                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Jarjour, Jordan
       Astrakhan, Alexander
 
<120>  GENOME EDITED IMMUNE EFFECTOR CELLS

<130>  BLBD-065/02WO

<150>  US 62/307,245
<151>  2016-03-11

<150>  US 63/322,604
<151>  2016-04-14

<160>  69    

<170>  PatentIn version 3.5

<210>  1
<211>  909
<212>  DNA
<213>  Ophiostoma novo-ulmi

<400>  1
atggcataca tgtcgcgcag agagtccatc aacccatgga ttctgactgg tttcgctgat       60

gccgaaggat ccttcttgct gagaatccga aacaataaca agagctccgt gggttactct      120

accgagttgg gctttcaaat cactctgcac aacaaggaca aatcgattct ggagaatatc      180

cagtcgactt ggaaggtcgg cgtgattgct aactcaggcg acaatgccgt cagtctgaaa      240

gttacgcgtt tcgaagattt gaaagtgatt atcgaccact tcgagaaata tccgctgatt      300

acccagaaat tgggcgatta caagttgttt aaacaggcat tcagcgtcat ggagaacaaa      360

gaacatctta aggagaatgg gattaaggag ctcgtacgaa tcaaagctaa gatgaattgg      420

ggtctcactg acgaattgaa aaaagcattt ccagagaaca ttagcaaaga gcgccccctt      480

atcaataaga acattccgaa tttcaaatgg ctggctggat tcacatctgg tgaaggctgc      540

ttctttgtga acttgatcaa gtccaaatct aagctgggtg tacaggttca attggtcttc      600

agcattactc agcacatcag agacaagaac ctgatgaatt cattgataac atacctaggc      660

tgtggttaca tcaaagagaa gaacaagtcc gagttcagtt ggctcgactt tgtggttacc      720

aaattcagcg atatcaacga caagatcatt ccggtattcc aggaaaatac tctgattggc      780

gtcaaactcg aggactttga agattggtgc aaggttgcca aattgatcga agagaagaaa      840

cacctgaccg aatccggttt ggatgagatt aagaaaatca agctgaacat gaacaaaggt      900

cgtgtcttc                                                              909


<210>  2
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  2

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  3
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  3
tgtctgccta ttcaccgatt tt                                                22


<210>  4
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  4
ctagcacagt tttgtctgtg at                                                22


<210>  5
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered I-OnuI variant

<400>  5

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
            20                  25                  30          


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Ser Gly Asp Arg Ala Val Met Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Asn Ala 
            180                 185                 190         


Lys Val Tyr Val Gly Leu Arg Phe Ser Ile Thr Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
    210                 215                 220                 


Arg Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Glu Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  6
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered I-OnuI variant

<400>  6

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
            20                  25                  30          


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Ser Gly Asp Arg Ala Val Met Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Asn Ala 
            180                 185                 190         


Lys Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
    210                 215                 220                 


Trp Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Glu Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  7
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered I-OnuI variant

<400>  7

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
            20                  25                  30          


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala 
                165                 170                 175     


Gly Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala 
            180                 185                 190         


Lys Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
    210                 215                 220                 


Trp Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  8
<211>  7554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW790 - Adeno-associated virus (AAV) plasmid containing 
       a promoter, a fluorescent reporter transgene and a 
       polyadenylation signal

<400>  8
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg       60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact      120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg      180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg      240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac      300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca      360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga      420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc      480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct      540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg      600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct      660

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc      720

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc      780

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat      840

taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg      900

cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact      960

ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctaccgcgg     1020

atgactaaca atcaggggga tgtgttggta gagctaatgg ctttctgtct gtcccttccc     1080

agcaaaggaa ctatgcctta gggccttcac ccagagtgat gtcaggctgc ccaagcatga     1140

ggagggaagt aggcagaatc ctctggagcc aaagctctgg atgtctctcc cctctgacca     1200

tggagcccac ccctgctcca ctgctccagg gacagcccta tgctgcaggc agctctgccc     1260

ccactcagca tcccaggggc tgatttcttt ggttttggat ccagctggat gtctgcattg     1320

ccgaggccac cagggctggc tcagcaactg tcggggaatc accagggtct gagaaatctt     1380

gtgcgcatgt gaggggctgt gggagcagag aacactgggt gggaaattct aatccccacc     1440

ctgctggaaa ctctctggtg gccccaacat gctaatcctc cggcaaacct ctgtttcctc     1500

ctcaaaaggc aggaggtcgg aaagaataaa caatgagagt cacattaaaa acacaaaatc     1560

ctacggaaat actgaagaat gagtctcagc actaaggaaa agcctccagc agctcctgct     1620

ttctgagggt gaaggataga cgctgtggct ctgcatgact cactagcact ctatcacggc     1680

catattctgg cagggtcagt ggctccaact aacatttgtt tggtacttta cagtttatta     1740

aatagatgtt tatatggaga agctctcatt tctttctcag aagagcctgg ctaggaaggt     1800

ggatgaggca ccatattcat tttgcaggtg aaattcctga gatgtaagga gctgctgtga     1860

cttgctcaag gccttatatc aagtaaacgg tagcgctggg gcttagacgc aggtgttctg     1920

atttatagtt caaaacctct atcaatgaga gagcaatctc ctggtaatgt gatagatttc     1980

ccaacttaat gccaacatac cataaacctc ccattctgct aatgcccagc ctaagttggg     2040

gagaccactc cagattccaa gatgtacagt ttgctttgct gggccttttt cccatgcctg     2100

cctttactct gccagagtta tattgctggg gttttgaaga agatcctatt aaataaaaga     2160

ataagcagta ttattaagta gccctgcatt tcaggtttcc ttgagtggca ggccaggcct     2220

ggcgtgaacg ttcactgaaa tcatggcctc ttggccaaga ttgatagctt gtgcctgtcc     2280

ctgagtccca gtccatcacg agcagctggt ttctaagatg ctatttcccg tataaagcat     2340

gagaccgtga cttgccagcc ccacagagcc ccgcccttgt ccatcactgg catctggact     2400

ccagcctggg ttggggcaaa gagggaaatg agatcatgtc ctaaccctga tcctcttgtc     2460

ccacagatat ccagaaccct gaccctgccg tgtaccagct gagagactct aaatccagtg     2520

acaagtctgt ctgcctatac gcgtaggctc cggtgcccgt cagtgggcag agcgcacatc     2580

gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaacggtg cctagagaag     2640

gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg     2700

tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt     2760

tgccgccaga acacacctgc aggtaatacg actcactata gggtccactg ccgccaccat     2820

ggctagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga     2880

caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac     2940

catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac     3000

tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt     3060

caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aggacggggg     3120

cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa     3180

gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg     3240

ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat     3300

ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc     3360

caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga     3420

aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag     3480

atactgcgac ctccctagca aactggggca caagcttaat tgagcggccg cgctttattt     3540

gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta     3600

acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt     3660

aaagctcacc ggttttgatt ctcaaacaaa tgtgtcacaa agtaaggatt ctgatgtgta     3720

tatcacagac aaaactgtgc tagacatgag gtctatggac ttcaagagca acagtgctgt     3780

ggcctggagc aacaaatctg actttgcatg tgcaaacgcc ttcaacaaca gcattattcc     3840

agaagacacc ttcttcccca gcccaggtaa gggcagcttt ggtgccttcg caggctgttt     3900

ccttgcttca ggaatggcca ggttctgccc agagctctgg tcaatgatgt ctaaaactcc     3960

tctgattggt ggtctcggcc ttatccattg ccaccaaaac cctcttttta ctaagaaaca     4020

gtgagccttg ttctggcagt ccagagaatg acacgggaaa aaagcagatg aagagaaggt     4080

ggcaggagag ggcacgtggc ccagcctcag tctctccaac tgagttcctg cctgcctgcc     4140

tttgctcaga ctgtttgccc cttactgctc ttctaggcct cattctaagc cccttctcca     4200

agttgcctct ccttatttct ccctgtctgc caaaaaatct ttcccagctc actaagtcag     4260

tctcacgcag tcactcatta acccaccaat cactgattgt gccggcacat gaatgcacca     4320

ggtgttgaag tggaggaatt aaaaagtcag atgaggggtg tgcccagagg aagcaccatt     4380

ctagttgggg gagcccatct gtcagctggg aaaagtccaa ataacttcag attggaatgt     4440

gttttaactc agggttgaga aaacagccac cttcaggaca aaagtcaggg aagggctctc     4500

tgaagaaatg ctacttgaag ataccagccc taccaagggc agggagagga ccctatagag     4560

gcctgggaca ggagctcaat gagaaaggag aagagcagca ggcatgagtt gaatgaagga     4620

ggcagggccg ggtcacaggg cctgtagata agtagcatgg cgggttaatc attaactaca     4680

aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg     4740

ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc     4800

gagcgcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg     4860

cgcagcctga atggcgaatg gcgattccgt tgcaatggct ggcggtaata ttgttctgga     4920

tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa     4980

tcaaagaagt attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg     5040

cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc     5100

tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt     5160

gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     5220

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     5280

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     5340

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     5400

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     5460

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     5520

cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     5580

agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa     5640

tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata     5700

tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct     5760

caggcaatga cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat     5820

gaatttatca gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct     5880

ttctcacccg tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga     5940

gggttctaaa aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca     6000

gggtcataat gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa     6060

ttttgctaat tctttgcctt gcctgtatga tttattggat gttggaatcg cctgatgcgg     6120

tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca     6180

atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg     6240

ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg     6300

agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc     6360

gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt     6420

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     6480

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg     6540

aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc     6600

cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg     6660

ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt     6720

cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta     6780

ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat     6840

gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga     6900

gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca     6960

acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact     7020

cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc     7080

acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact     7140

ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt     7200

ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt     7260

gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt     7320

atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata     7380

ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag     7440

attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat     7500

ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga cccc           7554


<210>  9
<211>  7698
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW851 - Adeno-associated virus (AAV) plasmid containing 
       a promoter, a fluorescent reporter transgene and a 
       polyadenylation signal

<400>  9
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg       60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact      120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg      180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg      240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac      300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca      360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga      420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc      480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct      540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg      600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct      660

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc      720

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc      780

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat      840

taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg      900

cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact      960

ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctaccgcgg     1020

atgactaaca atcaggggga tgtgttggta gagctaatgg ctttctgtct gtcccttccc     1080

agcaaaggaa ctatgcctta gggccttcac ccagagtgat gtcaggctgc ccaagcatga     1140

ggagggaagt aggcagaatc ctctggagcc aaagctctgg atgtctctcc cctctgacca     1200

tggagcccac ccctgctcca ctgctccagg gacagcccta tgctgcaggc agctctgccc     1260

ccactcagca tcccaggggc tgatttcttt ggttttggat ccagctggat gtctgcattg     1320

ccgaggccac cagggctggc tcagcaactg tcggggaatc accagggtct gagaaatctt     1380

gtgcgcatgt gaggggctgt gggagcagag aacactgggt gggaaattct aatccccacc     1440

ctgctggaaa ctctctggtg gccccaacat gctaatcctc cggcaaacct ctgtttcctc     1500

ctcaaaaggc aggaggtcgg aaagaataaa caatgagagt cacattaaaa acacaaaatc     1560

ctacggaaat actgaagaat gagtctcagc actaaggaaa agcctccagc agctcctgct     1620

ttctgagggt gaaggataga cgctgtggct ctgcatgact cactagcact ctatcacggc     1680

catattctgg cagggtcagt ggctccaact aacatttgtt tggtacttta cagtttatta     1740

aatagatgtt tatatggaga agctctcatt tctttctcag aagagcctgg ctaggaaggt     1800

ggatgaggca ccatattcat tttgcaggtg aaattcctga gatgtaagga gctgctgtga     1860

cttgctcaag gccttatatc aagtaaacgg tagcgctggg gcttagacgc aggtgttctg     1920

atttatagtt caaaacctct atcaatgaga gagcaatctc ctggtaatgt gatagatttc     1980

ccaacttaat gccaacatac cataaacctc ccattctgct aatgcccagc ctaagttggg     2040

gagaccactc cagattccaa gatgtacagt ttgctttgct gggccttttt cccatgcctg     2100

cctttactct gccagagtta tattgctggg gttttgaaga agatcctatt aaataaaaga     2160

ataagcagta ttattaagta gccctgcatt tcaggtttcc ttgagtggca ggccaggcct     2220

ggcgtgaacg ttcactgaaa tcatggcctc ttggccaaga ttgatagctt gtgcctgtcc     2280

ctgagtccca gtccatcacg agcagctggt ttctaagatg ctatttcccg tataaagcat     2340

gagaccgtga cttgccagcc ccacagagcc ccgcccttgt ccatcactgg catctggact     2400

ccagcctggg ttggggcaaa gagggaaatg agatcatgtc ctaaccctga tcctcttgtc     2460

ccacagatat ccagaaccct gaccctgccg tgtaccagct gagagactct aaatccagtg     2520

acaagtctgt ctgcctatac gcgtgaacag agaaacagga gaatatgggc caaacaggat     2580

atctgtggta agcagttcct gccccggctc agggccaaga acagttggaa cagcagaata     2640

tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga     2700

tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg     2760

gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc     2820

tcgcttctgt tcgcgcgctt ctgctccccg agctctatat aagcagagct cgtttagtga     2880

accgtcagat cgcctggaga cgccatccac gctgttttga cttccataga aggatctcga     2940

ggccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga     3000

gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc     3060

cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg     3120

gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca     3180

catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac     3240

catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga     3300

caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct     3360

ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca     3420

gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca     3480

gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga     3540

caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca     3600

catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta     3660

caagtaagcg gccgcgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca     3720

ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc     3780

agggggagat gtgggaggtt ttttaaagct caccggtttt gattctcaaa caaatgtgtc     3840

acaaagtaag gattctgatg tgtatatcac agacaaaact gtgctagaca tgaggtctat     3900

ggacttcaag agcaacagtg ctgtggcctg gagcaacaaa tctgactttg catgtgcaaa     3960

cgccttcaac aacagcatta ttccagaaga caccttcttc cccagcccag gtaagggcag     4020

ctttggtgcc ttcgcaggct gtttccttgc ttcaggaatg gccaggttct gcccagagct     4080

ctggtcaatg atgtctaaaa ctcctctgat tggtggtctc ggccttatcc attgccacca     4140

aaaccctctt tttactaaga aacagtgagc cttgttctgg cagtccagag aatgacacgg     4200

gaaaaaagca gatgaagaga aggtggcagg agagggcacg tggcccagcc tcagtctctc     4260

caactgagtt cctgcctgcc tgcctttgct cagactgttt gccccttact gctcttctag     4320

gcctcattct aagccccttc tccaagttgc ctctccttat ttctccctgt ctgccaaaaa     4380

atctttccca gctcactaag tcagtctcac gcagtcactc attaacccac caatcactga     4440

ttgtgccggc acatgaatgc accaggtgtt gaagtggagg aattaaaaag tcagatgagg     4500

ggtgtgccca gaggaagcac cattctagtt gggggagccc atctgtcagc tgggaaaagt     4560

ccaaataact tcagattgga atgtgtttta actcagggtt gagaaaacag ccaccttcag     4620

gacaaaagtc agggaagggc tctctgaaga aatgctactt gaagatacca gccctaccaa     4680

gggcagggag aggaccctat agaggcctgg gacaggagct caatgagaaa ggagaagagc     4740

agcaggcatg agttgaatga aggaggcagg gccgggtcac agggcctgta gataagtagc     4800

atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc     4860

tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg     4920

cccgggcggc ctcagtgagc gagcgagcgc gccagctggc gtaatagcga agaggcccgc     4980

accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgatt ccgttgcaat     5040

ggctggcggt aatattgttc tggatattac cagcaaggcc gatagtttga gttcttctac     5100

tcaggcaagt gatgttatta ctaatcaaag aagtattgcg acaacggtta atttgcgtga     5160

tggacagact cttttactcg gtggcctcac tgattataaa aacacttctc aggattctgg     5220

cgtaccgttc ctgtctaaaa tccctttaat cggcctcctg tttagctccc gctctgattc     5280

taacgaggaa agcacgttat acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg     5340

gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg     5400

ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc     5460

cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc     5520

tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga     5580

cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa     5640

ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga     5700

tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca     5760

aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt     5820

tctgattatc aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg     5880

attctcttgt ttgctccaga ctctcaggca atgacctgat agcctttgta gagacctctc     5940

aaaaatagct accctctccg gcatgaattt atcagctaga acggttgaat atcatattga     6000

tggtgatttg actgtctccg gcctttctca cccgtttgaa tctttaccta cacattactc     6060

aggcattgca tttaaaatat atgagggttc taaaaatttt tatccttgcg ttgaaataaa     6120

ggcttctccc gcaaaagtat tacagggtca taatgttttt ggtacaaccg atttagcttt     6180

atgctctgag gctttattgc ttaattttgc taattctttg ccttgcctgt atgatttatt     6240

ggatgttgga atcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac     6300

cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga     6360

cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac     6420

agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg     6480

aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata     6540

ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt     6600

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa     6660

atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt     6720

attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa     6780

gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac     6840

agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt     6900

aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt     6960

cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat     7020

cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac     7080

actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg     7140

cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc     7200

ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa     7260

ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag     7320

gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct     7380

gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat     7440

ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa     7500

cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac     7560

caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc     7620

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     7680

cactgagcgt cagacccc                                                   7698


<210>  10
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCRalpha I-OnuI megaTAL target site

<400>  10
aaatccagtg acaagtctgt ctgcctattc accgatttt                              39


<210>  11
<211>  887
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered TCRalpha I-OnuI megaTAL

<400>  11

Met Gly Ser Cys Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Pro Pro 
1               5                   10                  15      


Lys Lys Lys Arg Lys Val Val Asp Leu Arg Thr Leu Gly Tyr Ser Gln 
            20                  25                  30          


Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val Ala Gln 
        35                  40                  45              


His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His Ile Val 
    50                  55                  60                  


Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val Thr Tyr 
65                  70                  75                  80  


Gln His Ile Ile Thr Ala Leu Pro Glu Ala Thr His Glu Asp Ile Val 
                85                  90                  95      


Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala Leu Leu 
            100                 105                 110         


Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp Thr Gly 
        115                 120                 125             


Gln Leu Val Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Met Glu Ala 
    130                 135                 140                 


Val His Ala Ser Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn Leu Thr 
145                 150                 155                 160 


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
                165                 170                 175     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            180                 185                 190         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
        195                 200                 205             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    210                 215                 220                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly 
225                 230                 235                 240 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                245                 250                 255     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            260                 265                 270         


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        275                 280                 285             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    290                 295                 300                 


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
305                 310                 315                 320 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                325                 330                 335     


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            340                 345                 350         


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
        355                 360                 365             


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
    370                 375                 380                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
385                 390                 395                 400 


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
                405                 410                 415     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
            420                 425                 430         


Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala 
        435                 440                 445             


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
    450                 455                 460                 


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
465                 470                 475                 480 


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
                485                 490                 495     


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly 
            500                 505                 510         


Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp 
        515                 520                 525             


Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys 
    530                 535                 540                 


Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys Lys Gly Leu Pro His 
545                 550                 555                 560 


Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg Ile Gly Glu Arg Thr 
                565                 570                 575     


Ser His Arg Val Ala Ile Ser Arg Val Gly Gly Ser Ser Arg Arg Glu 
            580                 585                 590         


Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
        595                 600                 605             


Phe Ile Leu Asp Ile Arg Asn Arg Asn Asn Glu Ser Asn Arg Tyr Arg 
    610                 615                 620                 


Thr Ser Leu Arg Phe Gln Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
625                 630                 635                 640 


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Thr Asn Ser 
                645                 650                 655     


Gly Asp Arg Ala Val Met Leu Arg Val Thr Arg Phe Glu Asp Leu Lys 
            660                 665                 670         


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
        675                 680                 685             


Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Met Glu Asn Lys 
    690                 695                 700                 


Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
705                 710                 715                 720 


Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala Phe Pro Glu 
                725                 730                 735     


Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Phe 
            740                 745                 750         


Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Tyr Phe Gly Val Asn 
        755                 760                 765             


Leu Lys Lys Val Lys Gly Asn Ala Lys Val Tyr Val Gly Leu Arg Phe 
    770                 775                 780                 


Ser Ile Ser Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile 
785                 790                 795                 800 


Thr Tyr Leu Gly Cys Gly Ser Ile Trp Glu Lys Asn Lys Ser Glu Phe 
                805                 810                 815     


Ser Trp Leu Glu Phe Val Val Thr Lys Phe Ser Asp Ile Asn Asp Lys 
            820                 825                 830         


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
        835                 840                 845             


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
    850                 855                 860                 


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
865                 870                 875                 880 


Met Asn Lys Gly Arg Val Phe 
                885         


<210>  12
<211>  7389
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW1019 - An adeno-associated virus (AAV) plasmid 
       containing a promoter, a transgene encoding a chimeric antigen 
       receptor (CAR), and a polyadenylation signal

<400>  12
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg       60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact      120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg      180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg      240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac      300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca      360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga      420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc      480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct      540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg      600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct      660

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc      720

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc      780

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat      840

taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg      900

cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact      960

ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctacgctgg     1020

ggcttagacg caggtgttct gatttatagt tcaaaacctc tatcaatgag agagcaatct     1080

cctggtaatg tgatagattt cccaacttaa tgccaacata ccataaacct cccattctgc     1140

taatgcccag cctaagttgg ggagaccact ccagattcca agatgtacag tttgctttgc     1200

tgggcctttt tcccatgcct gcctttactc tgccagagtt atattgctgg ggttttgaag     1260

aagatcctat taaataaaag aataagcagt attattaagt agccctgcat ttcaggtttc     1320

cttgagtggc aggccaggcc tggcgtgaac gttcactgaa atcatggcct cttggccaag     1380

attgatagct tgtgcctgtc cctgagtccc agtccatcac gagcagctgg tttctaagat     1440

gctatttccc gtataaagca tgagaccgtg acttgccagc cccacagagc cccgcccttg     1500

tccatcactg gcatctggac tccagcctgg gttggggcaa agagggaaat gagatcatgt     1560

cctaaccctg atcctcttgt cccacagata tccagaaccc tgaccctgcc gtgtaccagc     1620

tgagagactc taaatccagt gacaagtctg tctgcctata cgcgtgatcc atcgattagt     1680

ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca     1740

ccagctgaag cctatagagt acgagccata gatagaataa aagattttat ttagtctcca     1800

gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctaggat caaggttagg     1860

aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc     1920

ggctcagggc caagaacagt tggaacagca gaatatgggc caaacaggat atctgtggta     1980

agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct     2040

cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc     2100

tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct     2160

ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgacgcgtc atagccacca     2220

tggccttacc agtgaccgcc ttgctcctgc cgctggcctt gctgctccac gccgccaggc     2280

cggacatcca gatgacacag actacatcct ccctgtctgc ctctctggga gacagagtca     2340

ccatcagttg cagggcaagt caggacatta gtaaatattt aaattggtat cagcagaaac     2400

cagatggaac tgttaaactc ctgatctacc atacatcaag attacactca ggagtcccat     2460

caaggttcag tggcagtggg tctggaacag attattctct caccattagc aacctggagc     2520

aagaagatat tgccacttac ttttgccaac agggtaatac gcttccgtac acgttcggag     2580

gggggaccaa gctggagatc acaggtggcg gtggctccgg cggtggtggg tctggtggcg     2640

gcggaagcga ggtgaaactg caggagtcag gacctggcct ggtggcgccc tcacagagcc     2700

tgtccgtcac atgcactgtc tcaggggtct cattacccga ctatggtgta agctggattc     2760

gccagcctcc acgaaagggt ctggagtggc tgggagtaat atggggtagt gaaaccacat     2820

actataattc agctctcaaa tccagactga ccatcatcaa ggacaactcc aagagccaag     2880

ttttcttaaa aatgaacagt ctgcaaactg atgacacagc catttactac tgtgccaaac     2940

attattacta cggtggtagc tatgctatgg actactgggg tcaaggaacc tcggtcaccg     3000

tctcctcaac cacgacgcca gcgccgcgac caccaacacc ggcgcccacc atcgcgtcgc     3060

agcccctgtc cctgcgccca gaggcgtgcc ggccagcggc ggggggcgca gtgcacacga     3120

gggggctgga cttcgcctgt gatatctaca tctgggcgcc cttggccggg acttgtgggg     3180

tccttctcct gtcactggtg atcacccttt actgcaaacg gggcagaaag aaactcctgt     3240

atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa gatggctgta     3300

gctgccgatt tccagaagaa gaagaaggag gatgtgaact gagagtgaag ttcagcagga     3360

gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataacgag ctcaatctag     3420

gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct gagatggggg     3480

gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag aaagataaga     3540

tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc aaggggcacg     3600

atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc cttcacatgc     3660

aggccctgcc ccctcgctaa gcggccgcgc tttatttgtg aaatttgtga tgctattgct     3720

ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt     3780

atgtttcagg ttcaggggga gatgtgggag gttttttaaa gctcaccggt tttgattctc     3840

aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa actgtgctag     3900

acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac aaatctgact     3960

ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc ttccccagcc     4020

caggtaaggg cagctttggt gccttcgcag gctgtttcct tgcttcagga atggccaggt     4080

tctgcccaga gctctggtca atgatgtcta aaactcctct gattggtggt ctcggcctta     4140

tccattgcca ccaaaaccct ctttttacta agaaacagtg agccttgttc tggcagtcca     4200

gagaatgaca cgggaaaaaa gcagatgaag agaaggtggc aggagagggc acgtggccca     4260

gcctcagtct ctccaactga gttcctgcct gcctgccttt gctcagactg tttgcccctt     4320

actgctcttc taggcctcat tctaagcccc ttctccaagt tgcctctcct tatttctccc     4380

tgtctgccaa aaaatctttc ccagctcact aagtcagtct cacgcagtca ctcattaacc     4440

caccaatcac tgattgtgcc ggcacatgaa tgcaccaggt agataagtag catggcgggt     4500

taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc     4560

gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg     4620

cctcagtgag cgagcgagcg cgccagctgg cgtaatagcg aagaggcccg caccgatcgc     4680

ccttcccaac agttgcgcag cctgaatggc gaatggcgat tccgttgcaa tggctggcgg     4740

taatattgtt ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag     4800

tgatgttatt actaatcaaa gaagtattgc gacaacggtt aatttgcgtg atggacagac     4860

tcttttactc ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt     4920

cctgtctaaa atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga     4980

aagcacgtta tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa     5040

gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc     5100

ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag     5160

ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca     5220

aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc     5280

gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa     5340

cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct     5400

attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa     5460

cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat     5520

caaccggggt acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg     5580

tttgctccag actctcaggc aatgacctga tagcctttgt agagacctct caaaaatagc     5640

taccctctcc ggcatgaatt tatcagctag aacggttgaa tatcatattg atggtgattt     5700

gactgtctcc ggcctttctc acccgtttga atctttacct acacattact caggcattgc     5760

atttaaaata tatgagggtt ctaaaaattt ttatccttgc gttgaaataa aggcttctcc     5820

cgcaaaagta ttacagggtc ataatgtttt tggtacaacc gatttagctt tatgctctga     5880

ggctttattg cttaattttg ctaattcttt gccttgcctg tatgatttat tggatgttgg     5940

aatcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg     6000

tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca     6060

acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct     6120

gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg     6180

agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt     6240

tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt     6300

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa     6360

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt     6420

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat     6480

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag     6540

atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg     6600

ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata     6660

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat     6720

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc     6780

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg     6840

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac     6900

gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact     6960

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa     7020

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct     7080

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc     7140

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga     7200

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac     7260

tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag     7320

atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg     7380

tcagacccc                                                             7389


<210>  13
<211>  7336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW1018 - An adeno-associated virus (AAV) plasmid 
       containing a viral self-cleaving peptide, e.g., T2A peptide, a 
       fluorescent reporter transgene and a polyadenylation signal

<400>  13
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg       60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact      120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg      180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg      240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac      300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca      360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga      420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc      480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct      540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg      600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct      660

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc      720

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc      780

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat      840

taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg      900

cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact      960

ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctaccgcgg     1020

atgactaaca atcaggggga tgtgttggta gagctaatgg ctttctgtct gtcccttccc     1080

agcaaaggaa ctatgcctta gggccttcac ccagagtgat gtcaggctgc ccaagcatga     1140

ggagggaagt aggcagaatc ctctggagcc aaagctctgg atgtctctcc cctctgacca     1200

tggagcccac ccctgctcca ctgctccagg gacagcccta tgctgcaggc agctctgccc     1260

ccactcagca tcccaggggc tgatttcttt ggttttggat ccagctggat gtctgcattg     1320

ccgaggccac cagggctggc tcagcaactg tcggggaatc accagggtct gagaaatctt     1380

gtgcgcatgt gaggggctgt gggagcagag aacactgggt gggaaattct aatccccacc     1440

ctgctggaaa ctctctggtg gccccaacat gctaatcctc cggcaaacct ctgtttcctc     1500

ctcaaaaggc aggaggtcgg aaagaataaa caatgagagt cacattaaaa acacaaaatc     1560

ctacggaaat actgaagaat gagtctcagc actaaggaaa agcctccagc agctcctgct     1620

ttctgagggt gaaggataga cgctgtggct ctgcatgact cactagcact ctatcacggc     1680

catattctgg cagggtcagt ggctccaact aacatttgtt tggtacttta cagtttatta     1740

aatagatgtt tatatggaga agctctcatt tctttctcag aagagcctgg ctaggaaggt     1800

ggatgaggca ccatattcat tttgcaggtg aaattcctga gatgtaagga gctgctgtga     1860

cttgctcaag gccttatatc aagtaaacgg tagcgctggg gcttagacgc aggtgttctg     1920

atttatagtt caaaacctct atcaatgaga gagcaatctc ctggtaatgt gatagatttc     1980

ccaacttaat gccaacatac cataaacctc ccattctgct aatgcccagc ctaagttggg     2040

gagaccactc cagattccaa gatgtacagt ttgctttgct gggccttttt cccatgcctg     2100

cctttactct gccagagtta tattgctggg gttttgaaga agatcctatt aaataaaaga     2160

ataagcagta ttattaagta gccctgcatt tcaggtttcc ttgagtggca ggccaggcct     2220

ggcgtgaacg ttcactgaaa tcatggcctc ttggccaaga ttgatagctt gtgcctgtcc     2280

ctgagtccca gtccatcacg agcagctggt ttctaagatg ctatttcccg tataaagcat     2340

gagaccgtga cttgccagcc ccacagagcc ccgcccttgt ccatcactgg catctggact     2400

ccagcctggg ttggggcaaa gagggaaatg agatcatgtc ctaaccctga tcctcttgtc     2460

ccacagatat ccagaaccct gaccctgccg tgtaccagct gagagactct aaatccagtg     2520

acaagtctgt ctgcctatac ggtgagggca gaggaagtct tctaacatgc ggtgacgtgg     2580

aggagaatcc gggccctacc atggctagcg agctgattaa ggagaacatg cacatgaagc     2640

tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca     2700

agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct     2760

tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca     2820

cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag     2880

tcaccacata cgaggacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg     2940

gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga     3000

tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg     3060

gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa     3120

acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct     3180

actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc     3240

agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta     3300

attgagcggc cgcgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt     3360

ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag     3420

ggggagatgt gggaggtttt ttaaagctca ccggttttga ttctcaaaca aatgtgtcac     3480

aaagtaagga ttctgatgtg tatatcacag acaaaactgt gctagacatg aggtctatgg     3540

acttcaagag caacagtgct gtggcctgga gcaacaaatc tgactttgca tgtgcaaacg     3600

ccttcaacaa cagcattatt ccagaagaca ccttcttccc cagcccaggt aagggcagct     3660

ttggtgcctt cgcaggctgt ttccttgctt caggaatggc caggttctgc ccagagctct     3720

ggtcaatgat gtctaaaact cctctgattg gtggtctcgg ccttatccat tgccaccaaa     3780

accctctttt tactaagaaa cagtgagcct tgttctggca gtccagagaa tgacacggga     3840

aaaaagcaga tgaagagaag gtggcaggag agggcacgtg gcccagcctc agtctctcca     3900

actgagttcc tgcctgcctg cctttgctca gactgtttgc cccttactgc tcttctaggc     3960

ctcattctaa gccccttctc caagttgcct ctccttattt ctccctgtct gccaaaaaat     4020

ctttcccagc tcactaagtc agtctcacgc agtcactcat taacccacca atcactgatt     4080

gtgccggcac atgaatgcac caggtgttga agtggaggaa ttaaaaagtc agatgagggg     4140

tgtgcccaga ggaagcacca ttctagttgg gggagcccat ctgtcagctg ggaaaagtcc     4200

aaataacttc agattggaat gtgttttaac tcagggttga gaaaacagcc accttcagga     4260

caaaagtcag ggaagggctc tctgaagaaa tgctacttga agataccagc cctaccaagg     4320

gcagggagag gaccctatag aggcctggga caggagctca atgagaaagg agaagagcag     4380

caggcatgag ttgaatgaag gaggcagggc cgggtcacag ggcctgtaga taagtagcat     4440

ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg     4500

cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc     4560

cgggcggcct cagtgagcga gcgagcgcgc cagctggcgt aatagcgaag aggcccgcac     4620

cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgattcc gttgcaatgg     4680

ctggcggtaa tattgttctg gatattacca gcaaggccga tagtttgagt tcttctactc     4740

aggcaagtga tgttattact aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg     4800

gacagactct tttactcggt ggcctcactg attataaaaa cacttctcag gattctggcg     4860

taccgttcct gtctaaaatc cctttaatcg gcctcctgtt tagctcccgc tctgattcta     4920

acgaggaaag cacgttatac gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc     4980

gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc     5040

ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc     5100

cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc     5160

gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg     5220

gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact     5280

ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt     5340

tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa     5400

atattaacgt ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc     5460

tgattatcaa ccggggtaca tatgattgac atgctagttt tacgattacc gttcatcgat     5520

tctcttgttt gctccagact ctcaggcaat gacctgatag cctttgtaga gacctctcaa     5580

aaatagctac cctctccggc atgaatttat cagctagaac ggttgaatat catattgatg     5640

gtgatttgac tgtctccggc ctttctcacc cgtttgaatc tttacctaca cattactcag     5700

gcattgcatt taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg     5760

cttctcccgc aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat     5820

gctctgaggc tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg     5880

atgttggaat cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg     5940

catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca     6000

cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag     6060

acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa     6120

acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat     6180

aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg     6240

tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat     6300

gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat     6360

tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt     6420

aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag     6480

cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa     6540

agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg     6600

ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct     6660

tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac     6720

tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca     6780

caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat     6840

accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact     6900

attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc     6960

ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga     7020

taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg     7080

taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg     7140

aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca     7200

agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta     7260

ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca     7320

ctgagcgtca gacccc                                                     7336


<210>  14
<211>  8483
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW1020 - Adeno-associated virus (AAV) plasmids including
       a promoter, a transgene encoding two proteins separated by a 
       self-cleaving viral 2A peptide (a polyprotein), and a late SV40 
       polyadenylation signal

<400>  14
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg       60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact      120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg      180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg      240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac      300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca      360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga      420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc      480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct      540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg      600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct      660

