﻿               SEQUENCE LISTING

<110> Max-Delbrueck-Centrum fuer Molekulare Medizin
      Humboldt-Universität zu Berlin

<120> A transposon-based transfection system for primary cells

<130> MDC16343PCT

<160> 23

<170> BiSSAP 1.3

<210> 1
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> pT 4/5 consensus sequence outer DR

<400> 1
cagttgaagt cggaagttta catacacytw ag                                   32


<210> 2
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> pT4/5 consensus sequence inner DR

<400> 2
yccagtgggt cagaagtgta catacacgvk ct                                   32


<210> 3
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> pT4/5 left outer DR

<400> 3
cagttgaagt cggaagttta catacactta ag                                   32


<210> 4
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> pT4/5 right outer DR

<400> 4
cagttgaagt cggaagttta catacacctt ag                                   32


<210> 5
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> pT4/5 left inner DR

<400> 5
tccagtgggt cagaagtgta catacacgvk ct                                   32


<210> 6
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> pT4/5 right inner DR

<400> 6
cccagtgggt cagaagtgta catacacgvk ct                                   32


<210> 7
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<223> HDR

<400> 7
gtktacakac asd                                                       13


<210> 8
<211> 231
<212> DNA
<213> Artificial Sequence

<220> 
<223> Left IR/DR of pT4 with HDR

<400> 8
tacagttgaa gtcggaagtt tacatacacy twagttggag tcattaaaac tcgtttttca     60

actactccac aaatttcttg ttaacaaaca atagttttgg caagtcagtt aggacatcta    120

ctttgtgcat gacacaagtc atttttccaa caattgtkta cakacasdtt atttcactta    180

taattcactg tatcacaaty ccagtgggtc agaagtgtac atacacgvkc t             231


<210> 9
<211> 233
<212> DNA
<213> Artificial Sequence

<220> 
<223> Left IR/DR of pT5 with HDR

<400> 9
tatacagttg aagtcggaag tttacataca cytwagttgg agtcattaaa actcgttttt     60

caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag ttaggacatc    120

tactttgtgc atgacacaag tcatttttcc aacaattgtk tacakacasd ttatttcact    180

tataattcac tgtatcacaa tyccagtggg tcagaagtgt acatacacgv kct           233


<210> 10
<211> 232
<212> DNA
<213> Artificial Sequence

<220> 
<223> Right IR/DR of pT4 without HDR (right IR/DR comprises the reverse
      complement of the given sequences)

<400> 10
tacagttgaa gtcggaagtt tacatacacy twagccaaat acatttaaac tcactttttc     60

acaattcctg acatttaatc cgagtaaaga ttccctgtct taaggtcagt taggatcacc    120

actttatttt aagaatgtga aatatcagaa taatagtaga gagaatgatt catttcagct    180

tttatttctt tcatcacatt yccagtgggt cagaagtgta catacacgvk ct            232


<210> 11
<211> 233
<212> DNA
<213> Artificial Sequence

<220> 
<223> Right IR/DR of pT5 without HDR (right IR/DR comprises the reverse
      complement of the given sequences):

<400> 11
tatacagttg aagtcggaag tttacataca cytwagccaa atacatttaa actcactttt     60

tcacaattcc tgacatttaa tcctagtaaa aattccctgt cttaggtcag ttaggatcac    120

cactttattt taagaatgtg aaatatcaga ataatagtag agagaatgat tcatttcagc    180

ttttatttct ttcatcacat tyccagtggg tcagaagtgt acatacacgv kct           233


<210> 12
<211> 245
<212> DNA
<213> Artificial Sequence

<220> 
<223> Right IR/DR of pT4 with HDR (right IR/DR comprises the reverse
      complement of the given sequences)

