                         SEQUENCE LISTING

<110>  UNIVERSITETET I TROMS - NORGES ARKTISKE UNIVERSITET
 
<120>  Marine DNA Polymerases

<130>  59.15.123840/02

<150>  GB1604876.1
<151>  2016-03-22

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated PB polymerase

<400>  1

Thr Glu Val Ala Phe Glu Ile Val Glu Glu Ile Asp Ser Thr Ile Leu 
1               5                   10                  15      


Asp Lys Val Met Ser Val His Leu Glu Met Tyr Asp Gly Gln Tyr His 
            20                  25                  30          


Thr Ser Glu Leu Leu Gly Ile Ala Leu Ser Asp Gly Glu Lys Gly Tyr 
        35                  40                  45              


Phe Ala Pro Ala Asp Ile Ala Phe Gln Ser Lys Asp Phe Cys Ser Trp 
    50                  55                  60                  


Leu Glu Asn Ala Thr Asn Lys Lys Tyr Leu Ala Asp Ser Lys Ala Thr 
65                  70                  75                  80  


Gln Ala Val Ser Arg Lys His Asn Val Asn Val His Gly Val Glu Phe 
                85                  90                  95      


Asp Leu Leu Leu Ala Ala Tyr Ile Val Asn Pro Ala Ile Ser Ser Glu 
            100                 105                 110         


Asp Val Ala Ala Ile Ala Lys Glu Phe Gly Tyr Phe Asn Leu Leu Thr 
        115                 120                 125             


Asn Asp Ser Val Tyr Gly Lys Gly Ala Lys Lys Thr Ala Pro Glu Ile 
    130                 135                 140                 


Glu Lys Ile Ala Glu His Ala Val Arg Lys Ala Arg Ala Ile Trp Asp 
145                 150                 155                 160 


Leu Lys Glu Lys Leu Glu Val Lys Leu Glu Glu Asn Glu Gln Tyr Ala 
                165                 170                 175     


Leu Tyr Lys Glu Ile Glu Leu Pro Leu Ala Ser Ile Leu Gly Thr Met 
            180                 185                 190         


Glu Ser Asp Gly Val Leu Val Asp Lys Gln Ile Leu Val Glu Met Gly 
        195                 200                 205             


His Glu Leu Asn Ile Lys Leu Arg Ala Ile Glu Gln Asp Ile Tyr Ala 
    210                 215                 220                 


Leu Ala Gly Glu Thr Phe Asn Ile Asn Ser Pro Lys Gln Leu Gly Val 
225                 230                 235                 240 


Ile Leu Phe Glu Lys Ile Gly Leu Thr Pro Ile Lys Lys Thr Lys Thr 
                245                 250                 255     


Gly Tyr Ser Thr Ala Ala Asp Val Leu Glu Lys Leu Ala Ser Glu His 
            260                 265                 270         


Glu Ile Ile Glu Gln Ile Leu Leu Tyr Arg Gln Leu Gly Lys Leu Asn 
        275                 280                 285             


Ser Thr Tyr Ile Glu Gly Leu Leu Lys Glu Ile His Glu Asp Asp Gly 
    290                 295                 300                 


Lys Ile His Thr Arg Tyr Gln Gln Ala Leu Thr Ser Thr Gly Arg Leu 
305                 310                 315                 320 


Ser Ser Ile Asn Pro Asn Leu Gln Asn Ile Pro Val Arg Leu Glu Glu 
                325                 330                 335     


Gly Arg Lys Ile Arg Lys Ala Phe Val Pro Ser Gln Pro Gly Trp Val 
            340                 345                 350         


Met Phe Ala Ala Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His 
        355                 360                 365             


Met Ser Glu Asp Glu Asn Leu Val Glu Ala Phe Asn Asn Asp Leu Asp 
    370                 375                 380                 


Ile His Thr Lys Thr Ala Met Asp Val Phe His Val Glu Gln Glu Ala 
385                 390                 395                 400 


Val Thr Ser Asp Met Arg Arg Ala Ala Lys Ala Val Asn Phe Gly Ile 
                405                 410                 415     