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc      720

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc      780

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat      840

taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg      900

cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact      960

ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtggg     1020

aaattctaat ccccaccctg ctggaaactc tctggtggcc ccaacatgct aatcctccgg     1080

caaacctctg tttcctcctc aaaaggcagg aggtcggaaa gaataaacaa tgagagtcac     1140

attaaaaaca caaaatccta cggaaatact gaagaatgag tctcagcact aaggaaaagc     1200

ctccagcagc tcctgctttc tgagggtgaa ggatagacgc tgtggctctg catgactcac     1260

tagcactcta tcacggccat attctggcag ggtcagtggc tccaactaac atttgtttgg     1320

tactttacag tttattaaat agatgtttat atggagaagc tctcatttct ttctcagaag     1380

agcctggcta ggaaggtgga tgaggcacca tattcatttt gcaggtgaaa ttcctgagat     1440

gtaaggagct gctgtgactt gctcaaggcc ttatatcaag taaacggtag cgctggggct     1500

tagacgcagg tgttctgatt tatagttcaa aacctctatc aatgagagag caatctcctg     1560

gtaatgtgat agatttccca acttaatgcc aacataccat aaacctccca ttctgctaat     1620

gcccagccta agttggggag accactccag attccaagat gtacagtttg ctttgctggg     1680

cctttttccc atgcctgcct ttactctgcc agagttatat tgctggggtt ttgaagaaga     1740

tcctattaaa taaaagaata agcagtatta ttaagtagcc ctgcatttca ggtttccttg     1800

agtggcaggc caggcctggc gtgaacgttc actgaaatca tggcctcttg gccaagattg     1860

atagcttgtg cctgtccctg agtcccagtc catcacgagc agctggtttc taagatgcta     1920

tttcccgtat aaagcatgag accgtgactt gccagcccca cagagccccg cccttgtcca     1980

tcactggcat ctggactcca gcctgggttg gggcaaagag ggaaatgaga tcatgtccta     2040

accctgatcc tcttgtccca cagatatcca gaaccctgac cctgccgtgt accagctgag     2100

agactctaaa tccagtgaca agtctgtctg cctatacgcg taatgaaaga ccccacctgt     2160

aggtttggca agctaggatc aaggttagga acagagagac agcagaatat gggccaaaca     2220

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagtt ggaacagcag     2280

aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa     2340

cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc     2400

cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg     2460

cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc     2520

tcactcggcg cgattcacct gacgcgtctc gagggccgcc accatggccc tccctgtgac     2580

cgccctgctg ctccccctcg ccctgttgct ccatgctgcc cgacctggat ccatcctttg     2640

gcacgagatg tggcacgagg gactcgaaga agcgtcccgg ctgtacttcg gagagcggaa     2700

cgtgaagggg atgttcgaag tgctggaacc cctgcacgcc atgatggagc ggggtcctca     2760

gacccttaaa gaaacaagct tcaaccaggc gtacgggcgc gacctgatgg aagcccagga     2820

gtggtgccgc aagtacatga agtccggaaa cgtgaaggat ctgacccaag cctgggatct     2880

gtactaccac gtgttcagaa ggatctcaaa ggctagcgcc ggcactggtt cggatatcta     2940

catttgggca ccgctcgccg gcacttgtgg agtgctgttg ctgtccctcg tgatcaccat     3000

gcataagagg ggacggaaga agctgctgta cattttcaag cagccattca tgcggcctgt     3060

gcaaaccacc caggaggagg acgggtgcag ctgccggttc cctgaggaag aggagggcgg     3120

atgcgaactg cgcgtgaagt tcagccggag cgcagatgct cccgcatacc aacagggaca     3180

gaaccagctg tataacgagc tgaacctggg cagaagggaa gagtacgacg tcctcgacaa     3240

gcggcgggga cgcgacccag aaatgggagg aaagccccgc cggaagaacc cgcaggaagg     3300

cctgtacaac gagttgcaga aagacaagat ggctgaagct tactcggaga ttggcatgaa     3360

gggggagaga agaagaggga agggccacga cggcctttac caaggactga gcactgccac     3420

caaggacacc tacgatgcgc tgcacatgca ggccctgccc ccgcggtccg gttcgggcgc     3480

gactaacttc agcctgctga agcaggccgg agatgtggag gaaaaccctg gaccgtccat     3540

ggagactgat accctgcttc tgtgggtcct gctcctctgg gtgccgggct ccaccggtga     3600

catccagatg acccagacca cctcatccct gagcgcctct ctgggtgatc gcgtgactat     3660

ctcctgccgg gcgtcgcagg atatctccaa gtacctgaac tggtaccagc aaaaaccgga     3720

cgggaccgtg aaactgctga tctaccatac ttcccgcctt cattccggag tgccctcccg     3780

gttttccggc tcgggttcag ggactgatta ttcgctgacc atttccaacc tggagcagga     3840

ggacattgcg acctacttct gccaacaagg aaacaccctg ccctacactt tcggtggtgg     3900

aaccaagctc gagatcacag gtggcggtgg ctccggcggt ggtgggtctg gtggcggcgg     3960

aagcgaggtc aagctgcagg aatccggccc gggactggtg gccccgagcc agtcgctctc     4020

cgtcacttgc accgtgtcgg gagtgtcctt gcccgactac ggagtgtcat ggattcggca     4080

gccacctcgc aagggcctgg aatggctcgg cgtgatttgg ggctcagaaa ccacatacta     4140

caacagcgcc ctgaagtctc ggctcaccat catcaaggac aattccaagt cccaagtgtt     4200

cctgaagatg aatagcttgc agactgacga caccgcgatc tactactgtg ccaagcacta     4260

ctactacggc ggttcctacg ccatggacta ctggggacaa ggaacttccg tgactgtctc     4320

ctcccctagg gggggtggtg gttcgggggt ccaggtggaa accatttccc ccggcgacgg     4380

gcgcaccttc ccgaagcgcg gacagacctg tgtggtgcac tataccggaa tgctcgaaga     4440

tggaaagaag tttgacagct ccagggaccg caacaagcct ttcaagttta tgcttggaaa     4500

gcaggaagtc atccggggct gggaagaggg agtcgcccag atgagcgtcg gccagcgggc     4560

caagctgacg atctcccctg actatgccta cggcgctacc ggccatcccg gaatcattcc     4620

gccgcacgca accctcgtgt tcgacgtgga attgctcaag ctggaaggcg gccgcatggc     4680

gctgatagtg ctcggcggag tggccggact gctgctgttc atcggcctgg gcatcttctt     4740

ctgcgtgaga tgccgccata gaaggcggca atgagcggcc gcgctttatt tgtgaaattt     4800

gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca     4860

attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagctcac     4920

cggttttgat tctcaaacaa atgtgtcaca aagtaaggat tctgatgtgt atatcacaga     4980

caaaactgtg ctagacatga ggtctatgga cttcaagagc aacagtgctg tggcctggag     5040

caacaaatct gactttgcat gtgcaaacgc cttcaacaac agcattattc cagaagacac     5100

cttcttcccc agcccaggta agggcagctt tggtgccttc gcaggctgtt tccttgcttc     5160

aggaatggcc aggttctgcc cagagctctg gtcaatgatg tctaaaactc ctctgattgg     5220

tggtctcggc cttatccatt gccaccaaaa ccctcttttt actaagaaac agtgagcctt     5280

gttctggcag tccagagaat gacacgggaa aaaagcagat gaagagaagg tggcaggaga     5340

gggcacgtgg cccagcctca gtctctccaa ctgagttcct gcctgcctgc ctttgctcag     5400

actgtttgcc ccttactgct cttctaggcc tcattctaag ccccttctcc aagttgcctc     5460

tccttatttc tccctgtctg ccaaaaaatc tttcccagct cactaagtca gtctcacgca     5520

gtcactcatt aacccaccaa tcactgattg tgccggcaca tgaatgcacc aggtagataa     5580

gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc     5640

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg     5700

ctttgcccgg gcggcctcag tgagcgagcg agcgcgccag ctggcgtaat agcgaagagg     5760

cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgattccgtt     5820

gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct     5880

tctactcagg caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg     5940

cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat     6000

tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct     6060

gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg     6120

tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc     6180

cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg     6240

ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg     6300

gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg     6360

atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt     6420

ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt     6480

gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt     6540

taacaaaata ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg     6600

gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt     6660

catcgattct cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagagac     6720

ctctcaaaaa tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat     6780

attgatggtg atttgactgt ctccggcctt tctcacccgt ttgaatcttt acctacacat     6840

tactcaggca ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa     6900

ataaaggctt ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta     6960

gctttatgct ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat     7020

ttattggatg ttggaatcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt     7080

cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc     7140

cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg     7200

cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat     7260

caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca     7320

tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc     7380

ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct     7440

gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg     7500

cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg     7560

tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc     7620

tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca     7680

cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac     7740

tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa     7800

agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg     7860

ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt     7920

ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg     7980

aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc     8040

gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga     8100

tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta     8160

ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc     8220

cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg     8280

atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt     8340

cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa     8400

ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt     8460

cgttccactg agcgtcagac ccc                                             8483


<210>  15
<211>  8089
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered plasmid pBW841

<400>  15
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatcatat gccagcctat ggtgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      600

gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      660

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      720

caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag      780

cagagctcgt ttagtgaacc gggtctctct ggttagacca gatctgagcc tgggagctct      840

ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag      900

tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt      960

cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc     1020

agaggagatc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg     1080

gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagtagg     1140

gtgcgagagc gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa     1200

ggccaggggg aaagaaacaa tataaactaa aacatatagt tagggcaagc agggagctag     1260

aacgattcgc agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg     1320

gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacaa     1380

tagcagtcct ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag     1440

ataagataga ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca     1500

caggaaacaa cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa     1560

tggtacatca ggccatatca cctagaactt taaattaaga cagcagtaca aatggcagta     1620

ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata     1680

gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt     1740

caaaattttc gggtttatta cagggacagc agagatccag tttggaaagg accagcaaag     1800

ctcctctgga aaggtgaagg ggcagtagta atacaagata atagtgacat aaaagtagtg     1860

ccaagaagaa aagcaaagat catcagggat tatggaaaac agatggcagg tgatgattgt     1920

gtggcaagta gacaggatga ggattaacac atggaaaaga ttagtaaaac accatagctc     1980

tagagcgatc ccgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga     2040

attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa     2100

gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt     2160

cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag     2220

acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca     2280

acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc     2340

tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact     2400

catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat     2460

ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttggt     2520

aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc     2580

accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat     2640

agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatccat     2700

ctcgacggaa tgaaagaccc cacctgtagg tttggcaagc taggatcaag gttaggaaca     2760

gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct     2820

cagggccaag aacagttgga acagcagaat atgggccaaa caggatatct gtggtaagca     2880

gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc     2940

agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg     3000

ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc     3060

gagctcaata aaagagccca caacccctca ctcggcgcga ttcacctgac gcgtctcgag     3120

ggccgccacc atggccctcc ctgtgaccgc cctgctgctc cccctcgccc tgttgctcca     3180

tgctgcccga cctggatcca tcctttggca cgagatgtgg cacgagggac tcgaagaagc     3240

gtcccggctg tacttcggag agcggaacgt gaaggggatg ttcgaagtgc tggaacccct     3300

gcacgccatg atggagcggg gtcctcagac ccttaaagaa acaagcttca accaggcgta     3360

cgggcgcgac ctgatggaag cccaggagtg gtgccgcaag tacatgaagt ccggaaacgt     3420

gaaggatctg acccaagcct gggatctgta ctaccacgtg ttcagaagga tctcaaaggc     3480

tagcgccggc actggttcgg atatctacat ttgggcaccg ctcgccggca cttgtggagt     3540

gctgttgctg tccctcgtga tcaccatgca taagagggga cggaagaagc tgctgtacat     3600

tttcaagcag ccattcatgc ggcctgtgca aaccacccag gaggaggacg ggtgcagctg     3660

ccggttccct gaggaagagg agggcggatg cgaactgcgc gtgaagttca gccggagcgc     3720

agatgctccc gcataccaac agggacagaa ccagctgtat aacgagctga acctgggcag     3780

aagggaagag tacgacgtcc tcgacaagcg gcggggacgc gacccagaaa tgggaggaaa     3840

gccccgccgg aagaacccgc aggaaggcct gtacaacgag ttgcagaaag acaagatggc     3900

tgaagcttac tcggagattg gcatgaaggg ggagagaaga agagggaagg gccacgacgg     3960

cctttaccaa ggactgagca ctgccaccaa ggacacctac gatgcgctgc acatgcaggc     4020

cctgcccccg cggtccggtt cgggcgcgac taacttcagc ctgctgaagc aggccggaga     4080

tgtggaggaa aaccctggac cgtccatgga gactgatacc ctgcttctgt gggtcctgct     4140

cctctgggtg ccgggctcca ccggtgacat ccagatgacc cagaccacct catccctgag     4200

cgcctctctg ggtgatcgcg tgactatctc ctgccgggcg tcgcaggata tctccaagta     4260

cctgaactgg taccagcaaa aaccggacgg gaccgtgaaa ctgctgatct accatacttc     4320

ccgccttcat tccggagtgc cctcccggtt ttccggctcg ggttcaggga ctgattattc     4380

gctgaccatt tccaacctgg agcaggagga cattgcgacc tacttctgcc aacaaggaaa     4440

caccctgccc tacactttcg gtggtggaac caagctcgag atcacaggtg gcggtggctc     4500

cggcggtggt gggtctggtg gcggcggaag cgaggtcaag ctgcaggaat ccggcccggg     4560

actggtggcc ccgagccagt cgctctccgt cacttgcacc gtgtcgggag tgtccttgcc     4620

cgactacgga gtgtcatgga ttcggcagcc acctcgcaag ggcctggaat ggctcggcgt     4680

gatttggggc tcagaaacca catactacaa cagcgccctg aagtctcggc tcaccatcat     4740

caaggacaat tccaagtccc aagtgttcct gaagatgaat agcttgcaga ctgacgacac     4800

cgcgatctac tactgtgcca agcactacta ctacggcggt tcctacgcca tggactactg     4860

gggacaagga acttccgtga ctgtctcctc ccctaggggg ggtggtggtt cgggggtcca     4920

ggtggaaacc atttcccccg gcgacgggcg caccttcccg aagcgcggac agacctgtgt     4980

ggtgcactat accggaatgc tcgaagatgg aaagaagttt gacagctcca gggaccgcaa     5040

caagcctttc aagtttatgc ttggaaagca ggaagtcatc cggggctggg aagagggagt     5100

cgcccagatg agcgtcggcc agcgggccaa gctgacgatc tcccctgact atgcctacgg     5160

cgctaccggc catcccggaa tcattccgcc gcacgcaacc ctcgtgttcg acgtggaatt     5220

gctcaagctg gaaggcggcc gcatggcgct gatagtgctc ggcggagtgg ccggactgct     5280

gctgttcatc ggcctgggca tcttcttctg cgtgagatgc cgccatagaa ggcggcaatg     5340

agtcgactcg agtaccttta agaccaatga cttacaaggc agctgtagat cttagccact     5400

ttttaaaaga aaagggggga ctggaagggc taattcactc ccaaagaaga caagatctgc     5460

tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct     5520

aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caatgtgtgt     5580

gttggttttt tgtgtgtcga aattctagcg attctagctt ggcgtaatca tggtcatagc     5640

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca     5700

taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct     5760

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac     5820

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc     5880

tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt     5940

tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg     6000

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg     6060

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat     6120

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     6180

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     6240

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     6300

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa     6360

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg     6420

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag     6480

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt     6540

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta     6600

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc     6660

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca     6720

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa     6780

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat     6840

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct     6900

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt     6960

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat     7020

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta     7080

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg     7140

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt     7200

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg     7260

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg     7320

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc     7380

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa     7440

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac     7500

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt     7560

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg     7620

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa     7680

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata     7740

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggact agctttttgc     7800

aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg     7860

cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg     7920

aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact     7980

aattgagatg agcttgcatg ccgacattga ttattgacta gtccctaaga aaccattctt     8040

atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc                 8089


<210>  16
<211>  8619
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered plasmid pBW400

