                              LISTADO DE SECUENCIAS

<110>  Universitat de Valncia
       Universidad de Castilla-La Mancha
       Universidad de Murcia
       Fundacin para la Investigacin del Hospital Universitario 
       y Politcnico La Fe
 
<120>  Mutante de Candida albicans y uso en terapia contra candidiasis

<130>  PCT-856

<150>  ES201630250
<151>  2016-03-03

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  31
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Primer PRI1-KpnI

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  Diana KpnI

<220>
<221>  source
<222>  (11)..(31)
<223>  Candida albicans

<400>  1
cacaggtacc ttagtattgt tactttgtta g                                      31


<210>  2
<211>  33
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Primer PRI1-XhoI

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  Diana XhoI

<220>
<221>  source
<222>  (11)..(33)
<223>  Candida albicans

<400>  2
cacactcgag tttgttatca tttctagtca atg                                    33


<210>  3
<211>  515
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Fragmento F1

<220>
<221>  source
<222>  (1)..(515)
<223>  Candida albicans, fragmento del gen PIR1

<220>
<221>  misc_feature
<222>  (291)..(293)
<223>  ATG iniciador

<400>  3
ttagtattgt tactttgtta gattttagca attaaaattt caataacaat actttattta       60

gaaacaagga ccacaaaatc aggattgaag aattaaagaa aaaaaaaact atttaaatag      120

aagtaattaa ccttctataa aataattcta ttttttttat tttttatatt ttttttgttt      180

ttaaaatcaa taatcttttc ttttctttct attatcatta gtttccaata cagatctaaa      240

acaactataa taacatcaac taaaacaacc ataacaataa caattaaatc atgaagtatt      300

ctacacttgt tagtattgct gcttttatta gcacttcttt agctgctact gttcctgatg      360

aacattattc aacattatca ccttcagcta aaattccatc tggtgctagt acagatttca      420

gtggtacttt tggtattcaa gttgttactg ttgaatcagc ttctgctctt tcaactgata      480

ctgctacttc tacattgact agaaatgata acaaa                                 515


<210>  4
<211>  36
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Primer PRI-NotI

<220>
<221>  misc_feature
<222>  (5)..(12)
<223>  Diana NotI

<220>
<221>  source
<222>  (13)..(36)
<223>  Candida albicans

<400>  4
cacagcggcc gctactgctg aaaatgttgc taaagc                                 36


<210>  5
<211>  33
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Primer PRI-SacI

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  Diana SacI

<220>
<221>  source
<222>  (11)..(33)
<223>  Candida albicans

<400>  5
cacagagctc tttaacagtt gacaaattca atg                                    33


<210>  6
<211>  413
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Fragmento F2

<220>
<221>  source
<222>  (1)..(413)
<223>  Candida albicans, fragmento del gen PIR1

<400>  6
tactgctgaa aatgttgcta aagctcaatc tgacggtcaa gcaattgcaa ctggttctcc       60

atcatccaac tcaactttat ctgatgacga tgacttatct tcaacaattc ctaaagcttg      120

ttcatctgca aacaatttag aaatgacatt acatgattct gttttaaaag atactcatga      180

acgttggggt gctattgtag ctaatcatca atttcaattc gatggaccaa ttcctcaagc      240

aggaacaatt tatagtgctg gttggtcaat taaagatgga tatttatatt tgggtgattc      300

taatatcttt tatcaatgtt tatcaggaga tttttataat ttatatgatg aaaatgttgc      360

taaacaatgt tcagctgtta aattaagtgt cattgaattt gtcaactgtt aaa             413


<210>  7
<211>  3517
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Plsmido pGEM-F1

<220>
<221>  source
<222>  (53)..(567)
<223>  Candida albicans: fragmento F1

<400>  7
gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga ttttagtatt       60

gttactttgt tagattttag caattaaaat ttcaataaca atactttatt tagaaacaag      120

gaccacaaaa tcaggattga agaattaaag aaaaaaaaaa ctatttaaat agaagtaatt      180

aaccttctat aaaataattc tatttttttt attttttata ttttttttgt ttttaaaatc      240

aataatcttt tcttttcttt ctattatcat tagtttccaa tacagatcta aaacaactat      300

aataacatca actaaaacaa ccataacaat aacaattaaa tcatgaagta ttctacactt      360

gttagtattg ctgcttttat tagcacttct ttagctgcta ctgttcctga tgaacattat      420

tcaacattat caccttcagc taaaattcca tctggtgcta gtacagattt cagtggtact      480

tttggtattc aagttgttac tgttgaatca gcttctgctc tttcaactga tactgctact      540

tctacattga ctagaaatga taacaaatat cactagtgcg gccgcctgca ggtcgaccat      600

atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat      660

agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt      720

ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc      780

taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc      840

cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct      900

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca      960

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     1020

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     1080

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     1140

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     1200

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     1260

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     1320

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     1380

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     1440

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     1500

aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc     1560

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     1620

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     1680

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     1740

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     1800

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     1860

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     1920

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     1980

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     2040

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     2100

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc     2160

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     2220

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     2280

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     2340

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     2400

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg     2460

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     2520

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     2580

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     2640

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     2700

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     2760

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     2820

gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag     2880

gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca     2940

ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag     3000

atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc     3060

aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc     3120

taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc     3180

ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa     3240

gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc     3300

acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca     3360

actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg     3420

gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta     3480

aaacgacggc cagtgaattg taatacgact cactata                              3517


<210>  8
<211>  3415
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Plsmido pGEM-F2