Val Tyr Gly Ile Ser Asp Tyr Gly Leu Ser Gln Asn Leu Asp Ile Thr 
            420                 425                 430         


Arg Lys Glu Ala Ala Thr Phe Ile Glu Asn Tyr Leu Asn Ser Phe Pro 
        435                 440                 445             


Gly Val Lys Gly Tyr Met Asp Asp Ile Val Gln Asp Ala Lys Gln Thr 
    450                 455                 460                 


Gly Tyr Val Thr Thr Ile Leu Asn Arg Arg Arg Tyr Leu Pro Glu Ile 
465                 470                 475                 480 


Thr Ser Ser Asn Phe Asn Leu Arg Ser Phe Ala Glu Arg Thr Ala Met 
                485                 490                 495     


Asn Thr Pro Ile Gln Gly Ser Ala Ala Asp Ile Ile Lys Lys Ala Met 
            500                 505                 510         


Ile Asp Met Ala Glu Arg Leu Ile Ser Glu Asn Met Gln Thr Lys Met 
        515                 520                 525             


Leu Leu Gln Val His Asp Glu Leu Ile Phe Glu Ala Pro Pro Glu Glu 
    530                 535                 540                 


Ile Ala Met Leu Glu Lys Ile Val Pro Glu Val Met Glu Asn Ala Ile 
545                 550                 555                 560 


Lys Leu Ile Val Pro Leu Lys Val Asp Tyr Ala Phe Gly Ser Ser Trp 
                565                 570                 575     


Tyr Asp Thr Lys 
            580 


<210>  2
<211>  1743
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Truncated PB polymerase

<400>  2
acagaagtag cattcgagat tgttgaagaa attgactcta caatattaga taaagtaatg       60

