                               SEQUENCE LISTING

<110> PRESIDENT AND FELLOWS OF HARVARD COLLEGE
 
<120> MUTANT CAS PROTEINS

<130> 010498.00911/WO

<140> PCT/US2017/022626
<141> 2017-03-16

<150> 62/310,018
<151> 2016-03-18

<160> 47    

<170> PatentIn version 3.5

<210> 1
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Cas9 cloning plasmid

<400> 1
ttttgctctt ctagtggcgg gtcagggtcg                                        30


<210> 2
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Cas9 cloning plasmid

<400> 2
ttttgctctt ctcta                                                        15


<210> 3
<211> 7338
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> dCas9-m4-golden gate compatible vector

<400> 3
gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta       60

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag      120

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc      180

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga      240

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat      300

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc      360

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct      420

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca      480

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat      540

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg      600

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg      660

agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg      720

actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgctatc      780

ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa      840

ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc      900

ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga      960

tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct     1020

aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa     1080

aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag     1140

tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg     1200

cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag     1260

ggggacctga acccagacaa cagcgatgtc gataaactct ttatccaact ggttcagact     1320

tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc     1380

ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg     1440

gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac     1500

tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac     1560

gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg     1620

gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag     1680

atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac     1740

ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc     1800

ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa     1860

ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta     1920

aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc     1980

caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc     2040

tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat     2100

gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag     2160

accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc     2220

atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac     2280

tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca     2340

gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc     2400

ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag     2460

attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg     2520

ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag     2580

aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg     2640

attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc     2700

aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga     2760

gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg     2820

aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca     2880

caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca     2940

gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg     3000

ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag     3060

aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg     3120

gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc     3180

tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat     3240

cggctctccg actacgacgt ggctgctatc gtgccccagt cttttctcaa agatgattct     3300

attgataata aagtgttgac aagatccgat aaagctagag ggaagagtga taacgtcccc     3360

tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg     3420

atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg     3480

gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg     3540

gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga     3600

gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag     3660

ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca     3720

gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga     3780

gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag     3840

gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca     3900

ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa     3960

atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag     4020

gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc     4080

ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac     4140

ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa     4200

gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga     4260

tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa     4320

aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa     4380

cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa     4440

tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat     4500

aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag     4560

caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt     4620

tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac     4680

ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata     4740

gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca     4800

attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct     4860

gaccccaaga agaagaggaa ggtgagtggt ggaggaagtt gaagagctat gtttagatat     4920

ccaaaccagg ctcttcttag aagaattcga tccctaccgg ttagtaatga gtttaaacgg     4980

gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca     5040

ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg     5100

gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg     5160

cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc     5220

caacgtcggg gcggcaggcc ctccatagtc ggtcgttcgg ctgcggcgag cggtatcagc     5280

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     5340

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     5400

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     5460

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     5520

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     5580

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     5640

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     5700

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     5760

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     5820

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     5880

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     5940

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     6000

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     6060

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     6120

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc     6180

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta     6240

gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga     6300

cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg     6360

cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc     6420

tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat     6480

cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag     6540

gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat     6600

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa     6660

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa     6720

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga     6780

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg     6840

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc     6900

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg     6960

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact     7020

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat     7080

atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt     7140

gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac tctcagtaca     7200

atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc     7260

gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc     7320

atgaagaatc tgcttagg                                                   7338


<210> 4
<211> 7338
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Cas9 golden gate compatible vector

