               SEQUENCE LISTING

<110> Chang Gung University
      Linkou Chang Gung Memorial Hospital
      CHANG, Yu-Sun

<120> METHOD FOR CANCER DIAGNOSIS AND PROGNOSIS

<130> P3006-PCT

<150> US62309766
<151> 2016-03-17

<160> 8

<170> BiSSAP 1.3

<210> 1
<211> 339
<212> PRT
<213> Artificial Sequence

<220> 
<223> ANXA2 polypeptide

<400> 1
Met Ser Thr Val His Glu Ile Leu Cys Lys Leu Ser Leu Glu Gly Asp 
1               5                   10                  15      
His Ser Thr Pro Pro Ser Ala Tyr Gly Ser Val Lys Ala Tyr Thr Asn 
            20                  25                  30          
Phe Asp Ala Glu Arg Asp Ala Leu Asn Ile Glu Thr Ala Ile Lys Thr 
        35                  40                  45              
Lys Gly Val Asp Glu Val Thr Ile Val Asn Ile Leu Thr Asn Arg Ser 
    50                  55                  60                  
Asn Ala Gln Arg Gln Asp Ile Ala Phe Ala Tyr Gln Arg Arg Thr Lys 
65                  70                  75                  80  
Lys Glu Leu Ala Ser Ala Leu Lys Ser Ala Leu Ser Gly His Leu Glu 
                85                  90                  95      
Thr Val Ile Leu Gly Leu Leu Lys Thr Pro Ala Gln Tyr Asp Ala Ser 
            100                 105                 110         
Glu Leu Lys Ala Ser Met Lys Gly Leu Gly Thr Asp Glu Asp Ser Leu 
        115                 120                 125             
Ile Glu Ile Ile Cys Ser Arg Thr Asn Gln Glu Leu Gln Glu Ile Asn 
    130                 135                 140                 
Arg Val Tyr Lys Glu Met Tyr Lys Thr Asp Leu Glu Lys Asp Ile Ile 
145                 150                 155                 160 
Ser Asp Thr Ser Gly Asp Phe Arg Lys Leu Met Val Ala Leu Ala Lys 
                165                 170                 175     
Gly Arg Arg Ala Glu Asp Gly Ser Val Ile Asp Tyr Glu Leu Ile Asp 
            180                 185                 190         
Gln Asp Ala Arg Asp Leu Tyr Asp Ala Gly Val Lys Arg Lys Gly Thr 
        195                 200                 205             
Asp Val Pro Lys Trp Ile Ser Ile Met Thr Glu Arg Ser Val Pro His 
    210                 215                 220                 
Leu Gln Lys Val Phe Asp Arg Tyr Lys Ser Tyr Ser Pro Tyr Asp Met 
225                 230                 235                 240 
Leu Glu Ser Ile Arg Lys Glu Val Lys Gly Asp Leu Glu Asn Ala Phe 
                245                 250                 255     
Leu Asn Leu Val Gln Cys Ile Gln Asn Lys Pro Leu Tyr Phe Ala Asp 
            260                 265                 270         
Arg Leu Tyr Asp Ser Met Lys Gly Lys Gly Thr Arg Asp Lys Val Leu 
        275                 280                 285             
Ile Arg Ile Met Val Ser Arg Ser Glu Val Asp Met Leu Lys Ile Arg 
    290                 295                 300                 
Ser Glu Phe Lys Arg Lys Tyr Gly Lys Ser Leu Tyr Tyr Tyr Ile Gln 
305                 310                 315                 320 
Gln Asp Thr Lys Gly Asp Tyr Gln Lys Ala Leu Leu Tyr Leu Cys Gly 
                325                 330                 335     
Gly Asp Asp 
            