tcagtccatt tagaaatgta tgatgggcaa tatcatacaa gcgaattatt aggtattgct      120

ttatcagatg gagaaaaggg ttattttgct cctgctgata tagcttttca atcgaaggat      180

ttttgttctt ggttagaaaa tgctacgaat aaaaagtatt tagcagactc caaagcaaca      240

caagcagtga gtagaaaaca taatgtgaat gtacatggag tggaattcga ccttctttta      300

gcagcgtata tagtaaatcc tgctatctct tcagaggatg ttgctgctat tgctaaagaa      360

tttggatatt ttaacttgct gacaaacgat agtgtttatg ggaaaggtgc caaaaaaacc      420

gcacctgaaa tcgagaaaat tgcagaacat gccgtaagaa aagcaagggc tatttgggac      480

ttgaaagaaa agttagaagt aaaactggaa gaaaatgaac aatatgcgtt gtataaagaa      540

atagagctac cgcttgcatc tatccttggt acgatggaat cagatggggt gctggtggat      600

aaacaaattc ttgtagaaat gggtcatgag cttaatatta agttacgagc gattgaacaa      660

gacatttatg cgttagctgg tgaaacgttt aatattaatt cacctaaaca attaggtgta      720

atactatttg aaaaaattgg tcttacccct attaaaaaga caaaaacggg ctattcaact      780

gcagcagatg ttttggaaaa actagcaagt gaacatgaaa taatagagca aattttacta      840

tatcgtcaat taggtaaact caattccaca tatatcgaag gattattaaa agagattcat      900

gaagatgatg ggaagatcca tacccgatat caacaagccc taacttcaac tgggcgtttg      960

agttcgatca atccaaacct tcaaaatata ccagttcgtt tagaagaagg tagaaaaata     1020

cgtaaagcct ttgttccttc acaaccggga tgggtaatgt ttgcggcgga ttactctcaa     1080

attgaattgc gtgttcttgc ccatatgtct gaggatgaaa acctggtaga agcttttaat     1140

aatgatctgg atattcatac taaaacggct atggatgtat tccatgtgga gcaggaagca     1200

gtaacgtccg atatgcgccg tgctgctaag gcagttaact ttgggattgt gtatggtatt     1260

agtgattatg gtttatcaca aaacctagat attactagaa aagaagcggc gacatttatc     1320

gagaattatt taaatagctt cccaggtgta aaaggatata tggatgatat cgttcaagat     1380

gcgaaacaaa caggctacgt tacaacaatt ttgaatagac gaagatattt gcctgaaata     1440

acaagttcta actttaatct ccgcagtttt gcagaacgta ctgctatgaa tacaccaatt     1500

caagggagtg cagccgatat tattaaaaaa gcaatgatcg atatggcgga aagattaata     1560

tcagaaaata tgcagaccaa aatgctacta caagtacatg atgaattaat ttttgaggct     1620

ccaccagagg aaattgcaat gctagaaaaa atagtgccag aggtgatgga aaacgctatt     1680

aaactgattg tacctttgaa agtggattat gcctttggtt catcttggta tgacacgaag     1740

tag                                                                   1743


<210>  3
<211>  877
<212>  PRT
<213>  Psychrobacillus sp.

<400>  3

Met Tyr Leu Ser Thr Glu Lys Ile Leu Leu Leu Asp Gly Asn Ser Leu 
1               5                   10                  15      


Ala Tyr Arg Ala Phe Phe Ala Leu Pro Leu Leu Thr Asn Glu His Gly 
            20                  25                  30          


Ile His Thr Asn Ala Val Tyr Gly Phe Thr Met Met Leu Gln Lys Ile 
        35                  40                  45              


Met Asp Glu Glu Asn Pro Thr His Met Leu Val Ala Phe Asp Ala Gly 
    50                  55                  60                  


Lys Thr Thr Phe Arg His Ser Thr Phe Gly Asp Tyr Lys Gly Gly Arg 
65                  70                  75                  80  


Gln Lys Thr Pro Pro Glu Leu Ser Glu Gln Phe Pro Tyr Ile Arg Lys 
                85                  90                  95      


Leu Ile Asp Ala Tyr Gly Ile Lys Arg Tyr Glu Leu Glu Met Tyr Glu 
            100                 105                 110         


Ala Asp Asp Ile Ile Gly Thr Leu Ser Lys Arg Ala Asp Glu Lys Gly 
        115                 120                 125             


Gln Gln Val Val Ile Val Ser Gly Asp Lys Asp Leu Thr Gln Leu Ala 
    130                 135                 140                 


Thr Asp Lys Thr Thr Val Tyr Ile Thr Arg Lys Gly Ile Thr Asp Ile 
145                 150                 155                 160 


Glu Lys Tyr Thr Pro Glu His Val Gln Glu Lys Tyr Gly Leu Thr Pro 
                165                 170                 175     


Leu Gln Ile Ile Asp Met Lys Gly Leu Met Gly Asp Ala Ser Asp Asn 
            180                 185                 190         


Ile Pro Gly Val Pro Gly Val Gly Glu Lys Thr Ala Ile Lys Leu Leu 
        195                 200                 205             


Lys Glu His Gly Ser Val Glu Asp Leu Tyr Lys Ala Leu Asp Thr Val 
    210                 215                 220                 


Ser Gly Val Lys Leu Lys Glu Lys Leu Ile Ala Asn Glu Glu Gln Ala 
225                 230                 235                 240 


Ile Met Ser Lys Ala Leu Ala Thr Ile Glu Thr Ala Ala Pro Ile Gln 
                245                 250                 255     


Ile Ser Ile Asp Asp Leu Ser Tyr Thr Gly Pro Asn Met Glu Glu Val 
            260                 265                 270         


Ile Glu Val Trp Lys Glu Leu Ala Phe Lys Thr Leu Leu Glu Lys Ser 
        275                 280                 285             


Asp Tyr Ile Ser Glu Glu Ser Glu Thr Thr Glu Val Ala Phe Glu Ile 
    290                 295                 300                 


Val Glu Glu Ile Asp Ser Thr Ile Leu Asp Lys Val Met Ser Val His 
305                 310                 315                 320 


Leu Glu Met Tyr Asp Gly Gln Tyr His Thr Ser Glu Leu Leu Gly Ile 
                325                 330                 335     