<400> 4
gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta       60

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag      120

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc      180

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga      240

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat      300

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc      360

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct      420

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca      480

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat      540

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg      600

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg      660

agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg      720

actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgatatc      780

ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa      840

ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc      900

ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga      960

tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct     1020

aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa     1080

aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag     1140

tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg     1200

cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag     1260

ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact     1320

tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc     1380

ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg     1440

gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac     1500

tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac     1560

gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg     1620

gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag     1680

atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac     1740

ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc     1800

ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa     1860

ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta     1920

aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc     1980

caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc     2040

tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat     2100

gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag     2160

accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc     2220

atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac     2280

tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca     2340

gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc     2400

ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag     2460

attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg     2520

ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag     2580

aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg     2640

attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc     2700

aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga     2760

gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg     2820

aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca     2880

caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca     2940

gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg     3000

ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag     3060

aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg     3120

gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc     3180

tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat     3240

cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa agatgattct     3300

attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga taacgtcccc     3360

tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg     3420

atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg     3480

gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg     3540

gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga     3600

gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag     3660

ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca     3720

gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga     3780

gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag     3840

gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca     3900

ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa     3960

atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag     4020

gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc     4080

ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac     4140

ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa     4200

gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga     4260

tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa     4320

aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa     4380

cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa     4440

tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat     4500

aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag     4560

caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt     4620

tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac     4680

ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata     4740

gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca     4800

attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct     4860

gaccccaaga agaagaggaa ggtgagtggt ggaggaagtt gaagagctat gtttagatat     4920

ccaaaccagg ctcttcttag aagaattcga tccctaccgg ttagtaatga gtttaaacgg     4980

gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca     5040

ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg     5100

gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg     5160

cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc     5220

caacgtcggg gcggcaggcc ctccatagtc ggtcgttcgg ctgcggcgag cggtatcagc     5280

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     5340

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     5400

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     5460

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     5520

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     5580

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     5640

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     5700

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     5760

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     5820

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     5880

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     5940

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     6000

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     6060

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     6120

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc     6180

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta     6240

gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga     6300

cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg     6360

cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc     6420

tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat     6480

cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag     6540

gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat     6600

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa     6660

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa     6720

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga     6780

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg     6840

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc     6900

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg     6960

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact     7020

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat     7080

atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt     7140

gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac tctcagtaca     7200

atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc     7260

gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc     7320

atgaagaatc tgcttagg                                                   7338


<210> 5
<211> 9121
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> gRNA cloning vector pSB700-SAM

<400> 5
tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca       60

cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac      120

tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca      180

ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg      240

agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag      300

agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg      360

ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat      420

cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga      480

gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct      540

tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc      600

agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag      660

cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg      720

caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga      780

aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg      840

aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg      900

caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct      960

gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat     1020

cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca     1080

ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc     1140

aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag     1200

tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc     1260

aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg     1320

gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc     1380

cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc     1440

gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct     1500

ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa     1560

actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca     1620

gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt     1680

aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt     1740

ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta     1800

tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt     1860

actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct     1920

cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga     1980

cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag     2040

gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac     2100

aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg     2160

acagcagaga tccagtttat cattagtgaa cggatctcga cggtatcgat cacgagacta     2220

gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt catatttgca     2280

tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat     2340

attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa     2400

attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt     2460

ggctttatat atcttgtgga aaggacgaaa caccggagac gattaatgcg tctcggtttt     2520

agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc     2580

tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac     2640

cgagtcggtg ctttttttga attctcgacc tcgagacaaa tggcagtatt cgtcattagt     2700

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg     2760

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc     2820

aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc     2880

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg     2940

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat     3000

ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct     3060

ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg     3120

tgcagcgatg ggggcggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg     3180

gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa     3240

gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg     3300

ggcggggagt cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc     3360

cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct     3420

tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg     3480

cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt     3540

gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt     3600

gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg     3660

gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg     3720

ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc     3780

ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg     3840

gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc     3900

ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc     3960

gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag     4020

agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc     4080

cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc     4140

ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc     4200

tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg     4260

cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta     4320

cagctcctgg gcaacgtgct ggttattgtg gccaccatgg tgagcaaggg cgaggagctg     4380

ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc     4440

agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc     4500

tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc     4560

gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc     4620

atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag     4680

acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc     4740

atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc     4800

cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc     4860

cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc     4920

atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg     4980

agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc     5040

gggatcactc tcggcatgga cgagctgtac aagtaaagcg tctggaacaa tcaacctctg     5100

gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta     5160

tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt     5220

ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc     5280

aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt     5340

gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg     5400

gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac     5460

aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc     5520

acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac     5580

cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct     5640

cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag     5700

acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg     5760

cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt     5820

taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg     5880

gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc     5940

acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc     6000

cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg     6060

ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc     6120

cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc     6180

gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt     6240

ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca     6300

gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc     6360

tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg     6420

ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc     6480

ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt     6540

attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc     6600

tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc     6660

tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg     6720

taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc     6780

cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg     6840

gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc     6900

ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac     6960

agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa     7020

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     7080

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     7140

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     7200

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta     7260

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     7320

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     7380

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     7440

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg     7500

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     7560

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     7620

aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa     7680

cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat     7740

ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc     7800

tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc     7860

atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc     7920

tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc     7980

aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc     8040

catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt     8100

gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc     8160

ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa     8220

aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt     8280

atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg     8340

cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc     8400

gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa     8460

agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt     8520

gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt     8580

caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag     8640

ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta     8700

tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat     8760

aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca     8820

acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa     8880

ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt     8940

atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc     9000

cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct     9060

ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag     9120

t                                                                     9121


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Spacer sequence

<400> 6
ccttggtgaa gtctcctttg                                                   20


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Spacer sequence

<400> 7
atgcgggagg ctgagcgcac                                                   20


<210> 8
<211> 19
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<223> Ctb peptide sequence