<210> 2
<211> 654
<212> PRT
<213> Artificial Sequence

<220> 
<223> HSPA5 polypeptide

<400> 2
Met Lys Leu Ser Leu Val Ala Ala Met Leu Leu Leu Leu Ser Ala Ala 
1               5                   10                  15      
Arg Ala Glu Glu Glu Asp Lys Lys Glu Asp Val Gly Thr Val Val Gly 
            20                  25                  30          
Ile Asp Leu Gly Thr Thr Tyr Ser Cys Val Gly Val Phe Lys Asn Gly 
        35                  40                  45              
Arg Val Glu Ile Ile Ala Asn Asp Gln Gly Asn Arg Ile Thr Pro Ser 
    50                  55                  60                  
Tyr Val Ala Phe Thr Pro Glu Gly Glu Arg Leu Ile Gly Asp Ala Ala 
65                  70                  75                  80  
Lys Asn Gln Leu Thr Ser Asn Pro Glu Asn Thr Val Phe Asp Ala Lys 
                85                  90                  95      
Arg Leu Ile Gly Arg Thr Trp Asn Asp Pro Ser Val Gln Gln Asp Ile 
            100                 105                 110         
Lys Phe Leu Pro Phe Lys Val Val Glu Lys Lys Thr Lys Pro Tyr Ile 
        115                 120                 125             
Gln Val Asp Ile Gly Gly Gly Gln Thr Lys Thr Phe Ala Pro Glu Glu 
    130                 135                 140                 
Ile Ser Ala Met Val Leu Thr Lys Met Lys Glu Thr Ala Glu Ala Tyr 
145                 150                 155                 160 
Leu Gly Lys Lys Val Thr His Ala Val Val Thr Val Pro Ala Tyr Phe 
                165                 170                 175     
Asn Asp Ala Gln Arg Gln Ala Thr Lys Asp Ala Gly Thr Ile Ala Gly 
            180                 185                 190         
Leu Asn Val Met Arg Ile Ile Asn Glu Pro Thr Ala Ala Ala Ile Ala 
        195                 200                 205             
Tyr Gly Leu Asp Lys Arg Glu Gly Glu Lys Asn Ile Leu Val Phe Asp 
    210                 215                 220                 
Leu Gly Gly Gly Thr Phe Asp Val Ser Leu Leu Thr Ile Asp Asn Gly 
225                 230                 235                 240 
Val Phe Glu Val Val Ala Thr Asn Gly Asp Thr His Leu Gly Gly Glu 
                245                 250                 255     
Asp Phe Asp Gln Arg Val Met Glu His Phe Ile Lys Leu Tyr Lys Lys 
            260                 265                 270         
Lys Thr Gly Lys Asp Val Arg Lys Asp Asn Arg Ala Val Gln Lys Leu 
        275                 280                 285             
Arg Arg Glu Val Glu Lys Ala Lys Arg Ala Leu Ser Ser Gln His Gln 
    290                 295                 300                 
Ala Arg Ile Glu Ile Glu Ser Phe Tyr Glu Gly Glu Asp Phe Ser Glu 
305                 310                 315                 320 