Ala Leu Ser Asp Gly Glu Lys Gly Tyr Phe Ala Pro Ala Asp Ile Ala 
            340                 345                 350         


Phe Gln Ser Lys Asp Phe Cys Ser Trp Leu Glu Asn Ala Thr Asn Lys 
        355                 360                 365             


Lys Tyr Leu Ala Asp Ser Lys Ala Thr Gln Ala Val Ser Arg Lys His 
    370                 375                 380                 


Asn Val Asn Val His Gly Val Glu Phe Asp Leu Leu Leu Ala Ala Tyr 
385                 390                 395                 400 


Ile Val Asn Pro Ala Ile Ser Ser Glu Asp Val Ala Ala Ile Ala Lys 
                405                 410                 415     


Glu Phe Gly Tyr Phe Asn Leu Leu Thr Asn Asp Ser Val Tyr Gly Lys 
            420                 425                 430         


Gly Ala Lys Lys Thr Ala Pro Glu Ile Glu Lys Ile Ala Glu His Ala 
        435                 440                 445             


Val Arg Lys Ala Arg Ala Ile Trp Asp Leu Lys Glu Lys Leu Glu Val 
    450                 455                 460                 


Lys Leu Glu Glu Asn Glu Gln Tyr Ala Leu Tyr Lys Glu Ile Glu Leu 
465                 470                 475                 480 


Pro Leu Ala Ser Ile Leu Gly Thr Met Glu Ser Asp Gly Val Leu Val 
                485                 490                 495     


Asp Lys Gln Ile Leu Val Glu Met Gly His Glu Leu Asn Ile Lys Leu 
            500                 505                 510         


Arg Ala Ile Glu Gln Asp Ile Tyr Ala Leu Ala Gly Glu Thr Phe Asn 
        515                 520                 525             


Ile Asn Ser Pro Lys Gln Leu Gly Val Ile Leu Phe Glu Lys Ile Gly 
    530                 535                 540                 


Leu Thr Pro Ile Lys Lys Thr Lys Thr Gly Tyr Ser Thr Ala Ala Asp 
545                 550                 555                 560 


Val Leu Glu Lys Leu Ala Ser Glu His Glu Ile Ile Glu Gln Ile Leu 
                565                 570                 575     


Leu Tyr Arg Gln Leu Gly Lys Leu Asn Ser Thr Tyr Ile Glu Gly Leu 
            580                 585                 590         


Leu Lys Glu Ile His Glu Asp Asp Gly Lys Ile His Thr Arg Tyr Gln 
        595                 600                 605             


Gln Ala Leu Thr Ser Thr Gly Arg Leu Ser Ser Ile Asn Pro Asn Leu 
    610                 615                 620                 


Gln Asn Ile Pro Val Arg Leu Glu Glu Gly Arg Lys Ile Arg Lys Ala 
625                 630                 635                 640 


Phe Val Pro Ser Gln Pro Gly Trp Val Met Phe Ala Ala Asp Tyr Ser 
                645                 650                 655     


Gln Ile Glu Leu Arg Val Leu Ala His Met Ser Glu Asp Glu Asn Leu 
            660                 665                 670         


Val Glu Ala Phe Asn Asn Asp Leu Asp Ile His Thr Lys Thr Ala Met 
        675                 680                 685             


Asp Val Phe His Val Glu Gln Glu Ala Val Thr Ser Asp Met Arg Arg 
    690                 695                 700                 


Ala Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly Ile Ser Asp Tyr 
705                 710                 715                 720 


Gly Leu Ser Gln Asn Leu Asp Ile Thr Arg Lys Glu Ala Ala Thr Phe 
                725                 730                 735     


Ile Glu Asn Tyr Leu Asn Ser Phe Pro Gly Val Lys Gly Tyr Met Asp 
            740                 745                 750         


Asp Ile Val Gln Asp Ala Lys Gln Thr Gly Tyr Val Thr Thr Ile Leu 
        755                 760                 765             


