                         SEQUENCE LISTING

<110>  The State of Israel, Ministry of Agriculture & Rural 
       Development, Agricultural Research Organization (ARO) (Volcani 
       Center)
       IBDAH, Mwafaq
 
<120>  IDENTIFICATION AND CHARACTERIZATION OF UDP-GLUCOSE:PHLORETIN 
       4-O-GLUCOSYLTRANSFERASE FROM MALUS X DOMESTICA BORKH

<130>  69415

<150>  US 62/305,591
<151>  2016-03-09

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  1
atggtgcaac accgctttct ac                                                22


<210>  2
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  2
gtgcctagca tcttttaaaa ccttgatctg                                        30


<210>  3
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  3
cgataacgag gcgaagaaag a                                                 21


<210>  4
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  4
gagaacctcc acttgactac ac                                                22


<210>  5
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  5
tcgtccgtgg agaagagtta                                                   20


<210>  6
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  6
aatcatggat ggctggaaga g                                                 21


<210>  7
<211>  1446
<212>  DNA
<213>  Malus domestica

<400>  7
atggtgcaac accgctttct actcgtcaca tttccagctc aaggccacat caacccttcc       60

ctccaattcg ccaagcgcct tatcaacact acaggtgcgc atgtcaccta cgttactagt      120

ctgtcagccc atcgccgtat aggcaatggc tcaattccag atggattgac ctatgcgccc      180

ttctctgatg ggtacgacga tgggtttaag cccggcgaca acgtcgacga ctacatgtca      240

gagctgcggc gccgcggagt acaagccatt accgaccttg tagtcgcaag tgcaaacgag      300

ggtcaccctt acacttgcct agtctactca ttacttctcc cttggtcggc agggatggca      360

catgaacttc acctcccaag cgtgctgctt tggattcagc cagccacggt tttcgacatc      420

tactactatt actttaacgg gtacaaagat ctcatccggg ataatactag ttctggtacg      480

aacaatgtcc ttccatgttc aatagaatta ccaggtttgc cattatcttt cacaagccga      540

gaccttccct ccttcatggt ggatacaaat ccgtacaatt tcgccctccc gttgtttcaa      600

gaacagatgg agctgttgga aagagaaacc aatccgacca ttctagtcaa cacgttcgat      660

gcactagagc cggaagcctt aaaagcaatt gacaagtaca acttgattgg agttgggcca      720

ttgattccgt ccgctttctt ggacggcaag gatccatcgg acaagtcatt tggaggcgat      780

cttttccaaa aatcaaagga ctcttcatac ctcgagtggc tgaactcgaa gccagaaggg      840

tcggtgattt atgtgtcctt cggaagcatt tctgtgttgg gaaaggccca aatggaggaa      900

atcgcaaaag ggttgttgga ttgcggcctt ccgttcttgt gggttattag agataaggtc      960

ggcaagaagg gagacgataa cgaggcgaag aaagaagaag agatgttgag gtgcagagag     1020

gaattggaag agctcgggat gatagtgccg tggtgtagtc aagtggaggt tctctctagt     1080

ccttcgttgg gttgctttgt gacacattgt gggtggaatt caagtttgga gagcttggtt     1140

tcaggggtgc ccgtggtggc gtttcctcag tggacggacc aagggacgaa tgccaagttg     1200

atagaggact attggaagac aggagtgagg gtgacaccaa atgaggaggg gatagttacg     1260

ggtgaggagc tcaagaggtg cttggatttg gtattgggaa gtggggagat tggtgaagac     1320

gtgagaagga atgctaagaa atggaaagat ttggcaagag aggctgtgag tgaaggggac     1380

tcttcggaca agaatctcag ggctttcttg gatcagatca aggttttaaa agatgctagg     1440

cactag                                                                1446


<210>  8
<211>  481
<212>  PRT
<213>  Malus domestica

<400>  8

Met Val Gln His Arg Phe Leu Leu Val Thr Phe Pro Ala Gln Gly His 
1               5                   10                  15      


Ile Asn Pro Ser Leu Gln Phe Ala Lys Arg Leu Ile Asn Thr Thr Gly 
            20                  25                  30          


Ala His Val Thr Tyr Val Thr Ser Leu Ser Ala His Arg Arg Ile Gly 
        35                  40                  45              


Asn Gly Ser Ile Pro Asp Gly Leu Thr Tyr Ala Pro Phe Ser Asp Gly 
    50                  55                  60                  


Tyr Asp Asp Gly Phe Lys Pro Gly Asp Asn Val Asp Asp Tyr Met Ser 
65                  70                  75                  80  


