                         SEQUENCE LISTING

<110>  Cancer Research Technology and University of Birmingham
 
<120>  Antibodies and related molecules and uses thereof

<130>  16/0003/JA

<160>  119   

<170>  PatentIn version 3.5

<210>  1
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable heavy chain of CRT3 (SEQ ID NO.1)

<400>  1

Met Ala Glu Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro 
1               5                   10                  15      


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30          


Ser Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Ser Asp Thr Ser Tyr Asn Gln 
    50                  55                  60                  


Lys Phe Lys Gly Lys Ala Lys Leu Thr Ala Val Thr Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Phe 
                85                  90                  95      


Tyr Cys Thr His Tyr Tyr Gly Ser Asp Tyr Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Ser Val Thr Val 
        115                 


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of VH of CRT3 (SEQ ID NO. 2)

<400>  2

Gly Tyr Thr Phe Thr Ser Tyr Trp 
1               5               


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of VH of CRT3 (SEQ ID NO.3)

<400>  3

Ile Tyr Pro Gly Asn Ser Asp Thr 
1               5               


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of VH of CRT3 (SEQ ID NO. 4)

<400>  4

Thr His Tyr Tyr Gly Ser Asp Tyr Ala Met Asp Tyr 
1               5                   10          


<210>  5
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Varaible light chain of CRT3 (SEQ ID NO. 5)

<400>  5

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Met Thr Cys Thr Ala Ser Ser Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys Leu Trp 
        35                  40                  45              


Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu 
65                  70                  75                  80  


Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gln Tyr His Arg Ser Pro 
                85                  90                  95      


Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala 
            100                 105                 110         


<210>  6
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of VL of CRT3 (SEQ ID NO. 6)

<400>  6

Ser Ser Val Ser Ser Ser Tyr 
1               5           


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of VL of CRT3 (SEQ ID NO. 8)

<400>  7

His Gln Tyr His Arg Ser Pro Arg Thr 
1               5                   


<210>  8
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv of CRT3 (SEQ ID NO. 9)

<400>  8

Met Ala Glu Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro 
1               5                   10                  15      


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30          


Ser Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Ser Asp Thr Ser Tyr Asn Gln 
    50                  55                  60                  


Lys Phe Lys Gly Lys Ala Lys Leu Thr Ala Val Thr Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Phe 
                85                  90                  95      


Tyr Cys Thr His Tyr Tyr Gly Ser Asp Tyr Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr Gln Ser Pro 
    130                 135                 140                 


Ala Ile Met Ser Ala Ser Leu Gly Glu Arg Val Thr Met Thr Cys Thr 
145                 150                 155                 160 


Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Ser Ser Pro Lys Leu Trp Ile Tyr Ser Thr Ser Asn Leu Ala 
            180                 185                 190         


Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
        195                 200                 205             


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
    210                 215                 220                 


Cys His Gln Tyr His Arg Ser Pro Arg Thr Phe Gly Gly Gly Thr Lys 
225                 230                 235                 240 


Leu Glu Ile Lys Arg Ala Ala Ala 
                245             


<210>  9
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR3 for CRT3 (SEQ ID NO. 10)

<400>  9

Met Gly Val Leu Leu Thr Gln Arg Thr Leu Leu Ser Leu Val Leu Ala 
1               5                   10                  15      


Leu Leu Phe Pro Ser Met Ala Ser Met Ala Glu Val Gln Leu Gln Gln 
            20                  25                  30          


Ser Gly Thr Val Leu Ala Arg Pro Gly Ala Ser Val Lys Met Ser Cys 
        35                  40                  45              


Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Trp Met His Trp Val Lys 
    50                  55                  60                  


Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Ala Ile Tyr Pro Gly 
65                  70                  75                  80  


Asn Ser Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly Lys Ala Lys Leu 
                85                  90                  95      


Thr Ala Val Thr Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu 
            100                 105                 110         


Thr Asn Glu Asp Ser Ala Val Phe Tyr Cys Thr His Tyr Tyr Gly Ser 
        115                 120                 125             


Asp Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Leu Gly 
                165                 170                 175     


Glu Arg Val Thr Met Thr Cys Thr Ala Ser Ser Ser Val Ser Ser Ser 
            180                 185                 190         


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys Leu Trp 
        195                 200                 205             


Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu 
225                 230                 235                 240 


Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gln Tyr His Arg Ser Pro 
                245                 250                 255     


Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala 
            260                 265                 270         


Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
        275                 280                 285             


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
    290                 295                 300                 


Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
305                 310                 315                 320 


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                325                 330                 335     


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
            340                 345                 350         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        355                 360                 365             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser 
    370                 375                 380                 


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
385                 390                 395                 400 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                405                 410                 415     


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
            420                 425                 430         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        435                 440                 445             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    450                 455                 460                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
465                 470                 475                 480 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490         


<210>  10
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence for variable heavy chian of CRT3 (SEQ ID NO. 
       11)

<400>  10
atggccgagg tccagctgca gcagtctggg actgtgctgg caaggcctgg ggcttcagtg       60

aagatgtcct gcaaggcttc tggctacacc tttaccagct actggatgca ctgggtaaaa      120

cagaggcctg gacagggtct ggaatggatt ggcgctattt atcctggaaa tagtgatact      180

agctacaacc agaagttcaa gggcaaggcc aaactgactg cagtcacatc caccagcact      240

gcctacatgg agctcagcag cctgacaaat gaggactctg cggtctttta ctgtacacat      300

tactacggta gtgactatgc tatggactac tggggtcaag gaacctcagt cactgtc         357


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence for CDR1 of VH of CRT3 (SEQ ID NO. 12)

<400>  11
ggctacacct ttaccagcta ctgg                                              24


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR2 of VH of CRT3 (SEQ ID NO. 13)

<400>  12
atttatcctg gaaatagtga tact                                              24


<210>  13
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence for CDR3 of VH of CRT3 (SEQ ID NO. 14)

<400>  13
acacattact acggtagtga ctatgctatg gactac                                 36


<210>  14
<211>  327
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of variable light chain of CRT3 (SEQ ID NO. 
       15)

<400>  14
caaattgttc tcacccagtc tccagcaatc atgtctgcat ctctagggga acgggtcacc       60

atgacctgca ctgccagctc aagtgtaagt tccagttact tgcactggta ccagcagaag      120

ccaggatcct cccccaaact ctggatttat agcacatcca acctggcttc tggagtccca      180

gctcgcttca gtggcagtgg gtctgggacc tcttactctc tcacaatcag cagcatggag      240

gctgaagatg ctgccactta ttactgccac cagtatcatc gttccccacg gacgttcggt      300

ggaggcacca agctggaaat caaacgt                                          327


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR1 of VL of CRT3 (SEQ ID NO. 16)

<400>  15
tcaagtgtaa gttccagtta c                                                 21


<210>  16
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of CDR3 of VL of CRT3 (SEQ ID NO. 18)

<400>  16
caccagtatc atcgttcccc acggacg                                           27


<210>  17
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of scFv for CRT3 (SEQ ID NO. 19)

<400>  17
atggccgagg tccagctgca gcagtctggg actgtgctgg caaggcctgg ggcttcagtg       60

aagatgtcct gcaaggcttc tggctacacc tttaccagct actggatgca ctgggtaaaa      120

cagaggcctg gacagggtct ggaatggatt ggcgctattt atcctggaaa tagtgatact      180

agctacaacc agaagttcaa gggcaaggcc aaactgactg cagtcacatc caccagcact      240

gcctacatgg agctcagcag cctgacaaat gaggactctg cggtctttta ctgtacacat      300

tactacggta gtgactatgc tatggactac tggggtcaag gaacctcagt cactgtctcc      360

tcaggtggag gcggttcagg cggaggtggc tctggcggtg gcggatcgca aattgttctc      420

acccagtctc cagcaatcat gtctgcatct ctaggggaac gggtcaccat gacctgcact      480

gccagctcaa gtgtaagttc cagttacttg cactggtacc agcagaagcc aggatcctcc      540

cccaaactct ggatttatag cacatccaac ctggcttctg gagtcccagc tcgcttcagt      600

ggcagtgggt ctgggacctc ttactctctc acaatcagca gcatggaggc tgaagatgct      660

gccacttatt actgccacca gtatcatcgt tccccacgga cgttcggtgg aggcaccaag      720

ctggaaatca aacgtgcggc cgca                                             744


<210>  18
<211>  1499
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CAR3 for CRT3 (SEQ ID NO. 20)