Asn Arg Arg Arg Tyr Leu Pro Glu Ile Thr Ser Ser Asn Phe Asn Leu 
    770                 775                 780                 


Arg Ser Phe Ala Glu Arg Thr Ala Met Asn Thr Pro Ile Gln Gly Ser 
785                 790                 795                 800 


Ala Ala Asp Ile Ile Lys Lys Ala Met Ile Asp Met Ala Glu Arg Leu 
                805                 810                 815     


Ile Ser Glu Asn Met Gln Thr Lys Met Leu Leu Gln Val His Asp Glu 
            820                 825                 830         


Leu Ile Phe Glu Ala Pro Pro Glu Glu Ile Ala Met Leu Glu Lys Ile 
        835                 840                 845             


Val Pro Glu Val Met Glu Asn Ala Ile Lys Leu Ile Val Pro Leu Lys 
    850                 855                 860                 


Val Asp Tyr Ala Phe Gly Ser Ser Trp Tyr Asp Thr Lys 
865                 870                 875         


<210>  4
<211>  2634
<212>  DNA
<213>  Psychrobacillus sp.

<400>  4
atgtatttgt caaccgagaa aatcctatta ttagacggca atagtttggc ataccgagct       60

ttttttgccc tacctttatt aacaaatgaa catggaatac atacaaacgc agtatatggc      120

tttacaatga tgctacaaaa aattatggat gaagaaaatc ctactcatat gctcgtggca      180

tttgatgccg ggaaaacgac cttccgtcac tctacttttg gggattataa aggtggaaga      240

caaaaaacac caccagaact atcggaacaa ttcccttata tacgcaagtt aatcgatgct      300

tatggtatta agcgatacga actggaaatg tacgaagcag acgatattat cggtacttta      360

agcaagcgtg cagacgaaaa agggcagcaa gttgtaattg tctcaggtga taaagattta      420

acacaactag ctacagataa aacaactgtg tatatcacaa gaaaaggcat aaccgatatt      480

gaaaaatata cacctgaaca tgtacaagaa aagtatggct taactccatt acagattata      540

gacatgaaag gtttaatggg agatgcttct gataatattc caggagttcc tggtgtcgga      600

gaaaaaacag ctattaagct tttaaaagaa catggttcgg tagaggattt atataaagca      660

cttgatacag ttagtggtgt taaactaaag gaaaaactca tcgccaacga agagcaggca      720

attatgagta aggcattagc tacgattgaa acagctgcac cgatacagat ttctatagac      780

gatctttcat atactggtcc taatatggaa gaagtaattg aagtttggaa ggaactagct      840

tttaaaactc ttcttgagaa atctgactat atttctgagg aatccgaaac tacagaagta      900

gcattcgaga ttgttgaaga aattgactct acaatattag ataaagtaat gtcagtccat      960

ttagaaatgt atgatgggca atatcataca agcgaattat taggtattgc tttatcagat     1020

ggagaaaagg gttattttgc tcctgctgat atagcttttc aatcgaagga tttttgttct     1080

tggttagaaa atgctacgaa taaaaagtat ttagcagact ccaaagcaac acaagcagtg     1140

agtagaaaac ataatgtgaa tgtacatgga gtggaattcg accttctttt agcagcgtat     1200

atagtaaatc ctgctatctc ttcagaggat gttgctgcta ttgctaaaga atttggatat     1260

tttaacttgc tgacaaacga tagtgtttat gggaaaggtg ccaaaaaaac cgcacctgaa     1320

atcgagaaaa ttgcagaaca tgccgtaaga aaagcaaggg ctatttggga cttgaaagaa     1380

aagttagaag taaaactgga agaaaatgaa caatatgcgt tgtataaaga aatagagcta     1440

ccgcttgcat ctatccttgg tacgatggaa tcagatgggg tgctggtgga taaacaaatt     1500

cttgtagaaa tgggtcatga gcttaatatt aagttacgag cgattgaaca agacatttat     1560

gcgttagctg gtgaaacgtt taatattaat tcacctaaac aattaggtgt aatactattt     1620

gaaaaaattg gtcttacccc tattaaaaag acaaaaacgg gctattcaac tgcagcagat     1680