<220>
<221>  source
<222>  (53)..(434)
<223>  Candida albicans: fragmento F2

<400>  8
gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga tttactgctg       60

aaaatgttgc taaagctcaa tctgacggtc aagcaattgc aactggttct ccatcatcca      120

actcaacttt atctgatgac gatgacttat cttcaacaat tcctaaagct tgttcatctg      180

caaacaattt agaaatgaca ttacatgatt ctgttttaaa agatactcat gaacgttggg      240

gtgctattgt agctaatcat caatttcaat tcgatggacc aattcctcaa gcaggaacaa      300

tttatagtgc tggttggtca attaaagatg gatatttata tttgggtgat tctaatatct      360

tttatcaatg tttatcagga gatttttata atttatatga tgaaaatgtt gctaaacaat      420

gttcagctgt taaattaagt gtcattgaat ttgtcaactg ttaaatatca ctagtgcggc      480

cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt      540

ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat      600

tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg      660

ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag      720

tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt      780

ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg      840

ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg      900

gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag      960

gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga     1020

cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct     1080

ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc     1140

tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg     1200

gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc     1260

tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca     1320

ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag     1380

ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct     1440

ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc     1500

accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga     1560

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca     1620

cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat     1680

taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac     1740

caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt     1800

gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt     1860

gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag     1920

ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct     1980

attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt     2040

gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc     2100

tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt     2160

agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg     2220

gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg     2280

actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct     2340

tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc     2400

attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt     2460

tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt     2520

tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg     2580

aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat     2640

tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg     2700

cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag     2760

gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat     2820

ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa     2880

tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta     2940

ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca     3000

ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat     3060

cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg     3120

agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc     3180

acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt     3240

cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac     3300

gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt     3360

cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctata          3415


<210>  9
<211>  8015
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Plsmido pSFS2A-F1

<220>
<221>  source
<222>  (1)..(658)
<223>  Plsmido pSFS2A

<220>
<221>  source
<222>  (659)..(1173)
<223>  Candida albicans: fragmento F1

<220>
<221>  source
<222>  (1174)..(8015)
<223>  Plsmido pSFS2A

<400>  9
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt      660

agtattgtta ctttgttaga ttttagcaat taaaatttca ataacaatac tttatttaga      720

aacaaggacc acaaaatcag gattgaagaa ttaaagaaaa aaaaaactat ttaaatagaa      780

gtaattaacc ttctataaaa taattctatt ttttttattt tttatatttt ttttgttttt      840

aaaatcaata atcttttctt ttctttctat tatcattagt ttccaataca gatctaaaac      900

aactataata acatcaacta aaacaaccat aacaataaca attaaatcat gaagtattct      960

acacttgtta gtattgctgc ttttattagc acttctttag ctgctactgt tcctgatgaa     1020

cattattcaa cattatcacc ttcagctaaa attccatctg gtgctagtac agatttcagt     1080

ggtacttttg gtattcaagt tgttactgtt gaatcagctt ctgctctttc aactgatact     1140

gctacttcta cattgactag aaatgataac aaactcgagg aagttcctat actttctaga     1200

gaataggaac ttcggatcca ataatgattg gtttgatatt tttgtctagt accatctgta     1260

ccattacact taaattatct ttatatctgt ctaactcgac tgtctggatt tcattgatgt     1320

agtcgtatgc atcgttagtt ccaaaaaata ttgtcatcaa tttgatattg gtttccgact     1380

ctaaaatttt tggaagaatt tgtctagcgt gctctgagtt gtagccactg aaaccacggt     1440

taataacatc caattttcgg atatacacat tctgtaatgc tggatgaaag ccatactggg     1500

tacaactaaa ctgggtgatg gagtcaccga acaacacaaa tttaccgtat tccatgattg     1560

ctatggttga gaattttttt tttttcttgt cccacgccat ttttcaaatt atgcagttga     1620

gaatgttagt ttttgtgtac accccgttcg ctgaatattt cggaataatt caaagattgg     1680

ggagtggggg aggcgataga cgaagacacg gtataaaaat gggcaaaatt ttccccaact     1740

ttttgcagtg gtttaactaa taatcgtcga catgccacaa tttgatatat tatgtaaaac     1800

accacctaag gtgcttgttc gtcagtttgt ggaaaggttt gaaagacctt caggtgagaa     1860

aatagcatta tgtgctgctg aactaaccta tttatgttgg atgattacac ataacggaac     1920

agcaatcaag agagccacat tcatgagcta taatactatc ataagcaatt cgttgagttt     1980

cgatattgtc aataaatcac tccagtttaa atacaagacg caaaaagcaa caattttgga     2040

agcctcatta aagaaattga ttcctgcttg ggaatttaca attattcctt actatggaca     2100

aaaacatcaa tctgatatca ctgatattgt aagtagtttg caattacagt tcgaatcatc     2160

ggaagaagca gataagggaa atagccacag taaaaaaatg cttaaagcac ttctaagtga     2220

gggtgaaagc atctgggaga tcactgagaa aatactaaat tcgtttgagt atacttcgag     2280

atttacaaaa acaaaaactt tataccaatt cctcttccta gctactttca tcaattgtgg     2340

aagattcagc gatattaaga acgttgatcc gaaatcattt aaattagtcc aaaataagta     2400

tttgggagta ataatccagt gtttagtgac agagacaaag acaagcgtta gtaggcacat     2460

atacttcttt agcgcaaggg gtaggatcga tccacttgta tatttggatg aatttttgag     2520

gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca     2580

ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa     2640

tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt     2700

gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg     2760

gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat     2820

acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga     2880

aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct     2940

aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga     3000

ggtactagac tacctttcat cctacataaa tagacgcata taagagtgaa attctggaaa     3060

tctggaaatc tggttttgta ttcttgttat tcttcttttt gttattacat atataacttg     3120

ttactttttt aaaaaaatct ttgtatattt tataaatata taaaactaaa tttaagaaaa     3180

agagaaaaat gttttatttg agagattgaa attttacttg aatttagctt agcttttata     3240

aagtattatt atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga ctataaaata     3300