<400>  18
atgggcgtgc tgctgaccca gaggaccctg ctgagcctgg tgctggccct gctgtttcca       60

tctatggcat cgatggccga ggtccagctg cagcagtctg ggactgtgct ggcaaggcct      120

ggggcttcag tgaagatgtc ctgcaaggct tctggctaca cctttaccag ctactggatg      180

cactgggtaa aacagaggcc tggacagggt ctggaatgga ttggcgctat ttatcctgga      240

aatagtgata ctagctacaa ccagaagttc aagggcaagg ccaaactgac tgcagtcaca      300

tccaccagca ctgcctacat ggagctcagc agcctgacaa atgaggactc tgcggtcttt      360

tactgtacac attactacgg tagtgactat gctatggact actggggtca aggaacctca      420

gtcactgtct cctcaggtgg aggcggttca ggcggaggtg gctctggcgg tggcggatcg      480

caaattgttc tcacccagtc tccagcaatc atgtctgcat ctctagggga acgggtcacc      540

atgacctgca ctgccagctc aagtgtaagt tccagttact tgcactggta ccagcagaag      600

ccaggatcct cccccaaact ctggatttat agcacatcca acctggcttc tggagtccca      660

gctcgcttca gtggcagtgg gtctgggacc tcttactctc tcacaatcag cagcatggag      720

gctgaagatg ctgccactta ttactgccac cagtatcatc gttccccacg gacgttcggt      780

ggaggcacca agctggaaat caaacgtgcg gccgcaattg aagttatgta tcctcctcct      840

tacctagaca atgagaagag caatggaacc attatccatg tgaaagggaa acacctttgt      900

ccaagtcccc tatttcccgg accttctaag cccttttggg tgctggtggt ggttggtgga      960

gtcctggctt gctatagctt gctagtaaca gtggccttta ttattttctg ggtgaggagt     1020

aagaggagca ggctcctgca cagtgactac atgaacatga ctccccgccg ccccgggccc     1080

acccgcaagc attaccagcc ctatgcccca ccacgcgact tcgcagccta tcgctccaga     1140

gtgaagttca gcaggagcgc agacgccccc gcgtaccagc agggccagaa ccagctctat     1200

aacgagctca atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg     1260

gaccctgaga tggggggaaa gccgcagaga aggaagaacc ctcaggaagg cctgtacaat     1320

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc     1380

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc     1440

tacgacgccc ttcacatgca ggccctgccc cctcgctaat aaaagcttaa cacgagcca      1499


<210>  19
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable light chain for CRT2 (SEQ ID NO. 25)

<400>  19

Glu Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Phe Gln Gln Lys Pro Gly Thr Ser Pro Lys Leu Trp Ile Tyr 
        35                  40                  45              


Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Arg Ser Ser Tyr Pro Leu Thr 
                85                  90                  95      


Phe Gly Ala Pro Gly Lys Leu Glu Leu Lys Arg Ala Ala 
            100                 105                 


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of VL of CRT2 (SEQ ID NO. 26)

<400>  20

Ser Tyr Met His Trp Phe 
1               5       


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of VL of CRT2 (SEQ ID NO. 27)

<400>  21

Leu Trp Ile Tyr Ser Thr Ser Asn Leu Ala 
1               5                   10  


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of VL of CRT2 (SEQ ID NO. 28)

<400>  22

Gln Gln Arg Ser Ser Tyr Pro Leu 
1               5               


<210>  23
<211>  329
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence for Variable light chain of CRT2 (SEQ ID NO. 
       35)

<400>  23
gaaattgttc tcacccagtc tccagcaatc atgtctgcat ctccagggga gaaggtcacc       60

ataacctgca gtgccagctc aagtgtaagt tacatgcact ggttccagca gaagccaggc      120

acttctccca aactctggat ttatagcaca tccaacctgg cttctggagt ccctgctcgc      180

ttcagtggca gtggatctgg gacctcttac tctctcacaa tcagccgaat ggaggctgaa      240

gatgctgcca cttattactg ccagcaaagg agtagttacc ccctcacgtt cggtgctggg      300

accaagctgg agctgaaacg tgcggccgc                                        329


<210>  24
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR1 of VL of CRT2 (SEQ ID NO. 36)

<400>  24
agttacatgc actggttc                                                     18


<210>  25
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR2 of VL of CRT2 (SEQ ID NO. 37)

<400>  25
ctctggattt atagcacatc caacctggct                                        30


<210>  26
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence for CDR3 of VL of CRT2 (SEQ ID NO. 38)

<400>  26
cagcaaagga gtagttaccc cctc                                              24


<210>  27
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable heavy chain of CRT3 variant (SEQ ID NO. 41)

<400>  27

Met Ala Glu Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro 
1               5                   10                  15      


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30          


Ser Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Ser Asp Thr Ser Tyr Asn Gln 
    50                  55                  60                  


Lys Phe Lys Gly Lys Ala Lys Leu Thr Ala Val Thr Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Phe 
                85                  90                  95      


Tyr Cys Thr His Tyr Gly Ser Asp Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Ile Ser Ser Gly 
        115                 120     


<210>  28
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of VH of CRT3 variant (SEQ ID NO. 42)

<400>  28

Thr Ser Tyr Trp Met His 
1               5       


<210>  29
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of VH of CRT3 variant (SEQ ID NO. 43)

<400>  29

Trp Ile Gly Ala Ile Tyr Pro Gly Asn Ser Asp Thr Ser 
1               5                   10              


<210>  30
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of VH of CRT3 variant (SEQ ID NO. 44)

<400>  30

Thr His Tyr Tyr Gly Ser Asp Tyr Ala Met Asp 
1               5                   10      


<210>  31
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable light chain of CRT3 variant (SEQ ID NO. 45)

<400>  31

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Met Thr Cys Thr Ala Ser Ser Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys Leu Trp 
        35                  40                  45              


Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu 
65                  70                  75                  80  


Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gln Tyr His Arg Ser Pro 
                85                  90                  95      


Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala 
            100                 105                 110     


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of VL of CRT3 variant (SEQ ID NO. 46)

<400>  32

Ser Ser Ser Tyr Leu His Trp Tyr 
1               5               


<210>  33
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of VL of CRT3 variant (SEQ ID NO. 47)

<400>  33

Leu Trp Ile Tyr Ser Thr Ser Asn Leu Ala 
1               5                   10  


<210>  34
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of VL of CRT3 variant (SEQ ID NO. 48)

<400>  34

His Gln Tyr His Arg Ser Pro Arg 
1               5               


<210>  35
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv comprising VH of variant CRT3 and VL of non-variant CRT3 
       (SEQ ID NO. 49)

<400>  35

Met Ala Glu Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro 
1               5                   10                  15      


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30          


Ser Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Ser Asp Thr Ser Tyr Asn Gln 
    50                  55                  60                  


Lys Phe Lys Gly Lys Ala Lys Leu Thr Ala Val Thr Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Phe 
                85                  90                  95      


Tyr Cys Thr His Tyr Gly Ser Asp Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Ile Ser Ser Gly Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr Gln 
    130                 135                 140                 


Ser Pro Ala Ile Met Ser Ala Ser Leu Gly Glu Arg Val Thr Met Thr 
145                 150                 155                 160 


Cys Thr Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln 
                165                 170                 175     


Gln Lys Pro Gly Ser Ser Pro Lys Leu Trp Ile Tyr Ser Thr Ser Asn 
            180                 185                 190         


Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
    210                 215                 220                 


Tyr Tyr Cys His Gln Tyr His Arg Ser Pro Arg Thr Phe Gly Gly Gly 
225                 230                 235                 240 


Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala 
                245                 250 


<210>  36
<211>  247
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv comprising VH of CRT3 and VL of CRT3 variant (SEQ ID NO. 50)

<400>  36

Met Ala Glu Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro 
1               5                   10                  15      


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30          


Ser Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Ser Asp Thr Ser Tyr Asn Gln 
    50                  55                  60                  


Lys Phe Lys Gly Lys Ala Lys Leu Thr Ala Val Thr Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Phe 
                85                  90                  95      


Tyr Cys Thr His Tyr Tyr Gly Ser Asp Tyr Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr Gln Ser Pro 
    130                 135                 140                 


Ala Ile Met Ser Ala Ser Leu Gly Glu Arg Val Thr Met Thr Cys Thr 
145                 150                 155                 160 


Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Ser Ser Pro Lys Leu Trp Ile Tyr Ser Thr Ser Asn Leu Ala 
            180                 185                 190         


Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr 
        195                 200                 205             


Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr 
    210                 215                 220                 


Cys His Gln Tyr His Arg Ser Pro Arg Thr Phe Gly Gly Gly Thr Lys 
225                 230                 235                 240 


Leu Glu Ile Lys Arg Ala Ala 
                245         


<210>  37
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv comprising VH and VL of CRT3 variant (SEQ ID NO. 51)

<400>  37

Met Ala Glu Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro 
1               5                   10                  15      


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30          


Ser Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Ser Asp Thr Ser Tyr Asn Gln 
    50                  55                  60                  


Lys Phe Lys Gly Lys Ala Lys Leu Thr Ala Val Thr Ser Thr Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Phe 
                85                  90                  95      


Tyr Cys Thr His Tyr Gly Ser Asp Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Ile Ser Ser Gly Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ile Val Leu Thr Gln 
    130                 135                 140                 


Ser Pro Ala Ile Met Ser Ala Ser Leu Gly Glu Arg Val Thr Met Thr 
145                 150                 155                 160 


Cys Thr Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln 
                165                 170                 175     


Gln Lys Pro Gly Ser Ser Pro Lys Leu Trp Ile Tyr Ser Thr Ser Asn 
            180                 185                 190         


Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
    210                 215                 220                 


Tyr Tyr Cys His Gln Tyr His Arg Ser Pro Arg Thr Phe Gly Gly Gly 
225                 230                 235                 240 


Thr Lys Leu Glu Ile Lys Arg Ala Ala 
                245                 


<210>  38
<211>  490
<212>  PRT
<213>  Homo sapiens

<400>  38

Met Arg Pro Ala Phe Ala Leu Cys Leu Leu Trp Gln Ala Leu Trp Pro 
1               5                   10                  15      


Gly Pro Gly Gly Gly Glu His Pro Thr Ala Asp Arg Ala Gly Cys Ser 
            20                  25                  30          


Ala Ser Gly Ala Cys Tyr Ser Leu His His Ala Thr Met Lys Arg Gln 
        35                  40                  45              


Ala Ala Glu Glu Ala Cys Ile Leu Arg Gly Gly Ala Leu Ser Thr Val 
    50                  55                  60                  