<400> 12
tacagttgaa gtcggaagtt tacatacacy twagccaaat acatttaaac tcactttttc     60

acaattcctg acatttaatc cgagtaaaga ttccctgtct taaggtcagt taggatcacc    120

actttatttt aagaatgtga aatatcagaa taatagtaga gagaatgatg tktacakaca    180

sdtcatttca gcttttattt ctttcatcac attyccagtg ggtcagaagt gtacatacac    240

gvkct                                                                245


<210> 13
<211> 246
<212> DNA
<213> Artificial Sequence

<220> 
<223> Right IR/DR of pT5 with HDR (right IR/DR comprises the reverse
      complement of the given sequences)

<400> 13
tatacagttg aagtcggaag tttacataca cytwagccaa atacatttaa actcactttt     60

tcacaattcc tgacatttaa tcctagtaaa aattccctgt cttaggtcag ttaggatcac    120

cactttattt taagaatgtg aaatatcaga ataatagtag agagaatgat gtktacakac    180

asdtcatttc agcttttatt tctttcatca cattyccagt gggtcagaag tgtacataca    240

cgvkct                                                               246


<210> 14
<211> 6461
<212> DNA
<213> Artificial Sequence

<220> 
<223> plasmid for minicircle production

<400> 14
ttgcagaaga tcctgaacgt gcagaagaag ctgcctatca tccagaagat catcatcatg       60