<400>  16
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca     3900

gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg     3960

cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag     4020

ggagtggcca actccatcac taggggttcc tgcggccgct acggctgggg cttagacgca     4080

ggtgttctga tttatagttc aaaacctcta tcaatgagag agcaatctcc tggtaatgtg     4140

atagatttcc caacttaatg ccaacatacc ataaacctcc cattctgcta atgcccagcc     4200

taagttgggg agaccactcc agattccaag atgtacagtt tgctttgctg ggcctttttc     4260

ccatgcctgc ctttactctg ccagagttat attgctgggg ttttgaagaa gatcctatta     4320

aataaaagaa taagcagtat tattaagtag ccctgcattt caggtttcct tgagtggcag     4380

gccaggcctg gcgtgaacgt tcactgaaat catggcctct tggccaagat tgatagcttg     4440

tgcctgtccc tgagtcccag tccatcacga gcagctggtt tctaagatgc tatttcccgt     4500

ataaagcatg agaccgtgac ttgccagccc cacagagccc cgcccttgtc catcactggc     4560

atctggactc cagcctgggt tggggcaaag agggaaatga gatcatgtcc taaccctgat     4620

cctcttgtcc cacagatatc cagaaccctg accctgccgt gtaccagctg agagactcta     4680

aatccagtga caagtctgtc tgcctatacg cgtgatccat cgattagtcc aatttgttaa     4740

agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc     4800

tatagagtac gagccataga tagaataaaa gattttattt agtctccaga aaaagggggg     4860

aatgaaagac cccacctgta ggtttggcaa gctaggatca aggttaggaa cagagagaca     4920

gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     4980

agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc     5040

cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta     5100

gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt     5160

gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa     5220

taaaagagcc cacaacccct cactcggcgc gacgcgtcat agccaccatg gccttaccag     5280

tgaccgcctt gctcctgccg ctggccttgc tgctccacgc cgccaggccg gacatccaga     5340

tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc atcagttgca     5400

gggcaagtca ggacattagt aaatatttaa attggtatca gcagaaacca gatggaactg     5460

ttaaactcct gatctaccat acatcaagat tacactcagg agtcccatca aggttcagtg     5520

gcagtgggtc tggaacagat tattctctca ccattagcaa cctggagcaa gaagatattg     5580

ccacttactt ttgccaacag ggtaatacgc ttccgtacac gttcggaggg gggaccaagc     5640

tggagatcac aggtggcggt ggctccggcg gtggtgggtc tggtggcggc ggaagcgagg     5700

tgaaactgca ggagtcagga cctggcctgg tggcgccctc acagagcctg tccgtcacat     5760

gcactgtctc aggggtctca ttacccgact atggtgtaag ctggattcgc cagcctccac     5820

gaaagggtct ggagtggctg ggagtaatat ggggtagtga aaccacatac tataattcag     5880

ctctcaaatc cagactgacc atcatcaagg acaactccaa gagccaagtt ttcttaaaaa     5940

tgaacagtct gcaaactgat gacacagcca tttactactg tgccaaacat tattactacg     6000

gtggtagcta tgctatggac tactggggtc aaggaacctc ggtcaccgtc tcctcaacca     6060

cgacgccagc gccgcgacca ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc     6120

tgcgcccaga ggcgtgccgg ccagcggcgg ggggcgcagt gcacacgagg gggctggact     6180

tcgcctgtga tatctacatc tgggcgccct tggccgggac ttgtggggtc cttctcctgt     6240

cactggtgat caccctttac tgcaaacggg gcagaaagaa actcctgtat atattcaaac     6300

aaccatttat gagaccagta caaactactc aagaggaaga tggctgtagc tgccgatttc     6360

cagaagaaga agaaggagga tgtgaactga gagtgaagtt cagcaggagc gcagacgccc     6420

ccgcgtacca gcagggccag aaccagctct ataacgagct caatctagga cgaagagagg     6480

agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga aagccgagaa     6540

ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg gcggaggcct     6600

acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat ggcctttacc     6660

agggtctcag tacagccacc aaggacacct acgacgccct tcacatgcag gccctgcccc     6720

ctcgctaagc ggccgctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta     6780

ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc     6840

atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc     6900

ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt     6960

tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg     7020

taaccattct agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat     7080

aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg     7140

ggagatgtgg gaggtttttt aaagctcacc ggttttgatt ctcaaacaaa tgtgtcacaa     7200

agtaaggatt ctgatgtgta tatcacagac aaaactgtgc tagacatgag gtctatggac     7260

ttcaagagca acagtgctgt ggcctggagc aacaaatctg actttgcatg tgcaaacgcc     7320

ttcaacaaca gcattattcc agaagacacc ttcttcccca gcccaggtaa gggcagcttt     7380

ggtgccttcg caggctgttt ccttgcttca ggaatggcca ggttctgccc agagctctgg     7440

tcaatgatgt ctaaaactcc tctgattggt ggtctcggcc ttatccattg ccaccaaaac     7500

cctcttttta ctaagaaaca gtgagccttg ttctggcagt ccagagaatg acacgggaaa     7560

aaagcagatg aagagaaggt ggcaggagag ggcacgtggc ccagcctcag tctctccaac     7620

tgagttcctg cctgcctgcc tttgctcaga ctgtttgccc cttactgctc ttctaggcct     7680

cattctaagc cccttctcca agttgcctct ccttatttct ccctgtctgc caaaaaatct     7740

ttcccagctc actaagtcag tctcacgcag tcactcatta acccaccaat cactgattgt     7800

gccggcacat gaatgcacca ggtagcggcc gcaggaaccc ctagtgatgg agttggccac     7860

tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc     7920

gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggaagctgt     7980

aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta     8040

cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt     8100

gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca     8160

aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc     8220

aatgtatctt atcatgtctg gatctgatca ctgatatcgc ctaggagatc cgaaccagat     8280

aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc     8340

tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt     8400

ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt     8460

atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt     8520

ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga     8580

ctgaatatca acgcttattt gcagcctgaa tggcgaatg                            8619


<210>  17
<211>  8457
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW1057 - Adeno-associated virus (AAV) plasmids 
       containing a promoter, a transgene encoding an intron-containing 
       chimeric antigen receptor (CAR) and a polyadenylation signal

<400>  17
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca     3900

gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg     3960

cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag     4020

ggagtggcca actccatcac taggggttcc tgcggccgct acggctgggg cttagacgca     4080

ggtgttctga tttatagttc aaaacctcta tcaatgagag agcaatctcc tggtaatgtg     4140

atagatttcc caacttaatg ccaacatacc ataaacctcc cattctgcta atgcccagcc     4200

taagttgggg agaccactcc agattccaag atgtacagtt tgctttgctg ggcctttttc     4260

ccatgcctgc ctttactctg ccagagttat attgctgggg ttttgaagaa gatcctatta     4320

aataaaagaa taagcagtat tattaagtag ccctgcattt caggtttcct tgagtggcag     4380

gccaggcctg gcgtgaacgt tcactgaaat catggcctct tggccaagat tgatagcttg     4440

tgcctgtccc tgagtcccag tccatcacga gcagctggtt tctaagatgc tatttcccgt     4500

ataaagcatg agaccgtgac ttgccagccc cacagagccc cgcccttgtc catcactggc     4560

atctggactc cagcctgggt tggggcaaag agggaaatga gatcatgtcc taaccctgat     4620

cctcttgtcc cacagatatc cagaaccctg accctgccgt gtaccagctg agagactcta     4680

aatccagtga caagtctgtc tgcctatacg cgtgatccat cgattagtcc aatttgttaa     4740

agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc     4800

tatagagtac gagccataga tagaataaaa gattttattt agtctccaga aaaagggggg     4860

aatgaaagac cccacctgta ggtttggcaa gctaggatca aggttaggaa cagagagaca     4920

gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     4980

agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc     5040

cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta     5100

gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt     5160

gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa     5220

taaaagagcc cacaacccct cactcggcgc ggtaagtatc aaggttacaa gacaggttta     5280

aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc     5340

acctattggt cttactgaca tccactttgc ctttctctcc acagacgcgt catagccacc     5400

atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg     5460

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc     5520

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa     5580

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca     5640

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag     5700

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga     5760

ggggggacca agctggagat cacaggtggc ggtggctccg gcggtggtgg gtctggtggc     5820

ggcggaagcg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc     5880

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt     5940

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca     6000

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa     6060

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa     6120

cattattact acggtggtag ctatgctatg gactactggg gtcaaggaac ctcggtcacc     6180

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg     6240

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg     6300

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg     6360

gtccttctcc tgtcactggt gatcaccctt tactgcaaac ggggcagaaa gaaactcctg     6420

tatatattca aacaaccatt tatgagacca gtacaaacta ctcaagagga agatggctgt     6480

agctgccgat ttccagaaga agaagaagga ggatgtgaac tgagagtgaa gttcagcagg     6540

agcgcagacg cccccgcgta ccagcagggc cagaaccagc tctataacga gctcaatcta     6600

ggacgaagag aggagtacga tgttttggac aagagacgtg gccgggaccc tgagatgggg     6660

ggaaagccga gaaggaagaa ccctcaggaa ggcctgtaca atgaactgca gaaagataag     6720

atggcggagg cctacagtga gattgggatg aaaggcgagc gccggagggg caaggggcac     6780

gatggccttt accagggtct cagtacagcc accaaggaca cctacgacgc ccttcacatg     6840

caggccctgc cccctcgcta agcggccgcg ctttatttgt gaaatttgtg atgctattgc     6900

tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt     6960

tatgtttcag gttcaggggg agatgtggga ggttttttaa agctcaccgg ttttgattct     7020

caaacaaatg tgtcacaaag taaggattct gatgtgtata tcacagacaa aactgtgcta     7080

gacatgaggt ctatggactt caagagcaac agtgctgtgg cctggagcaa caaatctgac     7140

tttgcatgtg caaacgcctt caacaacagc attattccag aagacacctt cttccccagc     7200

ccaggtaagg gcagctttgg tgccttcgca ggctgtttcc ttgcttcagg aatggccagg     7260

ttctgcccag agctctggtc aatgatgtct aaaactcctc tgattggtgg tctcggcctt     7320

atccattgcc accaaaaccc tctttttact aagaaacagt gagccttgtt ctggcagtcc     7380

agagaatgac acgggaaaaa agcagatgaa gagaaggtgg caggagaggg cacgtggccc     7440

agcctcagtc tctccaactg agttcctgcc tgcctgcctt tgctcagact gtttgcccct     7500

tactgctctt ctaggcctca ttctaagccc cttctccaag ttgcctctcc ttatttctcc     7560

ctgtctgcca aaaaatcttt cccagctcac taagtcagtc tcacgcagtc actcattaac     7620

ccaccaatca ctgattgtgc cggcacatga atgcaccagg tagcggccgc aggaacccct     7680

agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc     7740

aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag     7800

ctgcctgcag gaagctgtaa gcttgtcgag aagtactaga ggatcataat cagccatacc     7860

acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa     7920

cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa     7980

taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt     8040

ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gatatcgcct     8100

aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt     8160

cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa     8220

tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg     8280

ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc     8340

gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt     8400

attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatg        8457


<210>  18
<211>  8516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW1058 - Adeno-associated virus (AAV) plasmids 
       containing a promoter, a transgene encoding an intron-containing 
       chimeric antigen receptor (CAR) and a polyadenylation signal