gccacccaag gcatttctat atcttgttgt tgttgttttc atcttctgta tcagaggaac     3360

ttattttatt attttcgtca cgggtatttt ctcttgtttg atgattcatc ccattcattc     3420

catcataaaa tgtcgagcgt caaaactaga gaataataaa gaaaacgatc ttttcaaaaa     3480

gaaaaaacct tttagttttc ctttgttgtt gttgtgggtg tgtgctattt atattatata     3540

gtttactcat aataccataa aatattcggt ttgattaggt tattttaata agctaatttg     3600

tttctaatcg tgtaatttat gctgtgtata ttaagtagtg tgtgcactgc ccaaaaatgt     3660

ttgttgttta tagtcggtta aagagaaaaa agaaaaaaag atccatacac acacgttaat     3720

tagttgttca acgtaataca ctcatatttt gttcttattt gctttcggtc gctgttctca     3780

ccaagattta ttgccaacga aacaattttt ttttatatat tttcagattt ttcttttttt     3840

cctttccttt ccttttctaa ttttcactcc tggttttctt tctttcttag aaacattatc     3900

tcgatattaa tattaaaaaa atataatcat tcaaaatgga cggtggtatg ttttagttta     3960

gcttcaattc taattgattg attaatcagt tgattggttt caatatgaca aatgggtagg     4020

gtgggaaaac ttcattttca attcagatca aacttttttg ttgtcgacat aatatttctc     4080

gtttgggatg ttactgtcac attaataata cacacacatc agcttataat tttgaaagta     4140

atttatcaga tatgttgtga cgatcaatgg aaatggctaa cttcaatgta tctgttcttc     4200

ccctttttca aagttcacgt tttttgattg attgattgat ctgtcggcag tggtttcaaa     4260

accattcggt gagtaatcct atcaatcaat gttacgacaa aaggctcaat attcaaaatt     4320

gcaatgtttt atgttttcct acgtgtactt gtgcaaggca attgattcaa cattgctttt     4380

ggtgtttgac gagtttctag tttggacttg tgttgttatc tgggctatac agatttcccg     4440

gctcactatg aatttttttt ttcgacgctc agtgcacaca actataaaca acacaaacac     4500

aaacacagca agaaaaaaaa aaaacgaaca ttgaattgaa accaagccaa ctgaaaaatt     4560

ccttatttaa atgactgtca tactaaccca tttttataga agaagttgct gctttagtta     4620

tcgataacgg ttctcatatg aaaatttcgg tgatccctga gcaggtggcg gaaacattgg     4680

atgctgagaa ccatttcatt gttcgtgaag tgttcgatgt gcacctatcc gaccaaggct     4740

ttgaactatc taccagaagt gtgagcccct accggaagga ttacatctcg gatgatgact     4800

ctgatgaaga ctctgcttgc tatggcgcat tcatcgacca agagcttgtc gggaagattg     4860

aactcaactc aacatggaac gatctagcct ctatcgaaca cattgttgtg tcgcacacgc     4920

accgaggcaa aggagtcgcg cacagtctca tcgaatttgc gaaaaagtgg gcactaagca     4980

gacagctcct tggcatacga ttagagacac aaacgaacaa tgtacctgcc tgcaatttgt     5040

acgcaaaatg tggctttact ctcggcggca ttgacctctt cacgtataaa actagacctc     5100

aagtctcgaa cgaaacagcg atgtactggt actggttctc gggagcacag gatgacgcct     5160

aacatatgtg aagtgtgaag ggggagattt tcactttatt agatttgtat atatgtataa     5220

taaataaata aataagttaa ataaataatt agataagggt ggtaattatt actatttaca     5280

atcaaaggtg gtcctgcagg aagttcctat actttctaga gaataggaac ttcagatcca     5340

ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg     5400

ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg     5460

ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa     5520

tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac     5580

ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     5640

gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     5700

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     5760

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     5820

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     5880

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     5940

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     6000

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     6060

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     6120

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     6180

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     6240

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag     6300

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     6360

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     6420

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     6480

attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttcga ccgaataaat     6540

acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg     6600

gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa     6660

ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttgt cgagattttc     6720

aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc     6780

ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa     6840

ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa     6900

gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattacg     6960

tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt     7020

tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg     7080

gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt     7140

ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac     7200

cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg     7260

caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc     7320

cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga     7380

gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg     7440

ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac     7500

ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc     7560

ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt     7620

gctcaggctc tccccgtgga ggtaataatt gacgatatga tccttttttt ctgatcaaaa     7680

gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     7740

agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     7800

accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     7860

gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat     7920

caagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat     7980

aggggttccg cgcacatttc cccgaaaagt gccac                                8015


<210>  10
<211>  8419
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Plsmido pPDC

<220>
<221>  source
<222>  (1)..(658)
<223>  Plsmido PSFS2A

<220>
<221>  source
<222>  (659)..(1174)
<223>  Candida albicans: fragmento F1

<220>
<221>  source
<222>  (1175)..(5365)
<223>  Plsmido PSFS2A

<220>
<221>  source
<222>  (5366)..(7267)
<223>  Candida albicans: fragmento F2

<220>
<221>  source
<222>  (7268)..(7509)
<223>  Plsmido PSFS2A

<400>  10
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggttgcggg cctcttcgct attacgccag ctggcgaaag      540

ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt      600

gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtacct      660

tagtattgtt actttgttag attttagcaa ttaaaatttc aataacaata ctttatttag      720

aaacaaggac cacaaaatca ggattgaaga attaaagaaa aaaaaaacta tttaaataga      780

agtaattaac cttctataaa ataattctat tttttttatt ttttatattt tttttgtttt      840

taaaatcaat aatcttttct tttctttcta ttatcattag tttccaatac agatctaaaa      900

caactataat aacatcaact aaaacaacca taacaataac aattaaatca tgaagtattc      960

tacacttgtt agtattgctg cttttattag cacttcttta gctgctactg ttcctgatga     1020

acattattca acattatcac cttcagctaa aattccatct ggtgctagta cagatttcag     1080

tggtactttt ggtattcaag ttgttactgt tgaatcagct tctgctcttt caactgatac     1140

tgctacttct acattgacta gaaatgataa caaactcgag gaagttccta tactttctag     1200

agaataggaa cttcggatcc aataatgatt ggtttgatat ttttgtctag taccatctgt     1260

accattacac ttaaattatc tttatatctg tctaactcga ctgtctggat ttcattgatg     1320

tagtcgtatg catcgttagt tccaaaaaat attgtcatca atttgatatt ggtttccgac     1380

tctaaaattt ttggaagaat ttgtctagcg tgctctgagt tgtagccact gaaaccacgg     1440

ttaataacat ccaattttcg gatatacaca ttctgtaatg ctggatgaaa gccatactgg     1500

gtacaactaa actgggtgat ggagtcaccg aacaacacaa atttaccgta ttccatgatt     1560

gctatggttg agaatttttt ttttttcttg tcccacgcca tttttcaaat tatgcagttg     1620

agaatgttag tttttgtgta caccccgttc gctgaatatt tcggaataat tcaaagattg     1680

gggagtgggg gaggcgatag acgaagacac ggtataaaaa tgggcaaaat tttccccaac     1740

tttttgcagt ggtttaacta ataatcgtcg acatgccaca atttgatata ttatgtaaaa     1800

caccacctaa ggtgcttgtt cgtcagtttg tggaaaggtt tgaaagacct tcaggtgaga     1860

aaatagcatt atgtgctgct gaactaacct atttatgttg gatgattaca cataacggaa     1920

cagcaatcaa gagagccaca ttcatgagct ataatactat cataagcaat tcgttgagtt     1980

tcgatattgt caataaatca ctccagttta aatacaagac gcaaaaagca acaattttgg     2040

aagcctcatt aaagaaattg attcctgctt gggaatttac aattattcct tactatggac     2100

aaaaacatca atctgatatc actgatattg taagtagttt gcaattacag ttcgaatcat     2160

cggaagaagc agataaggga aatagccaca gtaaaaaaat gcttaaagca cttctaagtg     2220

agggtgaaag catctgggag atcactgaga aaatactaaa ttcgtttgag tatacttcga     2280

gatttacaaa aacaaaaact ttataccaat tcctcttcct agctactttc atcaattgtg     2340

gaagattcag cgatattaag aacgttgatc cgaaatcatt taaattagtc caaaataagt     2400

atttgggagt aataatccag tgtttagtga cagagacaaa gacaagcgtt agtaggcaca     2460

tatacttctt tagcgcaagg ggtaggatcg atccacttgt atatttggat gaatttttga     2520

ggaattctga accagtccta aaacgagtaa ataggaccgg caattcttca agcaataaac     2580

aggaatacca attattaaaa gataacttag tcagatcgta caataaagct ttgaagaaaa     2640

atgcgcctta ttcaatcttt gctataaaaa atggcccaaa atctcacatt ggaagacatt     2700

tgatgacctc atttctttca atgaagggcc taacggagtt gactaatgtt gtgggaaatt     2760

ggagcgataa gcgtgcttct gccgtggcca ggacaacgta tactcatcag ataacagcaa     2820

tacctgatca ctacttcgca ctagtttctc ggtactatgc atatgatcca atatcaaagg     2880

aaatgatagc attgaaggat gagactaatc caattgagga gtggcagcat atagaacagc     2940

taaagggtag tgctgaagga agcatacgat accccgcatg gaatgggata atatcacagg     3000

aggtactaga ctacctttca tcctacataa atagacgcat ataagagtga aattctggaa     3060

atctggaaat ctggttttgt attcttgtta ttcttctttt tgttattaca tatataactt     3120

gttacttttt taaaaaaatc tttgtatatt ttataaatat ataaaactaa atttaagaaa     3180

aagagaaaaa tgttttattt gagagattga aattttactt gaatttagct tagcttttat     3240

aaagtattat tatgtaaaaa aacaaaacaa atatacatta aaaagttaag actataaaat     3300

agccacccaa ggcatttcta tatcttgttg ttgttgtttt catcttctgt atcagaggaa     3360

cttattttat tattttcgtc acgggtattt tctcttgttt gatgattcat cccattcatt     3420

ccatcataaa atgtcgagcg tcaaaactag agaataataa agaaaacgat cttttcaaaa     3480

agaaaaaacc ttttagtttt cctttgttgt tgttgtgggt gtgtgctatt tatattatat     3540

agtttactca taataccata aaatattcgg tttgattagg ttattttaat aagctaattt     3600

gtttctaatc gtgtaattta tgctgtgtat attaagtagt gtgtgcactg cccaaaaatg     3660

tttgttgttt atagtcggtt aaagagaaaa aagaaaaaaa gatccataca cacacgttaa     3720

ttagttgttc aacgtaatac actcatattt tgttcttatt tgctttcggt cgctgttctc     3780

accaagattt attgccaacg aaacaatttt tttttatata ttttcagatt tttctttttt     3840

tcctttcctt tccttttcta attttcactc ctggttttct ttctttctta gaaacattat     3900

ctcgatatta atattaaaaa aatataatca ttcaaaatgg acggtggtat gttttagttt     3960

agcttcaatt ctaattgatt gattaatcag ttgattggtt tcaatatgac aaatgggtag     4020

ggtgggaaaa cttcattttc aattcagatc aaactttttt gttgtcgaca taatatttct     4080

cgtttgggat gttactgtca cattaataat acacacacat cagcttataa ttttgaaagt     4140

aatttatcag atatgttgtg acgatcaatg gaaatggcta acttcaatgt atctgttctt     4200

cccctttttc aaagttcacg ttttttgatt gattgattga tctgtcggca gtggtttcaa     4260

aaccattcgg tgagtaatcc tatcaatcaa tgttacgaca aaaggctcaa tattcaaaat     4320

tgcaatgttt tatgttttcc tacgtgtact tgtgcaaggc aattgattca acattgcttt     4380

tggtgtttga cgagtttcta gtttggactt gtgttgttat ctgggctata cagatttccc     4440

ggctcactat gaattttttt tttcgacgct cagtgcacac aactataaac aacacaaaca     4500

caaacacagc aagaaaaaaa aaaaacgaac attgaattga aaccaagcca actgaaaaat     4560

tccttattta aatgactgtc atactaaccc atttttatag aagaagttgc tgctttagtt     4620

atcgataacg gttctcatat gaaaatttcg gtgatccctg agcaggtggc ggaaacattg     4680

gatgctgaga accatttcat tgttcgtgaa gtgttcgatg tgcacctatc cgaccaaggc     4740

tttgaactat ctaccagaag tgtgagcccc taccggaagg attacatctc ggatgatgac     4800

tctgatgaag actctgcttg ctatggcgca ttcatcgacc aagagcttgt cgggaagatt     4860

gaactcaact caacatggaa cgatctagcc tctatcgaac acattgttgt gtcgcacacg     4920

caccgaggca aaggagtcgc gcacagtctc atcgaatttg cgaaaaagtg ggcactaagc     4980

agacagctcc ttggcatacg attagagaca caaacgaaca atgtacctgc ctgcaatttg     5040

tacgcaaaat gtggctttac tctcggcggc attgacctct tcacgtataa aactagacct     5100

caagtctcga acgaaacagc gatgtactgg tactggttct cgggagcaca ggatgacgcc     5160

taacatatgt gaagtgtgaa gggggagatt ttcactttat tagatttgta tatatgtata     5220

ataaataaat aaataagtta aataaataat tagataaggg tggtaattat tactatttac     5280

aatcaaaggt ggtcctgcag gaagttccta tactttctag agaataggaa cttcagatcc     5340

actagttcta gagcggccgc tactgctgaa aatgttgcta aagctcaatc tgacggtcaa     5400

gcaattgcaa ctggttctcc atcatccaac tcaactttat ctgatgacga tgacttatct     5460

