala Ile Ile Tyr Ala Val Gly Val Glu Asn Phe Leu Tyr 
            740                 745                 750         


Cys Asp Thr Asp Ser Ile Tyr Cys Asn Arg Glu Val Asn Ser Leu Ile 
        755                 760                 765             


Glu Asp Met Asn Ala Ile Gly Glu Thr Ile Asp Lys Thr Ile Leu Gly 
    770                 775                 780                 


Lys Trp Asp Val Glu His Val Phe Asp Lys Phe Lys Val Leu Gly Gln 
785                 790                 795                 800 


Lys Lys Tyr Met Tyr His Asp Cys Lys Glu Asp Lys Thr Asp Leu Lys 
                805                 810                 815     


Cys Cys Gly Leu Pro Ser Asp Ala Arg Lys Ile Ile Ile Gly Gln Gly 
            820                 825                 830         


Phe Asp Glu Phe Tyr Leu Gly Lys Asn Val Glu Gly Lys Lys Gln Arg 
        835                 840                 845             


Lys Lys Val Ile Gly Gly Cys Leu Leu Leu Asp Thr Leu Phe Thr Ile 
    850                 855                 860                 


Lys Lys Ile Met Phe 
865                 


<210>  11
<211>  2610
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic nucleotide sequence encoding Pol6-SpyCatcher fusion 
       protein

<400>  11
atgcatcacc atcatcatca ccaccacagc ggtgactacg acatcccgac caccgagaac       60

ctgtacttcc agggcgccat ggtggacaca ctgagcggtc tgagcagtga acagggccag      120

agcggcgaca tgaccattga agaggacagc gccacccaca tcaagttcag caagcgtgac      180

gaggacggta aggaactggc cggcgccacc atggaactgc gtgacagcag cggcaagacc      240

atcagcacct ggatcagcga tggccaggtg aaggacttct acctgtaccc gggcaagtac      300

accttcgtgg agacagccgc accggacggt tacgaggttg ccaccgccat caccttcacc      360

gtgaacgagc agggccaagt gaccgttaac ggcaaggcca ccaagggtga cgcccacatc      420

ggcggttccg acaaacacac gcagtacgtc aaagagcata gcttcaatta tgacgagtat      480

aagaaagcga atttcgacaa gatcgagtgc ctgatctttg acaccgagag ctgcacgaat      540

tatgagaacg ataataccgg tgcacgtgtt tacggttggg gtcttggcgt cacccgcaac      600

cacaatatga tctacggcca aaatctgaat cagttttggg aagtatgcca gaacattttc      660

aatgattggt atcacgacaa caaacatacc attaagatta ccaagaccaa gaaaggcttc      720

ccgaaacgta agtacattaa gtttccgatt gcagttcaca atttgggctg ggatgttgaa      780

ttcctgaagt atagcctggt ggagaatggt ttcaattacg acaagggtct gctgaaaact      840

gtttttagca agggtgcgcc gtaccaaacc gtgaccgatg ttgaggaacc gaaaacgttc      900

catatcgtcc agaataacaa catcgtttat ggttgtaacg tgtatatgga caaattcttt      960

gaggtcgaga acaaagacgg ctctaccacc gagattggcc tgtgcttgga tttcttcgat     1020

agctataaga tcatcacgtg tgctgagagc cagttccaca attacgttca tgatgtggat     1080

ccaatgttct acaaaatggg tgaagagtat gattacgata cttggcgtag cccgacgcac     1140

aagcagacca ccctggagct gcgctaccaa tacaatgata tctatatgct gcgtgaagtc     1200

atcgaacagt tttacattga cggtttatgt ggcggcgagc tgccgctgac cggcatgcgc     1260

accgcttcca gcattgcgtt caacgtgctg aaaaagatga cctttggtga ggaaaagacg     1320

gaagagggct acatcaacta ttttgaattg gacaagaaaa ccaaattcga gtttctgcgt     1380

aagcgcattg aaatggaatc gtacaccggt ggctatacgc acgcaaatca caaagccgtt     1440

ggtaagacta ttaacaagat cggttgctct ttggacatta acagctcata cccttcgcag     1500

atggcgtaca aggtctttcc gtatggcaaa ccggttcgta agacctgggg tcgtaaacca     1560

aagaccgaga agaacgaagt ttatctgatt gaagttggct ttgacttcgt ggagccgaaa     1620

cacgaagaat acgcgctgga tatctttaag attggtgcgg tgaactctaa agcgctgagc     1680

ccgatcaccg gcgctgtcag cggtcaagag tatttctgta cgaacattaa agacggcaaa     1740

gcaatcccgg tttacaaaga actgaaggac accaaattga ccactaacta caatgtcgtg     1800

ctgaccagcg tggagtacga gttctggatc aaacacttca attttggtgt gtttaagaaa     1860

gacgagtacg actgtttcga agttgacaat ctggagttta cgggtctgaa gattggttcc     1920

attctgtact acaaggcaga gaaaggcaag tttaaacctt acgtggatca cttcacgaaa     1980

atgaaagtgg agaacaagaa actgggtaat aagccgctga cgaatcaggc aaagctgatt     2040

ctgaacggtg cgtacggcaa attcggcacc aaacaaaaca aagaagagaa agatttgatc     2100

atggataaga acggtttgct gaccttcacg ggtagcgtca cggaatacga gggtaaagaa     2160

ttctatcgtc cgtatgcgag cttcgttact gcctatggtc gcctgcaact gtggaacgcg     2220

attatctacg cggttggtgt ggagaatttt ctgtactgcg acaccgacag catctattgt     2280

aaccgtgaag ttaacagcct cattgaggat atgaacgcca ttggtgaaac catcgataaa     2340

acgattctgg gtaaatggga cgtggagcat gtctttgata agtttaaggt cctgggccag     2400

aagaagtaca tgtatcatga ttgcaaagaa gataaaacgg acctgaagtg ttgcggtctg     2460

ccgagcgatg cccgtaagat tatcattggt caaggtttcg acgagtttta tctgggcaaa     2520

aatgtcgaag gtaagaagca acgcaaaaaa gtgatcggcg gttgcctgct gctggacacc     2580

ctgtttacga tcaagaaaat catgttctaa                                      2610


<210>  12
<211>  99
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Self-priming DNA template sequence

<400>  12
tttttgcgct cgagatctcc gtaaggagat ctcgagcgcg ggactactac tgggatcatc       60

atagccacct cagctgcacg taagtgcagc tgaggtggc                              99


<210>  13
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic oligonucleotide tag


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-cyanine3-phosphate-dT

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  3'-propanol-dT

<400>  13
ttttntntnt nttttttttt tttttttttt                                        30


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide tag


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-cyanine3-phosphate-dT

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  3'-propanol-dT

<400>  14
tttttttttt tttttttttt tttttttttt                                        30


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide tag


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-cyanine3-phosphate-dT

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  N3-cyanoethyl-dT

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  3'-propanol-dT

<400>  15
ttttnnnttt tttttttttt tttttttttt                                        30


<210>  16
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide tag


<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  thymidine methylphosphonate

<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  3'-propanol-dT

<400>  16
ttttttnnnn nntttttttt tttttttttt t                                      31