Glu Leu Arg Arg Arg Gly Val Gln Ala Ile Thr Asp Leu Val Val Ala 
                85                  90                  95      


Ser Ala Asn Glu Gly His Pro Tyr Thr Cys Leu Val Tyr Ser Leu Leu 
            100                 105                 110         


Leu Pro Trp Ser Ala Gly Met Ala His Glu Leu His Leu Pro Ser Val 
        115                 120                 125             


Leu Leu Trp Ile Gln Pro Ala Thr Val Phe Asp Ile Tyr Tyr Tyr Tyr 
    130                 135                 140                 


Phe Asn Gly Tyr Lys Asp Leu Ile Arg Asp Asn Thr Ser Ser Gly Thr 
145                 150                 155                 160 


Asn Asn Val Leu Pro Cys Ser Ile Glu Leu Pro Gly Leu Pro Leu Ser 
                165                 170                 175     


Phe Thr Ser Arg Asp Leu Pro Ser Phe Met Val Asp Thr Asn Pro Tyr 
            180                 185                 190         


Asn Phe Ala Leu Pro Leu Phe Gln Glu Gln Met Glu Leu Leu Glu Arg 
        195                 200                 205             


Glu Thr Asn Pro Thr Ile Leu Val Asn Thr Phe Asp Ala Leu Glu Pro 
    210                 215                 220                 


Glu Ala Leu Lys Ala Ile Asp Lys Tyr Asn Leu Ile Gly Val Gly Pro 
225                 230                 235                 240 


Leu Ile Pro Ser Ala Phe Leu Asp Gly Lys Asp Pro Ser Asp Lys Ser 
                245                 250                 255     


Phe Gly Gly Asp Leu Phe Gln Lys Ser Lys Asp Ser Ser Tyr Leu Glu 
            260                 265                 270         


Trp Leu Asn Ser Lys Pro Glu Gly Ser Val Ile Tyr Val Ser Phe Gly 
        275                 280                 285             


Ser Ile Ser Val Leu Gly Lys Ala Gln Met Glu Glu Ile Ala Lys Gly 
    290                 295                 300                 


Leu Leu Asp Cys Gly Leu Pro Phe Leu Trp Val Ile Arg Asp Lys Val 
305                 310                 315                 320 


Gly Lys Lys Gly Asp Asp Asn Glu Ala Lys Lys Glu Glu Glu Met Leu 
                325                 330                 335     


Arg Cys Arg Glu Glu Leu Glu Glu Leu Gly Met Ile Val Pro Trp Cys 
            340                 345                 350         


Ser Gln Val Glu Val Leu Ser Ser Pro Ser Leu Gly Cys Phe Val Thr 
        355                 360                 365             


His Cys Gly Trp Asn Ser Ser Leu Glu Ser Leu Val Ser Gly Val Pro 
    370                 375                 380                 


Val Val Ala Phe Pro Gln Trp Thr Asp Gln Gly Thr Asn Ala Lys Leu 
385                 390                 395                 400 


Ile Glu Asp Tyr Trp Lys Thr Gly Val Arg Val Thr Pro Asn Glu Glu 
                405                 410                 415     


Gly Ile Val Thr Gly Glu Glu Leu Lys Arg Cys Leu Asp Leu Val Leu 
            420                 425                 430         


Gly Ser Gly Glu Ile Gly Glu Asp Val Arg Arg Asn Ala Lys Lys Trp 
        435                 440                 445             


Lys Asp Leu Ala Arg Glu Ala Val Ser Glu Gly Asp Ser Ser Asp Lys 
    450                 455                 460                 


Asn Leu Arg Ala Phe Leu Asp Gln Ile Lys Val Leu Lys Asp Ala Arg 
465                 470                 475                 480 


His 
    


<210>  9
<211>  396
<212>  PRT
<213>  Bacillus licheniformis

<400>  9

Met Gly His Lys His Ile Ala Ile Phe Asn Ile Pro Ala His Gly His 
1               5                   10                  15      


Ile Asn Pro Thr Leu Ala Leu Thr Ala Ser Leu Val Lys Arg Gly Tyr 
            20                  25                  30          


Arg Val Thr Tyr Pro Val Thr Asp Glu Phe Val Lys Ala Val Glu Glu 
        35                  40                  45              


Thr Gly Ala Glu Pro Leu Asn Tyr Arg Ser Thr Leu Asn Ile Asp Pro 
    50                  55                  60                  


Gln Gln Ile Arg Glu Leu Met Lys Asn Lys Lys Asp Met Ser Gln Ala 
65                  70                  75                  80  