<400>  18
gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc       60

gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc      120

acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt      180

agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg      240

ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt      300

ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta      360

taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt      420

aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat      480

gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      540

agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      600

catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      660

ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      720

atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      780

ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc      840

gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      900

ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      960

ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag     1020

gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa     1080

ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg     1140

gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa     1200

ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg     1260

gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt     1320

gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt     1380

caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag     1440

cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     1500

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     1560

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     1620

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     1680

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     1740

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     1800

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     1860

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     1920

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     1980

tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg     2040

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     2100

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     2160

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     2220

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     2280

ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc     2340

cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg     2400

cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct     2460

ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga     2520

caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag     2580

acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt     2640

tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga     2700

ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac     2760

aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg     2820

tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg     2880

ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca     2940

tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact     3000

gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc     3060

gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta     3120

cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct     3180

ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg     3240

agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg     3300

ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca     3360

tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa     3420

acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa     3480

ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca     3540

ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac     3600

cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc     3660

ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg     3720

cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt     3780

ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt     3840

gttcgcccag gactctagct atagttctag tggttggcta cattattgaa gcatttatca     3900

gggttattgt ctcagagcat gcctgcaggc agctgcgcgc tcgctcgctc actgaggccg     3960

cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag     4020

ggagtggcca actccatcac taggggttcc tgcggccgct acggctgggg cttagacgca     4080

ggtgttctga tttatagttc aaaacctcta tcaatgagag agcaatctcc tggtaatgtg     4140

atagatttcc caacttaatg ccaacatacc ataaacctcc cattctgcta atgcccagcc     4200

taagttgggg agaccactcc agattccaag atgtacagtt tgctttgctg ggcctttttc     4260

ccatgcctgc ctttactctg ccagagttat attgctgggg ttttgaagaa gatcctatta     4320

aataaaagaa taagcagtat tattaagtag ccctgcattt caggtttcct tgagtggcag     4380

gccaggcctg gcgtgaacgt tcactgaaat catggcctct tggccaagat tgatagcttg     4440

tgcctgtccc tgagtcccag tccatcacga gcagctggtt tctaagatgc tatttcccgt     4500

ataaagcatg agaccgtgac ttgccagccc cacagagccc cgcccttgtc catcactggc     4560

atctggactc cagcctgggt tggggcaaag agggaaatga gatcatgtcc taaccctgat     4620

cctcttgtcc cacagatatc cagaaccctg accctgccgt gtaccagctg agagactcta     4680

aatccagtga caagtctgtc tgcctatacg cgtgatccat cgattagtcc aatttgttaa     4740

agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc     4800

tatagagtac gagccataga tagaataaaa gattttattt agtctccaga aaaagggggg     4860

aatgaaagac cccacctgta ggtttggcaa gctaggatca aggttaggaa cagagagaca     4920

gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     4980

agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc     5040

cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta     5100

gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt     5160

gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa     5220

taaaagagcc cacaacccct cactcggcgc gacgcgtcat agccaccatg gccttaccag     5280

tgaccgcctt gctcctgccg ctggccttgc tgctccacgc cgccaggccg gacatccaga     5340

tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc atcagttgca     5400

gggcaagtca ggacattagt aaatatttaa attggtatca gcagaaacca gatggaactg     5460

ttaaactcct gatctaccat acatcaagat tacactcagg agtcccatca aggttcagtg     5520

gcagtgggtc tggaacagat tattctctca ccattagcaa cctggagcaa gaagatattg     5580

ccacttactt ttgccaacag ggtaatacgc ttccgtacac gttcggaggg gggaccaagc     5640

tggagatcac aggtggcggt ggctccggcg gtggtgggtc tggtggcggc ggaagcgagg     5700

tgaaactgca ggagtcagga cctggcctgg tggcgccctc acagagcctg tccgtcacat     5760

gcactgtctc aggggtctca ttacccgact atggtgtaag ctggattcgc cagcctccac     5820

gaaagggtct ggagtggctg ggagtaatat ggggtagtga aaccacatac tataattcag     5880

ctctcaaatc cagactgacc atcatcaagg acaactccaa gagccaagtt ttcttaaaaa     5940

tgaacagtct gcaaactgat gacacagcca tttactactg tgccaaacat tattactacg     6000

gtggtagcta tgctatggac tactggggtc aaggaacctc gtaagaacca aaccctccca     6060

gcaggggtgc ccaggcccag gcatggccca gagggagcag cgggtggggc ttaggccaag     6120

ctgagctcac accttgacct ttcattccag ggtcaccgtc tcctcaacca cgacgccagc     6180

gccgcgacca ccaacaccgg cgcccaccat cgcgtcgcag cccctgtccc tgcgcccaga     6240

ggcgtgccgg ccagcggcgg ggggcgcagt gcacacgagg gggctggact tcgcctgtga     6300

tatctacatc tgggcgccct tggccgggac ttgtggggtc cttctcctgt cactggtgat     6360

caccctttac tgcaaacgtg agtacaggag gtggagagtg gccagccctt ctcatgttca     6420

gagaacatgg ttaactggtt aagtcatgtc gtcccacagg gggcagaaag aaactcctgt     6480

atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa gatggctgta     6540

gctgccgatt tccagaagaa gaagaaggag gatgtgaact gagagtgaag ttcagcagga     6600

gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataacgag ctcaatctag     6660

gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct gagatggggg     6720

gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag aaagataaga     6780

tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc aaggggcacg     6840

atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc cttcacatgc     6900

aggccctgcc ccctcgctaa gcggccgcgc tttatttgtg aaatttgtga tgctattgct     6960

ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt     7020

atgtttcagg ttcaggggga gatgtgggag gttttttaaa gctcaccggt tttgattctc     7080

aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa actgtgctag     7140

acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac aaatctgact     7200

ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc ttccccagcc     7260

caggtaaggg cagctttggt gccttcgcag gctgtttcct tgcttcagga atggccaggt     7320

tctgcccaga gctctggtca atgatgtcta aaactcctct gattggtggt ctcggcctta     7380

tccattgcca ccaaaaccct ctttttacta agaaacagtg agccttgttc tggcagtcca     7440

gagaatgaca cgggaaaaaa gcagatgaag agaaggtggc aggagagggc acgtggccca     7500

gcctcagtct ctccaactga gttcctgcct gcctgccttt gctcagactg tttgcccctt     7560

actgctcttc taggcctcat tctaagcccc ttctccaagt tgcctctcct tatttctccc     7620

tgtctgccaa aaaatctttc ccagctcact aagtcagtct cacgcagtca ctcattaacc     7680

caccaatcac tgattgtgcc ggcacatgaa tgcaccaggt agcggccgca ggaaccccta     7740

gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca     7800

aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc     7860

tgcctgcagg aagctgtaag cttgtcgaga agtactagag gatcataatc agccatacca     7920

catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac     7980

ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat     8040

aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg     8100

gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta     8160

ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc     8220

gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat     8280

ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg     8340

gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg     8400

tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta     8460

ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatg         8516


<210>  19
<211>  8169
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered plasmid pBW1059

<400>  19
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg       60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact      120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg      180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg      240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac      300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca      360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga      420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc      480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct      540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg      600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct      660

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc      720

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc      780

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat      840

taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg      900

cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact      960

ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctacgctgg     1020

ggcttagacg caggtgttct gatttatagt tcaaaacctc tatcaatgag agagcaatct     1080

cctggtaatg tgatagattt cccaacttaa tgccaacata ccataaacct cccattctgc     1140

taatgcccag cctaagttgg ggagaccact ccagattcca agatgtacag tttgctttgc     1200

tgggcctttt tcccatgcct gcctttactc tgccagagtt atattgctgg ggttttgaag     1260

aagatcctat taaataaaag aataagcagt attattaagt agccctgcat ttcaggtttc     1320

cttgagtggc aggccaggcc tggcgtgaac gttcactgaa atcatggcct cttggccaag     1380

attgatagct tgtgcctgtc cctgagtccc agtccatcac gagcagctgg tttctaagat     1440

gctatttccc gtataaagca tgagaccgtg acttgccagc cccacagagc cccgcccttg     1500

tccatcactg gcatctggac tccagcctgg gttggggcaa agagggaaat gagatcatgt     1560

cctaaccctg atcctcttgt cccacagata tccagaaccc tgaccctgcc gtgtaccagc     1620

tgagagactc taaatccagt gacaagtctg tctgcctatc atatgcacac aaaaaaccaa     1680

cacacagatg tctagtagct ctgatctttt attctagcgg ccgcgtagcg ctgctatcag     1740

gagctcagct tctgctgcct gttcacgcgg tagcagtaga agatgatgat cacgctgatc     1800

gccaccccca ggggcggcag gagggaaatg ccagtgactt ggaagatcac gagcagcaga     1860

tcagggttcg aggtattgta ctcctcggaa aagataatgt tgtcgttgca ttcgtcggat     1920

gagcaggaac acatgaagaa cgtttcgccc ggcttctttt tctccttcat gatgcacttg     1980

gggcttgcgg cgtcctccag aatgaagtcg tggtatggaa gcttaggatc gtggcacacg     2040

gtttccaggg tgatgttctc gtcatttttc cgccacacgg ccacacagac ttcctgaggc     2100

ttttcgcaga tagaggtaat ggagcagttg ctcatgcatg acttctggtt gtcgcaagtc     2160

gaaaagcgca cgtcacagaa cttgcagagc tgcgggaact tgacggcacc gttgttatcg     2220

gtcacgatca tgtcgttgtt caccgacttt tgcacgtgcg ggggaatggt ggaagcaatc     2280

cgagtccaaa gcacgatatg cagcggccac aatcctctca acagtcctct tcccatggtg     2340

gcacgcgcct tgctagctag acaaaagtgt tgtggaattg ctccaggcga tctgacggtt     2400

cactaaacga gctctgcttt tataggcgcc caccgtacac gcctagatcc atcgattagt     2460

ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca     2520

ccagctgaag cctatagagt acgagccata gatagaataa aagattttat ttagtctcca     2580

gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctaggat caaggttagg     2640

aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc     2700

ggctcagggc caagaacagt tggaacagca gaatatgggc caaacaggat atctgtggta     2760

agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct     2820

cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc     2880

tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct     2940

ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgacgcgtc atagccacca     3000

tggccttacc agtgaccgcc ttgctcctgc cgctggcctt gctgctccac gccgccaggc     3060

cggacatcca gatgacacag actacatcct ccctgtctgc ctctctggga gacagagtca     3120

ccatcagttg cagggcaagt caggacatta gtaaatattt aaattggtat cagcagaaac     3180

cagatggaac tgttaaactc ctgatctacc atacatcaag attacactca ggagtcccat     3240

caaggttcag tggcagtggg tctggaacag attattctct caccattagc aacctggagc     3300

aagaagatat tgccacttac ttttgccaac agggtaatac gcttccgtac acgttcggag     3360

gggggaccaa gctggagatc acaggtggcg gtggctccgg cggtggtggg tctggtggcg     3420

gcggaagcga ggtgaaactg caggagtcag gacctggcct ggtggcgccc tcacagagcc     3480

tgtccgtcac atgcactgtc tcaggggtct cattacccga ctatggtgta agctggattc     3540

gccagcctcc acgaaagggt ctggagtggc tgggagtaat atggggtagt gaaaccacat     3600

actataattc agctctcaaa tccagactga ccatcatcaa ggacaactcc aagagccaag     3660

ttttcttaaa aatgaacagt ctgcaaactg atgacacagc catttactac tgtgccaaac     3720

attattacta cggtggtagc tatgctatgg actactgggg tcaaggaacc tcggtcaccg     3780

tctcctcaac cacgacgcca gcgccgcgac caccaacacc ggcgcccacc atcgcgtcgc     3840

agcccctgtc cctgcgccca gaggcgtgcc ggccagcggc ggggggcgca gtgcacacga     3900

gggggctgga cttcgcctgt gatatctaca tctgggcgcc cttggccggg acttgtgggg     3960

tccttctcct gtcactggtg atcacccttt actgcaaacg gggcagaaag aaactcctgt     4020

atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa gatggctgta     4080

gctgccgatt tccagaagaa gaagaaggag gatgtgaact gagagtgaag ttcagcagga     4140

gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataacgag ctcaatctag     4200

gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct gagatggggg     4260

gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag aaagataaga     4320

tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc aaggggcacg     4380

atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc cttcacatgc     4440

aggccctgcc ccctcgctaa gcggccgcgc tttatttgtg aaatttgtga tgctattgct     4500

ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt     4560

atgtttcagg ttcaggggga gatgtgggag gttttttaaa gctcaccggt tttgattctc     4620

aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa actgtgctag     4680

acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac aaatctgact     4740

ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc ttccccagcc     4800

caggtaaggg cagctttggt gccttcgcag gctgtttcct tgcttcagga atggccaggt     4860

tctgcccaga gctctggtca atgatgtcta aaactcctct gattggtggt ctcggcctta     4920

tccattgcca ccaaaaccct ctttttacta agaaacagtg agccttgttc tggcagtcca     4980

gagaatgaca cgggaaaaaa gcagatgaag agaaggtggc aggagagggc acgtggccca     5040

gcctcagtct ctccaactga gttcctgcct gcctgccttt gctcagactg tttgcccctt     5100

actgctcttc taggcctcat tctaagcccc ttctccaagt tgcctctcct tatttctccc     5160

tgtctgccaa aaaatctttc ccagctcact aagtcagtct cacgcagtca ctcattaacc     5220

caccaatcac tgattgtgcc ggcacatgaa tgcaccaggt agataagtag catggcgggt     5280

taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc     5340

gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg     5400

cctcagtgag cgagcgagcg cgccagctgg cgtaatagcg aagaggcccg caccgatcgc     5460

ccttcccaac agttgcgcag cctgaatggc gaatggcgat tccgttgcaa tggctggcgg     5520

taatattgtt ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag     5580

tgatgttatt actaatcaaa gaagtattgc gacaacggtt aatttgcgtg atggacagac     5640

tcttttactc ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt     5700

cctgtctaaa atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga     5760

aagcacgtta tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa     5820

gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc     5880

ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag     5940

ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca     6000

aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc     6060

gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa     6120

cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct     6180

attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa     6240

cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat     6300

caaccggggt acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg     6360

tttgctccag actctcaggc aatgacctga tagcctttgt agagacctct caaaaatagc     6420

taccctctcc ggcatgaatt tatcagctag aacggttgaa tatcatattg atggtgattt     6480

gactgtctcc ggcctttctc acccgtttga atctttacct acacattact caggcattgc     6540

atttaaaata tatgagggtt ctaaaaattt ttatccttgc gttgaaataa aggcttctcc     6600

cgcaaaagta ttacagggtc ataatgtttt tggtacaacc gatttagctt tatgctctga     6660

ggctttattg cttaattttg ctaattcttt gccttgcctg tatgatttat tggatgttgg     6720

aatcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg     6780

tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca     6840

acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct     6900

gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg     6960

agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt     7020

tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt     7080

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa     7140

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt     7200

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat     7260

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag     7320

atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg     7380

ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata     7440

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat     7500

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc     7560

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg     7620

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac     7680

gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact     7740

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa     7800

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct     7860

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc     7920

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga     7980

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac     8040

tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag     8100

atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg     8160

tcagacccc                                                             8169


<210>  20
<211>  8058
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW1086 - An adeno-associated virus (AAV) plasmid 
       containing a viral self-cleaving peptide, e.g., T2A peptide, a 
       CD19-CAR transgene and a polyadenylation signal