gttttggaaa aactagcaag tgaacatgaa ataatagagc aaattttact atatcgtcaa     1740

ttaggtaaac tcaattccac atatatcgaa ggattattaa aagagattca tgaagatgat     1800

gggaagatcc atacccgata tcaacaagcc ctaacttcaa ctgggcgttt gagttcgatc     1860

aatccaaacc ttcaaaatat accagttcgt ttagaagaag gtagaaaaat acgtaaagcc     1920

tttgttcctt cacaaccggg atgggtaatg tttgcggcgg attactctca aattgaattg     1980

cgtgttcttg cccatatgtc tgaggatgaa aacctggtag aagcttttaa taatgatctg     2040

gatattcata ctaaaacggc tatggatgta ttccatgtgg agcaggaagc agtaacgtcc     2100

gatatgcgcc gtgctgctaa ggcagttaac tttgggattg tgtatggtat tagtgattat     2160

ggtttatcac aaaacctaga tattactaga aaagaagcgg cgacatttat cgagaattat     2220

ttaaatagct tcccaggtgt aaaaggatat atggatgata tcgttcaaga tgcgaaacaa     2280

acaggctacg ttacaacaat tttgaataga cgaagatatt tgcctgaaat aacaagttct     2340

aactttaatc tccgcagttt tgcagaacgt actgctatga atacaccaat tcaagggagt     2400

gcagccgata ttattaaaaa agcaatgatc gatatggcgg aaagattaat atcagaaaat     2460

atgcagacca aaatgctact acaagtacat gatgaattaa tttttgaggc tccaccagag     2520

gaaattgcaa tgctagaaaa aatagtgcca gaggtgatgg aaaacgctat taaactgatt     2580

gtacctttga aagtggatta tgcctttggt tcatcttggt atgacacgaa gtag           2634


<210>  5
<211>  297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence containing a 5'-3' exonuclease functional domain of DNA 
       polymerase I isolated from a Psychrobacillus sp.

<400>  5

Met Tyr Leu Ser Thr Glu Lys Ile Leu Leu Leu Asp Gly Asn Ser Leu 
1               5                   10                  15      


Ala Tyr Arg Ala Phe Phe Ala Leu Pro Leu Leu Thr Asn Glu His Gly 
            20                  25                  30          


Ile His Thr Asn Ala Val Tyr Gly Phe Thr Met Met Leu Gln Lys Ile 
        35                  40                  45              


Met Asp Glu Glu Asn Pro Thr His Met Leu Val Ala Phe Asp Ala Gly 
    50                  55                  60                  


Lys Thr Thr Phe Arg His Ser Thr Phe Gly Asp Tyr Lys Gly Gly Arg 
65                  70                  75                  80  


Gln Lys Thr Pro Pro Glu Leu Ser Glu Gln Phe Pro Tyr Ile Arg Lys 
                85                  90                  95      


Leu Ile Asp Ala Tyr Gly Ile Lys Arg Tyr Glu Leu Glu Met Tyr Glu 
            100                 105                 110         


Ala Asp Asp Ile Ile Gly Thr Leu Ser Lys Arg Ala Asp Glu Lys Gly 
        115                 120                 125             


Gln Gln Val Val Ile Val Ser Gly Asp Lys Asp Leu Thr Gln Leu Ala 
    130                 135                 140                 


Thr Asp Lys Thr Thr Val Tyr Ile Thr Arg Lys Gly Ile Thr Asp Ile 
145                 150                 155                 160 


Glu Lys Tyr Thr Pro Glu His Val Gln Glu Lys Tyr Gly Leu Thr Pro 
                165                 170                 175     


Leu Gln Ile Ile Asp Met Lys Gly Leu Met Gly Asp Ala Ser Asp Asn 
            180                 185                 190         


Ile Pro Gly Val Pro Gly Val Gly Glu Lys Thr Ala Ile Lys Leu Leu 
        195                 200                 205             