gactctaaga ccgactacca gggcttccag agcatgtaca cattcgtgac atctcatctg      120

cctcctggct tcaacgagta cgacttcgtg ccagagtctt tcgacaggga caaaaccatt      180

gccctgatca tgaacagctc tgggtctacc gagatctgat atctctagag tcgagctagc      240

ttcgaattta aatcggatcc ctatacagtt gaagtcggaa gtttacatac accttagcca      300

aatacattta aactcacttt ttcacaattc ctgacattta atcctagtaa aaattccctg      360

tcttaggtca gttaggatca ccactttatt ttaagaatgt gaaatatcag aataatagta      420

gagagaatga ttcatttcag cttttatttc tttcatcaca ttcccagtgg gtcagaagtt      480

tacatacact caattagtat ttggtagcat tgcctttaaa ttgtttaact tgggtcaaac      540

atttcgagta gccttccaca agctagatcc tgggaataaa tggcggtaag atgctcgaat      600

tacacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat tgaattctta      660

cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac      720

catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg      780

gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc      840

gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt      900

cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg      960

ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag     1020

ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa     1080

gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt     1140

catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt     1200

gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta     1260

ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc     1320

cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatgcg     1380

gccgctggga gtggacacct gtggagagaa aggcaaagtg gatgtcagta agaccaatag     1440

gtgcctatca gaaacgcaag agtcttcatg cattaattac gcgttctgtc tcgacaagcc     1500

cagtttctat tggtctcctt aaacctgtct tgtaaccttg atacttacct gcccagtgcc     1560

tctccggaca aatgaaagac ccccgaggtg ggcagtcaat cactcagagg agaccctccc     1620

aaggaacagc gagaccacga gtcggatgca actgcaagag ggtttattga gaacacgggt     1680

acccgggcga cgcagtctat cggaggactg gcgcgccgag tgaggggttg tgggctcttt     1740

tattgagctc ggggagcaga agcgcgcgaa cagaagcgag aagcgaactg attggttagt     1800

tcaaataagg cacagggtca tttcaggtcc ttggggcacc ctggaaacat ctgatggttc     1860

tctagaaact gctgagggcg ggaccgcatc tggggaccat ctgttcttgg ccctgagccg     1920

gggcaggaac tgcttaccac agatatcctg tttggcccat attctgctgt tccaactgtt     1980

cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg cccatattct     2040

gctgtctctc tgttcctaac cttgatctga acttctcaag cttctaaagc catgacatca     2100

ttttctggaa ttttccaagc tgtttaaagg cacagtcaac ttagtgtatg taaacttctg     2160

acccactgga attgtgatac agtgaattat aagtgaaata atctgtctgt aaacaattgt     2220

tggaaaaatg acttgtgtca tgcacaaagt agatgtccta actgacttgc caaaactatt     2280

gtttgttaac aagaaatttg tggagtagtt gaaaaacgag ttttaatgac tccaacttaa     2340

gtgtatgtaa acttccgact tcaactgtat agggatccct gcaggagctc gtcgacccat     2400

gggggcccgc cccaactggg gtaacctttg agttctctca gttgggggta atcagcatca     2460

tgatgtggta ccacatcatg atgctgatta taagaatgcg gccgccacac tctagtggat     2520

ctcgagttaa taattcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa     2580

tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct     2640

tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg     2700

ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca     2760

tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac     2820

agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg     2880

gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag     2940

gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg     3000

gcaggagcaa ggtgtagatg acatggagat cctgccccgg cacttcgccc aatagcagcc     3060

agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg     3120

ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg     3180

tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc     3240

agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag     3300

aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat     3360

cagagcttga tcccctgcgc catcagatcc ttggcggcga gaaagccatc cagtttactt     3420

tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg     3480

tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc     3540

tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc     3600

agcaccgttt ctgcggactg gctttctacg tgctcgaggg gggccaaacg gtctccagct     3660

tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag     3720

aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc     3780

catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc     3840

gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct     3900

ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag     3960

cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa     4020

ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac     4080

aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaccaaaatc     4140

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct     4200

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta     4260

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc     4320

ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac     4380

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct     4440

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat     4500

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg     4560

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa     4620

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg     4680

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga     4740

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc     4800

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct     4860

gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct     4920

cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg     4980

atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc     5040

agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg     5100

actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt     5160

gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc     5220

agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg     5280

cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat     5340

gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta     5400

catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt     5460

ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg     5520

cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc     5580

gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg     5640

acaagcaaac atgctgtgcg acgctggcga tacattaccc tgttatccct agatgacatt     5700

accctgttat cccagatgac attaccctgt tatccctaga tgacattacc ctgttatccc     5760

tagatgacat ttaccctgtt atccctagat gacattaccc tgttatccca gatgacatta     5820

ccctgttatc cctagataca ttaccctgtt atcccagatg acataccctg ttatccctag     5880

atgacattac cctgttatcc cagatgacat taccctgtta tccctagata cattaccctg     5940

ttatcccaga tgacataccc tgttatccct agatgacatt accctgttat cccagatgac     6000

attaccctgt tatccctaga tacattaccc tgttatccca gatgacatac cctgttatcc     6060

ctagatgaca ttaccctgtt atcccagatg acattaccct gttatcccta gatacattac     6120

cctgttatcc cagatgacat accctgttat ccctagatga cattaccctg ttatcccaga     6180

tgacattacc ctgttatccc tagatacatt accctgttat cccagatgac ataccctgtt     6240

atccctagat gacattaccc tgttatccca gatgacatta ccctgttatc cctagataca     6300

ttaccctgtt atcccagatg acataccctg ttatccctag atgacattac cctgttatcc     6360

cagataaact caatgatgat gatgatgatg gtcgagactc agcggccgcg gtgccagggc     6420

gtgcccttgg gctccccggg cgcgactagt gaattcagat c                         6461


<210> 15
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> TCRα-specific antisense sequence

<400> 15
tgaaagttta ggttcgtatc tg                                             22


<210> 16
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> TCRβ-specific antisense sequence