Arg Ala Gly Ala Glu Leu Arg Ala Val Leu Ala Leu Leu Arg Ala Gly 
65                  70                  75                  80  


Pro Gly Pro Gly Gly Gly Ser Lys Asp Leu Leu Phe Trp Val Ala Leu 
                85                  90                  95      


Glu Arg Arg Arg Ser His Cys Thr Leu Glu Asn Glu Pro Leu Arg Gly 
            100                 105                 110         


Phe Ser Trp Leu Ser Ser Asp Pro Gly Gly Leu Glu Ser Asp Thr Leu 
        115                 120                 125             


Gln Trp Val Glu Glu Pro Gln Arg Ser Cys Thr Ala Arg Arg Cys Ala 
    130                 135                 140                 


Val Leu Gln Ala Thr Gly Gly Val Glu Pro Ala Gly Trp Lys Glu Met 
145                 150                 155                 160 


Arg Cys His Leu Arg Ala Asn Gly Tyr Leu Cys Lys Tyr Gln Phe Glu 
                165                 170                 175     


Val Leu Cys Pro Ala Pro Arg Pro Gly Ala Ala Ser Asn Leu Ser Tyr 
            180                 185                 190         


Arg Ala Pro Phe Gln Leu His Ser Ala Ala Leu Asp Phe Ser Pro Pro 
        195                 200                 205             


Gly Thr Glu Val Ser Ala Leu Cys Arg Gly Gln Leu Pro Ile Ser Val 
    210                 215                 220                 


Thr Cys Ile Ala Asp Glu Ile Gly Ala Arg Trp Asp Lys Leu Ser Gly 
225                 230                 235                 240 


Asp Val Leu Cys Pro Cys Pro Gly Arg Tyr Leu Arg Ala Gly Lys Cys 
                245                 250                 255     


Ala Glu Leu Pro Asn Cys Leu Asp Asp Leu Gly Gly Phe Ala Cys Glu 
            260                 265                 270         


Cys Ala Thr Gly Phe Glu Leu Gly Lys Asp Gly Arg Ser Cys Val Thr 
        275                 280                 285             


Ser Gly Glu Gly Gln Pro Thr Leu Gly Gly Thr Gly Val Pro Thr Arg 
    290                 295                 300                 


Arg Pro Pro Ala Thr Ala Thr Ser Pro Val Pro Gln Arg Thr Trp Pro 
305                 310                 315                 320 


Ile Arg Val Asp Glu Lys Leu Gly Glu Thr Pro Leu Val Pro Glu Gln 
                325                 330                 335     


Asp Asn Ser Val Thr Ser Ile Pro Glu Ile Pro Arg Trp Gly Ser Gln 
            340                 345                 350         


Ser Thr Met Ser Thr Leu Gln Met Ser Leu Gln Ala Glu Ser Lys Ala 
        355                 360                 365             


Thr Ile Thr Pro Ser Gly Ser Val Ile Ser Lys Phe Asn Ser Thr Thr 
    370                 375                 380                 


Ser Ser Ala Thr Pro Gln Ala Phe Asp Ser Ser Ser Ala Val Val Phe 
385                 390                 395                 400 


Ile Phe Val Ser Thr Ala Val Val Val Leu Val Ile Leu Thr Met Thr 
                405                 410                 415     


Val Leu Gly Leu Val Lys Leu Cys Phe His Glu Ser Pro Ser Ser Gln 
            420                 425                 430         


Pro Arg Lys Glu Ser Met Gly Pro Pro Gly Leu Glu Ser Asp Pro Glu 
        435                 440                 445             


Pro Ala Ala Leu Gly Ser Ser Ser Ala His Cys Thr Asn Asn Gly Val 
    450                 455                 460                 


Lys Val Gly Asp Cys Asp Leu Arg Asp Arg Ala Glu Gly Ala Leu Leu 
465                 470                 475                 480 


Ala Glu Ser Pro Leu Gly Ser Ser Asp Ala 
                485                 490 


<210>  39
<211>  1380
<212>  DNA
<213>  Homo sapiens

<400>  39
ctcctcttgc tctaagcagg gtgtttgacc ttctagtcga ctgcgtcccc tgtacccggc       60

gccagctgtg ttcctgaccc cagaataact cagggctgca ccgggcctgg cagcgctccg      120

cacacatttc ctgtcgcggc ctaagggaaa ctgttggccg ctgggcccgc ggggggattc      180

ttggcagttg gggggtccgt cgggagcgag ggcggagggg aagggagggg gaaccgggtt      240

ggggaagcca gctgtagagg gcggtgaccg cgctccagac acagctctgc gtcctcgagc      300

gggacagatc caagttggga gcagctctgc gtgcggggcc tcagagaatg aggccggcgt      360

tcgccctgtg cctcctctgg caggcgctct ggcccgggcc gggcggcggc gaacacccca      420

ctgccgaccg tgctggctgc tcggcctcgg gggcctgcta cagcctgcac cacgctacca      480

tgaagcggca ggcggccgag gaggcctgca tcctgcgagg tggggcgctc agcaccgtgc      540

gtgcgggcgc cgagctgcgc gctgtgctcg cgctcctgcg ggcaggccca gggcccggag      600

ggggctccaa agacctgctg ttctgggtcg cactggagcg caggcgttcc cactgcaccc      660

tggagaacga gcctttgcgg ggtttctcct ggctgtcctc cgaccccggc ggtctcgaaa      720

gcgacacgct gcagtgggtg gaggagcccc aacgctcctg caccgcgcgg agatgcgcgg      780

tactccaggc caccggtggg gtcgagcccg caggctggaa ggagatgcga tgccacctgc      840

gcgccaacgg ctacctgtgc aagtaccagt ttgaggtctt gtgtcctgcg ccgcgccccg      900

gggccgcctc taacttgagc tatcgcgcgc ccttccagct gcacagcgcc gctctggact      960

tcagtccacc tgggaccgag gtgagtgcgc tctgccgggg acagctcccg atctcagtta     1020

cttgcatcgc ggacgaaatc ggcgctcgct gggacaaact ctcgggcgat gtgttgtgtc     1080

cctgccccgg gaggtacctc cgtgctggca aatgcgcaga gctccctaac tgcctagacg     1140

acttgggagg ctttgcctgc gaatgtgcta cgggcttcga gctggggaag gacggccgct     1200

cttgtgtgac cagtggggaa ggacagccga cccttggggg gaccggggtg cccaccaggc     1260

gcccgccggc cactgcaacc agccccgtgc cgcagagaac atggccaatc agggtcgacg     1320

agaagctggg agagacacca cttgtccctg aacaagacaa ttcagtaaca tctattcctg     1380


<210>  40
<211>  363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of variable heavy chain of CRT3 variant (SEQ 
       ID NO. 54)

<400>  40
atggccgagg tccagctgca gcagtctggg actgtgctgg caaggcctgg ggcttcagtg       60

aagatgtcct gcaaggcttc tggctacacc tttaccagct actggatgca ctgggtaaaa      120

cagaggcctg gacagggtct ggaatggatt ggcgctattt atcctggaaa tagtgatact      180

agctacaacc agaagttcaa gggcaaggcc aaactgactg cagtcacatc caccagcact      240

gcctacatgg agctcagcag cctgacaaat gaggactctg cggtctttta ctgtacacat      300

tactacggta gtgactatgc tatggactac tggggtcaag gaacctcagt cactgtctcc      360

tca                                                                    363


<210>  41
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR1 VH of CRT3 variant (SEQ ID NO. 55)

<400>  41
accagctact ggatgcac                                                     18


<210>  42
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR2 of VH of CRT3 variant (SEQ ID NO. 56)

<400>  42
tggattggcg ctatttatcc tggaaatagt gatactagc                              39


<210>  43
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR3 of VH of CRT3 variant (SEQ ID NO. 57)

<400>  43
acacattact acggtagtga ctatgctatg gac                                    33


<210>  44
<211>  335
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VL of CRT3 variant (SEQ ID NO. 58)

<400>  44
caaattgttc tcacccagtc tccagcaatc atgtctgcat ctctagggga acgggtcacc       60

atgacctgca ctgccagctc aagtgtaagt tccagttact tgcactggta ccagcagaag      120

ccaggatcct cccccaaact ctggatttat agcacatcca acctggcttc tggagtccca      180

gctcgcttca gtggcagtgg gtctgggacc tcttactctc tcacaatcag cagcatggag      240

gctgaagatg ctgccactta ttactgccac cagtatcatc gttccccacg gacgttcggt      300

ggaggcacca agctggaaat caaacgtgcg gccgc                                 335


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR1 of VL of CRT3 variant (SEQ ID NO. 59)

<400>  45
agttccagtt acttgcactg gtac                                              24


<210>  46
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR2 of VL of CRT3 variant (SEQ ID NO. 60)

<400>  46
ctctggattt atagcacatc caacctggct                                        30


<210>  47
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of CDR3 of VL of CRt3 variant (SEQ ID NO. 61)

<400>  47
ccaccagtat catcgttccc cacgg                                             25


<210>  48
<211>  741
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence for scFv comprising VH of CRT3 variant and VL
       of CRT3 (SEQ ID NO. 62)