<400>  20
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg       60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact      120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg      180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg      240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac      300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca      360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga      420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc      480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct      540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg      600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct      660

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc      720

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc      780

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat      840

taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg      900

cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact      960

ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctacgctgg     1020

ggcttagacg caggtgttct gatttatagt tcaaaacctc tatcaatgag agagcaatct     1080

cctggtaatg tgatagattt cccaacttaa tgccaacata ccataaacct cccattctgc     1140

taatgcccag cctaagttgg ggagaccact ccagattcca agatgtacag tttgctttgc     1200

tgggcctttt tcccatgcct gcctttactc tgccagagtt atattgctgg ggttttgaag     1260

aagatcctat taaataaaag aataagcagt attattaagt agccctgcat ttcaggtttc     1320

cttgagtggc aggccaggcc tggcgtgaac gttcactgaa atcatggcct cttggccaag     1380

attgatagct tgtgcctgtc cctgagtccc agtccatcac gagcagctgg tttctaagat     1440

gctatttccc gtataaagca tgagaccgtg acttgccagc cccacagagc cccgcccttg     1500

tccatcactg gcatctggac tccagcctgg gttggggcaa agagggaaat gagatcatgt     1560

cctaaccctg atcctcttgt cccacagata tccagaaccc tgaccctgcc gtgtaccagc     1620

tgagagactc taaatccagt gacaagtctg tctgcctata cgcgtgatcc atcgattagt     1680

ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca     1740

ccagctgaag cctatagagt acgagccata gatagaataa aagattttat ttagtctcca     1800

gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctaggat caaggttagg     1860

aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc     1920

ggctcagggc caagaacagt tggaacagca gaatatgggc caaacaggat atctgtggta     1980

agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct     2040

cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc     2100

tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct     2160

ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgacgcgtc atagccacca     2220

tggccttacc agtgaccgcc ttgctcctgc cgctggcctt gctgctccac gccgccaggc     2280

cggacatcca gatgacacag actacatcct ccctgtctgc ctctctggga gacagagtca     2340

ccatcagttg cagggcaagt caggacatta gtaaatattt aaattggtat cagcagaaac     2400

cagatggaac tgttaaactc ctgatctacc atacatcaag attacactca ggagtcccat     2460

caaggttcag tggcagtggg tctggaacag attattctct caccattagc aacctggagc     2520

aagaagatat tgccacttac ttttgccaac agggtaatac gcttccgtac acgttcggag     2580

gggggaccaa gctggagatc acaggtggcg gtggctccgg cggtggtggg tctggtggcg     2640

gcggaagcga ggtgaaactg caggagtcag gacctggcct ggtggcgccc tcacagagcc     2700

tgtccgtcac atgcactgtc tcaggggtct cattacccga ctatggtgta agctggattc     2760

gccagcctcc acgaaagggt ctggagtggc tgggagtaat atggggtagt gaaaccacat     2820

actataattc agctctcaaa tccagactga ccatcatcaa ggacaactcc aagagccaag     2880

ttttcttaaa aatgaacagt ctgcaaactg atgacacagc catttactac tgtgccaaac     2940

attattacta cggtggtagc tatgctatgg actactgggg tcaaggaacc tcggtcaccg     3000

tctcctcaac cacgacgcca gcgccgcgac caccaacacc ggcgcccacc atcgcgtcgc     3060

agcccctgtc cctgcgccca gaggcgtgcc ggccagcggc ggggggcgca gtgcacacga     3120

gggggctgga cttcgcctgt gatatctaca tctgggcgcc cttggccggg acttgtgggg     3180

tccttctcct gtcactggtg atcacccttt actgcaaacg gggcagaaag aaactcctgt     3240

atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa gatggctgta     3300

gctgccgatt tccagaagaa gaagaaggag gatgtgaact gagagtgaag ttcagcagga     3360

gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataacgag ctcaatctag     3420

gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct gagatggggg     3480

gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag aaagataaga     3540

tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc aaggggcacg     3600

atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc cttcacatgc     3660

aggccctgcc ccctcgctcc ggttcgggcg cgactaactt cagcctgctg aagcaggccg     3720

gagatgtgga ggaaaaccct ggaccgtcca tgggaagagg actgttgaga ggattgtggc     3780

cgctgcatat cgtgctttgg actcggattg cttccaccat tcccccgcac gtgcaaaagt     3840

cggtgaacaa cgacatgatc gtgaccgata acaacggtgc cgtcaagttc ccgcagctct     3900

gcaagttctg tgacgtgcgc ttttcgactt gcgacaacca gaagtcatgc atgagcaact     3960

gctccattac ctctatctgc gaaaagcctc aggaagtctg tgtggccgtg tggcggaaaa     4020

atgacgagaa catcaccctg gaaaccgtgt gccacgatcc taagcttcca taccacgact     4080

tcattctgga ggacgccgca agccccaagt gcatcatgaa ggagaaaaag aagccgggcg     4140

aaacgttctt catgtgttcc tgctcatccg acgaatgcaa cgacaacatt atcttttccg     4200

aggagtacaa tacctcgaac cctgatctgc tgctcgtgat cttccaagtc actggcattt     4260

ccctcctgcc gcccctgggg gtggcgatca gcgtgatcat catcttctac tgctaccgcg     4320

tgaacaggca gcagaagctg agctcctgag cggccgcgct ttatttgtga aatttgtgat     4380

gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc     4440

attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag ctcaccggtt     4500

ttgattctca aacaaatgtg tcacaaagta aggattctga tgtgtatatc acagacaaaa     4560

ctgtgctaga catgaggtct atggacttca agagcaacag tgctgtggcc tggagcaaca     4620

aatctgactt tgcatgtgca aacgccttca acaacagcat tattccagaa gacaccttct     4680

tccccagccc aggtaagggc agctttggtg ccttcgcagg ctgtttcctt gcttcaggaa     4740

tggccaggtt ctgcccagag ctctggtcaa tgatgtctaa aactcctctg attggtggtc     4800

tcggccttat ccattgccac caaaaccctc tttttactaa gaaacagtga gccttgttct     4860

ggcagtccag agaatgacac gggaaaaaag cagatgaaga gaaggtggca ggagagggca     4920

cgtggcccag cctcagtctc tccaactgag ttcctgcctg cctgcctttg ctcagactgt     4980

ttgcccctta ctgctcttct aggcctcatt ctaagcccct tctccaagtt gcctctcctt     5040

atttctccct gtctgccaaa aaatctttcc cagctcacta agtcagtctc acgcagtcac     5100

tcattaaccc accaatcact gattgtgccg gcacatgaat gcaccaggta gataagtagc     5160

atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc     5220

tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg     5280

cccgggcggc ctcagtgagc gagcgagcgc gccagctggc gtaatagcga agaggcccgc     5340

accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgatt ccgttgcaat     5400

ggctggcggt aatattgttc tggatattac cagcaaggcc gatagtttga gttcttctac     5460

tcaggcaagt gatgttatta ctaatcaaag aagtattgcg acaacggtta atttgcgtga     5520

tggacagact cttttactcg gtggcctcac tgattataaa aacacttctc aggattctgg     5580

cgtaccgttc ctgtctaaaa tccctttaat cggcctcctg tttagctccc gctctgattc     5640

taacgaggaa agcacgttat acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg     5700

gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg     5760

ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc     5820

cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc     5880

tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga     5940

cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa     6000

ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga     6060

tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca     6120

aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt     6180

tctgattatc aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg     6240

attctcttgt ttgctccaga ctctcaggca atgacctgat agcctttgta gagacctctc     6300

aaaaatagct accctctccg gcatgaattt atcagctaga acggttgaat atcatattga     6360

tggtgatttg actgtctccg gcctttctca cccgtttgaa tctttaccta cacattactc     6420

aggcattgca tttaaaatat atgagggttc taaaaatttt tatccttgcg ttgaaataaa     6480

ggcttctccc gcaaaagtat tacagggtca taatgttttt ggtacaaccg atttagcttt     6540

atgctctgag gctttattgc ttaattttgc taattctttg ccttgcctgt atgatttatt     6600

ggatgttgga atcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac     6660

cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga     6720

cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac     6780

agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg     6840

aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata     6900

ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt     6960

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa     7020

atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt     7080

attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa     7140

gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac     7200

agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt     7260

aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt     7320

cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat     7380

cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac     7440

actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg     7500

cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc     7560

ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa     7620

ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag     7680

gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct     7740

gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat     7800

ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa     7860

cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac     7920

caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc     7980

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     8040

cactgagcgt cagacccc                                                   8058


<210>  21
<211>  8035
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered plasmid pBW1087

<400>  21
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatcatat gccagcctat ggtgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca      600

gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat      660

tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa      720

caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag      780

cagagctcgt ttagtgaacc gggtctctct ggttagacca gatctgagcc tgggagctct      840

ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag      900

tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt      960

cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc     1020

agaggagatc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg     1080

gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagtagg     1140

gtgcgagagc gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa     1200

ggccaggggg aaagaaacaa tataaactaa aacatatagt tagggcaagc agggagctag     1260

aacgattcgc agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg     1320

gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacaa     1380

tagcagtcct ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag     1440

ataagataga ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca     1500

caggaaacaa cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa     1560

tggtacatca ggccatatca cctagaactt taaattaaga cagcagtaca aatggcagta     1620

ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata     1680

gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt     1740

caaaattttc gggtttatta cagggacagc agagatccag tttggaaagg accagcaaag     1800

ctcctctgga aaggtgaagg ggcagtagta atacaagata atagtgacat aaaagtagtg     1860

ccaagaagaa aagcaaagat catcagggat tatggaaaac agatggcagg tgatgattgt     1920

gtggcaagta gacaggatga ggattaacac atggaaaaga ttagtaaaac accatagctc     1980

tagagcgatc ccgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga     2040

attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa     2100

gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt     2160

cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag     2220

acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca     2280

acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc     2340

tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact     2400

catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat     2460

ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttggt     2520

aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc     2580

accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat     2640

agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatccat     2700

cgattagtcc aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa     2760

caatatcacc agctgaagcc tatagagtac gagccataga tagaataaaa gattttattt     2820

agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctaggatca     2880

aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt     2940

cctgccccgg ctcagggcca agaacagttg gaacagcaga atatgggcca aacaggatat     3000

ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt     3060

cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga     3120

aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg     3180

cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gacgcgtcat     3240

agccaccatg gccttaccag tgaccgcctt gctcctgccg ctggccttgc tgctccacgc     3300

cgccaggccg gacatccaga tgacacagac tacatcctcc ctgtctgcct ctctgggaga     3360

cagagtcacc atcagttgca gggcaagtca ggacattagt aaatatttaa attggtatca     3420

gcagaaacca gatggaactg ttaaactcct gatctaccat acatcaagat tacactcagg     3480

agtcccatca aggttcagtg gcagtgggtc tggaacagat tattctctca ccattagcaa     3540

cctggagcaa gaagatattg ccacttactt ttgccaacag ggtaatacgc ttccgtacac     3600

gttcggaggg gggaccaagc tggagatcac aggtggcggt ggctccggcg gtggtgggtc     3660

tggtggcggc ggaagcgagg tgaaactgca ggagtcagga cctggcctgg tggcgccctc     3720

acagagcctg tccgtcacat gcactgtctc aggggtctca ttacccgact atggtgtaag     3780

ctggattcgc cagcctccac gaaagggtct ggagtggctg ggagtaatat ggggtagtga     3840

aaccacatac tataattcag ctctcaaatc cagactgacc atcatcaagg acaactccaa     3900

gagccaagtt ttcttaaaaa tgaacagtct gcaaactgat gacacagcca tttactactg     3960

tgccaaacat tattactacg gtggtagcta tgctatggac tactggggtc aaggaacctc     4020

ggtcaccgtc tcctcaacca cgacgccagc gccgcgacca ccaacaccgg cgcccaccat     4080

cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg ccagcggcgg ggggcgcagt     4140

gcacacgagg gggctggact tcgcctgtga tatctacatc tgggcgccct tggccgggac     4200

ttgtggggtc cttctcctgt cactggtgat caccctttac tgcaaacggg gcagaaagaa     4260

actcctgtat atattcaaac aaccatttat gagaccagta caaactactc aagaggaaga     4320

tggctgtagc tgccgatttc cagaagaaga agaaggagga tgtgaactga gagtgaagtt     4380

cagcaggagc gcagacgccc ccgcgtacca gcagggccag aaccagctct ataacgagct     4440

caatctagga cgaagagagg agtacgatgt tttggacaag agacgtggcc gggaccctga     4500

gatgggggga aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa     4560

agataagatg gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa     4620

ggggcacgat ggcctttacc agggtctcag tacagccacc aaggacacct acgacgccct     4680

tcacatgcag gccctgcccc ctcgctaatg acaggtacct tatcgataat caacctctgg     4740

attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat     4800

gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt     4860

tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca     4920

ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg     4980

ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg     5040

aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca     5100

attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca     5160

cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc     5220

ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc     5280

agacgagtcg gatctccctt tgggccgcct ccccgcatcg aactgtacct ttaagaccaa     5340

tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag     5400

ggctaattca ctcccaaaga agacaagatc tgctttttgc ctgtactggg tctctctggt     5460

tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc     5520

aataaagctt gccttgagtg cttcaatgtg tgtgttggtt ttttgtgtgt cgaaattcta     5580

gcgattctag cttggcgtac cagcctatgg cgctcacaat tccacacaac atacgagccg     5640

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     5700

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     5760

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     5820

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     5880

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     5940

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     6000

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     6060

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     6120

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     6180

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     6240

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     6300

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     6360

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     6420

gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     6480

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     6540

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     6600

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     6660

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     6720

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     6780

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     6840

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     6900

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     6960

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     7020

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     7080

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     7140

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     7200

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     7260

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     7320

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     7380

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     7440

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     7500

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     7560

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     7620

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     7680

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggactagct     7740

ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg     7800

ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat     7860

gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg     7920

ctgactaatt gagatgagct tgcatgccga cattgattat tgactagtcc ctaagaaacc     7980

attcttatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc          8035


<210>  22
<211>  7625
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBW1088 - Adeno-associated virus (AAV) plasmids 
       containing a promoter, an alpha and a beta chain of a T cell 
       receptor specific for Wilms Tumor Antigen 1 (WT1-TCR), and a 
       polyadenylation signal