<400> 8
Gly Gly Ser Gly Ser Ser Ser Thr Ser Thr Thr Ala Lys Arg Lys Lys 
1               5                   10                  15      


Arg Lys Leu 
            


<210> 9
<211> 240
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> sfGFP+15 peptide sequence

<400> 9
Gly Gly Ala Ser Lys Gly Glu Arg Leu Phe Thr Gly Val Val Pro Ile 
1               5                   10                  15      


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg 
            20                  25                  30          


Gly Glu Gly Glu Gly Asp Ala Thr Arg Gly Lys Leu Thr Leu Lys Phe 
        35                  40                  45              


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
    50                  55                  60                  


Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Lys His Met 
65                  70                  75                  80  


Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
                85                  90                  95      


Glu Arg Thr Ile Ser Phe Lys Lys Asp Gly Thr Tyr Lys Thr Arg Ala 
            100                 105                 110         


Glu Val Lys Phe Glu Gly Arg Thr Leu Val Asn Arg Ile Glu Leu Lys 
        115                 120                 125             


Gly Arg Asp Phe Lys Glu Lys Gly Asn Ile Leu Gly His Lys Leu Glu 
    130                 135                 140                 


Tyr Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Arg Lys 
145                 150                 155                 160 


Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Lys Asp Gly 
                165                 170                 175     


Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Arg 
            180                 185                 190         


Gly Pro Val Leu Leu Pro Arg Asn His Tyr Leu Ser Thr Arg Ser Ala 
        195                 200                 205             


Leu Ser Lys Asp Pro Lys Glu Lys Arg Asp His Met Val Leu Leu Glu 
    210                 215                 220                 


Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 240 


<210> 10
<211> 386
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> sssIM methyltransferase with methyltransferase point mutation

<400> 10
Met Ser Lys Val Glu Asn Lys Thr Lys Lys Leu Arg Val Phe Glu Ala 
1               5                   10                  15      


Phe Ala Gly Ile Gly Ala Gln Arg Lys Ala Leu Glu Lys Val Arg Lys 
            20                  25                  30          


Asp Glu Tyr Glu Ile Val Gly Leu Ala Glu Trp Tyr Val Pro Ala Ile 
        35                  40                  45              


Val Met Tyr Gln Ala Ile His Asn Asn Phe His Thr Lys Leu Glu Tyr 
    50                  55                  60                  


Lys Ser Val Ser Arg Glu Glu Met Ile Asp Tyr Leu Glu Asn Lys Thr 
65                  70                  75                  80  


Leu Ser Trp Asn Ser Lys Asn Pro Val Ser Asn Gly Tyr Trp Lys Arg 
                85                  90                  95      


Lys Lys Asp Asp Glu Leu Lys Ile Ile Tyr Asn Ala Ile Lys Leu Ser 
            100                 105                 110         


Glu Lys Glu Gly Asn Ile Phe Asp Ile Arg Asp Leu Tyr Lys Arg Thr 
        115                 120                 125             


Leu Lys Asn Ile Asp Leu Leu Thr Tyr Ser Phe Pro Cys Gln Asp Leu 
    130                 135                 140                 


Ser Gln Gln Gly Ile Gln Lys Gly Met Lys Arg Gly Ser Gly Thr Arg 
145                 150                 155                 160 


Ser Gly Leu Leu Trp Glu Ile Glu Arg Ala Leu Asp Ser Thr Glu Lys 
                165                 170                 175     


Asn Asp Leu Pro Lys Tyr Leu Leu Met Ala Asn Val Gly Ala Leu Leu 
            180                 185                 190         


His Lys Lys Asn Glu Glu Glu Leu Asn Gln Trp Lys Gln Lys Leu Glu 
        195                 200                 205             


Ser Leu Gly Tyr Gln Asn Ser Ile Glu Val Leu Asn Ala Ala Asp Phe 
    210                 215                 220                 


Gly Ser Ser Gln Ala Arg Arg Arg Val Phe Met Ile Ser Thr Leu Asn 
225                 230                 235                 240 


Glu Phe Val Glu Leu Pro Lys Gly Asp Lys Lys Pro Lys Ser Ile Lys 
                245                 250                 255     


Lys Val Leu Asn Lys Ile Val Ser Glu Lys Asp Ile Leu Asn Asn Leu 
            260                 265                 270         


Leu Lys Tyr Asn Leu Thr Glu Phe Lys Lys Thr Lys Ser Asn Ile Asn 
        275                 280                 285             


Lys Ala Ser Leu Ile Gly Tyr Ser Lys Phe Asn Ser Glu Gly Tyr Val 
    290                 295                 300                 


Tyr Asp Pro Glu Phe Thr Gly Pro Thr Leu Thr Ala Ser Gly Ala Asn 
305                 310                 315                 320 


Ser Arg Ile Lys Ile Lys Asp Gly Ser Asn Ile Arg Lys Met Asn Ser 
                325                 330                 335     


