                         SEQUENCE LISTING

<110>  CITY OF HOPE
       SHI, Yanhong
 
<120>  TLX AND MIR-219 AS POTENTIAL THERAPEUTIC TARGETS FOR 
       NEURODEVELOPMENTAL DISORDERS

<130>  054435-8146.WO00

<140>  PCT/US17/21962
<141>  2017-03-10

<150>  US 62/306,631
<151>  2016-03-10

<150>  US 62/307,367
<151>  2016-03-11

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-219 antisense northern blot probe

<400>  1
agaattgcgt ttggacaatc a                                                 21


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 antisense northern blot probe

<400>  2
tatggaacgc ttctcgaatt                                                   20


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mTLX forward RT-PCR primer

<400>  3
gtctttacaa gatcagctga tg                                                22


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mTLX reverse RT-PCR primer

<400>  4
atgtcactgg atttgtacat atc                                               23


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pri-miR-219-1 forward RT-PCR primer

<400>  5
tttcccacgc cagacattca c                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pri-miR-219-1 reverse RT-PCR primer

<400>  6
gatccccaac ttctctcaag c                                                 21


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pri-miR-219-2 forward RT-PCR primer

<400>  7
ttgccgagct tctgcgaggt a                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pri-miR-219-2 reverse RT-PCR primer

<400>  8
tgtcccctct ttgcatgcca g                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDGFRalpha forward RT-PCR primer

<400>  9
caaacctgac catgccacca g                                                 21


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDGFRalpha reverse RT-PCR primer

<400>  10
tctcgatggc actctcttcc g                                                 21


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RORbeta forward RT-PCR primer

<400>  11
tacgtggtgg agttcgccaa g                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RORbeta reverse RT-PCR primer

<400>  12
cccatgcaag ttgcagactg c                                                 21


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LMO3 forward RT-PCR primer

<400>  13
gtttggtgta acgggaaact gcg                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LMO3 reverse RT-PCR primer

<400>  14
tcctcgtagt ctgtctggca aag                                               23


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGA2 forward RT-PCR primer

<400>  15
acatcagccc agggacaacc t                                                 21


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HMGA2 reverse RT-PCR primer

<400>  16
caagagtccg cagaggagga t                                                 21


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EphrinB2 forward RT-PCR primer

<400>  17
ttcagcccta acctctgggg t                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EphrinB2 reverse RT-PCR primer

<400>  18
aacccaggag attgttcccg g                                                 21


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mGAPDH forward RT-PCR primer

<400>  19
catcaccatc ttccaggagc                                                   20


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mGAPDH reverse RT-PCR primer

<400>  20
gctgtagccg tattcattgt c                                                 21


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pri-miR-219-2 forward RT-PCR primer

<400>  21
tacgcagctc ccgagatctg gtg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pri-miR-219-2 reverse RT-PCR primer

<400>  22
cagcgtggac ctcgtctctg tag                                               23


<210>  23
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pre-miR-219-2 forward RT-PCR primer

<400>  23
ctgattgtcc aaacgcaatt cttg                                              24


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pre-miR-219-2 reverse RT-PCR primer

<400>  24
cagatgtcca gccacaattc tc                                                22


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDGFRalpha forward RT-PCR primer

<400>  25
gtggcctgga cgaacagaga ct                                                22


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDGFRalpha reverse RT-PCR primer

<400>  26
ggaacctgtc tcgatggcac tc                                                22


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  m TLX forward RT-PCR primer

<400>  27
ggttcagaca gctccgatta gac                                               23


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  m TLX reverse RT-PCR primer

<400>  28
tggagagcgg caatggcggc agc                                               23


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  beta-Actin forward RT-PCR primer

<400>  29
ccgagcgtgg ctacagcttc                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  beta-Actin reverse RT-PCR primer

<400>  30
acctggccgt caggcagctc                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTLX forward RT-PCR primer

<400>  31
ctaagagtgt gccagccttc                                                   20


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hTLX reverse RT-PCR primer

<400>  32
tgttagcatc aaccggaatg g                                                 21