<400> 16
tctgatggct caaacacagc ga                                             22


<210> 17
<211> 149
<212> DNA
<213> Artificial Sequence

<220> 
<223> miR-155

<400> 17
cttatcctct ggctgctgga ggcttgctga aggctgtatg ctgtgaaagt ttaggttcgt     60

atctgttttg gcctctgact gacagatacg aataaacttt cacaggacac aaggcctgtt    120

actagcactc acatggaaca aatggccac                                      149


<210> 18
<211> 157
<212> DNA
<213> Artificial Sequence

<220> 
<223> artificial miRNA

<400> 18
caagagaaca aagtggagtc tttgttgccc acacccagct tccctggctc tctgatggct     60

caaacacagc gagtacatga gacacgctgt gtttcagcca tcggtgagct tgggaagcat    120

ctgcagcaga gcctgcctgg tggcccctga gagattt                             157


<210> 19
<211> 312
<212> DNA
<213> Artificial Sequence

<220> 
<223> miRNA construct for silencing expression of human TCR a and b
      chains

<400> 19
cttatcctct ggctgctgga ggcttgctga aggctgtatg ctgtgaaagt ttaggttcgt     60

atctgttttg gcctctgact gacagatacg aataaacttt cacaggacac aaggcctgtt    120

actagcactc acatggaaca aatggccaca cgcgccaaga gaacaaagtg gagtctttgt    180

tgcccacacc cagcttccct ggctctctga tggctcaaac acagcgagta catgagacac    240

gctgtgtttc agccatcggt gagcttggga agcatctgca gcagagcctg cctggtggcc    300

cctgagagat tt                                                        312


<210> 20
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> MAGE-A1278 -specific peptide

<400> 20
Lys Val Leu Glu Tyr Val Ile Lys Val 
1               5                   

<210> 21
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> tyrosinase-specific control peptide Tyr369

<400> 21
Tyr Met Asp Gly Thr Met Ser Gln Val 
1               5                   

<210> 22
<211> 6417
<212> DNA
<213> Artificial Sequence

<220> 
<223> pSB-miR-TCR1367co

<400> 22
caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg       60

ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca      120

gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta      180

ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt      240

gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat      300

tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt      360

aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagcttga gaagttcaga      420

tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca      480

gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga      540

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc      600

ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc      660

tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc      720

gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc      780

ctccgataga ctgcgtcgcc cgggtacccg tgttctcaat aaaccctctt gcagttgcat      840

ccgactcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctgcccacct      900

cgggggtctt tcatttgtcc ggagaggcac tgggcaggta agtatcaagg ttacaagaca      960

ggtttaagga gaccaataga aactgggctt gtcgagacag aacgcgcctt atcctctggc     1020

tgctggaggc ttgctgaagg ctgtatgctg tgaaagttta ggttcgtatc tgttttggcc     1080

tctgactgac agatacgaat aaactttcac aggacacaag gcctgttact agcactcaca     1140

tggaacaaat ggccacacgc gccaagagaa caaagtggag tctttgttgc ccacacccag     1200

cttccctggc tctctgatgg ctcaaacaca gcgagtacat gagacacgct gtgtttcagc     1260

catcggtgag cttgggaagc atctgcagca gagcctgcct ggtggcccct gagagattta     1320

cgcgtaatta atgcatgaag actcttgcgt ttctgatagg cacctattgg tcttactgac     1380

atccactttg cctttctctc cacaggtgtc cactcccagc ggccgccacc atgggaatca     1440

gactgctgtg cagagtggcc ttctgcttcc tggccgtggg cctggtggac gtgaaagtga     1500

cccagagcag cagatacctc gtgaagcgga ccggcgagaa ggtgttcctg gaatgcgtgc     1560

aggacatgga ccacgagaat atgttctggt acagacagga ccccggcctg ggcctgcggc     1620

tgatctactt cagctacgac gtgaagatga aggaaaaggg cgacatcccc gagggctaca     1680

gcgtgtccag agagaagaaa gagcggttca gcctgatcct ggaaagcgcc agcaccaacc     1740

agaccagcat gtacctgtgc gccagcagag gcctggccgg ctacgagcag tattttggcc     1800

ctggcacccg gctgaccgtg accgaggatc tgaagaacgt gttcccccca gaggtggccg     1860

tgttcgagcc ttctgaggcc gagatcagcc acacccagaa agccaccctc gtgtgtctgg     1920

ccaccggctt ctaccccgac cacgtggaac tgtcttggtg ggtcaacggc aaagaggtgc     1980

acagcggcgt gtccaccgat ccccagcctc tgaaagaaca gcccgccctg aacgacagcc     2040

ggtactgcct gtccagcaga ctgagagtgt ccgccacctt ctggcagaac ccccggaacc     2100

acttcagatg ccaggtgcag ttctacggcc tgagcgagaa cgacgagtgg acccaggaca     2160

gagccaagcc cgtgacacag atcgtgtctg ccgaagcctg gggcagagcc gattgcggct     2220

ttaccagcga gagctaccag cagggcgtgc tgagcgccac catcctgtac gagatcctgc     2280

tgggcaaggc caccctgtac gccgtgctgg tgtctgccct ggtgctgatg gccatggtca     2340

agcggaagga ctccagaggc ggaagcggcg ccaccaactt cagcctgctg aaacaggccg     2400

gcgacgtgga agagaaccct ggccccatga agacttcgcc ggcttcagct tcctgttcct     2460

gtggctgcag ctggactgca tgagcagagg cgaggacgtg gaacagagcc tgtttctgag     2520

cgtgcgcgag ggcgacagca gcgtgatcaa ttgcacctac accgacagct ccagcaccta     2580

cctgtactgg tacaagcagg aacctggcgc cggactgcag ctgctgacct acatcttcag     2640

caacatggac atgaagcagg accagagact gaccgtgctg ctgaacaaga aggacaagca     2700

cctgagcctg cggatcgccg atacccagac aggcgacagc gccatctact tttgcgccga     2760

gagcatcggc agcaacagcg gctacgccct gaatttcggc aagggcacaa gcctgctcgt     2820

gaccccccac atccagaacc ctgaccctgc cgtgtaccag ctgcgggaca gcaagagcag     2880

cgacaagagc gtgtgcctgt tcaccgactt cgacagccag accaacgtgt cccagagcaa     2940

ggacagcgac gtgtacatca ccgacaagac agtgctggac atgcggagca tggacttcaa     3000

gagcaactcc gccgtggctt ggagcaacaa gagcgacttc gcctgcgcca acgccttcaa     3060

caacagcatt atccctgagg acacattctt cccaagcccc gagagcagct gtgacgtgaa     3120

gctggtggaa aagagcttcg agacagacac caacctgaac ttccagaacc tgagcgtgat     3180

cggcttcaga atcctgctgc tgaaggtggc cggcttcaac ctgctgatga ccctgagact     3240

gtggtccagc tgaattcaat aaaatatctt tattttcatt acatctgtgt gttggttttt     3300

tgtgtgtaat tcggatctag cttgtggaag gctactcgaa atgtttgacc caagttaaac     3360

aatttaaagg caatgctacc aaatactaat tgagtgtatg taaacttctg acccactggg     3420

aatgtgatga aagaaataaa agctgaaatg aatcattctc tctactatta ttctgatatt     3480

tcacattctt aaaataaagt ggtgatccta actgacctaa gacagggaat ttttactagg     3540

attaaatgtc aggaattgtg aaaaagtgag tttaaatgta tttggctaag gtgtatgtaa     3600

acttccgact tcaactgtat agggatcctc tagctagagt cgacctcgag ggggggcccg     3660

gtacccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca     3720

tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga     3780

agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg     3840

cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc     3900

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac     3960

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata     4020

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     4080

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     4140

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     4200

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     4260

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     4320

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     4380

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     4440

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     4500

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg     4560

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     4620

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     4680

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac     4740

gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc     4800

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag     4860

taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt     4920

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     4980

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     5040

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     5100

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     5160

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     5220

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     5280

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     5340

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     5400

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     5460

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     5520

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     5580

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     5640

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     5700

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat     5760

tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa     5820

aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt     5880

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc     5940

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc     6000

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg     6060

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga     6120

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc     6180

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg     6240

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt     6300

aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag     6360

ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctg        6417


<210> 23
<211> 1824
<212> DNA
<213> Artificial Sequence

<220> 
<223> TCR1367, codon-optized, minimally murinized, additional cysteine
      bridge