Pro Leu Met Phe Ile Lys Glu Met Glu Glu Val Leu Pro Gln Leu Glu 
                85                  90                  95      


Ala Leu Tyr Glu Asn Asp Lys Pro Asp Leu Ile Leu Phe Asp Phe Met 
            100                 105                 110         


Ala Met Ala Gly Lys Leu Leu Ala Glu Lys Phe Gly Ile Glu Ala Val 
        115                 120                 125             


Arg Leu Cys Ser Thr Tyr Ala Gln Asn Glu His Phe Thr Phe Arg Ser 
    130                 135                 140                 


Ile Ser Glu Glu Phe Lys Ile Glu Leu Thr Pro Glu Gln Glu Asp Ala 
145                 150                 155                 160 


Leu Lys Asn Ser Asn Leu Pro Ser Phe Asn Phe Glu Asp Met Phe Glu 
                165                 170                 175     


Pro Ala Lys Leu Asn Ile Val Phe Met Pro Arg Ala Phe Gln Pro Tyr 
            180                 185                 190         


Gly Glu Thr Phe Asp Glu Arg Phe Ser Phe Val Gly Pro Ser Leu Ala 
        195                 200                 205             


Lys Arg Lys Phe Gln Glu Lys Glu Thr Pro Ile Ile Ser Asp Ser Gly 
    210                 215                 220                 


Arg Pro Val Met Leu Ile Ser Leu Gly Thr Ala Phe Asn Ala Trp Pro 
225                 230                 235                 240 


Glu Phe Tyr His Met Cys Ile Glu Ala Phe Arg Asp Thr Lys Trp Gln 
                245                 250                 255     


Val Ile Met Ala Val Gly Thr Thr Ile Asp Pro Glu Ser Phe Asp Asp 
            260                 265                 270         


Ile Pro Glu Asn Phe Ser Ile His Gln Arg Val Pro Gln Leu Glu Ile 
        275                 280                 285             


Leu Lys Lys Ala Glu Leu Phe Ile Thr His Gly Gly Met Asn Ser Thr 
    290                 295                 300                 


Met Glu Gly Leu Asn Ala Gly Val Pro Leu Val Ala Val Pro Gln Met 
305                 310                 315                 320 


Pro Glu Gln Glu Ile Thr Ala Arg Arg Val Glu Glu Leu Gly Leu Gly 
                325                 330                 335     


Lys His Leu Gln Pro Glu Asp Thr Thr Ala Ala Ser Leu Arg Glu Ala 
            340                 345                 350         


Val Ser Gln Thr Asp Gly Asp Pro His Val Leu Lys Arg Ile Gln Asp 
        355                 360                 365             


Met Gln Lys His Ile Lys Gln Ala Gly Gly Ala Glu Lys Ala Ala Asp 
    370                 375                 380                 


Glu Ile Glu Ala Phe Leu Ala Pro Ala Gly Val Lys 
385                 390                 395     


<210>  10
<211>  1763
<212>  DNA
<213>  Malus domestica

<400>  10
accttgaatg tatactccca caagaatttc aactgcactc agcttataag cccacagtac       60