Asp Glu Thr Phe Leu Tyr Ile Gly Phe Asp Ser Gln Asp Gly Lys Arg 
            340                 345                 350         


Val Asn Glu Ile Glu Phe Leu Thr Glu Asn Gln Lys Ile Phe Val Cys 
        355                 360                 365             


Gly Asn Ser Ile Ser Val Glu Val Leu Glu Ala Ile Ile Asp Lys Ile 
    370                 375                 380                 


Gly Gly 
385     


<210> 11
<211> 64
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Point mutation sso7d

<400> 11
Met Ala Thr Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp 
1               5                   10                  15      


Ile Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe 
            20                  25                  30          


Thr Tyr Asp Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu 
        35                  40                  45              


Lys Asp Ala Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys 
    50                  55                  60                  


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 12
tagaggggcc gacggagatt                                                   20


<210> 13
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 13
agaaaggaag agtggggaa                                                    19


<210> 14
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 14
acacttccac tgtagtcag                                                    19


<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 15
taactccttg agtggggcgc                                                   20


<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 16
aagagagaca gtacatgccc                                                   20


<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 17
acgtggatga aggcgccgcg                                                   20


<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
ccaggaaaag gactttcaca                                                   20


<210> 19
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
gcgccgcagt aaagagagag g                                                 21


<210> 20
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
gtctaaccct aactgagaa                                                    19


<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 21
gcgcttgtgc ggagccggag g                                                 21


<210> 22
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 22
ctttccctac acgacgctct tccgatctnn nnnngcgggg actagaccag aagg             54


<210> 23
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 23
ctttccctac acgacgctct tccgatctnn nnnntcaggt ttggggctct tttg             54


<210> 24
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 24
ctttccctac acgacgctct tccgatctnn nnnngatccg gttagggagg ttgg             54


<210> 25
<211> 61
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 25
ctttccctac acgacgctct tccgatctnn nnnntctgta tccttaatgg tgttctctct       60

c                                                                       61


<210> 26
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 26
ctttccctac acgacgctct tccgatctnn nnnngcaccc agaatgaggt ggtc             54


<210> 27
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 27
ctttccctac acgacgctct tccgatctnn nnnntccaca ctctgaggcg gaac             54


<210> 28
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 28
ctttccctac acgacgctct tccgatctnn nnnnggagct ccagatggct aagg             54


<210> 29
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 29
ctttccctac acgacgctct tccgatctnn nnnnatcttt gagggcctgg gttg             54


<210> 30
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 30
ctttccctac acgacgctct tccgatctnn nnnncagtgg cccgctacaa gttc             54


<210> 31
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 31
ctttccctac acgacgctct tccgatctnn nnnntgttct ctggtgggca ggag             54


<210> 32
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 32
ctttccctac acgacgctct tccgatctnn nnnncggctg tggctactca gg               52


<210> 33
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 33
ctttccctac acgacgctct tccgatctnn nnnnagccgc gagagtcagc ttg              53


<210> 34
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer


<220>
<221> modified_base
<222> (29)..(34)
<223> a, c, t, g, unknown or other

<400> 34
ctttccctac acgacgctct tccgatctnn nnnnctgtgc tgagtcggaa gtgg             54


<210> 35
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 35
ggagttcaga cgtgtgctct tccgatctgg tctgtggatt cggtcctc                    48


<210> 36
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 36
ggagttcaga cgtgtgctct tccgatctgg aaagcatgat gggagagg                    48


<210> 37
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 37
ggagttcaga cgtgtgctct tccgatcttt gtcctgacac tggcatcc                    48


<210> 38
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 38
ggagttcaga cgtgtgctct tccgatcttc ctggcattga cattccac                    48


<210> 39
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 39
ggagttcaga cgtgtgctct tccgatctca tttcccaaat gcgctctc                    48


<210> 40
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 40
ggagttcaga cgtgtgctct tccgatctag gcccctgaaa gctgctac                    48


<210> 41
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 41
ggagttcaga cgtgtgctct tccgatctac cttccagcag gtctgtcg                    48


<210> 42
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 42
ggagttcaga cgtgtgctct tccgatctcc atgcacctcc gtaccttc                    48


<210> 43
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 43
ggagttcaga cgtgtgctct tccgatctgg ttgcggtcga ggaaaag                     47


<210> 44
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 44
ggagttcaga cgtgtgctct tccgatctta tgcccagatg gcctgaag                    48


<210> 45
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 45
ggagttcaga cgtgtgctct tccgatcttc tcccctctca cctctagcc                   49


<210> 46
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 46
ggagttcaga cgtgtgctct tccgatcttg ctctagaatg aacggtggaa g                51


<210> 47
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 47
ggagttcaga cgtgtgctct tccgatctcc aaatcagaca gggtcgaag                   49