Lys Glu His Gly Ser Val Glu Asp Leu Tyr Lys Ala Leu Asp Thr Val 
    210                 215                 220                 


Ser Gly Val Lys Leu Lys Glu Lys Leu Ile Ala Asn Glu Glu Gln Ala 
225                 230                 235                 240 


Ile Met Ser Lys Ala Leu Ala Thr Ile Glu Thr Ala Ala Pro Ile Gln 
                245                 250                 255     


Ile Ser Ile Asp Asp Leu Ser Tyr Thr Gly Pro Asn Met Glu Glu Val 
            260                 265                 270         


Ile Glu Val Trp Lys Glu Leu Ala Phe Lys Thr Leu Leu Glu Lys Ser 
        275                 280                 285             


Asp Tyr Ile Ser Glu Glu Ser Glu Thr 
    290                 295         


<210>  6
<211>  605
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated DNA polymerase of Aliivibrio salmonicida

<400>  6

Val Asp Arg Ser Lys Tyr Glu Thr Ile Phe Thr Lys Glu Ala Phe Ser 
1               5                   10                  15      


Ala Trp Leu Glu Lys Val Asn Asn Ala Glu Val Thr Ala Phe Asp Thr 
            20                  25                  30          


Glu Thr Asp Ser Leu Asp Tyr Met Val Ala Asn Leu Ile Gly Leu Ser 
        35                  40                  45              


Phe Ser Val Glu Glu Gly Glu Ala Ala Tyr Val Pro Val Ala His Asp 
    50                  55                  60                  


Tyr Leu Asp Ala Pro Glu Gln Leu Asp Arg Asp Trp Val Leu Ala Gln 
65                  70                  75                  80  


Leu Lys Pro Tyr Leu Glu Asp Glu Thr Lys Ala Lys Val Gly Gln Asn 
                85                  90                  95      


Leu Lys Tyr Asp Ala Ser Val Leu Ala Arg Tyr Asp Ile Glu Met Lys 
            100                 105                 110         


Gly Ile Lys Phe Asp Thr Met Leu Glu Ser Tyr Val Tyr Asn Ser Val 
        115                 120                 125             


Ala Gly Lys His Asn Met Asp Ser Leu Ala Leu Arg Tyr Leu Gln His 
    130                 135                 140                 


Asn Thr Ile Ser Phe Glu Glu Ile Ala Gly Lys Gly Lys Lys Gln Leu 
145                 150                 155                 160 


Thr Phe Asn Gln Ile Ala Leu Glu Glu Ala Ala Pro Tyr Ala Ala Glu 
                165                 170                 175     


Asp Ala Asp Ile Thr Leu Arg Leu His Asn Val Leu His Ala Lys Leu 
            180                 185                 190         


Val Thr Asp Glu Lys Leu Asn Ala Val Phe Thr Asp Ile Glu Leu Pro 
        195                 200                 205             


Leu Ile Ser Val Leu Ser Arg Met Glu Arg Lys Gly Val Tyr Ile Asp 
    210                 215                 220                 


Asp Met Leu Leu Ser Ala Gln Ser Leu Glu Ile Gly Gln Arg Leu Asp 
225                 230                 235                 240 


Glu Leu Glu Thr Ala Ser Phe Glu Val Ala Gly Gln Glu Phe Asn Met 
                245                 250                 255     


Asn Ser Pro Lys Gln Leu Gln Thr Ile Leu Phe Glu Lys Met Glu Leu 
            260                 265                 270         


Pro Val Ile Lys Lys Thr Pro Ser Gly Ala Ala Ser Thr Asn Glu Glu 
        275                 280                 285             


Val Leu Gln Glu Leu Ala Leu Glu Tyr Glu Leu Pro Lys Leu Ile Leu 
    290                 295                 300                 


Glu Tyr Arg Gly Leu Ala Lys Leu Lys Ser Thr Tyr Thr Asp Lys Leu 
305                 310                 315                 320 


Pro Lys Met Ile Asn Pro Ser Thr Gly Arg Val His Thr Ser Tyr His 
                325                 330                 335     