Thr Leu Thr Arg Ala Lys Phe Glu Glu Leu Asn Met Asp Leu Phe Arg 
                325                 330                 335     
Ser Thr Met Lys Pro Val Gln Lys Val Leu Glu Asp Ser Asp Leu Lys 
            340                 345                 350         
Lys Ser Asp Ile Asp Glu Ile Val Leu Val Gly Gly Ser Thr Arg Ile 
        355                 360                 365             
Pro Lys Ile Gln Gln Leu Val Lys Glu Phe Phe Asn Gly Lys Glu Pro 
    370                 375                 380                 
Ser Arg Gly Ile Asn Pro Asp Glu Ala Val Ala Tyr Gly Ala Ala Val 
385                 390                 395                 400 
Gln Ala Gly Val Leu Ser Gly Asp Gln Asp Thr Gly Asp Leu Val Leu 
                405                 410                 415     
Leu Asp Val Cys Pro Leu Thr Leu Gly Ile Glu Thr Val Gly Gly Val 
            420                 425                 430         
Met Thr Lys Leu Ile Pro Arg Asn Thr Val Val Pro Thr Lys Lys Ser 
        435                 440                 445             
Gln Ile Phe Ser Thr Ala Ser Asp Asn Gln Pro Thr Val Thr Ile Lys 
    450                 455                 460                 
Val Tyr Glu Gly Glu Arg Pro Leu Thr Lys Asp Asn His Leu Leu Gly 
465                 470                 475                 480 
Thr Phe Asp Leu Thr Gly Ile Pro Pro Ala Pro Arg Gly Val Pro Gln 
                485                 490                 495     
Ile Glu Val Thr Phe Glu Ile Asp Val Asn Gly Ile Leu Arg Val Thr 
            500                 505                 510         
Ala Glu Asp Lys Gly Thr Gly Asn Lys Asn Lys Ile Thr Ile Thr Asn 
        515                 520                 525             
Asp Gln Asn Arg Leu Thr Pro Glu Glu Ile Glu Arg Met Val Asn Asp 
    530                 535                 540                 
Ala Glu Lys Phe Ala Glu Glu Asp Lys Lys Leu Lys Glu Arg Ile Asp 
545                 550                 555                 560 
Thr Arg Asn Glu Leu Glu Ser Tyr Ala Tyr Ser Leu Lys Asn Gln Ile 
                565                 570                 575     
Gly Asp Lys Glu Lys Leu Gly Gly Lys Leu Ser Ser Glu Asp Lys Glu 
            580                 585                 590         
Thr Met Glu Lys Ala Val Glu Glu Lys Ile Glu Trp Leu Glu Ser His 
        595                 600                 605             
Gln Asp Ala Asp Ile Glu Asp Phe Lys Ala Lys Lys Lys Glu Leu Glu 
    610                 615                 620                 
Glu Ile Val Gln Pro Ile Ile Ser Lys Leu Tyr Gly Ser Ala Gly Pro 
625                 630                 635                 640 
Pro Pro Thr Gly Glu Glu Asp Thr Ala Glu Lys Asp Glu Leu 
                645                 650                 