<400>  48
atggccgagg tccagctgca gcagtctggg actgtgctgg caaggcctgg ggcttcagtg       60

aagatgtcct gcaaggcttc tggctacacc tttaccagct actggatgca ctgggtaaaa      120

cagaggcctg gacagggtct ggaatggatt ggcgctattt atcctggaaa tagtgatact      180

agctacaacc agaagttcaa gggcaaggcc aaactgactg cagtcacatc caccagcact      240

gcctacatgg agctcagcag cctgacaaat gaggactctg cggtctttta ctgtacacat      300

tactacggta gtgactatgc tatggactac tggggtcaag gaacctcagt cactgtctcc      360

tcatcctcag gtggaggcgg ttcaggcgga ggtggctctg gcggtggcgg atcgcaaatt      420

gttctcaccc agtctccagc aatcatgtct gcatctctag gggaacgggt caccatgacc      480

tgcactgcca gctcaagtgt aagttccagt tacttgcact ggtaccagca gaagccagga      540

tcctccccca aactctggat ttatagcaca tccaacctgg cttctggagt cccagctcgc      600

ttcagtggca gtgggtctgg gacctcttac tctctcacaa tcagcagcat ggaggctgaa      660

gatgctgcca cttattactg ccaccagtat catcgttccc cacggacgtt cggtggaggc      720

accaagctgg aaatcaaacg t                                                741


<210>  49
<211>  743
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence for scFv comrprising VH of CRT3 and VL of 
       CRT3 variant (SEQ ID NO. 63)

<400>  49
atggccgagg tccagctgca gcagtctggg actgtgctgg caaggcctgg ggcttcagtg       60

aagatgtcct gcaaggcttc tggctacacc tttaccagct actggatgca ctgggtaaaa      120

cagaggcctg gacagggtct ggaatggatt ggcgctattt atcctggaaa tagtgatact      180

agctacaacc agaagttcaa gggcaaggcc aaactgactg cagtcacatc caccagcact      240

gcctacatgg agctcagcag cctgacaaat gaggactctg cggtctttta ctgtacacat      300

tactacggta gtgactatgc tatggactac tggggtcaag gaacctcagt cactgtctcc      360

tcaggtggag gcggttcagg cggaggtggc tctggcggtg gcggatcgca aattgttctc      420

acccagtctc cagcaatcat gtctgcatct ctaggggaac gggtcaccat gacctgcact      480

gccagctcaa gtgtaagttc cagttacttg cactggtacc agcagaagcc aggatcctcc      540

cccaaactct ggatttatag cacatccaac ctggcttctg gagtcccagc tcgcttcagt      600

ggcagtgggt ctgggacctc ttactctctc acaatcagca gcatggaggc tgaagatgct      660

gccacttatt actgccacca gtatcatcgt tccccacgga cgttcggtgg aggcaccaag      720

ctggaaatca aacgtgcggc cgc                                              743


<210>  50
<211>  749
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of scFv comrprising VH of CRT3 variant and VL
       of CRT3 variant (SEQ ID NO. 64)

<400>  50
atggccgagg tccagctgca gcagtctggg actgtgctgg caaggcctgg ggcttcagtg       60

aagatgtcct gcaaggcttc tggctacacc tttaccagct actggatgca ctgggtaaaa      120

cagaggcctg gacagggtct ggaatggatt ggcgctattt atcctggaaa tagtgatact      180

agctacaacc agaagttcaa gggcaaggcc aaactgactg cagtcacatc caccagcact      240

gcctacatgg agctcagcag cctgacaaat gaggactctg cggtctttta ctgtacacat      300

tactacggta gtgactatgc tatggactac tggggtcaag gaacctcagt cactgtctcc      360

tcatcctcag gtggaggcgg ttcaggcgga ggtggctctg gcggtggcgg atcgcaaatt      420

gttctcaccc agtctccagc aatcatgtct gcatctctag gggaacgggt caccatgacc      480

tgcactgcca gctcaagtgt aagttccagt tacttgcact ggtaccagca gaagccagga      540

tcctccccca aactctggat ttatagcaca tccaacctgg cttctggagt cccagctcgc      600

ttcagtggca gtgggtctgg gacctcttac tctctcacaa tcagcagcat ggaggctgaa      660

gatgctgcca cttattactg ccaccagtat catcgttccc cacggacgtt cggtggaggc      720

accaagctgg aaatcaaacg tgcggccgc                                        749


<210>  51
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (G4S)2 linker (SEQ ID NO. 65)

<400>  51

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  52
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge domain of CD8alpha (SEQ ID NO. 66)

<400>  52

Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
1               5                   10                  15      


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            20                  25                  30          


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        35                  40                  45              


Gly Leu Asp Phe Ala Cys Asp 
    50                  55  


<210>  53
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Shortened IgG hinge (SEQ ID NO. 67)

<400>  53

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro 
1               5                   10              


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker sequence (SEQ ID NO. 68)

<400>  54

Lys Asp Pro Lys 
1               


<210>  55
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Shortened IgG hinge and linker (SEQ ID NO. 69)

<400>  55

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Lys Asp Pro 
1               5                   10                  15      


Lys 
    


<210>  56
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8alpha transmembrane domain (SEQ ID NO. 70)

<400>  56

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
            20                  25              


<210>  57
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain (SEQ ID NO. 71)

<400>  57

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  58
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CH2CH3 hinge (SEQ ID NO. 72)

<400>  58

Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro 
1               5                   10                  15      


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
            20                  25                  30          


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
        35                  40                  45              


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
    50                  55                  60                  


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
65                  70                  75                  80  


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
                85                  90                  95      


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
            100                 105                 110         


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
        115                 120                 125             


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
    130                 135                 140                 


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
145                 150                 155                 160 


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
                165                 170                 175     


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
            180                 185                 190         


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
        195                 200                 205             


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
    210                 215                 220                 


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys 
225                 230                 235                 


<210>  59
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta domain (SEQ ID NO. 73)

<400>  59

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  60
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellular domain of 41BB (SEQ ID NO. 74)

<400>  60

Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
1               5                   10                  15      


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
            20                  25                  30          


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40                  45          


<210>  61
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intracellualr domain of human CD28 (SEQ ID NO. 75)

<400>  61

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  62
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OX40 costimulatory domain (SEQ ID NO. 76)

<400>  62

Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly 
1               5                   10                  15      


Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr 
            20                  25                  30          


Leu Ala Lys Ile 
        35      


<210>  63
<211>  1101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of truncated CD34 (SEQ ID NO. 77)

<400>  63
atgcctcgcg gctggacagc cctgtgcctg ctgtctctgc tgccatccgg cttcatgagc       60

ctggataata acggcacagc caccccagag ctgcctacac agggcacctt cagcaatgtg      120

tccacaaacg tgagctatca ggagaccaca accccttcta ccctgggatc cacaagcctg      180

caccccgtgt ctcagcacgg caacgaagcc accaccaaca tcaccgagac cacagtgaag      240

tttacctcca cctctgtgat tacctctgtg tacggaaata caaactccag cgtgcagtct      300

cagacatctg tgatctccac agtgtttaca acacctgcca atgtgtccac cccagagaca      360

accctgaagc ccagcctgtc tcctggaaat gtgtccgatc tgtctaccac ctccaccagc      420

ctggccacct ctcccaccaa gccctatacc tcctcttctc ccatcctgag cgatatcaaa      480

gccgagatca aatgcagcgg gattcgggaa gtgaaactga cacagggcat ctgcctggaa      540

cagaataaga catccagctg cgccgagttt aagaaagata gaggagaggg actggccagg      600

gtgctgtgtg gcgaagagca ggccgacgcc gatgccggcg cccaggtgtg ttccctgctg      660

ctggcccagt ctgaggtgcg cccccagtgc ctgctgctgg tgctggccaa tcggacagaa      720

attagcagca agctgcagct gatgaaaaaa caccagagcg atctgaaaaa gctgggcatc      780

ctggacttta ccgagcagga cgtggcctct caccagagct acagccagaa aacactgatc      840

gccctggtga ccagcggagc cctgctggcc gtgctgggca tcaccggata tttcctgatg      900

aataggcgca gctggagccc caccggcgag cggctggagc tggagcctgt cgaccgagtg      960

aagcagaccc tgaactttga tctgctgaag ctggccggcg acgtggagtc caaccccggg     1020

ccagggaata tgggcgtgct gctgacccag aggaccctgc tgagcctggt gctggccctg     1080

ctgtttccat ctatggcatc g                                               1101


<210>  64
<211>  367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated CD34 (SEQ ID NO. 78)

<400>  64

Met Pro Arg Gly Trp Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser 
1               5                   10                  15      


Gly Phe Met Ser Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro 
            20                  25                  30          


Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu 
        35                  40                  45              


Thr Thr Thr Pro Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser 
    50                  55                  60                  


Gln His Gly Asn Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys 
65                  70                  75                  80  


Phe Thr Ser Thr Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser 
                85                  90                  95      


Ser Val Gln Ser Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro 
            100                 105                 110         


Ala Asn Val Ser Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro 
        115                 120                 125             


Gly Asn Val Ser Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser 
    130                 135                 140                 


Pro Thr Lys Pro Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys 
145                 150                 155                 160 


Ala Glu Ile Lys Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly 
                165                 170                 175     


Ile Cys Leu Glu Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys 
            180                 185                 190         


Asp Arg Gly Glu Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala 
        195                 200                 205             


Asp Ala Asp Ala Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser 
    210                 215                 220                 


Glu Val Arg Pro Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu 
225                 230                 235                 240 


Ile Ser Ser Lys Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys 
                245                 250                 255     


Lys Leu Gly Ile Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln 
            260                 265                 270         


Ser Tyr Ser Gln Lys Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu 
        275                 280                 285             


Leu Ala Val Leu Gly Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser 
    290                 295                 300                 