<400>  22
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg       60

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact      120

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg      180

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg      240

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac      300

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca      360

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga      420

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc      480

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct      540

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg      600

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct      660

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc      720

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc      780

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat      840

taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg      900

cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact      960

ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctacgctgg     1020

ggcttagacg caggtgttct gatttatagt tcaaaacctc tatcaatgag agagcaatct     1080

cctggtaatg tgatagattt cccaacttaa tgccaacata ccataaacct cccattctgc     1140

taatgcccag cctaagttgg ggagaccact ccagattcca agatgtacag tttgctttgc     1200

tgggcctttt tcccatgcct gcctttactc tgccagagtt atattgctgg ggttttgaag     1260

aagatcctat taaataaaag aataagcagt attattaagt agccctgcat ttcaggtttc     1320

cttgagtggc aggccaggcc tggcgtgaac gttcactgaa atcatggcct cttggccaag     1380

attgatagct tgtgcctgtc cctgagtccc agtccatcac gagcagctgg tttctaagat     1440

gctatttccc gtataaagca tgagaccgtg acttgccagc cccacagagc cccgcccttg     1500

tccatcactg gcatctggac tccagcctgg gttggggcaa agagggaaat gagatcatgt     1560

cctaaccctg atcctcttgt cccacagata tccagaaccc tgaccctgcc gtgtaccagc     1620

tgagagactc taaatccagt gacaagtctg tctgcctata cgcgtgaaca gagaaacagg     1680

agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag     1740

aacagttgga acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc     1800

cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga     1860

gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga     1920

actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctctata     1980

taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg     2040

acttccatag aaggatctcg agctacgcca ccatgctcct gctgcttgtc cctgtcctgg     2100

aagtcatatt cactctcggc ggaacccgcg cccaatcagt gacgcagctc gactcacacg     2160

tgtccgtgtc ggagggcacc cccgtgctgc tccggtgcaa ttactcctcc tcctactcgc     2220

cctccctgtt ttggtacgtc cagcatccga acaagggtct gcagttgctg ctgaagtaca     2280

ccagcgccgc aaccctcgtg aaaggcatta acggattcga agcggaattc aagaagtcgg     2340

aaaccagctt ccacctgact aagccttccg cgcacatgtc cgacgctgcc gagtatttct     2400

gcgtcgtgtc acccttctcc gggggtggag ccgacggact gaccttcggg aagggcaccc     2460

acctgatcat tcagccatac atccagaacc cggatcccgc ggtgtaccag ctgagagact     2520

cgaagtcttc cgataaatcc gtgtgtctct ttacagactt cgacagccag accaacgtgt     2580

cacagtccaa ggacagcgat gtgtacatca cggacaagac ggtgctggac atgcggtcta     2640

tggactttaa gtcgaacagc gctgtggcct ggagcaacaa gagcgacttc gcctgtgcga     2700

acgccttcaa caactccatc atcccggagg ataccttctt cccgtccccg gaaagctcct     2760

gcgacgtcaa gctcgtggaa aagagctttg aaaccgacac caacctgaac ttccaaaacc     2820

tgtccgtgat cggatttcgg attctgctgc tgaaagtggc cggattcaac cttctgatga     2880

ctctccggct gtggtcgagc cgggccaagc gcggatccgg cgcgaccaac ttctcactgc     2940

tgaaacaggc cggcgatgtg gaggagaacc ccggccctat gctgctgctc ctccttctgc     3000

tcggacccgg ctccggtctg ggtgccgtgg tgtcgcagca tccttcgtgg gtgatctgca     3060

agtcggggac ctccgtgaag atcgagtgcc gcagccttga cttccaagcc actactatgt     3120

tctggtatag gcagttcccg aagcagtcgc tgatgttgat ggccacttcc aacgaaggat     3180

caaaggctac ctacgagcag ggcgtcgaaa aggacaagtt cctgattaac catgcctccc     3240

tgactctgtc cacattgacc gtgactagcg cacatccaga ggactcctcg ttctacattt     3300

gctcggcccg ggacggcgga gagggctccg agactcagta cttcggaccg gggactaggc     3360

tcctggtgtt ggaggacctc aagaacgtgt tccctccgga agtggccgtg ttcgagccgt     3420

cagaggcgga gatctcgcac acccagaagg ctacccttgt gtgcctggcc accggattct     3480

accctgatca cgtggagctg tcttggtggg tcaacggaaa ggaagtgcac tcgggggtgt     3540

ccactgatcc acagcctctg aaggaacagc cggccctgaa cgactcccgg tattgcctga     3600

gcagcagact gcgcgtcagc gccaccttct ggcaaaatcc ccgaaaccac ttccgctgcc     3660

aagtccagtt ctacggactg tccgagaacg acgaatggac ccaggataga gccaagcctg     3720

tgacccaaat cgtgtccgcc gaagcatggg ggagggcgga ttgcggcttc acctcggaat     3780

cctaccaaca aggagtgctg tccgccacca tcctctacga gattctcctg ggaaaggcca     3840

ccctgtacgc cgtgttggtg tccgccctcg tgctgatggc aatggtcaag agaaaagact     3900

cccggggtta atgacagcgg ccgcgcttta tttgtgaaat ttgtgatgct attgctttat     3960

ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt     4020

ttcaggttca gggggagatg tgggaggttt tttaaagctc accggttttg attctcaaac     4080

aaatgtgtca caaagtaagg attctgatgt gtatatcaca gacaaaactg tgctagacat     4140

gaggtctatg gacttcaaga gcaacagtgc tgtggcctgg agcaacaaat ctgactttgc     4200

atgtgcaaac gccttcaaca acagcattat tccagaagac accttcttcc ccagcccagg     4260

taagggcagc tttggtgcct tcgcaggctg tttccttgct tcaggaatgg ccaggttctg     4320

cccagagctc tggtcaatga tgtctaaaac tcctctgatt ggtggtctcg gccttatcca     4380

ttgccaccaa aaccctcttt ttactaagaa acagtgagcc ttgttctggc agtccagaga     4440

atgacacggg aaaaaagcag atgaagagaa ggtggcagga gagggcacgt ggcccagcct     4500

cagtctctcc aactgagttc ctgcctgcct gcctttgctc agactgtttg ccccttactg     4560

ctcttctagg cctcattcta agccccttct ccaagttgcc tctccttatt tctccctgtc     4620

tgccaaaaaa tctttcccag ctcactaagt cagtctcacg cagtcactca ttaacccacc     4680

aatcactgat tgtgccggca catgaatgca ccaggtagat aagtagcatg gcgggttaat     4740

cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc     4800

gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc     4860

agtgagcgag cgagcgcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt     4920

cccaacagtt gcgcagcctg aatggcgaat ggcgattccg ttgcaatggc tggcggtaat     4980

attgttctgg atattaccag caaggccgat agtttgagtt cttctactca ggcaagtgat     5040

gttattacta atcaaagaag tattgcgaca acggttaatt tgcgtgatgg acagactctt     5100

ttactcggtg gcctcactga ttataaaaac acttctcagg attctggcgt accgttcctg     5160

tctaaaatcc ctttaatcgg cctcctgttt agctcccgct ctgattctaa cgaggaaagc     5220

acgttatacg tgctcgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc     5280

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc     5340

tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct     5400

aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa     5460

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc     5520

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact     5580

caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg     5640

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt     5700

tacaatttaa atatttgctt atacaatctt cctgtttttg gggcttttct gattatcaac     5760

cggggtacat atgattgaca tgctagtttt acgattaccg ttcatcgatt ctcttgtttg     5820

ctccagactc tcaggcaatg acctgatagc ctttgtagag acctctcaaa aatagctacc     5880

ctctccggca tgaatttatc agctagaacg gttgaatatc atattgatgg tgatttgact     5940

gtctccggcc tttctcaccc gtttgaatct ttacctacac attactcagg cattgcattt     6000

aaaatatatg agggttctaa aaatttttat ccttgcgttg aaataaaggc ttctcccgca     6060

aaagtattac agggtcataa tgtttttggt acaaccgatt tagctttatg ctctgaggct     6120

ttattgctta attttgctaa ttctttgcct tgcctgtatg atttattgga tgttggaatc     6180

gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca     6240

ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac     6300

ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga     6360

ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac     6420

gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt     6480

agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct     6540

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     6600

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg     6660

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg     6720

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc     6780

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat     6840

gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact     6900

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca     6960

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact     7020

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg     7080

atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg     7140

agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg     7200

aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg     7260

caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag     7320

ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc     7380

gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga     7440

tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat     7500

atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc     7560

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     7620

acccc                                                                 7625


<210>  23
<211>  9
<212>  PRT
<213>  Human immunodeficiency virus

<400>  23

Arg Lys Lys Arg Arg Gln Arg Arg Arg 
1               5                   


<210>  24
<211>  8
<212>  PRT
<213>  Human immunodeficiency virus

<400>  24

Lys Lys Arg Arg Gln Arg Arg Arg 
1               5               


<210>  25
<211>  8
<212>  PRT
<213>  Human immunodeficiency virus

<400>  25

Arg Lys Lys Arg Arg Gln Arg Arg 
1               5               


<210>  26
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered cell permeable peptide

<400>  26

Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   


<210>  27
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineer cell permeable peptide

<400>  27

Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   


<210>  28
<211>  16
<212>  PRT
<213>  Drosophila melanogaster

<400>  28

Arg Gln Ile Lys Ile Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys 
1               5                   10                  15      


<210>  29
<211>  16
<212>  PRT
<213>  Drosophila melanogaster

<400>  29

Arg Gln Ile Lys Ile Trp Phe Gln Asn Arg Arg Met Lys Ser Lys Lys 
1               5                   10                  15      


<210>  30
<211>  16
<212>  PRT
<213>  Drosophila melanogaster

<400>  30

Arg Gln Ile Lys Ile Trp Phe Gln Asn Lys Arg Ala Lys Ile Lys Lys 
1               5                   10                  15      


<210>  31
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  31

Arg Gln Ile Lys Ile Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys 
1               5                   10                  15      


<210>  32
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  32

Arg Val Ile Arg Val Trp Phe Gln Asn Lys Arg Cys Lys Asp Lys Lys 
1               5                   10                  15      


<210>  33
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  33

Gly Gly Gly 
1           


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  34

Asp Gly Gly Gly Ser 
1               5   


<210>  35
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  35

Thr Gly Glu Lys Pro 
1               5   


<210>  36
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  36

Gly Gly Arg Arg 
1               


<210>  37
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  37

Gly Gly Gly Gly Ser 
1               5   


<210>  38
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  38

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  39
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  39

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  40
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  40

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  41
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  41

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  42
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  42

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  43
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  43

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  44
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  44

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  45
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  45

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  46
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  46

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  47
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  47

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  48
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  48

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  49
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  49

Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10                  


<210>  50
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  50

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  51
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  51

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  52
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  52

Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10              


<210>  53
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  53

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  54
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  54

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  55
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  55

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  56
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  56

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  57
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  57

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  58
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  58

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  59
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  59

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  60
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  60

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  61
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  61

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  62
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  62

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  63
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  63

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  64
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  64

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  65
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  65

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  66
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  66

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  67
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  67

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  68
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  68

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  69
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus Kozak sequence

<400>  69
gccrccatgg                                                              10