<210> 3
<211> 644
<212> PRT
<213> Artificial Sequence

<220> 
<223> KNG1 polypeptide

<400> 3
Met Lys Leu Ile Thr Ile Leu Phe Leu Cys Ser Arg Leu Leu Leu Ser 
1               5                   10                  15      
Leu Thr Gln Glu Ser Gln Ser Glu Glu Ile Asp Cys Asn Asp Lys Asp 
            20                  25                  30          
Leu Phe Lys Ala Val Asp Ala Ala Leu Lys Lys Tyr Asn Ser Gln Asn 
        35                  40                  45              
Gln Ser Asn Asn Gln Phe Val Leu Tyr Arg Ile Thr Glu Ala Thr Lys 
    50                  55                  60                  
Thr Val Gly Ser Asp Thr Phe Tyr Ser Phe Lys Tyr Glu Ile Lys Glu 
65                  70                  75                  80  
Gly Asp Cys Pro Val Gln Ser Gly Lys Thr Trp Gln Asp Cys Glu Tyr 
                85                  90                  95      
Lys Asp Ala Ala Lys Ala Ala Thr Gly Glu Cys Thr Ala Thr Val Gly 
            100                 105                 110         
Lys Arg Ser Ser Thr Lys Phe Ser Val Ala Thr Gln Thr Cys Gln Ile 
        115                 120                 125             
Thr Pro Ala Glu Gly Pro Val Val Thr Ala Gln Tyr Asp Cys Leu Gly 
    130                 135                 140                 
Cys Val His Pro Ile Ser Thr Gln Ser Pro Asp Leu Glu Pro Ile Leu 
145                 150                 155                 160 
Arg His Gly Ile Gln Tyr Phe Asn Asn Asn Thr Gln His Ser Ser Leu 
                165                 170                 175     
Phe Met Leu Asn Glu Val Lys Arg Ala Gln Arg Gln Val Val Ala Gly 
            180                 185                 190         
Leu Asn Phe Arg Ile Thr Tyr Ser Ile Val Gln Thr Asn Cys Ser Lys 
        195                 200                 205             
Glu Asn Phe Leu Phe Leu Thr Pro Asp Cys Lys Ser Leu Trp Asn Gly 
    210                 215                 220                 
Asp Thr Gly Glu Cys Thr Asp Asn Ala Tyr Ile Asp Ile Gln Leu Arg 
225                 230                 235                 240 
Ile Ala Ser Phe Ser Gln Asn Cys Asp Ile Tyr Pro Gly Lys Asp Phe 
                245                 250                 255     
Val Gln Pro Pro Thr Lys Ile Cys Val Gly Cys Pro Arg Asp Ile Pro 
            260                 265                 270         
Thr Asn Ser Pro Glu Leu Glu Glu Thr Leu Thr His Thr Ile Thr Lys 
        275                 280                 285             
Leu Asn Ala Glu Asn Asn Ala Thr Phe Tyr Phe Lys Ile Asp Asn Val 
    290                 295                 300                 
Lys Lys Ala Arg Val Gln Val Val Ala Gly Lys Lys Tyr Phe Ile Asp 
305                 310                 315                 320 
Phe Val Ala Arg Glu Thr Thr Cys Ser Lys Glu Ser Asn Glu Glu Leu 
                325                 330                 335     
Thr Glu Ser Cys Glu Thr Lys Lys Leu Gly Gln Ser Leu Asp Cys Asn 
            340                 345                 350         
Ala Glu Val Tyr Val Val Pro Trp Glu Lys Lys Ile Tyr Pro Thr Val 
        355                 360                 365             
Asn Cys Gln Pro Leu Gly Met Ile Ser Leu Met Lys Arg Pro Pro Gly 
    370                 375                 380                 
Phe Ser Pro Phe Arg Ser Ser Arg Ile Gly Glu Ile Lys Glu Glu Thr 
385                 390                 395                 400 
Thr Val Ser Pro Pro His Thr Ser Met Ala Pro Ala Gln Asp Glu Glu 
                405                 410                 415     
Arg Asp Ser Gly Lys Glu Gln Gly His Thr Arg Arg His Asp Trp Gly 
            420                 425                 430         
His Glu Lys Gln Arg Lys His Asn Leu Gly His Gly His Lys His Glu 
        435                 440                 445             
Arg Asp Gln Gly His Gly His Gln Arg Gly His Gly Leu Gly His Gly 
    450                 455                 460                 
His Glu Gln Gln His Gly Leu Gly His Gly His Lys Phe Lys Leu Asp 
465                 470                 475                 480 
Asp Asp Leu Glu His Gln Gly Gly His Val Leu Asp His Gly His Lys 
                485                 490                 495     
His Lys His Gly His Gly His Gly Lys His Lys Asn Lys Gly Lys Lys 
            500                 505                 510         
Asn Gly Lys His Asn Gly Trp Lys Thr Glu His Leu Ala Ser Ser Ser 
        515                 520                 525             
Glu Asp Ser Thr Thr Pro Ser Ala Gln Thr Gln Glu Lys Thr Glu Gly 
    530                 535                 540                 
Pro Thr Pro Ile Pro Ser Leu Ala Lys Pro Gly Val Thr Val Thr Phe 
545                 550                 555                 560 
Ser Asp Phe Gln Asp Ser Asp Leu Ile Ala Thr Met Met Pro Pro Ile 
                565                 570                 575     
Ser Pro Ala Pro Ile Gln Ser Asp Asp Asp Trp Ile Pro Asp Ile Gln 
            580                 585                 590         
Ile Asp Pro Asn Gly Leu Ser Phe Asn Pro Ile Ser Asp Phe Pro Asp 
        595                 600                 605             
Thr Thr Ser Pro Lys Cys Pro Gly Arg Pro Trp Lys Ser Val Ser Glu 
    610                 615                 620                 
Ile Asn Pro Thr Thr Gln Met Lys Glu Ser Tyr Tyr Phe Asp Leu Thr 
625                 630                 635                 640 
Asp Gly Leu Ser 
                