tcaacaattc ctaaagcttg ttcatctgca aacaatttag aaatgacatt acatgattct     5520

gttttaaaag atactcatga acgttggggt gctattgtag ctaatcatca atttcaattc     5580

gatggaccaa ttcctcaagc aggaacaatt tatagtgctg gttggtcaat taaagatgga     5640

tatttatatt tgggtgattc taatatcttt tatcaatgtt tatcaggaga tttttataat     5700

ttatatgatg aaaatgttgc taaacaatgt tcagctgtta aattaagtgt cattgaattt     5760

gtcaactgtt aaagagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg     5820

cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca     5880

acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca     5940

cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc     6000

attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt     6060

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     6120

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     6180

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     6240

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     6300

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     6360

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     6420

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     6480

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     6540

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     6600

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     6660

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     6720

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     6780

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     6840

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat     6900

tatcaaaaag gatcttcacc tagatccttt tcgaccgaat aaatacctgt gacggaagat     6960

cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac     7020

ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa     7080

ataagatcac taccgggcgt attttttgag ttgtcgagat tttcaggagc taaggaagct     7140

aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa     7200

gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg     7260

gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt     7320

attcacattc ttgcccgcct gatgaatgct catccggaat tacgtatggc aatgaaagac     7380

ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact     7440

gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata     7500

tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt     7560

gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac     7620

gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa     7680

ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc     7740

catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg     7800

taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt     7860

gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc gtcggttcag     7920

ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta ttgactaccg     7980

gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag gctctccccg     8040

tggaggtaat aattgacgat atgatccttt ttttctgatc aaaagtgctc atcattggaa     8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt     8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt     8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt     8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcaaggg ttattgtctc     8340

atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca     8400

tttccccgaa aagtgccac                                                  8419


<210>  11
<211>  5126
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Fragmento F3: casete integrativo

<220>
<221>  Misc_feature
<222>  (1)..(6)
<223>  Diana KpnI

<220>
<221>  Misc_feature
<222>  (1)..(5126)
<223>  Fragmento F3: casete de disrupcin flanqueado por las dianas KpnI
       y SacI

<220>
<221>  Misc_feature
<222>  (7)..(522)
<223>  Fragmento F1 de Candida albicans

<220>
<221>  Misc_feature
<222>  (527)..(561)
<223>  Secuencia FRT

<220>
<221>  Promoter
<222>  (566)..(1113)
<223>  Promotor Mal2p del gen MAL2 de Candida albicans

<220>
<221>  gene
<222>  (1120)..(2391)
<223>  Gen FLP de Candida albicans, CaFLP

<220>
<221>  CDS
<222>  (1120)..(2391)
<223>  Gen FLP de Candida albicans, CaFLP

<220>
<221>  promoter
<222>  (2785)..(3283)
<223>  Promotor del gen ACT1 de Candida albicans

<220>
<221>  gene
<222>  (3284)..(4507)
<223>  Gen SAT1

<220>
<221>  exon
<222>  (3284)..(3293)
<223>  CDS gen SAT1, parte1

<220>
<221>  exon
<222>  (3948)..(4507)
<223>  CDS gen SAT1, parte2

<220>
<221>  Misc_feature
<222>  (4648)..(4681)
<223>  Secuencia FRT

<220>
<221>  Misc_feature
<222>  (4707)..(5120)
<223>  Fragmento F2 de Candida albicans

<220>
<221>  Misc_feature
<222>  (5121)..(5126)
<223>  Diana SacI

<400>  11
ggtaccttag tattgttact ttgttagatt ttagcaatta aaatttcaat aacaatactt       60

tatttagaaa caaggaccac aaaatcagga ttgaagaatt aaagaaaaaa aaaactattt      120

aaatagaagt aattaacctt ctataaaata attctatttt ttttattttt tatatttttt      180

ttgtttttaa aatcaataat cttttctttt ctttctatta tcattagttt ccaatacaga      240

tctaaaacaa ctataataac atcaactaaa acaaccataa caataacaat taaatcatga      300

agtattctac acttgttagt attgctgctt ttattagcac ttctttagct gctactgttc      360

ctgatgaaca ttattcaaca ttatcacctt cagctaaaat tccatctggt gctagtacag      420

atttcagtgg tacttttggt attcaagttg ttactgttga atcagcttct gctctttcaa      480

ctgatactgc tacttctaca ttgactagaa atgataacaa actcgaggaa gttcctatac      540

tttctagaga ataggaactt cggatccaat aatgattggt ttgatatttt tgtctagtac      600

catctgtacc attacactta aattatcttt atatctgtct aactcgactg tctggatttc      660

attgatgtag tcgtatgcat cgttagttcc aaaaaatatt gtcatcaatt tgatattggt      720

ttccgactct aaaatttttg gaagaatttg tctagcgtgc tctgagttgt agccactgaa      780

accacggtta ataacatcca attttcggat atacacattc tgtaatgctg gatgaaagcc      840

atactgggta caactaaact gggtgatgga gtcaccgaac aacacaaatt taccgtattc      900

catgattgct atggttgaga attttttttt tttcttgtcc cacgccattt ttcaaattat      960

gcagttgaga atgttagttt ttgtgtacac cccgttcgct gaatatttcg gaataattca     1020

aagattgggg agtgggggag gcgatagacg aagacacggt ataaaaatgg gcaaaatttt     1080

ccccaacttt ttgcagtggt ttaactaata atcgtcgac atg cca caa ttt gat        1134
                                           Met Pro Gln Phe Asp            
                                           1               5              