Trp Ser Pro Thr Gly Glu Arg Leu Glu Leu Glu Pro Val Asp Arg Val 
305                 310                 315                 320 


Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu 
                325                 330                 335     


Ser Asn Pro Gly Pro Gly Asn Met Gly Val Leu Leu Thr Gln Arg Thr 
            340                 345                 350         


Leu Leu Ser Leu Val Leu Ala Leu Leu Phe Pro Ser Met Ala Ser 
        355                 360                 365         


<210>  65
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CLEC14A primer (SEQ ID NO. 79)

<400>  65
ctgggaccga ggtgagtg                                                     18


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CLEC14A probe (SEQ ID NO. 80)

<400>  66
cgcgatgcaa gtaactgaga                                                   20


<210>  67
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Flotillin 2 primer (SEQ ID NO. 81)

<400>  67
tgttgtggtt ccgactataa acag                                              24


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Flotillin 2 probe (SEQ ID NO. 82)

<400>  68
gggctgcaac gtcataatct                                                   20


<210>  69
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CLEC14A fwd primer (SEQ ID NO. 83)

<400>  69
tagtaggaat tcgagagaat gaggccggcg ttcgccctg                              39


<210>  70
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CLEC14A rev primer (SEQ ID NO. 84)

<400>  70
agaaccgcgg ccgctggagg agtcgaaagc ctgaggagt                              39


<210>  71
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CLEC14A primer (SEQ ID NO. 85)

<400>  71
tagtaggaat tcgagagaat gaggccagcg cttgccctg                              39


<210>  72
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CLEC14A primer (SEQ ID NO. 86)

<400>  72
ctactagcgg ccgctcgtgg aagaggtgtc gaaagt                                 36


<210>  73
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human CLEC14A fwd primer (SEQ ID NO. 87)

<400>  73
tagtagttaa ttaagagaga atgaggccgg cgttc                                  35


<210>  74
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CLEC14A fwd primer (SEQ ID NO. 88)

<400>  74
tagtagttaa ttaagagaga atgaggccag cgctt                                  35


<210>  75
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human Fc rev primer (SEQ ID NO. 89)

<400>  75
ctactagttt aaactcattt acccggagac aggga                                  35


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'UTR fwd (SEQ ID NO. 90)

<400>  76
ttccttttcc agggtttgtg                                                   20


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'UTR rev (SEQ ID NO. 91)

<400>  77
gcctacaagg tggcttgaat                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDS fwd (SEQ ID NO. 92)

<400>  78
aagctgtgct cctgctcttg                                                   20


<210>  79
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CDS rev (SEQ ID NO. 93)

<400>  79
tcctgagtgc actgtgagat g                                                 21


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'UTR fwd (SEQ ID NO. 94)

<400>  80
ctgtagaggg cggtgacttt                                                   20


<210>  81
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'UTR rev (SEQ ID NO. 95)

<400>  81
agctgctccc aagtcctct                                                    19


<210>  82
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mACTB fwd (SEQ ID NO. 96)

<400>  82
ctaaggccaa ccgtgaaaag                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mACTB rev (SEQ ID NO. 97)

<400>  83
accagaggca tacagggaca                                                   20


<210>  84
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD141 1-42 aa (SEQ ID NO. 98)

<400>  84

Met Leu Gly Val Leu Val Leu Gly Ala Leu Ala Leu Ala Gly Leu Gly 
1               5                   10                  15      


Phe Pro Ala Pro Ala Glu Pro Gln Pro Gly Gly Ser Gln Cys Val Glu 
            20                  25                  30          


His Asp Cys Phe Ala Leu Tyr Pro Gly Pro 
        35                  40          


<210>  85
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD141 97-108 aa (SEQ ID NO. 99)

<400>  85

Gln Leu Pro Pro Gly Cys Gly Asp Pro Lys Arg Leu 
1               5                   10          


<210>  86
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD141 122-142 aa (SEQ ID NO. 100)

<400>  86

Thr Ser Tyr Ser Arg Trp Ala Arg Leu Asp Leu Asn Gly Ala Pro Leu 
1               5                   10                  15      


Cys Gly Pro Leu 
            20  


<210>  87
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain variable region of CRT2 (SEQ ID NO. 21)

<400>  87

Met Ala Glu Val Gln Gly Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Lys Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn 
            20                  25                  30          


Thr Tyr Ala Met His Trp Val Cys Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Thr Tyr Tyr 
    50                  55                  60                  


Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Gln 
65                  70                  75                  80  


Ser Met Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala 
                85                  90                  95      


Met Tyr Tyr Cys Val Arg Glu Gly Val Tyr Tyr Tyr Gly Ser Ser Gly 
            100                 105                 110         


Tyr Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
        115                 120                 125             


Ser Gly 
    130 


<210>  88
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1 of VH of CRT2 (SEQ ID NO. 22)

<400>  88

Gly Phe Thr Phe Asn Thr Tyr Ala 
1               5               


<210>  89
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2 of VH of CRT2 (SEQ ID NO. 23)

<400>  89

Ile Arg Ser Lys Ser Asn Asn Tyr Ala Thr 
1               5                   10  


<210>  90
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3 of VH of CRT2 (SEQ ID NO. 24)

<400>  90

Val Arg Glu Gly Val Tyr Tyr Tyr Gly Ser Ser Gly Tyr Tyr Ala Met 
1               5                   10                  15      


Asp Tyr 
        


<210>  91
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv of CRT2 (SEQ ID NO. 29)

<400>  91

Met Ala Glu Val Gln Gly Val Glu Ser Gly Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Lys Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn 
            20                  25                  30          


Thr Tyr Ala Met His Trp Val Cys Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Thr Tyr Tyr 
    50                  55                  60                  


Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Gln 
65                  70                  75                  80  


Ser Met Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala 
                85                  90                  95      


Met Tyr Tyr Cys Val Arg Glu Gly Val Tyr Tyr Tyr Gly Ser Ser Gly 
            100                 105                 110         


Tyr Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
        115                 120                 125             


Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro 
145                 150                 155                 160 


Gly Glu Lys Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr 
                165                 170                 175     


Met His Trp Phe Gln Gln Lys Pro Gly Thr Ser Pro Lys Leu Trp Ile 
            180                 185                 190         


Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly 
        195                 200                 205             


Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala 
    210                 215                 220                 


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Arg Ser Ser Tyr Pro Leu 
225                 230                 235                 240 


Thr Phe Gly Ala Pro Gly Lys Leu Glu Leu Lys Arg Ala Ala 
                245                 250                 


<210>  92
<211>  841
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR CRT2 (SEQ ID NO. 30)

<400>  92

Met Pro Arg Gly Trp Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser 
1               5                   10                  15      


Gly Phe Met Ser Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro 
            20                  25                  30          


Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu 
        35                  40                  45              


Thr Thr Thr Pro Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser 
    50                  55                  60                  


Gln His Gly Asn Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys 
65                  70                  75                  80  


Phe Thr Ser Thr Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser 
                85                  90                  95      


Ser Val Gln Ser Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro 
            100                 105                 110         


Ala Asn Val Ser Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro 
        115                 120                 125             


Gly Asn Val Ser Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser 
    130                 135                 140                 


Pro Thr Lys Pro Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys 
145                 150                 155                 160 


Ala Glu Ile Lys Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly 
                165                 170                 175     


Ile Cys Leu Glu Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys 
            180                 185                 190         


Asp Arg Gly Glu Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala 
        195                 200                 205             


Asp Ala Asp Ala Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser 
    210                 215                 220                 


Glu Val Arg Pro Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu 
225                 230                 235                 240 


Ile Ser Ser Lys Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys 
                245                 250                 255     


Lys Leu Gly Ile Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln 
            260                 265                 270         


Ser Tyr Ser Gln Lys Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu 
        275                 280                 285             


Leu Ala Val Leu Gly Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser 
    290                 295                 300                 


Trp Ser Pro Thr Gly Glu Arg Leu Glu Leu Glu Pro Val Asp Arg Val 
305                 310                 315                 320 


Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu 
                325                 330                 335     


Ser Asn Pro Gly Pro Gly Asn Met Gly Val Leu Leu Thr Gln Arg Thr 
            340                 345                 350         


Leu Leu Ser Leu Val Leu Ala Leu Leu Phe Pro Ser Met Ala Ser Met 
        355                 360                 365             


Ala Glu Val Gln Gly Val Glu Ser Gly Gly Gly Leu Val Gln Pro Lys 
    370                 375                 380                 


Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr 
385                 390                 395                 400 


Tyr Ala Met His Trp Val Cys Gln Ala Pro Gly Lys Gly Leu Glu Trp 
                405                 410                 415     


Val Ala Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Thr Tyr Tyr Ala 
            420                 425                 430         


Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Gln Ser 
        435                 440                 445             


Met Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Met 
    450                 455                 460                 


Tyr Tyr Cys Val Arg Glu Gly Val Tyr Tyr Tyr Gly Ser Ser Gly Tyr 
465                 470                 475                 480 


Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
                485                 490                 495     


Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            500                 505                 510         


Glu Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
        515                 520                 525             


Glu Lys Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
    530                 535                 540                 


His Trp Phe Gln Gln Lys Pro Gly Thr Ser Pro Lys Leu Trp Ile Tyr 
545                 550                 555                 560 


Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
                565                 570                 575     


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu 
            580                 585                 590         


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Arg Ser Ser Tyr Pro Leu Thr 
        595                 600                 605             


Phe Gly Ala Pro Gly Lys Leu Glu Leu Lys Arg Ala Ala Ile Glu Val 
    610                 615                 620                 


Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile 
625                 630                 635                 640 


Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly 
                645                 650                 655     


Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            660                 665                 670         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
        675                 680                 685             


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
    690                 695                 700                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
705                 710                 715                 720 


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
                725                 730                 735     


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            740                 745                 750         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        755                 760                 765             


Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln 
    770                 775                 780                 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
785                 790                 795                 800 


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                805                 810                 815     


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            820                 825                 830         


Leu His Met Gln Ala Leu Pro Pro Arg 
        835                 840     


<210>  93
<211>  401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VH of CRT2 (SEQ ID NO. 31)

<400>  93
gacgcttatc gatggccgag gtgcaggggg tggagtctgg tggaggattg gtgcagccta       60

aaggatcatt gaaactctca tgtgccgcct ctggtttcac cttcaatacc tatgccatgc      120

actgggtctg ccaggctcca ggaaagggtt tggaatgggt tgctcgcata agaagtaaaa      180

gtaataatta tgcaacatat tatgccgatt cagtgaaaga cagattcacc atctccagag      240

atgattcaca aagcatgctc tatctgcaaa tgaacaacct gaaaactgag gacacagcca      300

tgtattactg tgtgagagaa ggggtttatt actacggtag tagtgggtac tatgctatgg      360

actactgggg tcaaggaacc tcagtcaccg tctcctcagg t                          401


<210>  94
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding CDR1 of VH of CRT2 (SEQ ID NO. 32)

<400>  94
ggtttcacct tcaataccta tgcc                                              24


<210>  95
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding CDR2 of VH of CRT2 (SEQ ID NO. 33)

<400>  95
ataagaagta aaagtaataa ttatgcaaca                                        30


<210>  96
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding CDR3 of VH of CRT2 (SEQ ID NO. 34)

<400>  96
gtgagagaag gggtttatta ctacggtagt agtgggtact atgctatgga ctac             54


<210>  97
<211>  781
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding scFv of CRT2 (SEQ ID NO. 39)

<400>  97
gacgcttatc gatggccgag gtgcaggggg tggagtctgg tggaggattg gtgcagccta       60

aaggatcatt gaaactctca tgtgccgcct ctggtttcac cttcaatacc tatgccatgc      120

actgggtctg ccaggctcca ggaaagggtt tggaatgggt tgctcgcata agaagtaaaa      180

gtaataatta tgcaacatat tatgccgatt cagtgaaaga cagattcacc atctccagag      240

atgattcaca aagcatgctc tatctgcaaa tgaacaacct gaaaactgag gacacagcca      300

tgtattactg tgtgagagaa ggggtttatt actacggtag tagtgggtac tatgctatgg      360

actactgggg tcaaggaacc tcagtcaccg tctcctcagg ttcctcaggt ggaggcggtt      420

caggcggagg tggctctggc ggtggcggat cggaaattgt tctcacccag tctccagcaa      480

tcatgtctgc atctccaggg gagaaggtca ccataacctg cagtgccagc tcaagtgtaa      540

gttacatgca ctggttccag cagaagccag gcacttctcc caaactctgg atttatagca      600

catccaacct ggcttctgga gtccctgctc gcttcagtgg cagtggatct gggacctctt      660

actctctcac aatcagccga atggaggctg aagatgctgc cacttattac tgccagcaaa      720

ggagtagtta ccccctcacg ttcggtgctg ggaccaagct ggagctgaaa cgtgcggccg      780

c                                                                      781


<210>  98
<211>  2549
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding CAR CRT2 (SEQ ID NO. 40)

<400>  98
atgcctcgcg gctggacagc cctgtgcctg ctgtctctgc tgccatccgg cttcatgagc       60

ctggataata acggcacagc caccccagag ctgcctacac agggcacctt cagcaatgtg      120

tccacaaacg tgagctatca ggagaccaca accccttcta ccctgggatc cacaagcctg      180

caccccgtgt ctcagcacgg caacgaagcc accaccaaca tcaccgagac cacagtgaag      240

tttacctcca cctctgtgat tacctctgtg tacggaaata caaactccag cgtgcagtct      300

cagacatctg tgatctccac agtgtttaca acacctgcca atgtgtccac cccagagaca      360

accctgaagc ccagcctgtc tcctggaaat gtgtccgatc tgtctaccac ctccaccagc      420

ctggccacct ctcccaccaa gccctatacc tcctcttctc ccatcctgag cgatatcaaa      480

gccgagatca aatgcagcgg gattcgggaa gtgaaactga cacagggcat ctgcctggaa      540

cagaataaga catccagctg cgccgagttt aagaaagata gaggagaggg actggccagg      600

gtgctgtgtg gcgaagagca ggccgacgcc gatgccggcg cccaggtgtg ttccctgctg      660

ctggcccagt ctgaggtgcg cccccagtgc ctgctgctgg tgctggccaa tcggacagaa      720

attagcagca agctgcagct gatgaaaaaa caccagagcg atctgaaaaa gctgggcatc      780

ctggacttta ccgagcagga cgtggcctct caccagagct acagccagaa aacactgatc      840

gccctggtga ccagcggagc cctgctggcc gtgctgggca tcaccggata tttcctgatg      900

aataggcgca gctggagccc caccggcgag cggctggagc tggagcctgt cgaccgagtg      960

aagcagaccc tgaactttga tctgctgaag ctggccggcg acgtggagtc caaccccggg     1020

ccagggaata tgggcgtgct gctgacccag aggaccctgc tgagcctggt gctggccctg     1080

ctgtttccat ctatggcatc ggacgcttat cgatggccga ggtgcagggg gtggagtctg     1140

gtggaggatt ggtgcagcct aaaggatcat tgaaactctc atgtgccgcc tctggtttca     1200

ccttcaatac ctatgccatg cactgggtct gccaggctcc aggaaagggt ttggaatggg     1260

ttgctcgcat aagaagtaaa agtaataatt atgcaacata ttatgccgat tcagtgaaag     1320

acagattcac catctccaga gatgattcac aaagcatgct ctatctgcaa atgaacaacc     1380

tgaaaactga ggacacagcc atgtattact gtgtgagaga aggggtttat tactacggta     1440

gtagtgggta ctatgctatg gactactggg gtcaaggaac ctcagtcacc gtctcctcag     1500

gttcctcagg tggaggcggt tcaggcggag gtggctctgg cggtggcgga tcggaaattg     1560

ttctcaccca gtctccagca atcatgtctg catctccagg ggagaaggtc accataacct     1620

gcagtgccag ctcaagtgta agttacatgc actggttcca gcagaagcca ggcacttctc     1680

ccaaactctg gatttatagc acatccaacc tggcttctgg agtccctgct cgcttcagtg     1740

gcagtggatc tgggacctct tactctctca caatcagccg aatggaggct gaagatgctg     1800

ccacttatta ctgccagcaa aggagtagtt accccctcac gttcggtgct gggaccaagc     1860

tggagctgaa acgtgcggcc gcaattgaag ttatgtatcc tcctccttac ctagacaatg     1920

agaagagcaa tggaaccatt atccatgtga aagggaaaca cctttgtcca agtcccctat     1980

ttcccggacc ttctaagccc ttttgggtgc tggtggtggt tggtggagtc ctggcttgct     2040

atagcttgct agtaacagtg gcctttatta ttttctgggt gaggagtaag aggagcaggc     2100

tcctgcacag tgactacatg aacatgactc cccgccgccc cgggcccacc cgcaagcatt     2160

accagcccta tgccccacca cgcgacttcg cagcctatcg ctccagagtg aagttcagca     2220

ggagcgcaga cgcccccgcg taccagcagg gccagaacca gctctataac gagctcaatc     2280

taggacgaag agaggagtac gatgttttgg acaagagacg tggccgggac cctgagatgg     2340

ggggaaagcc gcagagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     2400

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     2460

ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac gacgcccttc     2520

acatgcaggc cctgccccct cgctaataa                                       2549


<210>  99
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRT3 scFv codon optimised human nucleotide sequence (SEQ ID NO. 
       101)

<400>  99
atggccgagg tgcagctgca gcagtctggc accgtgctgg ccaggcccgg agcaagcgtg       60

aagatgtcct gcaaggcctc tggctacacc ttcacaagct attggatgca ctgggtgaag      120

cagcgcccag gacagggcct ggagtggatc ggagcaatct accccggcaa ctccgacacc      180

tcttataatc agaagttcaa gggcaaggcc aagctgacag ccgtgacctc tacaagcacc      240

gcctacatgg agctgagcag cctgaccaac gaggatagcg ccgtgtttta ttgcacacac      300

tactatggct ccgactacgc tatggactat tggggccagg gcacctccgt gacagtgtct      360

agcggaggag gaggcagcgg aggaggaggc tccggcggcg gcggctctca gatcgtgctg      420

acccagagcc ctgccatcat gtccgcctct ctgggcgagc gggtgacaat gacctgtaca      480

gcctcctcta gcgtgtcctc tagctacctg cactggtatc agcagaagcc cggctcctct      540

cctaagctgt ggatctacag cacctccaat ctggcatccg gcgtgcctgc aaggttctct      600

ggcagcggct ccggcacctc ttacagcctg acaatcagca gcatggaggc agaggacgca      660

gcaacatact attgtcacca gtatcaccgg agcccaagaa cctttggcgg cggcacaaag      720

ctggagatca agcgggcggc cgca                                             744


<210>  100
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRT3 scFv murine codon optimised nucleotide sequence (SEQ ID NO. 
       102)