<210> 4
<211> 469
<212> PRT
<213> Artificial Sequence

<220> 
<223> MMP1 polypeptide

<400> 4
Met His Ser Phe Pro Pro Leu Leu Leu Leu Leu Phe Trp Gly Val Val 
1               5                   10                  15      
Ser His Ser Phe Pro Ala Thr Leu Glu Thr Gln Glu Gln Asp Val Asp 
            20                  25                  30          
Leu Val Gln Lys Tyr Leu Glu Lys Tyr Tyr Asn Leu Lys Asn Asp Gly 
        35                  40                  45              
Arg Gln Val Glu Lys Arg Arg Asn Ser Gly Pro Val Val Glu Lys Leu 
    50                  55                  60                  
Lys Gln Met Gln Glu Phe Phe Gly Leu Lys Val Thr Gly Lys Pro Asp 
65                  70                  75                  80  
Ala Glu Thr Leu Lys Val Met Lys Gln Pro Arg Cys Gly Val Pro Asp 
                85                  90                  95      
Val Ala Gln Phe Val Leu Thr Glu Gly Asn Pro Arg Trp Glu Gln Thr 
            100                 105                 110         
His Leu Thr Tyr Arg Ile Glu Asn Tyr Thr Pro Asp Leu Pro Arg Ala 
        115                 120                 125             
Asp Val Asp His Ala Ile Glu Lys Ala Phe Gln Leu Trp Ser Asn Val 
    130                 135                 140                 
Thr Pro Leu Thr Phe Thr Lys Val Ser Glu Gly Gln Ala Asp Ile Met 
145                 150                 155                 160 
Ile Ser Phe Val Arg Gly Asp His Arg Asp Asn Ser Pro Phe Asp Gly 
                165                 170                 175     
Pro Gly Gly Asn Leu Ala His Ala Phe Gln Pro Gly Pro Gly Ile Gly 
            180                 185                 190         
Gly Asp Ala His Phe Asp Glu Asp Glu Arg Trp Thr Asn Asn Phe Arg 
        195                 200                 205             
Glu Tyr Asn Leu His Arg Val Ala Ala His Glu Leu Gly His Ser Leu 
    210                 215                 220                 
Gly Leu Ser His Ser Thr Asp Ile Gly Ala Leu Met Tyr Pro Ser Tyr 
225                 230                 235                 240 
Thr Phe Ser Gly Asp Val Gln Leu Ala Gln Asp Asp Ile Asp Gly Ile 
                245                 250                 255     
Gln Ala Ile Tyr Gly Arg Ser Gln Asn Pro Val Gln Pro Ile Gly Pro 
            260                 265                 270         
Gln Thr Pro Lys Ala Cys Asp Ser Lys Leu Thr Phe Asp Ala Ile Thr 
        275                 280                 285             
Thr Ile Arg Gly Glu Val Met Phe Phe Lys Asp Arg Phe Tyr Met Arg 
    290                 295                 300                 
Thr Asn Pro Phe Tyr Pro Glu Val Glu Leu Asn Phe Ile Ser Val Phe 
305                 310                 315                 320 
Trp Pro Gln Leu Pro Asn Gly Leu Glu Ala Ala Tyr Glu Phe Ala Asp 
                325                 330                 335     
Arg Asp Glu Val Arg Phe Phe Lys Gly Asn Lys Tyr Trp Ala Val Gln 
            340                 345                 350         
Gly Gln Asn Val Leu His Gly Tyr Pro Lys Asp Ile Tyr Ser Ser Phe 
        355                 360                 365             
Gly Phe Pro Arg Thr Val Lys His Ile Asp Ala Ala Leu Ser Glu Glu 
    370                 375                 380                 
Asn Thr Gly Lys Thr Tyr Phe Phe Val Ala Asn Lys Tyr Trp Arg Tyr 
385                 390                 395                 400 
Asp Glu Tyr Lys Arg Ser Met Asp Pro Gly Tyr Pro Lys Met Ile Ala 
                405                 410                 415     
His Asp Phe Pro Gly Ile Gly His Lys Val Asp Ala Val Phe Met Lys 
            420                 425                 430         
Asp Gly Phe Phe Tyr Phe Phe His Gly Thr Arg Gln Tyr Lys Phe Asp 
        435                 440                 445             
Pro Lys Thr Lys Arg Ile Leu Thr Leu Gln Lys Ala Asn Ser Trp Phe 
    450                 455                 460                 
Asn Cys Arg Lys Asn 
465                 

<210> 5
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> SIS peptide for ANXA2

<400> 5
Gln Asp Ile Ala Phe Ala Tyr Gln Arg 
1               5                   

<210> 6
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> SIS peptide for HSPA5

<400> 6
Ile Thr Pro Ser Tyr Val Ala Phe Thr Pro Glu Gly Glu Arg 
1               5                   10                  

<210> 7
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> SIS peptide for KNG1

<400> 7
Thr Val Gly Ser Asp Thr Phe Tyr Ser Phe Lys 
1               5                   10      

<210> 8
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> SIS peptide for MMP1

<400> 8
Asp Ile Tyr Ser Ser Phe Gly Phe Pro Arg 
1               5                   10  