ata tta tgt aaa aca cca cct aag gtg ctt gtt cgt cag ttt gtg gaa       1182
Ile Leu Cys Lys Thr Pro Pro Lys Val Leu Val Arg Gln Phe Val Glu           
                10                  15                  20                

agg ttt gaa aga cct tca ggt gag aaa ata gca tta tgt gct gct gaa       1230
Arg Phe Glu Arg Pro Ser Gly Glu Lys Ile Ala Leu Cys Ala Ala Glu           
            25                  30                  35                    

cta acc tat tta tgt tgg atg att aca cat aac gga aca gca atc aag       1278
Leu Thr Tyr Leu Cys Trp Met Ile Thr His Asn Gly Thr Ala Ile Lys           
        40                  45                  50                        

aga gcc aca ttc atg agc tat aat act atc ata agc aat tcg ttg agt       1326
Arg Ala Thr Phe Met Ser Tyr Asn Thr Ile Ile Ser Asn Ser Leu Ser           
    55                  60                  65                            

ttc gat att gtc aat aaa tca ctc cag ttt aaa tac aag acg caa aaa       1374
Phe Asp Ile Val Asn Lys Ser Leu Gln Phe Lys Tyr Lys Thr Gln Lys           
70                  75                  80                  85            

gca aca att ttg gaa gcc tca tta aag aaa ttg att cct gct tgg gaa       1422
Ala Thr Ile Leu Glu Ala Ser Leu Lys Lys Leu Ile Pro Ala Trp Glu           
                90                  95                  100               

ttt aca att att cct tac tat gga caa aaa cat caa tct gat atc act       1470
Phe Thr Ile Ile Pro Tyr Tyr Gly Gln Lys His Gln Ser Asp Ile Thr           
            105                 110                 115                   

gat att gta agt agt ttg caa tta cag ttc gaa tca tcg gaa gaa gca       1518
Asp Ile Val Ser Ser Leu Gln Leu Gln Phe Glu Ser Ser Glu Glu Ala           
        120                 125                 130                       

gat aag gga aat agc cac agt aaa aaa atg ctt aaa gca ctt cta agt       1566
Asp Lys Gly Asn Ser His Ser Lys Lys Met Leu Lys Ala Leu Leu Ser           
    135                 140                 145                           

gag ggt gaa agc atc tgg gag atc act gag aaa ata cta aat tcg ttt       1614
Glu Gly Glu Ser Ile Trp Glu Ile Thr Glu Lys Ile Leu Asn Ser Phe           
150                 155                 160                 165           

gag tat act tcg aga ttt aca aaa aca aaa act tta tac caa ttc ctc       1662
Glu Tyr Thr Ser Arg Phe Thr Lys Thr Lys Thr Leu Tyr Gln Phe Leu           
                170                 175                 180               

ttc cta gct act ttc atc aat tgt gga aga ttc agc gat att aag aac       1710
Phe Leu Ala Thr Phe Ile Asn Cys Gly Arg Phe Ser Asp Ile Lys Asn           
            185                 190                 195                   

gtt gat ccg aaa tca ttt aaa tta gtc caa aat aag tat ttg gga gta       1758
Val Asp Pro Lys Ser Phe Lys Leu Val Gln Asn Lys Tyr Leu Gly Val           
        200                 205                 210                       

ata atc cag tgt tta gtg aca gag aca aag aca agc gtt agt agg cac       1806
Ile Ile Gln Cys Leu Val Thr Glu Thr Lys Thr Ser Val Ser Arg His           
    215                 220                 225                           

ata tac ttc ttt agc gca agg ggt agg atc gat cca ctt gta tat ttg       1854
Ile Tyr Phe Phe Ser Ala Arg Gly Arg Ile Asp Pro Leu Val Tyr Leu           
230                 235                 240                 245           

gat gaa ttt ttg agg aat tct gaa cca gtc cta aaa cga gta aat agg       1902
Asp Glu Phe Leu Arg Asn Ser Glu Pro Val Leu Lys Arg Val Asn Arg           
                250                 255                 260               

acc ggc aat tct tca agc aat aaa cag gaa tac caa tta tta aaa gat       1950
Thr Gly Asn Ser Ser Ser Asn Lys Gln Glu Tyr Gln Leu Leu Lys Asp           
            265                 270                 275                   

aac tta gtc aga tcg tac aat aaa gct ttg aag aaa aat gcg cct tat       1998
Asn Leu Val Arg Ser Tyr Asn Lys Ala Leu Lys Lys Asn Ala Pro Tyr           
        280                 285                 290                       

tca atc ttt gct ata aaa aat ggc cca aaa tct cac att gga aga cat       2046
Ser Ile Phe Ala Ile Lys Asn Gly Pro Lys Ser His Ile Gly Arg His           
    295                 300                 305                           

ttg atg acc tca ttt ctt tca atg aag ggc cta acg gag ttg act aat       2094
Leu Met Thr Ser Phe Leu Ser Met Lys Gly Leu Thr Glu Leu Thr Asn           
310                 315                 320                 325           

gtt gtg gga aat tgg agc gat aag cgt gct tct gcc gtg gcc agg aca       2142
Val Val Gly Asn Trp Ser Asp Lys Arg Ala Ser Ala Val Ala Arg Thr           
                330                 335                 340               

acg tat act cat cag ata aca gca ata cct gat cac tac ttc gca cta       2190
Thr Tyr Thr His Gln Ile Thr Ala Ile Pro Asp His Tyr Phe Ala Leu           
            345                 350                 355                   