caaatttttc aacaatcaac tacataccta cttattctac ttccaacaac tccacactcc      120

accgcgcacc tccggcaatc gcatacgccg ccatggtgca acaccgcttt ctactcgtca      180

catttccagc tcaaggccac atcaaccctt ccctccaatt cgccaagcgc cttatcaaca      240

ctacaggtgc gcatgtcacc tacgttacta gtctgtcagc ccatcgccgt ataggcaatg      300

gctcaattcc agatggattg acctatgcgc ccttctctga tgggtacgac gatgggttta      360

agcccggcga caacatcgac gactacatgt cagagctgcg gcgccgcgga gtacaagcca      420

ttaccgacct tgtagtcgca agtgcaaacg agggtcaccc ttacacttgc ctagtctact      480

cattacttct cccttggtcg gcagggatgg cacatgaact tcacctccca agcgtgctgc      540

tttggattca gccagccacg gttttcgaca tctactacta ttactttaac gggtacaaag      600

atctcatccg ggataatact agttctggta cgaacaatgt ccttccatgt tcaatagaat      660

taccaggttt gccattatct ttcacaagcc gagaccttcc ctccttcatg gtggatacaa      720

atccgtacaa tttcgccctc ccgttgtttc aagaacagat ggagctgttg gaaagagaaa      780

ccaatccgac cattctagtc aacacgttcg atgcactaga gccggaagcc ttaaaagcaa      840

ttgacaagta caacttgatt ggagttgggc cattgattcc gtccgctttc ttggacggca      900

aggatccatc ggacaagtca tttggaggcg atcttttcca aaaatcaaag gactcttcat      960

acctcgagtg gctgaactcg aagccagaag ggtcggtgat ttatgtgtcc ttcggaagca     1020

tttctgtgtt gggaaaggcc caaatggagg aaatcgcaaa agggttgttg gattgcggcc     1080

ttccgttctt gtgggttatt agagataagg tcgacaagaa gggagacgat aacgaggcga     1140

agaaagaaga agagatgttg aggtgcagag aggaattgga agagctcggg atgatagtgc     1200

cgtggtgtag tcaagtggag gttctctcta gtccttcgtt gggttgcttt gtgacacatt     1260

gtgggtggaa ttcaagtttg gagagcttgg tttcaggggt gcccgtggtg gcgtttcctc     1320

agtggacgga ccaagggacg aatgccaagt tgatagagga ctattggaag acaggagtga     1380

gggtgacacc aaatgaggag gggatagtta cgggtgagga gctcaagagg tgcttggatt     1440

tggtattggg aagtggggag attggtgaag acgtgagaag gaatgctaag aaatggaaag     1500

atttggcaag agaggctgtg agtgaagggg actcttcgga caagaatctc aaggctttct     1560

tggatcagat caaggtttta aaagatgcta ggcactagtc gggtggcggc ctaggacctg     1620

gggcctggca actacgcggc taggtgtcta ggcaggcgcc tagacggatt cgattaaatc     1680

tattatattt cgtgtaagta agtgtatgtt tatgcttaaa atatatatga tgtcatcata     1740

aacaacaaaa tagaatgaca tat                                             1763


<210>  11
<211>  1452
<212>  DNA
<213>  Malus domestica

<400>  11
atggcagcgc cgctgcccat cgaaatcgaa ccatcatcaa ctaatggtca accccatctc       60

gccgacgcct acaaccgtca cgtggctgtc gtagccttcc ctttcactag ccatgcaagc      120

gccttgcttg agaccgtgcg ccgcctagcc accgcccttc caaacactct cttctcgttc      180

ttcagcactt caaaatccaa cagctctctc ttttccaaca acagcattga taacatgccg      240

cgtaacataa gggtgtacga tgtggctgac ggggtgccgg aggggtacgt tttcgtgggc      300

aagccgcagg aggacataga gctcttcatg aatgccgcac cggaaaacat ccggaggagc      360

ttagacgctt ccgtggcgga catcgggaag cagatcagct gcttgatcac cgacgccttc      420

ctttggtttg gagtccactt ggctgacgag ttgggagtgc cttgggtcac tttctggatc      480

tccggactca aatccctctc cgttcatgtg catactgatc tcatccgcga cactattgga      540

actcaaggca ttacaggtcg tgaaaacgac ctcatcgtcg acaaaaatgt taacatccaa      600

ggtctctcca atgtacgaat caaagactta gcggaaggag tcattttcgg aaacttggac      660

tcggtaattt ccggcatgct acttcagatg ggacggctcc tcccccgtgc caccgcagtt      720

ttcatgaacg gcttcgaaga attggaactc cccataccaa acgacctaaa gtccaaagtc      780

aacaaactcc tcaacgtagg accttccaac gtagcatccc cgctgccacc gctgccgcca      840

tcagatgctt gcttgtcatg gctagacaag caacaggctc catcctccgt cgtgtacata      900

agcttcggga cagtggcgag cccagcggag aaggagcaga tggcaatagc ggaggccctg      960

gaagccaccg gagcaccctt cttgtggtct atcaaggaca gctgcaagac accgttgctg     1020

aacgagttct tgacaaaaac attgtcaaag ctgaacggga tggtggtgcc gtgggctcca     1080

cagccgcatg tactggccca cgattcggtc ggagccttcg tgtcgcattg cggctggaac     1140

tcgataatgg agactatagc aggacgggtg cccatgattt gtaggccata ttttgcagac     1200

cagaggctta atgcaaggat ggtggaggag gtgtttgaga tcggggtaac cgtggaggat     1260

ggagttttta ccagggaggg gctggtaaaa agcttggaag tggttttgtc gcctgaaagt     1320

gggaggaaat tcagagacaa tataaagagg gtcaaacaac tggcagtaga ggcggttgga     1380

ccacaaggga gctccactcg gaacttcaaa tcgctgttgg acatcgtatc aggatccaat     1440

tatcaagtat ag                                                         1452


<210>  12
<211>  483
<212>  PRT
<213>  Malus domestica

<400>  12

Met Ala Ala Pro Leu Pro Ile Glu Ile Glu Pro Ser Ser Thr Asn Gly 
1               5                   10                  15      