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hGAPDH forward RT-PCR primer

<400>  33
cctgttcgac agtcagccg                                                    19


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hGAPDH reverse RT-PCR primer

<400>  34
cgaccaaatc cgttgactc                                                    19


<210>  35
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TuD-miR-219

<400>  35
gacggcgcta ggatcatcaa cctcgagcgc tagcaagtat tctggtcaca gaatacaacg       60

tcgaccacta gtcaagatga tcctagcgcc gtctttttt                              99


<210>  36
<211>  1158
<212>  DNA
<213>  Homo sapiens

<400>  36
atgagcaagc cagccggatc aacaagccgc attttagata tcccctgcaa agtgtgtggc       60

gaccgcagct cggggaagca ctacggggtc tacgcctgcg acggctgctc aggttttttc      120

aaacggagca tccgaaggaa taggacctat gtctgcaaat ctggaaacca gggaggctgt      180

ccggtggaca agacgcacag aaaccagtgc agggcgtgtc ggctgaagaa gtgtttggaa      240

gtcaacatga acaaagacgc cgtgcagcac gagcgggggc ctcggacgtc caccatccgc      300

aagcaagtgg ccctctactt ccgtggacac aaggaggaga acggggccgc cgcgcacttt      360

ccctcggcgg cgctccctgc gccggccttc ttcaccgcgg tcacgcagct ggagccgcac      420

ggcctggagc tggccgcggt gtccaccact ccagagcggc agaccctcgt gagcctggct      480

cagcccacgc ccaagtaccc ccatgaagtg aatgggaccc caatgtatct ctatgaagtg      540

gccacggagt cggtgtgtga atcagctgcc agacttctct tcatgagcat caagtgggct      600

aagagtgtgc cagccttctc cacgctgtct ttgcaagacc agctgatgct tttggaagat      660

gcttggagag aactgtttgt tctaggaata gcacaatggg ccattccggt tgatgctaac      720

actctactgg ctgtatctgg catgaacggt gacaacacag attcccagaa gctgaacaag      780

atcatatctg aaatacaggc tttacaagag gtggtggctc gatttagaca actccggtta      840

gatgctactg aatttgcctg tctaaaatgc atcgtcactt tcaaagccgt tcctacacat      900

agtggttctg aactgagaag tttccggaat gctgccgcca ttgcagccct tcaagatgag      960

gctcagctaa cgctcaacag ctacatccat accagatatc ccactcaacc ctgtcgcttt     1020

ggaaaactcc tgttgctttt gccagcttta cgttctatta gcccatcaac tatagaagaa     1080

gtgtttttca aaaaaaccat cggcaatgtg ccaattacaa gactgctttc agatatgtac     1140

aaatccagtg atatctaa                                                   1158


<210>  37
<211>  385
<212>  PRT
<213>  Homo sapiens

<400>  37

Met Ser Lys Pro Ala Gly Ser Thr Ser Arg Ile Leu Asp Ile Pro Cys 
1               5                   10                  15      


Lys Val Cys Gly Asp Arg Ser Ser Gly Lys His Tyr Gly Val Tyr Ala 
            20                  25                  30          


Cys Asp Gly Cys Ser Gly Phe Phe Lys Arg Ser Ile Arg Arg Asn Arg 
        35                  40                  45              


Thr Tyr Val Cys Lys Ser Gly Asn Gln Gly Gly Cys Pro Val Asp Lys 
    50                  55                  60                  


Thr His Arg Asn Gln Cys Arg Ala Cys Arg Leu Lys Lys Cys Leu Glu 
65                  70                  75                  80  


Val Asn Met Asn Lys Asp Ala Val Gln His Glu Arg Gly Pro Arg Thr 
                85                  90                  95      


Ser Thr Ile Arg Lys Gln Val Ala Leu Tyr Phe Arg Gly His Lys Glu 
            100                 105                 110         


Glu Asn Gly Ala Ala Ala His Phe Pro Ser Ala Ala Leu Pro Ala Pro 
        115                 120                 125             


Ala Phe Phe Thr Ala Val Thr Gln Leu Glu Pro His Gly Leu Glu Leu 
    130                 135                 140                 