Gln Ala Val Thr Ala Thr Gly Arg Leu Ser Ser Thr Asp Pro Asn Leu 
            340                 345                 350         


Gln Asn Ile Pro Ile Arg Asn Lys Glu Gly Arg Arg Ile Arg Gln Ala 
        355                 360                 365             


Phe Val Ala Pro His Gly Trp Lys Ile Leu Ala Val Asp Tyr Ser Gln 
    370                 375                 380                 


Ile Glu Leu Arg Ile Met Ala His Leu Ser Gln Asp Arg Ala Leu Leu 
385                 390                 395                 400 


Glu Ala Phe Ser Ala Gly Lys Asp Ile His Ala Ala Thr Ala Ala Glu 
                405                 410                 415     


Val Lys Gly Val Ser Ile Glu Glu Val Thr Ser Glu Asp Arg Arg Arg 
            420                 425                 430         


Ala Lys Ala Ile Asn Phe Gly Leu Ile Tyr Gly Met Ser Ala Phe Gly 
        435                 440                 445             


Leu Ala Lys Gln Ile Gly Ile Ser Arg Gly Glu Ala Gln Asp Tyr Met 
    450                 455                 460                 


Asn Val Tyr Phe Glu Arg Tyr Pro Gly Val Met Gln Tyr Met Glu Glu 
465                 470                 475                 480 


Thr Arg Leu Leu Ala Thr Glu Gln Gly Tyr Val Glu Thr Leu Tyr Gly 
                485                 490                 495     


Arg Arg Leu Tyr Leu Pro Glu Ile Asn Ala Arg Asn Ala Ile Arg Arg 
            500                 505                 510         


Lys Ala Ala Glu Arg Ala Ala Ile Asn Ala Pro Met Gln Gly Thr Ala 
        515                 520                 525             


Ala Asp Ile Ile Lys Lys Ala Met Ile Leu Val Asp Asn Trp Ile Glu 
    530                 535                 540                 


Ala Glu Gly Thr Gly Arg Val Asn Leu Leu Met Gln Val His Asp Glu 
545                 550                 555                 560 


Leu Val Phe Glu Val Lys Glu Asp Glu Leu Glu Ala Ile Thr Lys Gln 
                565                 570                 575     


Val Thr Ala Leu Met Glu Ala Ala Val Ser Leu Asp Val Pro Leu Ile 
            580                 585                 590         


Ala Glu Ser Gly Phe Gly Asp Asn Trp Asp Glu Ala His 
        595                 600                 605 


<210>  7
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
caccacagaa gtagcattcg agattgtt                                          28


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
ttacttcgtg tcataccaag atgaacc                                           27


<210>  9
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  molecular beacon template


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  labeled with the fluorophore Dabcyl

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  labeled with the fluorophore FAM

<400>  9
ggcccgtagg aggaaaggac atcttctagc atacgggccg tcaagttcat ggccagtcaa       60

gtcgtcagaa atttcgcacc ac                                                82


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
gtggtgcgaa atttctgac                                                    19


<210>  11
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cold primer

<400>  11
tatccaccaa tactaccct                                                    19


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reporter strand


<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  labeled with TAMRA fluorophore at 3' end

<400>  12
cgatactttg tccactcaat                                                   20


<210>  13
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template strand


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  labeled at 5' end with the Black Hole Quencher 2

<400>  13
attgagtgga caaagtatcg tagggtagta ttggtggata                             40


<210>  14
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template strand

<400>  14
attgagtgga gatagtatcg tagggtagta ttggtggata                             40


<210>  15
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  labeled at 5' end with the fluorophore FAM

<400>  15
tatccaccaa tactaccct                                                    19


<210>  16
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template

<400>  16
cacaccaaca cacacaacac caacaaccac acaacaccac cacaaccaac acacaacacc       60

gtgtgaattc ggcactggcc gtcgtatgct cttggttgta                            100


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  labeled at 5-end with the fluorophore FAM

<400>  17
tacaaccaag agcatacgac                                                   20