Gln Pro His Leu Ala Asp Ala Tyr Asn Arg His Val Ala Val Val Ala 
            20                  25                  30          


Phe Pro Phe Thr Ser His Ala Ser Ala Leu Leu Glu Thr Val Arg Arg 
        35                  40                  45              


Leu Ala Thr Ala Leu Pro Asn Thr Leu Phe Ser Phe Phe Ser Thr Ser 
    50                  55                  60                  


Lys Ser Asn Ser Ser Leu Phe Ser Asn Asn Ser Ile Asp Asn Met Pro 
65                  70                  75                  80  


Arg Asn Ile Arg Val Tyr Asp Val Ala Asp Gly Val Pro Glu Gly Tyr 
                85                  90                  95      


Val Phe Val Gly Lys Pro Gln Glu Asp Ile Glu Leu Phe Met Asn Ala 
            100                 105                 110         


Ala Pro Glu Asn Ile Arg Arg Ser Leu Asp Ala Ser Val Ala Asp Ile 
        115                 120                 125             


Gly Lys Gln Ile Ser Cys Leu Ile Thr Asp Ala Phe Leu Trp Phe Gly 
    130                 135                 140                 


Val His Leu Ala Asp Glu Leu Gly Val Pro Trp Val Thr Phe Trp Ile 
145                 150                 155                 160 


Ser Gly Leu Lys Ser Leu Ser Val His Val His Thr Asp Leu Ile Arg 
                165                 170                 175     


Asp Thr Ile Gly Thr Gln Gly Ile Thr Gly Arg Glu Asn Asp Leu Ile 
            180                 185                 190         


Val Asp Lys Asn Val Asn Ile Gln Gly Leu Ser Asn Val Arg Ile Lys 
        195                 200                 205             


Asp Leu Ala Glu Gly Val Ile Phe Gly Asn Leu Asp Ser Val Ile Ser 
    210                 215                 220                 


Gly Met Leu Leu Gln Met Gly Arg Leu Leu Pro Arg Ala Thr Ala Val 
225                 230                 235                 240 


Phe Met Asn Gly Phe Glu Glu Leu Glu Leu Pro Ile Pro Asn Asp Leu 
                245                 250                 255     


Lys Ser Lys Val Asn Lys Leu Leu Asn Val Gly Pro Ser Asn Val Ala 
            260                 265                 270         


Ser Pro Leu Pro Pro Leu Pro Pro Ser Asp Ala Cys Leu Ser Trp Leu 
        275                 280                 285             


Asp Lys Gln Gln Ala Pro Ser Ser Val Val Tyr Ile Ser Phe Gly Thr 
    290                 295                 300                 


Val Ala Ser Pro Ala Glu Lys Glu Gln Met Ala Ile Ala Glu Ala Leu 
305                 310                 315                 320 


Glu Ala Thr Gly Ala Pro Phe Leu Trp Ser Ile Lys Asp Ser Cys Lys 
                325                 330                 335     


Thr Pro Leu Leu Asn Glu Phe Leu Thr Lys Thr Leu Ser Lys Leu Asn 
            340                 345                 350         


Gly Met Val Val Pro Trp Ala Pro Gln Pro His Val Leu Ala His Asp 
        355                 360                 365             


Ser Val Gly Ala Phe Val Ser His Cys Gly Trp Asn Ser Ile Met Glu 
    370                 375                 380                 


Thr Ile Ala Gly Arg Val Pro Met Ile Cys Arg Pro Tyr Phe Ala Asp 
385                 390                 395                 400 


Gln Arg Leu Asn Ala Arg Met Val Glu Glu Val Phe Glu Ile Gly Val 
                405                 410                 415     


Thr Val Glu Asp Gly Val Phe Thr Arg Glu Gly Leu Val Lys Ser Leu 
            420                 425                 430         


Glu Val Val Leu Ser Pro Glu Ser Gly Arg Lys Phe Arg Asp Asn Ile 
        435                 440                 445             


Lys Arg Val Lys Gln Leu Ala Val Glu Ala Val Gly Pro Gln Gly Ser 
    450                 455                 460                 


Ser Thr Arg Asn Phe Lys Ser Leu Leu Asp Ile Val Ser Gly Ser Asn 
465                 470                 475                 480 


Tyr Gln Val 
            