gtt tct cgg tac tat gca tat gat cca ata tca aag gaa atg ata gca       2238
Val Ser Arg Tyr Tyr Ala Tyr Asp Pro Ile Ser Lys Glu Met Ile Ala           
        360                 365                 370                       

ttg aag gat gag act aat cca att gag gag tgg cag cat ata gaa cag       2286
Leu Lys Asp Glu Thr Asn Pro Ile Glu Glu Trp Gln His Ile Glu Gln           
    375                 380                 385                           

cta aag ggt agt gct gaa gga agc ata cga tac ccc gca tgg aat ggg       2334
Leu Lys Gly Ser Ala Glu Gly Ser Ile Arg Tyr Pro Ala Trp Asn Gly           
390                 395                 400                 405           

ata ata tca cag gag gta cta gac tac ctt tca tcc tac ata aat aga       2382
Ile Ile Ser Gln Glu Val Leu Asp Tyr Leu Ser Ser Tyr Ile Asn Arg           
                410                 415                 420               

cgc ata taa gagtgaaatt ctggaaatct ggaaatctgg ttttgtattc               2431
Arg Ile                                                                   
                                                                          

ttgttattct tctttttgtt attacatata taacttgtta cttttttaaa aaaatctttg     2491

tatattttat aaatatataa aactaaattt aagaaaaaga gaaaaatgtt ttatttgaga     2551

gattgaaatt ttacttgaat ttagcttagc ttttataaag tattattatg taaaaaaaca     2611

aaacaaatat acattaaaaa gttaagacta taaaatagcc acccaaggca tttctatatc     2671

ttgttgttgt tgttttcatc ttctgtatca gaggaactta ttttattatt ttcgtcacgg     2731

gtattttctc ttgtttgatg attcatccca ttcattccat cataaaatgt cgagcgtcaa     2791

aactagagaa taataaagaa aacgatcttt tcaaaaagaa aaaacctttt agttttcctt     2851

tgttgttgtt gtgggtgtgt gctatttata ttatatagtt tactcataat accataaaat     2911

attcggtttg attaggttat tttaataagc taatttgttt ctaatcgtgt aatttatgct     2971

gtgtatatta agtagtgtgt gcactgccca aaaatgtttg ttgtttatag tcggttaaag     3031

agaaaaaaga aaaaaagatc catacacaca cgttaattag ttgttcaacg taatacactc     3091

atattttgtt cttatttgct ttcggtcgct gttctcacca agatttattg ccaacgaaac     3151

aatttttttt tatatatttt cagatttttc tttttttcct ttcctttcct tttctaattt     3211

tcactcctgg ttttctttct ttcttagaaa cattatctcg atattaatat taaaaaaata     3271

taatcattca aa atg gac ggt g gtatgtttta gtttagcttc aattctaatt          3323
              Met Asp Gly                                                 
                  425                                                     

gattgattaa tcagttgatt ggtttcaata tgacaaatgg gtagggtggg aaaacttcat     3383

tttcaattca gatcaaactt ttttgttgtc gacataatat ttctcgtttg ggatgttact     3443

gtcacattaa taatacacac acatcagctt ataattttga aagtaattta tcagatatgt     3503

tgtgacgatc aatggaaatg gctaacttca atgtatctgt tcttcccctt tttcaaagtt     3563

cacgtttttt gattgattga ttgatctgtc ggcagtggtt tcaaaaccat tcggtgagta     3623

atcctatcaa tcaatgttac gacaaaaggc tcaatattca aaattgcaat gttttatgtt     3683

ttcctacgtg tacttgtgca aggcaattga ttcaacattg cttttggtgt ttgacgagtt     3743

tctagtttgg acttgtgttg ttatctgggc tatacagatt tcccggctca ctatgaattt     3803

tttttttcga cgctcagtgc acacaactat aaacaacaca aacacaaaca cagcaagaaa     3863

aaaaaaaaac gaacattgaa ttgaaaccaa gccaactgaa aaattcctta tttaaatgac     3923

tgtcatacta acccattttt atag aa  gaa gtt gct gct tta gtt atc gat        3973
                           Glu Glu Val Ala Ala Leu Val Ile Asp            
                                       430                 435            

aac ggt tct cat atg aaa att tcg gtg atc cct gag cag gtg gcg gaa       4021
Asn Gly Ser His Met Lys Ile Ser Val Ile Pro Glu Gln Val Ala Glu           
                440                 445                 450               

aca ttg gat gct gag aac cat ttc att gtt cgt gaa gtg ttc gat gtg       4069
Thr Leu Asp Ala Glu Asn His Phe Ile Val Arg Glu Val Phe Asp Val           
            455                 460                 465                   

cac cta tcc gac caa ggc ttt gaa cta tct acc aga agt gtg agc ccc       4117
His Leu Ser Asp Gln Gly Phe Glu Leu Ser Thr Arg Ser Val Ser Pro           
        470                 475                 480                       

tac cgg aag gat tac atc tcg gat gat gac tct gat gaa gac tct gct       4165
Tyr Arg Lys Asp Tyr Ile Ser Asp Asp Asp Ser Asp Glu Asp Ser Ala           
    485                 490                 495                           

tgc tat ggc gca ttc atc gac caa gag ctt gtc ggg aag att gaa ctc       4213
Cys Tyr Gly Ala Phe Ile Asp Gln Glu Leu Val Gly Lys Ile Glu Leu           
500                 505                 510                 515           

aac tca aca tgg aac gat cta gcc tct atc gaa cac att gtt gtg tcg       4261
Asn Ser Thr Trp Asn Asp Leu Ala Ser Ile Glu His Ile Val Val Ser           
                520                 525                 530               

cac acg cac cga ggc aaa gga gtc gcg cac agt ctc atc gaa ttt gcg       4309
His Thr His Arg Gly Lys Gly Val Ala His Ser Leu Ile Glu Phe Ala           
            535                 540                 545                   

aaa aag tgg gca cta agc aga cag ctc ctt ggc ata cga tta gag aca       4357
Lys Lys Trp Ala Leu Ser Arg Gln Leu Leu Gly Ile Arg Leu Glu Thr           
        550                 555                 560                       