<400>  100
atggccgagg tgcagctgca gcagtctggc accgtgctgg ctcggcccgg agctagcgtg       60

aagatgtcct gcaaggcttc tggctacacc ttcacaagct actggatgca ctgggtgaag      120

cagcgcccag gacagggcct ggagtggatc ggcgccatct accccggaaa ctccgacacc      180

tcttacaacc agaagttcaa gggcaaggct aagctgacag ccgtgacctc tacaagcacc      240

gcttacatgg agctgagcag cctgaccaac gaggatagcg ccgtgtttta ctgcacacac      300

tactacggct ccgactacgc tatggattac tggggacagg gcacctccgt gacagtgtct      360

agcggaggag gaggaagcgg cggaggcggc agcggaggag gaggatctca gatcgtgctg      420

acccagtctc ctgctatcat gtccgcctct ctgggcgaga gggtgacaat gacctgtaca      480

gcctcctcta gcgtgtcctc tagctacctg cactggtatc agcagaagcc cggctcctct      540

cctaagctgt ggatctacag cacctccaac ctggcttccg gagtgcctgc tcggttctct      600

ggaagcggct ccggaacctc ttacagcctg acaatcagca gcatggaggc tgaggacgcc      660

gctacatact actgtcacca gtaccacagg agcccaagaa cctttggcgg aggcacaaag      720

ctggagatca agagggcggc cgca                                             744


<210>  101
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRT2 scFv human codon optimised nucleotide sequence (SEQ ID NO. 
       103)

<400>  101
atggcagagg tgcagggagt ggagagcgga ggcggcctgg tgcagcctaa gggctccctg       60

aagctgtctt gcgccgccag cggcttcacc tttaacacat atgcaatgca ctgggtgtgc      120

caggcaccag gcaagggcct ggagtgggtg gcacggatca gaagcaagtc caacaattat      180

gccacctact atgccgacag cgtgaaggat aggttcacaa tctcccgcga cgattctcag      240

agcatgctgt acctgcagat gaacaatctg aagaccgagg acacagccat gtactattgc      300

gtgcgggagg gcgtgtacta ttacggcagc tccggctatt acgctatgga ctactggggc      360

cagggcacca gcgtgacagt gtctagcgga ggaggaggct ccggaggagg aggctctggc      420

ggcggcggca gcgagatcgt gctgacccag tccccagcaa tcatgtccgc ctctccagga      480

gagaaggtga ccatcacatg ctccgcctcc tctagcgtgt cttatatgca ctggttccag      540

cagaagcccg gcacctctcc taagctgtgg atctacagca catccaatct ggcatccggc      600

gtgcccgcaa ggttttctgg cagcggctcc ggcacctctt atagcctgac aatcagccgg      660

atggaggcag aggacgcagc aacctattac tgtcagcaga gatcctctta ccctctgacc      720

tttggcgccg gcacaaagct ggagctgaag cgcgcggccg ca                         762


<210>  102
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRT2 scFv murine codon optimised nucleotide sequence (SEQ ID NO. 
       104)

<400>  102
atggctgagg tgcagggagt ggagagcgga ggaggcctgg tgcagcctaa gggctccctg       60

aagctgtctt gcgccgctag cggattcacc tttaacacat acgctatgca ctgggtgtgc      120

caggctccag gaaagggcct ggagtgggtg gccaggatca gaagcaagtc caacaactac      180

gctacctact acgccgacag cgtgaaggat cggttcacaa tctcccgcga cgattctcag      240

agcatgctgt acctgcagat gaacaacctg aagaccgagg acacagctat gtactactgc      300

gtgcgggagg gcgtgtacta ctacggcagc tccggatact acgctatgga ctactgggga      360

cagggcacct ccgtgacagt gtctagcgga ggaggaggct ccggaggagg aggctctgga      420

ggcggaggca gcgagatcgt gctgacccag tctccagcta tcatgtccgc ctctcccggc      480

gagaaggtga ccatcacatg ctccgcctcc tctagcgtgt cttacatgca ctggttccag      540

cagaagcccg gcacctctcc taagctgtgg atctacagca catccaacct ggctagcgga      600

gtgcccgctc ggttttctgg aagcggctcc ggaacctctt acagcctgac aatctccagg      660

atggaggctg aggacgccgc tacatactac tgtcagcaga gatcctctta ccctctgacc      720

tttggcgccg gaacaaagct ggagctgaag cgcgcggccg ca                         762


<210>  103
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus of CRT3 variants 1 and 2 heavy chain CDR1 (SEQ ID NO. 
       105)


<220>
<221>  Xaa
<222>  (1)..(1)
<223>  Xaa is GYTF or no amino acid

<220>
<221>  Xaa
<222>  (6)..(6)
<223>  Xaa is MH or no amino acid

<400>  103

Xaa Thr Ser Tyr Trp Xaa 
1               5       


<210>  104
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus of CRT3 variants 1 and 2 heavy chain CDR2 (SEQ ID NO. 
       106)


<220>
<221>  Xaa
<222>  (1)..(1)
<223>  Xaa is WIGA or no amino acid

<220>
<221>  Xaa
<222>  (10)..(10)
<223>  Xaa is S or no amino acid

<400>  104

Xaa Ile Tyr Pro Gly Asn Ser Asp Thr Xaa 
1               5                   10  


<210>  105
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus of CRT3 variants 1 and 2 heavy chain CDR3 (SEQ ID NO. 
       107)


<220>
<221>  Xaa
<222>  (12)..(12)
<223>  Xaa is Y or no amino acid

<400>  105

Thr His Tyr Tyr Gly Ser Asp Tyr Ala Met Asp Xaa 
1               5                   10          


<210>  106
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus of CRT3 variants 1 and 2 light chain CDR1 (SEQ ID NO. 
       108)


<220>
<221>  Xaa
<222>  (1)..(1)
<223>  Xaa is SSV or no amino acid

<220>
<221>  Xaa
<222>  (6)..(6)
<223>  Xaa is LHWY or no amino acid

<400>  106

Xaa Ser Ser Ser Tyr Xaa 
1               5       


<210>  107
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus of CRT3 variants 1 and 2 light chain CDR2 (SEQ ID NO. 
       109)


<220>
<221>  Xaa
<222>  (1)..(1)
<223>  Xaa is LWIY or no amino acid

<220>
<221>  Xaa
<222>  (5)..(5)
<223>  Xaa is NLA or no amino acid

<400>  107

Xaa Ser Thr Ser Xaa 
1               5   


<210>  108
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus of CRT3 variants 1 and 2 light chain CDR3 (SEQ ID NO. 
       110)


<220>
<221>  Xaa
<222>  (9)..(9)
<223>  Xaa is T or no amino acid

<400>  108

His Gln Tyr His Arg Ser Pro Arg Xaa 
1               5                   


<210>  109
<211>  216
<212>  DNA
<213>  Mus musculus

<400>  109
actactacca agccagtgct gcgaactccc tcacctgtgc accctaccgg gacatctcag       60

ccccagagac cagaagattg tcggccccgt ggctcagtga aggggaccgg attggacttc      120

gcctgtgata tttacatctg ggcacccttg gccggaatct gcgtggccct tctgctgtcc      180

ttgatcatca ctctcatctg ctaccacagg agccga                                216


<210>  110
<211>  123
<212>  DNA
<213>  Mus musculus

<400>  110
aatagtagaa ggaacagact ccttcaaagt gactacatga acatgactcc ccggaggcct       60

gggctcactc gaaagcctta ccagccctac gcccctgcca gagactttgc agcgtaccgc      120

ccc                                                                    123


<210>  111
<211>  135
<212>  DNA
<213>  Mus musculus

<400>  111
aaatggatca ggaaaaaatt cccccacata ttcaagcaac catttaagaa gaccactgga       60

gcagctcaag aggaagatgc ttgtagctgc cgatgtccac aggaagaaga aggaggagga      120

ggaggctatg agctg                                                       135


<210>  112
<211>  334
<212>  DNA
<213>  Mus musculus

<400>  112
agagcaaaat tcagcaggag tgcagagact gctgccaacc tgcaggaccc caaccagctc       60

tacaatgagc tcaatctagg gcgaagagag gaatatgacg tcttggagaa gaagcgggct      120

cgggatccag agatgggagg caaacagcag aggaggagga acccccagga aggcgtatac      180

aatgcactgc agaaagacaa gatggcagaa gcctacagtg agatcggcac aaaaggcgag      240

aggcggagag gcaaggggca cgatggcctt taccagggtc tcagcactgc caccaaggac      300

acctatgatg ccctgcatat gcagaccctg gccc                                  334


<210>  113
<211>  108
<212>  DNA
<213>  Mus musculus

<400>  113
cggaaggctt ggagattgcc taacactccc aaaccttgtt ggggaaacag cttcaggacc       60

ccgatccagg aggaacacac agacgcacac tttactctgg ccaagatc                   108


<210>  114
<211>  752
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CD8alpha hinge-CD8alpha transmembrane domain-CD28 
       costimulatory signal-CD3 zeta intracellular signalling domain 
       (SEQ ID NO. 116)