Ala Ala Val Ser Thr Thr Pro Glu Arg Gln Thr Leu Val Ser Leu Ala 
145                 150                 155                 160 


Gln Pro Thr Pro Lys Tyr Pro His Glu Val Asn Gly Thr Pro Met Tyr 
                165                 170                 175     


Leu Tyr Glu Val Ala Thr Glu Ser Val Cys Glu Ser Ala Ala Arg Leu 
            180                 185                 190         


Leu Phe Met Ser Ile Lys Trp Ala Lys Ser Val Pro Ala Phe Ser Thr 
        195                 200                 205             


Leu Ser Leu Gln Asp Gln Leu Met Leu Leu Glu Asp Ala Trp Arg Glu 
    210                 215                 220                 


Leu Phe Val Leu Gly Ile Ala Gln Trp Ala Ile Pro Val Asp Ala Asn 
225                 230                 235                 240 


Thr Leu Leu Ala Val Ser Gly Met Asn Gly Asp Asn Thr Asp Ser Gln 
                245                 250                 255     


Lys Leu Asn Lys Ile Ile Ser Glu Ile Gln Ala Leu Gln Glu Val Val 
            260                 265                 270         


Ala Arg Phe Arg Gln Leu Arg Leu Asp Ala Thr Glu Phe Ala Cys Leu 
        275                 280                 285             


Lys Cys Ile Val Thr Phe Lys Ala Val Pro Thr His Ser Gly Ser Glu 
    290                 295                 300                 


Leu Arg Ser Phe Arg Asn Ala Ala Ala Ile Ala Ala Leu Gln Asp Glu 
305                 310                 315                 320 


Ala Gln Leu Thr Leu Asn Ser Tyr Ile His Thr Arg Tyr Pro Thr Gln 
                325                 330                 335     


Pro Cys Arg Phe Gly Lys Leu Leu Leu Leu Leu Pro Ala Leu Arg Ser 
            340                 345                 350         


Ile Ser Pro Ser Thr Ile Glu Glu Val Phe Phe Lys Lys Thr Ile Gly 
        355                 360                 365             


Asn Val Pro Ile Thr Arg Leu Leu Ser Asp Met Tyr Lys Ser Ser Asp 
    370                 375                 380                 


Ile 
385 


<210>  38
<211>  57
<212>  DNA
<213>  Homo sapiens

<400>  38
ggaaaactcc tgttgctttt gccagcttta cgttctatta gcccatcaac tatagaa          57


<210>  39
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  39

Gly Lys Leu Leu Leu Leu Leu Pro Ala Leu Arg Ser Ile Ser Pro Ser 
1               5                   10                  15      


Thr Ile Glu 
            


<210>  40
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-219-5P target site

<400>  40
gacaatca                                                                 8


<210>  41
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated miR-219-5P target site

<400>  41
gatcgtca                                                                 8


<210>  42
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TLX Dpi peptide

<400>  42

Gly Lys Leu Leu Leu Leu Leu Pro Ala Leu Arg Ser Ile Ser Pro Ser 
1               5                   10                  15      


Thr Ile Glu 
            


<210>  43
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TLX control peptide

<400>  43

Lys Ser Val Pro Ala Phe Ser Thr Leu Ser Leu Gln Asp Gln Leu Met 
1               5                   10                  15      


Leu Leu Glu Asp Ala Trp Arg 
            20              


<210>  44
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo of TuD-miR-219

<400>  44
tcgaagaatt gcgttctgat ggacaatca                                         29


<210>  45
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo of TuD-miR-219

<400>  45
ctagtgattg tccatcagaa cgcaattct                                         29


<210>  46
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  46
ttcatagagc tcacaccggc ttgtccacct tac                                    33


<210>  47
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  47
ttcatactcg aggaggatac ggaaagaggc gag                                    33


<210>  48
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  48
gatagctagc aatggcaact ccaagcgtgc t                                      31


<210>  49
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  49
atgaggccga ggtggggctg agtctagaga tc                                     32