<400> 23
atgggaatca gactgctgtg cagagtggcc ttctgcttcc tggccgtggg cctggtggac      60

gtgaaagtga cccagagcag cagatacctc gtgaagcgga ccggcgagaa ggtgttcctg     120

gaatgcgtgc aggacatgga ccacgagaat atgttctggt acagacagga ccccggcctg     180

ggcctgcggc tgatctactt cagctacgac gtgaagatga aggaaaaggg cgacatcccc     240

gagggctaca gcgtgtccag agagaagaaa gagcggttca gcctgatcct ggaaagcgcc     300

agcaccaacc agaccagcat gtacctgtgc gccagcagag gcctggccgg ctacgagcag     360

tattttggcc ctggcacccg gctgaccgtg accgaggacc tgaagaacgt gttccccccc     420

gaggtggccg tgttcgagcc cagcaaggcc gagatcgccc acacccagaa agccaccctg     480

gtgtgcctgg ccaccggctt ctaccccgac cacgtggaac tgtcttggtg ggtgaacggc     540

aaagaggtgc acagcggcgt gtgtaccgac ccccagcccc tgaaagagca gcctgccctg     600

aacgactccc ggtactgcct gagcagccgg ctgagagtgt ccgccacctt ctggcagaac     660

ccccggaacc acttcagatg ccaggtgcag ttctacggcc tgagcgagaa cgacgagtgg     720

acccaggacc gggccaagcc cgtgacccag attgtgtctg ccgaggcctg gggcagagct     780

gattgtggca tcaccagcgc cagctaccac cagggcgtgc tgagcgccac catcctgtac     840

gagatcctgc tgggcaaggc caccctgtac gccgtgctgg tgtccgccct ggtgctgatg     900

gccatggtga aacggaagga cagcagaggc ggcagcggcg ccaccaactt tagcctgctg     960

aaacaggccg gcgacgtgga agagaaccct ggccccatga agaccttcgc cggcttcagc    1020

ttcctgttcc tgtggctgca gctggactgc atgagcaggg gcgaggacgt ggaacagagc    1080

ctgtttctga gcgtgcgcga gggcgacagc agcgtgatca attgcaccta caccgacagc    1140

tccagcacct acctgtactg gtacaagcag gaacctggcg ccggactgca gctgctgacc    1200

tacatcttca gcaacatgga catgaagcag gaccagagac tgaccgtgct gctgaacaag    1260

aaggacaagc acctgagcct gcggatcgcc gatacccaga caggcgacag cgccatctac    1320

ttttgcgccg agagcatcgg cagcaacagc ggctacgccc tgaacttcgg caagggcaca    1380

agcctgctcg tgacccctca catccagaac cccgaccccg ccgtgtacca gctgcgggac    1440

agcaagagca gcgacaagag cgtgtgcctg ttcaccgact tcgacagcca gaccaacgtg    1500

tcccagagca aggacagcga cgtgtacatc accgacaagt gcgtgctgga catgcggagc    1560

atggacttca agagcaactc cgccgtggcc tggtccaaca agagcgactt cgcctgcgcc    1620

aacgccttca acaacagcat catccccgag gacacattct tccccagctc cgacgtgccc    1680

tgcgacgtga agctggtgga aaagagcttc gagacagaca ccaacctgaa cttccagaac    1740

ctgagcgtga tcggcttcag aatcctgctg ctgaaggtgg ctggcttcaa cctgctgatg    1800

accctgcggc tgtggagcag ctga                                           1824