<400>  114
ggaggcacca agctggaaat caaacgtgcg gccgcaacta ctaccaagcc agtgctgcga       60

actccctcac ctgtgcaccc taccgggaca tctcagcccc agagaccaga agattgtcgg      120

ccccgtggct cagtgaaggg gaccggattg gacttcgcct gtgatattta catctgggca      180

cccttggccg gaatctgcgt ggcccttctg ctgtccttga tcatcactct catctgctac      240

cacaggagcc gaaatagtag aaggaacaga ctccttcaaa gtgactacat gaacatgact      300

ccccggaggc ctgggctcac tcgaaagcct taccagccct acgcccctgc cagagacttt      360

gcagcgtacc gccccagagc aaaattcagc aggagtgcag agactgctgc caacctgcag      420

gaccccaacc agctctacaa tgagctcaat ctagggcgaa gagaggaata tgacgtcttg      480

gagaagaagc gggctcggga tccagagatg ggaggcaaac agcagaggag gaggaacccc      540

caggaaggcg tatacaatgc actgcagaaa gacaagatgg cagaagccta cagtgagatc      600

ggcacaaaag gcgagaggcg gagaggcaag gggcacgatg gcctttacca gggtctcagc      660

actgccacca aggacaccta tgatgccctg catatgcaga ccctggcccc tcgctaataa      720

aagcttaaca cgagccatag atagaataaa ag                                    752


<210>  115
<211>  764
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CD8alpha hinge-CD8alpha transmembrane domain-41BB 
       costimulatory domain-CD3 zeta intracellular signalling domain 
       (SEQ ID NO. 117)

<400>  115
ggaggcacca agctggaaat caaacgtgcg gccgcaacta ctaccaagcc agtgctgcga       60

actccctcac ctgtgcaccc taccgggaca tctcagcccc agagaccaga agattgtcgg      120

ccccgtggct cagtgaaggg gaccggattg gacttcgcct gtgatattta catctgggca      180

cccttggccg gaatctgcgt ggcccttctg ctgtccttga tcatcactct catctgctac      240

cacaggagcc gaaaatggat caggaaaaaa ttcccccaca tattcaagca accatttaag      300

aagaccactg gagcagctca agaggaagat gcttgtagct gccgatgtcc acaggaagaa      360

gaaggaggag gaggaggcta tgagctgaga gcaaaattca gcaggagtgc agagactgct      420

gccaacctgc aggaccccaa ccagctctac aatgagctca atctagggcg aagagaggaa      480

tatgacgtct tggagaagaa gcgggctcgg gatccagaga tgggaggcaa acagcagagg      540

aggaggaacc cccaggaagg cgtatacaat gcactgcaga aagacaagat ggcagaagcc      600

tacagtgaga tcggcacaaa aggcgagagg cggagaggca aggggcacga tggcctttac      660

cagggtctca gcactgccac caaggacacc tatgatgccc tgcatatgca gaccctggcc      720

cctcgctaat aaaagcttaa cacgagccat agatagaata aaag                       764


<210>  116
<211>  737
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CD8alpha hinge-CD8alpha transmembrane domain-OX40 
       costimulatory domain-CD3 zeta intracellular signalling domain 
       (SEQ ID NO. 118)

<400>  116
ggaggcacca agctggaaat caaacgtgcg gccgcaacta ctaccaagcc agtgctgcga       60

actccctcac ctgtgcaccc taccgggaca tctcagcccc agagaccaga agattgtcgg      120

ccccgtggct cagtgaaggg gaccggattg gacttcgcct gtgatattta catctgggca      180

cccttggccg gaatctgcgt ggcccttctg ctgtccttga tcatcactct catctgctac      240

cacaggagcc gacggaaggc ttggagattg cctaacactc ccaaaccttg ttggggaaac      300

agcttcagga ccccgatcca ggaggaacac acagacgcac actttactct ggccaagatc      360

agagcaaaat tcagcaggag tgcagagact gctgccaacc tgcaggaccc caaccagctc      420

tacaatgagc tcaatctagg gcgaagagag gaatatgacg tcttggagaa gaagcgggct      480

cgggatccag agatgggagg caaacagcag aggaggagga acccccagga aggcgtatac      540

aatgcactgc agaaagacaa gatggcagaa gcctacagtg agatcggcac aaaaggcgag      600

aggcggagag gcaaggggca cgatggcctt taccagggtc tcagcactgc caccaaggac      660

acctatgatg ccctgcatat gcagaccctg gcccctcgct aataaaagct taacacgagc      720

catagataga ataaaag                                                     737


<210>  117
<211>  887
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CD8alpha hinge-CD8alpha transmembrane domain-CD28 and 41BB
       costimulatory domains-CD3 zeta intracellular signalling domain 
       (SEQ ID NO. 119)

<400>  117
ggaggcacca agctggaaat caaacgtgcg gccgcaacta ctaccaagcc agtgctgcga       60

actccctcac ctgtgcaccc taccgggaca tctcagcccc agagaccaga agattgtcgg      120

ccccgtggct cagtgaaggg gaccggattg gacttcgcct gtgatattta catctgggca      180

cccttggccg gaatctgcgt ggcccttctg ctgtccttga tcatcactct catctgctac      240

cacaggagcc gaaatagtag aaggaacaga ctccttcaaa gtgactacat gaacatgact      300

ccccggaggc ctgggctcac tcgaaagcct taccagccct acgcccctgc cagagacttt      360

gcagcgtacc gccccaaatg gatcaggaaa aaattccccc acatattcaa gcaaccattt      420

aagaagacca ctggagcagc tcaagaggaa gatgcttgta gctgccgatg tccacaggaa      480

gaagaaggag gaggaggagg ctatgagctg agagcaaaat tcagcaggag tgcagagact      540

gctgccaacc tgcaggaccc caaccagctc tacaatgagc tcaatctagg gcgaagagag      600

gaatatgacg tcttggagaa gaagcgggct cgggatccag agatgggagg caaacagcag      660

aggaggagga acccccagga aggcgtatac aatgcactgc agaaagacaa gatggcagaa      720

gcctacagtg agatcggcac aaaaggcgag aggcggagag gcaaggggca cgatggcctt      780

taccagggtc tcagcactgc caccaaggac acctatgatg ccctgcatat gcagaccctg      840

gcccctcgct aataaaagct taacacgagc catagataga ataaaag                    887


<210>  118
<211>  860
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CD8alpha hinge-CD8alpha transmembrane domain-CD28 and OX40
       costimulatory domains-CD3 zeta intracellular signalling domain 
       (SEQ ID NO. 120)

<400>  118
ggaggcacca agctggaaat caaacgtgcg gccgcaacta ctaccaagcc agtgctgcga       60

actccctcac ctgtgcaccc taccgggaca tctcagcccc agagaccaga agattgtcgg      120

ccccgtggct cagtgaaggg gaccggattg gacttcgcct gtgatattta catctgggca      180

cccttggccg gaatctgcgt ggcccttctg ctgtccttga tcatcactct catctgctac      240

cacaggagcc gaaatagtag aaggaacaga ctccttcaaa gtgactacat gaacatgact      300

ccccggaggc ctgggctcac tcgaaagcct taccagccct acgcccctgc cagagacttt      360

gcagcgtacc gcccccggaa ggcttggaga ttgcctaaca ctcccaaacc ttgttgggga      420

aacagcttca ggaccccgat ccaggaggaa cacacagacg cacactttac tctggccaag      480

atcagagcaa aattcagcag gagtgcagag actgctgcca acctgcagga ccccaaccag      540

ctctacaatg agctcaatct agggcgaaga gaggaatatg acgtcttgga gaagaagcgg      600

gctcgggatc cagagatggg aggcaaacag cagaggagga ggaaccccca ggaaggcgta      660

tacaatgcac tgcagaaaga caagatggca gaagcctaca gtgagatcgg cacaaaaggc      720

gagaggcgga gaggcaaggg gcacgatggc ctttaccagg gtctcagcac tgccaccaag      780

gacacctatg atgccctgca tatgcagacc ctggcccctc gctaataaaa gcttaacacg      840

agccatagat agaataaaag                                                  860


<210>  119
<211>  872
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine CD8alpha hinge-CD8alpha transmembrane domain- 41BB and 
       Ox40 costimulatory domains and CD3 zeta intracelluar signalling 
       domain (SEQ ID NO. 121)

<400>  119
ggaggcacca agctggaaat caaacgtgcg gccgcaacta ctaccaagcc agtgctgcga       60

actccctcac ctgtgcaccc taccgggaca tctcagcccc agagaccaga agattgtcgg      120

ccccgtggct cagtgaaggg gaccggattg gacttcgcct gtgatattta catctgggca      180

cccttggccg gaatctgcgt ggcccttctg ctgtccttga tcatcactct catctgctac      240

cacaggagcc gaaaatggat caggaaaaaa ttcccccaca tattcaagca accatttaag      300

aagaccactg gagcagctca agaggaagat gcttgtagct gccgatgtcc acaggaagaa      360

gaaggaggag gaggaggcta tgagctgcgg aaggcttgga gattgcctaa cactcccaaa      420

ccttgttggg gaaacagctt caggaccccg atccaggagg aacacacaga cgcacacttt      480

actctggcca agatcagagc aaaattcagc aggagtgcag agactgctgc caacctgcag      540

gaccccaacc agctctacaa tgagctcaat ctagggcgaa gagaggaata tgacgtcttg      600

gagaagaagc gggctcggga tccagagatg ggaggcaaac agcagaggag gaggaacccc      660

caggaaggcg tatacaatgc actgcagaaa gacaagatgg cagaagccta cagtgagatc      720

ggcacaaaag gcgagaggcg gagaggcaag gggcacgatg gcctttacca gggtctcagc      780

actgccacca aggacaccta tgatgccctg catatgcaga ccctggcccc tcgctaataa      840

aagcttaaca cgagccatag atagaataaa ag                                    872