caa acg aac aat gta cct gcc tgc aat ttg tac gca aaa tgt ggc ttt       4405
Gln Thr Asn Asn Val Pro Ala Cys Asn Leu Tyr Ala Lys Cys Gly Phe           
    565                 570                 575                           

act ctc ggc ggc att gac ctc ttc acg tat aaa act aga cct caa gtc       4453
Thr Leu Gly Gly Ile Asp Leu Phe Thr Tyr Lys Thr Arg Pro Gln Val           
580                 585                 590                 595           

tcg aac gaa aca gcg atg tac tgg tac tgg ttc tcg gga gca cag gat       4501
Ser Asn Glu Thr Ala Met Tyr Trp Tyr Trp Phe Ser Gly Ala Gln Asp           
                600                 605                 610               

gac gcc taacatatgt gaagtgtgaa gggggagatt ttcactttat tagatttgta        4557
Asp Ala                                                                   
                                                                          

tatatgtata ataaataaat aaataagtta aataaataat tagataaggg tggtaattat     4617

tactatttac aatcaaaggt ggtcctgcag gaagttccta tactttctag agaataggaa     4677

cttcagatcc actagttcta gagcggccgc tactgctgaa aatgttgcta aagctcaatc     4737

tgacggtcaa gcaattgcaa ctggttctcc atcatccaac tcaactttat ctgatgacga     4797

tgacttatct tcaacaattc ctaaagcttg ttcatctgca aacaatttag aaatgacatt     4857

acatgattct gttttaaaag atactcatga acgttggggt gctattgtag ctaatcatca     4917

atttcaattc gatggaccaa ttcctcaagc aggaacaatt tatagtgctg gttggtcaat     4977

taaagatgga tatttatatt tgggtgattc taatatcttt tatcaatgtt tatcaggaga     5037

tttttataat ttatatgatg aaaatgttgc taaacaatgt tcagctgtta aattaagtgt     5097

cattgaattt gtcaactgtt aaagagctc                                       5126


<210>  12
<211>  423
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Constructo sinttico

<400>  12

Met Pro Gln Phe Asp Ile Leu Cys Lys Thr Pro Pro Lys Val Leu Val 
1               5                   10                  15      


Arg Gln Phe Val Glu Arg Phe Glu Arg Pro Ser Gly Glu Lys Ile Ala 
            20                  25                  30          


Leu Cys Ala Ala Glu Leu Thr Tyr Leu Cys Trp Met Ile Thr His Asn 
        35                  40                  45              


Gly Thr Ala Ile Lys Arg Ala Thr Phe Met Ser Tyr Asn Thr Ile Ile 
    50                  55                  60                  


Ser Asn Ser Leu Ser Phe Asp Ile Val Asn Lys Ser Leu Gln Phe Lys 
65                  70                  75                  80  


Tyr Lys Thr Gln Lys Ala Thr Ile Leu Glu Ala Ser Leu Lys Lys Leu 
                85                  90                  95      


Ile Pro Ala Trp Glu Phe Thr Ile Ile Pro Tyr Tyr Gly Gln Lys His 
            100                 105                 110         


Gln Ser Asp Ile Thr Asp Ile Val Ser Ser Leu Gln Leu Gln Phe Glu 
        115                 120                 125             


Ser Ser Glu Glu Ala Asp Lys Gly Asn Ser His Ser Lys Lys Met Leu 
    130                 135                 140                 


Lys Ala Leu Leu Ser Glu Gly Glu Ser Ile Trp Glu Ile Thr Glu Lys 
145                 150                 155                 160 


Ile Leu Asn Ser Phe Glu Tyr Thr Ser Arg Phe Thr Lys Thr Lys Thr 
                165                 170                 175     


Leu Tyr Gln Phe Leu Phe Leu Ala Thr Phe Ile Asn Cys Gly Arg Phe 
            180                 185                 190         


Ser Asp Ile Lys Asn Val Asp Pro Lys Ser Phe Lys Leu Val Gln Asn 
        195                 200                 205             


Lys Tyr Leu Gly Val Ile Ile Gln Cys Leu Val Thr Glu Thr Lys Thr 
    210                 215                 220                 


Ser Val Ser Arg His Ile Tyr Phe Phe Ser Ala Arg Gly Arg Ile Asp 
225                 230                 235                 240 


Pro Leu Val Tyr Leu Asp Glu Phe Leu Arg Asn Ser Glu Pro Val Leu 
                245                 250                 255     


Lys Arg Val Asn Arg Thr Gly Asn Ser Ser Ser Asn Lys Gln Glu Tyr 
            260                 265                 270         


Gln Leu Leu Lys Asp Asn Leu Val Arg Ser Tyr Asn Lys Ala Leu Lys 
        275                 280                 285             


Lys Asn Ala Pro Tyr Ser Ile Phe Ala Ile Lys Asn Gly Pro Lys Ser 
    290                 295                 300                 


His Ile Gly Arg His Leu Met Thr Ser Phe Leu Ser Met Lys Gly Leu 
305                 310                 315                 320 


Thr Glu Leu Thr Asn Val Val Gly Asn Trp Ser Asp Lys Arg Ala Ser 
                325                 330                 335     


Ala Val Ala Arg Thr Thr Tyr Thr His Gln Ile Thr Ala Ile Pro Asp 
            340                 345                 350         


His Tyr Phe Ala Leu Val Ser Arg Tyr Tyr Ala Tyr Asp Pro Ile Ser 
        355                 360                 365             


Lys Glu Met Ile Ala Leu Lys Asp Glu Thr Asn Pro Ile Glu Glu Trp 
    370                 375                 380                 


Gln His Ile Glu Gln Leu Lys Gly Ser Ala Glu Gly Ser Ile Arg Tyr 
385                 390                 395                 400 


Pro Ala Trp Asn Gly Ile Ile Ser Gln Glu Val Leu Asp Tyr Leu Ser 
                405                 410                 415     


Ser Tyr Ile Asn Arg Arg Ile 
            420             

